HETEROAROMATIC CARBOXAMIDE DERIVATIVES AS PLASMA KALLIKREIN INHIBITORS

Abstract

Disclosed are heteroaromatic carboxamides of formula (I),

##STR00001##

wherein Y, R, and Ar are as defined herein, and pharmaceutically acceptable salts thereof. Also disclosed are the use of the compounds of formula (i) for the treatment of diseases which can be influenced by the inhibition of plasma kallikrein.

Claims

1. A compound of formula (I) ##STR00901## wherein Y is selected from the group consisting of ##STR00902## each of which is substituted with 1 or 2 independent substituents R.sup.1; R is selected from the group consisting of saturated 6- to 12-membered bicyclic ring systems containing 1 to 2 N atoms as ring members and optionally 1 ring member selected from the group consisting of C═O, O, S, S═O and SO.sub.2, provided that the ring systems do not contain any heteroatom-heteroatom bonds between ring members, wherein said ring systems are attached via an N atom to the group Y in formula (I), and wherein said ring systems are optionally substituted with 1 to 6 F and optionally substituted with 1 to 3 substituents selected from the group consisting of C.sub.1-3-alkyl, CN, HO—C.sub.1-3-alkylene, OH, and C.sub.1-3-alkyl-O; Ar is selected from the group consisting of 5-membered heteroaryls, containing 1 to 4 N atoms or containing 1 O or S atom or containing 1 to 2 N atoms and 1 O or S atom, and 9-membered heteroaryls, consisting of a 5-membered ring fused to a 6-membered ring and containing 1 to 4 N atoms, wherein said heteroaryls are attached to the carbonyl group in formula (I) via a C atom of the 5-membered ring and to the CH.sub.2 group in formula (I) via a non-adjacent C or N atom of the 5-membered ring, and wherein said heteroaryls are optionally substituted with 1 substituent R.sup.3; R.sup.1 is selected from the group consisting of H, halogen, C.sub.1-4-alkyl optionally substituted with 1 to 5 F, C.sub.3-4-cycloalkyl optionally substituted with 1 CH.sub.3, CN or OH group, CN, O—C.sub.1-3-alkyl optionally substituted with 1 to 5 F, C.sub.1-3-alkyl optionally substituted with 1 substituent selected from the group consisting of CN, OH, and O—C.sub.1-3-alkyl; R.sup.3 is selected from the group consisting of F, Cl, Br, CN, C.sub.1-4-alkyl optionally substituted with 1 to 5 F, C.sub.3-4-cycloalkyl, HO—C.sub.1-4-alkylene, C.sub.1-3-alkyl-O—C.sub.1-3-alkylene, and O—C.sub.1-4-alkyl optionally substituted with 1 to 5 F; or a pharmaceutically acceptable salt thereof.

2. The compound according to claim 1, wherein Y is selected from the group consisting of ##STR00903## each of which is substituted with 1 or 2 independent substituents R.sup.1 and wherein the bonds with asterisk indicate the sites of attachment of R and the CH.sub.2 group of formula (I); or a pharmaceutically acceptable salt thereof.

3. The compound according to claim 1, wherein R is selected from the group consisting of 5-azaspiro[2.3]hexane, 2-azaspiro[3.3]heptane, 5-azaspiro[2.4]heptane, 6-azaspiro[3.4]octane, 3-azabicyclo[3.1.0]hexane, 3-azabicyclo[3.2.0]heptane, octahydrocyclopenta[c]pyrrole, 6-azaspiro[2.5]octane, 5-azaspiro[2.5]octane, 7-azaspiro[3.5]nonane, 3-azabicyclo[4.1.0]heptane, 3-azabicyclo[3.1.1]heptane, 6-oxa-3-azabicyclo[3.1.1]heptane, and 3-azabicyclo[3.2.1]octane, each of which is attached via the N atom to the group Y in formula (I) and each of which is optionally substituted with one substituent selected from the group consisting of F, CH.sub.3, CN, CH.sub.2OH, OH, and OCH.sub.3, and each of which is optionally substituted with one additional substituent selected from the group consisting of F and CH.sub.3; or a pharmaceutically acceptable salt thereof.

4. The compound according to claim 1, wherein Ar is selected from the group consisting of ##STR00904## and the tautomers thereof, each of which is optionally substituted with 1 substituent R.sup.3 and wherein the bonds with asterisk and parentheses indicate the sites of attachment of the groups C═O and CH.sub.2 of formula (I); or a pharmaceutically acceptable salt thereof.

5. The compound according to claim 1, wherein R.sup.1 is selected from the group consisting of H, F, Cl, Br, C.sub.1-2-alkyl optionally substituted with 1 to 5 F or with 1 CN, OH or O—C.sub.1-2-alkyl group, C.sub.3-4-alkyl optionally substituted with 1 CN or OH group, C.sub.3-4-cycloalkyl optionally substituted with 1 CH.sub.3, CN or OH group, O—C.sub.1-2-alkyl optionally substituted with 1 to 5 F; or a pharmaceutically acceptable salt thereof.

6. The compound according to claim 1, wherein R.sup.3 is selected from the group consisting of F, Cl, Br, CN, C.sub.1-3-alkyl optionally substituted with 1 to 3 F, HO—C.sub.1-4-alkylene, C.sub.1-2-alkyl-O—C.sub.1-2-alkylene, and O—C.sub.1-2-alkyl optionally substituted with 1 to 3 F; or a pharmaceutically acceptable salt thereof.

7. The compound according to claim 1, wherein the stereochemistry of the compound is according to formula (I.1) ##STR00905## or a pharmaceutically acceptable salt thereof.

8. A pharmaceutically acceptable salt of the compound according to claim 1.

9. A pharmaceutical composition comprising one or more compounds according to claim 1, or pharmaceutically acceptable salts thereof, optionally together with one or more inert carriers and/or diluents.

10. A pharmaceutical composition comprising one or more compounds according to claim 1, or pharmaceutically acceptable salts thereof, and one or more additional therapeutic agents, optionally together with one or more inert carriers and/or diluents.

11. The pharmaceutical composition according to claim 10, wherein the one or more additional therapeutic agents are selected from the group consisting of antidiabetic agents, agents for the treatment of overweight and/or obesity, agents for the treatment of high blood pressure, heart failure and/or atherosclerosis and agents for the treatment of ocular diseases.

12. A method for treating an ocular disease, the method comprising administering to the patient a pharmaceutically effective amount of one or more compounds according to claim 1, or pharmaceutically acceptable salts thereof.

13. The method according to claim 12, wherein the ocular disease is selected from the group consisting of diabetic macular edema, age-related macular degeneration and choroidal neovascularization.

14. A compound selected from the group consisting of ##STR00906## or a salt thereof.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0060] The present invention discloses novel heteroaromatic carboxamide derivatives, which are effective plasma kallikrein (PKK) inhibitors and possess suitable pharmacological and pharmacokinetic properties to use them as medicaments for the treatment of diseases and/or conditions that may be influenced by PKK inhibition, including but not limited to diabetic complications, ocular diseases and edema-associated diseases, in particular diabetic macular edema, age-related macular degeneration, choroidal neovascularization, hereditary angioedema, and brain edema after stroke.

[0061] The compounds of the present invention may provide several advantages, such as enhanced potency, high metabolic and/or chemical stability, high selectivity, safety and tolerability, enhanced solubility, enhanced permeability, desirable plasma protein binding, enhanced bioavailability, improved pharmacokinetic profiles, and the possibility to form stable salts.

Compounds of the Invention

[0062] In a first aspect of the present invention, it is found that compounds of formula (I)

##STR00009##

wherein Y, R, and Ar are defined as hereinbefore and hereinafter, are potent inhibitors of PKK and exhibit favorable properties with regard to selectivity, safety and tolerability, metabolic and/or chemical stability, pharmacokinetic and physicochemical characteristics, solubility, permeability, plasma protein binding, bioavailability and/or the possibility to form stable salts. In particular, they provide an advantageous combination of high potency on human PKK and significant selectivity, e.g. vs. various serine proteases, such as human tissue kallikrein 1 (TK1), as well as adequate solubilities at physiologically relevant pH values and high metabolic stabilities. In addition, advantageous safety features, such as low potential of mutagenicity, low inhibition of cytochrome P450 (CYP) enzymes like CYP3A4 and CYP2C8, and low propensity for mechanism based inhibition of CYP3A4, are exhibited.

[0063] Therefore, the compounds of formula (I), as defined hereinbefore or hereinafter, or pharmaceutically acceptable salts thereof are expected to be useful in the treatment of diseases and/or conditions that can be influenced by PKK inhibition.

[0064] Thus, according to one aspect of the present invention, a compound of formula (I)

##STR00010##

wherein Y, R, and Ar are defined as hereinbefore or hereinafter, is provided
as well as the isoforms, tautomers, stereoisomers, metabolites, prodrugs, solvates, hydrates, cocrystals, and the salts thereof, particularly the pharmaceutically acceptable cocrystals and salts thereof.

[0065] Unless otherwise stated, the groups, residues and substituents, particularly Y, R, Ar, R.sup.1, and R.sup.3 are defined as hereinbefore and hereinafter. Some preferred meanings of the substituents Y, R, Ar, R.sup.1, and R.sup.3 as well as of the stereochemistry of the compounds of formula (I) will be given hereinafter as embodiments of the invention. Any and each of these definitions and embodiments may be combined with one another.

[0066] According to one embodiment, Y is selected from the group Y-G1 consisting of

##STR00011##

each of which is substituted with 1 or 2 independent substituents R.sup.1.

[0067] According to another embodiment, Y is selected from the group Y-G2 consisting of

##STR00012##

each of which is substituted with 1 or 2 independent substituents R.sup.1 and
wherein the bonds with asterisk indicate the sites of attachment of R and the CH.sub.2 group of formula (I).

[0068] According to another embodiment, Y is selected from the group Y-G3 consisting of

##STR00013##

each of which is substituted with 1 or 2 substituents R.sup.1 and
wherein the bonds with asterisk and parentheses indicate the sites of attachment of R and the CH.sub.2 group of formula (I).

[0069] According to another embodiment, Y is selected from the group Y-G4 consisting of

##STR00014##

each of which is substituted with 1 substituent R.sup.1 and
wherein the bonds with asterisk and parentheses indicate the sites of attachment of R and the CH.sub.2 group of formula (I).

[0070] According to another embodiment, Y is selected from the group Y-G5 consisting of

##STR00015##

each of which is optionally substituted with 1 additional substituent R.sup.1 and
wherein the bonds with asterisk and parentheses indicate the sites of attachment of R and the CH.sub.2 group of formula (I).

[0071] According to another embodiment, Y is selected from the group Y-G6 consisting of

##STR00016##

which is optionally substituted with one additional substituent R.sup.1 and
wherein the bonds with asterisk and parentheses indicate the sites of attachment of R and the CH.sub.2 group of formula (I).

[0072] According to another embodiment, Y is selected from the group Y-G7 consisting of

##STR00017##

wherein the bonds with asterisk and parentheses indicate the sites of attachment of R and the CH.sub.2 group of formula (I).

[0073] According to one embodiment, R is selected from the group R-G1 consisting of

saturated 6- to 12-membered bicyclic ring systems containing 1 to 2 N atoms as ring members and optionally 1 ring member selected from the group consisting of C═O, O, S, S═O and SO.sub.2,
provided that the ring systems do not contain any heteroatom-heteroatom bonds between ring members,
wherein said ring systems are attached via an N atom to the group Y in formula (I), and
wherein said ring systems are optionally substituted with 1 to 6 F and optionally substituted with 1 to 3 substituents selected from the group consisting of C.sub.1-3-alkyl, CN, HO—C.sub.1-3-alkylene, OH, and C.sub.1-3-alkyl-O.

[0074] According to another embodiment, R is selected from the group R-G2 consisting of

saturated 6- to 10-membered bicyclic ring systems containing 1 N atom and optionally 1 O atom as ring members,
wherein the ring systems are attached via the N atom to the group Y in formula (I), and
wherein the ring systems are optionally substituted with 1 substituent selected from the group consisting of F, C.sub.1-3-alkyl (preferably CH.sub.3), CN, HO—C.sub.1-3-alkylene (preferably HOCH.sub.2), OH, and C.sub.1-3-alkyl-O— (preferably CH.sub.3O), and
wherein the ring system is optionally additionally substituted with one substituent selected from the group consisting of F and CH.sub.3.

[0075] According to another embodiment, R is selected from the group R-G3 consisting of

5-azaspiro[2.3]hexane, 2-azaspiro[3.3]heptane, 5-azaspiro[2.4]heptane, 6-azaspiro[3.4]octane, 3-azabicyclo[3.1.0]hexane, 3-azabicyclo[3.2.0]heptane, octahydrocyclopenta[c]pyrrole, 6-azaspiro[2.5]octane, 5-azaspiro[2.5]octane, 7-azaspiro[3.5]nonane, 3-azabicyclo[4.1.0]heptane, 3-azabicyclo[3.1.1]heptane, 6-oxa-3-azabicyclo[3.1.1]heptane, and 3-azabicyclo[3.2.1]octane,
each of which is attached via the N atom to the group Y in formula (I) and
each of which is optionally substituted with one substituent selected from the group consisting of F, CH.sub.3, CN, CH.sub.2OH, OH, and OCH.sub.3, preferably consisting of F and CH.sub.3, and
each of which is optionally substituted with one additional substituent selected from the group consisting of F and CH.sub.3.

[0076] According to another embodiment, R is selected from the group R-G4 consisting of

##STR00018##

[0077] According to another embodiment, R is selected from the group R-G5 consisting of

##STR00019##

[0078] According to another embodiment, R is selected from the group R-G6 consisting of

##STR00020##

[0079] According to another embodiment, R is selected from the group R-G7 consisting of

##STR00021##

[0080] According to another embodiment, R is selected from the group R-G8 consisting of

##STR00022##

Ar:

[0081] According to one embodiment, Ar is selected from the group Ar-G1 consisting of

5-membered heteroaryls, containing 1 to 4 N atoms or containing 1 O or S atom or containing 1 to 2 N atoms and 1 O or S atom, and 9-membered heteroaryls, consisting of a 5-membered ring fused to a 6-membered ring and containing 1 to 4 N atoms,
wherein said heteroaryls are attached to the carbonyl group in formula (I) via a C atom of the 5-membered ring and to the CH.sub.2 group in formula (I) via a non-adjacent C or N atom of the 5-membered ring, and
wherein said heteroaryls are optionally substituted with 1 substituent R.sup.3.

[0082] According to another embodiment, Ar is selected from the group Ar-G2 consisting of

5-membered heteroaryls, containing 1 to 3 N atoms or containing 1 O or S atom or containing 1 N atom and 1 O or S atom, and 9-membered heteroaryls, consisting of a 5-membered ring fused to a 6-membered ring and containing 1 to 3 N atoms,
wherein said heteroaryls are attached to the carbonyl group in formula (I) via a C atom of the 5-membered ring and to the CH.sub.2 group in formula (I) via a non-adjacent C or N atom of the 5-membered ring, and
wherein said heteroaryls are optionally substituted with 1 substituent R.sup.3.

[0083] According to another embodiment, Ar is selected from the group Ar-G3 consisting of

##STR00023##

and the tautomers thereof,
each of which is optionally substituted with 1 substituent R.sup.3 and
wherein the bonds with asterisk and parentheses indicate the sites of attachment of the groups C═O and CH.sub.2 of formula (I).

[0084] According to another embodiment, Ar is selected from the group Ar-G4 consisting of

##STR00024##

each of which is optionally substituted with 1 substituent R.sup.3 and
wherein the bonds with asterisk and parentheses indicate the sites of attachment of the groups C═O and CH.sub.2 of formula (I).

[0085] According to another embodiment, Ar is selected from the group Ar-G5 consisting of

##STR00025##

which is optionally substituted with 1 substituent R.sup.3 and
wherein the bonds with asterisk and parentheses indicate the sites of attachment of the groups C═O and CH.sub.2 of formula (I).

[0086] According to another embodiment, Ar is selected from the group Ar-G6 consisting of

##STR00026##

which is optionally substituted with 1 substituent R.sup.3 and
wherein the bonds with asterisk and parentheses indicate the sites of attachment of the groups C═O and CH.sub.2 of formula (I).

[0087] According to another embodiment, Ar is selected from the group Ar-G7 consisting of

##STR00027##

[0088] According to another embodiment, Ar is selected from the group Ar-G8 consisting of

##STR00028##

which is optionally substituted with 1 substituent R.sup.3 and
wherein the bonds with asterisk and parentheses indicate the sites of attachment of the groups C═O and CH.sub.2 of formula (I).

[0089] According to another embodiment, Ar is selected from the group Ar-G9 consisting of

##STR00029##

[0090] According to another embodiment, Ar is selected from the group Ar-G10 consisting of

##STR00030##

[0091] According to another embodiment, Ar is selected from the group Ar-G11 consisting of

##STR00031##

which is optionally substituted with 1 substituent R.sup.3 and
wherein the bonds with asterisk and parentheses indicate the sites of attachment of the groups C═O and CH.sub.2 of formula (I).

R.SUP.1.:

[0092] According to one embodiment, R.sup.1 is selected from the group R.sup.1-G1 consisting of

H, halogen, C.sub.1-4-alkyl optionally substituted with 1 to 5 F, C.sub.3-4-cycloalkyl optionally substituted with 1 CH.sub.3, CN or OH group, CN, O—C.sub.1-3-alkyl optionally substituted with 1 to 5 F, C.sub.1-3-alkyl optionally substituted with 1 substituent selected from the group consisting of CN, OH, and O—C.sub.1-3-alkyl.

[0093] According to another embodiment, R.sup.1 is selected from the group R.sup.1-G2 consisting of H, F, Cl, Br, C.sub.1-2-alkyl optionally substituted with 1 to 5 F or with 1 CN, OH or O—C.sub.1-2-alkyl group, C.sub.3-4-alkyl optionally substituted with 1 CN or OH group, C.sub.3-4-cycloalkyl optionally substituted with 1 CH.sub.3, CN or OH group, O—C.sub.1-2-alkyl optionally substituted with 1 to 5 F.

[0094] According to another embodiment, R.sup.1 is selected from the group R.sup.1-G3 consisting of

H, F, Cl, Br, CH.sub.3, CH.sub.2CH.sub.3, CH.sub.2CH.sub.2CH.sub.3, CH.sub.2CH(CH.sub.3).sub.2, cyclopropyl, cyclobutyl, CHF.sub.2, CF.sub.3, CN, 1-cyanocycloprop-1-yl, CH.sub.2CN, C(CH.sub.3).sub.2CN, CH.sub.2OH, CH.sub.2CH.sub.2OH, CH(OH)CH.sub.3, CH.sub.2CH.sub.2CH.sub.2OH, CH(CH.sub.3)CH.sub.2OH, C(OH)(CH.sub.3).sub.2, CH.sub.2OCH.sub.3, CH.sub.2OCH.sub.2CH.sub.3, O—CH.sub.3, O—CH.sub.2CH.sub.3, and O—CF.sub.3.

[0095] According to another embodiment, R.sup.1 is selected from the group R.sup.1-G4 consisting of

H, F, Cl, Br, CH.sub.3, CH.sub.2CH.sub.3, CHF.sub.2, CN, CH.sub.2OH, and CH.sub.2OCH.sub.3.

[0096] According to another embodiment, R.sup.1 is selected from the group R.sup.1-G5 consisting of H.

[0097] According to another embodiment, R.sup.1 is selected from the group R.sup.1-G6 consisting of F, Cl, and Br.

[0098] According to another embodiment, R.sup.1 is selected from the group R.sup.1-G7 consisting of CH.sub.3.

[0099] According to another embodiment, R.sup.1 is selected from the group R.sup.1-G8 consisting of CH.sub.2CH.sub.3.

[0100] According to another embodiment, R.sup.1 is selected from the group R.sup.1-G9 consisting of

CH.sub.2CH.sub.2CH.sub.3, CH.sub.2CH(CH.sub.3).sub.2, cyclopropyl, and cyclobutyl.

[0101] According to another embodiment, R.sup.1 is selected from the group R.sup.1-G10 consisting of CHF.sub.2, and CF.sub.3.

[0102] According to another embodiment, R.sup.1 is selected from the group R.sup.1-G11 consisting of CN.

[0103] According to another embodiment, R.sup.1 is selected from the group R.sup.1-G12 consisting of

1-cyanocycloprop-1-yl, CH.sub.2CN, and C(CH.sub.3).sub.2CN.

[0104] According to another embodiment, R.sup.1 is selected from the group R.sup.1-G13 consisting of CH.sub.2OH.

[0105] According to another embodiment, R.sup.1 is selected from the group R.sup.1-G14 consisting of

CH.sub.2CH.sub.2OH, CH(OH)CH.sub.3, CH.sub.2CH.sub.2CH.sub.2OH, CH(CH.sub.3)CH.sub.2OH, and C(OH)(CH.sub.3).sub.2.

[0106] According to another embodiment, R.sup.1 is selected from the group R.sup.1-G15 consisting of

CH.sub.2OCH.sub.3, CH.sub.2OCH.sub.2CH.sub.3, O—CH.sub.3, O—CH.sub.2CH.sub.3, and O—CF.sub.3.

R.SUP.3.:

[0107] According to one embodiment, R.sup.3 is selected from the group R.sup.3-G1 consisting of

F, Cl, Br, CN, C.sub.1-4-alkyl optionally substituted with 1 to 5 F, C.sub.3-4-cycloalkyl, HO—C.sub.1-3-alkylene, C.sub.1-3-alkyl-O—C.sub.1-3-alkylene, and O—C.sub.1-4-alkyl optionally substituted with 1 to 5 F.

[0108] According to another embodiment, R.sup.3 is selected from the group R.sup.3-G2 consisting of

F, Cl, Br, CN, C.sub.1-3-alkyl optionally substituted with 1 to 3 F, HO—C.sub.1-4-alkylene, C.sub.1-2-alkyl-O—C.sub.1-2-alkylene, and O—C.sub.1-2-alkyl optionally substituted with 1 to 3 F.

[0109] According to another embodiment, R.sup.3 is selected from the group R.sup.3-G3 consisting of

Cl, CN, CH.sub.3, CF.sub.3, CH.sub.2CH.sub.3, CH(CH.sub.3).sub.2, CH.sub.2OH, CH.sub.2CH.sub.2OH, C(CH.sub.3).sub.2OH, and CH.sub.2OCH.sub.3.

[0110] According to another embodiment, R.sup.3 is selected from the group R.sup.3-G4 consisting of Cl and CN.

[0111] According to another embodiment, R.sup.3 is selected from the group R.sup.3-G5 consisting of CH.sub.3, CF.sub.3, CH.sub.2CH.sub.3, and CH(CH.sub.3).sub.2.

[0112] According to another embodiment, R.sup.3 is selected from the group R.sup.3-G6 consisting of

CH.sub.2OH, CH.sub.2CH.sub.2OH, and C(CH.sub.3).sub.2OH.

[0113] According to another embodiment, R.sup.3 is selected from the group R.sup.3-G7 consisting of CH.sub.2OCH.sub.3.

Stereochemistry:

[0114] According to one embodiment, the stereochemistry of the compound of formula (I) is according to formula (I.1)

##STR00032##

[0115] According to another embodiment, the stereochemistry of the compound of formula (I) is according to formula (I.2)

##STR00033##

[0116] Further preferred subgeneric embodiments of the compounds of formula (I) are set forth as embodiments (I-a) to (I-r) in the following Table 1, wherein the above-mentioned substituent definitions are used. For example, the entry -G1 in column R.sup.1 and row (I-a) means that in embodiment (I-a) substituent R.sup.1 is selected from the definition designated R.sup.1-G1. The same applies analogously to the other variables incorporated in the general formulas.

