METHODS OF EVALUATING TREATMENT OUTCOME IN HIGH GRADE SEROUS OVARIAN CANCER
20210231670 · 2021-07-29
Inventors
Cpc classification
C12Q2600/106
CHEMISTRY; METALLURGY
A61K45/06
HUMAN NECESSITIES
C12N15/113
CHEMISTRY; METALLURGY
C12Q2600/112
CHEMISTRY; METALLURGY
G01N2800/52
PHYSICS
International classification
A61K45/06
HUMAN NECESSITIES
C12N15/113
CHEMISTRY; METALLURGY
Abstract
Disclosed herein are method and compounds useful in the analysis, diagnosis, and treatment of high grade serous ovarian carcinoma (HGSOC). Also disclosed are methods, compounds, and compositions useful in regulating Chromobox 2 (CBX2) expression and therapies for stem-ness, anoikis escape, HGSOC dissemination, and HGSCO chemoresistance. Applicants have identified CBX2 expression as being significantly elevated in HGSOC cells and tissues compared to benign counterparts. Also disclosed is elevated CBX2 expression in HGSOC cell lines, as well as elevated CBX2 expression in cells that are forced to grown in suspension. Reducing CBX2 results in inhibition of anchorage-independent proliferation and potentiation of anoikis-dependent apoptosis, as well as re-sensitization of HGSOC cells to platinum-based chemotherapy.
Claims
1-3. (canceled)
4. A method of treating HGSOC in a patient, the method comprising: administering to the patient a therapeutically effective amount of a compound that inhibits and/or downregulates CBX2; contacting the compound with at least one cell in a tissue selected from ovarian, uterine, peritoneal, or fallopian tissue; downregulating CBX2 in that cell, thereby treating HGSOC in the patient.
5. The method of claim 4, wherein the compound comprises an antibody, siRNA, ribozyme, antisense, aptamer, peptidomimetic, small molecule, or any combinations thereof.
6. A method of determining and selecting a treatment for a patient with, or at risk of developing a cancer, the method comprising: obtaining a first sample from the patient comprising one or more cancerous or pre-cancerous cells; obtaining a second sample from the patient that is similar to the first sample but does not comprise cancerous or pre-cancerous cells; processing the first sample and the second sample to analyze at least one biomarker related to CBX2; quantifying the amount of biomarker in the first sample and the second sample, wherein if the amount of CBX2-related biomarker in the first sample is greater than the amount of CBX2-related biomarker in the second sample, the patient is identified as having aggressive or chemoresistant cancer; and selecting a chemotherapy for the patient that is not a platinum-based chemotherapeutic agent if the patient is identified as having aggressive or chemoresistant cancer, or a chemotherapy that is a platinum-based chemotherapeutic agent if the patient is not identified as having aggressive or chemoresistant cancer.
7. The method of claim 6, wherein the first sample is derived from ovarian, uterine, peritoneal, or fallopian tissue.
8. (canceled)
9. The method of claim 7, wherein the first sample includes one or more HGSOC cells.
10. The method of claim 9, wherein the biomarker comprises CBX2 protein or fragment thereof.
11. The method of claim 10, further comprising obtaining a second biomarker from each sample, wherein the second biomarker is not CBX2.
12. The method of claim 11, wherein the amount of biomarker is quantified by immunochemistry.
13. The method of claim 11, wherein the amount of biomarker is quantified by mass spectrometry.
14. The method of claim 9, wherein the biomarker is at least one nucleic acid.
15. The method of claim 14, wherein the biomarker is an mRNA sequence from the CBX2 gene.
16. The method of claim 6, wherein the platinum-based chemotherapeutic agent is cisplatin.
17-36. (canceled)
37. A method of inhibiting or reducing proliferation in a cancer cell, the method comprising: contacting the cell with a compound or molecule that inhibits CBX2 expression; allowing the compound to reduce the amount of CBX2 protein in the cell; thereby reducing or inhibiting proliferation of the cell compared to a control cell that is not contacted with the compound.
38. The method of claim 37, wherein the cell is in-vitro.
39. The method of claim 37, wherein the cell is in-vivo.
40. (canceled)
41. The method of claim 37, wherein the cell is a human cell.
42. The method of claim 37, wherein the compound is a nucleic acid.
43. The method of claim 42, wherein the compound is a short hairpin ribonucleic acid.
44. The method of claim 37, wherein the compound comprises two or more amino acids.
45. The method of claim 37, further comprising a step of contacting the cell with one or more chemotherapeutic agents before the reducing step.
Description
BRIEF DESCRIPTION OF THE DRAWINGS
[0011] The patent or application file contains at least one drawing executed in color. Copies of this patent or patent application publication with color drawing(s) will be provided by the Office upon request and payment of the necessary fee.
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DETAILED DESCRIPTION
[0022] Disclosed herein are studies showing that CBX2 is overexpressed in primary HGSOC tumors and that CBX2 protein is upregulated in HGSOC cells grown in an anchorage-independent fashion (forced suspension). In a primary human HGSOC tumor microarray high CBX2 expression was observed in a majority of specimens. Also shown is that the loss of CBX2 inhibits proliferation, reduces stemness, and increases cisplatin sensitivity. Also disclosed are methods of diagnosing advanced cases of HGSOC, HGSOC that may be resistant to one or more anti-cancer therapies, for example platinum-based chemotherapy, such as cisplatin therapy. Also disclosed are compounds and methods for treating a subject having advanced or chemoresistant HGSOC, wherein the compounds and methods reduce CBX2 expression in HGSOC cells and/or reduce dissemination of primary tumors.
[0023] High grade serous ovarian carcinoma (HGSOC) is often diagnosed at an advanced stage. Chromobox 2 (CBX2), a polycomb repressor complex subunit, plays an oncogenic role in other cancers, but little is known about its role in HGSOC. Applicants hypothesized that CBX2 upregulation promotes HGSOC via induction of a stem-like transcriptional profile and inhibition of anoikis. Examination of Gene Expression Omnibus (GEO) datasets and The Cancer Genome Atlas (TCGA) established that increased CBX2 expression conveyed chemoresistance and worse disease-free and overall survival. In primary HGSOC tumors, Applicants observed CBX2 expression was significantly elevated compared to benign counterparts. In HGSOC cell lines, forced suspension promoted CBX2 expression. Subsequently, CBX2 knockdown inhibited anchorage-independent proliferation and potentiated anoikis-dependent apoptosis. Furthermore, CBX2 knockdown re-sensitized cells to platinum-based chemotherapy. Forced suspension promoted increased ALDH activity and ALDH3A1 expression and CBX2 knockdown led to a decrease in both ALDH activity and ALDH3A1 expression. Investigation of CBX2 expression on a HGSOC tissue microarray revealed CBX2 expression was apparent in both primary and metastatic tissues. CBX2 is an important regulator of stem-ness, anoikis escape, HGSOC dissemination, and chemoresistance and potentially serves as a novel therapeutic target.
