ZIKA VIRUS VACCINE

Abstract

A recombinant vesicular stomatitits virus (rVSV) having a Zika virus (ZIKV) envelope (E) gene, a prime boost immunization combination against ZIKV including: (a) a prime vaccine or immunogenic composition comprising a recombinant vesicular stomatitis virus (rVSV) carrying a ZIKV envelope (E) protein, and (b) a boost vaccine or immunogenic composition comprising a rVSV carrying the same ZIKV E protein. The ZIKV gene can be genetically modified to encode a modified ZIKV E protein that elevates glycoprotein synthesis and trigger efficient humoral immune response.

Claims

1. A recombinant vesicular stomatitis virus (rVSV) carrying a gene that encodes a Zika virus (ZIKV) envelope (E) protein or that encodes for a modified ZIKV E protein.

2. The rVSV of claim 1, wherein rVSV further carries a gene that encodes a precursor to the membrane (PrM) protein.

3. The rVSV of claim 1, wherein the rVSV further carries a gene that encodes a ZIKV membrane (M) gene.

4. The rVSV of claim 1, wherein the gene encodes for the modified ZIKV E protein, the modified ZIKV E.

5. The rVSV of claim 4, wherein the modified ZIKV E protein includes a VSV G protein transmembrane domain and cytoplasmic tail (Gtc) at the C-terminus of the ZIKV E protein.

6. The rVSV of claim 4, wherein the modified ZIKV E protein includes a glycoprotein signal peptide at the N-terminus of the ZIKV E protein and a VSV G protein transmembrane domain and cytoplasmic tail (Gtc) at the C-terminus of the ZIKV E protein.

7. The rVSV of claim 6, wherein the signal peptide is a honeybee melittin signal peptide.

8. The rVSV of claim 1, wherein the rVSV is of Indiana (VSV.sub.Ind) serotype.

9. The rVSV of claim 8, wherein the rVSV.sub.Ind carries a mutant matrix protein (M), and wherein the mutant M protein includes a GML mutation (rVSV.sub.Ind-GML).

10. The rVSV of claim 1, wherein the rVSV is of New Jersey (VSV.sub.NJ) serotype.

11. The rVSV of claim 10, wherein the rVSV.sub.NJ carries a mutant matrix (M) protein, and wherein the mutant M protein includes a GMM mutation (rVSV.sub.NJ-GMM) or a GMML mutation (rVSV.sub.NJ-GMML).

12. A prime boost immunization combination against Zika virus (ZIKV) including: (a) a prime vaccine or immunogenic composition comprising a recombinant vesicular stomatitis virus (rVSV) carrying a gene that encodes for ZIKV envelope (E) protein or that encodes for a modified ZIKV E protein, and (b) a boost vaccine or immunogenic composition comprising a rVSV carrying the same gene.

13. The prime boost immunization combination against ZIKV of claim 12, wherein the rVSV of (a) and the rVSV of (b) further carry a gene that encodes for a precursor to the membrane (PrM) protein.

14. The prime boost immunization combination against ZIKV of claim 12, wherein the rVSV of (a) and the rVSV of (b) further carry a gene that encodes for a ZIKV membrane (M) protein.

15. The prime boost immunization combination against ZIKV of claim 12, wherein the gene of the rVSV of the prime vaccine or immunogenic composition and the gene of the rVSV of the boost vaccine or immunogenic composition encode for the modified ZIKV E protein, the modified ZIKV E protein having a glycoprotein signal peptide at the N-terminus of the ZIKV E protein and a VSV G protein transmembrane domain and cytoplasmic tail (Gtc) at the C-terminus of the ZIKV E protein.

16. The prime boost immunization combination against ZIKV of claim 15, wherein the glycoprotein signal peptide is a honeybee melittin signal peptide.

17. The prime boost immunization combination against ZIKV of claim 12, wherein the gene of the rVSV of the prime vaccine or immunogenic composition and the gene of the rVSV of the boost vaccine or immunogenic composition encode for the modified ZIKV E protein, the modified ZIKV protein having a VSV G protein transmembrane domain and cytoplasmic tail (Gtc) at the C-terminus of the ZIKV E protein.

18. The prime boost immunization combination against ZIKV of claim 12, wherein the rVSV of the prime vaccine or immunogenic composition and the rVSV of the boost vaccine or immunogenic composition are of Indiana serotype (rVSV.sub.Ind), or the rVSV of the prime vaccine or immunogenic composition and the rVSV of the boost vaccine or immunogenic composition are of New Jersey serotype (rVSV.sub.NJ).

19. The prime boost immunization combination against ZIKV of claim 12, wherein the rVSV of the prime vaccine or immunogenic composition is Indiana (VSV.sub.Ind) and the rVSV of the boost vaccine or immunogenic composition is New Jersey (VSV.sub.NJ).

20. The prime boost immunization combination against ZIKV of claim 12, wherein the rVSV of the prime vaccine or immunogenic composition and the rVSV of the boost vaccine or immunogenic composition carry a mutant matrix (M) protein, and wherein when the rVSV is rVSV.sub.Ind the mutant M protein includes a GML mutation (rVSV.sub.Ind-GML), and when the rVSV is rVSV New Jersey serotype the mutant M protein includes a GMM mutation (rVSV.sub.NJ-GMM) or a GMML mutation (rVSV.sub.NJ-GMML).

21. A method for inducing an immune response in a mammal against Zika virus (ZIKV), comprising: (a) administering to the mammal an effective amount of a prime vaccine or immunogenic composition including a rVSV carrying a gene that encodes for a ZIKV envelope (E) protein or that encodes for a modified ZIKV E protein, and (b) administering to the subject a booster vaccine or immunogenic composition comprising a rVSV carrying the same gene.

22. The method for inducing an immune response in a mammal against ZIKV of claim 21, wherein the rVSV of (a) and the rVSV of (b) further carry a gene that encodes for a precursor to the membrane (PrM) protein.

23. The method for inducing an immune response in a mammal against ZIKV of claim 21, wherein the rVSV of (a) and the rVSV of (b) further carry a gene that encodes for a ZIKV membrane (M) protein.

24. The method for inducing an immune response in a mammal against ZIKV of claim 21, wherein the rVSV of the prime vaccine or immunogenic composition and the rVSV of the boost vaccine or immunogenic composition include the gene that encodes for the modified ZIKV E protein, the modified ZIKV E protein having a glycoprotein signal peptide at the N-terminus of the ZIKV E protein and a VSV G protein transmembrane domain and cytoplasmic tail (Gtc) at the C-terminus of the ZIKV E protein.

25. The method for inducing an immune response in a mammal against ZIKV of claim 24, wherein the glycoprotein signal peptide is a honeybee melittin signal peptide.

26. The method for inducing an immune response in a mammal against ZIKV of claim 21, wherein the ZIKV E protein includes a VSV G protein transmembrane domain and cytoplasmic tail (Gtc) at its C-terminus.

27. The method of claim 21, wherein the rVSV of the prime vaccine or immunogenic composition is Indiana (rVSV.sub.Ind) and the rVSV of the boost vaccine or immunogenic composition is rVSV.sub.Ind, or the rVSV of the prime vaccine or immunogenic composition is New Jersey (rVSV.sub.NJ) and the rVSV of the boost vaccine or immunogenic composition is rVSV.sub.NJ.

28. The method of claim 21, wherein the rVSV of the prime vaccine or immunogenic composition is Indiana (rVSV.sub.Ind) and the rVSV of the boost vaccine or immunogenic composition is New Jersey (rVSV.sub.NJ).

29. The method of claim 21, wherein the rVSV of the prime vaccine or immunogenic composition and the rVSV of the boost vaccine or immunogenic composition include a mutant matrix (M) protein gene.

30. The method of claim 29, wherein the rVSV is of Indiana serotype and the mutant M protein includes a GML mutation (rVSV.sub.Ind-GML), or the rVSV is of New Jersey serotype and the mutant M protein includes a GMM mutation (rVSV.sub.NJ-GMM) or a GMML mutation (rVSV.sub.NJ-GMML).

31. The method of claim 30, wherein the immune response includes a humoral and a cellular immune response.

32. A Zika virus (ZIKV) vaccine or immunogenic composition comprising the rVSV of claim 1.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0042] The present invention will become more fully understood from the detailed description given herein and from the accompanying drawings, which are given by way of illustration only and do not limit the intended scope of the invention.

[0043] FIG. 1. (SEQ ID NOS: 13 and 14) Generation of four different rVSV-ZIKV constructs expressing the Zika virus envelope (E) protein. ZIKV genes were inserted into pTV-VSV between G and L genes. pTV-ZIKV-PrM+E contains ZIKV PrM+E genes, pTV-ZIKV-ME contains ZIKV ME genes, pTV-ZIKV-msp-E contains ZIKV E gene with honeybee melittin signal peptides (msp; 21 amino acids in blue), and pTV-ZIKV-msp-E-Gct contains E protein with msp at the NH.sub.2-terminus (20 amino acids in green) and VSV G protein transmembrane and cytoplasmic tail (Gtc in red) at the COOH-terminus (29 amino acids in brown). 1: Leader, t: Trailer, N: Nucleocapsid, P: Phosphoprotein, M: Matrix, G: Glycoprotein, L: Large protein (RNA dependent RNA polymerase), T7P: T7 RNA polymerase promoter, HDV59: 59 nucleotides of hepatitis delta virus ribozyme.