TABLE-US-00001 TABLE 1 Substituents Embodiment Y R Ar R.sup.1 R.sup.3 (I-a) Y-G1 R-G1 Ar-G1 R.sup.1-G1 R.sup.3-G1 (I-b) Y-G2 R-G2 Ar-G1 R.sup.1-G1 R.sup.3-G2 (I-c) Y-G2 R-G2 Ar-G2 R.sup.1-G2 R.sup.3-G2 (I-d) Y-G2 R-G3 Ar-G2 R.sup.1-G3 R.sup.3-G2 (I-e) Y-G2 R-G4 Ar-G3 R.sup.1-G3 R.sup.3-G3 (I-f) Y-G6 R-G5 Ar-G5 R.sup.1-G4 R.sup.3-G5 (I-g) Y-G6 R-G5 Ar-G6 R.sup.1-G4 R.sup.3-G5 (I-h) Y-G6 R-G5 Ar-G8 R.sup.1-G4 R.sup.3-G5 (I-i) Y-G6 R-G6 Ar-G5 R.sup.1-G4 R.sup.3-G5 (I-j) Y-G6 R-G6 Ar-G6 R.sup.1-G4 R.sup.3-G5 (I-k) Y-G6 R-G6 Ar-G8 R.sup.1-G4 R.sup.3-G5 (I-m) Y-G7 R-G5 Ar-G5 R.sup.1-G4 R.sup.3-G5 (I-n) Y-G7 R-G5 Ar-G6 R.sup.1-G4 R.sup.3-G5 (I-o) Y-G7 R-G5 Ar-G8 R.sup.1-G4 R.sup.3-G5 (I-p) Y-G7 R-G6 Ar-G5 R.sup.1-G4 R.sup.3-G5 (I-q) Y-G7 R-G6 Ar-G6 R.sup.1-G4 R.sup.3-G5 (I-r) Y-G7 R-G6 Ar-G8 R.sup.1-G4 R.sup.3-G5

[0117] Particularly preferred are those subgeneric embodiments (I.1-a) to (I.1-r) which, in respect of the definitions of Y, R, Ar, R.sup.1, and R.sup.3 correspond to the subgeneric embodiments (I-a) to (I-r) of Table 1, but wherein the stereochemistry of the compounds is according to formula (I.1).

[0118] According to another preferred embodiment, the stereochemistry of the compounds of the present invention is according to formula (I.1) wherein Ar is selected from the group Ar-G5.

[0119] According to another preferred embodiment, the stereochemistry of the compounds of the present invention is according to formula (I.1) wherein Ar is selected from the group Ar-G6.

[0120] According to another preferred embodiment, the stereochemistry of the compounds of the present invention is according to formula (I.1) wherein Ar is selected from the group Ar-G8.

[0121] Particularly preferred compounds, including their tautomers, the salts thereof, or any solvates, hydrates or cocrystals thereof, are those described in the section Examples and Experimental Data.

Preparation

[0122] The compounds according to the invention and their intermediates may be obtained using methods of synthesis which are known to the one skilled in the art and described in the literature of organic synthesis for example using methods described in “Comprehensive Organic Transformations”, 2.sup.nd Edition, Richard C. Larock, John Wiley & Sons, 2010, and “March's Advanced Organic Chemistry”, 7.sup.th Edition, Michael B. Smith, John Wiley & Sons, 2013. Preferably the compounds are obtained analogously to the methods of preparation explained more fully hereinafter, in particular as described in the experimental section. In some cases the sequence adopted in carrying out the reaction schemes may be varied. Variants of these reactions that are known to the skilled person but are not described in detail here may also be used. The general processes for preparing the compounds according to the invention will become apparent to the skilled person on studying the schemes that follow. Starting compounds are commercially available or may be prepared by methods that are described in the literature or herein, or may be prepared in an analogous or similar manner. Before the reaction is carried out, any corresponding functional groups in the starting compounds may be protected using conventional protecting groups. These protecting groups may be cleaved again at a suitable stage within the reaction sequence using methods familiar to the skilled person and described in the literature for example in “Protecting Groups”, 3.sup.rd Edition, Philip J. Kocienski, Thieme, 2005, and “Protective Groups in Organic Synthesis”, 4.sup.th Edition, Peter G. M. Wuts, Theodora W. Greene, John Wiley & Sons, 2006.

##STR00034##

[0123] Scheme 1: Compounds of formula (I′) can be prepared by reacting a suitable acid of formula (II) (either as free acid or carboxylate with a suitable metal cation such as Li.sup.+, Na.sup.+, K.sup.+, etc.) and a suitable amine of formula (III) (either as free amine or a salt such as hydrochloride, hydrobromide, etc.) in a suitable solvent (e.g., DCM, THF, 1,4-dioxane, DMF, N,N-dimethylacetamide, and 1-methyl-2-pyrrolidinone) in the presence of a suitable coupling agent (e.g., O-(7-azabenzotriazol-1-yl)-N,N,N′,N′-tetramethyluronium-hexafluorophosphate (HATU), O-(benzotriazol-1-yl)-N,N,N′,N′-tetramethyluronium tetrafluoroborate (TBTU), (benzotriazol-1-yloxy)tripyrrolidinophosphonium hexafluorophosphate (PyBOP), carbodiimide reagents, etc.) and a base (e.g., triethylamine, N,N-diisopropyl-ethylamine, pyridine, etc.) to form an amide bond; Y, R, and Ar in Scheme 1 have the meanings as defined hereinbefore. Alternatively, the carboxylic acid is transformed into a carboxylic chloride (using, e.g., oxalyl chloride or thionyl chloride in DCM) and coupled as such with amine (III) in the presence of a suited base (e.g., triethylamine, N,N-diisopropyl-ethylamine, pyridine, etc.).

##STR00035##

[0124] Scheme 2: Acids of formula (II), wherein Y, R, and Ar have the meanings as defined hereinbefore, are preferably prepared from the corresponding ester (IV) through hydrolysis or hydrogenolysis depending on the nature of R.sup.5. Lower alkyl group esters such as ethyl or methyl esters are preferably cleaved by hydrolysis with a hydroxide salt such as NaOH, LiOH, or KOH in a mixture of water and a suitable miscible solvent (e.g., THF, MeOH, EtOH, 1,4-dioxane, or mixtures of these) at ambient or elevated temperature. The acid may be isolated either as a salt with the metal cation or as free acid. A tert-butyl ester is preferably cleaved by treatment with an acid (e.g., hydrochloric acid or TFA) in a suitable solvent (e.g., DCM, 1,4-dioxane, MeOH, EtOH, THF, water, or mixtures of these). A benzyl ester is preferably cleaved by hydrogenolysis with a suitable catalyst (e.g., palladium on carbon) in a suitable solvent (e.g., EtOH, MeOH, THF, DCM, or EtOAc) under an atmosphere of hydrogen (preferably 1 to 5 bar).

##STR00036##

[0125] Scheme 3: Some of the compounds (II′) can be prepared by reaction of an alcohol (V) with an ester (VI) employing the conditions of the Mitsunobu reaction (e.g., triphenylphosphine or tri-n-butylphosphine combined with, e.g., diethyl azodicarboxylate (DEAD), diisopropyl azodicarboxylate (DIAD), or di-tert-butyl azodicarboxylate (DBAD) in a solvent such as THF, 1,4-dioxane, toluene, etc.); Y, R, and R.sup.3 in Scheme 3 have the meanings as defined hereinbefore. Alcohol (V) may bear the desired residue R on the heteroaromatic ring Y or a leaving group instead to introduce R later on. Alternatively, some of the compounds (II′) can be obtained by reacting alcohol (V) and ester (VI) in the presence of a Lewis acid or Brønsted acid (e.g., 4-toluenesulfonic acid) in a suited solvent (e.g., MeCN) at elevated temperature (20 to 120° C.).

##STR00037##

[0126] Scheme 4: Some of the compounds (II′) can also be prepared by reaction of compound (VII), bearing a leaving group at the heteroarylmethyl position such as Cl, Br, or mesyloxy (methanesulfonyloxy), with ester (VI) in the presence of a suitable base (e.g., sodium hydride, cesium carbonate, potassium carbonate, or triethylamine) in a suitable solvent (e.g., THF, DMF); Y, R, and R.sup.3 in Scheme 4 have the meanings as defined hereinbefore. Compound (VII) may bear the desired residue R on the heteroaromatic ring Y or a leaving group instead to introduce R later on.

##STR00038##

[0127] Scheme 5: Some esters of formula (II″), wherein Y and R have the meanings defined hereinbefore, can be prepared by treatment of a corresponding alkyl halide (bromide or chloride) or sulfonate (e.g., mesylate) of formula (VII) with sodium azide in DMF or another suitable solvent to give an intermediate of formula (VIII) which is then reacted with a suitable propiolic acid ester under copper mediated conditions (e.g., ethyl propiolate or tert-butyl propiolate with catalytic copper sulfate and sodium ascorbate in water/tert-butanol) to give compound (II″). Alternatively, azide (VIII) can be obtained from an alcohol of formula (V) (or (VII) wherein Hal is OH) by treatment with diphenylphosphoryl azide in the presence of a suitable base such as DBU in a suitable solvent (e.g., THF or DMF). Compound (VII) may bear the desired residue R on the heteroaromatic ring Y or a leaving group instead to introduce R later on.

##STR00039##

[0128] Scheme 6: Esters of formula (II′″), wherein Y, R, and R.sup.3 have the meanings defined hereinbefore, can be prepared from alcohols (XII) by displacement of the hydroxyl group with hydrogen employing well known methods reported in the literature (e.g., triethylsilane and TFA or borontrifluoride etherate in DCM, or hydrogen in the presence of palladium on carbon in a solvent such as THF or EtOH). Alcohols (XII) may be prepared by adding magnesium halide (XI), or another organometal derivative, to aldehyde (IX), that, in turn, can be obtained from its corresponding alcohol by oxidation (e.g., Dess-Martin oxidation or Swern oxidation) in an inert solvent (e.g., THF, DCM, or diethyl ether) at low to ambient temperature. Magnesium halide (XI) may be obtained after a halogen metal exchange reaction from the corresponding bromide or iodide of (X) using isopropyl magnesium chloride optionally combined with lithium chloride in THF at low temperature. Alternatively, magnesium metal is inserted into the carbon halogen bond to provide magnesium halide (XI). Compounds (IX) and (XII) may bear the desired residue R on the heteroaromatic ring Y or a leaving group instead to introduce R later on.

[0129] Starting from the compounds (X′) and (X″) the corresponding analogs of compound (II′″) are accessible employing the principle proceeding delineated above (see Scheme 7 for (X′)).

##STR00040##

[0130] Scheme 7: Compounds of formula (II″″), wherein Y, R, and R.sup.3 have the meanings defined hereinbefore, can be prepared in an analogous fashion to the compounds delineated in Scheme 6 using the isomeric magnesium halide (XI′).

##STR00041##

[0131] Scheme 8: Intermediates of formula (XV) can be prepared from aromatic compound (XIII) and amine (XIV) via either a nucleophilic substitution reaction on the heteroaromatic ring or a transition metal catalyzed coupling reaction; Ar, R and R.sup.1 in Scheme 8 have the meanings defined hereinbefore. The nucleophilic substitution of a leaving group on the heteroaromatic ring in (XIII) with the N in compound (XIV) can be conducted in the presence of a suitable base (e.g., sodium hydride, cesium carbonate, potassium carbonate, N,N-diisopropyl-ethylamine) in a suitable solvent (e.g., THF, 1,4-dioxane, DMF, DMSO) at ambient or elevated temperature. A transition metal catalyzed coupling reaction is preferably carried out in analogy to procedures reported in the literature of organic chemistry referred to as Ullmann or Buchwald/Hartwig coupling reaction using suitable copper or palladium salts or complexes thereof, optionally combined with additional ligands, in the presence of a base and in a suited solvent.

##STR00042##

[0132] Scheme 9: Enantiopure amine (III.1) can be prepared from ketone (XVI) as delineated in Scheme 9. The overall synthesis comprises 3 steps and starts with the condensation of the ketone with the enantiopure tert-butanesulfinamide in the presence of a dehydrating agent such as titanium alcoholate (e.g., Ti(OEt).sub.4 or Ti(O.sup.iPr).sub.4) in a suited solvent (e.g., THF, DCM, toluene, or neat) at ambient or elevated temperature to generate the corresponding enantiopure tert-butylsulfinylated imine (XVII). Imine (XVII) can be diastereoselectively reduced to the corresponding tert-butylsulfinylated amine (XVIII) using a hydride (e.g., lithium or sodium borohydride, L-selectride, diisobutylaluminum hydride, etc.) in a suited solvent (e.g., THF, toluene, MeOH, etc., depending on the hydride source used). The tert-butylsulfinyl group can be cleaved off using an acid (e.g., TFA or hydrochloric acid) in a suitable solvent (e.g., toluene, DCM, dioxane, alcohol, water, etc.) at ambient or elevated temperature.

[0133] The racemate (III) and the opposite enantiomer (III.2) are obtained by employing the racemic tert-butanesulfinamine and enantiopure (S)-tert-butanesulfinamine, respectively, in the route described above.

[0134] Alternatively, compound (III.1) and its enantiomer (III.2) can be obtained from ketone (XVI) via a 3-step synthesis sequence starting with an enantioselective reduction of the ketone moiety in compound (XVI) using conditions reported in the literature of organic chemistry (e.g., J. Am. Chem. Soc. 1995, 117, 7562-3; Org. Lett. 2010, 12, 1756-9; Org. Proc. Res. Dev. 2006, 10, 949-958; Tetrahedron: Asymmetry 2003, 14, 2659-2681; Tetrahedron Lett. 2014, 55, 3635-40; and references quoted therein) to give the corresponding enantiopure or enantioenriched alcohol. The thus formed hydroxyl group can then be replaced with a protected or masked ammonia group such as phthalimide or (tert-Bu-OCO).sub.2N employing a stereospecific Mitsunobu or Mitsunobu-type reaction (using, e.g., triphenylphosphine or tri-n-butylphosphine combined with dimethyl azodicarboxylate, DEAD, DIAD, di-(4-chlorobenzyl) azodicarboxylate, dibenzyl azodicarboxylate, DBAD, azodicarboxylic acid bis-(dimethylamide), azodicarboxylic acid dipiperidide, or azodicarboxylic acid dimorpholide in a suitable solvent (e.g., THF, 1,4-dioxane, EtOAc, benzene, toluene, etc.)) leading to inversion of the configuration at the heteroatom bearing C. Alternatively, a phosphoryl azide (e.g., diphenylphosphoryl azide) can be employed to replace the OH group with azide under inversion of the configuration. The amino group can be liberated from the phthalimide group by treatment with, e.g., hydrazine, hydroxylamine, methylamine, n-butylamine, or ethanolamine in a suitable solvent (e.g., EtOH, MeOH, MeCN, THF, dioxane, DMSO, N,N-dimethylacetamide, water, or mixtures of these) with heating if necessary to give compound (III.1). tert-Bu-O—CO is preferably removed under acidic conditions (using, e.g., TFA or hydrochloric acid) to give the same amine (III.1). The azide can be reduced to the amine (III.1) with, e.g., hydrogen in the presence of a transition metal (e.g., Pd on carbon, Raney-Ni, PtO.sub.2, etc.) or a phosphine (e.g., triphenylphosphine). The racemate (III) is obtained upon reduction of compound (XVI) with an achiral reducing agent such as sodium borohydride and following the further route described above.

##STR00043##

[0135] Scheme 10: Compounds (XVI) can be obtained from ester (XIX) (or the corresponding lower or higher alkyl esters, e.g., methyl, n-propyl, isopropyl, or tert-butyl ester) in a sequence consisting of 3 or 4 reaction steps. Compound (XIX) can be brominated employing a suited electrophilic bromine source (e.g., N-bromosuccinimide (NBS) or Br.sub.2) in a suited solvent (e.g., AcOH, DCM, dichloroethane, dioxane, MeCN, DMF, etc.) at ambient or elevated temperature. For example, NBS in acetic acid at ambient temperature or NBS in HCCl.sub.3 at 65° C. provide the compound. Alternatively, treatment of compound (XIX) with NBS, sodium persulfate and palladium acetate in trifluoromethanesulfonic acid and 1,2-dichloroethane at 80° C. gives access to (XX) as well. Compound (XX) can then be transformed into ester (XXI) by applying a 1- or 2-step synthesis route encompassing a Fleck coupling reaction (broadly covered in the literature of organic chemistry, e.g., in Catalysts 2017, 7, 267 and references quoted therein) with either acrolein dialkyl acetal (e.g., acrolein diethyl acetal, ->(XXI)) or an acrylic acid ester (e.g., acrylic ethyl ester, ->(XXIII)); using the latter coupling partner requires an additional step to reduce the olefinic bond formed routinely achieved with hydrogen in the presence of a transition metal catalyst (e.g., Pd such as palladium on carbon, Ni such as Raney-Ni, Pt such as platinum oxide, Rh such as rhodium on carbon, etc.) in a suited solvent (e.g., DCM, dioxane, THF, EtOAc, alcohol such as MeOH, water, etc.). Ketoester (XXII) may be produced upon treatment of compound (XXI) with a base (e.g., a hydride such as sodium hydride, an alcoholate such as lithium methoxide or potassium tert-butylate, an organic amine such as DBU, a phosphazene such as P.sub.2Et phosphazene, an amide such as lithium diisopropylamide, lithium, sodium or potassium hexamethyldisilazide, etc.) in a suited solvent (e.g., benzene, toluene, dioxane, THF, alcohol, etc., depending on the base used) at low to elevated temperature (−78° C. to 100° C., depending on the base and solvent employed); potassium hexamethyldisilazide in THF at 20° C. is one of the more preferred conditions for this transformation. Hydrolysis of the ester group in compound (XXII) followed by decarboxylation can be achieved by stirring the compound in a solvent (e.g., dioxane, THF, ACN, DMF, N,N-dimethylacetamide, DMSO, alcohol, water, etc., or mixtures of these), optionally in the presence of a base (e.g., sodium hydroxide), a halide salt such as lithium iodide or chloride, or an acid (e.g., hydrochloric acid) at 0 to 140° C. to give ketone (XVI).

[0136] The compounds of formula (I) may be resolved into their enantiomers and/or diastereomers as mentioned below. Thus, for example, cis/trans mixtures may be resolved into their c/'s and trans isomers and racemic compounds may be separated into their enantiomers.

[0137] The cis/trans mixtures may be resolved, for example, by chromatography into the c/'s and trans isomers thereof. The compounds of formula (I) which occur as racemates may be separated by methods known per se into their optical antipodes and diastereomeric mixtures of compounds of general formula (I) may be resolved into their diastereomers by taking advantage of their different physico-chemical properties using methods known per se, e.g. chromatography and/or fractional crystallization; if the compounds obtained thereafter are racemates, they may be resolved into the enantiomers as mentioned below.

[0138] The racemates are preferably resolved by column chromatography on chiral phases or by crystallization from an optically active solvent or by reacting with an optically active substance which forms salts or derivatives such as esters or amides with the racemic compound. Salts may be formed with enantiomerically pure acids for basic compounds and with enantiomerically pure bases for acidic compounds. Diastereomeric derivatives are formed with enantiomerically pure auxiliary compounds, e.g. acids, their activated derivatives, or alcohols. Separation of the diastereomeric mixture of salts or derivatives thus obtained may be achieved by taking advantage of their different physico-chemical properties, e.g. differences in solubility; the free antipodes may be released from the pure diastereomeric salts or derivatives by the action of suitable agents. Optically active acids commonly used for such a purpose as well as optically active alcohols applicable as auxiliary residues are known to those skilled in the art.

[0139] As mentioned above, the compounds of formula (I) may be converted into salts, particularly for pharmaceutical use into the pharmaceutically acceptable salts. As used herein, “pharmaceutically acceptable salts” refer to derivatives of the disclosed compounds wherein the parent compound is modified by making acid or base salts thereof.

[0140] The compounds according to the invention are advantageously also obtainable using the methods described in the examples that follow, which may also be combined for this purpose with methods known to the skilled person from the literature.

[0141] Thus, according to another aspect of the present invention, processes for the synthesis of compounds of formula (I) are provided.

[0142] According to another aspect of the present invention, intermediates of the synthesis of compounds of formula (I) are provided.

[0143] According to one embodiment, the invention relates to intermediates as depicted and described in Schemes 9 and/or 10.

[0144] According to another embodiment, the invention relates to one or more of the following intermediates

##STR00044##

Pharmacological Activity

[0145] The activity of the compounds of the invention may be demonstrated using the following assays:

Biological Methods

[0146] The ability of compounds of formula (I) to inhibit plasma kallikrein (PKK), Factor XIIa (FXIIa), Factor XIa (FXIa), Factor Xa (FXa), Factor IIa (alpha-thrombin; FIIa), plasmin, trypsin, tissue kallikrein 1 (TK1), Factor Vila (FVIIa), or FVIIa complexed with Tissue Factor, phospholipids and CaCl.sub.2 (FVIIa/TF/PL/CaCl.sub.2) is determined using the following biochemical assays in assay buffer (100 mM Tris, 150 mM NaCL, adjusted to a pH of 7.8 with HCl, and containing 0.1% (w/v) BSA and 0.05% (v/v) Tween20) in the presence of 1% (v/v) DMSO:

Evaluation of the Inhibition of PKK Using an Endpoint Assay

[0147] Human PKK (0.01 U/mL; Enzyme Research Laboratories) or rat PKK (0.625 nM; produced in-house) is incubated for 1 h at room temperature with 0.10 μM fluorogenic substrate H-Pro-Phe-Arg-AMC (11295 from Bachem) and various concentrations of the test compound in assay buffer. Subsequently, PPACK II (Calbiochem) is added as a stop solution to achieve a final concentration of 1 μM and fluorescence is measured using an Envision Reader (PerkinElmer) with the wavelength excitation setting of 355 nm and the wavelength emission setting of 460 nm.

[0148] IC.sub.50 values for compounds according to the invention are shown in the following table. The number of the compound corresponds to the number of the Example in the experimental section.