Discussion
[0024] Applicants have discovered that CBX2 is upregulated in HGSOC, high CBX2 expression portends poorer survival, and increased CBX2 expression correlates with platinum resistance. Applicants have also discovered that CBX2 is overexpressed in HGSOC primary tumors, as well as in cell lines that have escaped anoikis in suspension culture. Here, Applicants demonstrate that the loss of CBX2 is associated with decreased proliferation of HGSOC cells in multiple culture conditions, an increase in chemotherapy sensitivity, and a reduction in stem-like cells. Lastly, utilizing a HGSOC tissue microarray of advanced stage primary patient tumors Applicants found CBX2 protein expression was expressed in a majority of tumors.
[0025] CBX2 was found to be highly expressed in primary HGSOC tumors from seven patients. Clinically, three of these patients had more extensive peritoneal disease (Table 2) suggesting that CBX2 could serve as a predictive marker of advanced disease, reinforcing the potential clinical significance of CBX2. Moreover, examination of five patients with matched primary tumor, ascites-associated tumor cells, and distant metastasis revealed three of the five patients had an increase in CBX2 expression in distant metastasis/ascites-associated tumor cells compared to primary tumors. This highlights that CBX2 is potentially important in driving HGSOC progression, however there are indeed other contributing factors. Notably we observed that high expression of CBX2 correlated to a loss of an active tumor suppressor, FOXO3. This suggests that the downregulation of FOXO3 independent of CBX2 could drive tumor progression. As we have attempted to elucidate the mechanism behind CBX2-dependent activity we see common themes of increased proliferation and stem-like differentiation, which appeared to lead to a more aggressive and chemoresistant phenotype. The loss of CBX2 led to the loss of stemness measured through ALDH activity, ALDH3A1 expression, and SOX4 expression suggesting that reduced stemness is a major driver of these phenotypes. In addition, we linked the anoikis-induced autophagy, EMT, and apoptosis response to CBX2 expression. Further investigations will functionally evaluate the relationship between CBX2 and these key survival processes.
TABLE-US-00001 TABLE 2 Clinical characteristics of patient-derived protein lysates Label Specimen Pathologic Diagnosis Indication for surgery Clinical diagnosis FTE Normal HGSC, right fallopian Left adnexal mass, left Stage IIIC high grade (952) fallopian tube not involved hydroureter and serous ovarian tube hydronephrosis cancer, recurrent FTE Normal Endometrioid, Endometrial Stage IA (968) fallopian endometrial adenocarcinoma Endometrioid, tube adenocarcinoma, FIGO diagnosed on biopsy endometrial grade 1 adenocarcinoma, FIGO Grade I FTE Normal Lipoleiomyomata, Incidentally found 20 cm Benign pelvic mass (1008) fallopian bilateral fallopian tubes right adnexal mass on CT tube without histopathologic scan abnormality Benign Normal Uterus, cervix, and Malignant cells on pap Negative for (982) ovary bilateral ovaries and smear malignancy. fallopian tubes negative for malignancy HGSC Omentum HGSC Inflamed gallbladder, Stage IVB high grade (905) omental cake, and serous ovarian adnexal masses carcinoma, recurrent suspicious for ovarian cancer HGSC Tumor/ HGSC Adnexal masses, Stage IVB high grade (930) ovary concerning for ovarian serous ovarian cancer cancer, recurrent HGSC Tumor/ HGSC Pelvic mass, ascites, and Stage IIIC high grade (945) omentum/ carcinomatosis serous ovarian ovary concerning for ovarian cancer, recurrent cancer HGSC* Tumor/ HGSC Abdominal distension, Stage IIIC high grade (966) ovary bilateral adnexal masses serous ovarian cancer, recurrent HGSC Tumor/ HGSC Omental caking and Stage IIIC high grade (995) ovary adnexa masses serous ovarian cancer suspicious for ovarian cancer. HGSC Tumor/ HGSC Left adnexal mass, left Stage IIIC high grade (952) ovary hydroureter and serous ovarian hydronephrosis cancer, recurrent HGSC Tumor/ HGSC Bilateral adnexal masses Stage IIIC high grade (1003) ovary serous carcinoma of the fallopian tube Other Tumor/ Low grade serous Flank pain, adnexal Stage IV low grade histotype omentum carcinoma mass serous ovarian (953) cancer, recurrent Other Tumor/ Carcinosarcoma Pelvic mass in setting of Stage IVB histotype ovary carcinoma of unknown carcinosarcoma of the (950) origin ovary Other Tumor/ Carcinosarcoma Small bowel obstruction, Stage IIIC ovarian histotype ovary new diagnosis carcinosarcoma (991) carcinosarcoma Other Tumor/ Mucinous Ascites, bloating, cytology Stage IIIC mucinous histotype ovary adenocarcinoma, positive for adenocarcinoma of (993) intestinal type, primary adenocarcinoma the ovary tumor site: right ovary HGSC* Tumor/ HGSC Abdominal Stage IIIC high grade (966) ovary distension, serous ovarian bilateral adnexal masses cancer, recurrent Surgical CBX2 Label Surgical findings (when relevant) outcome status FTE Bilateral adnexal masses 4 × 5 cm on the right and Optimal Faint (952) 8 × 10 cm on the left. Significant retroperitoneal tumor debulking to burden, left greater than right necessitating <1 cm residual aggressive dissection and ligation of internal iliac disease artery to remove tumor. Per pathology report: right fallopian tube NOT INVOLVED with HGSOC FTE Mildly enlarged uterus, normal-appearing right Not applicable. Faint (968) fallopian tube and ovary. Previous surgically absent left fallopian tube and ovary. There was no disease noted in the pelvis. FTE Enlarged, approximately 20 × 20 cm retroperitoneal Optimal Faint (1008) mass arising within the broad ligament attached to the debulking uterus. Both ovaries and fallopian tubes were normal bilaterally. Extensive retroperitoneal fibrosis, necessitating complete ureterolysis. Benign Uterus slightly enlarged and globular. Significant intra- Not applicable Faint (982) abdominal adhesions. *No specific mention of ovaries in findings HGSC Extensive approximately 30 × 20 × 10 cm omental Optimal Bright (905) cake incorporating the infracolic and gastrocolic debulking to <1 cm, omentum, removed completely. Splenic hilar tumor however residual consisted and distal pancreatic surface tumor, removed of thousands of less completely with splenectomy and distal than 1 cm diffuse peritoneal and pancreatectomy. Diffuse intraabdominal mesenteric mesenteric nodules, as well and peritoneal masses up to 4 cm in size, debulked as areas of plaque which had with the use of argon beam to less than 1 cm been debulked to thickness of the removed plaques. Right adnexal less than 1 cm thickness, but mass approximately 8 cm. Diffuse peritoneal disease nonetheless consisted of measuring in the thousands, all debulked to less than plaque-like disease. 