COMPOSITION FOR PROMOTING THE GROWTH OF LACTIC ACID BACTERIA COMPRISING GROWTH FACTORS

Abstract

The present disclosure relates to a composition for promoting the growth of lactic acid bacteria comprising growth factors, and more particularly, to a composition for promoting the growth of lactic acid bacteria comprising growth factors, the composition being capable of efficiently promoting the growth of various lactic acid bacteria strains.

Claims

1. A method for promoting the growth of lactic acid bacteria, the method comprising culturing the lactic acid bacteria in a medium; wherein the medium comprises glucose, fructooligosaccharide, yeast extract and isolated soy protein.

2. The method of claim 1, wherein the medium comprises 20 to 40 wt % of the glucose, 20 to 40 wt % of the fructooligosaccharide, 10 to 30 wt % of the yeast extract, and 5 to 30 wt % of the isolated soy protein.

3. The method of claim 1, wherein a weight ratio of the glucose and the fructooligosaccharide is 1:3 to 3:1.

4. The method of claim 1, wherein a weight ratio of the yeast extract and the isolated soy protein is 1:3 to 3:1.

5. The method of claim 1, wherein the lactic acid bacteria is selected from at least one group consisting of Lactobacillus casei, Lactobacillus plantarum, Lactobacillus rhamnosus, Bifidobacterium longum, Bifidobacterium lactis, Bifidobacterium bifidum, Streptococcus thermophilus and Pediococcus pentosaceus.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0021] FIG. 1 shows the results of evaluating the effect of treatment with a composition for promoting the growth of lactic acid bacteria comprising growth factors on the growth of Lactobacillus casei CBT LC5.

[0022] FIG. 2 shows the results of evaluating the effect of treatment with the composition for promoting the growth of lactic acid bacteria comprising growth factors on the growth of Bifidobacterium longum CBT BG7.

[0023] FIG. 3 shows the results of evaluating the effect of treatment with the composition for promoting the growth of lactic acid bacteria comprising growth factors on the growth of Bifidobacterium bifidum CBT-BF3.

[0024] FIG. 4 shows the results of evaluating the effect of treatment with the composition for promoting the growth of lactic acid bacteria comprising growth factors on the growth of Streptococcus thermophilus CBT-ST3.

DESCRIPTION OF SPECIFIC EMBODIMENTS

[0025] The specific embodiments described herein are representative of preferred embodiments or examples of the present disclosure, and thus the scope of the present disclosure is not limited thereto. It will be apparent to those skilled in the art that modifications and other uses of the present disclosure do not depart from the scope of the present disclosure as defined in the appended claims.

EXAMPLES

Experimental Methods

Example: Selection of Optimal Composition

[0026] To promote the growth of non-specific various types of lactic acid bacteria, an optimal mixture composition was determined by culturing each of the following strains using each component, ratio and composition and measuring the viable cell count of each of the strains: Lactobacillus casei CBT-LC5 (accession number: KCTC 12398BP), Lactobacillus plantarum CBT-LP3 (accession number: KCTC 10782BP), Lactobacillus rhamnosus CBT-LR5 (accession number: KCTC 12202BP), Bifidobacterium longum CBT-BG7 (accession number: KCTC 12200BP), Bifidobacterium lactis CBT-BL3 (accession number: KCTC 11904BP), Bifidobacterium bifidum CBT-BF3 (accession number: KCTC 12199BP), Streptococcus thermophilus CBT-ST3 (accession number: KCTC 11870BP), and Pediococcus pentosaceus CBT-SL4 (accession number: KCTC 10297BP). Starter cultures were prepared using BL broth.

Experimental Example 1: Experiment of Selection of Carbon Source

[0027] As shown in Table 1 below, 2 parts by weight of each carbon source was added to 100 parts by weight of BL broth, thereby preparing media.

