Calibration verification for optical particle analyzers
11181455 · 2021-11-23
Assignee
Inventors
Cpc classification
International classification
Abstract
Provided are particle analyzers and related methods for verifying calibration status of the particle analyzer. The method includes the steps of providing an optical particle analyzer and modulating a power applied to a source of EMR. The method includes the steps of, in response to the modulating step, inducing a detector signal waveform and analyzing the detector signal waveform to determine a value of at least one diagnostic parameter associated with one or more of the source of EMR, an optical assembly, a chamber, a detector, and an optical collection system of the optical particle analyzer. The method includes the step of determining a calibration status of the optical particle analyzer based on the one or more determined values of the at least one diagnostic parameter.
Claims
1. A method for determining calibration status of an optical particle analyzer comprising: providing an optical particle analyzer including: a source of electromagnetic radiation (EMR) for generating a beam of said EMR; a chamber for containing a sample medium and for receiving said beam of EMR; an optical assembly in optical communication with the source of EMR for directing the beam of EMR from to the chamber; a detector for detecting scattered radiation from said beam of EMR; an optical collection system for directing said scattered radiation from the beam of EMR from the chamber and to the detector; modulating a power applied to the source of EMR; in response to the modulating step, inducing a detector signal waveform; analyzing the detector signal waveform to determine a value of at least one diagnostic parameter associated with one or more of the source of EMR, the optical assembly, the chamber, the detector, and the optical collection system; and determining the calibration status of the optical particle analyzer based on the one or more determined values of the at least one diagnostic parameter; wherein the including step includes: first receiving, by the detector, the scattered radiation form said beam of EMR at a first radiant power level corresponding to a first power level applied to the source of EMR, and second receiving, by the detector, the scattered radiation from said beam of EMR at a second radiant power level corresponding to a second power level applied to the source of EMR; and wherein the detector signal waveform has: a leading edge defined by a leading edge function; a first signal amplitude; and a second signal amplitude.
2. The method of claim 1, wherein the source of EMR comprises at least one of a laser, a diode laser, a strip diode laser, a light emitting diode, and an incandescent lamp.
3. The method of claim 1, wherein the chamber comprises a flow chamber.
4. The method of claim 3, wherein the sample medium is a fluid, said method further comprising flowing the fluid through the flow chamber during the modulating step.
5. The method of claim 4, wherein the flowing step comprises filtering the fluid upstream of the flow chamber.
6. The method of claim 1, wherein the sample medium includes particles.
7. The method of claim 1, wherein the modulating step further comprises switching the power applied to the source of EMR from a first power level to a second power level, wherein the switching step comprises switching the power applied to the source of EMR according to a switching waveform having a frequency, a duty cycle, a first switching amplitude corresponding to the first power level, and a second switching amplitude corresponding to the second power level.
8. The method of claim 1, further comprising maintaining the power applied to the source of EMR at a second power level for a time selected over the range of 1 nanoseconds to 10 milliseconds.
9. The method of claim 1, wherein the optical particle analyzer further includes amplification circuitry operably connected to the detector for amplifying a detector signal in response to the inducing step, and wherein the second receiving step includes changing an energization state of the amplification circuitry from a first state to a second state corresponding to the first and second radiant power levels, respectively.
10. The method of claim 9, wherein the leading edge of the detector signal waveform corresponds to the change of the energization state of the amplification circuitry from the first state to the second state.
11. The method of claim 9, wherein the at least one diagnostic parameter is further associated with the amplification circuitry.
12. The method of claim 1, wherein the at least one diagnostic parameter includes a detector signal waveform peak amplitude defined as a value of a difference between a value of the first signal amplitude and a value of the second signal amplitude value, and wherein the analyzing step further comprises determining: the value of the first signal amplitude; the value of the second signal amplitude; and the value of the difference between the first and second amplitude values; wherein the optical particle analyzer further includes amplification circuitry operably connected to the detector for amplifying a detector signal in response to the inducing step, and wherein the detector signal waveform peak amplitude is associated with an operational condition of the source of EMR, the chamber, the optical assembly, the optical collection system, the detector, and the amplification circuitry.
13. The method of claim 1, wherein the at least one diagnostic parameter includes an elapsed time for the leading edge, and wherein the analyzing step includes determining the elapsed time for the leading edge; and wherein the optical particle analyzer further includes drive circuitry for the source of EMR, and wherein the elapsed time for the leading edge is associated with an operational condition of the drive circuitry.
14. The method of claim 1, wherein the at least one diagnostic parameter includes the leading edge function, and wherein the analyzing step includes determining the leading edge function of the leading edge; and wherein the leading edge function is associated with an operational condition of the source of EMR, the optical assembly, the chamber, the detector, and the optical collection system of the optical particle analyzer.
15. The method of claim 1, wherein the determining step further comprises comparing the one or more determined values of the at least one diagnostic parameter with corresponding values of at least one of the respective calibration parameter determined at a prior calibration of the optical particle analyzer.
16. The method of claim 15, wherein the comparing step includes determining a difference between the one or more determined values of the at least one diagnostic parameter and the corresponding values of each respective calibration parameter determined at the prior calibration, and determining the calibration status of the optical particle analyzer based on the determined difference.
17. An optical particle analyzer comprising: a source of electromagnetic radiation (EMR) for generating a beam of said EMR; a chamber for containing a sample medium and for receiving said beam of EMR; an optical assembly in optical communication with the source for directing the beam of EMR from the source of EMR to the chamber; a detector for detecting scattered radiation from said beam of EMR; an optical collection system for directing scattered radiation from the beam of EMR from the chamber and to the detector; and a processor operably connected to the source and the detector, wherein the processor is programmed to: modulate a power applied to the source of EMR from a first power level to a second power level; analyze a scattered radiation detector signal waveform induced by the modulation of the power applied to the source of EMR; determine a value of at least one diagnostic parameter associated with one or more of the source of EMR, the chamber, the optical assembly, the detector, and the optical collection system; and determine a calibration status of the optical particle analyzer based on the one or more determined values of the at least one diagnostic parameter: wherein the source of EMR is a laser for generating a laser beam; said chamber is a flow-chamber: wherein the at least one diagnostic parameter includes an amplitude of a peak of the scattered radiation detector signal waveform corresponding to a difference in detector signal amplitudes between; the scattered radiation detected by the detector from the laser beam at a first radiant power level from the laser having the applied power at the first power level; and the scattered radiation detected by the detector from the laser beam at a second radiant power level from the laser having the applied power at the second power level; wherein, in response to the scattered radiation detector signal waveform induced by the modulation of the power applied to the source of EMR, the processor is further programmed to; first receive a first detector signal from the detector, the first detector signal representative of scattered radiation from said beam of EMR at a first radiant power level corresponding to the first power level applied to the source of EMR; and second receive a second detector signal from the detector, the second detector signal representative of scattered radiation from said bean of EMR at a second radiant power level corresponding to the second power level applied to the source of EMR; and wherein the scattered radiation detector signal waveform has: a leading edge defined by leading edge function; a first signal amplitude; and a second signal amplitude.
