ORAL COMPOSITION COMPRISING B-ESCIN AND THE USE THEREOF

Abstract

The present invention relates to a field of treatment and prevention of overweight and obesity. In particular the present invention is directed to an oral composition comprising (beta)-escin (beta-escin) for use in prevention of extracellular to intracellular water ratio (ECW/ICW) increase in weight loss interventions in treatment of overweight and obesity. Therefore, the composition of the invention has a beneficial effect on fluid distribution during weight loss. The composition of the invention not only improves ECW/ICW ratio but also induces an anorectic effect and results in appetite suppression. Therefore, it is useful alone as overweight and obesity intervention.

Claims

1. Oral composition comprising an active ingredient and pharmaceutically acceptable excipients, wherein the active ingredient is β-escin, which is present in the composition in the dose from 0.015 to 0.20 mg/kg per day, for use in prevention of extracellular to intracellular water ratio increase in weight loss interventions for treatment of overweight and obesity.

2. The composition for use of claim 1, further comprising chokeberry fruit extract.

3. The composition for use of claim 1, wherein the composition 1s for administration up to five times per day, preferably up to three times per day, most preferably once a day.

4. The composition for use of claim 1, wherein the composition is for administration during or after a meal.

5. The composition for use of claim 1, wherein the composition is for administration once per day during or after the first meal of a day.

6. The composition for use of claim 1, wherein the composition is for administration five times per day during or after any meal.

7. The composition for use of claim 1, wherein the composition is in a solid form, preferably in a form of a tablet, capsule, powder or granules

8. The composition for use according claim 1, wherein the composition is in a liquid form, preferably in a form of a solution.

9. The composition for use of claim 8, wherein β-escin is present at a concentration between 20 to 200 mg/l, preferably between 50 mg/l to 75 mg/l, even more preferably at a concentration of 50 mg/l.

10. The composition for use of claim 8, wherein β-escin is dissolved in the solvent selected from water or ethanol, preferably the solvent is water.

11. The composition for use of claim 1 further comprising one or more additives selected from flavourings, colourings, preservatives, vitamins, sweeteners, antioxidants, and minerals.

12. The composition for use of claim 1, further comprising an anti-foaming agent and/or CO.sub.2.

13. The composition for use of claim 1, wherein said composition constitutes the only weight loss intervention for treatment of overweight and obesity.

14. The composition for use of claim 1, wherein daily dose of β-escin amounts from 2.5 mg to 8.5 mg, preferably amounts 4 mg to 6 mg, most preferably amounts to 5 mg.

15. The composition for use of claim 1, wherein the active ingredient is β-escin is present in the composition in the dose from 0.03 to 0.10 mg/kg per day.

Description

BRIEF DESCRIPTION OF DRAWINGS

[0018] The subject of the invention was illustrated in drawings.

[0019] FIG. 1 displays graphically the fat content in stool obtained from overweight subjects following the consumption of the composition of invention.

[0020] FIG. 2 displays graphically reduction of a fat content observed in obese subjects during consumption of the composition of the invention, wherein E is escin and AE is chokeberry juice concentrate with escin.

[0021] FIGS. 3A-3D displays graphically the TBW %, ECW %, ICW % and ECW/ICW ratio observed in overweight and obese subjects during consumption of the composition of the invention, wherein E is β-escin and AE is chokeberry juice concentrate with β-escin.

DETAILED DESCRIPTION OF THE INVENTION

[0022] Compositions and methods of the invention are useful in the prevention and treatment of overweight and obesity by inducing appetite suppression leading to a potent and sustained decrease in food intake and body weight loss. β-escin used in a composition of the present invention is a mixture of triterpene saponins isolated from horse chestnut seeds (genus Aesculus, for example Aescuhus hippocasanum, L.). Although ethnopharmacological research provides evidence for its broad use to treat numerous diverse disorders, including bladder diseases, cough, diarrhea, dysmenorrhea and tinnitus, its current use is restricted mainly to venotonic and venoprotective indications. Despite the therapeutic significance of β-escin and the popularity of the drug, which in the United States and Europe remains one of the best-selling herbal extracts accounting for 226 million U.S. dollar-market in 2014 (IMS Kilochem), its exact mechanism of action remains to be fully elucidated.

[0023] The present inventor has observed and confirmed in trials that the composition according present invention has surprising effects in appetite suppression with concomitant weight loss, therefore it could be used in the prevention and treatment of overweight and obesity. Advantageously, the composition permits to reduce food intake due to its anorectic effect.