1 cm, discrete lesions or plaque-like in thickness areas. Gallbladder removed with thickened wall of the gallbladder, but no intrinsic luminal tumor. HGSC Bilateral adnexal masses, left with surface Poor candidate for primary Bright (930) involvement with a tumor process, approximately 10 debulking given extensive right cm in size. The right approximately 15 cm in size and diaphragmatic disease. mostly cystic. Small volume intraabdominal ascites, Underwent optimal approximately 50 cc. The right hemidiaphragm cytoreduction to infiltration approximately 3 to 4 cm thick and across an R0 after 3 cycles approximately 10 cm area making it difficult to remove of neoadjuvant without complete diaphragmatic resection. No chemotherapy obviously thickened omentum on laparoscopic assessment. Multiple small nodules ranging from 5 to 10 mm diffusely through the peritoneum. HGSC Approximately 2 L of ascites. Omental disease with Optimal Moderate (945) numerous peritoneal-based lesions, the majority less debulking to than 1 cm and those greater than 1 cm debulked to <1 cm, however less than 1 cm residual volume. Tumor roll in the innumerable miliary disease omentum approximately 15 cm × 9 cm and 4 cm, on the bilateral debulked to less than 1 cm residual. Bilateral adnexal diaphragms and peritoneal masses, approximately 10 cm on the left and surfaces approximately 4 cm on the right, densely adherent in the pelvis, with bilateral ureterolysis secondary to retroperitoneal fibrosis allowing resection of the ovaries. Anterior roll of tumor in the bladder peritoneum approximately 2 × 4 × 2 cm, completely removed. Tumor along the cecum and appendix resulting in removal of what appeared to be appendix involved with tumor, approximately 2 cm × 2 cm × 4 cm. Tumor mass in the lesser curvature of the stomach approximately 2 × 4 × 2 cm, removed completely. At the completion of the procedure, all disease was less than 1 cm, but did involve innumerable miliary disease on the subdiaphragms, as well as throughout all peritoneal surfaces. HGSC* Bilateral adnexal masses the right was approximately Optimal primary (966) 10 to 12 cm, left approximately 6 cm. There were debulking to R0 Moderate multiple small nodules within the omentum that appeared to be tumor. There was an approximately 2 cm nodule on the cecum that was sitting outside of the pelvis. There was a centimeter nodule sitting on the lower rectum in the pelvis, multiple subcentimeter nodules located in the lower abdomen, and the diaphragm on the right side was thinly coated with tumor that was ablated and bluntly dissected off with the Argon beam coagulation, so that this patient was optimally visually debulked to R0 disease. Pelvic and periaortic lymph node sampling was performed, for what appeared to be bulky disease in the periaortic lymph node bed. HGSC Omental mass 20 × 10 × 5 cm thick removed Optimal moderate (995) completely with omentectomy, bilateral adnexal debulking to <0.5 cm masses approximately 6 × 7 cm bilaterally, removed completely. Uterus slightly enlarged with an estimated weight of approximately 250 grams, removed completely, posterior carpets of peritoneal disease removed completely, with posterior cul-de-sac peritoneal stripping with argon beam to remove residual tumor in the pelvis down to less than 5 mm of residual disease. Bilateral retroperitoneal fibrosis necessitating dissection of the perirectal and perivesical spaces, and bilateral ureterolysis to allow complete resection of tumor and uterus secondary to the extensive dissection and the posterior cul-de-sac stripping. Diffuse 1 to 2 cm peritoneal disease debulked with excision or argon beam to less than 5 mm of residual, 5 mm thick, approximately 1 cm wide, scarred area at the dome of the liver in close approximation to the inferior vena cava which, was left in situ. HGSC Bilateral adnexal masses 4 × 5 cm on the right and Optimal Bright (952) 8 × 10 cm on the left. Significant retroperitoneal tumor debulking to burden, left greater than right necessitating <1 cm residual aggressive dissection and ligation of internal iliac disease artery to remove tumor. HGSC Extensive omental caking and adhered pelvis likely Optimal Faint- (1003) due to tumor with obliteration of the posterior cul-de- debulking to <0.5 cm moderate sac and the anterior cul-de-sac. Minimal normal tissue was able to be identified. Diaphragmatic studding and extensive miliary tumor disease along the mesentery of the small and large bowel. Otherwise, no obvious large tumor nodules were noted, and there was no lymphadenopathy. Other Bilateral adnexal masses as described on CT scan, Optimal primary Faint histotype with omental masses adherent to the uterus and the debulking to R0 (953) adnexal masses completely resected. The omentum had multiple areas of confluent tumor 3 to 4 × 4 cm in diameter, as well as multiple other peritoneal based lesions measuring anywhere between 1 to 4 cm and numbering approximately 100 or so. These were all completely removed and/or debulked with the use of the argon beam coagulator. There was also additional upper abdominal disease and splenic lesions completely resected by the team by consulting service. Debulking of the diaphragmatic lesion involved resection of full-thickness diaphragm, and the chest tube was inserted intraoperatively. Other Enlarged, approximately 8 to 10 cm, multicystic and Delayed optimal Negative histotype solid complex right adnexal mass that was densely primary debulking (950) adherent to the bladder, as well as an enlarged to R0 complex adnexal mass of the left ovary that was densely adherent to the left pelvic sidewall. Pelvic and periaortic lymph node beds were palpated, with no adenopathy demonstrated. Omentum had minimal tissue and fat, with no visible disease. The liver edges felt smooth, without evidence of disease bilaterally. There was an approximately 4 cm to 5 cm right diaphragmatic lesion that was removed by consulting surgeon. There was no other palpable or visible disease throughout the abdomen or pelvis. Other Omental cake, approximately 7 × 8 cm × Optimal Negative histotype approximately 4 cm thick, removed completely. Right debulking to 1-2 mm (991) adnexal mass, approximately 4 × 5 cm, with adhesions of the terminal ileum and cecum to the area of the mass causing upstream bowel obstruction, removed completely and debulked with Argon beam coagulator to less than 1 to 2 mm residual. Plaque of tumor in the deep pelvis reduced to approximately 1 to 2 mm plaque residual with the use of Argon beam coagulator. No clearly identifiable left ovary and tube; hence, the region of the left ovary in between the IP ligament above the ureter and to the pelvic sidewall was removed and sent together as left ovary and tube. Other Darksih brown ascites drained, approximately 9 liters. Optimal tumor debulking Faint histotype Enlarged bilateral adnexal masses. Uterus was small; (993) however, there was prior disease to the anterior cul- de-sac, and the bladder posteriorly in the cul-de-sac. There was evidence of some inflammation, as well as possible previous disease that was treated by chemotherapy. There was then an approximate 2 cm nodule on the omentum that was taken out with the omentectomy. There were also adhesions and thickening on the anterior abdominal wall. Liver felt completely smooth. Peritoneum inflamed. Small bowel and large bowel grossly normal. Appendix grossly normal. HGSC* Bilateral adnexal masses the right was approximately Optimal primary Moderate (966) 10 to 12 cm, left approximately 6 cm. There were debulking to R0 multiple small nodules within the omentum that appeared to be tumor. There was an approximately 2 cm nodule on the cecum that was sitting outside of the pelvis. There was a centimeter nodule sitting on the lower rectum in the pelvis, multiple subcentimeter nodules located in the lower abdomen, and the diaphragm on the right side was thinly coated with tumor that was ablated and bluntly dissected off with the Argon beam coagulation, so that this patient was optimally visually debulked to R0 disease. Pelvic and periaortic lymph node sampling was performed, for what appeared to be bulky disease in the periaortic lymph node bed.