18. The optical particle analyzer of claim 17, wherein the sample medium is a fluid, and wherein the optical particle analyzer further comprises a flow system for flowing the fluid through the flow chamber.
19. The optical particle analyzer of claim 18, further comprising a filter for filtering the fluid upstream of the flow chamber.
20. The optical particle analyzer of claim 17, wherein the sample medium includes particles.
21. The optical particle analyzer of claim 17, wherein, to modulate the power applied to the source of EMR, the processor is further programmed to switch the power applied to the source of EMR from the first power level to the second power level according to a switching waveform having a frequency, a duty cycle, a first switching amplitude corresponding to the first power level, and a second switching amplitude corresponding to the second power level.
22. The optical particle analyzer of claim 17, wherein the processor is further programmed to maintain the power applied to the source of EMR at a second power level for a time selected over the range of 1 nanoseconds to 10 milliseconds.
23. The optical particle analyzer of claim 17, further comprising amplification circuitry operably connected to the detector for amplifying the first and second detector signals prior to receipt by the processor, and wherein, in response to the modulation of the power applied to the source of EMR, an energization state of the amplification circuitry changes from a first state to a second state corresponding to the first and second radiant power levels, respectively.
24. The optical particle analyzer of claim 23, wherein the leading edge of the scattered radiation detector signal waveform corresponds to the change of the energization state of the amplification circuitry from the first state to the second state.
25. The optical particle analyzer of claim 23, wherein the at least one diagnostic parameter is further associated with the amplification circuitry.
26. The optical particle analyzer of claim 17, wherein the at least one diagnostic parameter includes a scattered radiation detector signal waveform peak amplitude defined as a value of a difference between a value of the first signal amplitude and a value of the second signal amplitude value, and wherein, to analyze the scattered radiation detector signal waveform induced by the modulation of the power applied to the source of EMR, the processor is further programmed to determine: the value of the first signal amplitude; the value of the second signal amplitude; and the value of the difference between the first and second amplitude values; the optical particle analyzer further comprising amplification circuitry operably connected to the detector for amplifying the first and second detector signals prior to receipt by the processor, wherein the scattered radiation detector signal waveform peak amplitude is associated with an operational condition of the source of EMR, the chamber, the optical assembly, the optical collection system, the detector, and the amplification circuitry.
27. The optical particle analyzer of claim 17, wherein the at least one diagnostic parameter includes an elapsed time for the leading edge, and wherein, to analyze the scattered radiation detector signal waveform induced by the modulation of the power applied to the source of EMR, the processor is further programmed to determine the elapsed time for the leading edge; further comprising drive circuitry for the source of EMR, wherein the elapsed time for the leading edge is associated with an operational condition of the drive circuitry.
28. The optical particle analyzer of claim 17, wherein the at least one diagnostic parameter includes the leading edge function, and wherein, to analyze the scattered radiation detector signal waveform induced by the modulation of the power applied to the source of EMR, the processor is further programmed to determine the leading edge function of the leading edge; wherein the leading edge function is associated with an operational condition of the source of EMR, the optical assembly, the chamber, the detector, and the optical collection system of the optical particle analyzer.
29. The optical particle analyzer of claim 17, wherein, to determine the value of at least one diagnostic parameter, the processor is further programmed to compare the one or more determined values of the at least one diagnostic parameter with corresponding values of at least one of the respective calibration parameter determined at a prior calibration of the optical particle analyzer.
30. The optical particle analyzer of claim 29, wherein, to compare the one or more determined values of the at least one diagnostic parameter with the corresponding values of at least one of the respective calibration parameter determined at the prior calibration of the optical particle analyzer, the processor is further programmed to determine a difference between the one or more determined values of the at least one diagnostic parameter and the corresponding values of each respective calibration parameter determined at the prior calibration; and the processor is further programmed to determine the calibration status of the optical particle analyzer based on the determined difference.
Description
BRIEF DESCRIPTION OF THE DRAWINGS
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DETAILED DESCRIPTION OF THE INVENTION
(21) In the following description, numerous specific details of the devices, device components and methods of the present invention are set forth in order to provide a thorough explanation of the precise nature of the invention. It will be apparent, however, to those of skill in the art that the invention can be practiced without these specific details.
(22) In general, the terms and phrases used herein have their art-recognized meaning, which can be found by reference to standard texts, journal references and contexts known to those skilled in the art. The following definitions are provided to clarify their specific use in the context of the invention.
(23) The term “sample medium” refers to the matter or collection of matter comprising a sample to be measured by an optical particle analyzer. As an example, an optical particle analyzer may sample air from an environment. The air may or may not contain particles and it is thus one object of using the optical particle analyzer to determine whether or not the air in the environment has particles. In this example, the sample medium is the air of the environment.
(24) The term “diagnostic parameter” refers to a measurable quantity or quality determined analytically through one or more outputs of the optical particle analyzer such as a detector signal waveform. The values of diagnostic parameters are determined at times between successful calibration events of the optical particle analyzer.
(25) The term “diagnostic parameter associated with” refers to one or more values of the determined diagnostic parameters being representative of operational conditions and functioning of particle component(s) in the optical particle analyzer. For instance, values of a particular diagnostic parameter may vary according to a radiant power of scattered light incident upon on a photodiode-based detector of the optical particle analyzer. In this case, a determined value for such a diagnostic parameter that is less than an expected value (e.g., an out of specification result) may, for example and without limitation, indicate an operational problem with the source of electromagnetic radiation (e.g., a laser) whose beam is scattered and subsequently detected by the detector of the optical particle analyzer.
(26) The term “operational condition” refers to a state of functioning of a particular component or set of components of the optical particle analyzer. Operational condition may be a strictly binary status where the component(s) are either functional or are non-functional. Operational condition may be a status on a continuum of functionality ranging from fully functional to fully non-functional. Such a continuum of functionality may include intermediate state(s) such as nearing an out of specification status and/or requiring some maintenance operation according to a predetermined schedule. As described in detail herein, values of diagnostic parameters determined using disclosed systems and methods may be associated with the operational condition of the respective component(s) of the optical particle analyzer for which the diagnostic parameters are associated.
