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IMPROVED METHOD FOR THE PRODUCTION OF HIGH LEVELS OF PUFA IN PLANTS

20210317467 · 2021-10-14

    Inventors

    Cpc classification

    International classification

    Abstract

    The present invention is concerned with materials and methods for the production of genetically modified plants, particularly where the plants are for the production of at least one unsaturated or polyunsaturated fatty acid. The invention is also concerned with identification of genes conveying an unsaturated fatty acid metabolic property to a plant or plant cell, and generally relates to the field of phosphotidylcholine:diacylglycerol cholinephosphotransferase (PDCT).

    Claims

    1. A plant raw oil comprising PUFAs, e.g. vIcPUFAs, as for example, EPA, DHA, and/or DPA that further comprises i. a level of the 18:2 fatty acid in % (w/w) in the triacylglycerol (TAG) fraction that is between 80 and 120% of the level as the 18:2 fatty acid level in % (w/w) in the diacylglyerol (DAG) fraction, ii. a level of the 22:1 fatty acid in % (w/w) in the triacylglycerol fraction that is higher than the 22:1 fatty acid level in % (w/w) in the diacylglyerol fraction, and/or iii. a level of SDA in % (w/w) in the phosphatyidylcholine (PC) fraction is higher than the SDA level in % (w/w) in the triacylglycerol fraction.

    2. A method for the production of a composition comprising the fatty acid GLA in a plant, or part thereof, like a plant cell, and/or part seed, or part thereof, wherein the level of the 18:2 fatty acid in % (w/w) in the triacylglycerol (TAG) fraction is around the same level as the 18:2 fatty acid level in % (w/w) in the diacylglyerol (DAG) fraction, comprising, providing a plant cable to produce GLA and having an increased activity or expression of one or more PDCT compared to the wild type, the PDCT selected from the group consisting of: (a) a PDCT1 having at least 80% sequence identity with SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46; (b) a PDCT1 encoded by a polynucleotide having at least 80% sequence identity with SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 39, 41, 43, and/or 45; (c) a PDCT1 encoded by a polynucleotide that hybridizes under high stringency conditions with (i) a polynucleotide that encodes the amino acid sequence of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46, or (ii) the full-length complement of (i); (d) a variant of the PDCT1 of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46 comprising a substitution, deletion, and/or insertion at one or more positions and having PDCT1 activity; (e) a PDCT1 encoded by a polynucleotide that differs from SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 39, 41, 43, and/or 45 due to the degeneracy of the genetic code; and (f) a fragment of the PDCT1 of (a), (b), (c), (d) or (e) having PDCT1 activity; and, optionally, the seed oil is isolated.

    3. Method for increasing the Delta-6 elongase conversion efficiency in a plant, plant cell, plant seed and/or part thereof, that is capable to produce PUFA and expresses a Delta-6 elongase, comprising increasing, compared to a control, in the plant, plant cell, plant seed and/or part thereof the activity or expression rate of one or more PDCT selected from the group consisting of: (a) a PDCT1 having at least 80% sequence identity with SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46; (b) a PDCT1 encoded by a polynucleotide having at least 80% sequence identity with SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 39, 41, 43, and/or 45; (c) a PDCT1 encoded by a polynucleotide that hybridizes under high stringency conditions with (i) a polynucleotide that encodes the amino acid sequence of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46, or (ii) the full-length complement of (i); (d) a variant of the PDCT1 of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46 comprising a substitution, deletion, and/or insertion at one or more positions and having PDCT1 activity; (e) a PDCT1 encoded by a polynucleotide that differs from SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 39, 41, 43, and/or 45 due to the degeneracy of the genetic code; and (f) a fragment of the PDCT1 of (a), (b), (c), (d) or (e) having PDCT1 activity.

    4. Method for improving the cellular conversion efficiency from oleic acid to C18 to C22 PUFA in a plant, plant seed, plant cell or part thereof, comprising providing a plant, plant cell, plant seed or part thereof, that is capable to produce C18 to C22 PUFA, comprising increasing the activity of one or more PDCT selected from the group consisting of: (a) a PDCT1 having at least 80% sequence identity with SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46; (b) a PDCT1 encoded by a polynucleotide having at least 80% sequence identity with SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 39, 41, 43, and/or 45; (c) a PDCT1 encoded by a polynucleotide that hybridizes under high stringency conditions with (i) a polynucleotide that encodes the amino acid sequence of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46, or (ii) the full-length complement of (i), (d) a variant of the PDCT1 of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46 comprising a substitution, preferably a conservative substitution, deletion, and/or insertion at one or more positions and having PDCT1 activity; (e) a PDCT1 encoded by a polynucleotide that differs from SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 39, 41, 43, and/or 45 due to the degeneracy of the genetic code; and (f) a fragment of the PDCT1 of (a), (b), (c), (d) or (e) having PDCT1 activity. and expressing a phospholipid-dependent desaturase and, optionally, at least one Acetyl-CoA-dependent desaturase

    5. A method for producing vIcPUFA in an oil crop plant, comprising providing a first an oil crop plant variety that is cable to produce the desired vlcPUFA, providing a second an oil crop plant variety that has an increased activity of one or more PDCT selected from the group consisting of: (a) a PDCT1 having at least 80% sequence identity with SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46; (b) a PDCT1 encoded by a polynucleotide having at least 80% sequence identity with SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 39, 41, 43, and/or 45; (c) a PDCT1 encoded by a polynucleotide that hybridizes under high stringency conditions with (i) a polynucleotide that encodes the amino acid sequence of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46, or (ii) the full-length complement of (i); (d) a variant of the PDCT1 of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46 comprising a substitution, deletion, and/or insertion at one or more positions and having PDCT1 activity; (e) a PDCT1 encoded by a polynucleotide that differs from SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 39, 41, 43, and/or 45 due to the degeneracy of the genetic code; and (f) a fragment of the PDCT1 of (a), (b), (c), (d) or (e) having PDCT1 activity; crossing the first and second an oil crop plant variety, optionally, measuring the PDCT1 expression rate in first or later generation cells, seeds, plants or part thereof derived from the cross, optionally, measuring the total PUFA level in in first or later generation cells, seeds, plants or part thereof derived from the cross, optionally, repeating steps 2 to 5, planting and growing the plant, and isolating the vIcPUFA comprising oil from the seed of first or later generation plants derived from the cross.

    6. Method for the production of a composition comprising the fatty acids GLA in a plant, or part thereof, plant cell, and/or part seed, or part thereof, cable to produce GLA comprising providing a plant, plant cell or seed with an increased activity or expression of one or more PDCT selected from the group consisting of: (a) a PDCT1 having at least 80% sequence identity with SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46; (b) a PDCT1 encoded by a polynucleotide having at least 80% sequence identity with SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 39, 41, 43, and/or 45; (c) a PDCT1 encoded by a polynucleotide that hybridizes under high stringency conditions with (i) a polynucleotide that encodes the amino acid sequence of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46, or (ii) the full-length complement of (i); (d) a variant of the PDCT1 of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46 comprising a substitution, deletion, and/or insertion at one or more positions and having PDCT1 activity; (e) a PDCT1 encoded by a polynucleotide that differs from SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 39, 41, 43, and/or 45 due to the degeneracy of the genetic code; and (f) a fragment of the PDCT1 of (a), (b), (c), (d) or (e) having PDCT1 activity; and, optionally, isolating the composition comprising the desired fatty acids.

    7. The method of claim 6, whereby the plant, plant seed or plant cell expresses at least one phospholipid-dependent desaturased.

    8. The method or oil of claim 1, whereby the plant, plant seed or plant cell expresses at least one phospholipid-dependent desaturase and at least one Acetyl-CoA-dependent desaturase.

    9. The method or oil of claim 1, whereby the plant, plant seed or plant cell expresses at least one acyl-CoA dependent desaturase and/or at least one PC-dependent elongase selected from the group consisting of d5-, d5d6-, and d6elongase.

    10. The method or oil of claim 1, whereby the plant, plant seed or plant cell expresses at least one Delta-6 elongase and/or at least one Delta-6-desaturase.

    11. The method or oil of claim 1, wherein the total PUFA level in the plant, plant seed, plant cell or an oil derived therefrom is increased compared to a control.

    12. The method or oil of claim 1, wherein the ratio of C20 fatty acids to C18 is increased.

    13. The method or oil of claim 1, wherein a composition isolated from the plant the plant or cell is derived that is a raw oil.

    14. A method or oil of claim 1 comprising, providing a plant, seed, or plant cell capable to produce acids EPA, DPA and/or DHA, the plant, seed, or plant cell comprising: at least one nucleic acid sequence which encodes at least one Delta-12 desaturase at least one nucleic acid sequence which encodes at least one Omega 3 desaturase, at least one nucleic acid sequence which encodes a Delta 6-desaturase activity, b) at least one nucleic acid sequence which encodes a Delta-6 elongase activity, c) at least one nucleic acid sequence which encodes a Delta-5 desaturase activity, d) at least one nucleic acid sequence which encodes a Delta-5 elongase activity, and e) at least one nucleic acid sequence which encodes a Delta-4 desaturase activity

    15. The method or oil of claim 1, wherein an oil is produced with a ratio of 18:1 fatty acids to total fatty acid content (w/w) is reduced by at least 10% compared to the control and/or wherein ratio ALA to total fatty acid content is reduced by at least 20%.

    16. The method or oil of claim 1, wherein at least one of the following PDCT has an increased activity or is overexpressed: napus_1A, napus_2A, napus_1C, and/or napus_2C PDCT when the plant, plant cell or plant seed is a Brassica sp., or a B. napus plant, plant cell or plant seed carinata_1B, carinata_1C, carinata_2B, and/or carinata_2C PDCT when the plant, plant cell or plant seed is a Brassica sp., or a B. carinata plant, plant cell or plant seed, or BjROD1-B4, BjROD1-A3, and/or BjROD1-B3 PDCT, when the plant, plant cell or plant seed is a Brassica sp., or a B. juncea plant, plant cell or plant seed.

    17. The method or oil of claim 1, comprising, compared to a control, in the plant or part thereof, or seed, or plant cell the increasing the activity or expressing a PDCT of one or more PDCT selected from the group consisting of: a) a PDCT1 having at least 80% sequence identity with SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46; (b) a PDCT1 encoded by a polynucleotide having at least 80% sequence identity with SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 39, 41, 43, and/or 45; (c) a PDCT1 encoded by a polynucleotide that hybridizes under high stringency conditions with (i) a polynucleotide that encodes the amino acid sequence of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46, or (ii) the full-length complement of (i), (d) a variant of the PDCT1 of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46 comprising a substitution, deletion, and/or insertion at one or more positions and having PDCT1 activity; (e) a PDCT1 encoded by a polynucleotide that differs from SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 39, 41, 43, and/or 45 due to the degeneracy of the genetic code; and (f) a fragment of the PDCT1 of (a), (b), (c), (d) or (e) having PDCT1 activity; and, optionally, isolating the composition comprising the desired fatty acids. and one or more PDCT selected from the group consisting of: (a) a PDCT1 having at least 80% sequence identity with SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46; (b) a PDCT1 encoded by a polynucleotide having at least 80% sequence identity with SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 39, 41, 43, and/or 45; (c) a PDCT1 encoded by a polynucleotide that hybridizes under high stringency conditions with (i) a polynucleotide that encodes the amino acid sequence of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46, or (ii) the full-length complement of (i); (d) a variant of the PDCT1 of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46 comprising a substitution, deletion, and/or insertion at one or more positions and having PDCT activity; (e) a PDCT1 encoded by a polynucleotide that differs from SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 39, 41, 43, and/or 45 due to the degeneracy of the genetic code; and (f) a fragment of the PDCT of (a), (b), (c), (d) or (e) having PDCT1 activity

    18. The method or oil of claim 1, wherein the delta 6 elongase conversion efficiency is increased

    19. The method or oil of any one claim 1, wherein the expression or activity of at least one PDCT3 and/or a PDCT5 is reduced, the PDCT3 and/or a PDCT5 selected from (a) PDCT3 and/or PDCT5 having at least 80% sequence identity with SEQ ID NO: 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60; (b) PDCT3 and/or PDCT5 encoded by a polynucleotide having at least 80% sequence identity with SEQ ID NO: 17, 19, 21, 23, 27, 29, 31, 49, 51, 53, 55, and/or 57; (c) PDCT3 and/or PDCT5 encoded by one or more polynucleotides that hybridize under high stringency conditions with (i) a polynucleotide that encodes the amino acid sequence of SEQ ID NO: 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60, or (ii) the full-length complement of (i); (d) variants of the PDCT3 and/or PDCT5 of SEQ ID NO: 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60, comprising a substitution, preferably a conservative substitution, deletion, and/or insertion at one or more positions and having PDCT3 and/or PDCT5 activity; (e) PDCT3 and/or PDCT5 encoded by a polynucleotide that differs from SEQ ID NO: 17, 19, 21, 23, 27, 29, 31, 49, 51, 53, 55, and/or 57 due to the degeneracy of the genetic code; and (f) fragments of the PDCT3 and/or PDCT5 of (a), (b), (c), (d) or (e) having PDCT3 and/or PDCT5 activity.

    20. The method or oil of claim 1, whereby a heterologous PDCT1 or PDCT19 is expressed de novo.

    21. A method for the production of food or feed comprising EPA, DHA and/or DPA, isolating the fatty acid composition and formulating the fatty acid composition to food or feed.

    22. An isolated, a synthetic, or a recombinant polynucleotide comprising: (a) a nucleic acid sequence having at least 80% sequence identity to SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 39, 41, 43, and/or 45, wherein the nucleic acid encodes a polypeptide having PDCT1 activity; (b) a nucleic acid sequence encoding a polypeptide having at least 80% sequence identity to SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46, wherein the polypeptide has PDCT1 activity; (c) a fragment of (a) or (b), wherein the fragment encodes a polypeptide having PDCT1 activity; or (d) a nucleic acid sequence fully complementary to any of (a) to (c).

    23. An isolated, a synthetic, or a recombinant polynucleotide comprising polynucleotide of claim 22 and: (a) a nucleic acid sequence having at least 80% sequence identity to SEQ ID NO: 35, 37, or 47, wherein the nucleic acid encodes a polypeptide having PDCT19 activity; (b) a nucleic acid sequence encoding a polypeptide having at least 80% sequence identity to SEQ ID NO: 36, 38, or 48, wherein the polypeptide has PDCT19 activity; (c) a fragment of (a) or (b), wherein the fragment encodes a polypeptide having PDCT19 activity; or (d) a nucleic acid sequence fully complementary to any of (a) to (c).

    24. A nucleic acid construct comprising a polynucleotide of claim 22, operably linked to one or more heterologous control sequences that directs the expression of the protein of interest in a cell, preferably in a plant cell.

    25. A vector comprising the polynucleotide of claim 22 or a nucleic acid construct.

    26. A host cell comprising a polynucleotide of claim 22, a nucleic acid construct or a vector.

    27. The host cell of claim 26, wherein said host cell is selected from the group consisting of Agrobacterium, yeast, bacterial, algae or plant cell.

    28. A method of producing the polypeptide of selected from the groups consisting of: (a) a PDCT1 having at least 80% sequence identity with SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46; (b) a PDCT1 encoded by a polynucleotide having at least 80% sequence identity with SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 39, 41, 43, and/or 45; (c) a PDCT1 encoded by a polynucleotide that hybridizes under high stringency conditions with (i) a polynucleotide that encodes the amino acid sequence of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46, or (ii) the full-length complement of (i), (d) a variant of the PDCT1 of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46 comprising a substitution, deletion, and/or insertion at one or more positions and having PDCT1 activity; (e) a PDCT1 encoded by a polynucleotide that differs from SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 39, 41, 43, and/or 45 due to the degeneracy of the genetic code; and (f) a fragment of the PDCT1 of (a), (b), (c), (d) or (e) having PDCT1 activity or the polynucleotide of claim 22, comprising the steps of (i) providing a host cell, (ii) cultivating the host cell of step (a) under conditions conductive for the production of the polypeptide or the polynucleotide in the host cell; and (iii) optionally, recovering the polypeptide or or the polynucleotide.

    29. A method for the production of a transgenic plant, plant cell, plant seed, a part thereof, or an oil thereof, having an increased amount of GLA and/or an increase in total PUFA and/or an increased ratio of C20 fatty acids to C18, and/or an increased the conversion rate of a Delta-6 elongase relative to control plants, said method comprising: (i) introducing and expressing in a plant, or part thereof, or plant cell, or plant seed a nucleic acid encoding a polypeptide as defined in claim 28; and (ii) cultivating said plant cell or plant under conditions an increased amount of GLA and/or an increase in total PUFA and/or an increased ratio of C20 fatty acids to C18, and/or an increased the conversion rate of a Delta-6 elongase relative to control plants.

    30. A method for the production of a transgenic plant, plant cell, plant seed, a part thereof, or an oil thereof, having an increased amount of GLA and/or an increase in total PUFA and/or an increased ratio of C20 fatty acids to C18, and/or an increased the conversion rate of a Delta-6 elongase relative to control plants, said method comprising (i) replacing in a plant cell or plant a regulatory element controlling the endogenous expression of the polypeptide as defined in claim 28 or of an endogenous nucleic acid molecule encoding the polypeptide by a replacement regulatory element that increases the expression of the endogenous polypeptide or of the endogenous nucleic acid molecule encoding the polypeptide; and (ii) cultivating said plant cell or plant under conditions promoting an increased amount of GLA and/or an increase in total PUFA and/or an increased ratio of C20 fatty acids to C18, and/or an increased the conversion rate of a Delta-6 elongase relative to control plants.

    31. A transgenic plant, or part thereof, or plant cell, or plant seed obtainable by a method according to claim 29.

    32. A transgenic plant, or part thereof, or plant cell, or plant seed having an increased amount of GLA and/or an increase in total PUFA and/or an increased ratio of C20 fatty acids to C18, and/or an increased the conversion rate of a Delta-6 elongase relative to control plants, resulting from the increased activity or expression rate of the PDCT as defined in claim 29 in a seed cell.

    33. A transgenic plant, or part thereof, or plant cell, or plant seed according to claim 31, wherein said transgenic plant, or part thereof, or plant cell, or plant seed is a Camelina seed or a Brassica sp seed.

    34. A transgenic plant, or part thereof, or plant cell, or plant seed comprising a nucleic acid construct of claim 24.

    35. A transgenic plant according to claim 31, or a transgenic plant cell or a plant seed derived therefrom, wherein said plant, or cell or seed is an oil crop plant.