[0044] FIGS. 2A to 2D. ZIKV E protein expression in cells infected with rVSV.sub.Ind-ZIKV and rVSV.sub.NJ-ZIKV. FIG. 2A) Detection of ZIKV E protein in infected cell lysates. FIG. 2B) Detection of ZIKV E protein in the pelleted viral particles. BHK21 cells were infected with an MOI of 6 recombinant VSV.sub.Ind-ZIKVs for 6 hours. rVSV.sub.Ind-ZIKV Infected cells were harvested and lysed. Infected cell culture supernatants were collected, and viral particles were pelleted by ultra-centrifugation through 30% sucrose cushion. The samples of infected cell lysates and pelleted viral particles were prepared and analyzed by Western Blot using an anti-ZIKV E antibody 1:3000 dilution. The rVSV.sub.Ind-GML served as the negative control. Lane 1: rVSV.sub.Ind-GML-PrM+E, Lane 2: rVSV.sub.Ind-GML-ME, Lane 3: rVSV.sub.Ind-GML-msp-E, Lane 4: rVSV.sub.Ind-GML-msp-E-Gtc, Lane 5: rVSV.sub.Ind-GML. FIG. 2C) Detection of ZIKV E protein in infected cell lysates. FIG. 2D) Detection of ZIKV E protein in the pelleted viral particles. BHK21 cells were infected with recombinant VSV.sub.NJ-ZIKVs at MOI of 6 for 6 hours. Infected cells were harvested and lysed. Infected cell culture supernatants were collected, and viral particles were pelleted by ultra-centrifugation through a 30% sucrose cushion. The samples of infected cell lysates and pelleted viral particles were prepared and analyzed by Western Blot using anti-ZIKV E antibody at 1:3000 dilution. The rVSV.sub.NJ-GMM served as the negative control. Lane 1: rVSV.sub.NJ-GMM-PrM+E, Lane 2: rVSV.sub.NJ-GMM-ME, Lane 3: rVSV.sub.NJ-GMM-msp-E, Lane 4: rVSV.sub.NJ-GMM-msp-E-Gtc, Lane 5: rVSV.sub.NJ-GMM.

[0045] FIGS. 3A to 3C. Immunogold staining with Anti-VSV G protein and Anti-ZIKV E protein. FIG. 3A) Detection of VSV G protein (blue arrow) on the recombinant VSV.sub.Ind-GML-ZIKV-msp-E-Gtc viral particles. FIG. 3B) Detection of ZIKV E protein (red arrow) on the recombinant VSV.sub.Ind-GML-ZIKV-msp-E-Gtc viral particles. FIG. 3C) Diagram of rVSV-ZIKV pseudovirion. The rVSV.sub.Ind-GML-ZIKV-msp-E-Gtc viruses were analysed by immunogold staining with anti-VSV G antibody (Kerafast) and anti-ZIKV E antibody (Kerafast), and samples were checked under a Philips CM10 electron microscope.

[0046] FIGS. 4A to 4C. Humoral Immune responses with different doses of rVSV-ZIKV. FIG. 4A) Humoral immune responses by rVSV-ZIKV-PrM+E immunization. Five mice per each group were prime and boost immunized intramuscularly at two-week intervals with PBS, live ZIKV (2×10.sup.9 PFU), rVSV-Mock or eight different doses of rVSV-ZIKV-PrM+E (from 1×10.sup.5 PFU to 5×10.sup.8 PFU) respectively. rVSV.sub.Ind serotype was used for prime-immunization, then rVSV.sub.NJ serotype was used for boost-immunization. Sera were collected from each mouse at two weeks after each immunization (Day 13 and 27). The serum IgG titer against the ZIKV E protein was analyzed by ELISA. FIG. 4B) Humoral immune responses by rVSV-ZIKV-msp-E-Gtc immunization. Five mice per each group were prime and boost immunized intramuscularly with PBS, rVSV-Mock, or eight different doses of rVSV-ZIKV-msp-E-Gtc (from 1×10.sup.5 PFU to 5×10.sup.8 PFU) at two-week intervals. rVSV.sub.Ind serotype was used for prime-immunization, then rVSV.sub.NJ serotype was used for boost-immunization. Sera were collected from each mouse at two weeks after each immunization (Day 13 and 27). The serum IgG titer against the ZIKV E protein was analyzed by ELISA. FIG. 4C) Comparative immune responses of rVSV-ZIKV-PrM+E, rVSV-ZIKV-msp-E-Gtc, rVSV-Mock, and live ZIKV. The immunizations were carried out with 5×10.sup.7 PFU and 5×10.sup.8 PFU of rVSV-ZIKV-PrM+E, rVSV-ZIKV-msp-E-Gtc, 5×10.sup.8 PFU of rVSV-Mock or 2×10.sup.9 PFU of live ZIKV as described in FIG. 4A and FIG. 4B. Statistical significance was determined by One-Way ANOVA with Tukey's correction (*, p<0.05; **, p<0.01; ***p<0.001; ****p<0.0001; ns, not significant). The data was presented as means with error bars of standard deviation. The dotted line across represents background.

[0047] FIG. 5. Evaluation of neutralizing antibody titers against live ZIKV. Five mice per group were prime and boost immunized intramuscularly with 2×10.sup.9 PFU of live ZIKV, rVSV-Mock, rVSV-ZIKV-PrM+E (5×10.sup.7 PFU and 5×10.sup.8 PFU), or rVSV-ZIKV-msp-E-Gtc (5×10.sup.7 PFU and 5×10.sup.8 PFU) at two-week intervals. Sera were collected at two weeks after the boost-immunization (Day 27). Sera diluted in 1/20, 1/40, 1/80, 1/160, and 1/320 were tested for neutralizing activity against ZIKV-Fortaleza/2015 (Brazil) strain. Serially-diluted mouse sera were incubated with ZIKV-Fortaleza/2015 (Brazil), and the mixture was added to Vero cells. Three days after the infection, plaques were counted. The decrease in the number of plaques was represented as % neutralization. Statistical significance was determined by One-Way ANOVA with Tukey's correction (*, p<0.05; ns, not significant). The data was presented as means with error bars of standard deviation.

[0048] FIG. 6. T Cell Responses Against ZIKV E Protein. Five mice per group were prime and boost immunized with 2×10.sup.9 PFU of live ZIKV, rVSV-Mock, rVSV-ZIKV-PrM+E (1×10.sup.8 PFU and 5×10.sup.8 PFU), or rVSV-ZIKV-msp-E-Gtc (1×10.sup.8 PFU and 5×10.sup.8 PFU) intramuscularly. Two weeks after the boost-immunization, the splenocytes were prepared and were stimulated with HIV-gag peptide (AMQMLKETI) (SEQ ID NO: 15) (as a control peptide, purple column), CD8-specific (blue column), or CD4-specific (red column) peptide of ZIKV E protein [Zika-E646 (GRLITANPVITESTE) (SEQ ID NO: 16) and Zika-E294 (IGVENRDFV) (SEQ ID NO: 17)]. The number of IFN-7 secreting cells was counted using the mouse IFN-7 ELISPOT kit. Statistical significance was determined by One-Way ANOVA with Tukey's correction (*, p<0.05; **, p<0.05; ns, not significant). The data was presented as means with error bars of standard deviation.

[0049] FIGS. 7A to 7C. In vivo protection against Lethal ZIKV Challenge. B6(Cg)-Ifnar 1.sup.tm1.2Ees/J (Ifnar.sup.−/−) mice were prime and boost immunized with 5×10.sup.8 PFU of rVSV-Mock, rVSV-ZIKV-PrM+E, or rVSV-ZIKV-msp-E-Gtc respectively via intramuscular route at two-week intervals. Mice were challenged intraperitoneally with 1×10.sup.3 PFU of ZIKV-Fortaleza/2015 (Brazil) at two weeks after boost-immunization. The losses of body weight and survival were monitored daily for 14 days after the challenge. FIG. 7A) Survival rate of Ifnar.sup.−/− mice immunized with rVSV-Mock, rVSV-ZIKV-PrM+E, rVSV-ZIKV-msp-E-Gtc after challenge with ZIKV-Fortaleza/2015 (Brazil). The survival rate in challenged groups was compared by Log-rank test. The p value was 0.0029. FIG. 7B) Loss of body weight of rVSV-Mock, rVSV-ZIKV-PrM+E, rVSV-ZIKV-msp-E-Gtc immunized Ifnar.sup.−/− mice after ZIKV-Fortaleza/2015 (Brazil) challenge. Body weight is presented as means of percentage of initial body weight with error bars of standard deviation. FIG. 7C) Experimental scheme of in vivo protection study using Ifnar.sup.−/− mice.

DETAILED DESCRIPTION OF THE INVENTION

1. Definitions

[0050] For convenience, the meaning of certain terms and phrases employed in the specification, examples, and appended claims are provided below. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

[0051] The articles “a” and “an” are used herein to refer to one or more than one (i.e., to at least one) of the grammatical object of the article.

[0052] The terms “animal” and “subject” as used herein includes all members of the animal kingdom including mammals, preferably humans.

[0053] The term “effective amount” as used herein means an amount effective and at dosages and for periods of time necessary to achieve the desired result.

[0054] “rVSV” is used to refer to a recombinant vesicular stomatitis virus.

[0055] The term “Indiana”, and “IND” are used to refer to the VSV serotype Indiana (VSV.sub.Ind). The term “New Jersey”, and “NJ” are used to refer to the VSV serotype New Jersey (VSV.sub.NJ).

[0056] “M.sub.WT” “M(WT)” are used to refer to VSV having a wild type M gene.

[0057] “G22E” is used to refer to an M.sub.WT gene in VSV.sub.NJ having a glycine changed to a glutamic acid at position 22.

[0058] “G21E” is used to refer to an M.sub.WT gene in VSV.sub.Ind having a glycine changed to a glutamic acid at position 21.

[0059] “L110A” is used to refer to an M.sub.WT gene in VSV.sub.NJ having a leucine changed to alanine at position 110.

[0060] “L111A” is used to refer to an M.sub.WT gene in VSV.sub.Ind having a leucine changed to alanine at position 111.

[0061] “L110F” is used to refer to an M.sub.WT gene in VSV.sub.NJ having a leucine changed to phenylalanine at position 110.

[0062] “L111F” is used to refer to an M.sub.WT gene in VSV.sub.Ind having a leucine changed to phenylalanine at position 111.

[0063] “M51R” is used to refer to an M.sub.WT gene in the VSV.sub.Ind having a methionine changed to an arginine at position 51.