TABLE-US-00001 TABLE 1 Control Refined Fructo- Strain name (BL broth) Glucose Fructose sugar Lactose oligosaccharid Lactobacillus casei CBT LC5 0% 2% 2% 2% 2% 2% Lactobacillus plantarum CBT LP3 0% 2% 2% 2% 2% 2% Lactobacillus rhamnosus CBT LR5 0% 2% 2% 2% 2% 2% Bifidobacterium longum CBT BG7 0% 2% 2% 2% 2% 2% Bifidobacterium lactis CBT BL3 0% 2% 2% 2% 2% 2% Bifidobacterium bifidum CBT BF3 0% 2% 2% 2% 2% 2% Streptococcus thermophilus CBT ST3 0% 2% 2% 2% 2% 2% Pediococcus pentosaceus CBT SL4 0% 2% 2% 2% 2% 2%

[0028] 0.1 to 1 part by weight of each of the starter cultures was inoculated into 100 parts by weight of each prepared medium, and then cultured for 20 hours while the pH was maintained at about 5.0 to 6.5. Next, the viable cell count of each culture was measured, and the results of the measurement are summarized in Table 2 below. As a result, it was confirmed that culture with glucose or fructooligosaccharide exhibited an excellent effect on the growth of the lactic acid bacteria. Thus, glucose or fructooligosaccharide was selected as a carbon source for a subsequent experiment (Table 2).

TABLE-US-00002 TABLE 2 (unit: ×10.sup.7 cfu/ml) Control Refined Fructo- Strain name (BL broth) Glucose Fructose sugar Lactose oligosaccharide Lactobacillus casei CBT LC5 348 650 408 482 405 615 Lactobacillus plantarum CBT LP3 318 613 452 427 550 616 Lactobacillus rhamnosus CBT LR5 212 615 486 425 405 580 Bifidobacterium longum CBT BG7 363 612 405 464 456 570 Bifidobacterium lactis CBT BL3 410 770 522 472 420 715 Bifidobacterium bifidum CBT BF3 306 640 434 325 460 660 Streptococcus thermophilus CBT ST3 280 620 402 504 450 640 Pediococcus pentosaceus CBT SL4 322 650 550 580 520 702

Experimental Example 2: Experiment for Combination of Carbon Sources

[0029] As shown in Table 3 below, glucose and fructooligosaccharide were mixed together at the indicated ratios, and then 2 parts by weight of each mixture was added to 100 parts by weight of BL broth, thereby preparing media.

TABLE-US-00003 TABLE 3 Control 1:1 1:2 1:3 2:1 3:1 Strain name (BL broth) 50%:50% 33%:67% 25%:75% 67%:33% 75%:25% Lactobacillus casei CBT LC5 0% 2% 2% 2% 2% 2% Lactobacillus plantarum CBT LP3 0% 2% 2% 2% 2% 2% Lactobacillus rhamnosus CBT LR5 0% 2% 2% 2% 2% 2% Bifidobacterium longum CBT BG7 0% 2% 2% 2% 2% 2% Bifidobacterium lactis CBT BL3 0% 2% 2% 2% 2% 2% Bifidobacterium bifidum CBT BF3 0% 2% 2% 2% 2% 2% Streptococcus thermophilus CBT ST3 0% 2% 2% 2% 2% 2% Pediococcus pentosaceus CBT SL4 0% 2% 2% 2% 2% 2%

[0030] 0.1 to 1 part by weight of each starter culture was inoculated into 100 parts by weight of each prepared medium, and then cultured for 20 hours while the pH was maintained at about 5.0 to 6.5. Next, the viable cell count of each culture was measured, and the results of the measurement are summarized in Table 4 below. As a result, it was confirmed that culture with glucose and fructooligosaccharide at the same mixing ratio (50%:50%) exhibited a better effect on the growth of the lactic acid bacteria than culture at the other mixing ratios. Thus, the mixing ratio of 50:50 was selected as a mixing ratio for a subsequent experiment (Table 4).

TABLE-US-00004 TABLE 4 (unit: ×10.sup.7 cfu/ml) Control 1:1 1:2 1:3 2:1 3:1 Strain name (BL broth) 50%:50% 33%:67% 25%:75% 67%:33% 75%:25% Lactobacillus casei CBT LC5 356 725 616 608 620 618 Lactobacillus plantarum CBT LP3 326 726 612 610 625 660 Lactobacillus rhamnosus CBT LR5 255 735 621 622 621 612 Bifidobacterium longum CBT BG7 330 722 650 680 640 650 Bifidobacterium lactis CBT BL3 436 729 610 615 610 620 Bifidobacterium bifidum CBT BF3 302 715 670 680 587 650 Streptococcus thermophilus CBT ST3 310 712 660 620 600 668 Pediococcus pentosaceus CBT SL4 346 732 680 655 670 680

Experimental Example 3: Experimental for Selection of Nitrogen Source

[0031] As shown in Table 5 below, 2 parts by weight of each nitrogen source was added to 100 parts by weight of BL broth, thereby preparing media.