    36. The plant of claim 31, wherein the expression or activity of at least one PDCT3 and/or a PDCT5 is reduced, the PDCT3 and/or a PDCT5 selected from (a) PDCT3 and/or PDCT5 having at least 80% sequence identity with SEQ ID NO: 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60; (b) PDCT3 and/or PDCT5 encoded by a polynucleotide having at least 80% sequence identity with SEQ ID NO: 17, 19, 21, 23, 27, 29, 31, 49, 51, 53, 55, and/or 57; (c) PDCT3 and/or PDCT5 encoded by one or more polynucleotides that hybridize under high stringency conditions with (i) a polynucleotide that encodes the amino acid sequence of SEQ ID NO: 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60, or (ii) the full-length complement of (i); (d) variants of the PDCT3 and/or PDCT5 of SEQ ID NO: 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60, comprising a substitution, deletion, and/or insertion at one or more positions and having PDCT3 and/or PDCT5 activity; (e) PDCT3 and/or PDCT5 encoded by a polynucleotide that differs from SEQ ID NO: 17, 19, 21, 23, 27, 29, 31, 49, 51, 53, 55, and/or 57 due to the degeneracy of the genetic code; and (f) fragments of the PDCT3 and/or PDCT5 of (a), (b), (c), (d) or (e) having PDCT3 and/or PDCT5 activity.

    37. A protein preparation comprising the polypeptide of claim 29, wherein the protein preparation comprises a lyophilized composition/formulation and/or additional enzymes or compounds.

    38. A plant raw oil derived from the plant of claim 31.

    39. A plant raw oil having an increased amount of GLA and/or an increase in total PUFA and/or an increased ratio of C20 fatty acids to C18.

    40. An antibody or a fragment thereof specifically binding to the polypeptide as defined in claim 28.

    41. A product derived or produced from a harvestable part of a plant of claim 31, wherein the product is a dry pellet, a pulp pellet, a pressed stem, a meal, a powder, or a fibre, containing a composition produced from the plant; or wherein the product comprises an oil, a fat, a fatty acid, a carbohydrate, or a starch, a sap, a juice, a molasses, a syrup, a chaff, or a protein produced from the plant.

    42. (canceled)

    43. A feed or food product comprising the plant oil of claim 38.

    44. The feed composition of claim 43, comprising no oil derived from animals.

    45. A method for the production of a composition comprising the fatty acid 22:1 in a plant, or part thereof, like a plant cell, and/or part seed, or part thereof, wherein the level of the 22:1 fatty acid in % (w/w) in the triacylglycerol fraction is higher than the 22:1 fatty acid level in % (w/w) in the diacylglyerol fraction, comprising, providing a plant cable to produce GLA and having an increased activity or expression of one or more PDCT compared to the wild type, the PDCT selected from the group consisting of: (a) a PDCT1 having at least 80% sequence identity with SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46; (b) a PDCT1 encoded by a polynucleotide having at least 80% sequence identity with SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 39, 41, 43, and/or 45; (c) a PDCT1 encoded by a polynucleotide that hybridizes under high stringency conditions with (i) a polynucleotide that encodes the amino acid sequence of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46, or (ii) the full-length complement of (i), (d) a variant of the PDCT1 of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46 comprising a substitution, deletion, and/or insertion at one or more positions and having PDCT1 activity; (e) a PDCT1 encoded by a polynucleotide that differs from SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 39, 41, 43, and/or 45 due to the degeneracy of the genetic code; and (f) a fragment of the PDCT1 of (a), (b), (c), (d) or (e) having PDCT1 activity;

    46. A method for the production of a composition comprising the fatty acid SDA in a plant, or part thereof, like a plant cell, and/or part seed, or part thereof, wherein the level of SDA in % (w/w) in the phosphatyidylcholine (PC) fraction is higher than the SDA level in % (w/w) in the triacylglycerol fraction, comprising, providing a plant cable to produce SDA and having an increased activity or expression of one or more PDCT compared to the wild type, the PDCT selected from the group consisting of: (a) a PDCT1 having at least 80% sequence identity with SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46; (b) a PDCT1 encoded by a polynucleotide having at least 80% sequence identity with SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 39, 41, 43, and/or 45; (c) a PDCT1 encoded by a polynucleotide that hybridizes under high stringency conditions with (i) a polynucleotide that encodes the amino acid sequence of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46, or (ii) the full-length complement of (i); (d) a variant of the PDCT1 of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46 comprising a substitution, preferably a conservative substitution, deletion, and/or insertion at one or more positions and having PDCT1 activity; (e) a PDCT1 encoded by a polynucleotide that differs from SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 39, 41, 43, and/or 45 due to the degeneracy of the genetic code; and (f) a fragment of the PDCT1 of (a), (b), (c), (d) or (e) having PDCT1 activity;

    47. Method for increasing the level of DPA, DHA and/or EPA in a plant, a part thereof, a plant cell, and/or plant seed, that is capable to produce DPA, DHA and/or EPA and, comprising providing a plant, a part thereof, a plant cell, and/or plant seed expressing a Delta-6 elongase and having an increasing the endogenous PDCT activity or expressing a heterologous PDCT or of one or more PDCT selected from the group consisting of: (a) a PDCT1 having at least 80% sequence identity with SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46; (b) a PDCT1 encoded by a polynucleotide having at least 80% sequence identity with SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 39, 41, 43, and/or 45; (c) a PDCT1 encoded by a polynucleotide that hybridizes under high stringency conditions with (i) a polynucleotide that encodes the amino acid sequence of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46, or (ii) the full-length complement of (i); (d) a variant of the PDCT1 of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46 comprising a substitution, deletion, and/or insertion at one or more positions and having PDCT1 activity; (e) a PDCT1 encoded by a polynucleotide that differs from SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 39, 41, 43, and/or 45 due to the degeneracy of the genetic code; and (f) a fragment of the PDCT1 of (a), (b), (c), (d) or (e) having PDCT1 activity;

    48. A method to produce a plant or a part thereof, the plant cell, and/or the plant seed that comprises an oil that is characterized by i. the level of the 18:2 fatty acid in % (w/w) in the triacylglycerol (TAG) fraction that is between 80 and 120% of the level as the 18:2 fatty acid level in % (w/w) in the diacylglyerol (DAG) fraction ii. the total PUFAs is increased, iii. a ratio of 18:1 fatty acids to total fatty acid content (w/w) is 10% less compared to the control and/or wherein ratio ALA to total fatty acid content is reduced by at least 20%. lv: an increased amount of GLA, v. an increased ratio of C20 fatty acids to C18, vi. the level of the 22:1 fatty acid in % (w/w) in the triacylglycerol fraction is higher than the 22:1 fatty acid level in % (w/w) in the diacylglyerol fraction, vii. the level of SDA in % (w/w) in the phosphatyidylcholine (PC) fraction is higher than the SDA level in % (w/w) in the triacylglycerol fraction

    49. Method for the production of a plant, a part thereof, a plant cell, plant seed and/or plant seed oil, wherein the total PUFAs level is increased compared to a control, comprising increasing, in a plant, a part thereof, a plant cell, and/or plant seed the activity or expression rate of one or more PDCT compared to the control, wherein the PDCT is selected from the group consisting of: (a) a PDCT1 having at least 80% sequence identity with SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46; (b) a PDCT1 encoded by a polynucleotide having at least 80% sequence identity with SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 39, 41, 43, and/or 45; (c) a PDCT1 encoded by a polynucleotide that hybridizes under high stringency conditions with (i) a polynucleotide that encodes the amino acid sequence of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46, or (ii) the full-length complement of (i); (d) a variant of the PDCT1 of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46 comprising a substitution, deletion, and/or insertion at one or more positions and having PDCT1 activity; (e) a PDCT1 encoded by a polynucleotide that differs from SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 39, 41, 43, and /or 45 due to the degeneracy of the genetic code; and (f) a fragment of the PDCT1 of (a), (b), (c), (d) or (e) having PDCT1 activity; and isolating the seed oil.

    Description

    FIGURES

    [0495] FIG. 1 Alignment of PDCT protein sequences

    [0496] Legend: At: Arabidopsis thaliana, Bn: Brassica napus, Bc: Brassica carinata, Cs: Camelina sativa, Gm: Glycine max, Lu: Linum usitatissimum, Rc: Ricinus communis, Ta: Triticum aestivum, Zm: Zea mays.

    [0497] * activity demonstrated in other studies

    [0498] ** proteins selected based on homology in BLAST searches of NCBI databases, activity not demonstrated

    [0499] Color setup: Non-similar, weakly similar: dark grey, conserved: light grey, blocks of similar: medium grey, identical: white

    [0500] FIG. 2 Alignment of N-terminal region of C. sativa sequences. All differences in the C. satvia proteins are within this region

    [0501] Color setup: Non-similar, weakly similar: dark grey, conserved: light grey, blocks of similar: medium grey, identical: white

    [0502] FIG. 3 Phylogenetic tree based on PDCT protein sequences.

    [0503] Legend: At: Arabidopsis thaliana, Bn: Brassica napus, Bc: Brassica carinata, Cs: Camelina sativa, Gm: Glycine max, Lu: Linum usitatissimum, Rc: Ricinus communis, Ta: Triticum aestivum, Zm: Zea mays.

    [0504] * activity demonstrated in other studies

    [0505] ** proteins selected based on homology in BLAST searches of NCBI databases, activity not demonstrated

    [0506] FIG. 4. Pathway and genes in fatty acid synthesis pathway in transgenic Arabidopsis plants.

    [0507] FIG. 5. Action of PDCT (Modified from Lu et al., 2009)

    [0508] FIG. 6: Phylogenetic tree based on PDCT protein sequences of Table 5

    [0509] FIG. 7 decribes the formulas to calculate pathway step conversion efficiencies. S: substrate of pathway step.

    [0510] P: product of pathway step. Product was always the sum of the immediate product of the conversion at this pathway step, and all downstream products that passed this pathway step in order to be formed. E.g. DHA (22:6n-3 does possess a double bond that was a result of the delta-12-desaturation of oleic acid (18:1n-9) to linoleic acid (18:2n-6).

    [0511] FIG. 8:

    [0512] Needle Matrix of PCDT sequences of table 5

    [0513] FIG. 9:

    [0514] Conversion rate efficiencies of desaturases.

    EXAMPLES

    Example 1

    Materials and Methods

    [0515] Cloning of Genes:

    [0516] RNA from young root tissue of B.napus, B. carinata and C. sativa was reversed transcribed using Superscript III. Primers for cloning cDNAs were based on genomic sequence information from NCBI sequence databases (https://www.ncbi.nlm.nih.gov/) and naming of genes followed the information in these databases. The proofreading enzyme Phusion was used to clone cDNAs, which were transformed into pYes 2.1 prior to sequencing. Seven PDCT like genes were cloned from B. napus, originating from chromosome 1A, 1C, 2C, 3A, 3C, 5A and 5C. Seven genes were cloned from B. carinata, originating from chromosomes 1B, 1C, 2B, 3B,3C, 5B and 5C. Three genes were cloned from C. sativa, originating from chromosomes 1, 15 and 19. Sequences of cDNAs and translation products are given in Table 1.

    [0517] Sequence Analysis:

    [0518] All clones were sequenced prior to transformation. The protein alignment and phylogenetic tree were constructed using the software program Vector NTI.

    [0519] Construction of transformation vectors and Arabidopsis transformation:

    [0520] Because the C genome genes from B. carinata and B. napus were identical or nearly identical, only C subgenome derived PDCT genes from B. carinata were used in further experiments. PDCT genes were cloned into the pUC-19 Napin-B vector to add the Napin promotor and OSC terminator, as described in Wu et al (2005). The genes including promotors and terminators were removed by restriction enzyme digestion and ligated to pUC19-ABC carrying the Thraustocytrium sp. delta 6 elongase (Sequence ID: KH273553.1) and the P. irregulare delta 6 desaturase (Sequence ID: AF419296.1). The three genes were removed from the vector by restriction enzyme digestion and ligated into the plant binary vector pSUN2-ASC. All vectors were analyzed by restriction digestion before transformation. Controls included an empty vector and a vector containing only the P. irregulare D6 desaturase and the PSE (tc) elongase. The Arabidopsis rod1 (At3g15820) mutant line (Lu et al. 2009), kindly provided by Chaofu Lu, was used as the Arabidopsis host plant. This mutant has a G to A mutation resulting in a premature stop codon in the phosphatidylcholine:diacylglycerol cholinephosphotransferase (PDCT) enzyme encoded by the Arabidopsis ROD1 gene (Lu et al. 2009). Four plants were tested by sequencing, which indicated all were homozygous for the relevant mutation, and seed was collected from these plants and used for transformation. Plant binary vectors were transformed into Agrobacterium tumefaciens strain GV3101-pMP90. The host plant was grown until the bolting stage and transformed using the floral dip method (Clough and Bent, 1998). Essentially, Agrobacterium tumefaciens carrying each vector was grown to mid-log stage, spun down and suspended to an OD600 of 0.8 in 5% sucrose solution containing 0.05% Silwet L-77, and plants were immersed in this solution for 2-3 minutes with gentle agitation. After maturity, seeds were sterilized and germinated on ½X MS selective medium containing 50 mg/L kanamycin for selection of transgenic plants. Positive plants were transplanted into soil and grown to maturity.

    [0521] GC Analysis:

    [0522] Twenty T2 seeds from positive T1 plants were used to extract fatty acids. Seeds were placed in a clean glass tube, 2 mL of 3M methanolic HCL was added to each tube, and capped tubes were incubated at 80° C. for 4 hours. After incubation, samples were cooled to room temperature, 1 mL of 0.9% NaCl and 2 mL of hexane was then added to each sample and vortexed. Samples were then centrifuged and the hexane (top) layer was removed and added to clean glass tubes. Samples were evaporated under nitrogen until dry. 80 μL of hexane was added to the tubes and vortexed briefly to resuspend the fatty acids. The solution was then moved to a collection vial containing a GC insert, and GC analysis was performed (Table 2).

    [0523] The segregation of the transgene was tested by germinating 50-100 seeds on selective media, and testing the fit to a 3:1 hypothesis (Table 3). Seedling progeny of transgenic plants that segregated with a 3:1 ratio (consistent with expression of construct at a single locus) were used for further analysis. GC analysis of 20 seeds from 3-5 lines for each gene was conducted as described above, and fatty acid distribution was determined (Table 4).

    Example 2

    Results

    [0524] The amino acid sequences of the 19 PDCT genes cloned in this study fell in 5 distinct groups (FIGS. 1, 2 and 3). These groups consisted of the chromosome 1-derived sequences of B. napus and B. carinata, the chromosome 2 sequences of B. napus and B. carinata, the chromosome 3 sequences of B. carinata and B. napus, the chromosome 5 genes of B. napus and B. carinata and the three C. sativa sequences (FIG. 2). The amino acid translations of the C-subgenome derived genes of B. carinata and B. napus were identical or nearly identical, although there were differences in the cDNA sequences (FIG. 1, Table 1). Most of the differences in amino acid sequences occurred in the N-terminal region of the translation products, while blocks of conserved amino acids were found throughout the middle and C-terminal regions (FIG. 1). The Group 1 sequences were about 42 amino acids shorter than the other sequences in this area. The differences among the three C. sativa sequences occurred within the first 60 amino acids (FIG. 1, FIG. 2).

    [0525] The four subgenome A PDCT genes from Brassica napus, the four subgenome B and four subgenome C genes from Brassica carinata, and all three PDCT genes from Camelina sativa were co-expressed in the Arabidopsis rod1 mutant with the Δ6-desaturase from Pythium irregulare and the Δ6-elonagase from Thraustochytrium. The Arabidopsis rod1 mutant and a wild-type Arabidopsis line (with an active endogenous PDCT gene) were also transformed with the Δ9-desaturase from Pythium irregulare and the Δ6-elonagase from Thraustochytrium, and untransformed wild-type and ROD mutant lines were used for comparison.

    [0526] Expression of the Δ6-desaturase and Δ6-elonagase will result in the production of the heterologous fatty acids γ-linolenic acid (GLA ; 18:2 Δ11, 14), stearidonic acid (SDA ; 18:3 Δ6, 9, 12, 15), di-homo γ-linolenic acid (DGLA; 20:3 Δ8, 11, 14) and eicosatetraenoic acid (ETA; 20:4 Δ8.11, 14.17) in Arabidopsis seeds, as shown in FIG. 4. An active PDCT gene will lead to a decrease in the level of OA (18:1 Δ9) and an increase in the level(s) of LA (18:2Δ6, 9), ALA (18:3Δ6, 9, 15) and/or GLA, as shown in FIG. 5.

    [0527] The presence of a mutation in the ROD1 gene of Arabidopsis has been shown to increase the percent of 18:1 in seed oil (Lu et al., 2009). The percentage of 18:1 in the untransformed rod1 mutant used in this study averaged 30.42%, while seed oil of the untransformed wild-type line contained 15.334% 18:1. Seed oil from Arabidopsis lines carrying group 1 and group 2 chromosome-derived PDCT genes had average 18:1 levels ranging from 25.72-31.12% (Table 2). This was comparable to the level in the ROD mutant lines transformed with only the Δ6-desaturase and Δ6-elonagase (average 30.732%). However, the levels in seeds carrying the subgenome 3A, 3B and 3C derived genes ranged from 14.959-15.871%. Levels in seeds carrying chromosome 5 derived PDCT genes ranged from 11.994-16.696%, and those in seeds carrying the C. sativa genes ranged from 13.288-14.050%. Thus, while the Brassica napus chromosome 3 and chromosome 5 derived genes, and the three C. sativa genes are able to compensate for the mutation in the Arabidopsis PDCT gene, the chromosome 1 and 2 derived genes appear to have little or no effect on 18:1 levels. This suggests that the chromosome 1 and 2 derived genes may have a different function and/or act on different substrates than the Arabidopsis PDCT gene.

    [0528] Alignment of PDCT-like translation products from a range of species including Triticum aestivum, Arabidopsis thaliana, Zea mays, Ricinus communis, Glycine max, and Linum usitatissimum indicated that substitutions of highly conserved amino acids occurred throughout the B. napus chromosome 1 and chromosome 2 derived proteins. Using numbering based on the Arabidpsis ROD1 sequence as shown in the alignment in FIG. 1, Brassica napus chromosome-1 derived enzymes showed the following changes in conserved regions: position 102: M to T, between 104-105: insertion of E, and 225: H to Q. In addition to these changes in conserved regions, various differences occurred in the less conserved N-terminal region of the protein.

