COLORING ADDITIVE

Abstract

The invention relates to a coloring additive for food or feed, comprising a water extract of sweet chestnut wood comprising sweet chestnut tannins. The additive can be in granular or liquid form. The amount of tannins in the sweet chestnut wood extract is about 40 wt % or more, preferably 40 to 60 wt % expressed as total polyphenols, calculated on dry matter. The coloring additive is a.o. suitable as replacement of caramel color class IV in soft drinks.

Claims

1. Food or feed product comprising a coloring additive, the coloring additive being a water extract of sweet chestnut wood, the extract comprising sweet chestnut tannins.

2. The product according to claim 1, wherein the amount of tannins in the sweet chestnut wood extract is about 40 wt % or more expressed as total polyphenols, calculated on dry matter.

3. The product according to claim 1, wherein the tannins in the sweet chestnut wood extract comprise gallic acid and ellagic acid, and hydrolysable esters therefrom with monosaccharides.

4. The product according to claim 1, wherein the product is a food product, which is a cola drink concentrate.

5. The product according to claim 4, wherein the coloring agent is present in 0.1-1 wt %.

6. The product according to claim 1, wherein the product is a food product, which is a cola drink.

7. The product according to claim 6, wherein the coloring agent is present in 0.0001-0.05 wt %.

8. Use of a water extract of sweet chestnut wood, the extract comprising sweet chestnut tannins, as a coloring agent in human food or animal feed.

9. Use according to claim 8, wherein the coloring additive is in granular form.

10. Use according to claim 8, wherein the additive is in liquid form

11. Use according to claim 8, wherein the amount of tannins in the sweet chestnut wood extract is about 40 wt % or more expressed as total polyphenols, calculated on dry matter.

12. Use according to claim 8, wherein the tannins in the sweet chestnut wood extract comprise gallic acid and ellagic acid, and hydrolysable esters therefrom with monosaccharides.

Description

DETAILED DESCRIPTION

[0017] The coloring agent according to the present invention comprises a water extract of sweet chestnut wood comprising sweet chestnut tannins. The wood is also called bark, no difference is intended.

[0018] In one embodiment of the invention, the coloring agent is in solid form, like powder or granules.

[0019] In another embodiment of the invention, the coloring agent is in the form of a liquid.

[0020] Water extract of sweet chestnut wood comprises tannins. These tannins are largely not condensed. Tannins from chestnut wood are known as a food additive, leather tanning processes, feed additive and the like. Tannins are used as feed additive in poultry to lower bacterial pressure, with little effect on growth (see e.g. Poultry Science (2008) 87:521-527). In the use as feed additive, the extract generally is mixed as powder through other feed ingredients.

[0021] The amount of tannins in the water extract generally is about 40 wt % or more, preferably between 40-60 wt %, more preferably 45 and 55 wt % expressed as total polyphenols and with a dominant part as ellagic acid (or esters therefrom), calculated on dry matter. Higher concentrated extracts would be suitable as well. Tannins from sweet chestnut wood comprise gallic acid and ellagic acid, and hydrolysable esters therefrom with monosaccharides, like vescalin, castalin, vescalagin and castalagin.

[0022] The water extract of sweet chestnut wood for use as coloring agent according the present invention can be obtained by water extraction of chestnut wood. Water extraction is well known, and such water extracts are commercially available. Examples of suitable sources of tannin include: Silvafeed (Silva srl—Italy), Farmatan or Globatan (Tanin Sevnica—Slovenia), King Brown or Tanno-SAN (King Tree France, Sanluc Belgium). The product generally is supplied as powder or granule, but can also be used in liquid form, with e.g. about 50 wt % solids.

[0023] The extraction of tannins from sweet chestnut wood does not require any chemical modification, and the resulting tannin extract is therefore a fully biological additive. This is a distinction with extracts from chestnut shells, that require alcohol or other organic solvents.

[0024] The amount of sweet chestnut concentrate in the food or feed product can vary, and may be as high as 2 wt % or less. Preferably, the amount will be about 1 wt % or less.