(27) The term “calibration status” refers to a particular functional state of the optical particle analyzer reflecting whether the optical particle analyzer is “in-calibration” or “out-of-calibration.” The calibration status of a particular optical particle analyzer is the status since the last successful calibration was performed and/or certified for that same particular particle analyzer. Calibration status may be a binary status where the particular optical particle analyzer is either in-calibration or is out-of-calibration. Calibration status may be a status on a continuum of statuses ranging from in-calibration to out-of-calibration. Such a continuum may include an intermediate status such as nearing an out-of-calibration status, nearing a time for a scheduled calibration, and/or approaching or exceeding one or more predetermined control alert limit(s). Depending on the application of interest, an analyzer may be defined as “calibrated” if it is within a user-defined tolerance, such as providing a parameter that is within 10%, within 5%, within 1% or within 0.1% of absolute calibrated.
(28) The term “calibration parameter” takes on the same meaning as “diagnostic parameter,” with the exception that values of calibration parameters are determined during the same time for which successful calibration events are performed for the optical particle analyzer (e.g., determined between the start of the calibration event and the end of the calibration event).
(29) The term “leading edge function” refers to an equation that defines or at least approximates the leading edge of the detector signal waveform.
(30) The term “energization state” refers to a qualitative and/or quantitative measure of the stored electrical energy in a component of the optical particle analyzer.
(31) The term “operably connected to” refers to two or more functionally-related components being coupled to one another for purposes of flow of electric current and/or flow of data signals. This coupling of the two or more components may be a wired connection and/or a wireless connection. The two or more components that are so coupled via the wired and/or wireless connection may be proximate one another (e.g., in the same room or in the same housing as the optical particle analyzer) or they may be separated by some distance in physical space (e.g., in a different building from the location of the optical particle analyzer).
(32) The term “optical communication” refers to components that are arranged in a manner that allows light or electromagnetic radiation to transfer between the components.
(33) The term “radiation” refers to energy in the form of waves and/or particles, such as energy undergoing emission or transmission through space or through a material medium. Preferably for some methods and applications, the term “radiation” refers to electromagnetic radiation. The term “electromagnetic radiation” and “light” are used synonymously in the present description and refer to waves, and/or photons, of electric and magnetic fields. As used herein, electromagnetic radiation includes, but is not limited to, radio waves, microwaves, infrared, visible light, ultraviolet, X-rays, and gamma rays. Electromagnetic radiation useful for the methods of the present invention includes, but is not limited to ultraviolet light, visible light, infrared light, or any combination of these having wavelengths between approximately 100 nanometers (nm) to approximately 15 microns (μm). The term “scattered radiation” refers to radiation resulting from scattering of radiation, such as radiation resulting from scattering of at least a portion of a beam (or, energy content thereof) of electromagnetic radiation. For example, an interaction between a beam of electromagnetic radiation, such as a laser beam, and matter, such as particles present in a medium through which the beam is being transmitted, may comprise scattering of at least a portion of the beam, or energy content thereof, of the electromagnetic radiation. For example, at least a fraction of photons of a beam of electromagnetic radiation may be scattered due interaction with matter or a non-uniformity in a medium. The terms “scattering” and “scatter” refer to a process by which radiation, such as electromagnetic radiation, is forced to deviate from a straight trajectory due to interaction with one or more non-uniformities in a medium through which the radiation is being emitted or transmitted. For example, scattering may refer to waves, or their quanta, photons, of electromagnetic radiation undergoing deviation(s) from one straight trajectory to at least a second trajectory due to interaction with matter or non-uniformity, such as one or more particles, in a medium, such as a fluid (e.g., gas, air, liquid, etc.), through which the waves, or photons, of electromagnetic radiation are being emitted or transmitted.
(34) The terms “approximately” and “about” are used interchangeably and refer to a value that is within 20%, within 10%, within 5%, or, optionally, equal to a given reference value. For example, a wavelength that is about 100 nm is any wavelength that is within 20%, within 10%, within 5%, or preferably in some applications equivalent to 100 nm.
(35) “Particles” refers to small objects which are often regarded as contaminants. A particle can be any material created by the act of friction, for example, when two surfaces come into mechanical contact and there is mechanical movement. Particles can be composed of aggregates of material, such as dust, dirt, smoke, ash, water, soot, metal, minerals, or any combination of these or other materials or contaminants. “Particles” may also refer to biological particles, for example, viruses, spores and microorganisms including bacteria, fungi, archaea, protists, other single cell microorganisms and specifically those microorganisms having a size on the order of 1-15 μm. A particle may refer to any small object which absorbs or scatters light and is thus detectable by an optical particle counter. As used herein, “particle” is intended to be exclusive of the individual atoms or molecules of a carrier fluid, for example water molecules, process chemical molecules, oxygen molecules, helium atoms, nitrogen molecules, etc. Some embodiments of the present invention are capable of detecting, sizing, and/or counting particles comprising aggregates of material having a size greater than 10 nm, 20 nm, 30 nm, 50 nm, 100 nm, 500 nm, 1 μm or greater, or 10 μm or greater. Specific particles include particles having a size selected from 20 nm to 50 nm, 50 nm to 50 μm, a size selected from 100 nm to 10 μm, or a size selected from 500 nm to 5 μm.
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(37) The optical particle analyzer 100 shown in
(38) The optical particle analyzer 100 shown in
(39) The optical particle analyzer 100 shown in
(40) The optical particle analyzer 100 shown in
(41) The optical particle analyzer 100 shown in
(42) In an embodiment, processor(s) 124 may execute software stored in memory device(s) 126 for controlling various components of the optical particle analyzer 100 during operation. In an embodiment, the processor(s) 124 may control the source of EMR 102 via the drive circuitry 136 (e.g., by controlling user 130-specified frequencies, voltages, currents, waveforms, duty cycles, and other control parameters implemented by the drive circuitry 136). In an embodiment, the processor(s) 124 may be operably coupled to the flow system 109 (e.g., flow controller, pump, valves, conduits, etc.) and the processor(s) 124 may control the flow system 109 by, for example, controlling user 130-specified flow rates and other control parameters implemented by the flow system 109. In an embodiment, the processor(s) 124 may receive the detector signal 134 and, after decoding information encoded by the detector signals 134, store the decoded information in the memory device(s) 126. The optical particle analyzer 100 may include a power supply 142 for providing electric power to the various components and systems of the optical particle analyzer 100 requiring electric power to function.
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(44) The method 200 shown in
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(46) In an embodiment, the processor(s) 124 of the optical particle analyzer 100 is/are programmed to modulate the power applied to the source of EMR 102 from the first power level to the second power level. In embodiments for which the source of EMR 102 includes the laser, the processor(s) 124 is/are programmed to modulate the power applied to the laser from the first power level to the second power level. In an embodiment, to modulate the power applied to the source of EMR 102, the processor(s) 124 is/are programmed to switch the power applied to the source of EMR 102 from the first power level to the second power level. In embodiments for which the source of EMR 102 includes the laser, the processor(s) 124 is/are programmed to switch the power applied to the laser from the first power level to the second power level. In an embodiment, values of the first and second power levels are defined by the user 130 of the optical particle analyzer 100 prior to or concurrently with the performance of the modulating step 204. In an embodiment, the values of the first and second power levels are stored in memory device(s) 126 in a power settings block 402 of the data structure 400. In an embodiment, the processor(s) 124 read the data encoding the values for the first and second power levels from the power settings block 402 prior to or concurrently with executing the program instructions stored in the modulating module 304 for the modulating step 204.