[0026] CBX2 is a subunit of the polycomb repressor complex (PRC1), which has been shown to play a role in ovarian cancer. The enzymatic subunit or “writer” of PRC1, BMI-1, is considered to play a role in malignant transformation of multiple cancers, including ovarian cancer. In ovarian cancer, BMI-1 has been demonstrated to be associated with stem-ness and tumor initiation and serves as an independent predictor of poor outcome. Moreover, silencing of BMI-1 can lead to improved sensitivity to chemotherapy. This understanding of BMI-1 directly correlates and aligns with our CBX2 findings. Taken together with observations in other types of cancer, it seems likely that the PRC1 and specifically, CBX2, are novel therapeutic targets not only for HGSOC, but potentially for breast and prostate cancers.
[0027] CBX2 is considered to be an epigenetic “reader”. The existing literature suggests that targeting epigenetic “readers” is an effective strategy for targeted therapy. Bromodomain (acetyl-histone “reader”) inhibitors have the potential to suppress ALDH activity in ovarian cancer, providing evidence that targeting of an epigenetic reader may be able to alter the stem-like phenotype of a cell. One key example is JQ-1, a potent and selective inhibitor of the bromodomain and extra-terminal domain (BET) family of proteins, including BRD2, BRD3, and BRD4. Preliminary clinical data suggests that BET inhibitors may have therapeutic potential in human cancers. Furthermore, a Phase I clinical trial of an oral BET inhibitor demonstrated that this small molecule inhibitor was well tolerated in vivo, a critical development which paves the way for future targeting of epigenetic readers. This highlights that a “reader” or chromobox domain inhibitor could prove to be more effective with fewer adverse effects compared to inhibitors of epigenetic “writer” enzymes.
[0028] Disclosed herein, Applicants describe the role of CBX2 in promoting HGSOC disease progression. Mechanistically, CBX2 protects HGSOC against apoptosis and promotes a more stem-like phenotype. CBX2 is an epigenetic reader and is therefore targetable with a small molecule inhibitor. This work expands our understanding of the progression of HGSOC and identifies a novel therapeutic target.
[0029] Increased CBX2 protein in HGSOC (compared to fallopian tube epithelium, and other histotypes) is associated with poorer prognosis for the patient, repression of the FOXO3 tumor suppressor, and chemoresistance of HGSOC tumors and cells. CBX2 expression may be determined by various methods. In some embodiments, CBX2 protein expression may be assessed by densitometry of western immunoblot study. In many embodiments, increased expression may refer to CBX2 intensity that is greater than about 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%, 110%, 120%, 130%, 140%, 150%, 200%, 300%, 400%, 500%, 600%, 700%, 800%, or 900%, and less than about 1000%, 900%, 800%, 700%, 600%, 500%, 400%, 300%, 200%, 160%, 150%, 140%, 130%, 120%, 110%, 100%, 90%, 80%, 70%, 60%, 50%, 40%, 30%, or 20% compared to CBX2 in non-HGSOC cells. In other embodiments, relative CBX2 protein expression may be determined by other methods for quantitation of protein expression that are well-known to those of skill in the art. In many embodiments, CBX2 protein may be identified by an antibody specific to CBX2, for example anti-CBX2 antibody from Thermo Fisher Scientific (Cat # PA5-30996). Relative quantitation of CBX2 may be adjusted or standardized by comparing CBX2 amounts to various control proteins, for example actin. CBX2 expression may be quantified by various methods. In some embodiments, CBX2 protein is analyzed and/or quantified by flow cytometry or mass spectrometry.
[0030] Reducing CBX2 expression, for example by various knockdown or repression methods, significantly decreases viability of HGSOC cells. In many embodiments, repression or knockdown leads to decreased growth rate and/or anoikis (anchorage-independent cell death) in HGSOC cells.
[0031] Suppression of CBX2 expression can promote cell death in HGSOC cells and/or reduce their growth rate. Suppression may be accomplished by various methods. In some embodiments, CBX2 mRNA transcripts may be targeted by one or more compounds including, but not limited to, siRNA, ribozyme, antisense, aptamer, or other small molecule. In some embodiments, CBX2 repression or knockdown may be accomplished by targeting CBX2 protein with one or more of, but not limited to, an antibody, peptide, peptidomimetic, small molecule, or other compound. In one embodiment, CBX2 transcripts may be targeted by one or more short hairpin RNA (shRNA) molecules, or other types of RNAi well-known to those of skill in the art. In some embodiments, the shRNA molecules may comprise a sequence selected from GCCAAGGAAGCTCACTGCCAT (shCBX2 #1; SEQ ID NO:19) or ACGGAAAGGAACAGGAAGCAT (shCBX2 #2; SEQ ID NO:20). In many embodiments, HGSOC cells may be induced to reduce their growth rate or enter anoikis by reducing the amount of CBX2 transcripts by greater than about 5%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 95%, and less than about 100%, 90%, 80%, 70%, 60%, 50%, 40%, 30%, 20%, or 10% compared to HGSOC cells that have not been treated to reduce their CBX2 expression.
[0032] Patients having HGSOC cells with enhanced expression of CBX2 indicates that chemotherapy may be less effective. In some embodiments, the chemotherapy is a platinum-based compound, for example selected from one or more of cisplatin, carboplatin, and oxaplatin. In some embodiments, patients whose HGSOC tumor cells express high levels of CBX2 require an increased dosage of chemotherapeutic drug of greater than 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%, 150%, 200%, 250%, 300%, or 400%, and less than about 500%, 400%, 300%, 200%, 1500%, 100%, 90%, 80%, 70%, 60%, 50%, 40%, 30%, or 20%, compared to patients with tumors having lower CBX2 expression.