TABLE-US-00002 Example IC.sub.50 (nM) 1 1.3 2 3.1 3 1.7 4 0.9 5 1.1 6 0.9 7 1.4 8 2.3 9 3.8 10 2.5 11 2.1 12 3.4 13 1.1 14 1.0 15 1.1 16 0.6 17 2.0 18 2.1 19 14.2 20 0.7 21 0.9 22 0.5 23 1.1 24 1.0 25 2.0 26 4.9 27 0.9 28 0.6 29 2.6 30 0.9 31 5.3 32 1.1 33 2.5 34 0.9 35 2.2 36 0.5 37 0.9 38 3.4 39 0.7 40 1.2 41 3.4 42 0.6 43 0.9 44 1.8 45 0.7 46 1.0 47 0.8 48 2.8 49 2.5 50 1.7 51 1.0 52 1.1 53 2.0 54 0.7 55 2.3 56 1.8 57 0.6 58 2.5 59 0.4 60 18.8 61 3.1 62 14.0 63 3.0 64 0.7 65 1.4 66 0.7 67 3.1 68 1.4 69 0.9 70 0.8 71 151 72 3.3 73 9.6 74 6.9 75 0.5 76 0.6 77 3.3 78 6.8 79 5.5 80 4.7 81 1.3 82 0.8 83 1.7 84 0.7 85 0.8 86 1.2 87 3.3 88 0.9 89 77.5 90 0.7 91 2.1 92 2.8 93 1.4 94 10.8 95 5.5 96 1.5 97 1.4 98 1.5 99 3.7 100 5.6 101 0.6 102 2.1 103 0.7 104 3.2 105 0.5 106 9.8 107 1.0 108 1.7 109 0.8 110 0.9 111 8.6 112 12.6 113 9.9 114 1.8 115 4.1 116 0.4 117 2.2 118 0.3 119 1.2 120 2.4 121 1.6 122 1.5 123 0.9 124 2.4 125 1.1 126 0.6 127 1.2 128 5.1 129 23.7 130 20.0 131 4.3 132 1.9 133 1.6 134 1.7 135 4.2 136 2.0 137 4.4 138 2.2 139 9.6 140 5.8 141 2.0 142 5.8 143 0.6 144 0.8 145 0.7 146 1.1 147 1.4 148 1.4 149 1.6 150 1.0 151 3.6 152 1.2 153 0.5 154 9.9 155 15.4 156 0.8 157 1.2 158 4.6 159 3.5 160 2.2 161 1.5 162 1.1 163 2.2 164 1.8 165 0.8 166 1.0 167 1.3 168 1.5 169 0.6 170 0.8 171 2.6 172 8.1 173 0.6 174 1.9 175 2.9 176 4.2 177 5.5 178 5.0 179 4.2 180 2.6 181 10.9 182 7.1 183 19.4 184 13.5 185 10.9 186 10.9 187 12.2 188 19.8 189 22.1 190 1.5 191 169 192 1890 193 0.4 194 0.3 195 20.3 196 1.5 197 13.4 198 0.8 199 3.1 200 8.6 201 0.6 202 0.5 203 0.7 204 1.5 205 8.3 206 0.4 207 3.2 208 0.6 209 1.5 210 0.4 211 0.8 212 2.1 213 2.3 214 20.3 215 0.7 216 1.2 217 0.9 218 3.5 219 3.6 220 1.9 221 2.8 222 11.6 223 7540 224 302 225 232 226 35.7 227 45.3 228 126 229 110 230 53.7 231 223 232 512 233 81.4 234 350 235 214 236 222 237 111 238 123 239 123 240 52

Evaluation of the Inhibition of PKK in Kaolin Activated Human PPP

[0149] Platelet poor plasma (PPP) obtained from human wholeblood, anticoagulated with Na-Citrate, is incubated with various concentrations of the test compound together with either 25, 75, 250, or 750 pig/mL kaolin in assay buffer for 20 min at 37° C. such that for each kaolin dose used a concentration response is obtained for the test compound. Afterwards 0.25 mM fluorogenic substrate H-Pro-Phe-Arg-AMC (11295 from Bachem) is added to the mixture and measurements are performed in a kinetic interval every 2nd minute for 12 min using a Spectramax M5 (Molecular Devices) with the following settings of the wavelength excitation of 350 nm and wavelength emission of 450 nm. pIC50 and pIC90 values are obtained from 4 x/y-plots (x=log M, Compound; y=delta rfu/min) fitted with GraphPad prism 7.0 (Equation: log(agonist) vs. response—Find EC anything; the four concentration response curves obtained for the test compound, each obtained using a different kaolin dose, are fitted using a global fitting procedure yielding shared pIC50 or pIC90 values).

[0150] IC.sub.90 values for compounds according to the invention are shown in the following table. The number of the compound corresponds to the number of the Example in the experimental section.

TABLE-US-00003 Example IC.sub.90 (nM) 1 290 2 253 3 210 4 184 5 278 6 1880 7 151 8 352 9 603 10 789 11 300 12 802 13 451 14 636 15 414 16 280 17 818 18 460 20 99 21 325 22 249 23 233 24 181 25 286 26 343 27 202 28 184 29 336 30 334 31 973 32 548 33 265 34 1190 35 259 36 180 37 125 39 380 40 543 41 488 42 195 43 308 44 444 45 151 46 162 47 173 48 612 49 254 50 110 51 213 52 155 53 203 54 109 55 250 56 586 57 166 62 769 63 203 64 221 65 1230 66 839 67 635 68 637 69 328 70 797 71 9310 72 831 73 498 74 321 75 192 76 270 77 197 78 317 79 249 80 495 81 117 82 117 83 594 84 209 85 132 86 597 87 177 88 139 89 3430 90 126 91 439 92 307 93 110 94 1760 95 577 96 134 97 766 98 282 99 2170 101 297 102 1440 103 531 104 706 105 117 106 1970 107 324 108 465 109 414 110 784 111 4150 112 2780 114 14700 115 2660 116 278 117 1540 118 157 119 1460 120 934 121 514 122 492 123 1320 124 823 125 2740 126 681 127 1680 128 1390 131 962 132 425 133 560 134 1030 135 909 136 310 137 756 139 774 140 760 141 621 142 731 143 1170 144 248 145 245 146 274 147 149 148 985 149 511 150 373 151 1140 152 430 153 280 154 4270 155 4100 158 2100 159 1140 160 967 161 431 162 231 163 470 164 443 165 342 166 193 167 272 168 1860 169 485 170 469 171 268 181 22300 182 6270 184 2310 185 2270 186 236 187 2450 188 1500 190 1080 193 253 194 183 196 350 198 398 199 808 201 130 202 157 203 202 204 818 206 138 207 745 208 80 173 729 174 1430 175 822 177 1180 178 844 179 389 180 207 209 432 210 128 211 590 212 1890 213 621 214 1040 215 194 216 >10000 217 624 218 646 219 776 220 525

Evaluation of the Inhibition of PKK (K.SUB.i.)

[0151] Human PKK (1.78 nM or 0.025 U/mL; Enzyme Research Laboratories) is incubated at 24° C. with 0.25 mM fluorogenic substrate H-Pro-Phe-Arg-AMC (11295 from Bachem) and various concentrations of the test compound in assay buffer. Measurements are performed in a kinetic interval every 2.sup.nd minute for 16 min using a Spectramax M5 (Molecular Devices) with the following settings of the wavelength excitation of 350 nm and wavelength emission of 450 nm.

Evaluation of the Inhibition of FXIIa (K.SUB.i.)

[0152] Human FXIIa (47.5 nM or 1.1 U/mL; Enzyme Research Laboratories) is incubated at 24° C. with 0.5 mM chromogenic Substrate S2302 (Chromogenix) and various concentrations of the test compound in assay buffer. Measurements are performed in a kinetic interval every 2.sup.nd minute for 16 min using a Spectramax M5 (Molecular Devices) measuring the optical absorbance at 405 nm.

Evaluation of the Inhibition of FXIa (K.SUB.i.)

[0153] Human FXIa (0.5 nM or 0.016 U/mL; Enzyme Research Laboratories) is incubated at 24° C. with 0.25 mM fluorogenic substrate Boc-Glu(OBzl)-Ala-Arg-AMC.HCl (I1575 from Bachem) and various concentrations of the test compound in assay buffer. Measurements are performed in a kinetic interval every 2.sup.nd minute for 16 min using a Spectramax M5 (Molecular Devices) with the following settings of the wavelength excitation of 350 nm and wavelength emission of 450 nm.

Evaluation of the Inhibition of FXa (K.SUB.i.)

[0154] Human FXa (0.86 nM or 0.01 U/mL; Enzyme Research Laboratories) is incubated at 24° C. with 0.5 mM chromogenic Substrate S2765 (Chromogenix) and various concentrations of the test compound in assay buffer. Measurements are performed in a kinetic interval every 2.sup.nd minute for 16 min using a Spectramax M5 (Molecular Devices) measuring the optical absorbance at 405 nm.

Evaluation of the Inhibition of FIIa (K.SUB.i.)

[0155] Human FIIa (44.6 nM or 5 U/mL; Enzyme Research Laboratories) is incubated at 24° C. with 0.5 mM chromogenic Substrate S2238 (Chromogenix) and various concentrations of the test compound in assay buffer. Measurements are performed in a kinetic interval every 2.sup.nd minute for 16 min using a Spectramax M5 (Molecular Devices) measuring the optical absorbance at 405 nm.

Evaluation of the Inhibition of Plasmin (K.SUB.i.)

[0156] Human plasmin (64.1 nM or 0.0275 U/mL; Enzyme Research Laboratories) is incubated at 24° C. with 0.3 mM chromogenic Substrate S2251 (Chromogenix) and various concentrations of the test compound in assay buffer. Measurements are performed in a kinetic interval every 2.sup.nd minute for 16 min using a Spectramax M5 (Molecular Devices) measuring the optical absorbance at 405 nm.

Evaluation of the Inhibition of Trypsin (K.SUB.i.)

[0157] Human trypsin (4.54 nM or 250 U/mL; Calbiochem) is incubated at 24° C. with 0.5 mM chromogenic Substrate S2222 (Chromogenix) and various concentrations of the test compound in assay buffer. Measurements are performed in a kinetic interval every 2.sup.nd minute for 16 min using a Spectramax M5 (Molecular Devices) measuring the optical absorbance at 405 nm.

Evaluation of the Inhibition of Tk1 (K.SUB.i.)

[0158] Prior to the assay, human TK1 (R&D Systems) is activated by incubation with human trypsin (Calbiochem) in a 1:10,000 ratio for 15 min at 37° C. For assaying TK1 inhibitory activity, activated TK1 (31.25 nM or 1 U/mL) is incubated at 24° C. with 0.1 mM fluorogenic substrate H-Pro-Phe-Arg-AMC (11295 from Bachem) and various concentrations of the test compound in assay buffer. Measurements are performed in a kinetic interval every 2.sup.nd minute for 16 min using a Spectramax M5 (Molecular Devices) with the following settings of the wavelength excitation of 350 nm and wavelength emission of 450 nm.

[0159] K.sub.i values for compounds according to the invention are shown in the following table. The number of the compound corresponds to the number of the Example in the experimental section.

TABLE-US-00004 Example K.sub.i (nM) 4 >10000 16 >10000 20 >10000 22 >10000 23 >10000 24 >10000 27 >10000 36 >10000 47 >10000 49 >10000 51 >10000 52 >10000 75 >10000 80 >10000 86 >10000 90 >10000 107 >10000 108 >10000 110 >10000 113 >10000 114 >10000 115 >10000 116 871 117 >10000 118 >10000 119 1060 120 >10000 121 >10000 122 >10000 123 >10000 124 >10000 125 >10000 126 >10000 127 >10000 128 >10000 131 >10000 133 >10000 134 >10000 135 >10000 136 >10000 137 >10000 169 >10000 170 >10000 171 >10000 173 >10000 175 >10000 177 >10000 178 >10000 181 >10000 190 >10000 193 >10000 194 >10000 196 >10000 198 >10000 217 >10000 218 >10000 222 7280

Evaluation of the Inhibition of FVIIa (K.SUB.i.)

[0160] Human FVIIa (0.86 nM or 0.01 U/mL; Enzyme Research Laboratories) is incubated at 24° C. with 1.5 mM chromogenic Pefachrome® FVIIa (Loxo) and various concentrations of the test compound in assay buffer. Measurements are performed in a kinetic interval every 2.sup.nd minute for 16 min using a Spectramax M5 (Molecular Devices) measuring the optical absorbance at 405 nm.

Evaluation of the Inhibition of FVIIa/TF/PL/CaCl.sub.2 (K.sub.i)

[0161] Human FVIIa (300 nM or 585 U/mL; Enzyme Research Laboratories) together with 10 mM CaCl.sub.2*2H.sub.2O and 13.3% (v/v) Dade®Innovin® (Siemens; OQUMI94E0002(5534), which contains recombinant human tissue factor synthetic phospholipids (thromboplastin), is incubated at 24° C. with 1.5 mM chromogenic Pefachrome® FVIIa (Loxo) and various concentrations of the test compound in assay buffer. Measurements are performed in a kinetic interval every 2.sup.nd minute for 16 min using a Spectramax M5 (Molecular Devices) measuring the optical absorbance at 405 nm.

Calculation of pIC.sub.50 and pK.sub.i Values

[0162] The average V.sub.max values for the time interval from 2 to 12 min after initiation of the assay (expressed as either delta OD/min for assays using a chromogenic substrate or delta RFU/min for assays using a fluorigenic substrate, respectively) are plotted versus the Log of the concentration in molar of the evaluated inhibitor compound. The pIC.sub.50 values are then fitted using a four-parametric fitting procedure using GraphPad Prism (version 6; GraphPad Software, Inc.). Respective K.sub.i values are obtained by correction of the IC.sub.50 values for the respective KM value of the used substrate (see Table A for the obtained KM values of the used substrates) using the following formula:

[00001] $K_{i} = \frac{{IC}_{50}}{1 + \frac{[Substrate, mM]}{K_{M}}}$

Where the IC.sub.50 is in molar and the KM value in mM.

TABLE-US-00005 TABLE A K.sub.M values obtained for the substrates used in the enzymatic assays. Enzyme Substrate K.sub.M (mM) PKK I1295 0.16 FXIIa S2302 0.20 FXIa I1575 0.29 FXa S2765 1.31 FIIa S2238 1.25 Plasmin S2251 1.45 Trypsin S2222 2.03 TK1 I1295 0.07 FVIIa Pefachrome ® FVIIa 0.42 FVIIa/TF/PL/CaCl.sub.2 Pefachrome ® FVIIa 3.92

Evaluation of Permeability

[0163] Caco-2 cells (1-2×10.sup.5 cells/1 cm.sup.2 area) are seeded on filter inserts (Costar transwell polycarbonate or PET filters, 0.4 μm pore size) and cultured (DMEM) for 10 to 25 days.

[0164] Compounds are dissolved in appropriate solvent (like DMSO, 1-20 mM stock solutions). Stock solutions are diluted with HTP-4 buffer (128.13 mM NaCl, 5.36 mM KCl, 1 mM MgSO.sub.4, 1.8 mM CaCl.sub.2, 4.17 mM NaHCO.sub.3, 1.19 mM Na.sub.2HPO.sub.4×7H.sub.2O, 0.41 mM NaH.sub.2PO.sub.4×H.sub.2O, 15 mM HEPES, 20 mM glucose, pH 7.2) containing 0.25% BSA to prepare the transport solutions (0.1-300 μM compound, final DMSO<=0.5%). The transport solution (TL) is applied to the apical or basolateral donor side for measuring A-B or B-A permeability (2 filter replicates), respectively. The receiver side contains HTP-4 buffer supplemented with 0.25% BSA. Samples are collected at the start and end of experiment from the donor and at various time intervals for up to 2 hours also from the receiver side for concentration measurement by HPLC-MS/MS or scintillation counting. Sampled receiver volumes are replaced with fresh receiver solution.

Evaluation of Metabolic Stability in Human or Rat Liver Microsomes

[0165] The metabolic degradation of the test compound is assayed at 37° C. with pooled human (HLM) or rat liver microsomes (RLM). The final incubation volume of 60 μl per time point contains TRIS buffer pH 7.6 at RT (0.1 M), magnesium chloride (5 mM), microsomal protein (HLM: 1 mg/mL, RLM: 0.5 mg/mL) and the test compound at a final concentration of 1 μM.

[0166] Following a short preincubation period at 37° C., the reactions are initiated by addition of beta-nicotinamide adenine dinucleotide phosphate, reduced form (NADPH, 1 mM) and terminated by transferring an aliquot into solvent after different time points. Additionally, the NADPH-independent degradation is monitored in incubations without NADPH, terminated at the last time point. The quenched incubations are pelleted by centrifugation (10000 g, 5 min). An aliquot of the supernatant is assayed by LC-MS/MS for the amount of parent compound. The half-life (t1/2 INVITRO) is determined by the slope of the semilogarithmic plot of the concentration-time profile.

Evaluation of Metabolic Stability in Human or Rat Hepatocytes

[0167] The metabolic degradation of the test compound is assayed in a hepatocyte suspension. After recovery from cryopreservation, human or rat hepatocytes are incubated in Dulbecco's modified eagle medium supplemented with 3.5 μg glucagon/500 ml, 2.5 mg insulin/500 ml and 3.75 mg/500 ml hydrocortisone) containing 5% or 50% human or rat serum or in absence of serum.

[0168] Following a 30 min preincubation in a cell culture incubator (37° C., 10% CO.sub.2), test compound solution is spiked into the hepatocyte suspension to obtain a final cell density of 1.0*106 cells/ml, a final test compound concentration of 1 μM, and a final DMSO concentration of 0.05%.

[0169] The cells are incubated for six hours (incubator, horizontal shaker) and samples are removed from the incubation after 0, 0.5, 1, 2, 4 and 6 hours. Samples are quenched with acetonitrile and pelleted by centrifugation. The supernatant is transferred to a 96-deepwell plate, and prepared for analysis of decline of parent compound by HPLC-MS/MS.

[0170] CL.sub.int is calculated as follows:

CL.sub.int=Dose/AUC=(C0/CD)/(AUD+clast/k)×1000/60

C0: initial concentration in the incubation [μM], CD: cell density of vital cells [10e6 cells/ml], AUD: area under the data [μM×h], clast: concentration of last data point [μM], k: slope of the regression line for parent decline [h.sup.−1].

[0171] The calculated in vitro hepatic intrinsic clearance can be scaled up to the intrinsic in vivo hepatic clearance and used to predict hepatic in vivo blood clearance (CL) by the use of a liver model (well-stirred model).

Evaluation of Plasma Protein Binding

[0172] The equilibrium dialysis (ED) technique is used to determine the approximate in vitro fractional binding of test compounds to plasma proteins applying Dianorm Teflon dialysis cells (micro 0.2). Each dialysis cell consists of a donor and an acceptor chamber, separated by an ultrathin semipermeable membrane with a 5 kDa molecular weight cutoff. Stock solutions for each test compound are prepared in DMSO at 1 mM and serially diluted to obtain a final test concentration of 1 μM. The subsequent dialysis solutions are prepared in plasma (supplemented with NaEDTA as anticoagulant), and aliquots of 200 μl test compound dialysis solution in plasma are dispensed into the donor (plasma) chambers. Aliquots of 200 μl dialysis buffer (100 mM potassium phosphate, pH 7.4) are dispensed into the buffer (acceptor) chamber. Incubation is carried out for 2 hours under rotation at 37° C. for establishing equilibrium.

[0173] At the end of the dialysis period, aliquots obtained from donor and acceptor chambers, respectively, are transferred into reaction tubes, spiked with Internal Standard solution and processed for HPLC-MS/MS analysis. Analyte concentrations are quantified in aliquots of samples by HPLC-MS/MS against external calibration curves.

[0174] Percent bound is calculated using the formula:

% bound=(plasma concentration−buffer concentration/plasma concentration)×100

Evaluation of Solubility

[0175] The aqueous solubility of the test compound is determined by comparing the amount dissolved in buffer to the amount in an ACN/water (1/1) solution. Starting from a 10 mM DMSO stock solution aliquots are diluted with acetonitrile/water (1/1) or buffer resp. After 24 h of shaking, the solutions are filtrated and analyzed by LC-UV. The amount dissolved in buffer is compared to the amount in the ACN solution.

[0176] Solubility will usually be measured from 0.001 to 0.125 mg/mL at a DMSO concentration of 2.5%. If more than 90% of the compound is dissolved in buffer, the value is marked with “>”.

Evaluation of Pharmacokinetic Characteristics in Rodents

[0177] The test compound is administered either intravenously to fed rats or orally to fasted rats. Blood samples are taken at several time points post application of the test compound, anticoagulated and centrifuged.

[0178] The concentration of analytes—the administered compound and/or metabolites—are quantified in the plasma samples. PK parameters are calculated using non compartment methods. AUC and Cmax are normalized to a dose of 1 μmol/kg.

Evaluation of Inhibition of Cytochrome P450 Isoenzyme-Catalysed Reactions

[0179] The inhibition of cytochrome P450 isoenzyme-catalysed reactions by the test compound is assayed at 37° C. with human liver microsomes. All assays are carried out on a robotic system in 384-well plates. Test compounds are directly spotted into incubation plates from DMSO stocks by acoustic liquid dispensing (using the Labyte ECHO® system). The final incubation volume contains TRIS buffer (0.1 M), MgCl.sub.2 (5 mM), human liver microsomes, specific cytochrome P450 isoenzyme-substrate and the test compound at five different concentrations or no compound (high control) in duplicate (e.g. highest concentration 50 μM with subsequent serial 1:4 dilutions). Following a short preincubation period, reactions are started with the cofactor (NADPH, 1 mM) and stopped by cooling the incubation down to 8° C. and subsequently by addition of one volume of acetonitrile. An internal standard solution—usually the stable isotope of the formed metabolite—is added after quenching of incubations. Peak area analyte (=metabolite formed) and internal standard is determined by LC-MS/MS. The resulting peak area ratio analyte to internal standard in these incubations is compared to a control activity containing no test compound. Within each of the assay runs, the IC.sub.50 of a positive control inhibitor is determined. Experimental IC.sub.50 values are calculated by least square regression according to the following equation:

% control activity=(100% control activity/(1+(I/IC.sub.50)S)))−b

with
I=inhibitor concentration
S=slope factor
B=background activity

[0180] If the inhibition of the reaction is already >50% at the lowest concentration of the test compound, the IC.sub.50 is assigned “< lowest concentration tested” (usually <0.2 μM). If the inhibition of the reaction is still <50% at the highest concentration of the test compound, the IC50 is assigned “> highest concentration tested” (usually >50 μM).

Evaluation of Mechanism-Based Inhibition (MBI) of Cytochrome P450 3A4-Catalysed Midazolam Turnover

[0181] The mechanism-based inhibition towards CYP3A4 is assayed in human liver microsomes (0.02 mg/ml) with Midazolam (15 uM) as a substrate.

[0182] The test compounds are preincubated at 37° C. in presence of NADPH with human liver microsomes (0.2 mg/ml) at a concentration of 5 uM and 25 uM for 0 min, 10 min or 30 min. After preincubation, the incubate is diluted 1:10 and the substrate Midazolam is added for the main incubation (15 min). The main incubation is quenched with acetonitrile and the formation of Hydroxy-Midazolam is quantified via LC/MS-MS.

[0183] The turnover rates in pmol/min/mg protein are calculated and the activity after 10 and 30 min preincubation time is compared to that of the 0 min preincubation of the respective compound/concentration (% CTRL=% of the 0 min control of the respective compound/concentration). Additionally, the turnover rate is expressed relative to the turnover rate of the substrate reaction without compound added (% TR=% of the turnover rate without compound), in order to recognize competitive inhibition effects.

Methods of Treatment

[0184] In another aspect of the present invention, it is found that compounds of formula (I) or pharmaceutically acceptable salts thereof possess suitable properties for use in therapy and/or prevention, i.e. for use as medicaments. In particular, compounds of formula (I) or pharmaceutically acceptable salts thereof, as well as pharmaceutical compositions containing the same, may be useful for the treatment, i.e. therapy and/or prevention (prophylaxis), of diseases or conditions, which can be influenced by the inhibition of plasma kallikrein, e.g. which are mediated by unwanted PKK activity or in which inhibition of PKK is beneficial, in a patient.

[0185] Diseases and conditions which can be influenced by the inhibition of PKK, e.g. which are mediated by unwanted PKK activity or in which inhibition of PKK is beneficial, are, for instance, those mentioned in section Background of the Invention, in particular diabetic complications, diabetic retinopathy, proliferative and non-proliferative retinopathy, diabetic macular edema (DME), clinically significant macular edema (CSME), cystoid macular edema (CME), CME following cataract extraction, CME induced by cryotherapy, CME induced by uveitis, endophthalmitis, CME following vascular occlusion (e.g. central retinal vein occlusion, branch retinal vein occlusion, or hemiretinal vein occlusion), retinal edema, complications related to cataract surgery in diabetic retinopathy, hypertensive retinopathy, retinal trauma, dry and wet age-related macular degeneration (AMD), polypoidal choroidal vasculopathy (PCV), choroidal neovascularization (CNV; e.g. non-exudative choroidal neovascularization), hereditary angioedema (HAE), acute respiratory distress syndrome (ARDS), hemorrhage and edema after stroke, e.g. brain edema after stroke, vascular dementia, Alzheimer's disease, fibrotic disease, colitis, arthritis and renal injury.

[0186] Thus, the compounds and pharmaceutical compositions of the present invention are particularly suitable for treating ocular diseases including diabetic retinopathy, proliferative and non-proliferative retinopathy, diabetic macular edema (DME), retinal vein occlusion, age-related macular degeneration (AMD), polypoidal choroidal vasculopathy (PCV) and choroidal neovascularization (CNV; e.g. non-exudative choroidal neovascularization).

[0187] In addition, the compounds and pharmaceutical compositions according to the invention are particularly suitable for the treatment of edema, such as hereditary angioedema (HAE) and brain edema after stroke.