[0024] The highest dose of β-escin present in the composition according to the invention, when it is in a liquid form, amounts to 200 mg/l. However, the preferred concentration of β-escin in the liquid composition is 180 mg/l. Most preferably 50 mg/l of β-escin is provided to a subject in a in a single dose of 20 ml during or immediately after a meal, wherein the composition is administered up to five times a day, which amounts preferably to the dose of 5 mg of β-escin per day. The relative amount (dose) of β-escin in the composition is significant since this active ingredient is a potent inhibitor of pancreatic lipase. High dosing can result in steatorrhea or at the very least undesirable abdominal pain. It is therefore important that the dose of this compound is sufficient to achieve the desired therapeutic effect of suppressing appetite without inherently causing unwanted side effects. It is therefore very common that drugs for venous insufficiency containing high doses of escin (most commonly 20 mg in a form of tablet or capsule, taken 3 times a day to reach total daily dose of 120-240 mg) are indicated to be taken either two hours prior to a meal or three hours after a meal (https://www.doz.pl/ieki/p1902-Aescin_tabletki).

[0025] First nutraceutical activities of escins were described by Kimura et al. who demonstrated their inhibitory action on pancreatic lipase and predicted anti-obese effects (Identification of novel saponins from edible seeds of Japanese horse chestnut (Aesculus turbinata Blume) after treatment with wooden ashes and their nutraceutical activity. Kimura et al. Journal of Pharmaceutical and Biomedical Analysis, 2006, 41, 1657-1665). Further data obtained from animal studies indicated that the anti-obesity effects were observed in mice fed with diets containing very high escins-containing saponin fraction (0,1%-0,5%), i.e. a mouse of approximately 25-30 g in weight consumed daily 5-25 mg of saponins (Antiobese Effects of Novel Saponins from Edible Seeds of Japanese Horse Chestnut (Aesculus turbinata BLUME) after Treatment with Wood Ashes. Kimura et al., J. Agric. Food Chem. 2008, 56, 4783-4788). As clearly stated by the authors, the attenuating effect on body weight resulting from saponin treatment was not due to the reduced food intake but due to an inhibitory effect on lipase activity. Similar conclusions can be drawn from another animal study in which the authors used even higher doses of escins (up to 2% in diet in mice and up to 1 g/kg body in rats): the change in body weight was not associated with decreased food consumption and was attributed to the inhibition of pancreatic lipase activity (Anti-obesity Effects of Escins Extracted from the Seeds of Aesculus turbinata BLUME (Hippocastanaceae). Hu et al., Chem. Pharm. Bull. 2008, 56, 12-16). Very high dose of escin, i.e. 100 mg/kg was administered to mice also in a study by Avci et al. (Effects of escin mixture from the seeds of Aesculus hippocastanum on obesity in mice fed a high fat diet. Avci et al. Pharmaceutical Biology, 2010, 48, 247-252). In concordance with the results of earlier studies, while the authors described the beneficiary effect of escin on the body weight in rats fed a high fat diet, they did not report any effect of the executed treatment on the food consumption. It should be underlined that in animal studies β-escin was administered in doses higher by three orders of magnitude as compared to the composition of invention.