(47) In an embodiment, the modulating step 204 of the method 200 shown in
(48) In an embodiment, the switching step may be performed as a part of a computer-implemented method by processor(s) 124 executing program instructions stored as software 302 in the memory device(s) 126. In an embodiment, the processor(s) 124 perform the switching step by executing the program instructions encoded in a switching module 305 of the software 302.
(49) In an embodiment, to switch the power applied to the source of EMR 102 or the laser, the processor(s) 124 is/are programmed to switch a flow of electric current from a power supply (e.g., power supply 142, shown in
(50) In an embodiment, the modulating step 204 of the method 200 includes a maintaining step. The maintaining step may include maintaining the power applied to the source of EMR 102 at the second power level for a time selected over the range of 1 nanosecond (ns) to 10 milliseconds (ms). In an embodiment, the maintaining step includes maintaining the power applied to the source of EMR 102 at the second power level for a time selected over the range of 10 ms to 1 second (s). In embodiments for which the source of EMR 102 includes the laser, the maintaining step includes maintaining the power applied to the laser at the second power level for a time selected over the range of 1 ns to 10 ms. In an embodiment, the maintaining step includes maintaining the power applied to the laser at the second power level for a time selected over the range of 10 ms to 1 s.
(51) In an embodiment, the maintaining step may be performed as a part of a computer-implemented method by processor(s) 124 executing program instructions stored as software 302 in the memory device(s) 126. In an embodiment, the processor(s) 124 perform the maintaining step by executing the program instructions encoded in a maintaining module 306 of the software 302.
(52) In an embodiment, to maintain the power applied to the source of EMR 102 at the second power level for a time selected over the range of 1 nanoseconds (ns) to 10 milliseconds (ms) or for a time selected over the range of 10 ms to 1 seconds (s), the processor(s) 124 is/are programmed to maintain a flow of electric current from the power supply 142 to the drive circuitry 136. In an embodiment, a value for the time to maintain the power applied to the source of EMR 102 at the second power level is defined by the user 130 of the optical particle analyzer 100 prior to or concurrently with the performance of the modulating step 204. In an embodiment, the value for the time to maintain the power applied to the source of EMR 102 at the second power level is stored in a time settings block 406 of the data structure 400. In an embodiment, the processor(s) 124 read the data encoding the value for the time to maintain the power applied to the source of EMR 102 at the second power level from the time settings block 406 prior to or concurrently with executing the program instructions stored in the maintaining module 306 for the maintaining step.
(53) In embodiments for which the chamber 106 includes the flow chamber, the method 200 may include a flowing step. In an embodiment, the flowing step includes flowing the sample medium 108 including the fluid through the flow chamber. In an embodiment, the flowing step includes flowing the sample medium 108 including the fluid and including particles through the flow chamber. In an embodiment, the flowing step includes a filtering step. In an embodiment, the filtering step includes filtering the sample medium 108 including the fluid. In an embodiment, the filtering step includes filtering the sample medium 108 including the fluid upstream of the flow chamber. In an embodiment, the flowing step includes flowing the sample medium 108 including the fluid through the flow chamber during the modulating step 204. In an embodiment, the method 200 is performed in the absence of filtering the sample medium. In an embodiment, the method 200 is performed in the absence of flowing the sample medium. In an embodiment, the method 200 is performed in the absence of flowing the sample medium and in the absence of filtering the sample medium.
(54) In an embodiment, the flowing step may be performed as a part of a computer-implemented method by processor(s) 124 executing program instructions stored as software 302 in the memory device(s) 126. In an embodiment, the processor(s) 124 perform the flowing step by executing the program instructions encoded in a flowing module 307 of the software 302.
(55) In an embodiment, to flow the sample medium 108 including the fluid through the flow chamber, the processor(s) 124 is/are programmed to control a flow rate of the flow system 109. In an embodiment, a value for the flow rate is defined by the user 130 of the optical particle analyzer 100 prior to or concurrently with the performance of the modulating step 204. In an embodiment, the value for the flow rate for the flow system 109 is stored in the flow rate settings block 408 of the data structure 400. In an embodiment, the processor(s) 124 read the data encoding the value for the flow rate from the flow rate settings block 408 prior to or concurrently with executing the program instructions stored in the flowing module 307 for the flowing step.
(56) The method 200 shown in
(57) In an embodiment, the inducing step 206 may include a step of second receiving, by the detector 118, the scattered radiation 119 from the beam of EMR 104. In an embodiment, the second receiving step may be performed in method 200 after the first receiving step. In an embodiment, the second receiving step may include receiving, by the detector 118, the scattered radiation 119 from the beam of EMR 104 at a second radiant power level corresponding to the second power level applied to the source of EMR 102. In embodiments for which the source of EMR 102 includes the laser, the second receiving step may include receiving, by the detector 118, the scattered radiation 119 from the laser beam at the second radiant power level corresponding to the second power level applied to the laser.
(58) In an embodiment, the first and/or second receiving steps may be performed as a part of a computer-implemented method by processor(s) 124 executing program instructions stored as software 302 in the memory device(s) 126. In an embodiment, the processor(s) 124 perform the first receiving step by executing the program instructions encoded in a first receiving module 308 of the software 302.
(59) In an embodiment, concurrently with or after the detector 118 first receives the scattered radiation 119 from the source of EMR 102 or the laser at the first radiant power level, the resultant detector signal 134 undergoes analog-to-digital conversion either before or after the detector signal 134 passes through the amplification circuitry 132. In an embodiment, the processor(s) 124 is/are programmed to receive the detector signal 134 converted to digital form. In an embodiment, the processor(s) 124 is/are programmed to decode the detector signal 134. In an embodiment, the processor(s) 124 is/are programmed to store the information encoded by the detector signal 134 as data in a detector signal block 410 of the data structure 400. In an embodiment, the processor(s) 124 is/are programmed to transmit the data in the detector signal block 410 to the display 150 to, for example, graphically render the detector signal waveform 502 in a human readable form. In an embodiment, the processor(s) 124 is/are programmed to store the detector signal waveform 502 as data in a signal waveform block 412 of the data structure 400.
