Polyiodide resin powder for use with medical devices

Abstract

Disclosed is a system and method of treatment which provides a direct response to the treatment of pneumonia as related to infections using a powder comprising a polyiodide resin with broad spectrum bactericidal, fungicidal and virucidal properties. When the powder is applied directly to the lungs of a mammal an immediate contact kill of protozoa, bacteria, fungi and viruses that cause respiratory tract infections affecting the lungs of a mammal takes place. Also disclosed is an application of the polyiodide resin powder for use with personal protective equipment (PPE) including but not limited to face masks, face shields, and respirators.

Claims

1. A system to provide for an immediate contact kill of bacteria, fungi and viruses that cause infections affecting the lungs of a mammal, the system comprising: a polyiodide resin powder comprising a cationic resin having an anion that when reacted in the presence of iodine (I.sub.2 as a mineral) and Iodide (I.sup.−) salt (sodium or potassium iodide) forms I.sub.3.sup.−, I.sub.5.sup.−, and I.sub.7.sup.−; the polyiodide resin powder having a total weight of iodine ranging from about 45% to about 70% by weight of polyiodide complex depending on the introduction of I.sub.3.sup.−, I.sub.5.sup.−, and/or I.sub.7.sup.−; wherein the polyiodide resin powder comprises a mesh size of about 1 μm to about 150 μm; and a buffering agent to enable the polyiodide resin powder to be maintained at a desired pH in a ratio that allows for the control of the pH of the mixture in a wet environment.

2. The system of claim 1, wherein the polyiodide resin powder is introduceable into the lungs of a mammal via a delivery device.

3. The system of claim 2, wherein the delivery device comprises a dry product inhaler, a nebulizer or a ventilator.

4. The system of claim 1, wherein the pH ranges from about 3 to about 7.

5. The system of claim 1, wherein the ratio of polyiodide to buffering agent ranges from about 50% to about 100% of the total of the combined materials of the cationic resin and the buffering agent.

6. A method for providing a polyiodide resin powder to enable an immediate contact kill of bacteria, fungi and viruses that cause infections affecting the lungs of a mammal, the method comprising the steps of: providing a cationic resin comprising a positive charge and an anion with a negative charge; reacting the cationic resin in the presence of iodine (I.sub.2 as a mineral) and Iodide (I.sup.−) salt (sodium or potassium iodide) to allow for the formation of I.sub.3.sup.−, I.sub.5.sup.−, and I.sub.7.sup.− thereby forming a polyiodide resin having a total weight of iodine ranging from about 45% to about 70% by weight of polyiodide complex depending on the introduction of I.sub.3.sup.−, I.sub.5.sup.−, and/or I.sub.7.sup.−; processing said polyiodide resin to form a polyiodide resin powder having a mesh size of about 1 μm to about 150 μm; and adding a buffering agent to enable the pH of the polyiodide resin powder to be maintained in a ratio that allows for the control of the pH of the mixture in a wet environment.

7. The method of claim 6 further comprising the step of introducing the polyiodide resin powder into the lungs of a mammal via a delivery device.

8. The method of claim 7, wherein the delivery device comprises a dry product inhaler, a nebulizer or a ventilator.

9. The method of claim 6, wherein the desired pH ranges from about 3 to about 7.

10. The method of claim 6, wherein the ratio of polyiodide to buffering agent ranges from about 50% to about 100% of the total of the combined materials of the cationic resin and the buffering agent.

Description

BRIEF DESCRIPTION OF THE FIGURES

(1) FIG. 1. Release rates from previous studies.

(2) FIG. 2 Graph showing biological performance of latex/iodinated resin coated latex elastomers of the present disclosure against the challenge microorganism Pseudomonas aeruginosa, with re-inoculation every twenty-four hours (Report Number 901978).

(3) FIG. 3 Graph showing biological performance of latex/iodinated resin coated latex elastomers of the present disclosure against the challenge microorganisms S. aureus and E. Coli (Report Number 793489).

(4) FIG. 4 Table of compiled data exhibiting contact times and effectiveness.

DETAILED DESCRIPTION OF THE DISCLOSURE

Definitions

(5) Polyiodide—Molecular iodide of more than one iodine atom containing a net negative charge

(6) Antimicrobial—An agent that kills microorganisms or inhibits their growth.

(7) Ion-Exchange—An exchange of ions between two electrolytes or the exchange of ions of the same charge between an insoluble solid and a solution in contact with it or an electrolyte solution and a complex or solid state material.