[0188] In particular, the compounds and pharmaceutical compositions according to the invention are suitable for the treatment of diabetic retinopathy, proliferative and non-proliferative retinopathy, diabetic macular edema (DME), age-related macular degeneration (AMD), polypoidal choroidal vasculopathy (PCV), choroidal neovascularization (CNV), hereditary angioedema (HAE), and brain edema after stroke.

[0189] The compounds and pharmaceutical compositions according to the invention are most particularly suitable for treating diabetic macular edema (DME), wet age-related macular degeneration (AMD), non-exudative choroidal neovascularization (CNV), hereditary angioedema (HAE), and brain edema after stroke.

[0190] For instance, they are particularly suitable for the prevention of diabetic macular edema (DME), wet age-related macular degeneration (AMD), hereditary angioedema (HAE), and brain edema after stroke as well as for the prevention of the conversion from non-exudative choroidal neovascularization (neCNV) to exudative choroidal neovascularization (eCNV).

[0191] The dose range of the compounds of formula (I) applicable per day is usually from 0.01 to 10 mg per kg body weight. The actual therapeutically effective amount or therapeutic dosage will of course depend on factors known by those skilled in the art such as age and weight of the patient, route of administration and severity of disease. In any case the compound or composition will be administered at dosages and in a manner which allows a therapeutically effective amount to be delivered based upon patient's unique condition.

[0192] The compounds and compositions, including any combinations with one or more additional therapeutic agents, according to the invention may be administered by oral, intravitreal, transdermal, inhalative, parenteral or sublingual route. Of the possible methods of administration, oral or intravitreal administration is preferred. In case of intravitreal injection the preferred dose should not exceed 5 mg per eye.

[0193] The patient to be treated is preferably a mammal, most preferably a human patient.

[0194] Thus, in another aspect, the present invention provides a compound of formula (I) and its tautomers, including pharmaceutically acceptable salts thereof, for use as a medicament.

[0195] In another aspect, the present invention provides a method for the treatment of a disease or condition, which is mediated by unwanted plasma kallikrein activity or in which inhibition of plasma kallikrein is beneficial, in a patient in need thereof.

[0196] Likewise, the present invention provides a compound of formula (I) and/or its tautomers or a pharmaceutically acceptable salt thereof for use in a method for the treatment of a disease or condition, which is mediated by unwanted plasma kallikrein activity or in which inhibition of plasma kallikrein is beneficial, in a patient in need thereof.

[0197] Likewise, the present invention provides the use of a compound of formula (I) and/or its tautomers or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for use in a method for the treatment of a disease or condition, which is mediated by unwanted plasma kallikrein activity or in which inhibition of plasma kallikrein is beneficial, in a patient in need thereof.

[0198] Likewise, the present invention provides the use of a compound of formula (I) and/or its tautomers or a pharmaceutically acceptable salt thereof, in a method for the treatment of a disease or condition, which is mediated by unwanted plasma kallikrein activity or in which inhibition of plasma kallikrein is beneficial, in a patient in need thereof.

[0199] According to one embodiment, the method for the treatment comprises administering to the patient one or more compounds of formula (I) and/or its tautomers or pharmaceutically acceptable salts thereof, preferably administering to the patient a therapeutically effective amount of one or more compounds of formula (I) and/or its tautomers or pharmaceutically acceptable salts thereof.

[0200] According to another embodiment, the method for the treatment comprises administering to the patient a pharmaceutical composition according to the present invention.

[0201] According to one embodiment, the disease or condition, which is mediated by unwanted plasma kallikrein activity or in which inhibition of plasma kallikrein is beneficial, is selected from ophthalmic indications such as diabetic retinopathy, proliferative and non-proliferative retinopathy, diabetic macular edema (DME), age-related macular degeneration (AMD), polypoidal choroidal vasculopathy (PCV) and choroidal neovascularization (CNV).

[0202] According to another embodiment, the disease or condition, which is mediated by unwanted plasma kallikrein activity or in which inhibition of plasma kallikrein is beneficial, is selected from edema-associated diseases such as hereditary angioedema (HAE) and brain edema after stroke.

[0203] According to another embodiment, the disease or condition, which is mediated by unwanted plasma kallikrein activity or in which inhibition of plasma kallikrein is beneficial, is selected from diabetic complications such as retinal vascular permeability associated with diabetic retinopathy and diabetic macular edema.

[0204] According to one embodiment, the patient is a human patient.

Pharmaceutical Compositions

[0205] In another aspect of the present invention, it is described that a compound of the invention or a pharmaceutically acceptable salt thereof may be used as active ingredients in pharmaceutical compositions.

[0206] Suitable preparations for administering the compounds of the invention, optionally in combination with one or more further therapeutic agents, will be apparent to those with ordinary skill in the art and include for example tablets, pills, capsules, suppositories, lozenges, troches, solutions, syrups, elixirs, sachets, injectables, inhalatives and powders etc. Oral formulations, particularly solid forms such as e.g. tablets or capsules are preferred. For intravitreal injection, solutions are preferred. The content of the pharmaceutically active compound(s) is advantageously in the range from 0.1 to 90 wt.-%, for example from 1 to 70 wt.-% of the composition as a whole.

[0207] Suitable tablets may be obtained, for example, by mixing one or more compounds according to formula (I) with known excipients, for example inert diluents, carriers, disintegrants, adjuvants, surfactants, binders and/or lubricants. The tablets may also consist of several layers. The particular excipients, carriers and/or diluents that are suitable for the desired preparations will be familiar to the skilled person on the basis of his specialist knowledge. The preferred ones are those that are suitable for the particular formulation and method of administration that are desired. The preparations or formulations according to the invention may be prepared using methods known per se that are familiar to the skilled person, such as for example by mixing or combining at least one compound of formula (I) according to the invention, or a pharmaceutically acceptable salt of such a compound, and one or more excipients, carriers and/or diluents.

[0208] Thus, according to another aspect of the present invention, pharmaceutical compositions comprising one or more compounds of formula (I) and/or their tautomers, or pharmaceutically acceptable salts thereof, optionally together with one or more inert carriers and/or diluents are provided.

[0209] Also, a pharmaceutical composition that comprises one or more of the above-mentioned compounds, or pharmaceutically acceptable salts thereof, optionally together with one or more inert carriers and/or diluents is provided for use in a method for the treatment of diseases or conditions which are mediated by unwanted plasma kallikrein activity or in which inhibition of plasma kallikrein is beneficial, in a patient in need thereof.

[0210] In particular, the invention provides a pharmaceutical composition according to this invention for use in a method for the treatment of ophthalmic indications such as diabetic retinopathy, proliferative and non-proliferative retinopathy, diabetic macular edema (DME), age-related macular degeneration (AMD), polypoidal choroidal vasculopathy (PCV) and choroidal neovascularization (CNV) and of edema-associated diseases such as hereditary angioedema (HAE) and brain edema after stroke.

[0211] Furthermore, the present invention relates to the use of a pharmaceutical composition according to this invention for the treatment of diseases or conditions which are mediated by unwanted plasma kallikrein activity in a patient, preferably in a human.

[0212] Also, the present invention relates to the use of a pharmaceutical composition according to this invention for the treatment of diseases or conditions in which inhibition of plasma kallikrein is beneficial in a patient, preferably in a human.

[0213] According to another embodiment, a pharmaceutical composition comprising one or more compounds of formula (I) and/or their tautomers, or pharmaceutically acceptable salts thereof, and one or more additional therapeutic agents, optionally together with one or more inert carriers and/or diluents is provided.

[0214] Preferably, this composition comprises one compound of formula (I) and/or its tautomers or a pharmaceutically acceptable salt thereof and one or more additional therapeutic agents.

Combination Therapy

[0215] The compounds of the invention may further be combined with one or more, preferably one additional therapeutic agent.

[0216] According to one embodiment the additional therapeutic agent is selected from the group of therapeutic agents useful in the treatment of diseases or conditions described hereinbefore, in particular associated with metabolic diseases or conditions such as for example diabetes mellitus, obesity, diabetic complications, hypertension, hyperlipidemia, or therapeutic agents useful for the treatment of ocular diseases.

[0217] Additional therapeutic agents which are suitable for such combinations include in particular those which for example potentiate the therapeutic effect of one or more active substances with respect to one of the indications mentioned and/or which allow the dosage of one or more active substances to be reduced.

[0218] Therefore a compound of the invention may be combined with one or more additional therapeutic agents selected from the group consisting of antidiabetic agents, agents for the treatment of overweight and/or obesity, agents for the treatment of high blood pressure, heart failure and/or atherosclerosis and agents for the treatment of ocular diseases. Antidiabetic agents are for example metformin, sulphonylureas, nateglinide, repaglinide, thiazolidinediones, PPAR-(alpha, gamma or alpha/gamma) agonists or modulators, alpha-glucosidase inhibitors, DPPIV inhibitors, SGLT2-inhibitors, insulin and insulin analogues, GLP-1 and GLP-1 analogues or amylin and amylin analogues, cycloset, 11β-HSD inhibitors. Other suitable combination partners are inhibitors of protein tyrosinephosphatase 1, substances that affect deregulated glucose production in the liver, such as e.g. inhibitors of glucose-6-phosphatase, or fructose-1,6-bisphosphatase, glycogen phosphorylase, glucagon receptor antagonists and inhibitors of phosphoenol pyruvate carboxykinase, glycogen synthase kinase or pyruvate dehydrokinase, alpha2-antagonists, CCR-2 antagonists or glucokinase activators. One or more lipid lowering agents are also suitable as combination partners, such as for example HMG-CoA-reductase inhibitors, fibrates, nicotinic acid and the derivatives thereof, PPAR-(alpha, gamma or alpha/gamma) agonists or modulators, PPAR-delta agonists, ACAT inhibitors or cholesterol absorption inhibitors such as, bile acid-binding substances such as, inhibitors of ileac bile acid transport, MTP inhibitors, or HDL-raising compounds such as CETP inhibitors or ABC1 regulators.

[0219] Therapeutic agents for the treatment of overweight and/or obesity are for example antagonists of the cannabinoid1 receptor, MCH-1 receptor antagonists, MC4 receptor agonists, NPY5 or NPY2 antagonists, β3-agonists, leptin or leptin mimetics, agonists of the 5HT2c receptor.

[0220] Therapeutic agents for the treatment of high blood pressure, chronic heart failure and/or atherosclerosis are for example A-II antagonists or ACE inhibitors, ECE inhibitors, diuretics, β-blockers, Ca-antagonists, centrally acting antihypertensives, antagonists of the alpha-2-adrenergic receptor, inhibitors of neutral endopeptidase, thrombocyte aggregation inhibitors and others or combinations thereof are suitable. Angiotensin II receptor antagonists are preferably used for the treatment or prevention of high blood pressure and complications of diabetes, often combined with a diuretic such as hydrochlorothiazide.

[0221] Therapeutic agents for the treatment of ocular diseases may include for example intravitreally administered corticosteroids, intravitreally administered anti-VEGF therapy, anti-Ang2 inhibitors, dual anti-VEGF/anti-Ang2 inhibitors, anti PDGF, dual anti-VEGF/anti-PDGF, VAP-1 (AOC3) inhibitors, Complement inhibitors (e.g. Complement factors 3, 5, B, and D inhibitors), Bradykinin receptor 1 antagonists, CCR-2 antagonists.

[0222] Additional treatments for ocular diseases may include laser coagulation therapy.

[0223] Preferably, compounds of the present invention and/or pharmaceutical compositions comprising a compound of the present invention optionally in combination with one or more additional therapeutic agents are administered in conjunction with exercise and/or a diet.

[0224] The dosage for the combination partners mentioned above is usually 1/5 of the lowest dose normally recommended up to 1/1 of the normally recommended dose.

[0225] The use of the compound according to the invention in combination with the additional therapeutic agent may take place simultaneously or at staggered times.

[0226] The compound according to the invention and the one or more additional therapeutic agents may both be present together in one formulation, for example a tablet or capsule, or separately in two identical or different formulations, for example as a so-called kit-of-parts.

[0227] Thus, according to another aspect, this invention relates to a pharmaceutical composition which comprises one or more compounds according to the invention and one or more additional therapeutic agents described hereinbefore and hereinafter, optionally together with one or more inert carriers and/or diluents.

[0228] According to another aspect, the present invention provides a method for the treatment of a disease or condition, which is mediated by unwanted plasma kallikrein activity or in which inhibition of plasma kallikrein is beneficial, in a patient in need thereof, the method comprising administering to the patient one or more compounds of formula (I) and/or its tautomers or pharmaceutically acceptable salts thereof, in combination with one or more additional therapeutic agents described in hereinbefore and hereinafter,

preferably administering to the patient a therapeutically effective amount of one or more compounds of formula (I) and/or its tautomers or pharmaceutically acceptable salts thereof, in combination with a therapeutically effective amount of one or more additional therapeutic agents described in hereinbefore and hereinafter.

[0229] Likewise, the present invention provides a compound of formula (I) and/or its tautomers or a pharmaceutically acceptable salt thereof in combination with one or more additional therapeutic agents described hereinbefore or hereinafter for use in a method for the treatment of a disease or condition, which is mediated by unwanted plasma kallikrein activity or in which inhibition of plasma kallikrein is beneficial, in a patient in need thereof.

[0230] Likewise, the present invention provides the use of a compound of formula (I) and/or its tautomers or a pharmaceutically acceptable salt thereof, in combination with one or more additional therapeutic agents described hereinbefore or hereinafter, in the manufacture of a medicament for use in a method for the treatment of a disease or condition, which is mediated by unwanted plasma kallikrein activity or in which inhibition of plasma kallikrein is beneficial, in a patient in need thereof.

[0231] Likewise, the present invention provides the use of a compound of formula (I) and/or its tautomers or a pharmaceutically acceptable salt thereof, in combination with one or more additional therapeutic agents described hereinbefore or hereinafter, in a method for the treatment of a disease or condition, which is mediated by unwanted plasma kallikrein activity or in which inhibition of plasma kallikrein is beneficial, in a patient in need thereof.

[0232] According to one embodiment, the method for the treatment comprises administering to the patient one or more compounds of formula (I) and/or its tautomers or pharmaceutically acceptable salts thereof, in combination with one or more additional therapeutic agents described in hereinbefore and hereinafter,

[0233] According to another embodiment, the method for the treatment comprises administering to the patient a pharmaceutical composition comprising one or more compounds according to the invention and one or more additional therapeutic agents described hereinbefore and hereinafter, optionally together with one or more inert carriers and/or diluents.

[0234] According to one embodiment, the one or more additional therapeutic agents are selected from antidiabetic agents, agents for the treatment of overweight and/or obesity, agents for the treatment of high blood pressure, heart failure and/or atherosclerosis and agents for the treatment of ocular diseases, in particular from those agents specifically mentioned above.

[0235] According to one embodiment, the disease or condition, which is mediated by unwanted plasma kallikrein activity or in which inhibition of plasma kallikrein is beneficial, is selected from ophthalmic indications such as diabetic retinopathy, proliferative and non-proliferative retinopathy, diabetic macular edema (DME), age-related macular degeneration (AMD), polypoidal choroidal vasculopathy (PCV) and choroidal neovascularization (CNV);

from edema-associated diseases such as hereditary angioedema (HAE) and brain edema after stroke; or from diabetic complications such as retinal vascular permeability associated with diabetic retinopathy and diabetic macular edema.

[0236] According to one embodiment, the patient is a human patient.

[0237] Other features and advantages of the present invention will become apparent from the following more detailed Examples which illustrate, by way of example, the principles of the invention.

Examples and Experimental Data

[0238] The following examples are for the purpose of illustration of the invention only and are not intended in any way to limit the scope of the present invention.

Abbreviations:

[0239] Ac acetyl [0240] ACN acetonitrile [0241] AMC 7-amino-4-methylcoumarin [0242] Boc tert-butyloxycarbonyl [0243] BSA bovine serum albumin [0244] Bzl benzyl [0245] d day(s) [0246] DAD diode array detector [0247] DBAD di-tert-butyl azodicarboxylate [0248] DBU 1,8-diazabicyclo[5.4.0]undec-7-ene [0249] DBN 1,5-diazabicyclo[4.3.0]non-5-ene [0250] DCM dichloromethane [0251] DEAD diethyl azodicarboxylate [0252] DIAD diisopropyl azodicarboxylate [0253] DIPEA N,N-diisopropylethylamine [0254] DMEM Dulbecco's modified eagle medium [0255] DMF N,N-dimethylformamide [0256] DMSO dimethyl sulfoxide [0257] EDTA ethylenediaminetetraacetate [0258] ESI electrospray ionization (in MS) [0259] EtOAc ethyl acetate [0260] EtOH ethanol [0261] h hour(s) [0262] HATU O-(7-azabenzotriazol-1-yl)-N,N,N′,N′-tetramethyluronium-hexafluorophosphate [0263] HPLC high performance liquid chromatography [0264] HPLC-MS coupled high performance liquid chromatography-mass spectrometry [0265] LC liquid chromatography [0266] LC-MS coupled liquid chromatography—mass spectrometry [0267] LG leaving group [0268] M molar (mol/L) [0269] MeOH methanol [0270] min minute(s) [0271] MS mass spectrometry [0272] NADPH nicotinamide adenine dinucleotide phosphate [0273] NMP N-methyl-2-pyrrolidone [0274] NMR nuclear magnetic resonance [0275] PET polyethylene terephthalate [0276] PyBop (benzotriazol-1-yloxy)tripyrrolidinophosphonium hexafluorophosphate [0277] R.sub.f retardation factor [0278] RFU relative fluorescence units [0279] RP reverse phase [0280] rt room temperature [0281] t.sub.R retention time (in HPLC/LC) [0282] SFC supercritical fluid chromatography [0283] TBTU O-(benzotriazol-1-yl)-N,N,N′,N′-tetramethyluronium tetrafluoroborate [0284] TFA trifluoroacetic acid [0285] THF tetrahydrofuran [0286] UV ultraviolet

[0287] The terms “ambient temperature” and “room temperature” are used interchangeably and designate a temperature of about 20° C., e.g. 15 to 25° C.

[0288] As a rule, .sup.1H-NMR and/or mass spectra have been obtained for the compounds prepared.

[0289] Unless otherwise specified, compounds containing chiral centers have the stereochemistry depicted. The assignment of stereochemistry has been made either by use of a chiral starting material of known stereochemistry, by stereoselective synthesis of known stereochemistry or by biological activity.

Analytical Methods

[0290]

TABLE-US-00006 Method: 1 Device: Agilent 1200 with DA- and MS-Detector Column: XBridge C18, 3 × 30 mm, 2.5 μm Column Supplier: Waters Gradient/Solvent % Solvent % Solvent Flow Temperature Time [min] [H.sub.2O, 0.1% NH3] [ACN] [mL/min] [° C.] 0.00 97 3 2.2 60 0.20 97 3 2.2 60 1.20 0 100 2.2 60 1.25 0 100 3 60 1.40 0 100 3 60

TABLE-US-00007 Method: 2 Device: Agilent 1200 with DA- and MS-Detector Column: Sunfire C18, 3 × 30 mm, 2.5 μm Column Supplier: Waters Gradient/Solvent % Solvent % Solvent Flow Temperature Time [min] [H.sub.2O, 0.1% TFA] [ACN] [mL/min] [° C.] 0.00 97 3 2.2 60 0.20 97 3 2.2 60 1.20 0 100 2.2 60 1.25 0 100 3 60 1.40 0 100 3 60

TABLE-US-00008 Method: 3 Device: Agilent 1260 SFC with DA- and MS-Detector Column: CHIRAL ART ® Cellulose SC, 4.6 × 250 mm, 5 μm Column Supplier: YMC % Solvent Gradient/Solvent % Solvent [MeOH, Flow Temperature Back pressure Time [min] [scCO.sub.2] 20 mM NH.sub.3] [mL/min] [° C.] [PSI] 0.00 65.0 35.0 4.0 40.0 2175.0 10.0 65.0 35.0 4.0 40.0 2175.0

TABLE-US-00009 Method: 4 Device: Waters Acquity, QDa Detector Column: XBridge C18, 3 × 30 mm, 2.5 μm Column Supplier: Waters Gradient/Solvent % Solvent % Solvent Flow Temperature Time [min] [H.sub.2O, 0.1% NH3] [ACN] [mL/min] [° C.] 0.00 95 5 1.5 60 1.30 0 100 1.5 60 1.50 0 100 1.5 60 1.60 95 5 1.5 60

TABLE-US-00010 Method: 5 Device: Waters Acquity, QDa Detector Column: XBridge C18, 3 × 30 mm, 2.5 μm Column Supplier: Waters Gradient/Solvent % Solvent % Solvent Flow Temperature Time [min] [H.sub.2O, 0.1% NH3] [ACN] [mL/min] [° C.] 0.00 95 5 1.5 60 1.30 0 100 1.5 60 1.50 0 100 1.5 60 1.60 95 5 1.5 60

Synthesis of Intermediates:

[0291] The starting materials and intermediates that are used in the processes leading to the compounds according to the invention are either commercially available or they may be prepared by methods (or by analogous or similar methods to those) described in the following or already known to those skilled in the art from the literature, e.g. from WO 2017/072020, WO 2017/072021 and WO 2018/192866 which are hereby incorporated by reference in their entirety.

Intermediate 1

(4R)-1-Methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-amine dihydrochloride

[0292] ##STR00045##

Step 1: Methyl 5-bromo-1-methyl-1H-imidazole-4-carboxylate

[0293] Under argon atmosphere methyl 1-methyl-1H-imidazole-4-carboxylate (19.1 g) is dissolved in DCM (230 mL). N-Bromosuccinimide (29.1 g), sodium persulfate (Na.sub.2S.sub.2O.sub.8, 64.9 g) and palladium-II-acetate (Pd(OAc).sub.2, 3.1 g) are added successively and the mixture is cooled to 0° C. Trifluoromethanesulfonic acid (CF.sub.3—SO.sub.3H, 42.2 mL) is added dropwise and thereafter the mixture is heated to 60° C. for 20 h. The mixture is diluted with DCM, cooled to 0° C. and treated carefully with saturated aqueous Na.sub.2CO.sub.3 until a pH-value of 8 is reached. The mixture is partitioned between water and DCM. The aqueous phase is extracted with DCM and the combined organic phases are dried (MgSO.sub.4) and concentrated in vacuo. The residue is chromatographed on silica gel (EtOAc/MeOH 85:15.fwdarw.70:30). The product thus obtained is triturated with tert.-butyl-methyl-ether (50 mL) to give the title compound.

[0294] LC (Method 1): t.sub.R=0.69 min; Mass spectrum (ESI.sup.+): m/z=219 [M+H].sup.+.

Step 2: Methyl 5-(3-methoxy-3-oxopropyl)-1-methyl-1H-imidazole-4-carboxylate

[0295] Methyl 5-bromo-1-methyl-1H-imidazole-4-carboxylate (10.6 g) is dissolved in dimethylacetamide (80 mL) and water (20 mL). 3,3-Dimethoxyprop-1-ene (8.6 mL) and N-methyldicyclohexylamin (15.3 mL) are added and the mixture is purged for 5 minutes with argon. Dichlorobis(tri-o-tolylphosphine)palladium(II) (PdCl.sub.2[P(o-Tol).sub.3].sub.2, 1.9 g) is added and the mixture is stirred for 3 h at 120° C. Then the mixture is partitioned between water and EtOAc and filtered over celite. The aqueous phase is mixed with saturated aqueous NaHCO.sub.3 and extracted for 4 times with EtOAc. The combined organic phases are dried (MgSO.sub.4) and concentrated in vacuo. The residue is chromatographed on silica gel (EtOAc/MeOH 90:10.fwdarw.70:30) to give the title compound.

[0296] LC (Method 2): t.sub.R=0.55 min; Mass spectrum (ESI.sup.+): m/z=227 [M+H].sup.+.