    [0529] In the case of chromosome 2B and 2C derived proteins from Brassica carinata and Brassica napus respectively, a larger number of substitutions in conserved regions were detected. Using amino acid residue numbering based on the Arabidopsis ROD1 sequence, the following substitutions were detected 98: V/L to F, 101 F to V, 102 M to V, 106: Y to S, 141: L/V to G, 149-150: FV to LG, 158: L/V to A, 176: M to V, 186: S/A to C, 192: P to S, 211: L to Y, and 230: M/V to T. Notably, this threonine substitution at position 230 also occurred in most of the chromosome 1 group proteins, as did the M to T substitution at position 106.

    [0530] In the untransformed Arabidopsis wild-type lines the decrease in 18:1 is compensated for by an increase in 18:2 compared to rod1 mutant plants (27. 545% in wild-type versus 14.323% in ROD mutant; Table 2) although a slight increase in ALA also occurs (16.066 versus 14.323%). Transgenic lines carrying the elongase and desaturase genes plus chromosome—1 or 2 PDCT genes had LA levels of 8.314-12.165%, while lines carrying chromosome 3 and 5 derived PDCT genes had levels of 18.149-20.142%. The lines carrying the C. sativa genes had 18:2 levels of 11.324% (Chromosome 1 derived PDCT), 19.912% (C15) and 8.635% (C19). ALA levels were also comparatively low in lines carrying the genes had the highest average ALA content (14.826%). However, in lines carrying the Δ6-desaturase and the Δ6-elonagase along with the PDCT gene, the additional 18:2 produced in the presence of the PDCT gene may be used not only to produce ALA, but may also be used in the synthesis of GLA, DGLA, SDA and ETA (FIG. 4). The total levels of these fatty acids were highest in lines carrying the C1 (25.225%) and C19 (24.379%) PDCT genes, and these two lines also had the highest levels of GLA plus HGLA (22.183% and 21.094% respectively). The fatty acid profile of lines carrying the C. sativa C15 gene bore more of a resemblance to the group 5 and group 3 chromosomes, in that the total ALA plus SDA plus ETA (16%) was considerably higher than the total GLA plus HGLA (8.767%). Only in the C1 and C19 lines were total levels of GLA plus HGLA higher than total levels of ALA plus SDA plus ETA (Table 2). Thus, not only do the various PDCTs show differences in overall efficiency, but there also appears to be different substrate preferences among the genes. The Camelina sativa C1 and C19 proteins differed from the C15 protein in only a limited number of amino acids in the N-terminal region of the protein (FIG. 2). Position 3 was valine in C15 and alanine in C1 and C19. Position 4 was alanine in C15, whereas the similar amino acid residues serine and threonine were at position 4 in C1 and C19 respectively. A conserved histidine at position 20 in C1 and C19 was replaced by asparagine in C15, proline-valine residues at positions 35 to 36 in C1 and C19 were replaced with arginine-isoleucine in C15, and a threonine at position 41 was replaced with lysine in C15. Finally, C15 had an insertion of an amino acid (glycine) at position 63. These differences indicated the importance of the N-terminal region of the PDCT enzyme in determining enzyme activity.

    [0531] Potentially, inactivation of one or more Camelina sativa PDCT enzyme may modulate PDCT activity levels, and might also be beneficial in increasing the levels of specific fatty acids, or in pushing fatty acids towards the ω3 or ω6 pathway. Since B. napus and B. carinata each have four active PDCT genes, it should be possible to achieve a range in PDCT activity levels by combining active and inactive genes. Avoiding rapid transfer onto DAG may allow more efficient transfer to the acyl-CoA pool by the reverse reaction of plant LPCAT enzymes. The reverse reaction of LPCAT has been shown to play an important role in editing PC in plants, and plant LPCATs also show fatty acid selectivity (Lager et al., 2013) This may be of particular interest for the production of VLC-PUFAs, where rapid movement of fatty acids to the DAG pool and subsequently to TAG may not be desirable.

    [0532] To ensure the differences in activities among the transgenic lines did not reflect differences in copy numbers of PDCT genes, the segregation ratio of T2 plants was checked (Table 3), and T3 seed from lines that fit a 3:1 segregation ratio was used for GC analysis. Results closely resembled those from the T2 generation (Table 4). 18:1 levels in lines carrying chromosome group 1 or 2 derived PDCT genes ranged from 31.26-31.41%, while levels in group 3 and 5 lines ranged from 12.17-14.59%. Levels in lines carrying the C. sativa genes ranged from 12.89 to 14.60%. LA levels in lines carrying group 1 and 2 chromosome genes ranged from 6.58-10.06%, while levels in the group of lines carrying chromosome 3 or 5 derived genes ranged from 15.58-23.54%. Levels in lines carrying C1, C15 and C19 PDCT genes were 11.53, 21.49 and 7.50%, respectively. Again, the low level of LA in C1 and C19 lines was due to the very high levels of GLA plus DGLA in these lines (20.85% in C1 and 23.11% in C19).

    Example 3

    Average Fatty Acid Composition (%) in Different Lipid Classes from Immature Seeds

    [0533] Thin-layer chromatography (TLC) analysis was performed on immature siliques (from plants homozygous for the desaturase and elongase transgenes) to measure the fatty acid profile in different lipid pools, namely, phosphatyidylcholine (PC), diacylglycerol (DAG), and triacylglycerol (TAG). Briefly, total lipids were extracted from immature siliques by rapid freezing and grinding of green siliques, followed by transferring approximately 500 mg of ground sample into a centrifuge tube with 3 ml of chloroform: methanol: formic acid (10:10:1, v/v/v) and storing overnight at −20° C. After centrifugation, the supernatant was collected, and the pellet was re-extracted with 1.1 ml chloroform: methanol: water (5:5:1, v/v/v). The extractions were combined and washed with 1.5 ml mL 0.2M H3PO4/1M KCl. Lipids in the chloroform phase were dried down, and re-dissolved in 0.2 ml of chloroform. After pre-running and drying the TLC plate, samples were run in hexane/diethyl ether/acetic acid (70:30:1). TAG and DAG were isolated and directly methylated with 3M methanolic HCL. Polar lipids were collected from the plate, extracted and resuspended in chloroform, then re-run in chloroform/methanol/acetic acid/water (60:30:3:1) to separate PC. Bands were visualized by spraying with primulin solution and exposing to UV light. The appropriate silica bands were scraped from the TLC plate, and treated with 2 mL 3M methanolic HCL at 80° C., then analyzed by GC. All fatty acid data are presented as % relative and are shown in Table 7.

    [0534] The data in Table 7 can be used to understand how the PDCT genes influence the trafficking of fatty acids between different lipid pools. Table 6 shows the average fatty acid composition (%) in different lipid classes from immature seeds of Arabidopsis transformed with D6(Pi) desaturase+ Tc D6Elongase.

    [0535] The Arabidopsis rod1 mutant (CK mutant) and a wild-type Arabidopsis line (CK WT) (with an active endogenous PDCT gene) were also transformed with the Δ6-desaturase from Pythium irregulare and the Δ6-elonagase from Thraustochytrium, and untransformed wild-type (WT) and ROD mutant lines (Rod mut) were used for comparison.

    TABLE-US-00006 TABLE 1 Sequenzes: PDCT1 Polypeptide: SEQ ID No.: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46 PDCT1 Polynucleotide: SEQ ID No.: 1, 3, 5, 7, 9, 11, 13, 15, 39, 41, 43, and/or 45 PDCT19 Polypeptide: SEQ ID No.: 36, 38, and/or 48 PDCT19 Polynucleotide: SEQ ID No.: 35, 37, and/or 47 PDCT3/5 Polypeptide: SEQ ID No.: 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60 PDCT19 Polynucleotide: SEQ ID No.: 17, 19, 21, 23, 27, 29, 31, 49, 51, 53, 55, and/or 57 Candiates of the PDCT1 that shall have the same activity as PDCT1: GmROD1-1 63 64 PDCT1 candiate GmROD1-2 65 66 PDCT1 candiate RcPDCT 67 68 PDCT1 candiate RcROD1_SEQIDNO9 69 70 PDCT1 candiate LuPDCT1 71 72 PDCT1 candiate LuPDCT2 73 74 PDCT1 candiate

    TABLE-US-00007 TABLE 2 Average fatty acid composition (%) in seeds of PDCT + D6(Pi) desaturase + Tc D6Elongase transgenic T2 Arabidopsis. Total GLA Total HGLA ALA Total SDA SDA GLA 16:0 18:0 18:1 18:2 GLA 18:3 20:1 HGLA SDA ETA ETA ETA HGLA Napus 9.063 3.290 26.438 12.165 2.205 9.916 15.452 5.634 0.231 0.995 9.065 11.142 7.893 1A Carinata 8.254 3.398 29.693 11.760 2.402 10.656 17.882 1.950 0.689 0.369 5.410 11.714 4.352 1C Carinata 7.950 3.203 30.947 11.418 3.083 11.154 18.672 1.639 0.742 0.253 5.717 12.149 4.722 2B Napus 8.045 3.239 29.547 11.834 3.903 10.586 17.472 1.703 0.898 0.237 6.741 11.721 5.606 2C Napus 7.915 3.004 15.871 18.613 7.984 12.877 17.168 1.827 1.226 0.090 11.127 14.193 9.811 3A Carinata 7.756 3.027 15.287 18.974 7.977 13.180 17.220 1.777 1.282 0.053 11.089 14.515 9.755 3B Carinata 7.846 3.495 14.959 17.639 8.662 13.744 18.638 2.096 1.374 0.215 12.347 15.333 10.758 3C Napus 7.606 3.286 16.696 18.657 6.467 14.627 18.890 1.192 1.092 0.065 8.816 15.784 7.659 5A Carinata 8.031 3.244 15.025 18.149 9.193 12.762 16.790 2.481 1.493 0.155 13.322 14.410 11.674 5B Carinata 8.429 2.905 11.994 20.812 9.901 11.717 15.036 2.453 1.653 0.102 13.821 13.184 12.354 5C C1 9.126 3.440 13.288 11.324 14.380 7.771 15.141 7.803 2.063 0.980 25.225 10.813 22.183 (80666) C15 8.196 3.489 14.367 19.912 7.366 14.826 18.397 1.401 1.158 0.016 9.941 16.000 8.767 (45897) C19 7.830 3.454 14.050 8.635 14.658 7.656 15.746 6.436 2.440 0.844 24.379 10.940 21.094 (65416) CK 8.936 3.290 30.732 11.866 3.172 11.105 17.449 1.905 0.602 0.198 5.877 11.905 5.077 mutant CK WT 7.684 3.345 12.754 22.068 7.527 13.765 18.000 1.906 0.968 0.143 10.544 14.876 9.433 WT 7.335 3.284 15.334 27.545 0.000 16.066 18.071 0.000 0.000 0.000 0.000 16.066 0.000 ROD 7.619 3.123 30.420 14.332 0.000 15.158 19.276 0.000 0.000 0.000 0.000 15.158 0.000 mut CK mutant: PDCT mutant with D6(Pi) desaturase + Tc D6Elongase CK WT: WT Arabidopsis with D6(Pi) desaturase + Tc D6Elongase WT: Untransformed wild-type Arabidopsis ROD mut: Untransformed Arabidopsis ROD mutant Complete data in Appendix 1.

    TABLE-US-00008 TABLE 3 Segregation ratios of T2 generation to test goodness of fit to 3:1 ratio Plant Resistant Susceptible Hypothesis Accept Group # plant plant Ratio p value hypothesis B. napus 1A  2 50 0 63:1  0.312 Accept  4 12 9 3:1 0.04 No  5 71 19 3:1 0.46 Accept  6 61 11 3:1 0.06 Accept  7 20 45 3:1 0.249 Accept  8 40 13 3:1 1 Accept  9 41 26 3:1 0.012 No 10 38 16 3:1 0.34 Accept 11 40 16 3:1 0.537 Accept 12 65 20 3:1 0.801 Accept 13 67 18 3:1 0.451 Accept 14 32 15 3:1 0.316 Accept 15 50 9 3:1 0.073 Accept 16 103 23 3:1 0.1 Accept 17 54 19 3:1 0.786 Accept 18 35 64 1:3 0.021 No 19 54 18 3:1 1 Accept 20 74 14 3:1 0.049 No 21 22 8 3:1 1 Accept 22 83 23 3:1 0.498 Accept 23 52 17 3:1 1 Accept 24 73 16 3:1 0.14 Accept Accept Plant Resistant Susceptible Hypothesis hypothesis Group # plant plant Ratio p value or not B. carinata 2B  1 72 20 3:1 0.47 Accept  2 59 19 3:1 1 Accept  3 73 23 3:1 0.814 Accept  4 45 15 3:1 1 Accept  5 99 5 15:1  0.674 Accept  6 75 9 15:1  0.065 Accept  7 103 11 15:1  0.119 Accept  8 107 16 3:1 0.001 No  9 98 12 15:1  0.051 Accept 10 119 5 15:1  0.273 Accept 12 50 0 63:1  0.312 Accept 13 136 16 15:1  0.016 No 14 113 19 3:1 0.005 No 15 142 11 15:1  0.744 Accept 16 50 5 15:1  0.235 Accept 17 84 11 15:1  0.035 No 18 88 29 3:1 1 Accept 19 107 9 15:1  0.435 Accept 20 105 10 15:1  0.242 Accept 21 101 25 3:1 0.215 Accept 22 76 3 15:1  0.355 Accept 23 65 16 3:1 0.302 Accept 24 51 21 3:1 0.414 Accept 25 51 16 3:1 0.779 Accept B. napus 2C  1 95 20 3:1 0.053 Accept  2 55 17 3:1 0.785 Accept  3 50 2 15:1  0.552 Accept  4 120 12 15:1  0.145 Accept  5 130 16 15:1  0.016 No  6 160 14 15:1  0.35 Accept  7 103 10 15:1  0.242 Accept  8 60 19 3:1 0.796 Accept  9 74 26 3:1 0.817 Accept 10 58 25 3:1 0.313 Accept 11 118 12 15:1  0.144 Accept 12 98 2 63:1  1 Accept 13 42 24 3:1 0.027 No 14 71 1 63:1  1 Accept 15 75 25 3:1 1 Accept 16 38 29 3:1 0.001 No 17 125 21 3:1 0.004 No 18 143 35 3:1 0.118 Accept 19 107 2 63:1  1 Accept 20 81 23 3:1 0.497 Accept 21 60 1 63:1  1 Accept 22 92 1 63:1  1 Accept Plant Resistant Susceptible Hypothesis Accept Group # plant plant Ratio p value hypothesis B. napus 3A  1 67 12 3:1 0.038 No  2 125 39 3:1 0.718 Accept  3 29 26 3:1 0 No  4 92 21 3:1 0.127 Accept  5 67 20 3:1 0.622 Accept  6 43 19 3:1 0.342 Accept  7 55 26 3:1 0.236 Accept  8 70 9 15:1  0.065 Accept  9 60 7 15:1  0.122 Accept 10 63 5 15:1  0.606 Accept 11 60 18 3:1 0.792 Accept 12 68 22 3:1 1 Accept 13 56 29 3:1 0.044 No 14 69 22 3:1 0.809 Accept 15 70 2 63:1  0.314 Accept 16 41 13 3:1 1 Accept 17 53 3 15:1  1 Accept 18 47 16 3:1 1 Accept 19 77 13 3:1 0.027 No 20 78 6 15:1  0.645 Accept 21 90 15 3:1 0.013 No 22 47 11 3:1 0.357 Accept 23 35 11 3:1 1 Accept 24 61 20 3:1 1 Accept B. carinata 3B 3B-1 76 7 15:1  0.3562 Accept 3B-2 56 23 3:1 0.4376 Accept 3B-3 24 28 3:1 <0.0001 Reject 3B-4 58 15 3:1 0.415 Accept 3B-5 27 45 3:1 <0.0001 Reject 3B-6 142 37 3:1 0.168 Accept 3B-7 85 31 3:1 0.668 Accept 3B-8 87 21 3:1 0.182 Accept 3B-9 75 24 3:1 0.817 Accept 3B-10 97 11 15:1  0.118 Accept 3B-11 52 13 3:1 0.388 Accept 3B-12 43 18 3:1 0.372 Accept 3B-13 75 29 3:1 0.497 Accept 3B-14 63 3 15:1  0.606 Accept 3B-15 42 16 3:1 0.539 Accept 3B-16 70 4 15:1  0.643 Accept 3B-17 68 2 63:1  0.314 Accept 3B-18 56 23 3:1 0.438 Accept 3B-19 59 5 15:1  0.606 Accept 3B-20 71 2 63:1  0.314 Accept 3B-21 56 2 63:1  0.313 Accept 3B-22 58 22 3:1 0.606 Accept 3B-23 59 19 3:1 1 Accept 3B-24 65 30 3:1 0.157 Accept B. carinata 3C  1 128 2 63:1  1 Accept  2 96 17 3:1 0.017 No  5 78 24 3:1 0.818 Accept  6 76 8 15:1  0.167 Accept  7 50 5 15:1  0.235 Accept  8 91 15 3:1 0.013 No  9 75 10 15:1  0.021 No 10 95 17 3:1 0.016 No 11 97 13 15:1  0.019 No 12 38 12 3:1 1 Accept 13 80 10 15:1  0.091 Accept 14 42 6 15:1  0.074 Accept 15 77 17 3:1 0.15 Accept 16 70 5 15:1  1 Accept 17 120 1 63:1  0.476 Accept 18 79 24 3:1 0.65 Accept 19 61 15 3:1 0.289 Accept 20 94 20 3:1 0.082 Accept 21 59 13 3:1 0.174 Accept 22 109 15 15:1  0.011 No 23 49 19 3:1 0.575 Accept 25 53 17 3:1 1 Accept 34 65 22 3:1 1 Accept 39 77 23 3:1 0.644 Accept 24 58 1 63:1  1 Accept B. napus 5A 5A-1 32 17 3:1 0.097 Accept 5A-3 53 17 3:1 1 Accept 5A-4 49 14 3:1 0.563 Accept 5A-5 50 21 3:1 0.413 Accept 5A-6 36 13 3:1 0.74 Accept 5A-8 70 6 15:1  0.644 Accept 5A-9 35 11 3:1 1 Accept 5A--10 32 15 3:1 0.316 Accept 5A-11 47 7 15:1  0.017 Reject 5A-12 71 1 63:1  1 Accept 5A-13 52 15 3:1 0.574 Accept 5A-14 45 17 3:1 0.553 Accept 5A-15 61 28 3:1 0.14 Accept 5A-16 61 24 3:1 0.451 Accept 5A-17 78 25 3:1 0.821 Accept 5A-18 56 24 3:1 0.302 Accept 5A-19 46 14 3:1 0.766 Accept 5A-20 60 19 3:1 0.796 Accept 5A-21 86 14 3:1 0.011 Reject 5A-23 54 9 15:1  0.01 Reject 5A-25 48 17 3:1 0.773 Accept 5A-26 53 18 3:1 1 Accept 5A-1 32 17 3:1 0.097 Accept 5A-3 53 17 3:1 1 Accept 5A-4 49 14 3:1 0.563 Accept 5A-5 50 21 3:1 0.413 Accept B. carinata 5B 5B-1 54 3 15:1  0.6041 Accept 5B-2 49 15 3:1 0.7728 Accept 5B-3 50 12 3:1 0.3737 Accept 5B-4 59 20 3:1 1 Accept 5B-5 76 29 3:1 0.4976 Accept 5B-6 58 12 3:1 0.1634 Accept 5B-7 68 21 3:1 0.806 Accept 5B-8 67 22 3:1 1 Accept 5B-9 74 18 3:1 0.229 Accept 5B-10 112 26 3:1 0.114 Accept 5B-11 48 20 3:1 0.401 Accept 5B-12 53 21 3:1 0.416 Accept 5B-13 57 24 3:1 0.303 Accept 5B-14 63 16 3:1 0.301 Accept 5B-15 107 9 15:1  0.435 Accept 5B-16 99 32 3:1 0.84 Accept 5B-17 56 14 3:1 0.403 Accept 5B-18 56 19 3:1 1 Accept 5B-19 42 23 3:1 0.044 Reject 5B-20 125 7 15:1  0.715 Accept 5B-21 26 29 3:1 <0.0001 Reject 5B-22 33 11 3:1 1 Accept 5B-23 51 19 3:1 0.784 Accept B. carinata 5C 5C-18 118 42 3:1 0.715 Yes 5C-11 76 26 3:1 0.8179 Yes 5C-15 114 101 3:1 0.0001 No 5C-12 70 16 3:1 0.2095 Yes 5C-2 52 17 3:1 1 Yes 5C-10 79 3 15:1  0.356 Yes 5C-26 88 13 3:1 0.0057 No 5C-20 59 23 3:1 0.4404 Yes 5C-25 60 16 3:1 0.4268 Yes 5C-5 66 14 3:1 0.1213 Yes 5C-19 45 6 3:1 0.0245 No 5C-6 95 3 15:1  0.2062 Yes 5C-16 93 94 3:1 0.0001 No 5C-9 112 7 15:1  1 Yes 5C-17 116 37 3:1 0.8516 Yes 5C-8 156 58 3:1 0.529 Yes 5C-13 72 43 3:1 0.0026 No 5C-1 72 27 3:1 0.4817 Yes 5C-7 140 124 3:1 0.0001 No 5C-14 41 24 3:1 0.0213 No 5C-3 64 33 3:1 0.0342 No Plant Hypothesis Accept Group # Resistant Susceptible Ratio p value hypothesis C. sativa C1 80666-15 54 8 3:1 0.0684 Yes 80666-20 50 12 3:1 0.3737 Yes 80666-17 52 20 3:1 0.5862 Yes 80666-13 48 18 3:1 0.5657 Yes 80666-1 24 29 3:1 0.0001 No 80666-3 39 32 3:1 0.0001 No 80666-16 45 17 3:1 0.5531 Yes 80666-19 55 18 3:1 1 Yes C. sativa C15 45897-16 68 17 3:1 0.3144 Yes 45897-5 60 20 3:1 1 Yes 45897-18 63 18 3:1 0.6063 Yes 45897-8 82 6 15:1  0.6452 Yes 45897-14 51 16 3:1 0.7789 Yes 45897-1 53 8 3:1 0.0374 No 45897-15 66 4 15:1  1 Yes 45897-9 55 17 3:1 0.7855 Yes 45897-13 81 19 3:1 0.1659 Yes 45897-12 58 20 3:1 0.7919 Yes 45897-11 58 30 3:1 0.0489 No 45897-10 59 15 3:1 0.4163 Yes 45897-6 58 17 3:1 0.5954 Yes 45897-7 63 21 3:1 1 Yes 45897-17 53 16 3:1 0.78 Yes 45897-19 57 17 3:1 0.7864 Yes 45897-2 56 11 3:1 0.0921 Yes 45897-20 65 19 3:1 0.6143 Yes 45897-3 64 1 63:1  1 Yes 45897-4 63 15 3:1 0.2914 Yes C. sativa C19 65416-1 97 34 3:1 0.84 Accept 65416-2 59 22 3:1 0.61 Accept 65416-3 81 37 3:1 0.09 Accept 65416-4 69 45 3:1 0.0002 No 65416-5 174 47 3:1 0.213 Accept 65416-6 176 37 3:1 0.01 No 65416-7 99 19 3:1 0.03 No 65416-8 123 26 3:1 0.04 No 65416-9 110 18 15:1  0.0002 No 65416-10 153 14 15:1  0.192 Accept 65416-11 97 35 3:1 0.688 Accept 65416-12 102 7 15:1  1 Accept 65416-13 92 33 3:1 0.679 Accept 65416-14 113 71 3:1 0 No 65416-15 120 48 3:1 0.285 Accept 65416-16 106 60 3:1 0.0006 No 65416-17 203 63 3:1 0.67 Accept 65416-19 165 52 3:1 0.75 Accept 65416-20 40 11 3:1 0.52 Accept 65416-21 261 78 3:1 0.38 Accept