(60) In an embodiment, the processor(s) 124 perform the second receiving step by executing the program instructions encoded in a second receiving module 310 of the software 302. In an embodiment, concurrently with or after the detector 118 second receives the scattered radiation 119 from the source of EMR 102 or the laser at the second radiant power level, the resultant detector signal 134 undergoes analog-to-digital conversion either before or after the detector signal 134 passes through the amplification circuitry 132. In an embodiment, the detector 118 is configured to perform the first and second receiving steps by sampling the scattered radiation 119 from the source of EMR 102 or the laser beam at the first and second radiant power levels, respectively. In an embodiment, the processor(s) 124 is/are programmed to control the sampling of the detector 118 at a sampling frequency. In an embodiment, a value for the sampling frequency is defined by the user 130 of the optical particle analyzer 100 prior to or concurrently with the performance of the modulating step 204. In an embodiment, the value for the sampling frequency for the detector 118 is stored in a detector settings block 414 of the data structure 400. In an embodiment, the processor(s) 124 read the data encoding the value for the sampling frequency from the detector settings block 414 prior to or concurrently with executing the program instructions stored in the modulating module 304 for the modulating step 204.
(61) In an embodiment, the second receiving step may include a changing step. In an embodiment, the changing step may include changing an energization state of the amplification circuitry 132 from a first state (e.g., a first energization state whereby the amplification circuitry 132 contains a first level of stored electrical energy) to a second state (e.g., a second energization state whereby the amplification circuitry 132 contains a second level of stored electrical energy). In an embodiment, the first level of stored energy for the first state is less than the second level of stored energy for the second state. In an embodiment, the first level of stored energy for the first state is greater than the second level of stored energy for the second state. In an embodiment, the first state corresponds to the first radiant power level for the scattered radiation 119 from the source of EMR 102. In embodiments for which the source of EMR 102 includes the laser, the first state corresponds to the first radiant power level for the scattered radiation 119 from the laser. In an embodiment, the second state corresponds to the second radiant power level for the scattered radiation 119 from the source of EMR 102. In embodiments for which the source of EMR 102 includes the laser, the second state corresponds to the second radiant power level for the scattered radiation 119 from the laser.
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(63) In an embodiment, the leading edge 504 of the detector signal waveform 502 corresponds to the change of the energization state of the amplification circuitry 132 from the first state to the second state. For the example detector signal waveform 502 plot 500 shown in
(64) In an embodiment, the detector signal waveform 502 includes a peak 510. For the example detector signal waveform 502 plot 500 shown in
(65) The method 200 shown in
(66) In an embodiment, the analyzing step 208 includes determining a value of the first signal amplitude 506 of the detector signal waveform 502. In an embodiment, the analyzing step 208 includes determining a value of the second signal amplitude 508 of the detector signal waveform 502. In an embodiment, the analyzing step 208 includes determining a value of a difference between the values of the first 506 and the second 508 signal amplitudes of the detector signal waveform 502.
(67) In an embodiment, the analyzing step 208 includes determining the value of the amplitude 511 of the peak 510 of the detector signal waveform 502. For the example detector signal waveform 502 plot 500 shown in
(68) In an embodiment, the value of the amplitude of the peak 510 of the detector signal waveform 502 determined in the analyzing step 208 is associated with (e.g., and is indicative of) an operational condition of the source of EMR 102, the chamber 106, the optical assembly 112, the optical collection system 120, the detector 118, and the amplification circuitry 132 of the optical particle analyzer 100. In embodiments for which the source of EMR 102 includes the laser, the value of the amplitude of the peak 510 of the detector signal waveform 502 determined in the analyzing step 208 is associated with an operational condition of the laser and the operational condition of the chamber 106, the optical assembly 112, the optical collection system 120, the detector 118, and the amplification circuitry 132. In embodiments for which the source of EMR 102 includes the laser and the chamber 106 includes the flow chamber, the value of the amplitude of the peak 510 of the detector signal waveform 502 determined in the analyzing step 208 is associated with an operational condition of the flow chamber, the operational condition of the laser, and the operational condition of the optical assembly 112, the optical collection system 120, the detector 118, and the amplification circuitry 132. In an embodiment, a value of the amplitude of the peak 510 determined in the analyzing step 208 that is equal to a peak 510 amplitude value previously determined and stored at or about the time of the last calibration indicates: A healthy source of EMR 102 A healthy chamber 106 A healthy optical assembly 112 A healthy optical collection system 120 A healthy detector 118 Healthy amplification circuitry 132
(69) In an embodiment, a value of the amplitude of the peak 510 determined in the analyzing step 208 that is less than a peak 510 amplitude value previously determined and stored at or about the time of the last calibration indicates at least one of: A possibly unstable or failed source of EMR 102 A possibly contaminated, misaligned, or failed optical assembly 112 A possibly contaminated or failed optical collection system 120 A possibly contaminated or failed detector 118 Possibly failed amplification circuitry 132
(70) In an embodiment, a value of the amplitude of the peak 510 determined in the analyzing step 208 that is greater than a peak 510 amplitude value previously determined and stored at or about the time of the last calibration indicates at least one of: A possibly misaligned optical assembly 112 Possibly failed amplification circuitry 132
(71) In an embodiment, the at least one diagnostic parameter includes an elapsed time (e.g., elapsed time 512 shown in
(72) In an embodiment, the leading edge function is associated with an operational condition of the source of EMR 102, the optical assembly 112, the chamber 106, the detector 118, and the optical collection system 120 of the optical particle analyzer 100. In embodiments for which the source of EMR 102 includes the laser, the leading edge function determined in the analyzing step 208 is associated with an operational condition of the laser and the operational condition of the chamber 106, the optical assembly 112, the optical collection system 120, the detector 118, and the amplification circuitry 132. In embodiments for which the source of EMR 102 includes the laser and the chamber 106 includes the flow chamber, the leading edge function determined in the analyzing step 208 is associated with an operational condition of the flow chamber, the operational condition of the laser, and the operational condition of the optical assembly 112, the optical collection system 120, the detector 118, and the amplification circuitry 132.
(73) In an embodiment, the analyzing step 208 may be performed as a part of a computer-implemented method by processor(s) 124 executing program instructions stored as software 302 in the memory device(s) 126. In an embodiment, the processor(s) 124 perform the analyzing step 208 by executing the program instructions encoded in an analyzing module 314 of the software 302.