[0025] The amount of sweet chestnut extract, comprising the sweet chestnut tannin, in a soft drink concentrate generally will be about 0.1%-0.8% of commercially available dry chestnut extract dissolved in the soft drink. Preferably, the amount is about 0.2-0.5 wt %. The sweet chestnut extract may be diluted with e.g. bulking material, in which case the amounts will be calculated relative to the chestnut extract only.

[0026] The sweet chestnut extract can be used as coloring agent in combination with other, preferably natural, coloring agents. Suitable coloring agents include scorpion yellow pigment, cochineal red pigment, natural orange red pigment, cocoa shell pigment, grape skin pigment, safflower yellow pigment, malt pigment, curcumin and their compounds and grade I caramel color if made without non-natural components.

[0027] Color ratio measurement can be done as follows: prepare a 0.1% sample dilution. Use the 0.1% sample dilution in a lcm cuvette with distilled water as a reference, and measure the optical density OD1 value at 610 nm with UV spectrophotometer. Preferably, the measurement is repeated three times, and the average OD1 value is used, The EBC color strength is calculated as follows: Color rate (EBC unit)=OD1×20,000/0.076

[0028] The red index can be determined in an analogous way: Use a wavelength of 510 nm in the optical density measurement and measure an OD2, and the red index is calculated as follows: Red index=10×(OD2/OD1).

EXAMPLES

[0029] Two water extracts of bark of sweet chestnut were compared with caramel type IV (E150d).

[0030] The dry matter content was measured after drying for 48 hr at 105 dgr C. The results were as follows:

TABLE-US-00001 Dry matter (n = 3) g/100 g RSD Product of original SD (%) Tannin Powder 92.54 0.07 0.1 Liquid Tannin 46.22 0.02 0.0 Liquid Caramel 50.16 0.05 0.1 type IV

[0031] Color measurements were done according to the standard practice, measuring the optical density of 0.1 wt % caramel, dissolved in ultra-pure water at pH 6.2. The result was a value of 0.253±0.002 (n=3). Next, tannin solutions were prepared of 1.2% (m/v) in ultra pure water (pH=6.2). After filtration (because the tannin extracts were slightly hazy, the optical density was measured, at a dilution range down to 0.1 wt %. Next, the amount was determined to achieve the same optical density as the caramel type IV product. The results were as follows:

TABLE-US-00002 Coloring strength (n = 3) % RSD Product (m/v) SD (%) Tannin Powder 0.86 0.03 3.1 Liquid Tannin 1.04 0.09 9.0 Liquid Caramel 0.1 Reference type IV

[0032] Further characterization revealed that the amount of hydroxymethyl furfural was one tenth or less in the tannin extracts when compared with the liquid caramel type IV. The much lower value of this hydroxy-aldehyde lowers the risk of unwanted Maillard reactions, and increases stability.

[0033] Further characterization showed that the amount of polyphenols was 15-20 times higher in the tannin products.

[0034] Next, the antioxidant activity of the tannin extracts was compared with caramel type IV. The radical DPPH (1,1-Diphenyl-2-picryhydrazyl) was used. The reduction of the radical DPPH by an antioxidant can be measured with a spectrophotometer. The reduction of the absorbance at 517 nm is induced by the presence of an antioxidant. DPPH is initially violet, and decolorizes when the free electron disappears. Such decolouration is representative of the antioxidant capacity of the measured species independent of any enzymatic activity.

[0035] Measurements were performed such that the IC50 was determined. Results are as follows:

TABLE-US-00003 IC50 (n = 3) μg μg RSD Product (on original) SD (on dry wt) SD (%) Tannin Powder 31.2 1.0 28.8 0.9 3.1 Liquid Tannin 55.3 1.8 25.5 0.8 3.2 Liquid Caramel 897.6 113.4 450.0 56.8 12.6 type IV

[0036] These experiments show, that next to the coloring agent being completely natural (no chemical used for extraction), it has health benefits because of good antioxidant properties.