(8) Biological Buffer—An organic substance that has a neutralizing effect on hydrogen ions.

(9) The antitoxic agent is preferably an antimicrobial agent, an antiviral agent, a biochemical agent or a reducing agent. The active agent preferably exerts a toxic effect on a diverse array of microorganisms and other pathogens and environmental toxins while not being toxic to the user. Preferably, the antitoxic agent comprises polyiodinated resin particles.

(10) Disinfectants are known in the art. One preferred demand disinfectant is polyiodinated resins. The particle sizes of the powders range from about 1 micron to about 150 microns. In some embodiments, the particle sizes range from about 5 microns to about 10 microns. Alternative sources of the polyiodinated resins may be used subject to meeting the same purity and physical conditions. Iodinated resins used in accordance with the present disclosure are referred to as polyiodinated resin.

(11) The base polymer used to manufacture such polyiodinated resins is a strong base anion exchange resin. These resins contain quaternary ammonium exchange groups which are bonded to styrene divinylbenzene polymer chains. Polyiodinated resins can be made with different percentages of iodine and may be used in accordance with the present disclosure. Different percentages of iodine in the polyiodinated resins will confer different properties to the resin, in particular, different levels of biocidal activity. The particular resin used is based on the desired application.

(12) A significant advantage of the present disclosure is that a relatively small amount of the antimicrobial agent need be applied in order to exert a significant toxic effect on a broad spectrum of pathogens.

(13) With regards to efficacy, the present disclosure has been tested against a robust organism Pseudomonas aeruginosa utilizing the following recognized standards: AATCC Method 100 (modified for twenty-four hour repeat insult testing) and ASTM E2149 (modified for twenty-four hour repeat insult testing). The test results showed an average reduction of greater than 10.sup.6 in bacterial count vs. untreated samples).

(14) As there was no testing protocol available to demonstrate the efficacy of the disclosed device as it relates to its kill capabilities, the time involved, and its long term efficacy, specific test protocols were developed in relation to the disclosed device. It is well-known in the industry of life sciences, testing protocols provide individual sets of instructions that allow for the recreation of a particular laboratory experiment. Protocols provide instructions for the design and implementation of experiments that include the safety bias, procedural equipment, statistical methods, reporting and troubleshooting standards for experiments. As disclosed herein, modifications were made to standardized test criteria (AATCC method 100 and ASTM E2149) which resulted in the development of specific protocols that allow for the evaluation and testing of the killing capability of the disclosed device over an extended time period of up to 96 hours and beyond.

(15) With regards to efficacy, the present disclosure has been tested against a robust organism Staphylococcus aureus utilizing the following recognized standards: AATCC Method 100 (modified for twenty-four hour repeat insult testing). The test results showed an average reduction of greater than 10.sup.6 in bacterial count vs. untreated samples).

(16) As an example, a horse having late stage pneumonia that was expected to expire within 24 hours was treated with the disclosed dry powder and was within 24 hours healthy and pneumonia free.

(17) The polyiodide resin powder when applied to the lungs of a mammal via a DPI, nebulizer or ventilator the antimicrobial agent is released or when used as a coating, printed application, or as an ingredient or additive such as on or in face masks or personal protective equipment (PPE). It is well known that PPE may include gloves, safety glasses and shoes, earplugs or muffs, hard hats, respirators, shields, coveralls, vests, isolation gowns and full body suits.

(18) One disclosed embodiment is a powder demand release antimicrobial contact disinfectant polyiodinated resin with the ability to be tailored to specific medical needs based on the iodine concentration of iodine in its various forms such as I.sub.3.sup.−, I.sub.5.sup.−, I.sub.7.sup.−.

(19) The powder demand release antimicrobial contact disinfectant polyiodinated resin has been proven to maintain its kill capabilities beyond 96 hours (repeated inoculation every 24 hours with >10.sup.7 Pseudomonas aeruginosa for the entire study) as referenced by test results done by Wuxi AppTec, a third party reference lab. The antimicrobial powder is capable of providing a high level of protection against microbes and other many biohazards, such as viruses, bacteria, fungi, and molds. In the disclosed embodiment, the polyiodinated resin particles advantageously have an average size within the range from about 5 μm to about 10 μm.

(20) As disclosed, the polyiodide resin powder begins with a pure cationic resin which is commercially available as a chloride (Cl.sup.−) as the anion. The anion exchange resin may be a whole series of possible polymers that are carbon based, but in the disclosed embodiment, the resin used is a commercially available styrene-divinylbenzene copolymer resin that has a quaternary ammonium cation as an integral part of the resin matrix. This can be described as resin with nitrogen (N) and carbon-based residues (R) attached to the resin, with the property of having a resin with a positive charge and a counter anion (Cl.sup.−) with a negative charge, to end up as a neutral complex.