    TABLE-US-00009 TABLE 4 Average fatty acid composition (%) in transgenic T3 plants. Complete data in Appendix 2. LINE 16:0 18:0 18:1 18:2 GLA 18:3 20:1 1A 8.96 ± 0.52 3.69 ± 0.15 31.6 ± 1.49 6.58 ± 0.99 3.40 ± 0.25 6.33 ± 0.54 17.0 ± 0.87 1C 8.38 ± 0.18 3.65 ± 0.09 32.3 ± 0.59 11.9 ± 3.22 3.11 ± 0.69 9.50 ± 1.22 18.6 ± 0.44 2B 8.44 ± 0.61 3.54 ± 0.15 30.3 ± 2.20 10.0 ± 2.27 4.07 ± 0.81 8.44 ± 2.00 17.7 ± 1.04 2C 8.36 ± 0.41 3.58 ± 0.22 31.1 ± 1.70 8.79 ± 2.63 3.80 ± 0.62 8.22 ± 2.67 18.1 ± 0.92 3A 8.25 ± 0.85 3.59 ± 0.54 13.8 ± 3.03 19.5 ± 1.92 8.00 ± 1.21 12.1 ± 1.50 17.8 ± 1.32 3B 8.69 ± 0.32 3.25 ± 0.07 12.5 ± 1.82 18.4 ± 3.35 11.5 ± 2.99 12.2 ± 2.02 16.4 ± 0.82 3C 8.13 ± 0.27 3.59 ± 0.07 14.3 ± 1.09 15.5 ± 4.40 11.5 ± 3.97 10.1 ± 2.36 17.7 ± 0.82 5A 8.33 ± 0.12 3.10 ± 0.12 12.1 ± 0.79 23.5 ± 6.02 8.90 ± 1.49 13.6 ± 0.59 16.4 ± 0.27 5B 8.86 ± 0.36 3.28 ± 0.17 14.6 ± 8.96 15.6 ± 4.73 12.7 ± 1.84 10.6 ± 2.29 16.0 ± 1.13 5C 7.73 ± 0.24 2.95 ± 0.07 14.5 ± 1.10 22.0 ± 0.99 6.90 ± 1.20 13.8 ± 0.87 18.0 ± 0.43 C1 7.82 ± 0.13 3.27 ± 0.15 12.8 ± 2.12 11.5 ± 7.13 15.0 ± 7.82 9.42 ± 3.98 17.2 ± 0.42 C15 7.96 ± 0.06 2.96 ± 0.06 13.3 ± 0.73 21.4 ± 0.44 8.29 ± 0.74 12.9 ± 0.43 17.4 ± 0.09 C19 7.70 ± 0.24 3.71 ± 0.17 14.6 ± 1.62 7.50 ± 0.89 16.9 ± 2.36 7.81 ± 0.74 16.9 ± 0.44 GLA DGLA ALA SDA SDA GLA LINE DGLA SDA ETA ETA ETA DGLA 1A 7.49 ± 0.85 0.55 ± 0.23 1.40 ± 0.22 12.83 8.27 10.89 1C 2.03 ± 0.92 0.91 ± 0.22 0.38 ± 0.26 6.43 10.78 5.15 2B 3.32 ± 2.22 0.79 ± 0.15 0.43 ± 0.37 8.61 9.66 7.39 2C 3.69 ± 2.99 0.80 ± 0.13 0.66 ± 0.56 8.96 9.69 7.50 3A 1.56 ± 0.63 1.09 ± 0.20 0.00 10.64 13.27 9.55 3B 1.25 ± 0.74 1.77 ± 0.92 0.00 14.60 14.05 12.83 3C 3.33 ± 3.08 1.63 ± 0.67 0.28 ± 0.48 16.77 12.02 14.86 5A 1.68 ± 0.69 1.18 ± 0.2  0.00 11.77 14.83 10.58 5B 1.71 ± 1.53 1.85 ± 0.31 0.01 ± 0.01 16.26 12.53 14.41 5C 0.87 ± 0.49 0.94 ± 0.18 0.00 8.70 14.79 7.76 C1 5.79 ± 3.13 2.40 ± 1.26 0.76 ± 0.51 24.02 12.58 20.85 C15 1.49 ± 0.39 1.11 ± 0.13 0.02 ± 0.04 10.91 14.07 9.78 C19 6.12 ± 0.67 2.57 ± 0.46 0.87 ± 0.14 26.55 11.25 23.11

    TABLE-US-00010 TABLE 5 SEQ SEQ ID ID PDCT Name NA AA Activity Organism Napus_1A 1 2 PDCT1 Brasssica napus Napus_2A 3 4 PDCT1 Brasssica napus Carinata_1B 5 6 PDCT1 Brassica carinata Carinata_1C 7 8 PDCT1 Brassica carinata Carinata_2B 9 10 PDCT1 Brassica carinata Carinata_2C 11 12 PDCT1 Brassica carinata BjROD1-B4 13 14 PDCT1 Brassica juncea BjROD1-A3 15 16 PDCT1 Brassica juncea BjROD1-B3 39 40 PDCT1 Brassica juncea Napus_1C 41 42 PDCT1 Brasssica napus Napus_2C 43 44 PDCT1 Brasssica napus Consensus PDCT1 45 46 PDCT1 Artificial Napus_3A 17 18 PDCT3/5 Brasssica napus Napus_5A 19 20 PDCT3/5 Brasssica napus Carinata_3B 21 22 PDCT3/5 Brassica carinata Carinata_3C 23 24 PDCT3/5 Brassica carinata Carinata_5B 25 26 PDCT3/5 Brassica carinata Carinata_5C 27 28 PDCT3/5 Brassica carinata BjROD1-A2 29 30 PDCT3/5 Brassica juncea BjROD1-B2 31 32 PDCT3/5 Brassica juncea BjROD1-B1 49 50 PDCT3/5 Brassica juncea BjROD1-A1 51 52 PDCT3/5 Brassica juncea BrROD1_SEQIDNO7 53 54 PDCT3/5 Brassica rapa Napus_5C 55 56 PDCT3/5 Brasssica napus Napus_3C 57 58 PDCT3/5 Brasssica napus Consensus PDCT3/5 59 60 PDCT3/5 Artificial Camelina_C15(45897) 33 34 PDCT15 Camelina sativa Camelina_C19(65416) 35 36 PDCT19 Camelina sativa Camelina_C1(80666) 37 38 PDCT19 Camelina sativa Consensus PDCT19 47 48 PCDT19 Artificial AtRodD1 61 62 Arabidopsis thaliana GmROD1-1 63 64 PDCT1 Glycine max candiate GmROD1-2 65 66 PDCT1 Glycine max candiate RcPDCT 67 68 PDCT1 Ricinis candiate communis RcROD1_SEQIDNO9 69 70 PDCT1 Ricinis candiate communis LuPDCT1 71 72 PDCT1 Linum candiate usitatissimum LuPDCT2 73 74 PDCT1 Linum candiate usitatissimum OsROD1_SEQIDNO11 75 76 / Oryza sativa ZmROD1_GRMZM2G015040 77 78 / Zea mays