[0033] Repression of CBX2 expression can increase sensitivity of HGSOC cells to chemotherapy. In some embodiments, various therapies may be used to reduce expression of CBX2 in HGSOC cells and tumors. This may allow the use of various chemotherapeutic compounds to treat a patient positive for HGSOC and/or may allow the use of lower doses of chemotherapy to achieve a beneficial therapeutic effect, such as a reduction in tumor growth, lower cell growth, an increase in cancer cell death, or reduction in adverse side effects such as fatigue, hair loss, bruising, bleeding; infection; low red blood cell counts; nausea, etc.
[0034] CBX2 expression correlates to mRNA expression of genes associated with apoptosis, autophagy, and epithelial to mesenchymal transition (EMT). In many embodiments, the genes may include one or more of MYLK, NOG, and TNFSF10
[0035] Increased CBX expression results in driving cells toward a stem-like phenotype. In many embodiments, a stem-like phenotype may be evaluated by measuring aldehyde dehydrogenase (ALDH), where stemness is associated with increased ALDH activity. In many embodiments, CBX2 expression correlates with ALDH3A1 expression, and knockdown of CBX2 expression decreases ALDH3A1 expression, as well as the stem cell-associated transcription factor, SOX4.
Definitions
[0036] Biomarker may refer to any measurable indicator of the state of a cell, tissue, organ, or subject. In some embodiments, the biomarker is a molecule associated with a cell, for example a protein, peptide, or mRNA transcript. In some embodiments, the protein may be full-length or truncated protein, or a peptide fragment of a full-length protein. In some embodiments, a biomarker may be a nucleic acid, such as an mRNA transcript. In many embodiments, the biomarker may be an mRNA or protein associated with a given gene, for example CBX2, SOX4 MYLK, NOG, and TNFSF10. In most embodiments, the biomarker is a mammalian biomarker, for example human biomarker.
[0037] Subject includes any mammal, including mice, rats, guinea pigs, rabbits, dogs, cats, cows, horses, monkeys, and humans. A patient is a subject undergoing treatment or observation for a condition or disease, such as cancer. Cancer includes conditions or diseases of mammals characterized by uncontrolled cellular growth, hyperproliferative growth, hyperplasic growth, neoplastic growth, cancerous growth or oncogenic processes. Cancer may also refer to tumors, metastatic tissues or malignantly transformed cells, tissues, or organs, irrespective of histopathologic type or stage of invasiveness.
[0038] Chemotherapeutic agents include synthetic, natural, semisynthetic compounds and molecules useful in treating, killing, suppressing, or controlling cells that are displaying uncontrolled growth. Chemotherapeutic agents may reduce the proliferation of such cells and/or induce their death (programmed or non-programmed cell death). Chemotherapy refers to administration of such agents to a patient in need thereof. Chemoresistance may refer to the ability of a cell to avoid death or a decrease in proliferation in the presence of a chemotherapeutic agent.
[0039] Biological sample may refer to any sample removed, extracted, or derived from a patient or subject. In many embodiments a biological sample includes tissues, cells, protein, nucleic acids, etc. A biological sample may be processed for viewing or analysis, such as protein or nucleic acid quantitation. A control sample or reference sample may be a sample obtained from a similar subject, tissue, or cell that does not have, or is not expected to have, a disease or condition that is being assayed. In some embodiments, a reference sample may include samples from two or more subjects.
[0040] Therapeutically effective, as used herein refers to a therapeutic treatment, for example administration of a chemotherapeutic agent, that is adequate to accomplish a desired, expected, or intended result. When used in the context of treating a patient or subject, it may refer to an amount of agent which, when administered to a subject or patient for treating or preventing a disease, is an amount sufficient to effect such treatment or prevention of the disease or condition, and/or ameliorates at least one consequence of the disease or condition.
[0041] Inhibition or repression, as used herein means to lessen by some amount. For example, inhibition of gene expression by a compound or agent may cause the amount of protein or transcript in a given cell to be reduced relative to a cell that has not been contacted with the compound or agent.
[0042] Antibody may refer to any natural, synthetic, human, non-human, or humanized protein that may bind to an epitope on a target antigen. Antibodies may be mono or bi-specific. Antibodies may be multi or single chain proteins. In some embodiments the antibodies may include one or more Fc domains, Fv domains and/or CDRs. Antibodies may refer to peptides or peptide mimetics that bind to a target protein.
[0043] RNA interference (RNAi) includes any method of targeting expression of one or more genes by degrading mRNA transcribed from the gene. RNAi include siRNA, miRNA, shRNA, ribozyme, aptamers and the like, which typically contain some amount of sequence complementary to the gene transcript. RNAi techniques and methods are well known to those of skill in the art.
[0044] Peptide, polypeptide, and protein fragment may refer to natural or synthesized compounds and molecules containing natural or synthetic amino acids, amino acid equivalents and/or other non-amino groups. Peptides may be modified by replacement of one or more amino acids with related compounds, and/or modified by removing or adding elements, compounds, or molecules to one or more side chains or functional groups. The peptides can be linear or cyclic. Peptidemimetic refers to a compound or molecule that mimics a peptide in the peptide's ability to assume a three-dimensional structure on its own, or as a result of binding or contacting an epitope.
[0045] Metastasize may refer to the ability of a cancer cell to travel from the primary tumor, proliferate, and establish a second tumor. In some embodiments, a metastatic cell may be able to proliferate in suspension without attaching or adhering to a substrate, for example connective or other solid non-cancerous tissue.