[0064] “M48R+M51R” or “M48R/M51R” are used to refer to an M.sub.WT gene in VSV.sub.NJ having a methionine changed to an arginine at positions 48 and 51 respectively.

[0065] “rVSV.sub.Ind(GML)” is used to refer to an M.sub.WT gene in VSV.sub.Ind having the combined mutation G21E, M51R and one of L111A or L111F.

[0066] “rVSV.sub.NJ(GMM)” is used to refer to an M.sub.WT gene in VSV.sub.NJ having the combined mutation G22E, M48R/M51R.

[0067] “rVSV.sub.NJ(GMML)” is used to refer to an M.sub.WT gene in VSV.sub.NJ having the combined mutation G22E, M48R/M51R and one of L110A or L110F.

[0068] Although in the case of rVSV.sub.Ind either L111A or L111F, and in the case of rVSV.sub.NJ either L110A or L110F can be used, for improved stability, alanine (A) is preferred over phenylalanine (F).

[0069] The term “protein” as used herein is defined as a chain of amino acid residues, usually having a defined sequence. As used herein the term protein is inclusive of the terms “peptides” and “proteins”. The terms also encompass an amino acid polymer that has been modified.

2. Overview

[0070] The present invention features recombinant vesicular stomatitis viruses (rVSV), immunization platforms, immunization regimens and medicaments useful for inducing an immune response in a subject and preventing Zika virus (ZIKV) infection in the subject.

[0071] The rVSVs of the present invention feature a ZIKV envelope (E) gene.

[0072] The ZIKV gene can be genetically modified to encode a modified ZIKV E protein that elevates glycoprotein synthesis and triggers efficient humoral immune response. In one embodiment, the ZTKV E gene is genetically modified to produce ZIKV E proteins having a glycoprotein signal peptide at its N-terminus. Any glycoprotein signal peptide that allows the E protein to be glycosylated and involved in intracellular trafficking can be used, for example the honeybee melittin signal peptide. For example, the E gene is genetically modified to produce E proteins having a honeybee melittin signal peptide (msp) at its N-terminus or to produce E proteins having the msp at its N-terminus, and the transmembrane domain and cytoplasmic tail of the VSV glycoprotein (Gtc) to form pseudotype VSVs that trigger efficient humoral immune responses against the ZTKV E protein.

[0073] In another embodiment, the rVSV of the present invention also carries a PrM gene or M gene.

[0074] The platforms, regimens and medicaments comprise: (a) one vaccine or immunogenic composition comprising a recombinant vesicular stomatitis virus (rVSV) carrying a gene that encodes for a ZIKV envelope (E) protein or for a modified ZTKV E protein, and (b) another vaccine or immunogenic composition comprising a rVSV carrying the same gene that encodes for the ZIKV E protein or the modified ZIKV E protein. In one aspect, the E protein has a msp attached to its N-terminus.

[0075] In another embodiment, the present invention is a ZIKV E protein having a msp attached at the N-terminus of the ZIKV E protein.

[0076] In another embodiment, the present invention is a ZIKV E protein having a msp attached at the N-terminus of the ZTKV E protein and a transmembrane domain and cytoplasmic tail of the VSV glycoprotein (Gtc) at the C-terminus of the ZIKV E protein.

[0077] In another embodiment, the present invention is a ZIKV E protein having a transmembrane domain and cytoplasmic tail of the VSV glycoprotein (Gtc) at the C-terminus of the ZIKV E protein.

[0078] In aspects, the genes of the rVSVs of the present disclosure (including the VSV genes, mutated VSV genes and the genes that encode for ZIKV E protein, ZIKV M protein and ZTKV PrM protein and modifications thereof), including the rVSVs included in the vaccines or immunogenic compositions of the present disclosure, are codon optimized for expression in a mammalian cell, including a human cell.

3. Vaccines or Immunogenic Compositions of the Invention

[0079] The present invention further features vaccines or immunogenic compositions comprising an rVSV as described above. The vaccine or immunogenic compositions of the invention are suitable for administration to subjects in a biologically compatible form in vivo. The expression “biologically compatible form suitable for administration in vivo” as used herein means a form of the substance to be administered in which any toxic effects are outweighed by the therapeutic effects. The substances maybe administered to any animal or subject, preferably humans. The vaccines of the present invention may be provided as a lyophilized preparation. The vaccines of the present invention may also be provided as a solution that can be frozen for transportation. Additionally, the vaccines may contain suitable preservatives such as human albumin, bovine albumin, sucrose, glycerol or may be formulated without preservatives. If appropriate (i.e., no damage to the VSV in the vaccine), the vaccines may also contain suitable diluents, adjuvants and/or carriers.

[0080] A ZIKV vaccine or immunogenic composition according to this disclosure comprises a rVSV of the present disclosure.

[0081] In another embodiment of the present disclosure provides for a prime boost immunization combination against Zika virus (ZTKV) including: (a) a prime vaccine or immunogenic composition comprising a recombinant vesicular stomatitis virus (rVSV) carrying a gene that encodes for ZTKV envelope (E) protein or that encodes for a modified ZTKV E protein, and (b) a boost vaccine or immunogenic composition comprising a rVSV carrying the same gene.

[0082] In embodiments, the rVSV of the prime vaccine or immunogenic composition and the rVSV of the boost vaccine or immunogenic composition also carry a ZIKV PrM gene and/or a ZIKV M gene.

[0083] The rVSV of the prime vaccine or immunological composition may be of the same or different serotype as the rVSV of the boost vaccine or immunological composition. For example, both the prime and boost vaccines or immunogenic compositions are rVSV.sub.Ind; or both the prime and boost vaccines or immunogenic compositions are rVSV.sub.NJ; or the rVSV of the prime vaccine or immunogenic composition is rVSV.sub.Ind and the rVSV of the boost vaccine or immunogenic composition is rVSV.sub.NJ; or the rVSV of the prime vaccine or immunogenic composition is rVSV.sub.NJ and the rVSV of the boost vaccine or immunogenic composition is rVSV.sub.Ind.

[0084] The dose of the vaccine may vary according to factors such as the disease state, age, sex, and weight of the individual, and the ability of antibody to elicit a desired response in the individual. Dosage regime may be adjusted to provide the optimum therapeutic response. The dose of the vaccine may also be varied to provide optimum preventative dose response depending upon the circumstances.

4. Methods of Use

[0085] The present invention also features methods of inducing an immune response in a subject against ZIKV and/or preventing or treating a ZIKV infection in a subject comprising administering to the subject an effective amount of a combination of a vaccine or immunogenic composition of the present invention.

[0086] The present invention provides for a method for inducing an immune response in a subject to a ZIKV characterized in that said method comprises the following steps: (a) administering to the subject an effective amount of a prime vaccine or immunogenic composition including a rVSV carrying a ZIKV envelope (E) gene that encodes for a ZIKV E protein or that encodes for a modified ZICV E protein, and (b) administering to the subject a boost vaccine or immunogenic composition comprising a rVSV carrying the same ZIKV E gene that encodes the same ZIKV E protein or modified ZIKV E protein.

[0087] The rVSV of the prime vaccine or immunogenic composition may be of the same or different serotype as the rVSV of the booster vaccine or immunogenic composition. For example, both the prime and boost vaccines or immunogenic compositions are rVSV.sub.Ind; or both the prime and boost vaccines or immunogenic compositions are rVSV.sub.NJ; or the rVSV of the prime vaccine or immunogenic composition is rVSV.sub.Ind and the rVSV of the boost vaccine or immunogenic composition is rVSV.sub.NJ; or the rVSV of the prime vaccine or immunogenic composition is rVSV.sub.NJ and the rVSV of the boost vaccine or immunogenic composition is rVSV.sub.Ind.

[0088] The ZIKV E gene, in one embodiment, includes one of the genetically modified ZIKV E genes described above.

[0089] In aspects of the invention the methods for inducing an immune response in a mammal to a ZIKV and the methods for preventing or treating an infection caused by ZIKV may further comprise step (c) administering to the subject an effective amount of the vaccine or immunogenic composition of either step (a) or step (b). Step (c) may be administered to the subject more than one time over the course of inducing an immune response, preventing or treating.

[0090] Advantages

[0091] Advantages of the recombinant VSV-based platform technology of the present invention are first, a highly efficient prime-boost vaccination can be achieved with two antigenically distinct serotypes of rVSV vectors, because the vector immunity against the priming Indiana serotype (VSV.sub.Ind) will not neutralize the boosting New Jersey serotype (VSV.sub.NJ) vector. Thus, VSV.sub.NJ carrying the same gene of interest as rVSV.sub.Ind will provide maximum boost effects. A highly efficient prime-boost vaccination can also be achieved with the same serotype of rVSV vectors (i.e., both the prime and boost are rVSV.sub.Ind or both the prime and boost are rVSV.sub.NJ), because the pseudotype VSVs will carry both VSV G protein and ZIKV E protein on the surface. This pseudotype VSV can use either VSV receptor or ZIKV receptor to infect cells. Second, our genetically modified VSV.sub.Ind M gene mutant (rVSV.sub.Ind-GML) and genetically modified VSV.sub.NJ M gene mutant (rVSV.sub.NJ-GMM or GMML mutation (rVSV.sub.NJ-GMML) vectors are completely safe, attenuated temperature-sensitive mutants [22]. Third, rVSV.sub.Ind-GML, rVSV.sub.NJ-GMM and rVSV.sub.NJ-GMML vectors carrying foreign genes replicate highly efficiently. Therefore, high titer rVSV-based vaccines are relatively easy to prepare. Fourth, rVSV.sub.Ind-GML, rVSV.sub.NJ-GMM and rVSV.sub.NJ-GMML vectors can accommodate a large-size foreign gene with up to 6,000 nucleotides, without decreasing the virus titer [24], and finally both serotypes of VSV have a very wide host range including humans.