(74) In an embodiment, for the analyzing step 208, the processor(s) 124 is/are programmed to analyze the scattered radiation detector signal waveform 502 induced by the modulation of the power applied to the source of EMR 102 or the laser. In an embodiment, to analyze the scattered radiation detector signal waveform 502 induced by the modulation of the power applied to the source of EMR 102 or the laser, the processor(s) 124 is/are programmed to determine: (a) the value of the first signal amplitude 506; (b) the value of the second signal amplitude 508; and (c) the value of the difference between the first 506 and second 508 amplitude values of the detector signal waveform 502. In an embodiment, to analyze the scattered radiation detector signal waveform 502 induced by the modulation of the power applied to the source of EMR 102 or the laser, the processor(s) 124 is/are programmed to read the detector signal waveform 502 data stored in the signal waveform block 412 of the data structure 400. In an embodiment, the processor(s) 124 is/are programmed to store the determined values of the first 506 and second 508 amplitude values of the detector signal waveform 502 and the determined value of the difference between the first 506 and second 508 amplitude values in a waveform analysis block 416 of the data structure 400.
(75) In an embodiment, to analyze the scattered radiation detector signal waveform 502 induced by the modulation of the power applied to the source of EMR 102 or the laser, the processor(s) 124 is/are programmed to determine the elapsed time 512 for the leading edge 504 of the detector signal waveform 502. In an embodiment, to analyze the scattered radiation detector signal waveform 502 induced by the modulation of the power applied to the source of EMR 102 or the laser, the processor(s) 124 is/are programmed to determine the leading edge function of the leading edge 504 of the detector signal waveform 502. In an embodiment, to determine the leading edge function of the leading edge 504, the processor(s) 124 utilize one or more curve fitting algorithms including, for example and without limitation, an exponential curve fitting algorithm. In an embodiment, the processor(s) 124 is/are programmed to store the determined value of the elapsed time 512 and the determined leading edge function for the leading edge 504 of the detector signal waveform 502 in the waveform analysis block 416 of the data structure 400.
(76) In an embodiment, the processor(s) 124 is/are programmed to determine leading edge function parameters of the leading edge function. For example, for a linear curve fitting algorithm (e.g., y=m*x+b) for the leading edge function, the processor(s) 124 may determine the values of the function parameters m and b. For example, for an exponential growth curve fitting algorithm (e.g., y=P*e.sup.rx) for the leading edge function, the processor(s) 124 may determine the values of the function parameters P and r. In an embodiment, the processor(s) 124 is/are programmed to store the determined values of the leading edge function parameters in the waveform analysis block 416 of the data structure 400.
(77) In an embodiment, the processor(s) 124 is/are programmed to determine an operational condition of one or more of the component(s) of the optical particle analyzer (e.g., those component(s) shown and described above for the optical particle analyzer 100). In an embodiment, the processor(s) 124 determine the operational condition of the component(s) of the optical particle analyzer 100 based on the determined values of the diagnostic parameters. In an embodiment, determining, by the processor(s) 124, the operational condition of the component(s) of the optical particle analyzer 100 may include comparing the determined value(s) of the diagnostic parameter(s) with one or more specification value(s) for the respective optical particle analyzer 100 component(s). In an embodiment, user 130-predetermined values for specification value(s) for the respective optical particle analyzer 100 component(s) are stored in a specifications block 417 of the data structure 400. In an embodiment, results of the operational conditions of the component(s) of the optical particle analyzer 100 are stored, by the processor(s) 124, in an operational status block 418 of the data structure 400.
(78) The method 200 shown in
(79) In an embodiment, the determining step 210 may be performed as a part of a computer-implemented method by processor(s) 124 executing program instructions stored as software 302 in the memory device(s) 126. In an embodiment, the processor(s) 124 perform the determining step 210 by executing the program instructions encoded in a determining module 316 of the software 302. In an embodiment, the processor(s) 124 is/are programmed to determine the value of the at least one diagnostic parameter based on the analysis of the detector signal waveform 502 in the analysis step 208. In an embodiment, for determining the value of the at least one diagnostic parameter, the processor(s) 124 is/are programmed to read one or more of: the first 506 and second 508 amplitude values, the value of the difference between the first 506 and second 508 amplitude values, the elapsed time 512 value, the leading edge function, and the leading edge function parameters, from the waveform analysis block 416 of the data structure 400. In an embodiment, after performing the determining step 210, the processor(s) 124 is/are programmed to store the determined value(s) of the at least one diagnostic parameter in a diagnostic parameter block 419 of the data structure 400.
(80)
(81) In an embodiment, the determining step 210 includes determining the calibration status of the optical particle analyzer 100 based on the value (e.g., determined in the comparing step and/or block 601) of the difference between the one or more determined values of the at least one diagnostic parameter and the corresponding values of each respective calibration parameter determined at the prior calibration. In an embodiment, the processor(s) 124 may perform a first query 602. In the first query 602, the processor(s) 124 compare the difference value determined in block 601 with a specification value stored in the specifications block 417 of the data structure 400. In an embodiment, the specification value stored in the specifications block 417 is user-defined or is manufacturer-defined prior to or concurrently with the performance of the determining step 210. In an embodiment, if the difference value determined in block 601 is greater than the specification value stored in the specifications block 417, then the determined calibration status of the optical particle analyzer 100 is out of calibration. In an embodiment, the processor(s) 124 may store the determined calibration status in a calibration status block 428 of the data structure 400. In an embodiment, the processor(s) 124 may alert the user 130 that the optical particle analyzer 100 is out of calibration. Upon determining that the calibration status of the optical particle analyzer 100 is out of calibration, the processor(s) 124 proceed to an end state of the state diagram 600.
(82) In an embodiment, if the difference value determined in block 601 is not greater than the specification value, then the processor(s) 124 may perform a second query 604. In the second query 604, the processor(s) 124 compare the difference value determined in block 601 with an alert value stored in a control alerts block 426 of the data structure 400. In an embodiment, the alert value stored in the control alerts block 426 is defined by the user 130 of the optical particle analyzer 100 prior to or concurrently with the performance of the determining step 210. In an embodiment, if the difference value determined in block 601 is greater than the alert value stored in the control alerts block 426, then the determined calibration status of the optical particle analyzer 100 is in calibration, but with a notify status regarding an approaching out-of-calibration status. In an embodiment, the processor(s) 124 may store the determined calibration status in the calibration status block 428 of the data structure 400. In an embodiment, the processor(s) 124 may notify the user 130 that the optical particle analyzer 100 has a determined diagnostic parameter difference value that exceeds an alert value.
(83) In an embodiment, upon determining that the calibration status of the optical particle analyzer 100 is in calibration (including in the case where the determined diagnostic parameter difference value does not exceed the alert value), the processor(s) 124 may perform a third query 606. In the third query 606, the processor(s) 124 determine whether or not values of other types of diagnostic parameters (e.g., for peak 510 amplitude where, for the first iteration of state diagram 600, the diagnostic parameter was elapsed time 512) are available to be read from the diagnostic parameters block 419 of the data structure 400. In an embodiment, if the processor(s) 124 determine that there are values of other types of diagnostic parameters available to be read from the diagnostic parameters block 419 of the data structure 400, the processor(s) 124 commence executing a second iteration of the state diagram 600. Otherwise, the processor(s) 124 proceed to the end state of the state diagram 600.