    TABLE-US-00011 TABLE 6 Needle Protein Identity % Default Seq_1 Seq_2 settings ATRODD1 ATRODD1 100 ATRODD1 BJROD1-A1 78.8 ATRODD1 BJROD1-A2 76.1 ATRODD1 BJROD1-A3 72.7 ATRODD1 BJROD1-B1 78.5 ATRODD1 BJROD1-B2 78.1 ATRODD1 BJROD1-B3 73.7 ATRODD1 BJROD1-B4 55.5 ATRODD1 BRROD1_SEQIDNO7 78.8 ATRODD1 CAMELINA_C1(80666) 86.1 ATRODD1 CAMELINA_C15(45897) 85.8 ATRODD1 CAMELINA_C19(65416) 86.2 ATRODD1 CARINATA_1B 73.7 ATRODD1 CARINATA_1C 74 ATRODD1 CARINATA_2B 55.5 ATRODD1 CARINATA_2C 55.5 ATRODD1 CARINATA_3B 78.5 ATRODD1 CARINATA_3C 78.8 ATRODD1 CARINATA_5B 80.5 ATRODD1 CARINATA_5C 79.8 ATRODD1 GMROD1-1 60.7 ATRODD1 GMROD1-2 58.1 ATRODD1 LUPDCT1 54.6 ATRODD1 LUPDCT2 54.2 ATRODD1 NAPUS_1A 72.7 ATRODD1 NAPUS_1C 73.7 ATRODD1 NAPUS_2A 55.5 ATRODD1 NAPUS_2C 55.1 ATRODD1 NAPUS_3A 79.2 ATRODD1 NAPUS_3C 78.8 ATRODD1 NAPUS_5A 79.7 ATRODD1 NAPUS_5C 80.1 ATRODD1 OSROD1_SEQIDNO11 45.5 ATRODD1 RCPDCT 58.7 ATRODD1 RCROD1_SEQIDNO9 58.7 ATRODD1 ZMROD1_GRMZM2G015040 44.4 ATRODD1 ZMROD1_GRMZM2G087896 42.9 BJROD1-A1 ATRODD1 78.8 BJROD1-A1 BJROD1-A1 100 BJROD1-A1 BJROD1-A2 82.6 BJROD1-A1 BJROD1-A3 77.8 BJROD1-A1 BJROD1-B1 96.8 BJROD1-A1 BJROD1-B2 83.7 BJROD1-A1 BJROD1-B3 77.8 BJROD1-A1 BJROD1-B4 57.1 BJROD1-A1 BRROD1_SEQIDNO7 99.3 BJROD1-A1 CAMELINA_C1(80666) 76.8 BJROD1-A1 CAMELINA_C15(45897) 76.5 BJROD1-A1 CAMELINA_C19(65416) 76.5 BJROD1-A1 CARINATA_1B 77.8 BJROD1-A1 CARINATA_1C 78.8 BJROD1-A1 CARINATA_2B 57.1 BJROD1-A1 CARINATA_2C 57.1 BJROD1-A1 CARINATA_3B 96.8 BJROD1-A1 CARINATA_3C 97.9 BJROD1-A1 CARINATA_5B 87.1 BJROD1-A1 CARINATA_5C 86.5 BJROD1-A1 GMROD1-1 62.4 BJROD1-A1 GMROD1-2 62.8 BJROD1-A1 LUPDCT1 54.5 BJROD1-A1 LUPDCT2 54.5 BJROD1-A1 NAPUS_1A 77.1 BJROD1-A1 NAPUS_1C 78.5 BJROD1-A1 NAPUS_2A 57.1 BJROD1-A1 NAPUS_2C 56.8 BJROD1-A1 NAPUS_3A 98.2 BJROD1-A1 NAPUS_3C 97.9 BJROD1-A1 NAPUS_5A 86.5 BJROD1-A1 NAPUS_5C 86.8 BJROD1-A1 OSROD1_SEQIDNO11 45.3 BJROD1-A1 RCPDCT 58.6 BJROD1-A1 RCROD1_SEQIDNO9 58.6 BJROD1-A1 ZMROD1_GRMZM2G015040 45.3 BJROD1-A1 ZMROD1_GRMZM2G087896 44.1 BJROD1-A2 ATRODD1 76.1 BJROD1-A2 BJROD1-A1 82.6 BJROD1-A2 BJROD1-A2 100 BJROD1-A2 BJROD1-A3 77.2 BJROD1-A2 BJROD1-B1 83.3 BJROD1-A2 BJROD1-B2 87 BJROD1-A2 BJROD1-B3 77.9 BJROD1-A2 BJROD1-B4 55.4 BJROD1-A2 BRROD1_SEQIDNO7 83 BJROD1-A2 CAMELINA_C1(80666) 71.7 BJROD1-A2 CAMELINA_C15(45897) 71.9 BJROD1-A2 CAMELINA_C19(65416) 72.5 BJROD1-A2 CARINATA_1B 77.9 BJROD1-A2 CARINATA_1C 78.3 BJROD1-A2 CARINATA_2B 55.4 BJROD1-A2 CARINATA_2C 55.4 BJROD1-A2 CARINATA_3B 83.3 BJROD1-A2 CARINATA_3C 83 BJROD1-A2 CARINATA_5B 88.8 BJROD1-A2 CARINATA_5C 93.3 BJROD1-A2 GMROD1-1 62.1 BJROD1-A2 GMROD1-2 57.5 BJROD1-A2 LUPDCT1 51.5 BJROD1-A2 LUPDCT2 51.5 BJROD1-A2 NAPUS_1A 76.6 BJROD1-A2 NAPUS_1C 77.9 BJROD1-A2 NAPUS_2A 55.4 BJROD1-A2 NAPUS_2C 55.1 BJROD1-A2 NAPUS_3A 81.7 BJROD1-A2 NAPUS_3C 83 BJROD1-A2 NAPUS_5A 95.4 BJROD1-A2 NAPUS_5C 93.6 BJROD1-A2 OSROD1_SEQIDNO11 42.2 BJROD1-A2 RCPDCT 59.7 BJROD1-A2 RCROD1_SEQIDNO9 59.7 BJROD1-A2 ZMROD1_GRMZM2G015040 45.1 BJROD1-A2 ZMROD1_GRMZM2G087896 45.6 BJROD1-A3 ATRODD1 72.7 BJROD1-A3 BJROD1-A1 77.8 BJROD1-A3 BJROD1-A2 77.2 BJROD1-A3 BJROD1-A3 100 BJROD1-A3 BJROD1-B1 78.5 BJROD1-A3 BJROD1-B2 76.1 BJROD1-A3 BJROD1-B3 95.8 BJROD1-A3 BJROD1-B4 55.4 BJROD1-A3 BRROD1_SEQIDNO7 78.5 BJROD1-A3 CAMELINA_C1(80666) 69.1 BJROD1-A3 CAMELINA_C15(45897) 69.5 BJROD1-A3 CAMELINA_C19(65416) 69.5 BJROD1-A3 CARINATA_1B 95.8 BJROD1-A3 CARINATA_1C 94.5 BJROD1-A3 CARINATA_2B 55 BJROD1-A3 CARINATA_2C 55.4 BJROD1-A3 CARINATA_3B 78.8 BJROD1-A3 CARINATA_3C 78.8 BJROD1-A3 CARINATA_5B 79.3 BJROD1-A3 CARINATA_5C 78.2 BJROD1-A3 GMROD1-1 60.2 BJROD1-A3 GMROD1-2 54.4 BJROD1-A3 LUPDCT1 52.5 BJROD1-A3 LUPDCT2 53.4 BJROD1-A3 NAPUS_1A 98.6 BJROD1-A3 NAPUS_1C 95.5 BJROD1-A3 NAPUS_2A 55.4 BJROD1-A3 NAPUS_2C 55 BJROD1-A3 NAPUS_3A 78.3 BJROD1-A3 NAPUS_3C 78.8 BJROD1-A3 NAPUS_5A 78.2 BJROD1-A3 NAPUS_5C 78.5 BJROD1-A3 OSROD1_SEQIDNO11 41.8 BJROD1-A3 RCPDCT 57 BJROD1-A3 RCROD1_SEQIDNO9 57 BJROD1-A3 ZMROD1_GRMZM2G015040 43.7 BJROD1-A3 ZMROD1_GRMZM2G087896 42.7 BJROD1-B1 ATRODD1 78.5 BJROD1-B1 BJROD1-A1 96.8 BJROD1-B1 BJROD1-A2 83.3 BJROD1-B1 BJROD1-A3 78.5 BJROD1-B1 BJROD1-B1 100 BJROD1-B1 BJROD1-B2 83.3 BJROD1-B1 BJROD1-B3 78.5 BJROD1-B1 BJROD1-B4 56.8 BJROD1-B1 BRROD1_SEQIDNO7 97.5 BJROD1-B1 CAMELINA_C1(80666) 76.5 BJROD1-B1 CAMELINA_C15(45897) 75.8 BJROD1-B1 CAMELINA_C19(65416) 75.8 BJROD1-B1 CARINATA_1B 78.5 BJROD1-B1 CARINATA_1C 79.2 BJROD1-B1 CARINATA_2B 56.8 BJROD1-B1 CARINATA_2C 56.8 BJROD1-B1 CARINATA_3B 99.3 BJROD1-B1 CARINATA_3C 98.2 BJROD1-B1 CARINATA_5B 86.8 BJROD1-B1 CARINATA_5C 86.8 BJROD1-B1 GMROD1-1 61.5 BJROD1-B1 GMROD1-2 62.8 BJROD1-B1 LUPDCT1 53.9 BJROD1-B1 LUPDCT2 53.9 BJROD1-B1 NAPUS_1A 77.8 BJROD1-B1 NAPUS_1C 79.2 BJROD1-B1 NAPUS_2A 56.8 BJROD1-B1 NAPUS_2C 56.4 BJROD1-B1 NAPUS_3A 96.8 BJROD1-B1 NAPUS_3C 98.2 BJROD1-B1 NAPUS_5A 86.8 BJROD1-B1 NAPUS_5C 87.2 BJROD1-B1 OSROD1_SEQIDNO11 43.8 BJROD1-B1 RCPDCT 60.8 BJROD1-B1 RCROD1_SEQIDNO9 60.8 BJROD1-B1 ZMROD1_GRMZM2G015040 45.8 BJROD1-B1 ZMROD1_GRMZM2G087896 44.1 BJROD1-B2 ATRODD1 78.1 BJROD1-B2 BJROD1-A1 83.7 BJROD1-B2 BJROD1-A2 87 BJROD1-B2 BJROD1-A3 76.1 BJROD1-B2 BJROD1-B1 83.3 BJROD1-B2 BJROD1-B2 100 BJROD1-B2 BJROD1-B3 77.5 BJROD1-B2 BJROD1-B4 56.1 BJROD1-B2 BRROD1_SEQIDNO7 84 BJROD1-B2 CAMELINA_C1(80666) 75.1 BJROD1-B2 CAMELINA_C15(45897) 75.2 BJROD1-B2 CAMELINA_C19(65416) 75.2 BJROD1-B2 CARINATA_1B 77.1 BJROD1-B2 CARINATA_1C 77.5 BJROD1-B2 CARINATA_2B 58.9 BJROD1-B2 CARINATA_2C 56.1 BJROD1-B2 CARINATA_3B 83.3 BJROD1-B2 CARINATA_3C 85.2 BJROD1-B2 CARINATA_5B 93.3 BJROD1-B2 CARINATA_5C 91.2 BJROD1-B2 GMROD1-1 64.1 BJROD1-B2 GMROD1-2 65 BJROD1-B2 LUPDCT1 53.8 BJROD1-B2 LUPDCT2 53.8 BJROD1-B2 NAPUS_1A 75.4 BJROD1-B2 NAPUS_1C 77.1 BJROD1-B2 NAPUS_2A 56.1 BJROD1-B2 NAPUS_2C 55.7 BJROD1-B2 NAPUS_3A 84.2 BJROD1-B2 NAPUS_3C 85.2 BJROD1-B2 NAPUS_5A 90.8 BJROD1-B2 NAPUS_5C 91.5 BJROD1-B2 OSROD1_SEQIDNO11 41.3 BJROD1-B2 RCPDCT 59.1 BJROD1-B2 RCROD1_SEQIDNO9 59.1 BJROD1-B2 ZMROD1_GRMZM2G015040 47.1 BJROD1-B2 ZMROD1_GRMZM2G087896 45.6 BJROD1-B3 ATRODD1 73.7 BJROD1-B3 BJROD1-A1 77.8 BJROD1-B3 BJROD1-A2 77.9 BJROD1-B3 BJROD1-A3 95.8 BJROD1-B3 BJROD1-B1 78.5 BJROD1-B3 BJROD1-B2 77.5 BJROD1-B3 BJROD1-B3 100 BJROD1-B3 BJROD1-B4 56.1 BJROD1-B3 BRROD1_SEQIDNO7 78.5 BJROD1-B3 CAMELINA_C1(80666) 70.4 BJROD1-B3 CAMELINA_C15(45897) 70.2 BJROD1-B3 CAMELINA_C19(65416) 70.9 BJROD1-B3 CARINATA_1B 98.6 BJROD1-B3 CARINATA_1C 94.5 BJROD1-B3 CARINATA_2B 55.4 BJROD1-B3 CARINATA_2C 56.4 BJROD1-B3 CARINATA_3B 78.8 BJROD1-B3 CARINATA_3C 78.8 BJROD1-B3 CARINATA_5B 80.2 BJROD1-B3 CARINATA_5C 78.8 BJROD1-B3 GMROD1-1 61.5 BJROD1-B3 GMROD1-2 52.7 BJROD1-B3 LUPDCT1 53.3 BJROD1-B3 LUPDCT2 53.6 BJROD1-B3 NAPUS_1A 95.2 BJROD1-B3 NAPUS_1C 95.5 BJROD1-B3 NAPUS_2A 56.1 BJROD1-B3 NAPUS_2C 55.7 BJROD1-B3 NAPUS_3A 78.3 BJROD1-B3 NAPUS_3C 78.8 BJROD1-B3 NAPUS_5A 78.9 BJROD1-B3 NAPUS_5C 79.2 BJROD1-B3 OSROD1_SEQIDNO11 43.2 BJROD1-B3 RCPDCT 57.6 BJROD1-B3 RCROD1_SEQIDNO9 57.6 BJROD1-B3 ZMROD1_GRMZM2G015040 44 BJROD1-B3 ZMROD1_GRMZM2G087896 44.7 BJROD1-B4 ATRODD1 55.5 BJROD1-B4 BJROD1-A1 57.1 BJROD1-B4 BJROD1-A2 55.4 BJROD1-B4 BJROD1-A3 55.4 BJROD1-B4 BJROD1-B1 56.8 BJROD1-B4 BJROD1-B2 59 BJROD1-B4 BJROD1-B3 56.1 BJROD1-B4 BJROD1-B4 100 BJROD1-B4 BRROD1_SEQIDNO7 57.1 BJROD1-B4 CAMELINA_C1(80666) 55.4 BJROD1-B4 CAMELINA_C15(45897) 55.2 BJROD1-B4 CAMELINA_C19(65416) 55.2 BJROD1-B4 CARINATA_1B 56.4 BJROD1-B4 CARINATA_1C 55.4 BJROD1-B4 CARINATA_2B 97.4 BJROD1-B4 CARINATA_2C 98.3 BJROD1-B4 CARINATA_3B 57.1 BJROD1-B4 CARINATA_3C 56.8 BJROD1-B4 CARINATA_5B 58.2 BJROD1-B4 CARINATA_5C 55.5 BJROD1-B4 GMROD1-1 54.9 BJROD1-B4 GMROD1-2 53.5 BJROD1-B4 LUPDCT1 48.4 BJROD1-B4 LUPDCT2 48.4 BJROD1-B4 NAPUS_1A 55.4 BJROD1-B4 NAPUS_1C 55.7 BJROD1-B4 NAPUS_2A 99.6 BJROD1-B4 NAPUS_2C 99.1 BJROD1-B4 NAPUS_3A 57.4 BJROD1-B4 NAPUS_3C 56.8 BJROD1-B4 NAPUS_5A 55.5 BJROD1-B4 NAPUS_5C 55.5 BJROD1-B4 OSROD1_SEQIDNO11 37.7 BJROD1-B4 RCPDCT 51.6 BJROD1-B4 RCROD1_SEQIDNO9 51.6 BJROD1-B4 ZMROD1_GRMZM2G015040 44.6 BJROD1-B4 ZMROD1_GRMZM2G087896 45.1 BRROD1_SEQIDNO7 ATRODD1 78.8 BRROD1_SEQIDNO7 BJROD1-A1 99.3 BRROD1_SEQIDNO7 BJROD1-A2 83 BRROD1_SEQIDNO7 BJROD1-A3 78.5 BRROD1_SEQIDNO7 BJROD1-B1 97.5 BRROD1_SEQIDNO7 BJROD1-B2 84 BRROD1_SEQIDNO7 BJROD1-B3 78.5 BRROD1_SEQIDNO7 BJROD1-B4 57.1 BRROD1_SEQIDNO7 BRROD1_SEQIDNO7 100 BRROD1_SEQIDNO7 CAMELINA_C1(80666) 76.8 BRROD1_SEQIDNO7 CAMELINA_C15(45897) 76.5 BRROD1_SEQIDNO7 CAMELINA_C19(65416) 76.5 BRROD1_SEQIDNO7 CARINATA_1B 78.5 BRROD1_SEQIDNO7 CARINATA_1C 79.5 BRROD1_SEQIDNO7 CARINATA_2B 57.1 BRROD1_SEQIDNO7 CARINATA_2C 57.1 BRROD1_SEQIDNO7 CARINATA_3B 97.5 BRROD1_SEQIDNO7 CARINATA_3C 98.6 BRROD1_SEQIDNO7 CARINATA_5B 87.5 BRROD1_SEQIDNO7 CARINATA_5C 86.8 BRROD1_SEQIDNO7 GMROD1-1 61.2 BRROD1_SEQIDNO7 GMROD1-2 63.1 BRROD1_SEQIDNO7 LUPDCT1 54.5 BRROD1_SEQIDNO7 LUPDCT2 54.5 BRROD1_SEQIDNO7 NAPUS_1A 77.8 BRROD1_SEQIDNO7 NAPUS_1C 79.2 BRROD1_SEQIDNO7 NAPUS_2A 57.1 BRROD1_SEQIDNO7 NAPUS_2C 56.8 BRROD1_SEQIDNO7 NAPUS_3A 98.9 BRROD1_SEQIDNO7 NAPUS_3C 98.6 BRROD1_SEQIDNO7 NAPUS_5A 86.8 BRROD1_SEQIDNO7 NAPUS_5C 87.2 BRROD1_SEQIDNO7 OSROD1_SEQIDNO11 41.4 BRROD1_SEQIDNO7 RCPDCT 60.8 BRROD1_SEQIDNO7 RCROD1_SEQIDNO9 60.8 BRROD1_SEQIDNO7 ZMROD1_GRMZM2G015040 46.2 BRROD1_SEQIDNO7 ZMROD1_GRMZM2G087896 44.1 CAMELINA_C1(80666) ATRODD1 86.1 CAMELINA_C1(80666) BJROD1-A1 76.8 CAMELINA_C1(80666) BJROD1-A2 71.7 CAMELINA_C1(80666) BJROD1-A3 69.1 CAMELINA_C1(80666) BJROD1-B1 76.5 CAMELINA_C1(80666) BJROD1-B2 75.1 CAMELINA_C1(80666) BJROD1-B3 70.4 CAMELINA_C1(80666) BJROD1-B4 55.4 CAMELINA_C1(80666) BRROD1_SEQIDNO7 76.8 CAMELINA_C1(80666) CAMELINA_C1(80666) 100 CAMELINA_C1(80666) CAMELINA_C15(45897) 96.6 CAMELINA_C1(80666) CAMELINA_C19(65416) 98 CAMELINA_C1(80666) CARINATA_1B 70.4 CAMELINA_C1(80666) CARINATA_1C 71.1 CAMELINA_C1(80666) CARINATA_2B 55.4 CAMELINA_C1(80666) CARINATA_2C 55.4 CAMELINA_C1(80666) CARINATA_3B 76.5 CAMELINA_C1(80666) CARINATA_3C 76.8 CAMELINA_C1(80666) CARINATA_5B 77.1 CAMELINA_C1(80666) CARINATA_5C 75.7 CAMELINA_C1(80666) GMROD1-1 60.8 CAMELINA_C1(80666) GMROD1-2 60.1 CAMELINA_C1(80666) LUPDCT1 55 CAMELINA_C1(80666) LUPDCT2 55.3 CAMELINA_C1(80666) NAPUS_1A 69.1 CAMELINA_C1(80666) NAPUS_1C 70.8 CAMELINA_C1(80666) NAPUS_2A 55.4 CAMELINA_C1(80666) NAPUS_2C 55.1 CAMELINA_C1(80666) NAPUS_3A 76.9 CAMELINA_C1(80666) NAPUS_3C 76.8 CAMELINA_C1(80666) NAPUS_5A 75.3 CAMELINA_C1(80666) NAPUS_5C 76 CAMELINA_C1(80666) OSROD1_SEQIDNO11 43.8 CAMELINA_C1(80666) RCPDCT 55.4 CAMELINA_C1(80666) RCROD1_SEQIDNO9 55.4 CAMELINA_C1(80666) ZMROD1_GRMZM2G015040 451 CAMELINA_C1(80666) ZMROD1_GRMZM2G087896 47 CAMELINA_C15(45897) ATRODD1 85.8 CAMELINA_C15(45897) BJROD1-A1 76.5 CAMELINA_C15(45897) BJROD1-A2 71.9 CAMELINA_C15(45897) BJROD1-A3 69.5 CAMELINA_C15(45897) BJROD1-B1 75.8 CAMELINA_C15(45897) BJROD1-B2 75.2 CAMELINA_C15(45897) BJROD1-B3 70.2 CAMELINA_C15(45897) BJROD1-B4 55.2 CAMELINA_C15(45897) BRROD1_SEQIDNO7 76.5 CAMELINA_C15(45897) CAMELINA_C1(80666) 96.6 CAMELINA_C15(45897) CAMELINA_C15(45897) 100 CAMELINA_C15(45897) CAMELINA_C19(65416) 97.3 CAMELINA_C15(45897) CARINATA_1B 70.2 CAMELINA_C15(45897) CARINATA_1C 70.9 CAMELINA_C15(45897) CARINATA_2B 55.2 CAMELINA_C15(45897) CARINATA_2C 55.2 CAMELINA_C15(45897) CARINATA_3B 75.8 CAMELINA_C15(45897) CARINATA_3C 76.2 CAMELINA_C15(45897) CARINATA_5B 76.8 CAMELINA_C15(45897) CARINATA_5C 76.6 CAMELINA_C15(45897) GMROD1-1 61 CAMELINA_C15(45897) GMROD1-2 60.7 CAMELINA_C15(45897) LUPDCT1 53.9 CAMELINA_C15(45897) LUPDCT2 54.2 CAMELINA_C15(45897) NAPUS_1A 69.5 CAMELINA_C15(45897) NAPUS_1C 70.5 CAMELINA_C15(45897) NAPUS_2A 55.2 CAMELINA_C15(45897) NAPUS_2C 54.9 CAMELINA_C15(45897) NAPUS_3A 76.5 CAMELINA_C15(45897) NAPUS_3C 76.2 CAMELINA_C15(45897) NAPUS_5A 75.6 CAMELINA_C15(45897) NAPUS_5C 76.9 CAMELINA_C15(45897) OSROD1_SEQIDNO11 45.4 CAMELINA_C15(45897) RCPDCT 59.8 CAMELINA_C15(45897) RCROD1_SEQIDNO9 59.8 CAMELINA_C15(45897) ZMROD1_GRMZM2G015040 45 CAMELINA_C15(45897) ZMROD1_GRMZM2G087896 46.5 CAMELINA_C19(65416) ATRODD1 86.2 CAMELINA_C19(65416) BJROD1-A1 76.5 CAMELINA_C19(65416) BJROD1-A2 72.5 CAMELINA_C19(65416) BJROD1-A3 69.5 CAMELINA_C19(65416) BJROD1-B1 75.8 CAMELINA_C19(65416) BJROD1-B2 75.2 CAMELINA_C19(65416) BJROD1-B3 70.9 CAMELINA_C19(65416) BJROD1-B4 55.2 CAMELINA_C19(65416) BRROD1_SEQIDNO7 76.5 CAMELINA_C19(65416) CAMELINA_C1(80666) 98 CAMELINA_C19(65416) CAMELINA_C15(45897) 97.3 CAMELINA_C19(65416) CAMELINA_C19(65416) 100 CAMELINA_C19(65416) CARINATA_1B 70.9 CAMELINA_C19(65416) CARINATA_1C 71.5 CAMELINA_C19(65416) CARINATA_2B 55.2 CAMELINA_C19(65416) CARINATA_2C 55.2 CAMELINA_C19(65416) CARINATA_3B 75.8 CAMELINA_C19(65416) CARINATA_3C 76.5 CAMELINA_C19(65416) CARINATA_5B 76.8 CAMELINA_C19(65416) CARINATA_5C 76.1 CAMELINA_C19(65416) GMROD1-1 60.3 CAMELINA_C19(65416) GMROD1-2 60.5 CAMELINA_C19(65416) LUPDCT1 52.6 CAMELINA_C19(65416) LUPDCT2 55.3 CAMELINA_C19(65416) NAPUS_1A 69.5 CAMELINA_C19(65416) NAPUS_1C 71.2 CAMELINA_C19(65416) NAPUS_2A 55.2 CAMELINA_C19(65416) NAPUS_2C 54.9 CAMELINA_C19(65416) NAPUS_3A 77.2 CAMELINA_C19(65416) NAPUS_3C 76.5 CAMELINA_C19(65416) NAPUS_5A 76.2 CAMELINA_C19(65416) NAPUS_5C 76.4 CAMELINA_C19(65416) OSROD1_SEQIDNO11 43.9 CAMELINA_C19(65416) RCPDCT 59.9 