TABLE-US-00005 TABLE 5 Control (BL Yeast Nonfat dry Isolated soy Strain name broth) extract milk protein Lactobacillus casei CBT LC5 0% 2% 2% 2% Lactobacillus plantarum CBT LP3 0% 2% 2% 2% Lactobacillus rhamnosus CBT LR5 0% 2% 2% 2% Bifidobacterium longum CBT BG7 0% 2% 2% 2% Bifidobacterium lactis CBT BL3 0% 2% 2% 2% Bifidobacterium bifidum CBT BF3 0% 2% 2% 2% Streptococcus thermophilus CBT ST3 0% 2% 2% 2% Pediococcus pentosaceus CBT SLA 0% 2% 2% 2%

[0032] 0.1 to 1 part by weight of each starter culture was inoculated into 100 parts by weight of each prepared medium, and then cultured for 20 hours while the pH was maintained at about 5.0 to 6.5. Next, the viable cell count of each culture was measured, and the results of the measurement are summarized in Table 6 below. As a result, it was confirmed that culture with yeast extract or isolated soy protein exhibited a better effect on the growth of the lactic acid bacteria than the other nitrogen sources. Thus, yeast extract or isolated soy protein was selected for a subsequent experiment (Table 6).

TABLE-US-00006 TABLE 6 (unit: × 10.sup.7 cfu/ml) Control (BL Yeast Nonfat dry Isolated soy Strain name broth) extract milk protein Lactobacillus casei CBT LC5 355 670 560 680 Lactobacillus plantarum CBT LP3 325 820 615 885 Lactobacillus rhamnosus CBT LR5 226 750 645 890 Bifidobacterium longum CBT BG7 342 752 670 725 Bifidobacterium lactis CBT BL3 442 815 775 806 Bifidobacterium bifidum CBT BF3 301 670 604 770 Streptococcus thermophilus CBT ST3 295 620 805 720 Pediococcus pentosaceus CBT SL4 315 722 715 780

Experimental Example 4: Experiment for Combination of Nitrogen Sources

[0033] As shown in Table 7 below, yeast extract and isolated soy protein were mixed together at the indicated ratios, and then 2 parts by weight of each mixture was added to 100 parts by weight of BL broth, thereby preparing media.

TABLE-US-00007 TABLE 7 Control 1:1 1:2 1:3 2:1 3:1 Strain name (BL broth) 50%:50% 33%:67% 25%:75% 67%:33% 75%:25% Lactobacillus casei CBT LC5 0% 2% 2% 2% 2% 2% Lactobacillus plantarum CBT LP3 0% 2% 2% 2% 2% 2% Lactobacillus rhamnosus CBT LR5 0% 2% 2% 2% 2% 2% Bifidobacterium longum CBT BG7 0% 2% 2% 2% 2% 2% Bifidobacterium lactis CBT BL3 0% 2% 2% 2% 2% 2% Bifidobacterium bifidum CBT BF3 0% 2% 2% 2% 2% 2% Streptococcus thermophilus CBT ST3 0% 2% 2% 2% 2% 2% Pediococcus pentosaceus CBT SLA 0% 2% 2% 2% 2% 2%

[0034] 0.1 to 1 part by weight of each starter culture was inoculated into 100 parts by weight of each prepared medium, and then cultured for 20 hours while the pH was maintained at about 5.0 to 6.5. Next, the viable cell count of each culture was measured, and the results of the measurement are summarized in Table 8 below. As a result, it was confirmed that culture with yeast extract and isolated soy protein at a mixing ratio of 3:1 (75%: 25%) exhibited a better effect on the growth of the lactic acid bacteria than culture at the other mixing ratios. Thus, the mixing ratio of 3:1 was selected as a mixing ratio for a subsequent experiment (Table 8).

