Maintenance Reservoir

Abstract

A maintenance reservoir for a digital dispense system. The maintenance reservoir includes an elongate trough disposed in a micro-well plate holder for holding fluid dispensed from a fluid droplet ejection head of a fluid droplet cartridge in the digital dispense system. The maintenance reservoir is disposed in the micro-well plate holder on at least one side of a micro-well plate and includes a position indicator to signify if the elongate trough is present or absent from the micro-well plate holder.

Claims

1. A maintenance reservoir for a digital dispense system, comprising an elongate trough disposed in a micro-well plate holder for holding fluid dispensed from a fluid droplet ejection head of a fluid droplet cartridge in the digital dispense system, wherein the maintenance reservoir is disposed in the micro-well plate holder on at least one side of a micro-well plate and the maintenance reservoir includes a position indicator to signify if the elongate trough is present or absent from the micro-well plate holder.

2. The maintenance reservoir of claim 1, wherein the elongate trough is removable from the micro-well plate holder.

3. The maintenance reservoir of claim 1, wherein the elongate trough has a length that corresponds to a length of a column of wells in the micro-well plate.

4. The maintenance reservoir of claim 1, wherein the elongate trough has a volume that is equal to a volume of fluid in a fluid droplet ejection cartridge.

5. The maintenance reservoir of claim 1, wherein the elongate trough has a rectangular cross-sectional shape.

6. A digital fluid dispense system comprising the maintenance reservoir of claim 1.

7. A digital dispense system for ejection of fluids onto a substrate, the digital dispense system comprising: a housing unit; a fluid droplet ejection cartridge disposed in the housing unit, the fluid droplet ejection cartridge comprising one or more fluids and being disposed on a fluid cartridge translation mechanism for moving the fluid droplet ejection cartridge back and forth in an X-direction within the housing unit; a micro-well plate holder for holding a micro-well plate below the fluid droplet ejection cartridge; and a removable maintenance reservoir disposed in the micro-well plate holder for periodic maintenance of the fluid droplet ejection cartridge.

8. The digital dispense system of claim 7, wherein the maintenance reservoir further comprises a position indicator to signify if the elongate trough is present or absent from the micro-well plate holder when sensed by a sensor of the digital dispense system.

9. The digital dispense system of claim 7, wherein the maintenance reservoir comprises an elongate trough has a length that corresponds to a length of a column of wells in the micro-well plate.

10. The digital dispense system of claim 7, wherein the maintenance reservoir has a volume that is equal to a volume of fluid in a fluid droplet ejection cartridge.

11. A method for maintaining a fluid droplet ejection cartridge in a digital fluid dispense system, the method comprising: providing a housing unit and a fluid droplet ejection cartridge disposed in the housing unit, the fluid droplet ejection cartridge comprising one or more fluids and being disposed on a fluid cartridge translation mechanism for moving the fluid droplet ejection cartridge back and forth in an X-direction within the housing unit; a micro-well plate holder for holding a micro-well plate below the fluid droplet ejection cartridge during fluid dispensing of the one or more fluids into wells of the micro-well plate; and a maintenance reservoir comprising an elongate trough disposed in the micro-well plate holder on at least one side of the micro-well plate holder, wherein the elongate trough comprises a position indicator to signify if the elongate trough is present or absent from the micro-well plate holder; periodically moving the fluid droplet ejection cartridge to a position adjacent to the elongate trough; and ejecting fluid from the fluid droplet ejection cartridge into the elongate trough for a predetermined period of time.

12. The method of claim 11, wherein the predetermined period of time is a period of time sufficient to clean nozzles on the fluid droplet ejection cartridge.

13. The method of claim 11, wherein the predetermined period of time is a period of time sufficient to empty fluid from the fluid droplet ejection cartridge into the elongate trough.

14. The method of claim 11, wherein the elongate trough is removable from the micro-well plate holder.

15. The method of claim 11, further comprising terminating fluid ejection from the fluid droplet ejection cartridge when a sensor of the digital fluid dispense system fails to detect the position indicator.