[0092] The above disclosure generally describes the present invention. A more complete understanding can be obtained by reference to the following specific Examples. These Examples are described solely for purposes of illustration and are not intended to limit the scope of the invention. Changes in form and substitution of equivalents are contemplated as circumstances may suggest or render expedient. Although specific terms have been employed herein, such terms are intended in a descriptive sense and not for purposes of limitation.

Examples

[0093] The examples are described for the purposes of illustration and are not intended to limit the scope of the invention.

[0094] We have employed a genetically modified dual serotype of vesicular stomatitis virus (VSV) platform technology [22, 23] to develop a prime-boost vaccine against ZIKV.

[0095] We have constructed rVSV carrying a modified ZIKV envelope (E) protein gene using genetically modified attenuated strains of both VSV.sub.Ind and VSV.sub.NJ as a prime-boost rVSV-ZIKV vaccine. We found prime immunization with rVSV.sub.Ind carrying the ZIKV E gene with honeybee melittin signal peptide (msp) at the N-terminus and VSV G protein transmembrane domain and cytoplasmic tail (Gtc) at the C-terminus of the E gene followed by boost immunization with rVSV.sub.NJ carrying the same modified E gene induced strong protective immune responses.

[0096] Here we present the induction of protective immune responses upon prime-boost vaccination of antigenically distinct dual serotypes of recombinant VSV vectors carrying a genetically modified E protein gene of ZIKV.

[0097] Methods

[0098] 1 Cells and Viruses

[0099] BHK21 cells obtained from the American Type Culture Collection (ATCC) were cultured in Dulbecco's Modified Eagle Medium (DMEM) containing 5% fetal bovine serum (FBS). The BHK cell line expressing T7 bacteriophage RNA polymerase, BSR T7/5 cells, was provided by Drs. Buchholz and Conzelmann (Federal Research Center for Virus Diseases of Animals, Germany) [25] and cultured in DMEM containing 5% FBS and 500 μg/ml Geneticin (Gibco). Recombinant VSV-ZIKVs recovered from reverse genetics were purified three times by plaque picking and amplified in BHK21 cells as we have described previously [22].

[0100] ZIKV strain Fortaleza 2015 (ZIKV-Fortaleza/2015, Brazil) [26] was kindly provided by the Walter Reed Army Institute of Research (WRAIR). Vero cells (ATCC, CCL-81) were used to propagate the ZIKV. ZIKV was infected to Vero cells at the multiplicity of infection (MOI) of 0.1˜ 1 and harvested after three to four days post infection. The culture medium containing viruses was centrifuged at 5,000 rpm at 4° C. for 30 minutes to remove cell debris. The supernatant containing viruses was aliquoted and stored at −80° C. Plaque assay was performed to confirm the virus titer.

[0101] 2 Plasmids Construction

[0102] The full-length cDNA clone of VSV.sub.Ind-GML and VSV.sub.NJ-GMM was cloned into the transcription vector pTV and named as pTV-VSV.sub.Ind-GML and pTV-VSV.sub.NJ-GMM as described previously [22]. To clone the ZIKV genes into the VSV vectors, the cDNA of ZIKV strain H/PH/2013 (European virus archive, EVAG) was used as the template.

[0103] For PrME gene cloning; the full length PrME gene was amplified by PCR using primer PrME-F and PrME-R (Table 1). The PCR product was cloned into the pBKS vector first, and after confirming the sequences, the correct PrME gene was cut out from the pBKS and inserted into pTV-VSV.sub.Ind-GML and pTV-VSV.sub.NJ-GMM between G and L gene using PmeI and MluI restriction sites. For ME gene cloning; the precursor to the membrane (Pr) gene was not included, only the full-length ME gene was amplified by the PCR using primer ME-F and PrME-R (Table 1). The PCR product was clone into pBKS vector first, and after confirming the sequences, the correct ME gene was cut out from pBKS and inserted into pTV-VSV.sub.Ind-GML and pTV-VSV.sub.NJ-GMM between the G and L genes using the PmeI and MluI restriction sites. For E gene with honeybee melittin signal peptide (msp) cloning; the signal peptide of E gene was replaced with msp. The msp gene (63 bps) was amplified by PCR using primer E-F and Emsp-mega (Table 1), and then the msp and the PCR product was used as a forward primer and primer PrME-R together to amplify the E gene. The PCR product was cloned into pBKS vector, and after the sequence were confirmed, the correct E gene with msp gene was cut out from pBKS and inserted into pTV-VSV.sub.Ind-GML and pTV-VSV.sub.NJ-GMM between G and L gene using PmeI and MluI restriction sites. For E gene with honeybee melittin signal peptide (msp) and VSV.sub.Ind G protein transmembrane domain and cytoplasmic tail (Gtc) cloning; the signal peptide of E gene was replaced with msp gene and the transmembrane domain of E gene was replaced with Gtc. First, the msp gene was amplified by PCR using primer E-F and Emsp-mega (Table 1); second, the VSV.sub.Ind Gtc was amplified using EGtc mega and EGtc R (Table 1) and then the PCR product of msp and Gtc were used as primers to amplify the E gene. The PCR product was cloned into the pBKS vector, and after confirming the sequences, the correct E gene with msp and Gtc was cut out from the pBKS and inserted into the pTV-VSV.sub.Ind-GML and pTV-VSV.sub.NJ-GMM between the G and L genes using PmeI and MluI restriction sites.

[0104] 4 Western Blot Analysis

[0105] To prepare infected cell lysates, BHK, cells were infected with recombinant VSV-ZIKVs at an MOI of 6. At 6 hours post-infection, infected cells were collected and lysed in 100 μl of lysis buffer. To prepare samples of pelleted recombinant VSV-ZIKV particles, BHK.sub.21 cells were infected with recombinant VSV-ZIKVs at an MOI of 0.1. At 20 hours post-infection, cell culture supernatants were collected and ultra-centrifuged through a 30% sucrose cushion at 35,000 rpm (Beckman SW-41) at 4° C. for 1 hour. The virus pellet was resuspended in 100 μl lysis buffer and stored at −80° C.

[0106] The standardized samples were analyzed by SDS-PAGE and detected by using the ECL Western blotting analysis kit (GE Healthcare). To detect ZIKV E protein, the mouse monoclonal anti-Zika E protein antibody (BioFront) (1:3,000) was used.

[0107] 5 Immune Electron Microscopy

[0108] BHK21 cells were infected with rVSV.sub.Ind-GML-ZIKV-msp-E-Gtc at an MOI of 0.1. At 20 hours post-infection, cell culture supernatants were collected and fixed with 0.1% glutaraldehyde (Merck), and then ultra-centrifuged through a 30% sucrose cushion at 35,000 rpm (Beckman SW-41) at 4° C. for 1 hour. The fixed virus pellet was resuspended in 400 μl TNE buffer and stored at 4° C. The virus surface proteins were labelled with immune-gold conjugated antibodies and analyzed by the Philips CM10 electron microscopy. To detect the Zika virus E protein, the mouse monoclonal anti-Zika E protein antibody 3E10D1 (Kerafast) (1:10) and goat anti-mouse IgG 15 nm gold conjugated antibody (EMS) were used. To detect VSV G protein, anti-VSV-G antibody 8G5F11 (Kerafast) (1:10) and goat anti-mouse IgG 15 nm gold conjugated antibody (EMS) were used.

[0109] 6 Mice

[0110] Six-week-old C57BL/6 mice were purchased from Koatech (Pyeongtaek, Korea). Five to seven-week-old B6(Cg)-Ifnar 1.sup.tm1.2Ees/J (Ifnar.sup.−/−, JAX stock #028288) mice were purchased from Jackson Laboratories (Bar Harbor, Me., USA). Prior to the start of each animal experiment, all mice had an acclimation period of one week. All procedures were approved by the Institutional Animal Care and Use Committee at the International Vaccine Institute (IACUC Approval #2018-009).

[0111] 7. Immunization

[0112] In all animal experiments, priming immunization was performed with rVSV.sub.Ind serotype. Then two weeks after priming, boosting immunization was performed with rVSV.sub.NJ serotype. Two weeks after each immunization, blood was collected from the retro-orbital plexus, and serum was obtained by centrifugation. The sera were stored at −80° C. until further analysis. It should be understood that boosting immunization may be performed at any time after the priming, including, without limitations, days, or weeks after priming.

[0113] For dose determination test, C57BL/6 mice were immunized with rVSV-ZIKV-PrM+E and rVSV-ZIKV-msp-E-Gtc constructs with 8 different doses from 1×10.sup.5 PFU to 5×10.sup.8 PFU, 2×10.sup.9 PFU of live ZIKV, 1 μg of animal cell-expressed ZIKV E protein or 5×10.sup.8 PFU of rVSV-Mock and PBS as a negative control via intramuscular injection. For the immunogenicity test, C57BL/6 mice were immunized with 5×10.sup.7 PFU or 5×10.sup.8 PFU of rVSV-ZIKV-PrM+E, and rVSV-ZIKV-msp-E-Gtc constructs, 2×10.sup.9 PFU of live ZIKV for positive control or 5×10.sup.8 PFU of rVSV-Mock as negative control through intramuscular injection.

[0114] 8 Live ZIKV Challenge Protection Experiment

[0115] For live ZIKV challenge protection experiment, B6(Cg)-Ifnar 1.sup.tm1.2Ees/J (Ifnar.sup.−/−) mice were immunized with 5×10.sup.8 PFU of rVSV-ZIKV-PrM+E, rVSV-ZIKV-msp-E-Gtc or rVSV-Mock.

[0116] Two weeks after boost immunization, the mice were challenged with 1×10.sup.3 PFU of live ZIKV (ZIKV-Fortaleza/2015, Brazil) by intraperitoneal route. Body weight loss and survival rate were monitored every day for 14 days.