TABLE-US-00008 TABLE 8 (unit: ×10.sup.7 cfu/ml) Control 1:1 1:2 1:3 2:1 3:1 Strain name (BL broth) 50%:50% 33%:67% 25%:75% 67%:33% 75%:25% Lactobacillus casei CBT LC5 348 690 670 680 690 702 Lactobacillus plantarum CBT LP3 318 830 863 872 886 895 Lactobacillus rhamnosus CBT LR5 235 760 845 854 863 906 Bifidobacterium longum CBT BG7 318 762 733 745 755 825 Bifidobacterium lactis CBT BL3 422 822 785 796 803 816 Bifidobacterium bifidum CBT BF3 317 702 725 734 752 802 Streptococcus thermophilus CBT ST3 322 705 715 720 725 810 Pediococcus pentosaceus CBT SL4 336 780 765 740 750 833

Experimental Example 5: Experiment for Selection of Optimal Mixture Composition Comprising Four Different Components

[0035] As shown in Table 9 below, glucose, fructooligosaccharide, yeast extract and isolated soy protein were mixed together at the indicated ratios, and then 2 parts by weight of each of the mixtures was added to 100 parts by weight of BL broth, thereby preparing media.

TABLE-US-00009 TABLE 9 Control 25%:25%: 20%:20%: 30%:30%: 40%:40%: 40%:40%: Strain name (BL broth) 25%:25% 30%:30% 20%:20% 10%:10% 15%:5% Lactobacillus casei CBT LC5 0% 2% 2% 2% 2% 2% Lactobacillus plantarum CBT LP3 0% 2% 2% 2% 2% 2% Lactobacillus rhamnosus CBT LR5 0% 2% 2% 2% 2% 2% Bifidobacterium longum CBT BG7 0% 2% 2% 2% 2% 2% Bifidobacterium lactis CBT BL3 0% 2% 2% 2% 2% 2% Bifidobacterium bifidum CBT BF3 0% 2% 2% 2% 2% 2% Streptococcus thermophilus CBT ST3 0% 2% 2% 2% 2% 2% Pediococcus pentosaceus CBT SL4 0% 2% 2% 2% 2% 2%

[0036] 0.1 to 1 part by weight of each starter culture was inoculated into 100 parts by weight of each of the prepared media, and then cultured for 20 hours while the pH was maintained at about 5.0 to 6.5. Next, the viable cell count of each culture was measured, and the results of the measurement are summarized in Table 10 below. As a result, it was confirmed that, as the content of the carbon source was higher than the content of the nitrogen source, the effect of the carbon source and the nitrogen source on the growth of the lactic acid bacteria increased, and when 40 wt % glucose, 40 wt % fructooligosaccharide, 15 wt % yeast extract and 5 wt % isolated soy protein were mixed together, the mixture exhibited the best effect on the growth of the lactic acid bacteria.

TABLE-US-00010 TABLE 10 (unit: ×10.sup.7 cfu/ml) Control 25%:25%: 20%:20%: 30%:30%: 40%:40%: 40%:40%: Strain name (BL broth) 25%:25% 30%:30% 20%:20% 10%:10% 15%:5% Lactobacillus casei CBT LC5 344 770 760 820 970 1,070 Lactobacillus plantarum CBT LP3 334 746 735 810 935 1,040 Lactobacillus rhamnosus CBT LR5 243 762 732 832 975 1,030 Bifidobacterium longum CBT BG7 312 740 725 825 925 1,050 Bifidobacterium lactis CBT BL3 419 730 720 760 910 1,100 Bifidobacterium bifidum CBT BF3 305 700 710 840 922 1,020 Streptococcus thermophilus CBT ST3 345 680 700 752 912 1,120 Pediococcus pentosaceus CBT SL4 332 775 732 796 948 1,050

Experimental Example 6: Experiment for Verification of Optimal Mixture Composition

[0037] To finally verify the effect of the optimal mixture composition, mixtures of two or three of the four components were compared with the final mixture composition. As shown in Table 11 below, glucose, fructooligosaccharide, yeast extract and isolated soy protein were mixed together at various ratios as follows: Comparative Example 1 (not comprising), Comparative Example 2 (75% yeast extract and 25% isolated soy protein), Comparative Example 3 (80% glucose, 15% yeast extract, and 5% isolated soy protein), Comparative Example 4 (80% fructooligosaccharide, 15% yeast extract, and 5% isolated soy protein), and Example (40% glucose, 40% fructooligosaccharide, 15% yeast extract, and 5% isolated soy protein). Then, 2 parts by weight of each of the mixtures was added to 100 parts by weight of BL broth, thereby preparing media.