16. The method of claim 11, wherein the elongate trough has a length that corresponds to a length of a column of wells in the micro-well plate.

17. The method of claim 11, wherein the elongate trough has a volume that is equal to a volume of fluid in a fluid droplet ejection cartridge.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0014] FIGS. 1 and 2 are schematic views of prior art digital dispense systems showing maintenance stations therefor.

[0015] FIG. 3 is a perspective view of a digital dispense system according to an embodiment of the disclosure.

[0016] FIG. 4 is an elevational view, not to scale, of a back side of the digital dispense system of FIG. 3.

[0017] FIG. 5 is a plan view, not to scale, of a micro-well plate for use with a digital dispense system.

[0018] FIG. 6 is an elevational, not to scale, of the micro-well plate of FIG. 5.

[0019] FIG. 7 perspective view, not to scale, of the micro-well plate of FIG. 5 disposed on a prior art well plate holder.

[0020] FIG. 8 is a perspective view, not to scale, of a micro-well plate disposed on a well plate holder according an embodiment of the disclosure.

[0021] FIG. 9 is a plan view, not to scale, of the micro-well plate and well plate holder of FIG. 8.

[0022] FIG. 10 is a side view, not to scale, of the micro-well plate and well plate holder of FIG. 8.

[0023] FIG. 11 is a schematic view of a digital dispense system showing use of a micro-well plate and well plate holder according to an embodiment of the disclosure.

DETAILED DESCRIPTION OF EXEMPLARY EMBODIMENTS

[0024] With reference to FIGS. 3 and 4 there is shown a digital dispense device 10 that is activated by a switch 26 for accurately dispensing an amount of one or more fluids onto a substrate such as a micro-well plate from a fluid droplet ejection cartridge. The fluid droplet ejection cartridge moves in an x-direction across the micro-well plate as the well plate is moved through the digital dispense device in a y-direction by a tray movement mechanism 30. The tray movement mechanism 30 moves the micro-well plate on a micro-well plate holder through the digital dispense device 10 in a direction that is orthogonal to the direction of movement of the fluid droplet ejection cartridge during a fluid deposition process so that all of the wells of the micro-well plate may be filled with fluid. The well plate holder is designed to properly register the micro-well plate with respect to the digital dispense device 10. The well plate holder may also be attached to gears to move the well plate holder and micro-well plate in the y-direction through the digital dispense device. A rear opening 32 (FIG. 4) is provided in the housing 34 of the digital dispense device 10 so that all of the wells of the micro-well plate may be filled as the well-plate holder moves the micro-well plate through the digital dispense device.

[0025] The micro-well plate 40 used with the digital dispense device 10 is illustrated in FIGS. 5 and 6. The micro-well plate 40 includes a plurality of wells 42 disposed in rows 1-12 and columns A-H. A conventional micro-well plate holder 44 containing the micro-well plate 40 is illustrated in FIG. 7. Accordingly, in the prior art digital dispense device (FIGS. 1 and 2), maintenance of the ejection head on the fluid droplet ejection cartridge 12 only takes place in the permanent maintenance station 18. Hence, any fluid ejected from the fluid droplet ejection cartridge 12 that is not deposited onto the substrate 14 or into wells of a micro-well plate remain in the digital dispense device 10.

[0026] FIGS. 8-10 illustrate an improved micro-well plate holder 50 for the micro-well plate 40 that includes a maintenance reservoir in the shape of a elongate trough 52. The elongate trough 52 is disposed in the micro-well plate holder 50 on at least one side of the micro-well plate 40 and has a length that is sufficient to accept fluid from each of the rows 1-12 of the well plate 40. In the embodiment shown in FIG. 9, the elongate trough 52 is disposed on only one side of the well plate holder 50. However, a well-plate holder according to the disclosure may have an additional trough on an opposing side of the well plate holder 50. In another embodiment, elongate troughs may be disposed on three or four sides of the well plate holder 50. However, a single trough 52 may be sufficient to retain all of the fluid in a fluid droplet ejection cartridge for a variety of fluid deposition applications.