[0117] 9 ELISA

[0118] To determine the ZIKV E protein-specific antibody titer, 96-well plates (Thermo Fisher Scientific, Waltham, Mass., USA) were coated with 2 μg/ml of ZIKV E protein (Cat #MBS319787, MyBioSource, San Diego, Calif., USA) in a 50 mM NaHCO.sub.3 buffer for 16 hours at 4° C. After blocking with the blocking buffer [1% BSA (Merck, Darmstadt, Germany) in PBS] for 1 hour at 37° C., serum samples from mice were added into the plate as a 5-fold serial dilution, starting with a 1:30 dilution in the blocking buffer and incubated at 37° C. for 1 hour. Then, 1:3000 diluted HRP-conjugated goat anti-mouse IgG (Southern Biotech, Birmingham, Ala., USA) was applied and incubated at 37° C. for 1 hour. After washing, peroxidase substrate (TMB) solution (Millipore, Billerica, Mass., USA) was added to the each well and incubated within 10 minutes at room temperature (RT). The color development was stopped by adding 0.5N HCl (Merck, Darmstadt, Germany) and the optical density (OD) values were measured at wavelength 450 nm using the ELISA reader (Molecular Devices, San Jose, Calif., USA). The antibody titer was expressed as a reciprocal log 2 titer of serum dilution showing OD value of 0.2.

[0119] 10 Plaque Reduction Neutralization Assay

[0120] ZIKV-specific neutralizing activity of the sera was analyzed by using the plaque reduction neutralization test (PRNT). Heat-treated mice sera were serially two-fold diluted with Eagle's Minimum Essential Medium (EMEM; containing 10% FBS). The diluted sera were incubated with 100 PFUs of ZIKV (ZIKV-Fortaleza/2015, Brazil) at 37° C. for 1 hour. Virus-sera mixtures were added to the Vero cells and incubated at 37° C. for 30 min. After incubation, the supernatant was removed and replaced with an agar overlay media containing 1% (w/v) low-melting agarose in EMEM (containing 2% FBS). After 3 days, the plates were fixed with 4% (v/v) formaldehyde solution (Sigma, Darmstadt, Germany). Then, plates were stained with 1% crystal violet solution (Sigma, Darmstadt, Germany) at RT for 1 hour and rinsed with water. Plaque numbers were counted and the neutralizing antibodies titer was determined as the serum dilution that inhibited 50% of the tested virus inoculum.

[0121] 11 ELISpot Assay

[0122] To measure T cell responses after vaccination, an IFN-7 ELISpot assay was carried out using the Mouse IFN-7 ELISPOT Kit (BD Biosciences, San Jose, Calif., USA) according to the manufacturer's instructions. Briefly, spleens were removed from each mouse at two weeks after boost immunization, and then cellular suspensions of splenocytes were obtained by passing spleens through a 70 μm cell strainer (BD Bioscience, San Jose, Calif., USA). Red blood cells (RBCs) were removed by hemolysis using ACK Lysing Buffer (Thermo Fisher Scientific, Waltham, Mass., USA). 2×10.sup.5 cells were added into pre-coated BD™ ELISPOT plates in presence or absence of stimuli for 16 hours at 37° C. in 5% CO.sub.2 incubator. After stimulation, cells and peptide stimulants were discarded and each well was soaked in distilled water for 3-5 minutes. After washing three times with Washing Buffer (BD Bioscience, San Jose, Calif., USA), 100 μl of diluted detection antibody [Biotinylated anti-mouse IFN-7 (BD Bioscience, San Jose, Calif., USA)] in Assay Diluent (BD Biosciences, San Jose, Calif., USA) was added to each well and incubated for 2 hours at RT. After washing, 1:100 diluted streptavidin-HRP (BD Bioscience, San Jose, Calif., USA) in Assay Diluent was added to each well, and then plates were incubated within 1 hour at RT. The plates were washed with Wash Buffer four times, and then washed with PBS twice. After washing, 1:50 diluted AEC chromogen with AEC Substrate Buffer (BD Bioscience, San Jose, Calif., USA) was added to each well. The spot development was stopped by washing with distilled water in each well. Air-dried plates were used to count the number of spots. The spots were counted using an ELISPOT reader (Molecular devices, San Jose, Calif., USA)

[0123] 12. Statistics

[0124] The data on graphs were presented as means with error bars of standard deviation (SD). Statistical differences between mice groups were determined by one-way analysis of variance (ANOVA) with Tukey's correction for multiple comparisons. GraphPad Prism 8.3 was used to do the statistical analysis. Probability levels<0.05 (*, p<0.05; **, p<0.01; ***p<0.001; ****p<0.0001) were indicative of statistical significance.

[0125] Results

[0126] 1. Construction of VSV-ZIKV E Recombinant Viruses

[0127] Humoral immune response against the ZIKV envelope (E) protein is believed to induce the virus neutralizing and protective antibodies. Thus, the E protein has been regarded as the primary target for ZIKV vaccine development [16]. We have chosen four different forms of the ZIKV E protein gene in order to determine which E protein construct will induce the strongest ZIKV neutralizing antibodies. These include an E protein gene with either PrM or M gene or an E gene with honeybee melittin signal peptide (msp) in order to enhance the efficiency of glycosylation and intracellular processing of the E protein as shown previously with other viral proteins [27, 28]. In addition, we added the transmembrane domain and cytoplasmic tail of the VSV glycoprotein (Gtc) to produce VSV pseudovirion with the ZIKV E protein which may induce a strong immune response against the ZIKV E protein (FIG. 1).

[0128] We have employed two different serotypes of genetically modified avirulent VSV, Indiana serotype (VSV.sub.Ind-GML) and New Jersey serotype (VSV.sub.NJ-GMM) [22]. The priming vaccine vector should be antigenically distinct from the boosting vaccine vector in order to maximize boost effects. The VSV system provides an ideal prime-boost vaccine vectors since the antibody against the VSV.sub.Ind G protein does not neutralize VSV.sub.NJ and vice versa. We have inserted ZIKV PrM gene with E gene and E gene with msp and Gtc genes (FIG. 1). These rVSV.sub.Ind-GML-ZIKV E genes and rVSV.sub.NJ-GMM-ZIKV E genes have been recovered by the VSV reverse genetics system as previously described [22]. However, as described above, a highly efficient prime-boost vaccination can also be achieved with the same serotype of rVSV vectors (i.e., both the prime and boost are rVSV.sub.Ind or both the prime and boost are rVSV.sub.NJ).

[0129] 2. Intracellular and Secreted ZIKV E Proteins

[0130] The ZIKV E protein gene expression was determined in the cells infected with rVSV.sub.Ind-GML-ZIKV-E and rVSV.sub.NJ-GMM-ZIKV-E viruses. BHK21 cells were infected with multiplicity of infection (MOI) of 6 and harvested 6 hours post infection and lysed cells for detection of the intracellular E proteins. The infected cell lysates were electrophoresed and the presence of E protein was determined by Western blot analysis using an anti-ZIKV E antibody. FIG. 2A shows the presence of E protein in all four lanes. Lanes 1, 2 and 3 representing rVSV.sub.Ind-GML-ZIKV-PrM+E, rVSV.sub.Ind-GML-ZIKV-M+E, and rVSV.sub.Ind-GML-ZIKV-msp-E shows the same size E protein, but lane 4 representing rVSV.sub.Ind-GML-ZIKV-msp-E-Gtc shows a slightly larger than 55 kDa E protein. These results show that the E protein expression levels from three different rVSV-ZIKVs are about the same and also indicates that PrM, M and msp are cleaved off after the expression. The Gtc at the C-terminus of the E protein makes the protein slightly larger (FIG. 2A). We have determined the rVSV particle associated extracellular E proteins by pelleting the rVSV-ZIKV-E recombinant virus infected BHK21 cell supernatant (FIG. 2B). The rVSV-ZIKV infected cell supernatant was pelleted by ultracentrifugation through a 30% sucrose cushion. The pelleted samples were analyzed by SDS-PAGE followed by Western blot using the anti-ZIKV E antibody. Lanes 1, 2, and 3 showed varying amounts of E protein (FIG. 2B). Most secreted ZIKV E proteins are associated with rVSV as a part of pseudotype particles. It is not clear why there are two different sizes of E proteins associated with the rVSV pseudotype at this point.

[0131] The same experiments have been carried out using the rVSV.sub.NJ. GMM vector. We have constructed the same combinations of ZIKV E protein genes using rVSV.sub.NJ-GMM (FIG. 2C and FIG. 2D). The smaller E protein in the pelletable sample is less with VSV.sub.NJ-ZIKV-msp-E-Gtc.

[0132] The results shown in FIG. 2 suggest strongly that rVSV pseudotypes are formed with the ZIKV E protein. To determine whether or not ZIKV E proteins are associated with rVSV virions as a pseudovirion, we have conducted an immune-electron microscopy using gold particle conjugated secondary antibodies. A partially purified rVSV.sub.Ind-GML-ZIKV-msp-E-Gtc was incubated with either the anti-VSV G antibody or anti-ZIKV E antibody followed by a secondary anti-IgG conjugated with gold particles. FIG. 3A shows a detection of VSV G protein on the surface of rVSV.sub.Ind-GML virions. FIG. 3B shows a detection of ZIKV E protein on the surface of rVSV.sub.Ind-GML-ZIKV-msp-E-Gtc virions. FIG. 3C depicts a model for rVSV-ZIKV pseudovirion containing both VSV G protein and ZIKV E protein on the surface of the rVSV pseudovirions.