Step 3: Methyl 1-methyl-4-oxo-1H,4H,5H,6H-cyclopenta[d]imidazole-5-carboxylate

[0297] Under argon atmosphere methyl 5-(3-methoxy-3-oxopropyl)-1-methyl-1H-imidazole-4-carboxylate (4.5 g) is dissolved in THF (100 mL) and treated with potassium bis(trimethylsilyl)amide (40 mL of a 1 M solution in THF). The mixture is stirred for 30 minutes and then poured into a cooled mixture of EtOAc (800 mL) and acetic acid (7 mL). After stirring for 40 minutes the mixture is filtered. The solvents are evaporated in vacuo to give the title compound.

[0298] LC (Method 1): t.sub.R=0.25 min; Mass spectrum (ESI.sup.+): m/z=195 [M+H].sup.+.

Step 4: 1-Methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-one

[0299] A solution of methyl 1-methyl-4-oxo-1H,4H,5H,6H-cyclopenta[d]imidazole-5-carboxylate (4.0 g) in 1,4-dioxane (150 mL) and water (15 mL) is heated under reflux for 90 h. The solvents are evaporated in vacuo to give the title compound. LC (Method 1): t.sub.R=0.16 min; Mass spectrum (ESI.sup.+): m/z=137 [M+H].sup.+.

[0300] Alternatively, the reaction can be conducted by heating the starting material in a mixture of hydrogen chloride and acetic acid at 120° C. After completion of the reaction the solvents are evaporated in vacuo. The residue is dissolved in MeOH and treated with K.sub.2CO.sub.3 until a pH-value of 8 is reached. The mixture is filtered, concentrated and chromatographed on silica gel (DCM/MeOH 20:1.fwdarw.5:1) to give the title compound.

Step 5: (R)-2-Methyl-N-[(4E)-1-methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-ylidene]propane-2-sulfinamide

[0301] 1-Methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-one (2.94 g), toluene (120 mL) and titanium tetraethoxide (Ti(OEt).sub.4, 13.6 mL) are stirred for 15 minutes and then treated with (R)-2-methylpropane-2-sulfinamide (5.25 g). The mixture is heated for 4 h at reflux, cooled to rt and treated with saturated aqueous NaCl (30 mL). The mixture is stirred for 1 h and then filtered over celite. For two times the filter cake is stirred 10 minutes in MeOH (20 mL) and filtered over celite. The combined organic phases are concentrated and the residue is chromatographed on silica gel (DCM/MeOH 95:5). The product thus obtained is triturated from EtOAc to give the title compound.

[0302] LC (Method 3): t.sub.R=3.69 min; Mass spectrum (ESI.sup.+): m/z=240 [M+H].sup.+.

Step 6: (R)-2-Methyl-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]propane-2-sulfinamide

[0303] L-Selectride (1 M in THF, 45 mL) is dissolved in THF (75 mL) and treated portionwise with (R)-2-methyl-N-[(4E)-1-methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-ylidene]propane-2-sulfinamide (7.2 g). The mixture is stirred for 1 h and then treated dropwise with MeOH (5 mL). The solvents are evaporated in vacuo and the residue is chromatographed on silica gel (DCM/MeOH 95:5.fwdarw.80:20) to give the title compound.

[0304] LC (Method 2): t.sub.R=0.55 min; Mass spectrum (ESI.sup.+): m/z=242 [M+H].sup.+. LC (Method 3): t.sub.R=3.71 min.

Step 7: (4R)-1-Methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-amine dihydrochloride

[0305] A mixture of (R)-2-methyl-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]propane-2-sulfinamide (7.25 g) in isopropanol (50 mL) is treated with a 1.25 M solution of HCl in isopropanol (50 mL) and stirred for 2 h. The precipitate is collected by filtration, washed successively with isopropanol and diethylether and dried in vacuo to give the title compound. Mass spectrum (ESI.sup.+): m/z=138 [M+H].sup.+.

Intermediate 2

5-{3-Azabicyclo[3.1.0]hexan-3-yl}pyridine-2-carbaldehyde

[0306] ##STR00046##

[0307] 5-Fluoropyridine-2-carbaldehyde (1.0 g), 3-azabicyclo[3.1.0]hexane hydrochloride (1.1 g) and K.sub.2CO.sub.3 (2.8 g) are suspended in NMP (10 mL) and heated to 120° C. for 2 h. The mixture is cooled to rt, partitioned between water and EtOAc and the phases are separated. The aqueous phase is extracted twice with EtOAc, the combined organic phase are dried (MgSO.sub.4) and concentrated. The residue is chromatographed on silica gel (cyclohexane/EtOAc 100:0.fwdarw.60:40) to give the title compound. LC (Method 2): t.sub.R=0.68 min; Mass spectrum (ESI+): m/z=189 [M+H].sup.+.

[0308] Intermediates 2-1 to 2-4 are prepared in analogy to Intermediate 2:

TABLE-US-00011 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M + H].sup.+ LC Method 2-1 [00047] embedded image 0.75 189 Method 2 2-2 [00048] 0.73 225 Method 2 2-3 [00049] 0.82 189 Method 2 2-4 [00050] 1.22 301 Method 1

TABLE-US-00012 Intermediate Reaction comment 2-2 The reaction is conducted for 12 h at 115° C. 2-3 The reaction is conducted in DMF for 18 h at rt. 2-4 The reaction is conducted in DMF for 26 h at 80° C.

TABLE-US-00013 Name of Name of Intermediate Name Starting Material 1 Starting Material 2 2-1 5-{5-Azaspiro[2.3]hexan-5- 5-Fluoropyridine-2- 5-Azaspiro[2.3]hexane yl}pyridine-2-carbaldehyde carbaldehyde hemioxalate 2-2 5-{6,6-Difluoro-3-azabicyclo- 5-Fluoropyridine-2- 6,6-Difluoro-3-azabicyclo- [3.1,0]hexan-3-yl}pyridine-2- carbaldehyde [3.1.0]hexane hydrochloride carbaldehyde 2-3 6-{3-Azabicyclo[3.1.0]hexan- 6-Fluoropyridine-3- 3-Azabicyclo[3.1.0]hexane 3-yl}pyridine-3-carbaldehyde carbaldehyde hydrochloride 2-4 3-(5-Iodo-6-methylpyridin-2- 6-Fluoro-3-iodo-2- 3-Azabicyclo[3.1.0]hexane yl)-3-azabicyclo[3.1.0]hexane methylpyridine hydrochloride

Intermediate 3

(5-{3-Azabicyclo[3.1.0]hexan-3-yl}pyridin-2-yl)methanol

[0309] ##STR00051##

[0310] NaBH.sub.4 (217 mg) is added portionwise to a ice-cooled mixture of 5-{3-azabicyclo[3.1.0]hexan-3-yl}pyridine-2-carbaldehyde (920 mg) in THF (10 mL) and MeOH (5 mL). The mixture is stirred for 1 h at 0° C., treated with 1 M aqueous HCl (10 mL) and stirred for 30 minutes at rt. Then the mixture is partitioned between saturated aqueous NaHCO.sub.3 and EtOAc. The phases are separated. The organic phase is dried (MgSO.sub.4) and concentrated to give the title compound. LC (Method 2): t.sub.R=0.59 min; Mass spectrum (ESI+): m/z=191 [M+H].sup.+.

[0311] Intermediates 3-1 to 3-23 are prepared in analogy to Intermediate 3:

TABLE-US-00014 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M + H].sup.+ LC Method 3-1 [00052] embedded image 0.94 305 Method 2 3-2 [00053] 0.90 261 Method 2 3-3 [00054] 0.87 225 Method 2 3-4 [00055] 0.90 269 Method 2 3-5 [00056] 1.00 259 Method 2 3-6 [00057] 0.99 223 Method 1 3-7 [00058] 1.03 223 Method 2 3-8 [00059] 0.60 191 Method 2 3-9 [00060] 0.89 225 Method 1 3-10 [00061] 0.94 269 Method 2 3-11 [00062] embedded image 0.52 227 Method 2 3-12 [00063] 0.59 241 Method 2 3-13 [00064] 0.58 227 Method 2 3-14 [00065] 0.82 205 Method 1 3-15 [00066] 0.85 205 Method 1 3-16 [00067] 0.87 225 Method 1 3-17 [00068] 0.83 266 Method 2 3-18 [00069] 0.82 205 Method 1 3-19 [00070] 0.62 255 Method 2 3-20 [00071] 0.71 230 Method 2 3-21 [00072] embedded image 0.96 239 Method 2 3-22 [00073] 0.60 219 Method 2 3-23 [00074] 0.79 227 Method 2

TABLE-US-00015 Intermediate Reaction comment 3-1 The reaction is conducted in EtOH. 3-5 Methylether is formed during stirring in presence of 1N aqueous HCl. 3-6 Methylether is formed during stirring in presence of 1N aqueous HCl. 3-7 Methylether is formed during stirring in presence of 1N aqueous HCl. 3-10 The reaction is conducted in EtOH. 3-12 The reaction is conducted in EtOH. 3-14 The reaction is conducted in THF/EtOH 1:2. 3-15 The reaction is conducted in EtOH for 2 h at rt. 3-16 The reaction is conducted for 2 h at 0° C. 3-17 The reaction is conducted for 30 minutes at rt. 3-18 The reaction is conducted in EtOH for 1 h at rt. 3-19 The reaction is conducted for 45 minutes at rt. 3-20 The reaction is conducted for 45 minutes at 0° C. 3-21 The reaction is conducted for 1 h at 0° C. 3-22 The reaction is conducted in EtOH for 1.5 h at rt. 3-23 The reaction is conducted in EtOH for 1 h at rt. Intermediate Name Name of Starting Material 3-1 (2-Bromo-6-{6,6-difluoro-3-azabicyclo- 2-Bromo-6-{6,6-difluoro-3-azabicyclo- [3.1.0]hexan-3-yl}pyridin-3-yl)methanol [3.1.0]hexan-3-yl}pyridine-3-carbaldehyde 3-2 (2-Chloro-6-{6,6-difluoro-3-azabicyclo- 2-Chloro-6-{6,6-difluoro-3-azabicyclo- [3.1.0]hexan-3-yl}pyridin-3-yl)methanol [3.1.0]hexan-3-yl}pyridine-3-carbaldehyde 3-3 (6-{5-Azaspiro[2.3]hexan-5-yl}-2-chloropyridin- 6-{5-Azaspiro[2.3]hexan-5-yl}-2- 3-yl)methanol chloropyridine-3-carbaldehyde 3-4 (6-{5-Azaspiro[2.3]hexan-5-yl}-2- 6-{5-Azaspiro[2.3]hexan-5-yl}-2- bromopyridin-3-yl)methanol bromopyridine-3-carbaldehyde 3-5 6,6-Difluoro-3-[6-fluoro-5-(methoxy- 6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}- methyl)pyridin-2-yl]-3-azabicyclo[3.1.0]hexane 2-fluoropyridine-3-carbaldehyde 3-6 5-[6-Fluoro-5-(methoxymethyl)pyridin-2-yl]-5- 6-{5-Azaspiro[2.3]hexan-5-yl}-2-fluoropyridine- azaspiro[2.3]hexane 3-carbaldehyde 3-7 3-[6-Fluoro-5-(methoxymethyl)pyridin-2-yl]-3- 6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- azabicyclo[3.1.0]hexane fluoropyridine-3-carbaldehyde 3-8 (5-{5-Azaspiro[2.3]hexan-5-yl}pyridin-2- 5-{5-Azaspiro[2.3]hexan-5-yl}pyridine-2- yl)methanol carbaldehyde 3-9 (6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- 6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- chloropyridin-3-yl)methanol chloropyridine-3-carbaldehyde 3-10 (6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- 6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- bromopyridin-3-yl)methanol bromopyridine-3-carbaldehyde 3-11 (6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- 6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- yl}pyridin-3-yl)methanol yl}pyridine-3-carbaldehyde 3-12 (6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- 6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}- yl}-2-methylpyridin-3-yl)methanol 2-methylpyridine-3-carbaldehyde 3-13 (5-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- 5-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- yl}pyridin-2-yl)methanol yl}pyridine-2-carbaldehyde 3-14 (6-{5-Azaspiro[2.3]hexan-5-yl}-2- 6-{5-Azaspiro[2.3]hexan-5-yl}-2- methylpyridin-3-yl)methanol methylpyridine-3-carbaldehyde 3-15 (6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- 6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- methylpyridin-3-yl)methanol methylpyridine-3-carbaldehyde 3-16 (6-{3-Azabicyclo[3.1.0]hexan-3-yl}-4- 6-{3-Azabicyclo[3.1.0]hexan-3-yl}-4- chloropyridin-3-yl)methanol chloropyridine-3-carbaldehyde 3-17 2-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}- 2-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}- 5-(hydroxymethyl)-4-methylpyridine-3- 5-formyl-4-methylpyridine-3-carbonitrile carbonitrile 3-18 (6-{3-Azabicyclo[3.1.0]hexan-3-yl}-4- 6-{3-Azabicyclo[3.1.0]hexan-3-yl}-4- methylpyridin-3-yl)methanol methylpyridine-3-carbaldehyde 3-19 (6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- 6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}- yl}-2,4-dimethylpyridin-3-yl)methanol 2,4-dimethylpyridine-3-carbaldehyde 3-20 2-{3-Azabicyclo[3.1.0]hexan-3-yl}-5-(hydroxy- 2-{3-Azabicyclo[3.1.0]hexan-3-yl}-5-formyl-4- methyl)-4-methylpyridine-3-carbonitrile methylpyridine-3-carbonitrile 3-21 {2-Chloro-6-[(1R,5S,6R)-6-methyl-3-aza- 2-Chloro-6-[(1R,5S,6R)-6-methyl-3- bicyclo[3.1.0]hexan-3-yl]pyridin-3-yl}methanol azabicyclo[3.1.0]hexan-3-yl]pyridine-3-carbaldehyde 3-22 (6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- 6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- ethylpyridin-3-yl)methanol ethylpyridine-3-carbaldehyde 3-23 (6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3- 6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}- yl}pyridin-3-yl)methanol pyridine-3-carbaldehyde

Intermediate 4

Ethyl 1-[(5-β-azabicyclo[3.1.0]hexan-3-yl)pyridin-2-yl)methyl]-1H-imidazole-4-carboxylate

[0312] ##STR00075##

[0313] In a microwave vial a mixture of (5-{3-azabicyclo[3.1.0]hexan-3-yl}pyridin-2-yl)methanol (100 mg), ethyl 1H-imidazole-4-carboxylate (77 mg) and p-toluenesulfonic add (54 mg) in ACN (15 mL) is heated for 5 h to 120° C. After cooling to rt the mixture is partitioned between saturated aqueous NaHCO.sub.3 and EtOAc. The aqueous phase is extracted with EtOAc and the combined organic phases are dried (MgSO.sub.4) and concentrated. The residue is purified by HPLC on reversed phase (ACN, water) to give the title compound.

[0314] LC (Method 2): t.sub.R=0.71 min; Mass spectrum (ESI+): m/z=313 [M+H].sup.+.

[0315] Intermediates 4-1 to 4-61 are prepared in analogy to Intermediate 4:

TABLE-US-00016 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M + H].sup.+ LC Method 4-1 [00076] embedded image 1.02 384 Method 2 4-2 [00077] 1.03 348 Method 2 4-3 [00078] 1.05 392 Method 2 4-4 [00079] 0.85 367 Method 2 4-5 [00080] 0.94 314 Method 1 4-6 [00081] 0.76 313 Method 2 4-7 [00082] 1.00 332 Method 2 4-8 [00083] 0.95 331 Method 1 4-9 [00084] 1.06 331 Method 2 4-10 [00085] 1.11 391 Method 2 4-11 [00086] embedded image 1.04 428 Method 2 4-12 [00087] 1.06 427 Method 2 4-13 [00088] 0.99 391 Method 1 4-14 [00089] 0.78 388 Method 1 4-15 [00090] 1.01 332 Method 2 4-16 [00091] 1.04 367 Method 2 4-17 [00092] 0.97 347 Method 1 4-18 [00093] 1.08 347 Method 2 4-19 [00094] 0.99 368 Method 2 4-20 [00095] 0.98 427 Method 1 4-21 [00096] embedded image 0.93 416 Method 2 4-22 [00097] 1.08 392 Method 2 4-23 [00098] 0.83 356 Method 2 4-24 [00099] 1.07 348 Method 2 4-25 [00100] 0.68 350 Method 2 4-26 [00101] 0.98 327 Method 1 4-27 [00102] 0.95 363 Method 1 4-28 [00103] 1.01 397 Method 2 4-29 [00104] 0.92 391 Method 2 4-30 [00105] 1.14 391 Method 2 4-31 [00106] embedded image 0.78 349 Method 2 4-32 [00107] 1.10 383 Method 2 4-33 [00108] 1.05 347 Method 2 4-34 [00109] 0.72 349 Method 2 4-35 [00110] 0.97 383 Method 1 4-36 [00111] 1.21 397 Method 2 4-37 [00112] 0.90 Method 1 4-38 [00113] 0.91 347 Method 2 4-39 [00114] 0.92 313 Method 1 4-40 [00115] 1.07 338 Method 1 4-41 [00116] embedded image 1.05 347 Method 1 4-42 [00117] 1.02 388 Method 1 4-43 [00118] 0.92 388 Method 1 4-44 [00119] 1.09 331 Method 2 4-45 [00120] 0.93 327 Method 1 4-46 [00121] 0.98 377 Method 1 4-47 [00122] 0.79 377 Method 2 4-48 [00123] 1.05 361 Method 1 4-49 [00124] 1.03 338 Method 2 4-50 [00125] 0.97 383 Method 1 4-51 [00126] embedded image 1.04 352 Method 2 4-52 [00127] 0.94 352 Method 1 4-53 [00128] 0.96 347 Method 1 4-54 [00129] 1.00 381 Method 1 4-55 [00130] 1.11 Method 1 4-56 [00131] 1.17 405 Method 2 4-57 [00132] 0.89 364 Method 1 4-58 [00133] 0.90 350 Method 1 4-59 [00134] 0.85 328 Method 2 4-60 [00135] 0.93 327 Method 2 4-61 [00136] embedded image 0.89 328 Method 2

TABLE-US-00017 Intermediate Reaction comment 4-1 The reaction is conducted for 10 minutes at 70° C. 4-2 The reaction is conducted for 15 minutes at 70° C. 4-3 The reaction is conducted for 15 minutes at 70° C. 4-4 The reaction is conducted for 48 h at 90° C. 4-5 The reaction is conducted for 10 h at 130° C. 4-6 The reaction is conducted for 4 h at 120° C. 4-7 The reaction is conducted for 10 minutes at 70° C. 4-8 The reaction is conducted for 5 h at 70° C. 4-9 The reaction is conducted for 1 h at 70° C. 4-10 The reaction is conducted for 30 minutes at 80° C. 4-11 The reaction is conducted for 10 minutes at 70° C. 4-12 The reaction is conducted for 2 h at 80° C. 4-13 The reaction is conducted for 12 h at 80° C. 4-14 The reaction is conducted for 4 h at 80° C. 4-15 The reaction is conducted for 10 minutes at 70° C. 4-16 The reaction is conducted for 1 h at 70° C. 4-17 The reaction is conducted for 15 h at 80° C. 4-18 The reaction is conducted for 1 h at 70° C. 4-19 The reaction is conducted for 10 minutes at 70° C. 4-20 The reaction is conducted for 7 h at 80° C. and for 2 h at 90° C. 4-21 Camphersulfonic acid is used instead of p-toluenesulfonic acid. The reaction is conducted for 4 h at 80° C. 4-22 The reaction is conducted for 15 minutes at 70° C. 4-23 The reaction is conducted for 2 h at 90° C. 4-24 The reaction is conducted for 15 minutes at 70° C. 4-25 The reaction is conducted for 12 h at 70° C. 4-26 The reaction is conducted for 5 h at 90° C. 4-27 The reaction is conducted for 15 h at 90° C. 4-28 The reaction is conducted for 12 h at 90° C. 4-29 The reaction is conducted for 5 h at 80° C. 4-30 The reaction is conducted for 30 minutes at 80° C. 4-31 The reaction is conducted for 6 h at 120° C. 4-32 The reaction is conducted for 2 h at 80° C. 4-33 The reaction is conducted for 2 h at 60° C. 4-34 The reaction is conducted for 4 h at 120° C. and for 1 h at 130° C. 4-35 The reaction is conducted for 15 h at 75° C. and for 12 h at 80° C. 4-36 The reaction is conducted for 30 minutes at 70° C. 4-37 The reaction is conducted for 12 h at 70° C. and for 6 h at 80° C. 4-38 The reaction is conducted for 12 h at 80° C. 4-39 The reaction is conducted for 12 h at 90° C. 4-40 The reaction is conducted for 12 h at 80° C. 4-41 The reaction is conducted for 22 h at 70° C. 4-42 The reaction is conducted for 12 h at 90° C. 4-43 The reaction is conducted for 48 h at 80° C. 4-44 The reaction is conducted for 3 h at 70° C. 4-45 The reaction is conducted for 20 h at 80° C. 4-46 The reaction is conducted for 4 h at 80° C. 4-47 The reaction is conducted for 2 h at 80° C. 4-48 The reaction is conducted for 20 h at 80° C. 4-49 The reaction is conducted for 5 h at 70° C. 4-50 The reaction is conducted for 5 h at 90° C. 4-51 The reaction is conducted for 16 h at 60° C. 4-52 The reaction is conducted for 12 h at 90° C. 4-53 The reaction is conducted for 22 h at 70° C. 4-54 The reaction is conducted for 22 h at 70° C. 4-55 The reaction is conducted for 20 h at 70° C. 4-56 The reaction is conducted for 12 h at 70° C. 4-57 The reaction is conducted for 4 h at 90° C. 4-58 The reaction is conducted for 13 h at 130° C. 4-59 The reaction is conducted for 5 h at 90° C. 4-60 The reaction is conducted for 5 h at 90° C. 4-61 The reaction is conducted for 5 h at 90° C.