CAMELINA_C19(65416) RCROD1_SEQIDNO9 59.9 CAMELINA_C19(65416) ZMROD1_GRMZM2G015040 438 CAMELINA_C19(65416) ZMROD1_GRMZM2G087896 47.7 CARINATA_1B ATRODD1 73.7 CARINATA_1B BJROD1-A1 77.8 CARINATA_1B BJROD1-A2 77.9 CARINATA_1B BJROD1-A3 95.8 CARINATA_1B BJROD1-B1 78.5 CARINATA_1B BJROD1-B2 77.1 CARINATA_1B BJROD1-B3 98.6 CARINATA_1B BJROD1-B4 56.4 CARINATA_1B BRROD1_SEQIDNO7 78.5 CARINATA_1B CAMELINA_C1(80666) 70.4 CARINATA_1B CAMELINA_C15(45897) 70.2 CARINATA_1B CAMELINA_C19(65416) 70.9 CARINATA_1B CARINATA_1B 100 CARINATA_1B CARINATA_1C 93.8 CARINATA_1B CARINATA_2B 56.4 CARINATA_1B CARINATA_2C 56.7 CARINATA_1B CARINATA_3B 78.8 CARINATA_1B CARINATA_3C 78.8 CARINATA_1B CARINATA_5B 80.2 CARINATA_1B CARINATA_5C 78.8 CARINATA_1B GMROD1-1 61.1 CARINATA_1B GMROD1-2 55.3 CARINATA_1B LUPDCT1 54.1 CARINATA_1B LUPDCT2 54.5 CARINATA_1B NAPUS_1A 94.5 CARINATA_1B NAPUS_1C 94.8 CARINATA_1B NAPUS_2A 56.4 CARINATA_1B NAPUS_2C 56.1 CARINATA_1B NAPUS_3A 78.3 CARINATA_1B NAPUS_3C 78.8 CARINATA_1B NAPUS_5A 78.9 CARINATA_1B NAPUS_5C 79.2 CARINATA_1B OSROD1_SEQIDNO11 42.3 CARINATA_1B RCPDCT 57.6 CARINATA_1B RCROD1_SEQIDNO9 57.6 CARINATA_1B ZMROD1_GRMZM2G015040 44 CARINATA_1B ZMROD1_GRMZM2G087896 44.7 CARINATA_1C ATRODD1 74 CARINATA_1C BJROD1-A1 78.8 CARINATA_1C BJROD1-A2 78.3 CARINATA_1C BJROD1-A3 94.5 CARINATA_1C BJROD1-B1 79.2 CARINATA_1C BJROD1-B2 77.5 CARINATA_1C BJROD1-B3 94.5 CARINATA_1C BJROD1-B4 55.4 CARINATA_1C BRROD1_SEQIDNO7 79.5 CARINATA_1C CAMELINA_C1(80666) 71.1 CARINATA_1C CAMELINA_C15(45897) 70.9 CARINATA_1C CAMELINA_C19(65416) 71.5 CARINATA_1C CARINATA_1B 93.8 CARINATA_1C CARINATA_1C 100 CARINATA_1C CARINATA_2B 55.4 CARINATA_1C CARINATA_2C 55.7 CARINATA_1C CARINATA_3B 79.5 CARINATA_1C CARINATA_3C 79.9 CARINATA_1C CARINATA_5B 80.3 CARINATA_1C CARINATA_5C 79.2 CARINATA_1C GMROD1-1 60.3 CARINATA_1C GMROD1-2 52.9 CARINATA_1C LUPDCT1 53.3 CARINATA_1C LUPDCT2 53.6 CARINATA_1C NAPUS_1A 95.5 CARINATA_1C NAPUS_1C 99 CARINATA_1C NAPUS_2A 55.4 CARINATA_1C NAPUS_2C 55 CARINATA_1C NAPUS_3A 79.3 CARINATA_1C NAPUS_3C 79.9 CARINATA_1C NAPUS_5A 79.3 CARINATA_1C NAPUS_5C 79.5 CARINATA_1C OSROD1_SEQIDNO11 41.7 CARINATA_1C RCPDCT 57.9 CARINATA_1C RCROD1_SEQIDNO9 57.9 CARINATA_1C ZMROD1_GRMZM2G015040 42.9 CARINATA_1C ZMROD1_GRMZM2G087896 42.7 CARINATA_2B ATRODD1 55.5 CARINATA_2B BJROD1-A1 57.1 CARINATA_2B BJROD1-A2 55.4 CARINATA_2B BJROD1-A3 55 CARINATA_2B BJROD1-B1 56.8 CARINATA_2B BJROD1-B2 58.9 CARINATA_2B BJROD1-B3 55.4 CARINATA_2B BJROD1-B4 97.4 CARINATA_2B BRROD1_SEQIDNO7 57.1 CARINATA_2B CAMELINA_C1(80666) 55.4 CARINATA_2B CAMELINA_C15(45897) 55.2 CARINATA_2B CAMELINA_C19(65416) 55.2 CARINATA_2B CARINATA_1B 56.4 CARINATA_2B CARINATA_1C 55.4 CARINATA_2B CARINATA_2B 100 CARINATA_2B CARINATA_2C 99.1 CARINATA_2B CARINATA_3B 57.1 CARINATA_2B CARINATA_3C 56.8 CARINATA_2B CARINATA_5B 58.2 CARINATA_2B CARINATA_5C 55.5 CARINATA_2B GMROD1-1 55.7 CARINATA_2B GMROD1-2 54.6 CARINATA_2B LUPDCT1 49.3 CARINATA_2B LUPDCT2 49.3 CARINATA_2B NAPUS_1A 55 CARINATA_2B NAPUS_1C 55.7 CARINATA_2B NAPUS_2A 97 CARINATA_2B NAPUS_2C 96.6 CARINATA_2B NAPUS_3A 57.4 CARINATA_2B NAPUS_3C 56.8 CARINATA_2B NAPUS_5A 55.5 CARINATA_2B NAPUS_5C 55.5 CARINATA_2B OSROD1_SEQIDNO11 38.1 CARINATA_2B RCPDCT 52.3 CARINATA_2B RCROD1_SEQIDNO9 52.3 CARINATA_2B ZMROD1_GRMZM2G015040 44.9 CARINATA_2B ZMROD1_GRMZM2G087896 44.2 CARINATA_2C ATRODD1 55.5 CARINATA_2C BJROD1-A1 57.1 CARINATA_2C BJROD1-A2 55.4 CARINATA_2C BJROD1-A3 55.4 CARINATA_2C BJROD1-B1 56.8 CARINATA_2C BJROD1-B2 59 CARINATA_2C BJROD1-B3 56.4 CARINATA_2C BJROD1-B4 98.3 CARINATA_2C BRROD1_SEQIDNO7 57.1 CARINATA_2C CAMELINA_C1(80666) 55.4 CARINATA_2C CAMELINA_C15(45897) 55.2 CARINATA_2C CAMELINA_C19(65416) 55.2 CARINATA_2C CARINATA_1B 56.7 CARINATA_2C CARINATA_1C 55.7 CARINATA_2C CARINATA_2B 99.1 CARINATA_2C CARINATA_2C 100 CARINATA_2C CARINATA_3B 57.1 CARINATA_2C CARINATA_3C 56.8 CARINATA_2C CARINATA_5B 58.2 CARINATA_2C CARINATA_5C 55.5 CARINATA_2C GMROD1-1 55.3 CARINATA_2C GMROD1-2 53.9 CARINATA_2C LUPDCT1 48.7 CARINATA_2C LUPDCT2 48.7 CARINATA_2C NAPUS_1A 55.4 CARINATA_2C NAPUS_1C 56.1 CARINATA_2C NAPUS_2A 97.9 CARINATA_2C NAPUS_2C 97.4 CARINATA_2C NAPUS_3A 57.4 CARINATA_2C NAPUS_3C 56.8 CARINATA_2C NAPUS_5A 55.5 CARINATA_2C NAPUS_5C 55.5 CARINATA_2C OSROD1_SEQIDNO11 38.1 CARINATA_2C RCPDCT 51.9 CARINATA_2C RCROD1_SEQIDNO9 51.9 CARINATA_2C ZMROD1_GRMZM2G015040 45.3 CARINATA_2C ZMROD1_GRMZM2G087896 46.2 CARINATA_3B ATRODD1 78.5 CARINATA_3B BJROD1-A1 96.8 CARINATA_3B BJROD1-A2 83.3 CARINATA_3B BJROD1-A3 78.8 CARINATA_3B BJROD1-B1 99.3 CARINATA_3B BJROD1-B2 83.3 CARINATA_3B BJROD1-B3 78.8 CARINATA_3B BJROD1-B4 57.1 CARINATA_3B BRROD1_SEQIDNO7 97.5 CARINATA_3B CAMELINA_C1(80666) 76.5 CARINATA_3B CAMELINA_C15(45897) 75.8 CARINATA_3B CAMELINA_C19(65416) 75.8 CARINATA_3B CARINATA_1B 78.8 CARINATA_3B CARINATA_1C 79.5 CARINATA_3B CARINATA_2B 57.1 CARINATA_3B CARINATA_2C 57.1 CARINATA_3B CARINATA_3B 100 CARINATA_3B CARINATA_3C 98.2 CARINATA_3B CARINATA_5B 86.8 CARINATA_3B CARINATA_5C 86.8 CARINATA_3B GMROD1-1 61.9 CARINATA_3B GMROD1-2 63.1 CARINATA_3B LUPDCT1 54.2 CARINATA_3B LUPDCT2 54.2 CARINATA_3B NAPUS_1A 78.2 CARINATA_3B NAPUS_1C 79.5 CARINATA_3B NAPUS_2A 57.1 CARINATA_3B NAPUS_2C 56.8 CARINATA_3B NAPUS_3A 96.8 CARINATA_3B NAPUS_3C 98.2 CARINATA_3B NAPUS_5A 86.8 CARINATA_3B NAPUS_5C 87.2 CARINATA_3B OSROD1_SEQIDNO11 44.1 CARINATA_3B RCPDCT 61.1 CARINATA_3B RCROD1_SEQIDNO9 61.1 CARINATA_3B ZMROD1_GRMZM2G015040 46.4 CARINATA_3B ZMROD1_GRMZM2G087896 44.4 CARINATA_3C ATRODD1 78.8 CARINATA_3C BJROD1-A1 97.9 CARINATA_3C BJROD1-A2 83 CARINATA_3C BJROD1-A3 78.8 CARINATA_3C BJROD1-B1 98.2 CARINATA_3C BJROD1-B2 85.2 CARINATA_3C BJROD1-B3 78.8 CARINATA_3C BJROD1-B4 56.8 CARINATA_3C BRROD1_SEQIDNO7 98.6 CARINATA_3C CAMELINA_C1(80666) 76.8 CARINATA_3C CAMELINA_C15(45897) 76.2 CARINATA_3C CAMELINA_C19(65416) 76.5 CARINATA_3C CARINATA_1B 78.8 CARINATA_3C CARINATA_1C 79.9 CARINATA_3C CARINATA_2B 56.8 CARINATA_3C CARINATA_2C 56.8 CARINATA_3C CARINATA_3B 98.2 CARINATA_3C CARINATA_3C 100 CARINATA_3C CARINATA_5B 87.1 CARINATA_3C CARINATA_5C 86.8 CARINATA_3C GMROD1-1 61.9 CARINATA_3C GMROD1-2 63.1 CARINATA_3C LUPDCT1 54.5 CARINATA_3C LUPDCT2 54.5 CARINATA_3C NAPUS_1A 78.2 CARINATA_3C NAPUS_1C 79.5 CARINATA_3C NAPUS_2A 56.8 CARINATA_3C NAPUS_2C 56.4 CARINATA_3C NAPUS_3A 98.2 CARINATA_3C NAPUS_3C 100 CARINATA_3C NAPUS_5A 86.8 CARINATA_3C NAPUS_5C 87.2 CARINATA_3C OSROD1_SEQIDNO11 44.9 CARINATA_3C RCPDCT 60.8 CARINATA_3C RCROD1_SEQIDNO9 60.8 CARINATA_3C ZMROD1_GRMZM2G015040 45.8 CARINATA_3C ZMROD1_GRMZM2G087896 44.4 CARINATA_5B ATRODD1 80.5 CARINATA_5B BJROD1-A1 87.1 CARINATA_5B BJROD1-A2 88.8 CARINATA_5B BJROD1-A3 79.3 CARINATA_5B BJROD1-B1 86.8 CARINATA_5B BJROD1-B2 93.3 CARINATA_5B BJROD1-B3 80.2 CARINATA_5B BJROD1-B4 58.2 CARINATA_5B BRROD1_SEQIDNO7 87.5 CARINATA_5B CAMELINA_C1(80666) 77.1 CARINATA_5B CAMELINA_C15(45897) 76.8 CARINATA_5B CAMELINA_C19(65416) 76.8 CARINATA_5B CARINATA_1B 80.2 CARINATA_5B CARINATA_1C 80.3 CARINATA_5B CARINATA_2B 58.2 CARINATA_5B CARINATA_2C 58.2 CARINATA_5B CARINATA_3B 86.8 CARINATA_5B CARINATA_3C 87.1 CARINATA_5B CARINATA_5B 100 CARINATA_5B CARINATA_5C 93.7 CARINATA_5B GMROD1-1 61.7 CARINATA_5B GMROD1-2 61.2 CARINATA_5B LUPDCT1 54.1 CARINATA_5B LUPDCT2 54.1 CARINATA_5B NAPUS_1A 78.6 CARINATA_5B NAPUS_1C 79.9 CARINATA_5B NAPUS_2A 58.2 CARINATA_5B NAPUS_2C 57.8 CARINATA_5B NAPUS_3A 86.5 CARINATA_5B NAPUS_3C 87.1 CARINATA_5B NAPUS_5A 92.7 CARINATA_5B NAPUS_5C 94.1 CARINATA_5B OSROD1_SEQIDNO11 42.6 CARINATA_5B RCPDCT 59.9 CARINATA_5B RCROD1_SEQIDNO9 59.9 CARINATA_5B ZMROD1_GRMZM2G015040 46.4 CARINATA_5B ZMROD1_GRMZM2G087896 45.8 CARINATA_5C ATRODD1 79.8 CARINATA_5C BJROD1-A1 86.5 CARINATA_5C BJROD1-A2 93.3 CARINATA_5C BJROD1-A3 78.2 CARINATA_5C BJROD1-B1 86.8 CARINATA_5C BJROD1-B2 91.2 CARINATA_5C BJROD1-B3 78.8 CARINATA_5C BJROD1-B4 55.5 CARINATA_5C BRROD1_SEQIDNO7 86.8 CARINATA_5C CAMELINA_C1(80666) 75.7 CARINATA_5C CAMELINA_C15(45897) 76.6 CARINATA_5C CAMELINA_C19(65416) 76.1 CARINATA_5C CARINATA_1B 78.8 CARINATA_5C CARINATA_1C 79.2 CARINATA_5C CARINATA_2B 55.5 CARINATA_5C CARINATA_2C 55.5 CARINATA_5C CARINATA_3B 86.8 CARINATA_5C CARINATA_3C 86.8 CARINATA_5C CARINATA_5B 93.7 CARINATA_5C CARINATA_5C 100 CARINATA_5C GMROD1-1 60.3 CARINATA_5C GMROD1-2 61.1 CARINATA_5C LUPDCT1 51.7 CARINATA_5C LUPDCT2 51.7 CARINATA_5C NAPUS_1A 77.5 CARINATA_5C NAPUS_1C 78.8 CARINATA_5C NAPUS_2A 55.5 CARINATA_5C NAPUS_2C 55.1 CARINATA_5C NAPUS_3A 85.5 CARINATA_5C NAPUS_3C 86.8 CARINATA_5C NAPUS_5A 97.5 CARINATA_5C NAPUS_5C 99.6 CARINATA_5C OSROD1_SEQIDNO11 42.5 CARINATA_5C RCPDCT 59.9 CARINATA_5C RCROD1_SEQIDNO9 59.9 CARINATA_5C ZMROD1_GRMZM2G015040 46.4 CARINATA_5C ZMROD1_GRMZM2G087896 44.8 GMROD1-1 ATRODD1 60.7 GMROD1-1 BJROD1-A1 62.4 GMROD1-1 BJROD1-A2 62.1 GMROD1-1 BJROD1-A3 60.2 GMROD1-1 BJROD1-B1 61.5 GMROD1-1 BJROD1-B2 64.1 GMROD1-1 BJROD1-B3 61.5 GMROD1-1 BJROD1-B4 54.9 GMROD1-1 BRROD1_SEQIDNO7 61.2 GMROD1-1 CAMELINA_C1(80666) 60.8 GMROD1-1 CAMELINA_C15(45897) 61 GMROD1-1 CAMELINA_C19(65416) 60.3 GMROD1-1 CARINATA_1B 61.1 GMROD1-1 CARINATA_1C 60.3 GMROD1-1 CARINATA_2B 55.7 GMROD1-1 CARINATA_2C 55.3 GMROD1-1 CARINATA_3B 61.9 GMROD1-1 CARINATA_3C 61.9 GMROD1-1 CARINATA_5B 61.7 GMROD1-1 CARINATA_5C 60.3 GMROD1-1 GMROD1-1 100 GMROD1-1 GMROD1-2 86.3 GMROD1-1 LUPDCT1 60.1 GMROD1-1 LUPDCT2 60.1 GMROD1-1 NAPUS_1A 60.5 GMROD1-1 NAPUS_1C 60.3 GMROD1-1 NAPUS_2A 54.9 GMROD1-1 NAPUS_2C 54.6 GMROD1-1 NAPUS_3A 61.2 GMROD1-1 NAPUS_3C 61.9 GMROD1-1 NAPUS_5A 62.3 GMROD1-1 NAPUS_5C 60.3 GMROD1-1 OSROD1_SEQIDNO11 47.1 GMROD1-1 RCPDCT 68.2 GMROD1-1 RCROD1_SEQIDNO9 68.2 GMROD1-1 ZMROD1_GRMZM2G015040 51.4 GMROD1-1 ZMROD1_GRMZM2G087896 53.1 GMROD1-2 ATRODD1 58.1 GMROD1-2 BJROD1-A1 62.8 GMROD1-2 BJROD1-A2 57.5 GMROD1-2 BJROD1-A3 54.4 GMROD1-2 BJROD1-B1 62.8 GMROD1-2 BJROD1-B2 65 GMROD1-2 BJROD1-B3 52.7 GMROD1-2 BJROD1-B4 53.5 GMROD1-2 BRROD1_SEQIDNO7 63.1 GMROD1-2 CAMELINA_C1(80666) 60.1 GMROD1-2 CAMELINA_C15(45897) 60.7 GMROD1-2 CAMELINA_C19(65416) 60.5 GMROD1-2 CARINATA_1B 55.3 GMROD1-2 CARINATA_1C 52.9 GMROD1-2 CARINATA_2B 54.6 GMROD1-2 CARINATA_2C 53.9 GMROD1-2 CARINATA_3B 63.1 GMROD1-2 CARINATA_3C 63.1 GMROD1-2 CARINATA_5B 61.2 GMROD1-2 CARINATA_5C 61.1 GMROD1-2 GMROD1-1 86.3 GMROD1-2 GMROD1-2 100 GMROD1-2 LUPDCT1 56.1 GMROD1-2 LUPDCT2 56.1 GMROD1-2 NAPUS_1A 54.4 GMROD1-2 NAPUS_1C 52.9 GMROD1-2 NAPUS_2A 53.5 GMROD1-2 NAPUS_2C 53.2 GMROD1-2 NAPUS_3A 62.7 GMROD1-2 NAPUS_3C 63.1 GMROD1-2 NAPUS_5A 61 GMROD1-2 NAPUS_5C 61.1 GMROD1-2 OSROD1_SEQIDNO11 46.5 GMROD1-2 RCPDCT 59.3 GMROD1-2 RCROD1_SEQIDNO9 59.3 GMROD1-2 ZMROD1_GRMZM2G015040 50.9 GMROD1-2 ZMROD1_GRMZM2G087896 49 LUPDCT1 ATRODD1 54.6 LUPDCT1 BJROD1-A1 54.5 LUPDCT1 BJROD1-A2 51.5 LUPDCT1 BJROD1-A3 52.5 LUPDCT1 BJROD1-B1 53.9 LUPDCT1 BJROD1-B2 53.8 LUPDCT1 BJROD1-B3 53.3 LUPDCT1 BJROD1-B4 48.4 LUPDCT1 BRROD1_SEQIDNO7 54.5 LUPDCT1 CAMELINA_C1(80666) 55 LUPDCT1 CAMELINA_C15(45897) 53.9 LUPDCT1 CAMELINA_C19(65416) 52.6 LUPDCT1 CARINATA_1B 54.1 LUPDCT1 CARINATA_1C 53.3 LUPDCT1 CARINATA_2B 49.3 LUPDCT1 CARINATA_2C 48.7 LUPDCT1 CARINATA_3B 54.2 LUPDCT1 CARINATA_3C 54.5 LUPDCT1 CARINATA_5B 54.1 LUPDCT1 CARINATA_5C 51.7 LUPDCT1 GMROD1-1 60.1 LUPDCT1 GMROD1-2 56.1 LUPDCT1 LUPDCT1 100 LUPDCT1 LUPDCT2 98.6 LUPDCT1 NAPUS_1A 52.9 LUPDCT1 NAPUS_1C 53.3 LUPDCT1 NAPUS_2A 48.4 LUPDCT1 NAPUS_2C 48 LUPDCT1 NAPUS_3A 54.9 LUPDCT1 NAPUS_3C 54.5 LUPDCT1 NAPUS_5A 52 LUPDCT1 NAPUS_5C 52 LUPDCT1 OSROD1_SEQIDNO11 45.9 LUPDCT1 RCPDCT 59.2 LUPDCT1 RCROD1_SEQIDNO9 59.2 LUPDCT1 ZMROD1_GRMZM2G015040 48.1 LUPDCT1 ZMROD1_GRMZM2G087896 49 LUPDCT2 ATRODD1 54.2 LUPDCT2 BJROD1-A1 54.5 LUPDCT2 BJROD1-A2 51.5 LUPDCT2 BJROD1-A3 53.4 LUPDCT2 BJROD1-B1 53.9 LUPDCT2 BJROD1-B2 53.8 LUPDCT2 BJROD1-B3 53.6 LUPDCT2 BJROD1-B4 48.4 LUPDCT2 BRROD1_SEQIDNO7 54.5 LUPDCT2 CAMELINA_C1(80666) 55.3 LUPDCT2 CAMELINA_C15(45897) 54.2 LUPDCT2 CAMELINA_C19(65416) 55.3 LUPDCT2 CARINATA_1B 54.5 LUPDCT2 CARINATA_1C 53.6 LUPDCT2 CARINATA_2B 49.3 LUPDCT2 CARINATA_2C 48.7 LUPDCT2 CARINATA_3B 54.2 LUPDCT2 CARINATA_3C 54.5 LUPDCT2 CARINATA_5B 54.1 LUPDCT2 CARINATA_5C 51.7 LUPDCT2 GMROD1-1 60.1 LUPDCT2 GMROD1-2 56.1 LUPDCT2 LUPDCT1 98.6 LUPDCT2 LUPDCT2 100 LUPDCT2 NAPUS_1A 53.8 LUPDCT2 NAPUS_1C 53.6 LUPDCT2 NAPUS_2A 48.4 LUPDCT2 NAPUS_2C 48 LUPDCT2 NAPUS_3A 54.9 LUPDCT2 NAPUS_3C 54.5 LUPDCT2 NAPUS_5A 52 LUPDCT2 NAPUS_5C 52 LUPDCT2 OSROD1_SEQIDNO11 46.3 LUPDCT2 RCPDCT 59.2 LUPDCT2 RCROD1_SEQIDNO9 59.2 LUPDCT2 ZMROD1_GRMZM2G015040 47.8 LUPDCT2 ZMROD1_GRMZM2G087896 48.6 NAPUS_1A ATRODD1 72.7 NAPUS_1A BJROD1-A1 77.1 NAPUS_1A BJROD1-A2 76.6 NAPUS_1A BJROD1-A3 98.6 NAPUS_1A BJROD1-B1 77.8 NAPUS_1A BJROD1-B2 75.4 NAPUS_1A BJROD1-B3 95.2 NAPUS_1A BJROD1-B4 55.4 NAPUS_1A BRROD1_SEQIDNO7 77.8 NAPUS_1A CAMELINA_C1(80666) 69.1 NAPUS_1A CAMELINA_C15(45897) 69.5 NAPUS_1A CAMELINA_C19(65416) 69.5 NAPUS_1A CARINATA_1B 94.5 NAPUS_1A CARINATA_1C 95.5 NAPUS_1A CARINATA_2B 55 NAPUS_1A CARINATA_2C 55.4 NAPUS_1A CARINATA_3B 78.2 NAPUS_1A CARINATA_3C 78.2 NAPUS_1A CARINATA_5B 78.6 NAPUS_1A CARINATA_5C 77.5 NAPUS_1A GMROD1-1 60.5 NAPUS_1A GMROD1-2 54.4 NAPUS_1A LUPDCT1 52.9 NAPUS_1A LUPDCT2 53.8 NAPUS_1A NAPUS_1A 100 NAPUS_1A NAPUS_1C 96.5 NAPUS_1A