[0026] Very limited and not unequivocal human data linking escin treatment to appetite suppression comes mainly from patent documents. Polish patent application P.405318 discloses the use of composition comprising very low dose of β-escin (2 mg/I) and chokeberry fruit extract (200 g/l) to induce weight loss and describes diminished appetite in human subjects following the treatment. However, this publication does not provide sufficient information to clearly conclude that the observed weight loss and appetite inhibition is related to β-escin, and particularly β-escin provided in very low doses summing up to only 1 mg per day. As shown in Examples s-escin consumed by human subjects in a dose of 1 mg per day had no effect on lipid content in stool (FIG. 1) and on satiety score. It is therefore more plausible that the effect described in P.405318 results from chokeberry component which has proven metabolic effects. Chokeberry extract is isolated from the fruits of Aronia melanocarpa, known i.e. for its antioxidant, hypocholesterolemic and hypoglycemic properties. Administration of chokeberry extract to diabetic rats attenuate hyperglycemia and hypertriglyceridemia (Hypoglycemic and hypolipidemic effects of Aronia melanocarpa fruit juice in streptozotocin-induced diabetic rats. Valcheva-Kuzmanova, S. et al., Methods Find Exp Clin Pharmacol, 2007, 29, 101-5). These effects, at least in part, are attributed to the natural phenolic constituents, which are beneficial in improving carbohydrate and lipid metabolism. Significant decrease in the absorption of nutrients as well as body weight gain was observed in animals fed a diet supplemented with chokeberry extract (Comparative Effect of Green Tea, Chokeberry and Honeysuckle Polyphenols on Nutrients and Mineral Absorption and Digestibility in Rats. Frejnagel S. and Wroblewska M., Ann Nutr Metab 2010; 56:163-169). Even more important seem data showing that in rats fed with a fructose diet, which is a well-accepted animal model of obesity, extracts of Aronia improved blood glucose and serum lipid profile, reduced adipose tissue mass and body weight gain by regulating multiple genes in adipogenesis, glucose, lipid and inflammatory pathways. These included mRNA expression of CD36— a fatty acid transporter, glycogen synthase and TNF-α (An extract of chokeberry attenuates weight gain and modulates insulin, adipogenic and inflammatory signalling pathways in epididymal adipose tissue of rats fed a fructose-rich diet. Qin B. and Anderson R. A. British Journal of Nutrition, 2012, 108, 581-587). In line with the above quoted articles remains patent application US 2010/0222422 A1 (T. J. Romero, B. Q., P. J. Miller, Dietary supplements containing extracts of Aronia and methods of using same to promote weight loss). Furthermore, there are claims linking phytochemical constituents which comprise many edible berry fruits to increased satiety (Impact of bioflavonoids from berryfruits on biomarkers of metabolic syndrome. Lila M. A., Functional Foods in Health and Disease 2011, 1, 13-24).

[0027] In patent application US20080220101A1 β-escin is mentioned as one of components of compositions containing extracts of Aloe vera to induce satiety and/or reduce the appetite. Although β-escin does not appear in none of the claims of the patent document, it is mentioned in the detailed description of the invention as the active component of Castanea sativa—sweet chestnut and in the document wrongly named a horse chestnut. Castanea sativa is known for its antioxidant properties but it is not recognized as β-escin source, while it is the horse chestnut (genus Aesculus) that is a main known source of β-escin. Although due to the confusing description it is not clear if β-escin is included in the formulation, the purpose of including β-escin in the formulation is not revealed. Finally, the influence of plant extracts on feeding behaviour was tested in moth Spodoptera littoralis and not human subjects.

[0028] Therefore, the strong appetite suppressing effect of the β-escin containing composition of the invention, observed in human subjects, should be considered as unexpected and could not be predicted from previously published studies.

[0029] The liquid composition of the invention comprising β-escin, at a concentration of less than 200 mg/l is disclosed for the first time in use as a medicament. The composition is particularly useful in the prevention or treatment of overweight and obesity resulting from appetite suppression and has beneficial effect on fluids distribution in overweight and obese subjects during weight loss.

[0030] The term “weight loss interventions” should be understood as any treatment method resulting in patient's weight loss, including but not limited to diet, surgical, pharmaceutical and psychological methods or combinations thereof. In view of the above, the term “treatment” should be understood as any process resulting in patient's weight loss, and which comprises any actions that leads to the required goal such as, for example, lifestyle management, pharmacotherapy and surgery. Further it can be understood as the use of an agent, procedure, or regimen, such as a diet, drug, surgery, or exercise, in an attempt to cure or mitigate a disease or condition associated with overweight and obesity.

[0031] The terms “overweight” and “obesity” are defined as abnormal or excessive fat accumulation that presents a health risk. A crude population measure of obesity is the body mass index (BMI), a person's weight (in kilograms) divided by the square of his or her height (in metres). A person with a BMI between 25-30 is considered overweight, and a person with a BMI of or more is generally considered obese.

[0032] The term “chokeberry fruit extract” as used herein refers to any solid or liquid formulation obtained from chokeberry fruits. The liquid formulation might be, for example, a chokeberry fruit juice. In one preferred embodiment a chokeberry fruit extract is in a dried extract form. In other preferred embodiments, the composition of the invention comprises a chokeberry fruit juice concentrate. The chokeberry fruit extract, including fruit juice concentrate, may be provided in any form readily known to the skilled person, for example it may be in a dried extract form. Such juice concentrates are readily commercially available and characterised by a Brix value, wherein the extract typically comprises 65-68° Bx. Any suitable alternative supply sufficient to provide the same concentrate of chokeberry concentrate or extract is envisaged.