(84) The invention can be further understood by the following non-limiting examples.
EXAMPLE 1
(85) Check Pulses Effectiveness Investigation
(86) An IsoAir®-310P instrument equipped with the laser as the source of EMR 102 (30 mW laser power) was used as a test instrument to perform the following experiments in Example 1 to determine what percentage of post-Bessel filter laser-on pulse is derived from light collection mirrors and what percentage is derived from a source other than the mirrors.
(87)
(88)
(89)
(90)
(91) Check-Pulses Effectiveness Investigation Summary: As shown by the plots of
EXAMPLE 2
(92) Study of Allowed LASAIR-IV Signal Loss to Maintain Passing as-Found Calibration Results
(93) Example 2 was a study of a manufacturing cell calibration program data-base typical first channel size resolution results calculated for each instrument model type (Particle Measuring Systems, Inc.). Minimum ISO-21501-4 allowed size error was determined from sizing and counting efficiency as-found test limits. Gaussian distribution sizing distribution size error was then converted to amplitude error by applying the LASAIR-III theoretical sizing calculations. The final result was calculated to represent the maximum amount of particle signal loss that could be observed while the instrument would theoretically pass the as-found calibration requirements of ISO-21501-4.
(94) Summary table of Example 2 study results:
(95) TABLE-US-00001 First 30% 30% CH # of 10% C.E. C.E. First Size Units Size Size Size Amplitude Inst CH Res in Error Error Error Error Model Size Study Study Limit Limit Limit Limit 310C 0.3 um 7.4% 1477 30 nm 12.1 nm 4.0% 20.2% 310B 0.3 um 7.6% 141 30 nm 12.4 nm 4.1% 20.7% 350L o.3 um 5.0% 534 30 nm 8.1 nm 2.7% 13.6% 5100 0.5 um 15.0 1057 50 nm 42.9 nm 8.6% 17.7% 510 (bio) 0.5 um 10.0% 1 50 nm 27.5 nm 5.5% 11.4%
(96) LASAIR-IV Allowed Signal Loss Summary: The Check Pulses test would require the ability to detect an 11.4% decrease in light level, in order to ensure passing as-found calibration verification results. The effect of possible 60 Hz ambient light noise may be minimized by averaging multiple pulses. This may improve the accuracy of this test.
(97) The results of Example 2 demonstrate that the laser-on pulse may be detected in greater than or equal to 2 ms. The entire decay (e.g., represented in
EXAMPLE 3
(98) Description of Detector Board Low Speed, Check Pulses, Peak and Hold ADC Circuit
(99)
(100) Stage 1 of circuit 1900: Voltage gain amplifier with bandwidth limiting (Q=4.0). Allows gain scaling and low pass filtering of check pulses signal. Signal originated from the high gain Bessel filter output. Primary components: (1 stage) AD8034 FET op amp, +/−12Vdc PS.
(101) Stages 2 & 3 of circuit 1900: Classical closed-loop peak-and-hold circuit, with reset feature. Primary components: (2 stages) AD8034 FET op amp, (1) PMEG3002AEB Schottky Barrier diode, hold charge capacitor, isolation resistor to buffer first amp output from charge capacitor, ADG1402 and ADG1401 CMOS SPST peak-and-hold reset switches, SN65LVDS2DBVR differential line receiver to drive reset switches from main PCB, isolation resistor to buffer switch from charge capacitor, +/−12Vdc PS.
(102) Stage 4 of circuit 1900: Voltage gain amplifier with bandwidth limiting (Q=0.25). Voltage divider on input to scale peak-and-hold+12Vdc maximum output to the ADC128S022 ADC 3.3Vdc maximum input range. Low pass filtering at output. Primary components: (1 stage) AD8034 FET op amp, +/−12Vdc PS.
(103) Due to the charge capacitor circuit Schottky barrier diode voltage drop, peak and hold circuit signal loss will be less than 100 mV in circuit 1900. Accounting for this signal loss in the firmware calculations may be accounted for in order to accurately calculate signal loss percentage.
(104) The various components costs of the Detector Board Low Speed, Check Pulses, Peak and Hold ADC Circuit (e.g., circuit 1900) (e.g., components ADG1401, ADG1402, AD8034, PMEG3005AEA, ADC128S022, SN65LVDS2DBVR) is low, totaling less than about $15.00.
EXAMPLE 4
(105) Timing Considerations for Low Speed, Check Pulses, Peak and Hold ADC Circuit
(106)
(107)
(108) The results of Example 4 demonstrate the ability of the laser power-up check pulses to be reliably operated at a cycle frequency of approximately 4 Hz. This enables the system to run several laser power-up check pulses measurements at instrument power-up, or on customer demand, thereby allowing the instrument to offer a quick and convenient automated particle counting health diagnostic feature.
(109) Peak and Hold ADC Timing Considerations Summary:
(110) TABLE-US-00002 Event Description Time #1 Sample flow off or filtered unknown #2 Turn laser off 1 ms #3 Allow 250 ms for AC coupling circuit settling 250 ms #4 Reset peak and hold circuit 1 ms #5 Turn laser on 1 ms #6 Allow time for laser-on pulse to peak 2 ms #7 Measure laser-on pulse peak value 1 ms Total Total single check pulses cycle time 256 ms
(111) Steps (i.e., referred to as “events” in the table, above) 1-7 may be repeated as needed to average out 60 Hz ambient light noise. The hold value may be held for >10 ms with less than 1% voltage sag. Accuracy may be improved by ensuring there are no particles present in the laser beam when performing this test. This may be accomplished while sampling filtered air. In many cases, it may be possible to perform the disclosed method with unfiltered ambient air as the sample medium 108, with no flow through sample block (e.g., chamber 106). With use of unfiltered ambient air, detecting outlier data point(s) caused by particle(s) in the beam may be performed when averaging multiple laser-on pulses. The peak and hold circuit may be frequency tuned to diminish the circuit's response to particle activity while sampling normal instrument flow.
(112) The results of Example 4 demonstrate that the check-pulses cycle event time may be greater than or equal to 256 mms. As such, up to 4 samples may be averaged in a 1 second time period.
EXAMPLE 5
(113) Ambient Air Particle Effect on Low Speed, Check Pulses, Peak and Hold ADC Circuit
(114)
(115)
(116) The peak and hold circuit are designed to not have sufficient speed to react to particle events that happen to occur during the check pulses laser power ramp-up function. This allows the check pulses function to be fairly immune to the presence of moderate particulate levels present during the check pulses function.