Examples
Example 1—CBX2 is Upregulated in High Grade Serous Ovarian Cancer and is Associated with Poor Survival
[0046] We examined CBX2 expression in HGSOC in several publicly available datasets (Gene Expression Omnibus; GEO Dataset and The Cancer Genome Atlas; TCGA). High expression of CBX2 in TCGA HGSOC samples conveyed both a significantly worse disease-free survival (DFS; 11.7 vs. 17.6 months, Log-rank test p-value 0.00316) and overall survival (OS; 34 vs. 44.8 months, Log-rank test p-value 0.00116) (
[0047]
[0048] Ovarian surface and FTE are proposed to be the precursor cells for HGSOC; more recent data strongly support FTE as the predominant site of origin. Comparing CBX2 expression in ovarian surface epithelium or FTE to CBX2 expression in HGSOC, we observed that CBX2 was significantly higher in HGSOC (
Example 2—CBX2 is Upregulated in Tumor Cells in Suspension
[0049] HGSOC is unique compared to other solid types in its tendency to directly seed and disseminate throughout the peritoneal cavity, which requires an escape from anoikis, an anchorage-independent cell death. To determine whether CBX2 plays a role in HGSOC tumor cell's ability to survive without anchorage, or in a suspended setting, we examined the role of CBX2 on HGSOC growth in suspension. A forced suspension setting was achieved by plating cells on polyHEMA-coated tissue culture dishes (
[0050]
[0051] The high grade serous ovarian cancer cell lines OVCAR4, PEO1, and OVCAR8 were grown in adherent and suspended settings (
[0052] The panels of
Example 3—CBX2 Knockdown Inhibits Proliferation
[0053] To further elucidate the role of CBX2 in HGSOC, we evaluated the impact of CBX2 modulation in PEO1, OVCAR4, and OVCAR8 HGSOC cell lines. One of the two independent small hairpin RNAs (shRNA) specific for CBX2 or a control (shControl) were transduced into OVCAR4, PEO1, and OVCAR8 cells. CBX2 knockdown was confirmed via quantitative PCR (qPCR) and immunoblots, with approximately 60% knockdown in the presence of shCBX2#1 and 30% knockdown in the presence of shCBX2#2 (
Example 4—Tissue Microarray Supports a Role for CBX2 in Tumor Progression
[0054] In order to correlate the CBX2 in vitro findings to clinically relevant specimens, we utilized a tissue microarray (TMA) of HGSOC that recapitulated tumor progression. The TMA contained 24 primary tumors, with matched lymph node or distant metastases (Table 3). IHC was optimized and performed using a previously published CBX2 antibody, predicted to preferentially stain the nucleus (
TABLE-US-00002 TABLE 3 Clinical characteristics of matched tissue % Positive microarray % Positive Stain % Positive Age at Stage at Stain Lymph Stain diagnosis diagnosis Pathologic diagnosis PDS vs NACT Primary node Metastasis No clinical data 36.51% 22.09% 27.44% available No clinical data 20.68% 26.93% 33.06% available No clinical data No No 45.85% available primary primary 36 IV [Serous carcinoma, FIGO PDS (optimal) 34.30% 53.14% 10.75% Grade III per clinical documentation] 59 IIIC Papillary serous PDS (optimal) 50.23% 29.23% 24.21% carcinoma 84 IIIC Papillary serous PDS (optimal) 32.84% 23.91% 18.66% carcinoma 46 IIIC Papillary serous PDS (optimal) 14.40% 26.76% 15.55% carcinoma 73 IIIC Serous carcinoma, FIGO PDS (optimal) 6.96% n/a 18.72% Grade III No clinical data 32.60% 50.16% n/a available 67 IIIC Poorly differentiated PDS (optimal) 26.75% n/a 13.88% high-grade (FIGO III) multifocal serous cystadenocarcinoma 39 IV Moderate to poorly PDS (optimal) 30.24% 23.62% 30.78% differentiated serous papillary cystoadenocarcinoma (FIGO III) 44 IIIC Serous carcinoma, FIGO PDS (optimal) 2.77% 3.43% 11.49% grade III 50 IIIC Papillary serous PDS (optimal) 14.96% n/a 12.52% carcinoma, high grade 63 IIIC Poorly differentiated PDS (optimal) n/a 16.82% n/a serous carcinoma 54 IIIC Serous carcinoma (FIGO PDS (optimal) 33.47% 9.53% 31.03% Grade III) 74 IIIC Poorly differentiated PDS (optimal) n/a 13.43% 27.78% serous carcinoma, Grade III 66 IVA Serous carcinoma, PDS (optimal) 19.07% 34.20% 2.76% Grade III No clinical data 19.27% 27.81% 17.52% available 54 IIIC High grade serous PDS (optimal) 31.67% 28.51% 25.01% carcinoma 50 IIIC Poorly differentiated PDS (optimal) 27.17% 45.78% 25.94% serous carcinoma 60 IVB High grade serous PDS (suboptimal) 23.51% 18.58% 24.09% carcinoma 51 IIIC High grade serous PDS (optimal) 67.66% 38.10% 33.35% papillary carcinoma 66 IVB High grade serous PDS (optimal) 42.95% 50.99% 32.12% carcinoma 70 IVA Poorly differentiated PDS (suboptimal) 15.81% n/a 23.21% serous carcinoma 63 IIIC High grade serous PDS (suboptimal) 36.87% n/a 25.52% carcinoma
[0055] In parallel the TMA was stained with PAX8, a marker for Müllerian origin (
[0056]
[0057]
Example 5—CBX2 Expression is Associated with Chemoresistance
[0058] A majority of HGSOC patients treated with platinum-based (i.e., carboplatin) chemotherapy develop chemoresistance. A comparison of carboplatin sensitive HGSOC tumors to platinum resistant tumors demonstrates an increase in CBX2 in platinum resistant tumors (GSE1926) (
[0059] For example, CBX2 knockdown OVCAR4 cells had an 1050 of 12.68 μM in shCBX2#1 and 15.37 μM in shCBX2#2 compared to 38.67 μM for the control with intact CBX2 (
[0060] Panels of
[0061] The panels in
Example 5—CBX2 Regulation of Autophagy, Apoptosis, and EMT-Related Genes
[0062] Chemoresistance and dissemination of ovarian cancer cells are associated with changes in apoptosis, epithelial to mesenchymal transition (EMT), and autophagy. The PcG is an epigenetic complex that is responsible for transcriptional reprogramming through histone modification thus CBX2 could regulate a variety of genes. Utilizing HGSOC TOGA data we generated a list of potential CBX2 target genes through examination of mRNA correlations (Spearman r>0.15, 5838 genes). Utilizing published gene sets for EMT, autophagy, stemness, and apoptosis we cross-referenced the CBX2-associated genes (Table 4). CBX2-associated genes accounted for 18.8-28.4% of genes in the respective pathways (
[0063] The panels of
Example 6—Forced Growth in Suspension and Increased CBX2 Leads to a Stem-Like Phenotype
[0064] Several reports have demonstrated cells that survive in anchorage-independent conditions possess stem-like characteristics. We hypothesize that the polycomb repressor complex could be involved by inhibiting cellular differentiation and thus maintaining stemness. Applicants examined the CBX2-associated genes from TOGA against a published stemness gene set. We observed 25.2% of stemness-related genes overlapped with CBX2 genes. We next evaluated stemness by measuring aldehyde dehydrogenase (ALDH) activity both in the setting of suspension growth and CBX2 knockdown. We first determined whether placing OVCAR4 and OVCAR8 cells in suspension increased ALDH activity. We observed that in OVCAR4 cells ALDH activity was significantly increased in cells grown in suspension for 7 days (
[0065] The panels of
[0066] Next, we evaluated whether CBX2 knockdown impacted suspension-induced ALDH activity, we found that culturing OVCAR4 and OVCAR8 shCBX2 cells in suspension significantly inhibited ALDH activity compared to shControl cells (
TABLE-US-00003 TABLE 5 Pearson Spearman Gene Correlation (r) Correlation (r) ALDH1A1 −0.1 −0.13 ALDH2 −0.12 −0.14 ALDH3A1 0.19 0.21 ALDH3B1 −0.08 −0.12 ALDH1A3 −0.14 −0.15 ALDH3B1 −0.16 −0.12 ALDH3B2 −0.09 −0.12 ALDH9A1 −0.06 −0.07 ALDH3A2 −0.03 −0.04 TCGA Data (n = 489, Nature, 2011)
[0067] The panels of
Materials and Methods
1. Cell Culture
[0068] OVCAR4, PEO1, and OVCAR8 human high grade serous ovarian cancer cell lines were authenticated using small tandem repeat (STR) analysis (The University of Arizona Genetics Core) and routinely tested for mycoplasma with MycoLookOut (Sigma, St. Louis, Mo.). OVCAR8 and OVCAR4 cells were obtained from the Gynecologic Tumor and Fluid Bank (University of Colorado, Aurora, Colo.). PEO1 purchased from American Type Culture Collection. Cells were cultured in RPMI-1640 medium supplemented with 1% penicillin—streptomycin and 10% fetal bovine serum. The cell lines were maintained in 5% CO2 at 37° C.