(21) Typically, anion exchange resins are in the form of hydroxide (OH.sup.−) or chloride (Cl.sup.−). The hydroxide form can be further reacted with hydrochloric acid to form the chloride version of the resin as follows:
Resin-NR.sub.4.sup.+OH.sup.−+HCl=Resin-NR.sub.4.sup.+Cl.sup.−+H.sub.2O.

(22) This is further reacted in the presence of Iodine (I.sub.2 as a mineral) and Iodide (I.sup.−) salt (sodium or potassium iodide) to allow for the formation of I.sub.3.sup.−, I.sub.5.sup.−, and I.sub.7.sup.−. The initial reaction is [I.sub.2+I.sup.−=I.sub.3.sup.−], which upon excess I.sub.2 will react further to form I.sub.5− as in [I.sub.2+I.sub.3.sup.−=I.sub.5.sup.−], and which upon additional excess I.sub.2 will react further to form I.sub.7− as in [I.sub.2+I.sub.5.sup.−=I.sub.7.sup.−]. This is now referred to as the polyiodide resin in the disclosed system. Reactions are as follows:
Resin-NR.sub.4.sup.+Cl.sup.−+I.sub.3.sup.−=Resin-NR.sub.4.sup.+I.sub.3.sup.−+Cl.sup.−
Resin-NR.sub.4.sup.+Cl.sup.−+I.sub.5.sup.−=Resin-NR.sub.4.sup.+I.sub.5.sup.−+Cl.sup.−
Resin-NR.sub.4.sup.+Cl.sup.−+I.sub.7.sup.−=Resin-NR.sub.4.sup.+I.sub.7.sup.−+Cl.sup.−

(23) Various ratios of chemicals are combined to optimize the formation of the polyiodide versions above by adding an excess of the I.sub.2 and I.sup.− in appropriate proportions to substitute out the Cl.sup.− or other anions or halides based on the stoichiometry (ratio) of the reactants as given above. Multiple routes from chromatography to reactor pressures and heated fluid beds may be used to realize the end product in accordance with well-known manufacturing processes, with the variables of pressure, temperature and ratios.

(24) The reactor operates at elevated temperatures of above room temperature to the limits of the resin's thermal stability profile temperature and at pressures of one or more atmospheres of pressure. The process can be optimized to produce a batch of any size (subject to the reactor vessel size) in a matter of hours or within one day. The total weight of iodine in the polyiodinated resin formed from the process ranges about 45% to about 70% by weight of the polyiodide complex depending on the introduction of I.sub.3.sup.−, I.sub.5.sup.−, and/or I.sub.7.sup.−. By careful control of the ratios of the Resin based Chloride version of the resin and the I.sub.2 and I.sup.− ratios, mixtures ranging from the I.sub.3.sup.− through the I.sub.7.sup.− versions and mixtures in between can be produced. Careful control of specific ratios of reactants can yield specific versions, but are typically reaction mixtures favoring one of the polyiodides over the others. For example, if I.sub.3.sup.− is introduced, the resulting polyiodinated resin comprises about 45% by weight of the polyiodide complex. If I.sub.5.sup.− is introduced, the resulting polyiodinated resin comprises about 62% (by weight of the polyiodide complex. If I.sub.7.sup.− is introduced, the resulting polyiodinated resin comprises about 69% by weight of the polyiodide complex.

(25) The resulting polyiodide resin is then ground to about 5 μm to about 10 μm thereby forming the polyiodide resin powder. Yields at or near 100% are possible, but typically due to manufacturing loses and limits may be less than 100%.

(26) Buffering agent can be added to maintain the desired pH, subject to the specific buffering agent that is used, in a ratio that allows for the control of the pH of the mixture in a wet environment (such as tissue or lungs) to be in the range of 3 to 7 pH units. Although any ratio of polyiodide to buffering agent can be used in the range of 10% to 100% of the polyiodide, typically the dominate agent is the polyiodide in the range of 50% to 100% of the total of the combined materials of the polyiodide styrene-divinylbenzene copolymer resin and the buffer agent.

(27) Some examples for medical grade buffering agents that may be used are 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), 2-(N-morpholino)ethanesulfonic acid (MES), 3-(N-morpholino)propanesulfonic acid (MOPS) and citrates, however others may be suitable.