TABLE-US-00018 Name of Name of Intermediate Name Starting Material 1 Starting Material 2 4-1 Ethyl 1-[(2-chloro-6-{6,6-difluoro-3- 2-Chloro-6-{6,6-difluoro-3- Ethyl 1H-1,2,3-triazole- azabicyclo[3.1.0]-hexan-3- azabicyclo[3.1.0]hexan-3- 4-carboxylate yl}pyridin-3-yl)methyl]-1H-1,2,3- yl}pyridin-3-yl)methanol triazole-4-carboxylate 4-2 Ethyl 1-[(6-{5-azaspiro[2.3]hexan- (6-{5-Azaspiro[2.3]hexan-5-yl}- Ethyl 1H-1,2,3-triazole- 5-yl}-2-chloropyridin-3-yl)methyl]- 2-chloropyridin-3-yl)methanol 4-carboxylate 1H-1,2,3-triazole-4-carboxylate 4-3 Ethyl 1-[(6-{5-azaspiro[2.3]hexan- (6-{5-Azaspiro[2.3]hexan-5-yl}- Ethyl 1H-1,2,3-triazole- 5-yl}-2-bromopyridin-3-yl)methyl]- 2-bromopyridin-3-yl)methanol 4-carboxylate 1H-1,2,3-triazole-4-carboxylate 4-4 Ethyl 1-[(6-{6,6-difluoro-3- 6,6-Difluoro-3-[6-fluoro-5- Ethyl 1H-imidazole-4- azabicyclo[3.1.0]hexan-3-yl}-2- (methoxymethyl)pyridin-2-yl]-3- carboxylate fluoropyridin-3-yl)methyl]-1H- azabicyclo[3.1.0]hexane imidazole-4-carboxylate 4-5 Ethyl 1-[(5-{3-azabicyclo[3.1.0]- (5-{3-Azabicyclo[3.1.0]hexan- Ethyl 1H-1,2,3-triazole- hexan-3-yl}pyridin-2-yl)methyl]-1H- 3-yl}pyridin-2-yl)methanol 4-carboxylate 1,2,3-triazole-4-carboxylate 4-6 Ethyl 1-[(5-{3-azabicyclo[3.1.0]- (5-{3-Azabicyclo[3.1.0]hexan- Ethyl 1H-pyrazole-4- hexan-3-yl}pyridin-2-yl)methyl]-1H- 3-yl}pyridin-2-yl)methanol carboxylate pyrazole-4-carboxylate 4-7 Ethyl 1-[(6-{5-azaspiro[2.3]hexan- 5-[6-Fluoro-5- Ethyl 1H-1,2,3-triazole- 5-yl}-2-fluoropyridin-3-yl)methyl]- (methoxymethyl)pyridin-2-yl]-5- 4-carboxylate 1H-1,2,3-triazole-4-carboxylate azaspiro[2.3]hexane 4-8 Ethyl 1-[(6-{5-azaspiro[2.3]hexan- 5-[6-Fluoro-5- Ethyl 1H-imidazole-4- 5-yl}-2-fluoropyridin-3-yl)methyl]- (methoxymethyl)pyridin-2-yl]-5- carboxylate 1H-imidazole-4-carboxylate azaspiro[2.3]hexane 4-9 Ethyl 1-[(6-{5-azaspiro[2.3]hexan- 5-[6-Fluoro-5- Ethyl 1H-pyrazole-4- 5-yl}-2-fluoropyridin-3-yl)methyl]- (methoxymethyl)pyridin-2-yl]-5- carboxylate 1H-pyrazole-4-carboxylate azaspiro[2.3]hexane 4-10 Ethyl 1-[(6-{5-azaspiro[2.3]hexan- (6-{5-Azaspiro[2.3]hexan-5-yl}- Ethyl 1H-pyrazole-4- 5-yl}-2-bromopyridin-3-yl)methyl]- 2-bromopyridin-3-yl)methanol carboxylate 1H-pyrazole-4-carboxylate 4-11 Ethyl 1-[(2-bromo-6-{6,6-difluoro-3- (2-Bromo-6-{6,6-difluoro-3- Ethyl 1H-1,2,3-triazole- azabicyclo[3.1.0]hexan-3-yl}pyridin- azabicyclo[3.1.0]hexan-3- 4-carboxylate 3-yl)methyl]-1H-1,2,3-triazole-4- yl}pyridin-3-yl)methanol carboxylate 4-12 Ethyl 1-[(2-bromo-6-{6,6-difluoro-3- (2-Bromo-6-{6,6-difluoro-3- Ethyl 1H-pyrazole-4- azabicyclo[3.1.0]hexan-3-yl}pyridin- azabicyclo[3.1.0]hexan-3- carboxylate 3-yl)methyl]-1H-pyrazole-4- yl}pyridin-3-yl)methanol carboxylate 4-13 Ethyl 1-[(6-{5-azaspiro[2.3]hexan- (6-{5-Azaspiro[2.3]hexan-5-yl}- Ethyl 1H-imidazole-4- 5-yl}-2-bromopyridin-3-yl)methyl]- 2-bromopyridin-3-yl)methanol carboxylate 1H-imidazole-4-carboxylate 4-14 1-[(2-{3-Azabicyclo[3.1.0]-hexan-3- (2-{3-Azabicyclo[3.1.0]hexan- 1H-imidazole-4- yl}-4-[(1E)-2-phenyl- 3-yl}-4-[(1E)-2-phenylethenyl]- carboxylic acid ethenyl]pyrimidin-5-yl)methyl]-1H- pyrimidin-5-yl)methanol imidazole-4-carboxylic acid 4-15 Ethyl 1-[(6-{3-azabicyclo[3.1.0]- 3-[6-Fluoro-5- Ethyl 1H-1,2,3-triazole- hexan-3-yl}-2-fluoropyridin-3-yl)- (methoxymethyl)pyridin-2-yl]-3- 4-carboxylate methyl]-1H-1,2,3-triazole-4- azabicyclo[3.1.0]hexane carboxylate 4-16 Ethyl 1-[(6-{6,6-difluoro-3- 6,6-Difluoro-3-[6-fluoro-5- Ethyl 1H-pyrazole-4- azabicyclo[3.1.0]hexan-3-yl}-2- (methoxymethyl)pyridin-2-yl]-3- carboxylate fluoropyridin-3-yl)methyl]-1H- azabicyclo[3.1.0]hexane pyrazole-4-carboxylate 4-17 Ethyl 1-[(6-{5-azaspiro[2.3]hexan- (6-{5-Azaspiro[2.3]hexan-5-yl}- Ethyl 1H-imidazole-4- 5-yl}-2-chloropyridin-3-yl)methyl]- 2-chloropyridin-3-yl)methanol carboxylate 1H-imidazole-4-carboxylate 4-18 Ethyl 1-[(6-{3-azabicyclo[3.1.0]- (6-{3-Azabicyclo[3.1.0]hexan- Ethyl 1H-pyrazole-4- hexan-3-yl}-2-chloropyridin-3-yl)- 3-yl}-2-chloropyridin-3-yl)- carboxylate methyl]-1H-pyrazole-4-carboxylate methanol 4-19 Ethyl 1-[(6-{6,6-difluoro-3- 6,6-Difluoro-3-[6-fluoro-5- Ethyl 1H-1,2,3-triazole- azabicyclo[3.1.0]hexan-3-yl}-2- (methoxymethyl)pyridin-2-yl]-3- 4-carboxylate fluoropyridin-3-yl)methyl]-1H-1,2,3- azabicyclo[3.1.0]hexane triazole-4-carboxylate 4-20 Ethyl 1-[(2-bromo-6-{6,6-difluoro-3- (2-Bromo-6-{6,6-difluoro-3- Ethyl 1H-imidazole-4- azabicyclo[3.1.0]-hexan-3-yl}- azabicyclo[3.1.0]hexan-3-yl}- carboxylate pyridin-3-yl)methyl]-1H-imidazole- pyridin-3-yl)methanol 4-carboxylate 4-21 Ethyl 1-[(2-{3-azabicyclo[3.1.0]- (2-{3-Azabicyclo[3.1.0]hexan- Ethyl 1H-imidazole-4- hexan-3-yl}-4-[(1E)-2-phenyl- 3-yl}-4-[(1E)-2-phenylethenyl]- carboxylate ethenyl]pyrimidin-5-yl)methyl]-1H- pyrimidin-5-yl)methanol imidazole-4-carboxylate 4-22 Ethyl 1-[(6-{3-azabicyclo[3.1.0]- (6-{3-Azabicyclo[3.1.0]hexan- Ethyl 1H-1,2,3-triazole- hexan-3-yl}-2-bromopyridin-3-yl)- 3-yl}-2-bromopyridin-3-yl)- 4-carboxylate methyl]-1H-1,2,3-triazole-4- methanol carboxylate 4-23 Ethyl 1-[(6-{3-azabicyclo[3.1.0]- (6-{3-Azabicyclo[3.1.0]hexan- Ethyl 1H-imidazole-4- hexan-3-yl}-2-propylpyridin-3-yl)- 3-yl}-2-propylpyridin-3-yl)- carboxylate methyl]-1H-imidazole-4-carboxylate methanol 4-24 Ethyl 1-[(6-{3-azabicyclo[3.1.0]- (6-{3-Azabicyclo[3.1.0]hexan- Ethyl 1H-1,2,3-triazole- hexan-3-yl}-2-chloropyridin-3-yl)- 3-yl}-2-chloropyridin-3-yl)- 4-carboxylate methyl]-1H-1,2,3-triazole-4- methanol carboxylate 4-25 Ethyl 1-[(6-{6,6-difluoro-3- (6-{6,6-Difluoro-3- Ethyl 1H-1,2,3-triazole- azabicyclo[3.1.0]hexan-3-yl}pyridin- azabicyclo[3.1.0]hexan-3-yl}- 4-carboxylate 3-yl)methyl]-1H-1,2,3-triazole-4- pyridin-3-yl)methanol carboxylate 4-26 Ethyl 1-[(6-{3-azabicyclo[3.1.0]- (6-{3-Azabicyclo[3.1.0]hexan- Ethyl 1H-imidazole-4- hexan-3-yl}-2-methylpyridin-3-yl)- 3-yl}-2-methylpyridin-3-yl)- carboxylate methyl]-1H-imidazole-4-carboxylate methanol 4-27 Ethyl 1-[(6-{6,6-difluoro-3- (6-{6,6-Difluoro-3- Ethyl 1H-imidazole-4- azabicyclo[3.1.0]hexan-3-yl}-2- azabicyclo[3.1.0]hexan-3-yl}-2- carboxylate methylpyridin-3-yl)methyl]-1H- methylpyridin-3-yl)methanol imidazole-4-carboxylate 4-28 Ethyl 1-[(6-{3-azabicyclo[3.1.0]- 3-[5-(Methoxymethyl)-6- Ethyl 1H-imidazole-4- hexan-3-yl}-2-(trifluoromethoxy)- (trifluoromethoxy)pyridin-2-yl]- carboxylate pyridin-3-yl)methyl]-1H-imidazole- 3-azabicyclo[3.1.0]hexane 4-carboxylate 4-29 Ethyl 1-[(6-{3-azabicyclo[3.1.0]- (6-{3-Azabicyclo[3.1.0]hexan- Ethyl 1H-imidazole-4- hexan-3-yl}-2-bromopyridin-3-yl)- 3-yl}-2-bromopyridin-3-yl)- carboxylate methyl]-1H-imidazole-4-carboxylate methanol 4-30 Ethyl 1-[(6-{3-azabicyclo[3.1.0]- (6-{3-Azabicyclo[3.1.0]hexan- Ethyl 1H-pyrazole-4- hexan-3-yl}-2-bromopyridin-3-yl)- 3-yl}-2-bromopyridin-3-yl)- carboxylate methyl]-1H-pyrazole-4-carboxylate methanol 4-31 Ethyl 1-[(5-{6,6-difluoro-3- (5-{6,6-Difluoro-3- Ethyl 1H-pyrazole-4- azabicyclo[3.1.0]hexan-3-yl}pyridin- azabicyclo[3.1.0]hexan-3-yl}- carboxylate 2-yl)methyl]-1H-pyrazole-4- pyridin-2-yl)methanol carboxylate 4-32 Ethyl 1-[(2-chloro-6-{6,6-difluoro-3- (2-Chloro-6-{6,6-difluoro-3- Ethyl 1H-pyrazole-4- azabicyclo[3.1.0]-hexan-3-yl}- azabicyclo[3.1.0]hexan-3-yl}- carboxylate pyridin-3-yl)methyl]-1H-pyrazole-4- pyridin-3-yl)methanol carboxylate 4-33 Ethyl 1-[(6-{5-azaspiro[2.3]hexan- (6-{5-Azaspiro[2.3]hexan-5-yl}- Ethyl 1H-pyrazole-4- 5-yl}-2-chloropyridin-3-yl)methyl]- 2-chloropyridin-3-yl)methanol carboxylate 1H-pyrazole-4-carboxylate 4-34 Ethyl 1-[(5-{6,6-difluoro-3- (5-{6,6-Difluoro-3- Ethyl 1H-imidazole-4- azabicyclo[3.1.0]hexan-3-yl}pyridin- azabicyclo[3.1.0]hexan-3-yl}- carboxylate 2-yl)methyl]-1H-imidazole-4- pyridin-2-yl)methanol carboxylate 4-35 Ethyl 1-[(2-chloro-6-{6,6-difluoro-3- (2-Chloro-6-{6,6-difluoro-3- Ethyl 1H-imidazole-4- azabicyclo[3.1.0]-hexan-3-yl}- azabicyclo[3.1.0]hexan-3-yl}- carboxylate pyridin-3-yl)methyl]-1H-imidazole- pyridin-3-yl)methanol 4-carboxylate 4-36 Ethyl 1-[(6-{3-azabicyclo[3.1.0]- 3-[5-(Methoxymethyl)-6- Ethyl 1H-pyrazole-4- hexan-3-yl}-2-(trifluoro- (trifluoromethoxy)pyridin-2-yl]- carboxylate methoxy)pyridin-3-yl)methyl]-1H- 3-azabicyclo[3.1.0]hexane pyrazole-4-carboxylate 4-37 Ethyl 1-[(6-{6,6-difluoro-3- (6-{6,6-Difluoro-3- Ethyl 1H-imidazole-4- azabicyclo[3.1.0]hexan-3-yl}pyridin- azabicyclo[3.1.0]hexan-3- carboxylate 3-yl)methyl]-1H-imidazole-4- yl}pyridin-3-yl)methanol carboxylate 4-38 Ethyl 1-[(6-{3- (6-{3-Azabicyclo[3.1.0]hexan- Ethyl 1H-imidazole-4- azabicyclo[3.1.0]hexan-3-yl}-2- 3-yl}-2-chloropyridin-3- carboxylate chloropyridin-3-yl)methyl]-1H- yl)methanol imidazole-4-carboxylate 4-39 Ethyl 1-[(6-{3-azabicyclo[3.1.0]- (6-{3-Azabicyclo[3.1.0]hexan- Ethyl 1H-imidazole-4- hexan-3-yl}pyridin-3-yl)methyl]-1H- 3-yl}pyridin-3-yl)methanol carboxylate imidazole-4-carboxylate 4-40 Methyl 1-[(6-{3-azabicyclo- (6-{3-Azabicyclo[3.1.0]hexan- Methyl 3-cyano-1H- [3.1.0]hexan-3-yl}-2-methyl-pyridin- 3-yl}-2-methylpyridin-3- pyrazole-4-carboxylate 3-yl)methyl]-3-cyano-1H-pyrazole- yl)methanol 4-carboxylate 4-41 Ethyl 1-[(6-{3- (6-{3-Azabicyclo[3.1.0]hexan- Ethyl 1H-pyrazole-4- azabicyclo[3.1.0]hexan-3-yl}-4- 3-yl}-4-chloropyridin-3- carboxylate chloropyridin-3-yl)methyl]-1H- yl)methanol pyrazole-4-carboxylate 4-42 Ethyl 1-[(5-cyano-6-{6,6-difluoro-3- 2-{6,6-Difluoro-3- Ethyl 1H-pyrazole-4- azabicyclo[3.1.0]hexan-3-yl}-4- azabicyclo[3.1.0]hexan-3-yl}-5- carboxylate methylpyridin-3-yl)methyl]-1H- (hydroxymethyl)-4- pyrazole-4-carboxylate methylpyridine-3-carbonitrile 4-43 Ethyl 1-[(5-cyano-6-{6,6-difluoro-3- 2-{6,6-Difluoro-3- Ethyl 1H-imidazole-4- azabicyclo[3.1.0]hexan-3-yl}-4- azabicyclo[3.1.0]hexan-3-yl}-5- carboxylate methylpyridin-3-yl)methyl]-1H- (hydroxymethyl)-4- imidazole-4-carboxylate methylpyridine-3-carbonitrile 4-44 Ethyl 1-[(6-{3-azabicyclo[3.1.0]- 3-[6-Fluoro-5- Ethyl 1H-pyrazole-4- hexan-3-yl}-2-fluoropyridin-3-yl)- (methoxymethyl)pyridin-2-yl]-3- carboxylate methyl]-1H-pyrazole-4-carboxylate azabicyclo[3.1.0]hexane 4-45 Ethyl 1-[(6-{3-azabicyclo[3.1.0]- (6-{3-Azabicyclo[3.1.0]hexan- Ethyl 1H-imidazole-4- hexan-3-yl}-4-methylpyridin-3-yl)- 3-yl}-4-methylpyridin-3-yl)- carboxylate methyl]-1H-imidazole-4-carboxylate methanol 4-46 Ethyl 1-[(6-{6,6-difluoro-3- (6-{6,6-Difluoro-3- Ethyl 1H-imidazole-4- azabicyclo[3.1.0]hexan-3-yl}-2,4- azabicyclo[3.1.0]hexan-3-yl}- carboxylate dimethylpyridin-3-yl)methyl]-1H- 2,4-dimethylpyridin-3-yl)- imidazole-4-carboxylate methanol 4-47 Ethyl 1-[(6-{6,6-difluoro-3- (6-{6,6-Difluoro-3- Ethyl 1H-pyrazole-4- azabicyclo[3.1.0]hexan-3-yl}-2,4- azabicyclo[3.1.0]hexan-3-yl}- carboxylate dimethylpyridin-3-yl)methyl]-1H- 2,4-dimethylpyridin-3-yl)- pyrazole-4-carboxylate methanol 4-48 Ethyl 1-({2-chloro-6-[(1R,5S,6R)-6- {2-Chloro-6-[(1R,5S,6R)-6- Ethyl 1H-imidazole-4- methyl-3-azabicyclo[3.1.0]hexan-3- methyl-3-azabicyclo[3.1.0]- carboxylat yl]pyridin-3-yl}methyl)-1H- hexan-3-yl]pyridin-3-yl}- imidazole-4-carboxylate methanol 4-49 Ethyl 1-[(6-{3-azabicyclo[3.1.0]- 2-{3-Azabicyclo[3.1.0]hexan-3- Ethyl 1H-pyrazole-4- hexan-3-yl}-5-cyanopyridin-3-yl)- yl}-5-(hydroxymethyl)pyridine- carboxylate methyl]-1H-pyrazole-4-carboxylate 3-carbonitrile 4-50 Ethyl 2-chloro-1-[(6-{6,6-difluoro-3- (6-{6,6-difluoro-3- Ethyl 2-chloro-1H- azabicyclo[3.1.0]hexan-3-yl}pyridin- azabicyclo[3.1.0]hexan-3- imidazole-4-carboxylate 3-yl)methyl]-1H-imidazole-4- yl}pyridin-3-yl)methanol carboxylate 4-51 Ethyl 1-[(6-{3-azabicyclo[3.1.0]- 2-{3-Azabicyclo[3.1.0]hexan-3- Ethyl 1H-pyrazole-4- hexan-3-yl}-5-cyano-4- yl}-5-(hydroxymethyl)-4- carboxylate methylpyridin-3-yl)methyl]-1H- methylpyridine-3-carbonitrile pyrazole-4-carboxylate 4-52 Ethyl 1-[(6-{3-azabicyclo[3.1.0]- 2-{3-Azabicyclo[3.1.0]hexan-3- Ethyl 1H-imidazole-4- hexan-3-yl}-5-cyano-4- yl}-5-(hydroxymethyl)-4- carboxylate methylpyridin-3-yl)methyl]-1H- methylpyridine-3-carbonitrile imidazole-4-carboxylate 4-53 Ethyl 1-[(6-{3-azabicyclo[3.1.0]- (6-{3-Azabicyclo[3.1.0]hexan- Ethyl 1H-imidazole-4- hexan-3-yl}-4-chloropyridin-3-yl)- 3-yl}-4-chloropyridin-3- carboxylate methyl]-1H-imidazole-4-carboxylate yl)methanol 4-54 Ethyl 1-[(6-{3-azabicyclo[3.1.0]- (6-{3-Azabicyclo[3.1.0]hexan- Ethyl 1H-imidazole-4- hexan-3-yl}-4-(trifluoromethyl)- 3-yl}-4-(trifluoromethyl)pyridin- carboxylate pyridin-3-yl)methyl]-1H-imidazole- 3-yl)methanol 4-carboxylate 4-55 Ethyl 1-[(6-{3-azabicyclo[3.1.0]- (6-{3-Azabicyclo[3.1.0]hexan- Ethyl 1H-imidazole-4- hexan-3-yl}-5-bromo-2- 3-yl}-5-bromo-2-methylpyridin- carboxylate methylpyridin-3-yl)methyl]-1H- 3-yl)methanol imidazole-4-carboxylate 4-56 Ethyl 1-[(6-{3-azabicyclo[3.1.0]- (6-{3-Azabicyclo[3.1.0]hexan- Ethyl 1H-pyrazole-4- hexan-3-yl}-5-bromo-2- 3-yl}-5-bromo-2-methylpyridin- carboxylate methylpyridin-3-yl)methyl]-1H- 3-yl)methanol pyrazole-4-carboxylate 4-57 Ethyl 1-[(2-{6,6-difluoro-3- (2-{6,6-Difluoro-3- Ethyl 1H-imidazole-4- azabicyclo[3.1.0]hexan-3-yl}-4- azabicyclo[3.1.0]hexan-3-yl}-4- carboxylat methylpyrimidin-5-yl)methyl]-1H- methylpyrimidin-5-yl)methanol imidazole-4-carboxylate 4-58 Ethyl 1-[(5-{6,6-difluoro-3- (5-{6,6-Difluoro-3- Ethyl 1H-1,2,3-triazole- azabicyclo[3.1.0]hexan-3-yl}pyridin- azabicyclo[3.1.0]hexan-3-yl}- 4-carboxylate 2-yl)methyl]-1H-1,2,3-triazole-4- pyridin-2-yl)methanol carboxylate 4-59 Ethyl 1-[(6-{5-azaspiro[2.3]hexan- (2-{5-Azaspiro[2.3]hexan-5-yl}- Ethyl 1H-imidazole-4- 5-yl}-2-methylpyrimidin-3-yl)- 4-methylpyrimidin-5-yl)- carboxylat methyl]-1H-imidazole-4-carboxylate methanol 4-60 Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5- (2-{5-Azaspiro[2.3]hexan-5-yl}- Ethyl 1H-imidazole-4- yl}-2-methylpyridin-3-yl)methyl]-1H- 4-methylpyridin-5-yl)methanol carboxylate imidazole-4-carboxylate 4-61 Ethyl 1-[(6-{3-azabicyclo[3.1.0]- (6-{3-Azabicyclo[3.1.0]-hexan- Ethyl 1H-imidazole-4- hexan-3-yl}-2-methylpyrimidin-3- 3-yl}-2-methyl-pyrimidin-3-yl)- carboxylate yl)methyl]-1H-imidazole-4- methanol carboxylate

Intermediate 5

1-[(5-{3-Azabicyclo[3.1.0]hexan-3-yl}pyridin-2-yl)methyl]-1H-imidazole-4-carboxylic acid

[0316] ##STR00137##

[0317] A mixture of ethyl 1-[(5-{3-azabicyclo[3.1.0]hexan-3-yl}pyridin-2-yl)methyl]-1H-imidazole-4-carboxylate (162 mg), MeOH (1 mL), THF (1 mL) and KOH (4 M aqueous solution, 648 μL) is stirred for 3 h at 50° C. After cooling to rt aqueous HCl (4 M, 648 μL) is added and the solvents are evaporated to give the crude product, which is directly used in the next step. LC (Method 2): t.sub.R=0.58 min; Mass spectrum (ESI+): m/z=285 [M+H].sup.+.