2. Bioinformatic Database Analysis
[0069] Gene Expression Omnibus (GEO), hosted by the National Center for Biotechnology Information (NCBI), was queried for relevant databases. GSE18521, GSE10971, GSE1926, and GSE73064 were examined for relative CBX2 expression. The Cancer Genome Atlas (TCGA) Ovarian Serous Cystadenocarcinoma (Nature 2011) database was accessed via the cBIOPortal (www.cbioportal.org/) to evaluate the role of CBX2 in HGSOC disease-free and overall survival. Note: patient numbers for overall survival (n=485) and disease-free survival (n=396) differ due to the availability of data with TCGA. The database was queried for HGSOC identifying a total of 557 tumors with mRNA expression data and CBX2 upregulation was defined as CBX2 mRNA expression >1.5 standard deviation. RPPA data was assessed from the HGSOC Provisional dataset via the cBIOPortal.
3. Adherent and Suspension Environments
[0070] The adherent environment was created using standard tissue culture dishes. For suspension, tissue culture dishes were covered with 6 mg/ml poly-2-hydroxyethyl methacrylate (Poly-HEMA, Sigma) in 95% ethanol. The plates were incubated under sterile conditions to allow ethanol evaporation, followed by 30 min of ultraviolet light for sterilization. OVCAR4, PEO1, and OVCAR8 cells were cultured in each of these environments for 7 days.
4. Cisplatin Dose-Response
[0071] PEO1 and OVCAR4 cells were plated in 96-well plates in both adherent (4000 cells per well) and suspension (6000 cells per well) environments, as described above. These cells were treated over 24 h with increasing concentrations of Cisplatin (0.5-100 μM). 1640 RPMI media and media with 0.9% NaCl were used for control and vehicle control, respectively. Cell viability was assessed using the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTT) assay (Promega, Madison, Wis.). Means of at least five wells are reported and experiments were independently repeated in triplicate. Representative dose-response curves are shown.
5. Quantitative Reverse Transcription Polymerase Chain Reaction (RT-qPCR)
[0072] RNA was isolated using RNAeasy Plus Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's protocol. NanoDrop spectrophotometry was performed to confirm the concentration of extracted RNA. RT-qPCR was performed using the Luna Universal One-step RT-qPCR kit (New England BioLabs, Ipswich, Mass.) on a BioRad CFX96 or Applied Biosystems QuantStudio 6 Flex thermocycler using primers for specific target transcripts; 18s rRNA was examined as a housekeeping gene (Table 6).
TABLE-US-00004 TABLE 6 SEQ ID Gene Direction Sequence (5′-3′) NO: CBX2 Forward CGGCTGGTCCTCCAAACATAA 1 CBX2 Reverse CAGAACCGGAAGAGAGGCAA 2 18sRNA Forward AACTTTCGATGGTAGTCGCCG 3 18sRNA Reverse CCTTGGATGTGGTAGCCGTTT 4 ALDH3B1 Forward TACGCCTTCTCCAACAGCAG 5 ALDH3B1 Reverse GTCATGTGCATGAAGCCGTC 6 ALDH2 Forward GGTGTGGTCAACATTGTGCC 7 ALDH2 Reverse ATTACGCGGCCAATCTCAGT 8 ALDH3A1 Forward AAGAGGAGATCTTCGGGCCT 9 ALDH3A1 Reverse TAATCACCTTGTCGTTGCTGG 10 A MYLK Forward TCAACAGGGTCACCAACCAG 11 MYLK Reverse GGGGTCTGGGTATCCTTCAAT 12 NOG Forward TGGTGGACCTCATCGAACAC 13 NOG Reverse ATGAAGCCTGGGTCGTAGTG 14 TNFSF10 Forward TGCGTGCTGATCGTGATCTT 15 TNFSF10 Reverse CATTCTTGGAGTCTTTCTAAC 16 GAGC SOX4 Forward CCCAGCAAGAAGGCGAGTTA 17 SOX4 Reverse CATCGGCCAAATTCGTCACC 18
6. shRNA Knockdown
[0073] CBX2-specific shRNA were obtained from the University of Colorado Functional Genomics Facility (CBX2 #1: TRCN 0000020327 and CBX2 #2: TRCN 0000020328). An empty pLKO.1-puro was utilized as shControl (shCtrl). Plasmid isolation was performed using Plasmid Midi-Prep Kit (Qiagen). Twenty-four hours after seeding, cells were transfected with a total of 12 μg of DNA, including lentiviral packaging plasmids and the shRNA, in addition to 36 μg of polyethyenimine (PEI), for a 1:3 ratio of DNA to PEI. Cells were incubated overnight and transitioned to Dulbecco's Modified Eagle Media (DMEM) the following morning. Forty-eight hours after medium change, lentivirus was harvested. PEO1, OVCAR4, and OVCAR8 cells were seeded into six-well plates. When cells reached 80% confluence, they were transduced with lentivirus encoding CBX2-specific shRNAs or an shRNA control. A control well was maintained without virus to confirm puromycin selection. A 48-h puromycin selection was performed immediately following transduction. After medium change, cells were allowed to recover and then subjected to functional assays.
7. Immunoblot
[0074] Cell lysis was performed using radioimmunoprecipitation assay (RIPA) buffer (150 mM sodium chloride, Triton X-100, 0.5% sodium deoxycholate, 0.1% SDS [sodium dodecyl sulfate], 50 mM Tris, pH 8.0) supplemented with complete EDTA-free protease inhibitor cocktail (Roche), as well as NaF and NaV. Protein was quantified using bicinchoninic acid (BCA) protein assay (Thermo Fisher Scientific, Waltham, Mass.) and spectrophotometry. An 8% SDS polyacrylamide gel resolving gel was created with a 4% stacking gel. Twenty to thirty micrograms of total protein were loaded per well. Proteins were transferred to PVDF membrane using a Bio-Rad TransBlot Turbo. Western Blot analysis was performed using a rabbit primary antibody specific to CBX2 (Thermo Fisher Scientific, Cat # PA5-30996, 1:1000) and a mouse primary antibody against actin (Abcam, Cat # ab6276). CBX2 previously validated in Clermont, P. L. et al. Genotranscriptomic meta-analysis of the Polycomb gene CBX2 in human cancers: initial evidence of an oncogenic role. Br. J. Cancer 111, 1663-1672 (2014). Primary antibody incubation was performed overnight at 4° C. Secondary goat anti-rabbit green (LI-COR Biosciences, Lincoln, Nebr., Cat #926-32211) and goat anti-mouse red (LI-COR, Cat #926-68070) antibodies were applied the following morning for 1 h at room temperature. Bands were visualized using the LI-COR Odyssey Imaging System.