[0133] 3 Immune Responses Induced by rVSV-ZIKV-PrM+E and rVSV-ZIKV-Msp-E-Gtc

[0134] To determine optimal dose of rVSV-ZIKV vaccines, mice were immunized with eight different doses of rVSV-ZIKV-PrM+E, or rVSV-ZIKV-msp-E-Gtc. Mice were prime immunized with the rVSV.sub.Ind serotype and boost immunized with rVSV.sub.NJ serotype. Prime immunization with rVSV-ZIKV-PrM+E induced low level of ZIKV E specific antibodies at Day 13. Dosage of 5×10.sup.7 PFU/mouse or higher of rVSV-ZIKV-PrM+E induced slightly better ZIKV E specific antibody responses compare to the group of rVSV-mock, but it was not statistically significant (FIG. 4A). Priming immunization with rVSV.sub.Ind-ZIKV-msp-E-Gtc induced E specific antibodies slightly better than the rVSV.sub.Ind-ZIKV-prM+E in all dosage groups (FIG. 4B). After boosting immunization with rVSV.sub.NJ serotype, over all ZIKV E specific antibody was generated in a significantly increased level in all dosage groups of rVSV-ZIKV-prM+E and rVSV-ZIKV-msp-E-Gtc (FIGS. 4A and 4B). When the E specific antibody responses were compared between groups immunized with rVSV-ZIKV-msp-E-Gtc and rVSV-ZIKV-prM+E vaccine groups, rVSV-ZIKV-msp-E-Gtc induced higher level of E specific antibodies compare to the mice immunized with rVSV-ZIKV-prM+E in the highest dosage group (5×10.sup.8 PFU/mouse, FIG. 4C). Mice immunized with rVSV.sub.Ind-ZIKV-msp-E-Gtc induced equally strong or better immune responses in the highest dosage groups (5×10.sup.8 PFU/mouse) compare to mice immunized with a high dose of live ZIKV (FIG. 4C). Immune responses in mice immunized with a high dose of live ZIKV (2×10.sup.9 PFU) was strong as expected. The advantages for recombinant VSV-ZIKV vaccine are highly efficient immune responses, safety with the same protective immune responses, and the lower costs of vaccine production.

[0135] An effective vaccine should be able to induce strong neutralizing antibodies. For neutralization experiments, clinical isolate of ZIKV, ZIKV-Fortaleza/2015 (Brazil) [26], was pre-incubated with serially diluted antisera from immunized mice and residual infectious ZIKV was determined by plaque reduction neutralization assay on Vero cells and then the titer of neutralizing antibodies was determined. We found that immunization with both rVSV-ZIKV-prM+E and rVSV-ZIKV-msp-E-Gtc induced strong neutralizing antibodies. Prime-boost immunization with 5×10.sup.8 PFU of rVSV-ZIKV-msp-E-Gtc induced significantly higher neutralizing antibodies compared to prime-boost immunization of 2×10.sup.9 PFU of live ZIKV, the positive control group (FIG. 5). These immune response studies showed that rVSV.sub.Ind-ZIKV-msp-E-Gtc priming followed by VSV.sub.NJ-GMM-ZIKV-msp-E-Gtc boosting is a highly effective vaccination regimen to prevent ZIKV infection.

[0136] To further investigate immune responses of rVSV-ZIKV vaccine, we have tested the T cell immune responses against CD8 and CD4 specific peptides of ZIKV E protein by IFN-γ ELISpot assay. Splenocytes from both rVSV-ZIKV-PrM+E and rVSV-ZIKV-msp-E-Gtc immunized mice showed higher number of IFN-γ-secreting cells compare to mice immunized with live ZIKV, indicating that stronger T cell responses were induced by rVSV-ZIKV immunization. Interestingly, while rVSV-ZIKV-PrM+E immunized group showed higher T cell responses against CD8-specific peptide than rVSV-ZIKV-msp-E-Gtc immunized group, the rVSV-ZIKV-msp-E-Gtc immunized group showed similar or slightly higher T cell responses against CD4-specific peptide compare to the rVSV-ZIKV-PrM+E immunized group (FIG. 6). Because CD4+ T cells are well-known mediator for humoral immune responses, these results indicate that rVSV-ZIKV-msp-E-Gtc can induce stronger humoral responses, which is consistent with higher level of neutralizing antibody titers after rVSV-ZIKV-msp-E-Gtc immunization shown in FIG. 5.

[0137] 4. Protection Against Lethal ZIKV Challenge in Ifnar.sup.−/− Mice

[0138] The ultimate goal of any prophylactic vaccination is to protect the host upon challenging with lethal dose of the live virus. However, wild type mice are not suitable for ZIKV infection, since ZIKV-infected wild type mice do not show any evidence of morbidity or mortality. Thus, we have used the type 1 interferon receptor-deficient (B6(Cg)-Ifnar 1.sup.tm1.2Ees/J, Ifnar.sup.−/−) mice as an animal model for ZIKV challenge studies, since this Ifnar.sup.−/− mouse model has been used for studies of pathogenesis of wide range of viruses [29, 30, 31], especially ZIKV [32, 33, 34]. Ifnar.sup.−/− mice were immunized with 5×10.sup.8 PFU of rVSV.sub.Ind-ZIKV-PrM+E or rVSV.sub.Ind-ZIKV-msp-E-Gtc followed with boosting immunization with 5×10.sup.8 PFU of rVSV.sub.NJ-ZIKV-PrM+E or rVSV.sub.NJ-ZIKV-msp-E-Gtc in two-week intervals. In addition, we used rVSV.sub.Ind-Mock and rVSV.sub.NJ-Mock without the ZIKV gene insert as negative controls. We observed that all rVSV vectors with or without the ZIKV gene insert did not show any adverse effects to Ifnar.sup.−/− mice prior to the ZIKV challenge. These results reconfirm that both serotypes of our rVSV vectors are safe to use as vaccine vectors.

[0139] Two weeks after boosting immunization, 1×10.sup.3 PFU of ZIKV-Fortaleza/2015 (Brazil) was challenged to immunized Ifnar.sup.−/− mice via intraperitoneal route, and their survival rate and loss of body weight were observed for 14 days post the virus challenge (PC). All rVSV-ZIKV-PrM+E and rVSV-ZIKV-msp-E-Gtc immunized mice survived after the ZIKV-Fortaleza/2015 (Brazil) challenge, while all rVSV-Mock vector-immunized negative control mice died within 8 days PC (FIG. 7A). In addition, loss of body weight was not observed in all rVSV-ZIKV-PrM+E and rVSV-ZIKV-msp-E-Gtc immunized mice, whereas rVSV-Mock vector immunized mice showed drastic loss of body weight (FIG. 7B). These results show clearly that both the rVSV-ZIKV-PrM+E vaccine and rVSV-ZIKV-msp-E-Gtc vaccine induced protective immune responses. Furthermore, the challenge studies clearly demonstrated that the modified E protein alone without PrM can induce protective immune responses against ZIKV infection.

TABLE-US-00001 TABLE 1 Primers used for cloning SEQ ID Primers NO Sequences PrME-F 18 5′-TCCCCGCGGGTTTAAACGATGAACGATATGAA AAAAACTAACAGAATTCAAAATGGGCGCAGATACT AGTGTCGGA-3′ PrME-R 19 5′-TCCCCGCGGACGCGTTTAAGCAGAG ACAGCT GTGGAT-3′ ME-F 20 5′-TCCCCGCGGGTTTAAACGATGAACGATATGAA AAAAACTAACAGAATTCAAAATGGTGGAACCAGAT GACGTCGATT-3′ E-F 21 5′-TCCCCGCGGGTTTAAACGATGAACGATATGAA AAAAACTAAC-3′ Emsp-mega 22 5′-GCTGACTCCTATGCACCTGATCGCATAGATGT AAGAAATGTACAC-3′ EGct-mega 23 5′ATTTTTGGAGCAGCTTTCAAATCAAGCTCTATT GCCTCTTTTTTCTTT-3′ EGct-R 24 5′-TCCCC GCGGACGCGTTTACTTCCCAAGTCGG TTCATC-3′

TABLE-US-00002 NJ-M (F); (SEQ ID NO: 25) 5′-tccccgcggttaattaagatgaacgatatgaaaaaaact-3′  NJ-M(M48R + M51R); (SEQ ID NO: 26) 5′- tatcatataaatctcgatcctctcgtccgaagaagtca-3′  NJ-G (R); (SEQ ID NO: 27) 5′-tccccgcggttaattaatttagcggaagtgagccat-3′  Ind(GLM) M (F); SEQ ID NO: 28) 5′-cgggcggccgcttaattaaactatgaaaaaaagtaacagat -3′  Ind (GLM) M(M51R); (SEQ ID NO: 29) 5′-catgagtgtctcgctcgtcaac-3′  Ind (GLM) M(L111A); (SEQ ID NO: 30) 5′-aagatcttggcttttgcaggttcttc-3′  Ind(GLM) M (R); (SEQ ID NO: 31) 5′-cgggcggccgctagactagctcatttg-3′ 