(117) In
(118)
(119)
(120)
(121)
(122)
(123) The five check pulses, peak and hold circuit, responding to a check pulses, laser power-up function, with particles present in the laser beam, are 3.3875Vdc, 3.375Vdc, 3.4125Vdc, 3.375Vdc, and 3.3875Vdc. The three-sigma spread of these five measurements is a mean value of 3.3875Vdc, with a+/−0.04593 Vdc (1.36%), three-sigma spread. This data indicates that there is 95% probability of measuring a check pulses function with particle contamination present, to measure with 1.8% of the no particles present check pulses function. Therefore, the check pulses function is relatively immune to the presence of moderate particulate contamination in the air the instrument is operating in.
(124) The results of Example 5 shown in
(125) Option #1: Ensure that only filtered air is in the sample block when the check-pulses test is performed. The instrument blower would then be running filtered air through the sample block during the test. This might best be accomplished as a customer activated diagnostic health check, which requests the customer to place a zero count filter on the instrument. In this case, the most accurate check-pulses results would be obtained. Tuning the frequency response of the peak and hold circuit to largely not respond to faster particle and pulse noise event times may minimize the influence of stray particles and pulse noise. Ensuring there is filtered air may also improve accuracy for performing the signal/noise test.
(126) Option #2: No filtered instrument inlet required. If the check-pulses test ensures the instrument blower is off, initial testing indicates a decreased probability of a particle being present in the beam during the test. In the experiments, approximately >10% of the check-pulses test runs had a particle present in the laser beam. However, some particle migration through the laser beam was observed even when the blower was off. These slow moving particle events tended to be longer in duration than the laser-on pulse, and therefore may have been recognizable as particle events that could be excluded in the check-pulses averaging. The less accurate check-pulses test under these conditions may be implemented as a boot-up diagnostic test with more wide-open pass criteria than option #1.
(127) Option #3: No filtered instrument inlet required. The effect of ambient air particles and pulse noise events may be minimized by intentionally tuning the frequency response of the peak and hold circuit to largely not respond to faster particle event times. The time domain distance between the laser power-up slow speed check pulses pulse (e.g., 2 ms rise-time, 125 Hz), and the higher frequency particle pulses (e.g., 2 μs rise-time, 125 Khz), is a factor of one-thousand. This allows the check pulses circuit to be designed with a low-pass time function that allows the circuit to be fairly immune to the presence of moderate levels of particle contamination in the sampled air present during the check pulses measurement function.
(128) The required response time for detecting the laser-on pulse is about 2 ms signal leading edge (e.g., 2 ms elapsed time 512 of the leading edge 504), while particle and pulse noise events may have leading edge rise times of about 2 μs. In this case, to ensure fast transit times for all particles, the instrument blower may be on during the check-pulse test. The less accurate check-pulses test under these conditions may be implemented as a boot-up diagnostic test with more wide-open pass criteria than option #1.
(129) All references throughout this application, for example patent documents including issued or granted patents or equivalents; patent application publications; and non-patent literature documents or other source material; are hereby incorporated by reference herein in their entireties, as though individually incorporated by reference, to the extent each reference is at least partially not inconsistent with the disclosure in this application (for example, a reference that is partially inconsistent is incorporated by reference except for the partially inconsistent portion of the reference).
(130) The terms and expressions which have been employed herein are used as terms of description and not of limitation, and there is no intention in the use of such terms and expressions of excluding any equivalents of the features shown and described or portions thereof, but it is recognized that various modifications are possible within the scope of the invention claimed. Thus, it should be understood that although the present invention has been specifically disclosed by preferred embodiments, exemplary embodiments and optional features, modification and variation of the concepts herein disclosed may be resorted to by those skilled in the art, and that such modifications and variations are considered to be within the scope of this invention as defined by the appended claims. The specific embodiments provided herein are examples of useful embodiments of the present invention and it will be apparent to one skilled in the art that the present invention may be carried out using a large number of variations of the devices, device components, methods steps set forth in the present description. As will be obvious to one of skill in the art, methods and devices useful for the present methods can include a large number of optional composition and processing elements and steps.
(131) As used herein and in the appended claims, the singular forms “a”, “an”, and “the” include plural reference unless the context clearly dictates otherwise. Thus, for example, reference to “a cell” includes a plurality of such cells and equivalents thereof known to those skilled in the art. As well, the terms “a” (or “an”), “one or more” and “at least one” can be used interchangeably herein. It is also to be noted that the terms “comprising”, “including”, and “having” can be used interchangeably. The expression “of any of claims XX-YY” (wherein XX and YY refer to claim numbers) is intended to provide a multiple dependent claim in the alternative form, and in some embodiments is interchangeable with the expression “as in any one of claims XX-YY.”
(132) Every device, system, combination of components, or method described or exemplified herein can be used to practice the invention, unless otherwise stated.
(133) Whenever a range is given in the specification, for example, a temperature range, a time range, or a composition or concentration range, all intermediate ranges and subranges, as well as all individual values included in the ranges given are intended to be included in the disclosure. It will be understood that any subranges or individual values in a range or subrange that are included in the description herein can be excluded from the claims herein.
(134) All patents and publications mentioned in the specification are indicative of the levels of skill of those skilled in the art to which the invention pertains. References cited herein are incorporated by reference herein in their entirety to indicate the state of the art as of their publication or filing date and it is intended that this information can be employed herein, if needed, to exclude specific embodiments that are in the prior art. For example, when composition of matter are claimed, it should be understood that compounds known and available in the art prior to Applicant's invention, including compounds for which an enabling disclosure is provided in the references cited herein, are not intended to be included in the composition of matter claims herein.
(135) As used herein, “comprising” is synonymous with “including,” “containing,” or “characterized by,” and is inclusive or open-ended and does not exclude additional, unrecited elements or method steps. As used herein, “consisting of” excludes any element, step, or ingredient not specified in the claim element. As used herein, “consisting essentially of” does not exclude materials or steps that do not materially affect the basic and novel characteristics of the claim. In each instance herein any of the terms “comprising”, “consisting essentially of” and “consisting of” may be replaced with either of the other two terms. The invention illustratively described herein suitably may be practiced in the absence of any element or elements, limitation or limitations which is not specifically disclosed herein.
(136) One of ordinary skill in the art will appreciate that devices, systems, and methods other than those specifically exemplified can be employed in the practice of the invention without resort to undue experimentation. All art-known functional equivalents, of any such devices and methods are intended to be included in this invention. The terms and expressions which have been employed are used as terms of description and not of limitation, and there is no intention that in the use of such terms and expressions of excluding any equivalents of the features shown and described or portions thereof, but it is recognized that various modifications are possible within the scope of the invention claimed. Thus, it should be understood that although the present invention has been specifically disclosed by preferred embodiments and optional features, modification and variation of the concepts herein disclosed may be resorted to by those skilled in the art, and that such modifications and variations are considered to be within the scope of this invention as defined by the appended claims.