8. Proliferation Assays
[0075] For the Gaussia luciferase (gLuc) assay, the BioLux Gaussia Luciferase Assay kit (New England BioLabs) was utilized. OVCAR4 and PEO1 cells were grown in a 96-well plate, starting with 2000 cells per well. Media were collected every 24 h and stored in at −20°. For the assay and luminometer readings, the media were thawed and placed in a new 96-well plate. The assay was performed following the manufacturer's protocol and the relative light units were obtained using luminometry (GloMax) and charted with Prism software. Colony formation assays were performed in parallel using crystal violet staining. Briefly, cells were fixed (10% methanol/10% acetic acid/PBS), stained with crystal violet (0.4%) and washed with de-ionized water. Crystal violet was dissolved and absorbance was measured using a spectrophotometer (SpectraMaxM2e, Molecular Devices, San Jose, Calif.) at 590 nm and SoftMaxPro software. For the spheroid assay, 4000 cells were plated from a single cell suspension onto growth factor reduced Matrigel (Corning, Corning, N.Y.) and allowed to incubate for 12 days. Microscopic images were obtained and the diameter of each spheroid was measured in ImageJ (NIH). At least 50 spheroids were measured for each cell type and the diameters were averaged and graphed using Prism software.
9. Aldefluor Assays
[0076] Stemness was evaluated using the Aldefluor Kit (Cat #01700, Stemcell Technologies, Vancouver, Canada). OVCAR4 and OVCAR8 cells grown in adherent and suspension settings for 7 days were collected and prepared following manufacturer's protocol. An exception to the manufacturer's protocol was the reduction of Aldefluor reagent by 50%, using 2.5 μl per 1 ml of cell suspension. Flow cytometry was performed on a Gallios 561 Cytometer (BD Biosciences) with analysis at 488 nm
10. Gynecologic Tissue and Fluid Bank (GTFB)
[0077] The University of Colorado has an Institutional Review Board approved protocol (COMIRB #07-935) in place to collect tissue from gynecologic patients with both malignant and benign disease processes. All participants are counseled regarding the potential uses of their tissue and sign a consent form approved by the Colorado Multiple Institutional Review Board. The tissues are processed, aliquoted, and stored at −80° C.
11. Immunohistochemistry
[0078] Patient-derived xenograft tissue samples of HGSOC were fixed in formalin and embedded in paraffin. The University of Colorado Cancer Center Histology Core performed serial sectioning of the tissue at 5 micron thickness. For histopathologic examination, sections were de-paraffinized using xylene and hydrated in graded alcohol solutions. Antigen retrieval was performed using citrate buffer (pH 6.0) and boiling in pressurized steamer to 110° C. for 30 min. Endogenous peroxidase activity was quenched with 3% hydrogen peroxide in methanol for 20 min, followed by washing in TBS. A hydrophobic barrier was drawn around each section and tissues were blocked in 1% BSA in TBS for 30 min. TMA slides were single stained. Rabbit anti-CBX2 (Thermo Scientific, Cat # PA5-30996) was diluted to 1:50 in 1% BSA in TBS, applied to all sections, and incubated overnight at 4° C. Rabbit anti-PAX8 (Proteintech, Cat #10336-1-AP) was diluted to 1:200 in 1% BSA in TBS, applied to all sections, and incubated overnight at 4° C. An isotype control (Rabbit IgG) was incubated in parallel. The secondary antibody, anti-rabbit Dako Envision+System HRP Labeled Polymer (Dako Ref#K4003) was applied to the sections and allowed to incubate for 60 min at room temperature. Slides were subsequently washed with TBS and developed under the microscope using Liquid 3,3′-diaminobenzidine tetrahydrochloride (DAB)+Substrate Chromagen System (Agilent, Santa Clara, Calif.; Ref#K3468). Slides were counterstained with hematoxylin
12. Tissue Microarray
[0079] A previously constructed TMA comprised of matched primary, lymph node, and peritoneal metastases samples in duplicate from 24 patients with high grade serous carcinoma treated at the University of Colorado (COMIRB #14-0427), was stained with the CBX2 and PAX8 antibodies. With the aid of the University of Colorado Histology Core, the stained slides were scanned using Aperio imaging technology and annotated to highlight tumor based on PAX8 staining using ImageScope software. The TMA was then analyzed and scored by the University of Colorado Histology Core. Subsequently, two board-certified pathologists (M.D.P. and A.A.B.) manually reviewed and scored the CBX2 stain. Each sample was given a score for intensity (0, 1+, 2+, 3+) and percentage of cells staining (continuous variable). Only distinct nuclear staining of tumor cells was considered positive. From this data, H-scores were generated.
13. Apoptosis Assays
[0080] Following 72 h of growth in adherent and suspended states, OVCAR4 and PEO1 cells were harvested and washed in PBS. Alexa 488 Conjugated AnnexinV and Propidium Iodide (PI) (Thermo Fisher Scientific) staining were performed following manufacturer's protocol.
14. Statistical Analysis
[0081] Prism Graph Pad Prism software (v7) was utilized to generate graphs. Statistical tests include unpaired two-sided t-tests (comparing two groups), Log-rank (survival) or ANOVA (comparing greater than two groups) unless noted. A significance threshold was set at p<0.05, which was used for sample size determination. All experiments were performed in technical triplicates and biological triplicates unless noted. FloJo software (BD Biosciences, San Jose, Calif.) was used for analyzing flow cytometry data.
[0082] While multiple embodiments are disclosed, still other embodiments of the present invention will become apparent to those skilled in the art from the following detailed description. As will be apparent, the invention is capable of modifications in various obvious aspects, all without departing from the spirit and scope of the present invention. Accordingly, the detailed description is to be regarded as illustrative in nature and not restrictive.
[0083] All references disclosed herein, whether patent or non-patent, are hereby incorporated by reference as if each was included at its citation, in its entirety. In case of conflict between reference and specification, the present specification, including definitions, will control.
[0084] Although the present disclosure has been described with a certain degree of particularity, it is understood the disclosure has been made by way of example, and changes in detail or structure may be made without departing from the spirit of the disclosure as defined in the appended claims.