[0318] Intermediates 5-1 to 5-170 are prepared in analogy to Intermediate 5:

TABLE-US-00019 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M + H].sup.+ LC Method 5-1 [00138] embedded image 0.67 365 Method 2 5-2 [00139] 0.90 356 Method 2 5-3 [00140] 0.91 336 Method 2 5-4 [00141] 0.89 320 Method 2 5-5 [00142] 0.93 364 Method 2 5-6 [00143] 0.73 Method 2 5-7 [00144] 0.62 286 Method 2 5-8 [00145] 0.64 285 Method 2 5-9 [00146] 0.86 304 Method 2 5-10 [00147] 0.73 Method 2 5-11 [00148] embedded image 0.90 303 Method 2 5-12 [00149] 0.94 363 Method 2 5-13 [00150] 0.91 400 Method 2 5-14 [00151] 0.94 399 Method 1 5-15 [00152] 0.77 Method 2 5-16 [00153] 0.88 304 Method 2 5-17 [00154] 0.89 339 Method 2 5-18 [00155] 0.66 285 Method 2 5-19 [00156] 0.62 314 Method 2 5-20 [00157] 0.62 Method 1 5.21 [00158] embedded image 0.63 313 Method 2 5-22 [00159] 0.86 362 [M +Na].sup.+ Method 2 5-23 [00160] 0.57 286 Method 2 5-24 [00161] 0.64 Method 1 5-25 [00162] 0.67 336 Method 2 5-26 [00163] 0.95 336 Method 2 5-27 [00164] 0.71 328 Method 1 5-28 [00165] 0.92 320 Method 2 5-29 [00166] 0.54 322 Method 2 5-30 [00167] embedded image 0.62 299 Method 1 5-31 [00168] 0.62 335 Method 1 5-32 [00169] 0.84 364 Method 2 5-33 [00170] 0.88 310 Method 2 5-34 [00171] 349 5-35 [00172] Crude product is directly used in the next step 5-36 [00173] 0.88 Method 2 5-37 [00174] 0.79 Method 2 5-38 [00175] 0.97 363 Method 2 5-39 [00176] embedded image 0.66 321 Method 2 5-40 [00177] 0.87 350 Method 2 5-41 [00178] 0.66 335 Method 1 5-42 [00179] 0.96 355 Method 2 5-43 [00180] 0.65 371 Method 1 5-44 [00181] 0.65 371 Method 1 5-45 [00182] 0.64 Method 1 5-46 [00183] 0.62 336 Method 1 5-47 [00184] 0.67 313 Method 1 5-48 [00185] 0.64 335 Method 1 5-49 [00186] embedded image 0.58 321 Method 2 5-50 [00187] 0.62 343 Method 2 5-51 [00188] 0.62 319 Method 1 5-52 [00189] 0.63 377 [M + Na].sup.+ Method 1 5-53 [00190] 1.06 369 Method 2 5-54 [00191] 0.65 343 Method 2 5-55 [00192] 0.51 286 Method 2 5-56 [00193] 0.56 316 Method 2 5-57 [00194] 0.66 316 Method 2 5-58 [00195] embedded image 0.55 321 Method 1 5-59 [00196] 0.66 335 Method 1 5-60 [00197] 1.04 352 Method 2 5-61 [00198] 0.60 363 Method 2 5-62 [00199] 0.73 375 Method 1 5-63 [00200] 0.59 300 Method 2 5-64 [00201] 0.70 363 Method 1 5-65 [00202] 0.41 286 Method 1 5-66 [00203] 0.28 285 Method 2 5-67 [00204] 0.40 313 Method 2 5-68 [00205] embedded image 0.73 349 Method 1 5-69 [00206] 0.60 315 Method 1 5-70 [00207] 0.76 324 Method 2 5-71 [00208] 0.61 329 Method 2 5-72 [00209] 0.60 329 Method 2 5-73 [00210] 0.60 329 Method 2 5-74 [00211] 0.63 343 Method 2 5-75 [00212] 0.58 329 Method 2 5-76 [00213] 0.71 343 Method 2 5-77 [00214] 0.59 315 Method 1 5-78 [00215] embedded image 0.77 316 Method 2 5-79 [00216] 0.62 335 Method 2 5-80 [00217] 0.33 316 Method 2 5-81 [00218] Crude product is directly used in the next step 5-82 [00219] 0.83 337 Method 2 5-83 [00220] 0.59 365 Method 2 5-84 [00221] 0.24 351 Method 2 5-85 [00222] 0.44 352 Method 2 5-86 [00223] 0.69 324 Method 1 5-87 [00224] embedded image 0.66 319 Method 2 5-88 [00225] 0.62 341 Method 2 5-89 [00226] 0.60 314 Method 2 5-90 [00227] 0.90 360 Method 2 5-91 [00228] 0.75 382 [M + Na].sup.+ Method 2 5-92 [00229] 0.92 303 Method 2 5-93 [00230] 0.65 299 Method 1 5-94 [00231] 0.59 365 Method 2 5-95 [00232] 0.64 338 Method 2 5-96 [00233] embedded image 0.75 Method 2 5-97 [00234] 0.63 350 Method 2 5-98 [00235] 0.57 327 Method 2 5-99 [00236] 0.64 349 Method 1 5-100 [00237] 0.67 349 Method 2 5-101 [00238] 0.72 351 Method 2 5-102 [00239] 0.86 310 Method 2 5-103 [00240] 0.64 352 Method 2 5-104 [00241] 0.87 324 Method 2 5-105 [00242] 0.70 325 Method 2 5-106 [00243] embedded image 0.73 322 [M − H].sup.− Method 2 5-107 [00244] 0.56 319 Method 2 5-108 [00245] 0.71 353 Method 2 5-109 [00246] 0.78 319 Method 2 5-110 [00247] 0.63 343 Method 2 5-111 [00248] 0.78 324 Method 2 5-112 [00249] 0.64 379 Method 2 5-113 [00250] 0.97 319 Method 2 5-114 [00251] 0.67 378 Method 2 5-115 [00252] embedded image 0.97 377 Method 2 5-116 [00253] 0.95 324 Method 2 5-117 [00254] 0.74 377 Method 1 5-118 [00255] 0.68 313 Method 2 5-119 [00256] 0.65 313 Method 2 5-120 [00257] 0,65 310 Method 1 5-121 [00258] 0.68 253 Method 2 5-122 [00259] 0.60 299 Method 2 5-123 [00260] 0.67 336 Method 2 5-124 [00261] embedded image 0.74 337 Method 2 5-125 [00262] 0.65 301 Method 2 5-126 [00263] 0.65 322 Method 2 5-127 [00264] 0.62 320 Method 2 5-128 [00265] 0.62 320 Method 2 5-129 [00266] 0.65 334 Method 2 5-130 [00267] 0.65 334 Method 2 5-131 [00268] 0.73 327 Method 2 5-132 [00269] 0.70 336 Method 1 5-133 [00270] embedded image 0.72 372 Method 1 5-134 [00271] 0.65 314 Method 2 5-135 [00272] 0.64 352 Method 1 5-136 [00273] 0.65 320 Method 1 5-137 [00274] 0.65 316 Method 1 5-138 [00275] 0.99 356 Method 2 5-139 [00276] 0.61 286 Method 1 5-140 [00277] 0.64 336 Method 2 5-141 [00278] 0.61 300 Method 2 5-142 [00279] embedded image 0.68 350 Method 2 5-143 [00280] 0.62 336 Method 2 5-144 [00281] 0.98 320 Method 2 5-145 [00282] 0.67 314 Method 2 5-146 [00283] 0.64 314 Method 2 5-147 [00284] 0.94 354 Method 2 5-148 [00285] 0.78 391 Method 2 5-149 [00286] 0.74 377 Method 2 5-150 [00287] 0.82 and 0.84 445 Method 2 [00288] embedded image 5-151 [00289] 0.73 341 Method 2 5-152 [00290] 0.71 363 Method 2 5-153 [00291] 0.70 327 Method 2 5-154 [00292] 0.65 313 Method 2 5-155 [00293] 0.71 327 Method 2 5-156 [00294] 0.67 313 Method 2 5-157 [00295] 0.58 300 Method 2 5-158 [00296] 0.63 299 Method 2 5-159 [00297] 0.60 300 Method 2 5-160 [00298] embedded image 0.65 355 Method 1 5-161 [00299] 0.63 350 Method 1 5-162 [00300] 0.77 387 Method 1 5-163 [00301] 0.68 351 Method 1 5-164 [00302] 0.67 299 Method 2 5-165 [00303] 0.68 299 Method 2 5-166 [00304] 0.69 335 Method 2 5-167 [00305] 0.72 349 Method 2 5-168 [00306] 0.72 329 Method 2 5-169 [00307] embedded image 0.75 365 Method 2 5-170 [00308] 0.69 367 Method 2

TABLE-US-00020 Intermediate Reaction comment 5-1 The reaction is conducted in EtOH for 3 h at rt. 5-2 NaOH is used instead of KOH. The reaction is conducted for 1.5 h at 70° C. 5-3 NaOH is used instead of KOH. The reaction is conducted in EtOH for 48 h at rt. The product is purified by HPLC on reversed phase (ACN, water). 5-4 NaOH is used instead of KOH. The reaction is conducted at 70° C. for 2 h. 5-5 NaOH is used instead of KOH. The reaction is conducted at 70° C. for 1 h. 5-6 NaOH is used instead of KOH. The reaction is conducted at 70° C. for 1.5 h. 5-7 NaOH is used instead of KOH. The reaction is conducted at 70° C. for 1.5 h. 5-9 NaOH is used instead of KOH. The reaction is conducted at 70° C. for 1.5 h. 5-10 NaOH is used instead of KOH. The reaction is conducted at 70° C. for 1.5 h. 5-11 NaOH is used instead of KOH. The reaction is conducted at 70° C. for 1.5 h. 5-12 NaOH is used instead of KOH. The reaction is conducted at 70° C. for 2 h. 5-13 NaOH is used instead of KOH. The reaction is conducted at 70° C. for 1 h. 5-14 NaOH is used instead of KOH. The reaction is conducted in THF/EtOH for 45 minutes at 70° C. 5-15 NaOH is used instead of KOH. The reaction is conducted at 70° C. for 1.5 h. 5-16 NaOH is used instead of KOH. The reaction is conducted at 70° C. for 1 h. 5-17 NaOH is used instead of KOH. The reaction is conducted at 70° C. for 1.5 h. 5-19 NaOH is used instead of KOH. The reaction is conducted in THF/EtOH for 2 h at 50° C. 5-20 NaOH is used instead of KOH. The reaction is conducted in THF/EtOH for 20 minutes at 70° C. 5-21 NaOH is used instead of KOH. The reaction is conducted in MeOH for 12 h at rt. 5-22 NaOH is used instead of KOH. The reaction is conducted at 70° C. for 1 h. 5-23 NaOH is used instead of KOH. The reaction is conducted at 70° C. for 0.1 h. 5-24 NaOH is used instead of KOH. The reaction is conducted in THF/EtOH for 45 minutes at 70° C. 5-25 NaOH is used instead of KOH. The reaction is conducted in THF/EtOH for 4 h at 50° C. 5-26 NaOH is used instead of KOH. The reaction is conducted at 70° C. for 1 h. 5-27 NaOH is used instead of KOH. The reaction is conducted in THF/EtOH for 2 h at 70° C. 5-28 NaOH is used instead of KOH. The reaction is conducted at 70° C. for 1 h. 5-29 The reaction is conducted at 70° C. for 1 h. 5-30 NaOH is used instead of KOH. The reaction is conducted in EtOH for 3 h at rt. 5-31 NaOH is used instead of KOH. The reaction is conducted for 1.5 h at 50° C. 5-32 NaOH is used instead of KOH. The reaction is conducted at 70° C. for 1 h. 5-33 NaOH is used instead of KOH. The reaction is conducted at 50° C. for 1 h. 5-34 NaOH is used instead of KOH. The reaction is conducted at 50° C. for 1.5 h. 5-35 NaOH is used instead of KOH. The reaction is conducted in THF/EtOH for 5 h at 50° C. 5-36 NaOH is used instead of KOH. The reaction is conducted at 70° C. for 1 h. 5-37 NaOH is used instead of KOH. The reaction is conducted at 70° C. for 1 h. 5-38 NaOH is used instead of KOH. The reaction is conducted at 70° C. for 3 h. 5-40 NaOH is used instead of KOH. The reaction is conducted at 50° C. for 1 h. 5-41 NaOH is used instead of KOH. The reaction is conducted at 70° C. for 8 h. 5-42 NaOH is used instead of KOH. The reaction is conducted at 40° C. for 4 h. 5-43 NaOH is used instead of KOH. The reaction is conducted in THF/EtOH for 45 minutes at 70° C. 5-44 NaOH is used instead of KOH. The reaction is conducted in THF/EtOH for 2 h at 70° C. 5-45 NaOH is used instead of KOH. The reaction is conducted in THF/EtOH for 45 minutes at 70° C. 5-46 The reaction is conducted in EtOH for 48 h at 50° C. 5-47 The reaction is conducted in EtOH for 4 h at 50° C. 5-48 NaOH is used instead of KOH. The reaction is conducted in THF/EtOH for 2 h at 70° C. 5-49 The reaction is conducted for 2 h at 50° C. 5-50 NaOH is used instead of KOH. The reaction is conducted in EtOH for 12 h at 40° C. 5-51 NaOH is used instead of KOH. The reaction is conducted in THF/EtOH for 1 h at 70° C. 5-52 NaOH is used instead of KOH. The reaction is conducted in THF/EtOH for 40 minutes at 70° C. 5-53 NaOH is used instead of KOH. The reaction is conducted at 70° C. for 2 h. 5-54 NaOH is used instead of KOH. The reaction is conducted in EtOH for 12 h at rt. 5-55 NaOH is used instead of KOH. The reaction is conducted at 70° C. for 5 h. 5-56 NaOH is used instead of KOH. The reaction is conducted at 50° C. for 2 h. 5-57 NaOH is used instead of KOH. The reaction is conducted in THF for 4 h at 80° C. 5-58 NaOH is used instead of KOH. The reaction is conducted in 1,4-dioxane for 3 h at 50° C. 5-59 NaOH is used instead of KOH. The reaction is conducted in THF/EtOH for 40 minutes at 70° C. 5-60 NaOH is used instead of KOH. The reaction is conducted in EtOH for 48 h at rt. 5-61 NaOH is used instead of KOH. The reaction is conducted at 70° C. for 1 h. 5-62 NaOH is used instead of KOH. The reaction is conducted in THF/EtOH for 2 h at 70° C. 5-63 NaOH is used instead of KOH. The reaction is conducted in THF/EtOH for 2 h at 50° C. 5-64 NaOH is used instead of KOH. The reaction is conducted in THF/EtOH for 2 h at 70° C. 5-65 NaOH is used instead of KOH. The reaction is conducted in THF/EtOH for 1.5 h at70° C. 5-66 NaOH is used instead of KOH. The reaction is conducted in MeOH for 3 h at rt. 5-67 NaOH is used instead of KOH. The reaction is conducted for 2 h at 60° C. 5-68 NaOH is used instead of KOH. The reaction is conducted for 12 h at 40° C. 5-69 NaOH is used instead of KOH. The reaction is conducted in MeOH for 4 h at rt. 5-70 NaOH is used instead of KOH. The reaction is conducted in MeOH for 15 h at rt. 5-71 NaOH is used instead of KOH. The reaction is conducted for 6 h at 50° C. 5-72 NaOH is used instead of KOH. The reaction is conducted in THF/EtOH for 2.5 h at 70° C. 5-73 NaOH is used instead of KOH. The reaction is conducted in THF/EtOH for 2.5 h at 70° C. 5-74 NaOH is used instead of KOH. The reaction is conducted in THF/EtOH for 2.5 h at 70° C. 5-75 NaOH is used instead of KOH. The reaction is conducted in MeOH for 12 h at 50° C. 5-76 NaOH is used instead of KOH. The reaction is conducted in THF/EtOH for 50 minutes at rt. 5-77 NaOH is used instead of KOH. The reaction is conducted in MeOH for 4 h at rt. 5-78 NaOH is used instead of KOH. The reaction is conducted for 4 h at 50° C. 5-79 The reaction is conducted in EtOH for 12 h at 50° C. 5-80 NaOH is used instead of KOH. The reaction is conducted in EtOH for 12 h at rt. 5-81 NaOH is used instead of KOH. The reaction is conducted in THF for 6 h at 80° C. and for 12 h at rt. 5-82 LiOH is used instead of KOH. The reaction is conducted in THF/MeOH for 12 h at 50° C. 5-83 NaOH is used instead of KOH. The reaction is conducted for 2 h at 40° C. 5-84 The reaction is conducted for 4 h at 40° C. 5-85 NaOH is used instead of KOH. The reaction is conducted in EtOH for 12 h at rt. 5-86 NaOH is used instead of KOH. The reaction is conducted in MeOH for 6 h at rt. 5-87 NaOH is used instead of KOH. The reaction is conducted for 3 h at 70° C. 5-88 NaOH is used instead of KOH. The reaction is conducted for 2 h at 70° C. 5-89 The reaction is conducted in EtOH for 12 h at 50° C. 5-90 NaOH is used instead of KOH. The reaction is conducted for 4.5 h at 50° C. 5-91 NaOH is used instead of KOH. The reaction is conducted for 2 h at 50° C. 5-92 The reaction is conducted for 12 h at 50° C. 5-93 NaOH is used instead of KOH. The reaction is conducted in EtOH for 2.5 h at rt. 5-94 NaOH is used instead of KOH. The reaction is conducted in 1,4-dioxane for 1 h at 90° C. 5-95 NaOH is used instead of KOH. The reaction is conducted in MeOH for 12 h at rt. 5-96 NaOH is used instead of KOH. The reaction is conducted for 1 h at 70° C. 5-97 The reaction is conducted in EtOH for 12 h at 50° C. 5-98 NaOH is used instead of KOH. The reaction is conducted for 2 h at 70° C. 5-99 NaOH is used instead of KOH. The reaction is conducted for 1 h at 70° C. 5-100 NaOH is used instead of KOH. The reaction is conducted for 1 h at 70° C. 5-101 NaOH is used instead of KOH. The reaction is conducted at 70° C. for 1 h. 5-102 NaOH is used instead of KOH. The reaction is conducted for 1 h at 70° C. 5-103 NaOH is used instead of KOH. The reaction is conducted at 70° C. for 1 h. 5-104 NaOH is used instead of KOH. The reaction is conducted for 1 h at 60° C. 5-105 NaOH is used instead of KOH. The reaction is conducted in EtOH for 12 h at rt. 5-106 NaOH is used instead of KOH. The reaction is conducted for 1 h at 60° C. 5-107 NaOH is used instead of KOH. The reaction is conducted for 2.5 h at 70° C. 5-108 NaOH is used instead of KOH. The reaction is conducted for 2.5 h at 70° C. 5-109 NaOH is used instead of KOH. The reaction is conducted in EtOH for 12 h at 40° C. 5-110 NaOH is used instead of KOH. The reaction is conducted for 7 h at 60° C. 5-111 NaOH is used instead of KOH. The reaction is conducted for 1 h at 70° C. 5-112 NaOH is used instead of KOH. The reaction is conducted for 20 h at 50° C. 5-113 NaOH is used instead of KOH. The reaction is conducted in EtOH for 12 h at 40° C. 5-114 NaOH is used instead of KOH. The reaction is conducted for 2.5 h at 60° C. 5-115 NaOH is used instead of KOH. The reaction is conducted for 2 h at 50° C. 5-116 NaOH is used instead of KOH. The reaction is conducted for 1.5 h at 70° C. 5-117 NaOH is used instead of KOH. The reaction is conducted in THF/EtOH for 2 h at 70° C. 5-118 NaOH is used instead of KOH. The reaction is conducted for 1 h at 70° C. 5-119 NaOH is used instead of KOH. The reaction is conducted for 1.5 h at 70° C. 5-120 NaOH is used instead of KOH. The reaction is conducted in EtOH for 4 h at rt. 5-121 LiOH is used instead of KOH. The reaction is conducted in THF/water for 12 h at rt. 5-122 The reaction is conducted for 48 h at 50° C. 5-123 NaOH is used instead of KOH. The reaction is conducted in MeOH for 12 h at rt. 5-124 NaOH is used instead of KOH. The reaction is conducted for 1.5 h at 50° C. 5-125 NaOH is used instead of KOH. The reaction is conducted in EtOH for 12 h at 50° C. 5-126 The reaction is conducted for 2 h at 50° C. 5-127 NaOH is used instead of KOH. The reaction is conducted for 2 days at 70° C. 5-128 NaOH is used instead of KOH. The reaction is conducted for 5 days at 70° C. 5-129 NaOH is used instead of KOH. The reaction is conducted for 35 h at 70° C. and for 3 days at rt. 5-130 NaOH is used instead of KOH. The reaction is conducted for 24 h at 70° C. and for 2 days at 50° C. 5-131 NaOH is used instead of KOH. The reaction is conducted for h at rt. 5-132 NaOH is used instead of KOH. The reaction is conducted for 45 min at rt. 5-133 NaOH is used instead of KOH. The reaction is conducted for 45 min at rt. 5-134 NaOH is used instead of KOH. The reaction is conducted for 4 h at rt. 5-135 NaOH is used instead of KOH. The reaction is conducted for 30 min at rt. 5-136 NaOH is used instead of KOH. The reaction is conducted for 1.5 h at rt. 5-137 NaOH is used instead of KOH. The reaction is conducted for 2.5 h at rt. 5-138 NaOH is used instead of KOH. The reaction is conducted for 45 min at rt. 5-139 NaOH is used instead of KOH. The reaction is conducted for 1.5 h at rt. 5-140 NaOH is used instead of KOH. The reaction is conducted for 16 h at rt. 5-141 NaOH is used instead of KOH. The reaction is conducted for 16 h at rt. 5-142 NaOH is used instead of KOH. The reaction is conducted for 72 h at rt. 5-143 NaOH is used instead of KOH. The reaction is conducted for 18 h at rt. 5-144 NaOH is used instead of KOH. The reaction is conducted for 2 h at 50° C. 5-145 NaOH is used instead of KOH. The reaction is conducted for 30 min at 50° C. 5-146 NaOH is used instead of KOH. The reaction is conducted in MeOH for 5 h at rt. 5-147 NaOH is used instead of KOH. The reaction is conducted for 12 h at 80° C. 5-148 NaOH is used instead of KOH. The reaction is conducted for 17 h at rt. 5-149 NaOH is used instead of KOH. The reaction is conducted for 17 h at rt. 5-150 NaOH is used instead of KOH. The reaction is conducted in EtOH for 12 h at rt. 5-151 NaOH is used instead of KOH. The reaction is conducted for 1.5 h at 40° C. 5-152 NaOH is used instead of KOH. The reaction is conducted for 3 h at 40° C. 5-153 NaOH is used instead of KOH. The reaction is conducted for 45 minutes at 40° C. 5-154 NaOH is used instead of KOH. The reaction is conducted in MeOH for 12 h at rt. 5-155 NaOH is used instead of KOH. The reaction is conducted in EtOH for 5 days at rt. 5-156 NaOH is used instead of KOH. The reaction is conducted in THF for 16 h at rt. 5-157 NaOH is used instead of KOH. The reaction is conducted in MeOH for 1 h at 40° C. 5-158 NaOH is used instead of KOH. The reaction is conducted in EtOH for 2 h at rt. 5-159 NaOH is used instead of KOH. The reaction is conducted in MeOH for 1 h at 40° C. 5-160 NaOH is used instead of KOH. The reaction is conducted in 1,4-dioxane/water 2:1 for 3 h at rt. 5-161 NaOH is used instead of KOH. The reaction is conducted in MeOH/dioxane for 4 h at 50° C. The product is purified by HPLC on reversed phase (ACN, water). 5-162 LiOH is used instead of KOH. The reaction is conducted in MeOH for 6 h at 75° C. 5-163 LiOH is used instead of KOH. The reaction is conducted in MeOH for 16 h at 75° C. 5-164 NaOH is used instead of KOH. The reaction is conducted at 70° C. for 1 h. 5-165 NaOH is used instead of KOH. The reaction is conducted at 70° C. for 2 h. 5-166 NaOH is used instead of KOH. The reaction is conducted at 70° C. for 1.5 h. 5-167 NaOH is used instead of KOH. The reaction is conducted at 70° C. for 1.5 h. 5-168 NaOH is used instead of KOH. The reaction is conducted in EtOH at r.t. for 3.5 h. 5-169 NaOH is used instead of KOH. The reaction is conducted at 70° C. for 1 h. 5-170 NaOH is used instead of KOH. The reaction is conducted at 50° C. for 2 h.