TABLE-US-00003 TABLE 2 Neucleotide Sequence Comparison between M Genes of VSV Indiana serotype, Wild Type (SEQ ID NO: 1) and a Mutant G21E/L111A/M51R (SEQ ID NO 2) 1                                                   50 SEQ ID NO: 1: ATGAGTTCCT TAAAGAAGAT TCTCGGTCTG AAGGGGAAAG GTAAGAAATC SEQ ID NO: 2: ATGAGTTCCT TAAAGAAGAT TCTCGGTCTG AAGGGGAAAG GTAAGAAATC 51                                                   100 SEQ ID NO: 1: TAAGAAATTA GGGATCGCAC CACCCCCTTA TGAAGAGGAC ACTAACATGG SEQ ID NO: 2: TAAGAAATTA GAAATCGCAC CACCCCCTTA TGAAGAGGAC ACTAACATGG 101                                                  150 SEQ ID NO: 1: AGTATGCTCC GAGCGCTCCA ATTGACAAAT CCTATTTTGG AGTTGACGAG SEQ ID NO: 2: AGTATGCTCC GAGCGCTCCA ATTGACAAAT CCTATTTTGG AGTTGACGAG 151                                                  200 SEQ ID NO: 1: ATGGACACTC ATGATCCGCA TCAATTAAGA TATGAGAAAT TCTTCTTTAC SEQ ID NO: 2: CGAGACACTC ATGATCCGCA TCAATTAAGA TATGAGAAAT TCTTCTTTAC 201                                                  250 SEQ ID NO: 1: AGTGAAAATG ACGGTTAGAT CTAATCGTCC GTTCAGAACA TACTCAGATG SEQ ID NO: 2: AGTGAAAATG ACGGTTAGAT CTAATCGTCC GTTCAGAACA TACTCAGATG 251                                                  300 SEQ ID NO: 1: TGGCAGCCGC TGTATCCCAT TGGGATCACA TGTACATCGG AATGGCAGGG SEQ ID NO: 2: TGGCAGCCGC TGTATCCCAT TGGGATCACA TGTACATCGG AATGGCAGGG 301                                                  350 SEQ ID NO: 1: AAACGTCCCT TCTACAAGAT CTTGGCTTTT TTGGGTTCTT CTAATCTAAA SEQ ID NO: 2: AAACGTCCCT TCTACAAGAT CTTGGCTTTT GCAGGTTCTT CTAATCTAAA 351                                                  400 SEQ ID NO: 1: GGCCACTCCA GCGGTATTGG CAGATCAAGG TCAACCAGAG TATCACGCTC SEQ ID NO: 2: GGCCACTCCA GCGGTATTGG CAGATCAAGG TCAACCAGAG TATCACGCTC 401                                                  450 SEQ ID NO: 1: ACTGTGAAGG CAGGGCTTAT TTGCCACACA GAATGGGGAA GACCCCTCCC SEQ ID NO: 2: ACTGTGAAGG CAGGGCTTAT TTGCCACACA GAATGGGGAA GACCCCTCCC 451                                                  500 SEQ ID NO: 1: ATGCTCAATG TACCAGAGCA CTTCAGAAGA CCATTCAATA TAGGTCTTTA SEQ ID NO: 2: ATGCTCAATG TACCAGAGCA CTTCAGAAGA CCATTCAATA TAGGTCTTTA 501                                                  550 SEQ ID NO: 1: CAAGGGAACG GTTGAGCTCA CAATGACCAT CTACGATGAT GAGTCACTGG SEQ ID NO: 2: CAAGGGAACG GTTGAGCTCA CAATGACCAT CTACGATGAT GAGTCACTGG 551                                                  600 SEQ ID NO: 1: AAGCAGCTCC TATGATCTGG GATCATTTCA ATTCTTCCAA ATTTTCTGAT SEQ ID NO: 2: AAGCAGCTCC TATGATCTGG GATCATTTCA ATTCTTCCAA ATTTTCTGAT 601                                                  650 SEQ ID NO: 1: TTCAGAGATA AGGCCTTAAT GTTTGGCCTG ATTGTCGAGA AAAAGGCATC SEQ ID NO: 2: TTCAGAGATA AGGCCTTAAT GTTTGGCCTG ATTGTCGAGA AAAAGGCATC 651                                                  700 SEQ ID NO: 1: TGGAGCTTGG GTCCTGGATT CTGTCAGCCA CTTCAAATGA SEQ ID NO: 2: TGGAGCTTGG GTCCTGGATT CTGTCAGCCA CTTCAAATGA

TABLE-US-00004 TABLE 3 Amino Acid Sequence Comparison between M Proteins of VSV Indiana serotype Wild Type (SEQ ID NO: 3) and a Mutant G21E/L111A/M151R (SEQ ID NO: 4) 1                    21                             50 SEQ ID NO: 3: MSSLKKILGL KGKGKKSKKL GIAPPPYEED TNMEYAPSAP IDKSYFGVDE SEQ ID NO: 4: MSSLKKILGL KGKGKKSKKL EIAPPPYEED TNMEYAPSAP IDKSYFGVDE 51                                                  100 SEQ ID NO: 3: MDTHDPHQLR YEKFFFTVKM TVRSNRPFRT YSDVAAAVSH WDHMYIGMAG SEQ ID NO: 4: RDTHDPHQLR YEKFFFTVKM TVRSNRPFRT YSDVAAAVSH WDHMYIGMAG 101       111                                       150 SEQ ID NO: 3: KRPFYKILAF LGSSNLKATP AVLADQGQPE YHAHCEGRAY LPHRMGKTPP SEQ ID NO: 4: KRPFYKILAF AGSSNLKATP AVLADQGQPE YHAHCEGRAY LPHRMGKTPP 151                                                  200 SEQ ID NO: 3: MLNVPEHFRR PFNIGLYKGT VELTMTIYDD ESLEAAPMIW DHFNSSKFSD SEQ ID NO: 4: MLNVPEHFRR PFNIGLYKGT VELTMTIYDD ESLEAAPMIW DHFNSSKFSD 201                           229                    250 SEQ ID NO: 3: FRDKALMFGL IVEKKASGAW VLDSVSHFK SEQ ID NO: 4: FRDKALMFGL IVEKKASGAW VLDSVSHFK

TABLE-US-00005 TABLE 4 Nucleotide Sequence Comparison between M Genes of VSV New Jersey serotype Wild Type (SEQ ID NO: 5) and Mutants, G22E/M48R/M51R (SEQ ID NO: 6) and G22E/L110A/M48R/M51R (SEQ ID NO: 7) 1                                                   50 SEQ ID NO: 5: ATGAGTTCCT TCAAAAAGAT TCTGGGATTT TCTTCAAAAA GTCACAAGAA SEQ ID NO: 6: ATGAGTTCCT TCAAAAAGAT TCTGGGATTT TCTTCAAAAA GTCACAAGAA ID NO: 7: ATGAGTTCCT TCAAAAAGAT TCTGGGATTT TCTTCAAAAA GTCACAAGAA 51                                                  100 SEQ ID NO: 5: ATCAAAGAAA CTAGGCTTGC CACCTCCTTA TGAGGAATCA AGTCCTATGG SEQ ID NO: 6: ATCAAAGAAA CTAGAATTGC CACCTCCTTA TGAGGAATCA AGTCCTATGG SEQ ID NO: 7: ATCAAAGAAA CTAGAATTGC CACCTCCTTA TGAGGAATCA AGTCCTATGG 101                                                 150 SEQ ID NO: 5: AGATTCAACC ATCTGCCCCA TTATCAAATG ACTTCTTCGG AATGGAGGAT SEQ ID NO: 6: AGATTCAACC ATCTGCCCCA TTATCAAATG ACTTCTTCGG ACGAGAGGAT SEQ ID NO: 7: AGATTCAACC ATCTGCCCCA TTATCAAATG ACTTCTTCGG ACGAGAGGAT 151                                                  200 SEQ ID NO: 5: ATGGATTTAT ATGATAAGGA CTCCTTGAGA TATGAGAAGT TCCGCTTTAT SEQ ID NO: 6: CGAGATTTAT ATGATAAGGA CTCCTTGAGA TATGAGAAGT TCCGCTTTAT SEQ ID NO: 7: CGAGATTTAT ATGATAAGGA CTCCTTGAGA TATGAGAAGT TCCGCTTTAT 201                                                  250 SEQ ID NO: 5: GTTGAAGATG ACTGTTAGAG CTAACAAGCC CTTCAGATCG TATGATGATG SEQ ID NO: 6: GTTGAAGATG ACTGTTAGAG CTAACAAGCC CTTCAGATCG TATGATGATG SEQ ID NO: 7: GTTGAAGATG ACTGTTAGAG CTAACAAGCC CTTCAGATCG TATGATGATG 251                                                  300 SEQ ID NO: 5: TCACCGCAGC GGTATCACAA TGGGATAATT CATACATTGG AATGGTTGGA SEQ ID NO: 6: TCACCGCAGC GGTATCACAA TGGGATAATT CATACATTGG AATGGTTGGA SEQ ID NO: 7: TCACCGCAGC GGTATCACAA TGGGATAATT CATACATTGG AATGGTTGGA 301                                                  350 SEQ ID NO: 5: AAGCGTCCTT TCTACAAGAT AATTGCTCTG ATTGGCTCCA GTCATCTGCA SEQ ID NO: 6: AAGCGTCCTT TCTACAAGAT AATTGCTCTG ATTGGCTCCA GTCATCTGCA SEQ ID NO: 7: AAGCGTCCTT TCTACAAGAT AATTGCTGCA ATTGGCTCCA GTCATCTGCA 351                                                  400 SEQ ID NO: 5: AGCAACTCCA GCTGTGTTGG CAGACTTAAA TCAACCAGAG TATTATGCCA SEQ ID NO: 6: AGCAACTCCA GCTGTGTTGG CAGACTTAAA TCAACCAGAG TATTATGCCA SEQ ID NO: 7: AGCAACTCCA GCTGTGTTGG CAGACTTAAA TCAACCAGAG TATTATGCCA 401                                                  450 SEQ ID NO: 5: CACTAACAGG TCGTTGTTTT CTTCCTCACC GACTCGGATT GATCCCACCG SEQ ID NO: 6: CACTAACAGG TCGTTGTTTT CTTCCTCACC GACTCGGATT GATCCCACCG SEQ ID NO: 7: CACTAACAGG TCGTTGTTTT CTTCCTCACC GACTCGGATT GATCCCACCG 451                                                  500 SEQ ID NO: 5: ATGTTTAATG TGTCCGAAAC TTTCAGAAAA CCATTCAATA TTGGGATATA SEQ ID NO: 6: ATGTTTAATG TGTCCGAAAC TTTCAGAAAA CCATTCAATA TTGGGATATA SEQ ID NO: 7: ATGTTTAATG TGTCCGAAAC TTTCAGAAAA CCATTCAATA TTGGGATATA 501                                                  550 SEQ ID NO: 5: CAAAGGGACT CTCGACTTCA CCTTTACAGT TTCAGATGAT GAGTCTAATG SEQ ID NO: 6: CAAAGGGACT CTCGACTTCA CCTTTACAGT TTCAGATGAT GAGTCTAATG SEQ ID NO: 7: CAAAGGGACT CTCGACTTCA CCTTTACAGT TTCAGATGAT GAGTCTAATG 551                                                  600 SEQ ID NO: 5: AAAAAGTCCC TCATGTTTGG GAATACATGA ACCCAAAATA TCAATCTCAG SEQ ID NO: 6: AAAAAGTCCC TCATGTTTGG GAATACATGA ACCCAAAATA TCAATCTCAG SEQ ID NO: 7: AAAAAGTCCC TCATGTTTGG GAATACATGA ACCCAAAATA TCAATCTCAG 601                                                  650 SEQ ID NO: 5: ATCCAAAAAG AAGGGCTTAA ATTCGGATTG ATTTTAAGCA AGAAAGCAAC SEQ ID NO: 6: ATCCAAAAAG AAGGGCTTAA ATTCGGATTG ATTTTAAGCA AGAAAGCAAC SEQ ID NO: 7: ATCCAAAAAG AAGGGCTTAA ATTCGGATTG ATTTTAAGCA AGAAAGCAAC 651                                                 700 SEQ ID NO: 5: GGGAACTTGG GTGTTAGACC AATTGAGTCC GTTTAA SEQ ID NO: 6: GGGAACTTGG GTGTTAGACC AATTGAGTCC GTTTAA SEQ ID NO: 7: GGGAACTTGG GTGTTAGACC AATTGAGTCC GTTTAA