[0033] The following studies illustrate the most important findings related to the composition of invention.

Example 1: In-Vivo Studies

Satiation Evaluation

[0034] Satiation is the process that brings a meal to an end. Verbal reports on the processes that bring a meal to an end indicate that ‘fullness’ and ‘boredom with taste’ are two major reasons to stop eating. In the experiment, 30 healthy overweight adults were randomly assigned to three groups receiving: 1) composition of invention (1 mg S-escin in water); 2) composition comprising 0.2 mg β-escin in water; 3) placebo (sodium chloride). The subjects were instructed to take 20 ml of the provided composition after each main meal and two snacks, in total five times a day. As satiation is measured through the measurement of ad libitum food consumption, no food restrictions were advised. Throughout the 21-day, real-life study, at day 7, 14 and 21 the subjects were asked to complete five primary scales for self-reported appetite:

1) Hunger. The question: How hungry are you? The low and high anchor: “Not at all” and “Extremely”, respectively;
2) Fullness. The question: How full are you? The low and high anchor: Not at all” and “Extremely”, respectively;
3) Satiety. The question: How satiated are you? The low and high anchor: Not at all” and “Extremely”, respectively:
4) Desire. The question: How strong is your desire to eat? The low and high anchor: “Very weak” and “Very strong”, respectively;
5) Prospective consumption. The question: How much do you think you could eat right now?The low and high anchor: “Nothing at all” and “A very large amount”, respectively.

[0035] The scales were linear (100 mm with 10 points where 1 was the low, and 10 the high anchor) and they were completed before and after the evening meal on the indicated days.

[0036] After the 21-day experiment and 7-day break, each group was assigned randomly for the new treatment, so at the end of the experiment each subject completed the scales for all the tested compositions.

[0037] The analysis of study results clearly indicated that only the group receiving the composition of invention reported significant hunger suppression, increased fullness and satiety, reduced desire to eat and declared strongly diminished prospective consumption (10 out of 10). In majority of cases (8 out of 10) the results of the treatment were observed on day 21, while the in two of the cases the results were observed on day 14 and on day 7, respectively. Importantly, all the subjects noted that the experienced changes led to reduced food consumption and therefore to reduced meal sizes. In the other groups only single cases of hunger suppression, increased fullness and satiety, reduced desire to eat and diminished prospective consumption were noted.

Example 1A. Fat Content in Stool

[0038] To further investigate the unexpected satiety increasing and appetite decreasing effect of the β-escin, the inventor performed the measurement of lipid content in stool. It is well established that β-escin is an inhibitor of lipase activity and may lead to steatorrhea (fatty stools) in administered in high doses. In the performed tests, four obese patients were asked to ingest composition of invention (1 mg R-escin in 20 ml water); 0.2 mg β-escin in 20 ml water or 5 mg β-escin in 20 ml water, after each meal and two snacks, i.e. 5 times a day. The stool samples were collected on day 2. One-week break was ensured between the different treatments. As shown in the Figure, consumption of 5 mg and 25 mg of β-escin per day (i.e. five doses of 1 mg or 5 mg) led to considerable increase in the lipid content in stool.

[0039] However, the patients receiving the dose of 5 mg surprisingly did not notice any discomfort related to the increased lipid content in stool, whereas the patients receiving the highest dose of β-escin (25 mg per day) noticed symptoms such as loose stools, particularly after meals with high fat content, which was confirmed in the biochemical analyses.

Example 1B. Weight Loss

[0040] The group of 30 overweight (BMI 25.0-29.9) and obese (BMI>30) volunteers having body weight from 54 to 146 kg was recruited for a 3-month study (the initial treatment) aiming to assess the weight loss efficacy of the composition of invention (0.5-1.5 mg β-escin in water five times a day). Subsequently, in the next 3-month period 10 volunteers did not receive any treatment, 10 took the composition of invention only once a day after the biggest meal (2.5-8.5 mg 3-escin per day in water) and 10 continued the initial treatment (0.5-1.5 mg β-escin in water five times a day). Importantly, at the time when the first dose of the composition of invention was administered, all the study subjects were consuming their normal diet. At the end of the first 3-month initial treatment: all of the 30 volunteers lost weight, of which 28 (93%) reached or surpassed the 5% value indicating a clinically significant weight loss. Further weight reduction was observed in both groups during the continuing treatment, although the values for the subjects continuing the initial treatment were higher as compared to the subjects receiving the composition of invention only once a day after the biggest meal (additional 5% vs 2% of initial body weight, mean values). In the group not receiving the treatment no weight regain was observed and in 4 subjects further slight weight loss was noted (1% of initial body weight). Moreover, in groups receiving the treatment reduced food consumption has been observed.