TABLE-US-00011 TABLE 11 Comparative Example 1 Comparative Comparative Comparative Strain name (not comprising) Example 2 Example 3 Example 4 Example Lactobacillus casei CBT LC5 0% 2% 2% 2% 2% Lactobacillus plantarum CBT LP3 0% 2% 2% 2% 2% Lactobacillus rhamnosus CBT LR5 0% 2% 2% 2% 2% Bifidobacterium longum CBT BG7 0% 2% 2% 2% 2% Bifidobacterium lactis CBT BL3 0% 2% 2% 2% 2% Bifidobacterium bifidum CBT BF3 0% 2% 2% 2% 2% Streptococcus thermophilus CBT ST3 0% 2% 2% 2% 2% Pediococcus pentosaceus CBT SL4 0% 2% 2% 2% 2%

[0038] 0.1 to 1 part by weight of each starter culture was inoculated into 100 parts by weight of each of the prepared media, and then cultured for 20 hours while the pH was maintained at about 5.0 to 6.5. Next, the viable cell count of each culture was measured, and the results of the measurement are summarized in Table 12 below. In addition, the growth curve of each strain was analyzed by measuring the number of viable cells at 2-hour intervals for 20 hours (FIGS. 1 to 4). As a result, it was confirmed that culture with the composition of the Example (the composition comprising the mixture of glucose, fructooligosaccharide, yeast extract and isolated soy protein) exhibited an excellent effect of promoting the growth of all the eight types of lactic acid bacteria compared to culture with each of the compositions of Comparative Examples 1 to 4, and it could be confirmed that the composition of the Example exhibited not only the effects of activating the growth of the lactic acid bacteria and promoting cell division, but also the stability effect of allowing the proliferated lactic acid bacteria to survive for a long time (Table 12 and FIGS. 1 to 4).

TABLE-US-00012 TABLE 12 (unit: × 10.sup.7 cfu/ml) Comparative Example 1 Comparative Comparative Comparative Strain name (not comprising) Example 2 Example 3 Example 4 Example Lactobacillus casei CBT LC5 363 702 715 722 1,040 Lactobacillus plantarum CBT LP3 327 895 920 915 1,030 Lactobacillus rhamnosus CBT LR5 252 906 935 944 1,050 Bifidobacterium longum CBT BG7 316 825 837 872 1,050 Bifidobacterium lactis CBT BL3 399 816 845 882 1,090 Bifidobacterium bifidum CBT BF3 312 802 822 850 1,070 Streptococcus thermophilus CBT ST3 317 810 825 838 1,040 Pediococcus pentosaceus CBT SLA 343 833 826 872 1,000

[0039] As described in detail above, the composition for promoting the growth of lactic acid bacteria comprising growth factors according to the present disclosure may promote the growth of lactic acid bacteria, and thus may be used universally as a composition for promoting the growth of various lactic acid bacteria.

[0040] Although the present disclosure has been described in detail with reference to the specific features, it will be apparent to those skilled in the art that this description is only of a preferred embodiment thereof, and does not limit the scope of the present disclosure. Thus, the substantial scope of the present disclosure will be defined by the appended claims and equivalents thereto.

[0041] [Accession Number]

[0042] Depository authority: Korea Research Institute of Bioscience and Biotechnology

[0043] Accession number: KCTC12199BP

[0044] Deposit date: Apr. 27, 2012

[0045] Depository authority: Korea Research Institute of Bioscience and Biotechnology

[0046] Accession number: KCTC12200BP

[0047] Deposit date: Apr. 27, 2012

[0048] Depository authority: Korea Research Institute of Bioscience and Biotechnology

[0049] Accession number: KCTC11904BP

[0050] Deposit date: Mar. 30, 2011

[0051] Depository authority: Korea Research Institute of Bioscience and Biotechnology

[0052] Accession number: KCTC12398BP

[0053] Deposit date: Apr. 5, 2013

[0054] Depository authority: Korea Research Institute of Bioscience and Biotechnology

[0055] Accession number: KCTC10782BP

[0056] Deposit date: Mar. 16, 2005

[0057] Depository authority: Korea Research Institute of Bioscience and Biotechnology

[0058] Accession number: KCTC12202BP

[0059] Deposit date: Apr. 27, 2012

[0060] Depository authority: Korea Research Institute of Bioscience and Biotechnology

[0061] Accession number: KCTC10297BP

[0062] Deposit date: Jun. 25, 2002

[0063] Depository authority: Korea Research Institute of Bioscience and Biotechnology

[0064] Accession number: KCTC11870BP

[0065] Deposit date: Feb. 17, 2011