NAPUS_2A 55.4 NAPUS_1A NAPUS_2C 55 NAPUS_1A NAPUS_3A 77.6 NAPUS_1A NAPUS_3C 78.2 NAPUS_1A NAPUS_5A 77.6 NAPUS_1A NAPUS_5C 77.8 NAPUS_1A OSROD1_SEQIDNO11 42.4 NAPUS_1A RCPDCT 57.3 NAPUS_1A RCROD1_SEQIDNO9 57.3 NAPUS_1A ZMROD1_GRMZM2G015040 44 NAPUS_1A ZMROD1_GRMZM2G087896 43 NAPUS_1C ATRODD1 73.7 NAPUS_1C BJROD1-A1 78.5 NAPUS_1C BJROD1-A2 77.9 NAPUS_1C BJROD1-A3 95.5 NAPUS_1C BJROD1-B1 79.2 NAPUS_1C BJROD1-B2 77.1 NAPUS_1C BJROD1-B3 95.5 NAPUS_1C BJROD1-B4 55.7 NAPUS_1C BRROD1_SEQIDNO7 79.2 NAPUS_1C CAMELINA_C1(80666) 70.8 NAPUS_1C CAMELINA_C15(45897) 70.5 NAPUS_1C CAMELINA_C19(65416) 71.2 NAPUS_1C CARINATA_1B 94.8 NAPUS_1C CARINATA_1C 99 NAPUS_1C CARINATA_2B 55.7 NAPUS_1C CARINATA_2C 56.1 NAPUS_1C CARINATA_3B 79.5 NAPUS_1C CARINATA_3C 79.5 NAPUS_1C CARINATA_5B 79.9 NAPUS_1C CARINATA_5C 78.8 NAPUS_1C GMROD1-1 60.3 NAPUS_1C GMROD1-2 52.9 NAPUS_1C LUPDCT1 53.3 NAPUS_1C LUPDCT2 53.6 NAPUS_1C NAPUS_1A 96.5 NAPUS_1C NAPUS_1C 100 NAPUS_1C NAPUS_2A 55.7 NAPUS_1C NAPUS_2C 55.4 NAPUS_1C NAPUS_3A 79 NAPUS_1C NAPUS_3C 79.5 NAPUS_1C NAPUS_5A 78.9 NAPUS_1C NAPUS_5C 79.2 NAPUS_1C OSROD1_SEQIDNO11 42 NAPUS_1C RCPDCT 57.9 NAPUS_1C RCROD1_SEQIDNO9 57.9 NAPUS_1C ZMROD1_GRMZM2G015040 43.3 NAPUS_1C ZMROD1_GRMZM2G087896 43 NAPUS_2A ATRODD1 55.5 NAPUS_2A BJROD1-A1 57.1 NAPUS_2A BJROD1-A2 55.4 NAPUS_2A BJROD1-A3 55.4 NAPUS_2A BJROD1-B1 56.8 NAPUS_2A BJROD1-B2 59 NAPUS_2A BJROD1-B3 56.1 NAPUS_2A BJROD1-B4 99.6 NAPUS_2A BRROD1_SEQIDNO7 57.1 NAPUS_2A CAMELINA_C1(80666) 55.4 NAPUS_2A CAMELINA_C15(45897) 55.2 NAPUS_2A CAMELINA_C19(65416) 55.2 NAPUS_2A CARINATA_1B 56.4 NAPUS_2A CARINATA_1C 55.4 NAPUS_2A CARINATA_2B 97 NAPUS_2A CARINATA_2C 97.9 NAPUS_2A CARINATA_3B 57.1 NAPUS_2A CARINATA_3C 56.8 NAPUS_2A CARINATA_5B 58.2 NAPUS_2A CARINATA_5C 55.5 NAPUS_2A GMROD1-1 54.9 NAPUS_2A GMROD1-2 53.5 NAPUS_2A LUPDCT1 48.4 NAPUS_2A LUPDCT2 48.4 NAPUS_2A NAPUS_1A 55.4 NAPUS_2A NAPUS_1C 55.7 NAPUS_2A NAPUS_2A 100 NAPUS_2A NAPUS_2C 99.6 NAPUS_2A NAPUS_3A 57.4 NAPUS_2A NAPUS_3C 56.8 NAPUS_2A NAPUS_5A 55.5 NAPUS_2A NAPUS_5C 55.5 NAPUS_2A OSROD1_SEQIDNO11 38.1 NAPUS_2A RCPDCT 51.6 NAPUS_2A RCROD1_SEQIDNO9 51.6 NAPUS_2A ZMROD1_GRMZM2G015040 44.9 NAPUS_2A ZMROD1_GRMZM2G087896 45.5 NAPUS_2C ATRODD1 55.1 NAPUS_2C BJROD1-A1 56.8 NAPUS_2C BJROD1-A2 55.1 NAPUS_2C BJROD1-A3 55 NAPUS_2C BJROD1-B1 56.4 NAPUS_2C BJROD1-B2 58.6 NAPUS_2C BJROD1-B3 55.7 NAPUS_2C BJROD1-B4 99.1 NAPUS_2C BRROD1_SEQIDNO7 56.8 NAPUS_2C CAMELINA_C1(80666) 55.1 NAPUS_2C CAMELINA_C15(45897) 54.9 NAPUS_2C CAMELINA_C19(65416) 54.9 NAPUS_2C CARINATA_1B 56.1 NAPUS_2C CARINATA_1C 55 NAPUS_2C CARINATA_2B 96.6 NAPUS_2C CARINATA_2C 97.4 NAPUS_2C CARINATA_3B 56.8 NAPUS_2C CARINATA_3C 56.4 NAPUS_2C CARINATA_5B 57.8 NAPUS_2C CARINATA_5C 55.1 NAPUS_2C GMROD1-1 54.6 NAPUS_2C GMROD1-2 53.2 NAPUS_2C LUPDCT1 48 NAPUS_2C LUPDCT2 48 NAPUS_2C NAPUS_1A 55 NAPUS_2C NAPUS_1C 55.4 NAPUS_2C NAPUS_2A 99.6 NAPUS_2C NAPUS_2C 100 NAPUS_2C NAPUS_3A 57.1 NAPUS_2C NAPUS_3C 56.4 NAPUS_2C NAPUS_5A 55.1 NAPUS_2C NAPUS_5C 55.1 NAPUS_2C OSROD1_SEQIDNO11 38.1 NAPUS_2C RCPDCT 51.2 NAPUS_2C RCROD1_SEQIDNO9 51.2 NAPUS_2C ZMROD1_GRMZM2G015040 44.6 NAPUS_2C ZMROD1_GRMZM2G087896 45.1 NAPUS_3A ATRODD1 79.2 NAPUS_3A BJROD1-A1 98.2 NAPUS_3A BJROD1-A2 81.7 NAPUS_3A BJROD1-A3 78.3 NAPUS_3A BJROD1-B1 96.8 NAPUS_3A BJROD1-B2 84.2 NAPUS_3A BJROD1-B3 78.3 NAPUS_3A BJROD1-B4 57.4 NAPUS_3A BRROD1_SEQIDNO7 98.9 NAPUS_3A CAMELINA_C1(80666) 76.9 NAPUS_3A CAMELINA_C15(45897) 76.5 NAPUS_3A CAMELINA_C19(65416) 77.2 NAPUS_3A CARINATA_1B 78.3 NAPUS_3A CARINATA_1C 79.3 NAPUS_3A CARINATA_2B 57.4 NAPUS_3A CARINATA_2C 57.4 NAPUS_3A CARINATA_3B 96.8 NAPUS_3A CARINATA_3C 98.2 NAPUS_3A CARINATA_5B 86.5 NAPUS_3A CARINATA_5C 85.5 NAPUS_3A GMROD1-1 61.2 NAPUS_3A GMROD1-2 62.7 NAPUS_3A LUPDCT1 54.9 NAPUS_3A LUPDCT2 54.9 NAPUS_3A NAPUS_1A 77.6 NAPUS_3A NAPUS_1C 79 NAPUS_3A NAPUS_2A 57.4 NAPUS_3A NAPUS_2C 57.1 NAPUS_3A NAPUS_3A 100 NAPUS_3A NAPUS_3C 98.2 NAPUS_3A NAPUS_5A 85.5 NAPUS_3A NAPUS_5C 85.9 NAPUS_3A OSROD1_SEQIDNO11 44.6 NAPUS_3A RCPDCT 61 NAPUS_3A RCROD1_SEQIDNO9 61 NAPUS_3A ZMROD1_GRMZM2G015040 45.9 NAPUS_3A ZMROD1_GRMZM2G087896 43.8 NAPUS_3C ATRODD1 78.8 NAPUS_3C BJROD1-A1 97.9 NAPUS_3C BJROD1-A2 83 NAPUS_3C BJROD1-A3 78.8 NAPUS_3C BJROD1-B1 98.2 NAPUS_3C BJROD1-B2 85.2 NAPUS_3C BJROD1-B3 78.8 NAPUS_3C BJROD1-B4 56.8 NAPUS_3C BRROD1_SEQIDNO7 98.6 NAPUS_3C CAMELINA_C1(80666) 76.8 NAPUS_3C CAMELINA_C15(45897) 76.2 NAPUS_3C CAMELINA_C19(65416) 76.5 NAPUS_3C CARINATA_1B 78.8 NAPUS_3C CARINATA_1C 79.9 NAPUS_3C CARINATA_2B 56.8 NAPUS_3C CARINATA_2C 56.8 NAPUS_3C CARINATA_3B 98.2 NAPUS_3C CARINATA_3C 100 NAPUS_3C CARINATA_5B 87.1 NAPUS_3C CARINATA_5C 86.8 NAPUS_3C GMROD1-1 61.9 NAPUS_3C GMROD1-2 63.1 NAPUS_3C LUPDCT1 54.5 NAPUS_3C LUPDCT2 54.5 NAPUS_3C NAPUS_1A 78.2 NAPUS_3C NAPUS_1C 79.5 NAPUS_3C NAPUS_2A 56.8 NAPUS_3C NAPUS_2C 56.4 NAPUS_3C NAPUS_3A 98.2 NAPUS_3C NAPUS_3C 100 NAPUS_3C NAPUS_5A 86.8 NAPUS_3C NAPUS_5C 87.2 NAPUS_3C OSROD1_SEQIDNO11 44.9 NAPUS_3C RCPDCT 60.8 NAPUS_3C RCROD1_SEQIDNO9 60.8 NAPUS_3C ZMROD1_GRMZM2G015040 45.8 NAPUS_3C ZMROD1_GRMZM2G087896 44.4 NAPUS_5A ATRODD1 79.7 NAPUS_5A BJROD1-A1 86.5 NAPUS_5A BJROD1-A2 95.4 NAPUS_5A BJROD1-A3 78.2 NAPUS_5A BJROD1-B1 86.8 NAPUS_5A BJROD1-B2 90.8 NAPUS_5A BJROD1-B3 78.9 NAPUS_5A BJROD1-B4 55.5 NAPUS_5A BRROD1_SEQIDNO7 86.8 NAPUS_5A CAMELINA_C1(80666) 75.3 NAPUS_5A CAMELINA_C15(45897) 75.6 NAPUS_5A CAMELINA_C19(65416) 76.2 NAPUS_5A CARINATA_1B 78.9 NAPUS_5A CARINATA_1C 79.3 NAPUS_5A CARINATA_2B 55.5 NAPUS_5A CARINATA_2C 55.5 NAPUS_5A CARINATA_3B 86.8 NAPUS_5A CARINATA_3C 86.8 NAPUS_5A CARINATA_5B 92.7 NAPUS_5A CARINATA_5C 97.5 NAPUS_5A GMROD1-1 62.3 NAPUS_5A GMROD1-2 61 NAPUS_5A LUPDCT1 52 NAPUS_5A LUPDCT2 52 NAPUS_5A NAPUS_1A 77.6 NAPUS_5A NAPUS_1C 78.9 NAPUS_5A NAPUS_2A 55.5 NAPUS_5A NAPUS_2C 55.1 NAPUS_5A NAPUS_3A 85.5 NAPUS_5A NAPUS_3C 86.8 NAPUS_5A NAPUS_5A 100 NAPUS_5A NAPUS_5C 97.9 NAPUS_5A OSROD1_SEQIDNO11 42.2 NAPUS_5A RCPDCT 60.2 NAPUS_5A RCROD1_SEQIDNO9 60.2 NAPUS_5A ZMROD1_GRMZM2G015040 45.2 NAPUS_5A ZMROD1_GRMZM2G087896 45.6 NAPUS_5C ATRODD1 80.1 NAPUS_5C BJROD1-A1 86.8 NAPUS_5C BJROD1-A2 93.6 NAPUS_5C BJROD1-A3 78.5 NAPUS_5C BJROD1-B1 87.2 NAPUS_5C BJROD1-B2 91.5 NAPUS_5C BJROD1-B3 79.2 NAPUS_5C BJROD1-B4 55.5 NAPUS_5C BRROD1_SEQIDNO7 87.2 NAPUS_5C CAMELINA_C1(80666) 76 NAPUS_5C CAMELINA_C15(45897) 76.9 NAPUS_5C CAMELINA_C19(65416) 76.4 NAPUS_5C CARINATA_1B 79.2 NAPUS_5C CARINATA_1C 79.5 NAPUS_5C CARINATA_2B 55.5 NAPUS_5C CARINATA_2C 55.5 NAPUS_5C CARINATA_3B 87.2 NAPUS_5C CARINATA_3C 87.2 NAPUS_5C CARINATA_5B 94.1 NAPUS_5C CARINATA_5C 99.6 NAPUS_5C GMROD1-1 60.3 NAPUS_5C GMROD1-2 61.1 NAPUS_5C LUPDCT1 52 NAPUS_5C LUPDCT2 52 NAPUS_5C NAPUS_1A 77.8 NAPUS_5C NAPUS_1C 79.2 NAPUS_5C NAPUS_2A 55.5 NAPUS_5C NAPUS_2C 55.1 NAPUS_5C NAPUS_3A 85.9 NAPUS_5C NAPUS_3C 87.2 NAPUS_5C NAPUS_5A 97.9 NAPUS_5C NAPUS_5C 100 NAPUS_5C OSROD1_SEQIDNO11 42.5 NAPUS_5C RCPDCT 59.9 NAPUS_5C RCROD1_SEQIDNO9 59.9 NAPUS_5C ZMROD1_GRMZM2G015040 46.4 NAPUS_5C ZMROD1_GRMZM2G087896 44.8 OSROD1_SEQIDNO11 ATRODD1 45.5 OSROD1_SEQIDNO11 BJROD1-A1 45.3 OSROD1_SEQIDNO11 BJROD1-A2 42.2 OSROD1_SEQIDNO11 BJROD1-A3 41.8 OSROD1_SEQIDNO11 BJROD1-B1 43.8 OSROD1_SEQIDNO11 BJROD1-B2 41.3 OSROD1_SEQIDNO11 BJROD1-B3 43.2 OSROD1_SEQIDNO11 BJROD1-B4 37.7 OSROD1_SEQIDNO11 BRROD1_SEQIDNO7 41.4 OSROD1_SEQIDNO11 CAMELINA_C1(80666) 43.8 OSROD1_SEQIDNO11 CAMELINA_C15(45897) 45.4 OSROD1_SEQIDNO11 CAMELINA_C19(65416) 43.9 OSROD1_SEQIDNO11 CARINATA_1B 42.3 OSROD1_SEQIDNO11 CARINATA_1C 41.7 OSROD1_SEQIDNO11 CARINATA_2B 38.1 OSROD1_SEQIDNO11 CARINATA_2C 38.1 OSROD1_SEQIDNO11 CARINATA_3B 44.1 OSROD1_SEQIDNO11 CARINATA_3C 44.9 OSROD1_SEQIDNO11 CARINATA_5B 42.6 OSROD1_SEQIDNO11 CARINATA_5C 42.5 OSROD1_SEQIDNO11 GMROD1-1 47.1 OSROD1_SEQIDNO11 GMROD1-2 46.5 OSROD1_SEQIDNO11 LUPDCT1 45.9 OSROD1_SEQIDNO11 LUPDCT2 46.3 OSROD1_SEQIDNO11 NAPUS_1A 42.4 OSROD1_SEQIDNO11 NAPUS_1C 42 OSROD1_SEQIDNO11 NAPUS_2A 38.1 OSROD1_SEQIDNO11 NAPUS_2C 38.1 OSROD1_SEQIDNO11 NAPUS_3A 44.6 OSROD1_SEQIDNO11 NAPUS_3C 44.9 OSROD1_SEQIDNO11 NAPUS_5A 42.2 OSROD1_SEQIDNO11 NAPUS_5C 42.5 OSROD1_SEQIDNO11 OSROD1_SEQIDNO11 100 OSROD1_SEQIDNO11 RCPDCT 48.9 OSROD1_SEQIDNO11 RCROD1_SEQIDNO9 48.9 OSROD1_SEQIDNO11 ZMROD1_GRMZM2G015040 69.1 OSROD1_SEQIDNO11 ZMROD1_GRMZM2G087896 68.9 RCPDCT ATRODD1 58.7 RCPDCT BJROD1-A1 58.6 RCPDCT BJROD1-A2 59.7 RCPDCT BJROD1-A3 57 RCPDCT BJROD1-B1 60.8 RCPDCT BJROD1-B2 59.1 RCPDCT BJROD1-B3 57.6 RCPDCT BJROD1-B4 51.6 RCPDCT BRROD1_SEQIDNO7 60.8 RCPDCT CAMELINA_C1(80666) 55.4 RCPDCT CAMELINA_C15(45897) 59.8 RCPDCT CAMELINA_C19(65416) 59.9 RCPDCT CARINATA_1B 57.6 RCPDCT CARINATA_1C 57.9 RCPDCT CARINATA_2B 52.3 RCPDCT CARINATA_2C 51.9 RCPDCT CARINATA_3B 61.1 RCPDCT CARINATA_3C 60.8 RCPDCT CARINATA_5B 59.9 RCPDCT CARINATA_5C 59.9 RCPDCT GMROD1-1 68.2 RCPDCT GMROD1-2 59.3 RCPDCT LUPDCT1 59.2 RCPDCT LUPDCT2 59.2 RCPDCT NAPUS_1A 57.3 RCPDCT NAPUS_1C 57.9 RCPDCT NAPUS_2A 51.6 RCPDCT NAPUS_2C 51.2 RCPDCT NAPUS_3A 61 RCPDCT NAPUS_3C 60.8 RCPDCT NAPUS_5A 60.2 RCPDCT NAPUS_5C 59.9 RCPDCT OSROD1_SEQIDNO11 48.9 RCPDCT RCPDCT 100 RCPDCT RCROD1_SEQIDNO9 100 RCPDCT ZMROD1_GRMZM2G015040 51.3 RCPDCT ZMROD1_GRMZM2G087896 48.2 RCROD1_SEQIDNO9 ATRODD1 58.7 RCROD1_SEQIDNO9 BJROD1-A1 58.6 RCROD1_SEQIDNO9 BJROD1-A2 59.7 RCROD1_SEQIDNO9 BJROD1-A3 57 RCROD1_SEQIDNO9 BJROD1-B1 60.8 RCROD1_SEQIDNO9 BJROD1-B2 59.1 RCROD1_SEQIDNO9 BJROD1-B3 57.6 RCROD1_SEQIDNO9 BJROD1-B4 51.6 RCROD1_SEQIDNO9 BRROD1_SEQIDNO7 60.8 RCROD1_SEQIDNO9 CAMELINA_C1(80666) 55.4 RCROD1_SEQIDNO9 CAMELINA_C15(45897) 59.8 RCROD1_SEQIDNO9 CAMELINA_C19(65416) 59.9 RCROD1_SEQIDNO9 CARINATA_1B 57.6 RCROD1_SEQIDNO9 CARINATA_1C 57.9 RCROD1_SEQIDNO9 CARINATA_2B 52.3 RCROD1_SEQIDNO9 CARINATA_2C 51.9 RCROD1_SEQIDNO9 CARINATA_3B 61.1 RCROD1_SEQIDNO9 CARINATA_3C 60.8 RCROD1_SEQIDNO9 CARINATA_5B 59.9 RCROD1_SEQIDNO9 CARINATA_5C 59.9 RCROD1_SEQIDNO9 GMROD1-1 68.2 RCROD1_SEQIDNO9 GMROD1-2 59.3 RCROD1_SEQIDNO9 LUPDCT1 59.2 RCROD1_SEQIDNO9 LUPDCT2 59.2 RCROD1_SEQIDNO9 NAPUS_1A 57.3 RCROD1_SEQIDNO9 NAPUS_1C 57.9 RCROD1_SEQIDNO9 NAPUS_2A 51.6 RCROD1_SEQIDNO9 NAPUS_2C 51.2 RCROD1_SEQIDNO9 NAPUS_3A 61 RCROD1_SEQIDNO9 NAPUS_3C 60.8 RCROD1_SEQIDNO9 NAPUS_5A 60.2 RCROD1_SEQIDNO9 NAPUS_5C 59.9 RCROD1_SEQIDNO9 OSROD1_SEQIDNO11 48.9 RCROD1_SEQIDNO9 RCPDCT 100 RCROD1_SEQIDNO9 RCROD1_SEQIDNO9 100 RCROD1_SEQIDNO9 ZMROD1_GRMZM2G015040 51.3 RCROD1_SEQIDNO9 ZMROD1_GRMZM2G087896 48.2 ZMROD1_GRMZM2G015040 ATRODD1 44.4 ZMROD1_GRMZM2G015040 BJROD1-A1 45.3 ZMROD1_GRMZM2G015040 BJROD1-A2 45.1 ZMROD1_GRMZM2G015040 BJROD1-A3 43.7 ZMROD1_GRMZM2G015040 BJROD1-B1 45.8 ZMROD1_GRMZM2G015040 BJROD1-B2 47.1 ZMROD1_GRMZM2G015040 BJROD1-B3 44 ZMROD1_GRMZM2G015040 BJROD1-B4 44.6 ZMROD1_GRMZM2G015040 BRROD1_SEQIDNO7 46.2 ZMROD1_GRMZM2G015040 CAMELINA_C1(80666) 45.1 ZMROD1_GRMZM2G015040 CAMELINA_C15(45897) 45 ZMROD1_GRMZM2G015040 CAMELINA_C19(65416) 43.8 ZMROD1_GRMZM2G015040 CARINATA_1B 44 ZMROD1_GRMZM2G015040 CARINATA_1C 42.9 ZMROD1_GRMZM2G015040 CARINATA_2B 44.9 ZMROD1_GRMZM2G015040 CARINATA_2C 45.3 ZMROD1_GRMZM2G015040 CARINATA_3B 46.4 ZMROD1_GRMZM2G015040 CARINATA_3C 45.8 ZMROD1_GRMZM2G015040 CARINATA_5B 46.4 ZMROD1_GRMZM2G015040 CARINATA_5C 46.4 ZMROD1_GRMZM2G015040 GMROD1-1 51.4 ZMROD1_GRMZM2G015040 GMROD1-2 50.9 ZMROD1_GRMZM2G015040 LUPDCT1 48.1 ZMROD1_GRMZM2G015040 LUPDCT2 47.8 ZMROD1_GRMZM2G015040 NAPUS_1A 44 ZMROD1_GRMZM2G015040 NAPUS_1C 43.3 ZMROD1_GRMZM2G015040 NAPUS_2A 44.9 ZMROD1_GRMZM2G015040 NAPUS_2C 44.6 ZMROD1_GRMZM2G015040 NAPUS_3A 45.9 ZMROD1_GRMZM2G015040 NAPUS_3C 45.8 ZMROD1_GRMZM2G015040 NAPUS_5A 45.2 ZMROD1_GRMZM2G015040 NAPUS_5C 46.4 ZMROD1_GRMZM2G015040 OSROD1_SEQIDNO11 69.1 ZMROD1_GRMZM2G015040 RCPDCT 51.3 ZMROD1_GRMZM2G015040 RCROD1_SEQIDNO9 51.3 ZMROD1_GRMZM2G015040 ZMROD1_GRMZM2G015040 100 ZMROD1_GRMZM2G015040 ZMROD1_GRMZM2G087896 83.9 ZMROD1_GRMZM2G087896 ATRODD1 42.9 ZMROD1_GRMZM2G087896 BJROD1-A1 44.1 ZMROD1_GRMZM2G087896 BJROD1-A2 45.6 ZMROD1_GRMZM2G087896 BJROD1-A3 42.7 ZMROD1_GRMZM2G087896 BJROD1-B1 44.1 ZMROD1_GRMZM2G087896 BJROD1-B2 45.6 ZMROD1_GRMZM2G087896 BJROD1-B3 44.7 ZMROD1_GRMZM2G087896 BJROD1-B4 43.6 ZMROD1_GRMZM2G087896 BRROD1_SEQIDNO7 44.1 ZMROD1_GRMZM2G087896 CAMELINA_C1(80666) 47 ZMROD1_GRMZM2G087896 CAMELINA_C15(45897) 46.5 ZMROD1_GRMZM2G087896 CAMELINA_C19(65416) 47.7 ZMROD1_GRMZM2G087896 CARINATA_1B 44.7 ZMROD1_GRMZM2G087896 CARINATA_1C 42.7 ZMROD1_GRMZM2G087896 CARINATA_2B 44.2 ZMROD1_GRMZM2G087896 CARINATA_2C 44.7 ZMROD1_GRMZM2G087896 CARINATA_3B 44.4 ZMROD1_GRMZM2G087896 CARINATA_3C 44.4 ZMROD1_GRMZM2G087896 CARINATA_5B 45.8 ZMROD1_GRMZM2G087896 CARINATA_5C 44.8 ZMROD1_GRMZM2G087896 GMROD1-1 53.1 ZMROD1_GRMZM2G087896 GMROD1-2 49 ZMROD1_GRMZM2G087896 LUPDCT1 49 ZMROD1_GRMZM2G087896 LUPDCT2 48.6 ZMROD1_GRMZM2G087896 NAPUS_1A 43 ZMROD1_GRMZM2G087896 NAPUS_1C 43 ZMROD1_GRMZM2G087896 NAPUS_2A 44 ZMROD1_GRMZM2G087896 NAPUS_2C 43.6 ZMROD1_GRMZM2G087896 NAPUS_3A 43.8 ZMROD1_GRMZM2G087896 NAPUS_3C 44.4 ZMROD1_GRMZM2G087896 NAPUS_5A 45.6 ZMROD1_GRMZM2G087896 NAPUS_5C 44.8 ZMROD1_GRMZM2G087896 OSROD1_SEQIDNO11 68.9 ZMROD1_GRMZM2G087896 RCPDCT 48.2 ZMROD1_GRMZM2G087896 RCROD1_SEQIDNO9 48.2 ZMROD1_GRMZM2G087896 ZMROD1_GRMZM2G015040 83.9 ZMROD1_GRMZM2G087896 ZMROD1_GRMZM2G087896 100