[0041] The results indicate that the composition of invention can be used as an efficient aid in weight management in overweight and obese subjects. Furthermore, no correlation between the BMI and weight loss efficacy was detected which indicates that β-escin content in the composition of invention (2.5-8.5 mg β-escin per day) is equally effective in overweight and obese patients and it is not necessary to scale up the dosage in adjustment to patient weight.

Example 2

Vitamin D Levels

[0042] Due to a potential adverse effect of β-escin consumption on the absorption of fat soluble vitamins, related to β-escin induced reduction in lipid absorption, the inventor monitored the level of vitamin D (vitamin 25(OH)D) in randomly assigned six subjects treated with the composition of invention. During the duration of the tests vitamin D concentration in blood was assessed three times: at the beginning of the study prior to β-escin consumption, after 6 weeks and at the end of the study, i.e. after three months. The obtained results demonstrated that the treatment with the composition of invention had no effect on vitamin 25(OH)D concentration, which remained stable throughout the study in the range of ≥30-50 ng/ml (i.e. ≥75-125 nmol/l).

Example 3

Fat Content Reduction in Obese Subjects

[0043] Body composition was assessed by a Bioelectrical Impedance Analyser (BIA) BioScan (Maltron International Ltd) device. The manufacturer's in-build pre-programmed equations were used to measure body fat percentage (FAT/o). All volunteers in the study were of BMI ≥30 (obese). As depicted in FIG. 2, 12-week treatment with the composition of invention (1 mg β-escin in water) with or without chokeberry juice concentrate (5 g) 3-5 times a day after meals had no clear effect on fat tissue reduction.

Example 4

Fluid Distribution in Overweight and Obese Subjects and its Modifications During Weight Loss

[0044] Body composition was assessed by a Bioelectrical Impedance Analyser (BIA) BioScan (Maltron International Ltd) device. The manufacturer's in-build pre-programmed equations were used to measure the percentage of total body water, as well as extracellular and intracellular water (TBW %, ECW % and ICW %). All volunteers in the study were of BMI ≥25 (overweight and obese) and they consumed the tested compositions 3-5 times a day after meals. As shown in FIG. 3A, the formulation of the composition, i.e. β-escin alone or with chokeberry juice concentrate had no effect on TBW %. However, surprisingly and unexpectedly there were differences between the evaluated groups in the ECW %, ICW % and in consequence in ECW/ICW ratio. Formulation containing β-escin and chokeberry juice concentrate induced much more noticeable reduction in ECW % as compared to β-escin alone (FIG. 3 B), but had negligible effect on ICW %, whereas β-escin alone led to certain decrease in ICW % (FIG. 3 C). Consequently, the ECW/ICW ratio in the volunteers receiving composition containing 1 mg β-escin and 5 g chokeberry juice concentrate dropped, whereas in volunteers receiving composition of the present invention (1 mg β-escin in water) the ratio ECW/ICW remained unchanged. Changing aspects of fluid distribution parameters observed in both groups provide valid information about beneficial weight loss modifications obtained with β-escin, and particularly evident when used with chokeberry juice concentrate. According to current understanding of fluid distribution in obese subjects and its modifications during weight loss, a relative expansion of extracellular volume and higher ECW/ICW ratios do no normalize in individuals who lose weight, on the contrary, body fluid alterations progress. In the light of the above, β-escin induced appetite suppression leading to satisfactory weight loss and blocking, or as in case of β-escin with chokeberry juice concentrate—reversing fluid alterations represents an important invention in safe weight loss interventions.

[0045] The theoretical basis together with the in-vitro and human studies carried out by the inventor demonstrate a sound basis that the composition of the invention provides useful technical effects when administered to subjects, especially humans, but also animals. Due to the efficacy of the composition of the invention in weight management and no added sugars it may be advised as well for diabetic patients and elderly patients, as in prophylaxis against cardiovascular disease related to weight gain.