[0027] An important feature of the improved micro-well plate holder 50 and trough 52 is that there is a position indicator 54 at one end of the trough 52 that enables a sensor in the digital dispense device to determine if the trough 52 is present on the well-plate holder 50. In some embodiments, the position indicator is a raised area 54 or flat area of the trough 52 that can be sensed by a sensor in the digital dispense device. The sensor may be a lever that is raised when the micro-well plate holder 50 contains the trough 52 and the raised area 54 and that is lowered or falls when a micro-well plate holder does not contain the trough 52. In another embodiment, the sensor may be an optical sensor that is used to detect indicia that indicates the presence or absence of the trough 52. Any other suitable sensor, known to those skilled in the art may be used. The sensor may be configured by software in the digital dispense device to alert the user that the trough 52 is not present. The sensor may also be configured to alert a user that the micro-well plate holder 52 and micro-well plate have been removed from the device once the wells 42 have been filled, and the device is ready for a new micro-well plate 40 and new fluid deposition procedure.

[0028] In another embodiment illustrated in FIG. 10, the trough 52 described above is removably installed in a micro-well plate holder 60 in a recessed area 62 therein. Thus, when the trough 52 is full, the trough 52 may be removed from the micro-well plate holder 60 and a new trough 52 installed in the holder 60. In some embodiments, the elongate trough 52 may have a rectangular cross-sectional shape. However, any suitable cross-sectional shape of the trough may be used for holding waste fluid.

[0029] FIG. 11 is a schematic illustration of a digital dispense device 70 that does not require a permanent maintenance station. Accordingly, the device 70 may be used to fill wells 42 of a micro-well plate 40. The micro-well plate 40 is disposed on a well plate holder 60 that includes the removable trough 52. During a maintenance step, if the trough 52 is present as sensed by a sensor in the device 70, fluid 72 may be deposited into the trough 52 from the fluid droplet ejection cartridge 12 to remove any contamination or blockage of nozzles on the ejection head, and/or to empty the cartridge 12 of a particular fluid used to fill the wells 42 of the micro-well plate 40.

[0030] In another embodiment, the trough 52 may be included in an x-y table that is designed to hold and move the micro-well plate 40 for filling the wells 42 rather than using a well plate holder as described above. In the embodiments described herein, the trough and well plate holder may be made of a variety of materials including metals, plastics and ceramics. The materials for trough and well plate holder are desirably selected from materials that are compatible with the fluids being ejected from the fluid droplet ejection cartridges.

[0031] It is noted that, as used in this specification and the appended claims, the singular forms “a,” “an,” and “the,” include plural referents unless expressly and unequivocally limited to one referent. As used herein, the term “include” and its grammatical variants are intended to be non-limiting, such that recitation of items in a list is not to the exclusion of other like items that can be substituted or added to the listed items

[0032] For the purposes of this specification and appended claims, unless otherwise indicated, all numbers expressing quantities, percentages or proportions, and other numerical values used in the specification and claims, are to be understood as being modified in all instances by the term “about.” Accordingly, unless indicated to the contrary, the numerical parameters set forth in the following specification and attached claims are approximations that can vary depending upon the desired properties sought to be obtained by the present disclosure. At the very least, and not as an attempt to limit the application of the doctrine of equivalents to the scope of the claims, each numerical parameter should at least be construed in light of the number of reported significant digits and by applying ordinary rounding techniques.

[0033] While particular embodiments have been described, alternatives, modifications, variations, improvements, and substantial equivalents that are or can be presently unforeseen can arise to applicants or others skilled in the art. Accordingly, the appended claims as filed and as they can be amended are intended to embrace all such alternatives, modifications variations, improvements, and substantial equivalents.