    TABLE-US-00012 TABLE 7 Average fatty acid composition (%) in different lipid classes from immature seeds 16:0 18:0 18:1 18:2 GLA 18:3 SDA 20:0 20:1 20:2 DGLA 22:1 TAG 1C 10.3 5.4 37.0 13.6 4.9 6.4 1.0 2.1 15.3 0.4 1.7 1.6 2C 9.9 5.8 40.3 10.8 5.7 5.3 0.7 1.9 15.0 0.3 2.4 1.4 CK 10.6 4.9 36.8 17.6 1.7 6.7 0.1 2.0 15.0 0.5 1.8 1.5 mutant CK WT 9.4 4.7 19.5 22.0 11.6 7.9 1.6 2.2 16.6 1.2 1.2 1.4 WT 8.8 4.4 22.2 31.2 0.0 11.3 0.0 2.2 16.6 1.5 0.0 1.6 Rod mut 10.2 4.5 31.4 20.9 0.0 10.2 0.0 2.6 16.7 0.7 0.0 2.2 PC 1C 22.9 2.4 3.8 40.3 5.4 21.7 1.2 0.0 0.2 0.8 0.7 0.1 2C 20.8 2.2 3.6 40.8 7.9 21.6 1.1 0.0 0.3 0.9 0.4 0.2 CK 18.1 1.8 3.4 46.7 2.7 25.3 0.3 0.0 0.2 0.9 0.1 0.2 mutant CK WT 27.3 3.6 4.0 40.0 3.9 18.3 0.5 0.0 0.7 0.9 0.2 0.0 WT 22.6 2.6 5.8 45.9 0.0 20.6 0.0 0.0 1.1 0.9 0.0 0.0 Rod mut 18.2 2.0 2.8 48.2 0.0 27.5 0.0 0.0 0.0 0.9 0.0 0.0 DAG 1C 16.5 8.7 28.4 17.8 3.7 7.0 0.0 4.4 7.6 0.0 1.1 4.8 2C 15.5 8.2 32.5 11.5 6.0 6.1 0.0 3.9 10.0 0.0 2.2 4.0 CK 19.9 10.3 32.9 16.2 1.1 5.0 0.0 3.2 10.4 0.0 0.0 0.8 mutant CK WT 17.9 5.2 13.6 35.6 7.8 10.9 0.0 1.7 5.1 0.0 0.8 1.4 WT 17.1 7.2 24.4 34.1 0.0 5.9 0.0 2.4 6.7 0.0 0.0 2.2 Rod mut 18.2 9.7 25.1 19.2 0.0 7.3 0.0 5.2 9.9 0.0 0.0 5.4

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