TABLE-US-00006 TABLE 5 Amino Acid Sequence Comparison between M Proteins of VSV New Jersey serotype Wild Type (SEQ ID NO: 8) and Mutants, G22E/M48R/M51R (SEQ ID NO: 9) and G22E/L110A/M48R/M51R (SEQ ID NO: 10) 1                      22                         48 50 SEQ ID NO: 8: MSSFKKILGF SSKSHKKSKK LGLPPPYEES SPMEIQPSAP LSNDFFGMED SEQ ID NO: 9: MSSFKKILGF SSKSHKKSKK LELPPPYEES SPMEIQPSAP LSNDFFGRED SEQ ID NO: 10: MSSFKKILGF SSKSHKKSKK LELPPPYEES SPMEIQPSAP LSNDFFGRED 51                                                  100 SEQ ID NO: 8: MDLYDKDSLR YEKFRFMLKM TVRANKPFRS YDDVTAAVSQ WDNSYIGMVG SEQ ID NO: 9: RDLYDKDSLR YEKFRFMLKM TVRANKPFRS YDDVTAAVSQ WDNSYIGMVG SEQ ID NO: 10: RDLYDKDSLR YEKFRFMLKM TVRANKPFRS YDDVTAAVSQ WDNSYIGMVG 101     110                                         150 SEQ ID NO: 8: KRPFYKIIAL IGSSHLQATP AVLADLNQPE YYATLTGRCF LPHRLGLIPP SEQ ID NO: 9: KRPFYKIIAL IGSSHLQATP AVLADLNQPE YYATLTGRCF LPHRLGLIPP SEQ ID NO: 10: KRPFYKIIAA IGSSHLQATP AVLADLNQPE YYATLTGRCF LPHRLGLIPP 151                                                  200 SEQ ID NO: 8: MFNVSETFRK PFNIGIYKGT LDFTFTVSDD ESNEKVPHVW EYMNPKYQSQ SEQ ID NO: 9: MFNVSETFRK PFNIGIYKGT LDFTFTVSDD ESNEKVPHVW EYMNPKYQSQ SEQ ID NO: 10: MFNVSETFRK PFNIGIYKGT LDFTFTVSDD ESNEKVPHVW EYMNPKYQSQ 201                                                  250 SEQ ID NO: 8: IQKEGLKFGL ILSKKATGTW VLDQLSPFK SEQ ID NO: 9: IQKEGLKFGL ILSKKATGTW VLDQLSPFK SEQ ID NO: 10: IQKEGLKFGL ILSKKATGTW VLDQLSPFK

[0140] Zika Virus Genes Inserted into VSV Vector:

TABLE-US-00007 Msp-E-Gtc (SEQ ID NO: 11) atgaaattcttagtcaacgttgcccttgtttttatggtcgtgtacatttc ttacatctatgcgatcaggtgcataggagtcagcaatagggactttgtgg aaggtatgtcaggtgggacttgggttgatgttgtcttggaacatggaggt tgtgtcaccgtaatggcacaggacaaaccgactgtcgacatagagctggt tacaacaacagtcagcaacatggcggaggtaagatcctactgctatgagg catcaatatcggacatggcttcggacagccgctgcccaacacaaggtgaa gcctaccttgacaagcaatcagacactcaatatgtctgcaaaagaacgtt agtggacagaggctggggaaatggatgtggactttttggcaaagggagcc tggtgacatgcgctaagtttgcatgctccaagaaaatgaccgggaagagc atccagccagagaatctggagtaccggataatgctgtcagttcatggctc ccagcacagtgggatgatcgttaatgacacaggacatgaaactgatgaga atagagcgaaggttgagataacgcccaattcaccaagagccgaagccacc ctggggggttttggaagcctaggacttgattgtgaaccgaggacaggcct tgacttttcagatttgtattacttgactatgaataacaagcactggttgg ttcacaaggagtggttccacgacattccattaccttggcacgctggggca gacaccggaactccacactggaacaacaaagaagcactggtagagttcaa ggacgcacatgccaaaaggcaaactgtcgtggttctagggagtcaagaag gagcagttcacacggcccttgctggagctctggaggctgagatggatggt gcaaagggaaggctgtectctggccacttgaaatgtcgcctgaaaatgga taaacttagattgaagggcgtgtcatactccttgtgtaccgcagcgttca cattcaccaagatcccggctgaaacactgcacgggacagtcacagtggag gtacagtacgcagggacagatggaccttgcaaggttccagctcagatggc ggtggacatgcaaactctgaccccagttgggaggttgataaccgctaacc ccgtaatcactgaaagcactgagaactctaagatgatgctggaacttgat ccaccatttggggactcttacattgtcataggagtcggggagaagaagat cacccaccactggcacaggagtggcagcaccattggaaaagcatttgaag ccactgtgagaggtgccaagagaatggcagtcttgggagacacagcctgg gactttggatcagttggaggcgctctcaactcattgggcaagggcatcca tcaaatttttggagcagctttcaaatcaagctctattgcctcttttttct ttatcatagggttaatcattggactattcttggttctccgagttggtatt tatctttgcattaaattaaagcacaccaagaaaagacagatttatacaga catagagatgaaccgacttgggaagtaa PreME (SEQ ID NO: 12) atgggcgcagatactagtgtcggaattgttggcctcctgctgaccacagc tatggcagcggaggtcactagacgtgggagtgcatactatatgtacttgg acagaaacgacgctggggaggccatatcttttccaaccacattggggatg aataagtgttatatacagatcatggatcttggacacatgtgtgatgccac catgagctatgaatgccctatgctggatgagggggtggaaccagatgacg tcgattgttggtgcaacacgacgtcaacttgggttgtgtacggaacctgc catcacaaaaaaggtgaagcacggagatctagaagagctgtgacgctccc ctcccattccactaggaagctgcaaacgcggtcgcaaacctggttggaat caagagaatacacaaagcacttgattagagtcgaaaattggatattcagg aaccctggcttcgcgttagcagcagctgccatcgcttggcttttgggaag ctcaacgagccaaaaagtcatatacttggtcatgatactgctgattgccc cggcatacagcatcaggtgcataggagtcagcaatagggactttgtggaa ggtatgtcaggtgggacttgggttgatgttgtcttggaacatggaggttg tgtcaccgtaatggcacaggacaaaccgactgtcgacatagagctggtta caacaacagtcagcaacatggcggaggtaagatcctactgctatgaggca tcaatatcggacatggcttcggacagccgctgcccaacacaaggtgaagc ctaccttgacaagcaatcagacactcaatatgtctgcaaaagaacgttag tggacagaggctggggaaatggatgtggactttttggcaaagggagcctg gtgacatgcgctaagtttgcatgctccaagaaaatgaccgggaagagcat ccagccagagaatctggagtaccggataatgctgtcagttcatggctccc agcacagtgggatgatcgttaatgacacaggacatgaaactgatgagaat agagcgaaggttgagataacgcccaattcaccaagagccgaagccaccct ggggggttttggaagcctaggacttgattgtgaaccgaggacaggccttg acttttcagatttgtattacttgactatgaataacaagcactggttggtt cacaaggagtggttccacgacattccattaccttggcacgctggggcaga caccggaactccacactggaacaacaaagaagcactggtagagttcaagg acgcacatgccaaaaggcaaactgtcgtggttctagggagtcaagaagga gcagttcacacggcccttgctggagctctggaggctgagatggatggtgc aaagggaaggctgtcctctggccacttgaaatgtcgcctgaaaatggata aacttagattgaagggcgtgtcatactccttgtgtaccgcagcgttcaca ttcaccaagatcccggctgaaacactgcacgggacagtcacagtggaggt acagtacgcagggacagatggaccttgcaaggttccagctcagatggcgg tggacatgcaaactctgaccccagttgggaggttgataaccgctaacccc gtaatcactgaaagcactgagaactctaagatgatgctggaacttgatcc accatttggggactcttacattgtcataggagtcggggagaagaagatca cccaccactggcacaggagtggcagcaccattggaaaagcatttgaagcc actgtgagaggtgccaagagaatggcagtcttgggagacacagcctggga ctttggatcagttggaggcgctctcaactcattgggcaagggcatccatc aaatttttggagcagctttcaaatcattgtttggaggaatgtcctggttc tcacaaattctcattggaacgttgctgatgtggttgggtctgaacacaaa gaatggatctatttcccttatgtgcttggccttagggggagtgttgatct tcttatccacagctgtctctgcttaa

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[0180] It should be understood that although the present invention has been specifically disclosed by certain aspects, embodiments, and optional features, modification, improvement and variation of such aspects, embodiments, and optional features can be resorted to by those skilled in the art, and that such modifications, improvements and variations are considered to be within the scope of this disclosure.

[0181] The invention has been described broadly and generically herein. Each of the narrower species and subgeneric groupings falling within the generic disclosure also form part of the invention. In addition, where features or aspects of the invention are described in terms of Markush groups, those skilled in the art will recognize that the invention is also thereby described in terms of any individual member or subgroup of members of the Markush group.