TABLE-US-00021 Intermediate Name Name of Starting Material 5-1 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- yl}-2-(methoxymethyl)pyridin-3-yl)methyl]-1H- hexan-3-yl}-2-(methoxymethyl)pyridin-3- pyrazole-4-carboxylic acid yl)methyl]-1H-pyrazole-4-carboxylate 5-2 1-[(2-Chloro-6-{6,6-difluoro-3- Ethyl 1-[(2-chloro-6-{6,6-difluoro-3- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- yl)methyl]-1H-1,2,3-triazole-4-carboxylic acid yl)methyl]-1H-1,2,3-triazole-4-carboxylate 5-3 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- Ethyl 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- (difluoromethyl)pyridin-3-yl)methyl]-1H-1,2,3- (difluoromethyl)pyridin-3-yl)methyl]-1H-1,2,3- triazole-4-carboxylic acid triazole-4-carboxylate 5-4 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- chloropyridin-3-yl)methyl]-1H-1,2,3-triazole-4- chloropyridin-3-yl)methyl]-1H-1,2,3-triazole-4- carboxylic acid carboxylate 5-5 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- bromopyridin-3-yl)methyl]-1H-1,2,3-triazole-4- bromopyridin-3-yl)methyl]-1H-1,2,3-triazole-4- carboxylic acid carboxylate 5-6 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 1-[(6-{6,6-difluoro-3- yl}-2-fluoropyridin-3-yl)methyl]-1H-imidazole- azabicyclo[3.1.0]hexan-3-yl}-2-fluoropyridin-3- 4-carboxylic acid yl)methyl]-1H-imidazole-4-carboxylate 5-7 1-[(5-{3-Azabicyclo[3.1.0]hexan-3-yl}pyridin-2- Ethyl 1-[(5-{3-azabicyclo[3.1.0]hexan-3-yl}- yl)methyl]-1H-1,2,3-triazole-4-carboxylic acid pyridin-2-yl)methyl]-1H-1,2,3-triazole-4- carboxylate 5-8 1-[(5-{3-Azabicyclo[3.1.0]hexan-3-yl}pyridin-2- Ethyl 1-[(5-{3-azabicyclo[3.1.0]hexan-3-yl}- yl)methyl]-1H-pyrazole-4-carboxylic acid pyridin-2-yl)methyl]-1H-pyrazole-4-carboxylate 5-9 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- fluoropyridin-3-yl)methyl]-1H-1,2,3-triazole-4- fluoropyridin-3-yl)methyl]-1H-1,2,3-triazole-4- carboxylic acid carboxylate 5-10 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- fluoropyridin-3-yl)methyl]-1H-imidazole-4- fluoropyridin-3-yl)methyl]-1H-imidazole-4- carboxylic acid carboxylate 5-11 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- fluoropyridin-3-yl)methyl]-1H-pyrazole-4- fluoropyridin-3-yl)methyl]-1H-pyrazole-4- carboxylic acid carboxylate 5-12 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- bromopyridin-3-yl)methyl]-1H-pyrazole-4- bromopyridin-3-yl)methyl]-1H-pyrazole-4- carboxylic acid carboxylate 5-13 1-[(2-Bromo-6-{6,6-difluoro-3- Ethyl 1-[(2-bromo-6-{6,6-difluoro-3- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- yl)methyl]-1H-1,2,3-triazole-4-carboxylic acid yl)methyl]-1H-1,2,3-triazole-4-carboxylate 5-14 1-[(2-Bromo-6-{6,6-difluoro-3- Ethyl 1-[(2-bromo-6-{6,6-difluoro-3- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- yl)methyl]-1H-pyrazole-4-carboxylic acid yl)methyl]-1H-pyrazole-4-carboxylate 5-15 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- bromopyridin-3-yl)methyl]-1H-imidazole-4- bromopyridin-3-yl)methyl]-1H-imidazole-4- carboxylic acid carboxylate 5-16 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- fluoropyridin-3-yl)methyl]-1H-1,2,3-triazole-4- fluoropyridin-3-yl)methyl]-1H-1,2,3-triazole-4- carboxylic acid carboxylate 5-17 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 1-[(6-{6,6-difluoro-3- yl}-2-fluoropyridin-3-yl)methyl]-1H-pyrazole-4- azabicyclo[3.1.0]hexan-3-yl}-2-fluoropyridin-3- carboxylic acid yl)methyl]-1H-pyrazole-4-carboxylate 5-18 1-[(5-{5-Azaspiro[2.3]hexan-5-yl}pyridin-2- Ethyl 1-[(5-{5-azaspiro[2.3]hexan-5-yl}pyridin- yl)methyl]-1H-pyrazole-4-carboxylic acid 2-yl)methyl]-1H-pyrazole-4-carboxylate 5-19 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- ethylpyridin-3-yl)methyl]-1H-1,2,3-triazole-4- ethylpyridin-3-yl)methyl]-1H-1,2,3-triazole-4- carboxylic acid carboxylate 5-20 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- chloropyridin-3-yl)methyl]-1H-imidazole-4- chloropyridin-3-yl)methyl]-1H-imidazole-4- carboxylic acid carboxylate 5-21 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- ethylpyridin-3-yl)methyl]-1H-pyrazole-4- ethylpyridin-3-yl)methyl]-1H-pyrazole-4- carboxylic acid carboxylate 5-22 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 1-[(6-{6,6-difluoro-3- yl}-2-fluoropyridin-3-yl)methyl]-1H-1,2,3- azabicyclo[3.1.0]hexan-3-yl}-2-fluoropyridin-3- triazole-4-carboxylic acid yl)methyl]-1H-1,2,3-triazole-4-carboxylate 5-23 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}pyridin-3- Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}pyridin- yl)methyl]-1H-1,2,3-triazole-4-carboxylic acid 3-yl)methyl]-1H-1,2,3-triazole-4-carboxylate 5-24 1-[(2-Bromo-6-{6,6-difluoro-3- Ethyl 1-[(2-bromo-6-{6,6-difluoro-3- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- yl)methyl]-1H-imidazole-4-carboxylic acid yl)methyl]-1H-imidazole-4-carboxylate 5-25 1-[(2-{3-Azabicyclo[3.1.0]hexan-3-yl}-4- Ethyl 1-[(2-{3-azabicyclo[3.1.0]hexan-3-yl}- (difluoromethyl)pyrimidin-5-yl)methyl]-1H- 4(difluoromethyl)pyrimidin-5-yl)methyl]-1H- imidazole-4-carboxylic acid imidazole-4-carboxylate 5-26 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- (difluoromethyl)pyridin-3-yl)methyl]-1H-1,2,3- (difluoromethyl)pyridin-3-yl)methyl]-1H-1,2,3- triazole-4-carboxylic acid triazole-4-carboxylate 5-27 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- propylpyridin-3-yl)methyl]-1H-imidazole-4- propylpyridin-3-yl)methyl]-1H-imidazole-4- carboxylic acid carboxylate 5-28 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- chloropyridin-3-yl)methyl]-1H-1,2,3-triazole-4- chloropyridin-3-yl)methyl]-1H-1,2,3-triazole-4- carboxylic acid carboxylate 5-29 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 1-[(6-{6,6-difluoro-3- yl}pyridin-3-yl)methyl]-1H-1,2,3-triazole-4- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- carboxylic acid yl)methyl]-1H-1,2,3-triazole-4-carboxylate 5-30 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- methylpyridin-3-yl)methyl]-1H-imidazole-4- methylpyridin-3-yl)methyl]-1H-imidazole-4- carboxylic acid carboxylate 5-31 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 1-[(6-{6,6-difluoro-3- yl}-2-methylpyridin-3-yl)methyl]-1H-imidazole- azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin- 4-carboxylic acid 3-yl)methyl]-1H-imidazole-4-carboxylate 5-32 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- bromopyridin-3-yl)methyl]-1H-1,2,3-triazole-4- bromopyridin-3-yl)methyl]-1H-1,2,3-triazole-4- carboxylic acid carboxylate 5-33 1-[(5-{3-Azabicyclo[3.1.0]hexan-3-yl}-6- Ethyl 1-[(5-{3-azabicyclo[3.1.0]hexan-3-yl}-6- cyanopyridin-2-yl)methyl]-1H-pyrazole-4- cyanopyridin-2-yl)methyl]-1H-pyrazole-4- carboxylic acid carboxylate 5-34 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 1-[(6-{6,6-difluoro-3- yl}-2-ethylpyridin-3-yl)methyl]-1H-pyrazole-4- azabicyclo[3.1.0]hexan-3-yl}-2-ethylpyridin-3- carboxylic acid yl)methyl]-1H-pyrazole-4-carboxylate 5-35 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- ethylpyridin-3-yl)methyl]-1H-imidazole-4- ethylpyridin-3-yl)methyl]-1H-imidazole-4- carboxylic acid carboxylate 5-36 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- (trifluoromethoxy)pyridin-3-yl)methyl]-1H- (trifluoromethoxy)pyridin-3-yl)methyl]-1H- imidazole-4-carboxylic acid imidazole-4-carboxylate 5-37 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- bromopyridin-3-yl)methyl]-1H-imidazole-4- bromopyridin-3-yl)methyl]-1H-imidazole-4- carboxylic acid carboxylate 5-38 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- bromopyridin-3-yl)methyl]-1H-pyrazole-4- bromopyridin-3-yl)methyl]-1H-pyrazole-4- carboxylic acid carboxylate 5-39 1-[(5-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 1-[(5-{6,6-difluoro-3- yl}pyridin-2-yl)methyl]-1H-pyrazole-4- azabicyclo[3.1.0]hexan-3-yl}pyridin-2- carboxylic acid yl)methyl]-1H-pyrazole-4-carboxylate 5-40 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(1- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- cyanocyclopropyl)pyridin-3-yl)methyl]-1H- (1-cyanocyclopropyl)pyridin-3-yl)methyl]-1H- pyrazole-4-carboxylic acid pyrazole-4-carboxylate 5-41 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- (difluoromethyl)pyridin-3-yl)methyl]-1H- (difluoromethyl)pyridin-3-yl)methyl]-1H- pyrazole-4-carboxylic acid pyrazole-4-carboxylate 5-42 1-[(2-Chloro-6-{6,6-difluoro-3- Ethyl 1-[(2-chloro-6-{6,6-difluoro-3- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- yl)methyl]-1H-pyrazole-4-carboxylic acid yl)methyl]-1H-pyrazole-4-carboxylate 5-43 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- yl}-2-(difluoromethyl)pyridin-3-yl)methyl]-1H- hexan-3-yl}-2-(difluoromethyl)pyridin-3- imidazole-4-carboxylic acid yl)methyl]-1H-imidazole-4-carboxylate 5-44 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- yl}-2-(difluoromethyl)pyridin-3-yl)methyl]-1H- hexan-3-yl}-2-(difluoromethyl)pyridin-3- pyrazole-4-carboxylic acid yl)methyl]-1H-pyrazole-4-carboxylate 5-45 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- (difluoromethyl)pyridin-3-yl)methyl]-1H- (difluoromethyl)pyridin-3-yl)methyl]-1H- imidazole-4-carboxylic acid imidazole-4-carboxylate 5-46 1-[(2-{3-Azabicyclo[3.1.0]hexan-3-yl}-4- Ethyl 1-[(2-{3-azabicyclo[3.1.0]hexan-3-yl}-4- (difluoromethyl)pyrimidin-5-yl)methyl]-1H- (difluoromethyl)pyrimidin-5-yl)methyl]-1H- pyrazole-4-carboxylic acid pyrazole-4-carboxylate 5-47 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- ethylpyridin-3-yl)methyl]-1H-pyrazole-4- ethylpyridin-3-yl)methyl]-1H-pyrazole-4- carboxylic acid carboxylate 5-48 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- (difluoromethyl)pyridin-3-yl)methyl]-1H- (difluoromethyl)pyridin-3-yl)methyl]-1H- pyrazole-4-carboxylic acid pyrazole-4-carboxylate 5-49 1-[(5-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 1-[(5-{6,6-difluoro-3- yl}pyridin-2-yl)methyl]-1H-imidazole-4- azabicyclo[3.1.0]hexan-3-yl}pyridin-2- carboxylic acid yl)methyl]-1H-imidazole-4-carboxylate 5-50 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(3- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- hydroxypropyl)pyridin-3-yl)methyl]-1H- (3-hydroxypropyl)pyridin-3-yl)methyl]-1H- pyrazole-4-carboxylic acid pyrazole-4-carboxylate 5-51 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- chloropyridin-3-yl)methyl]-1H-pyrazole-4- chloropyridin-3-yl)methyl]-1H-pyrazole-4- carboxylic acid carboxylate 5-52 1-[(2-Chloro-6-{6,6-difluoro-3- Ethyl 1-[(2-chloro-6-{6,6-difluoro-3- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- yl)methyl]-1H-imidazole-4-carboxylic acid yl)methyl]-1H-imidazole-4-carboxylate 5-53 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- (trifluoromethoxy)pyridin-3-yl)methyl]-1H- (trifluoromethoxy)pyridin-3-yl)methyl]-1H- pyrazole-4-carboxylic acid pyrazole-4-carboxylate 5-54 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(1- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- hydroxypropan-2-yl)pyridin-3-yl)methyl]-1H- (1-hydroxypropan-2-yl)pyridin-3-yl)methyl]-1H- pyrazole-4-carboxylic acid pyrazole-4-carboxylate 5-55 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}pyridazin- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}- 3-yl)methyl]-1H-pyrazole-4-carboxylic acid pyridazin-3-yl)methyl]-1H-pyrazole-4- carboxylate 5-56 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- (hydroxymethyl)pyridin-3-yl)methyl]-1H-1,2,3- (hydroxymethyl)pyridin-3-yl)methyl]-1H-1,2,3- triazole-4-carboxylic acid triazole-4-carboxylate 5-57 1-[(2-{3-Azabicyclo[3.1.0]hexan-3-yl}-4- Ethyl 1-[(2-{3-azabicyclo[3.1.0]hexan-3-yl}-4- (hydroxymethyl)pyrimidin-5-yl)methyl]-1H- (hydroxymethyl)pyrimidin-5-yl)methyl]-1H- pyrazole-4-carboxylic acid pyrazole-4-carboxylate 5-58 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 1-[(6-{6,6-difluoro-3- yl}pyridin-3-yl)methyl]-1H-imidazole-4- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- carboxylic acid yl)methyl]-1H-imidazole-4-carboxylate 5-59 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- (difluoromethyl)pyridin-3-yl)methyl]-1H- (difluoromethyl)pyridin-3-yl)methyl]-1H- imidazole-4-carboxylic acid imidazole-4-carboxylate 5-60 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(1- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- cyano-1-methylethyl)pyridin-3-yl)methyl]-1H- (1-cyano-1-methylethyl)pyridin-3-yl)methyl]- pyrazole-4-carboxylic acid 1H-pyrazole-4-carboxylate 5-61 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 1-[(6-{6,6-difluoro-3- yl}-2-propylpyridin-3-yl)methyl]-1H-imidazole- azabicyclo[3.1.0]hexan-3-yl}-2-propylpyridin-3- 4-carboxylic acid yl)methyl]-1H-imidazole-4-carboxylate 5-62 1-[(2-Cyclobutyl-6-{6,6-difluoro-3- Ethyl 1-[(2-cyclobutyl-6-{6,6-difluoro-3- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- yl)methyl]-1H-pyrazole-4-carboxylic acid yl)methyl]-1H-pyrazole-4-carboxylate 5-63 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- methylpyridin-3-yl)methyl]-1H-1,2,3-triazole-4- methylpyridin-3-yl)methyl]-1H-1,2,3-triazole-4- carboxylic acid carboxylate 5-64 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 1-[(6-{6,6-difluoro-3- yl}-2-propylpyridin-3-yl)methyl]-1H-pyrazole-4- azabicyclo[3.1.0]hexan-3-yl}-2-propylpyridin-3- carboxylic acid yl)methyl]-1H-pyrazole-4-carboxylate 5-65 1-[(5-{3-Azabicyclo[3.1.0]hexan-3-yl}pyrimidin- Ethyl 1-[(5-{3-azabicyclo[3.1.0]hexan-3-yl}- 2-yl)methyl]-1H-pyrazole-4-carboxylic acid pyrimidin-2-yl)methyl]-1H-pyrazole-4- carboxylate 5-66 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}pyridin-3- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}- yl)methyl]-1H-imidazole-4-carboxylic acid pyridin-3-yl)methyl]-1H-imidazole-4- carboxylate 5-67 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- ethylpyridin-3-yl)methyl]-1H-imidazole-4- ethylpyridin-3-yl)methyl]-1H-imidazole-4- carboxylic acid carboxylate 5-68 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 1-[(6-{6,6-difluoro-3- yl}-2-ethylpyridin-3-yl)methyl]-1H-imidazole-4- azabicyclo[3.1.0]hexan-3-yl}-2-ethylpyridin-3- carboxylic acid yl)methyl]-1H-imidazole-4-carboxylate 5-69 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- (hydroxymethyl)pyridin-3-yl)methyl]-1H- (hydroxymethyl)pyridin-3-yl)methyl]-1H- pyrazole-4-carboxylic acid pyrazole-4-carboxylatetrifluoroacetate 5-70 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- (cyanomethyl)pyridin-3-yl)methyl]-1H- (cyanomethyl)pyridin-3-yl)methyl]-1H- pyrazole-4-carboxylic acid pyrazole-4-carboxylate 5-71 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(1- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- hydroxyethyl)pyridin-3-yl)methyl]-1H-pyrazole- (1-hydroxyethyl)pyridin-3-yl)methyl]-1H- 4-carboxylic acid pyrazole-4-carboxylate 5-72 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-[(1R)- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- 1-hydroxyethyl]pyridin-3-yl)methyl]-1H- [(1R)-1-hydroxyethyl]pyridin-3-yl)methyl]-1H- pyrazole-4-carboxylic acid pyrazole-4-carboxylate 5-73 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-[(1S)- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- 1-hydroxyethyl]pyridin-3-yl)methyl]-1H- [(1R)-1-hydroxyethyl]pyridin-3-yl)methyl]-1H- pyrazole-4-carboxylic acid pyrazole-4-carboxylate 5-74 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- hydroxypropan-2-yl)pyridin-3-yl)methyl]-1H- (2-hydroxypropan-2-yl)pyridin-3-yl)methyl]-1H- pyrazole-4-carboxylic acid pyrazole-4-carboxylate 5-75 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- hydroxyethyl)pyridin-3-yl)methyl]-1H-pyrazole- (2-hydroxyethyl)pyridin-3-yl)methyl]-1H- 4-carboxylic acid pyrazole-4-carboxylate 5-76 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- (hydroxymethyl)pyridin-3-yl)methyl]-1H- (hydroxymethyl)pyridin-3-yl)methyl]-1H- pyrazole-4-carboxylic acid pyrazole-4-carboxylate 5-77 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- (hydroxymethyl)pyridin-3-yl)methyl]-1H- (hydroxymethyl)pyridin-3-yl)methyl]-1H- imidazole-4-carboxylic acid imidazole-4-carboxylate 5-78 1-[(5-{3-Azabicyclo[3.1.0]hexan-3-yl}-3- Ethyl 1-[(5-{3-azabicyclo[3.1.0]hexan-3-yl}-3- (hydroxymethyl)pyrazin-2-yl)methyl]-1H- (hydroxymethyl)pyrazin-2-yl)methyl]-1H- pyrazole-4-carboxylic acid pyrazole-4-carboxylate 5-79 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 1-[(6-{6,6-difluoro-3- yl}-2-methylpyridin-3-yl)methyl]-1H-pyrazole- azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin- 4-carboxylic acid 3-yl)methyl]-1H-pyrazole-4-carboxylate 5-80 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- (hydroxymethyl)pyridin-3-yl)methyl]-1H-1,2,3- (hydroxymethyl)pyridin-3-yl)methyl]-1H-1,2,3- triazole-4-carboxylic acid triazole-4-carboxylate 5-81 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- yl}-2-(hydroxymethyl)pyridin-3-yl)methyl]-1H- hexan-3-yl}-2-(hydroxymethyl)pyridin-3-yl)- pyrazole-4-carboxylic acid methyl]-1H-pyrazole-4-carboxylate 5-82 1-[(5-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 1-[(5-{6,6-difluoro-3- yl}-3-methylpyrazin-2-yl)methyl]-1H-1,2,3- azabicyclo[3.1.0]hexan-3-yl}-3-methylpyrazin- triazole-4-carboxylic acid 2-yl)methyl]-1H-1,2,3-triazole-4-carboxylate 5-83 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- yl}-2-(methoxymethyl)pyridin-3-yl)methyl]-1H- hexan-3-yl}-2-(methoxymethyl)pyridin-3- imidazole-4-carboxylic acid yl)methyl]-1H-imidazole-4-carboxylate 5-84 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- yl}-2-(hydroxymethyl)pyridin-3-yl)methyl]-1H- hexan-3-yl}-2-(hydroxymethyl)pyridin-3- imidazole-4-carboxylic acid yl)methyl]-1H-imidazole-4-carboxylate 5-85 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- yl}-2-(hydroxymethyl)pyridin-3-yl)methyl]-1H- hexan-3-yl}-2-(hydroxymethyl)pyridin-3- 1,2,3-triazole-4-carboxylic acid yl)methyl]-1H-1,2,3-triazole-4-carboxylate 5-86 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Methyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- methylpyridin-3-yl)methyl]-3-cyano-1H- methylpyridin-3-yl)methyl]-3-cyano-1H- pyrazole-4-carboxylic acid pyrazole-4-carboxylate 5-87 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-4- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-4- chloropyridin-3-yl)methyl]-1H-pyrazole-4- chloropyridin-3-yl)methyl]-1H-pyrazole-4- carboxylic acid carboxylate 5-88 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- methylpropyl)pyridin-3-yl)methyl]-1H- (2-methylpropyl)pyridin-3-yl)methyl]-1H- imidazole-4-carboxylic acid imidazole-4-carboxylate 5-89 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- ethylpyridin-3-yl)methyl]-1H-1,2,3-triazole-4- ethylpyridin-3-yl)methyl]-1H-1,2,3-triazole-4- carboxylic acid carboxylate 5-90 1-[(5-Cyano-6-{6,6-difluoro-3- Ethyl 1-[(5-cyano-6-{6,6-difluoro-3- azabicyclo[3.1.0]hexan-3-yl}-4-methylpyridin- azabicyclo[3.1.0]hexan-3-yl}-4-methylpyridin- 3-yl)methyl]-1H-pyrazole-4-carboxylic acid 3-yl)methyl]-1H-pyrazole-4-carboxylate 5-91 1-[(5-Cyano-6-{6,6-difluoro-3- Ethyl 1-[(5-cyano-6-{6,6-difluoro-3- azabicyclo[3.1.0]hexan-3-yl}-4-methylpyridin- azabicyclo[3.1.0]hexan-3-yl}-4-methylpyridin- 3-yl)methyl]-1H-imidazole-4-carboxylic acid 3-yl)methyl]-1H-imidazole-4-carboxylate 5-92 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- fluoropyridin-3-yl)methyl]-1H-pyrazole-4- fluoropyridin-3-yl)methyl]-1H-pyrazole-4- carboxylic acid carboxylate 5-93 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-4- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-4- methylpyridin-3-yl)methyl]-1H-imidazole-4- methylpyridin-3-yl)methyl]-1H-imidazole-4- carboxylic acid carboxylate 5-94 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 3-(chloromethyl)-1-[(6-{6,6-difluoro-3- yl}-2-methylpyridin-3-yl)methyl]-3- azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin- (hydroxymethyl)-1H-pyrazole-4-carboxylic acid 3-yl)methyl]-1H-pyrazole-4-carboxylate 5-95 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- methylpyridin-3-yl)methyl]-3-(cyanomethyl)- methylpyridin-3-yl)methyl]-3-(cyanomethyl)- 1H-pyrazole-4-carboxylic acid 1H-pyrazole-4-carboxylate 5-96 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- fluoropyridin-3-yl)methyl]-1H-imidazole-4- fluoropyridin-3-yl)methyl]-1H-imidazole-4- carboxylic acid carboxylate 5-97 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 1-[(6-{6,6-difluoro-3- yl}-2-ethylpyridin-3-yl)methyl]-1H-1,2,3- azabicyclo[3.1.0]hexan-3-yl}-2-ethylpyridin-3- triazole-4-carboxylic acid yl)methyl]-1H-1,2,3-triazole-4-carboxylate 5-98 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- propylpyridin-3-yl)methyl]-1H-imidazole-4- propylpyridin-3-yl)methyl]-1H-imidazole-4- carboxylic acid carboxylate 5-99 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- yl}-2,4-dimethylpyridin-3-yl)methyl]-1H- hexan-3-yl}-2,4-dimethylpyridin-3-yl)methyl]- imidazole-4-carboxylic acid 1H-imidazole-4-carboxylate 5-100 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- yl}-2,4-dimethylpyridin-3-yl)methyl]-1H- hexan-3-yl}-2,4-dimethylpyridin-3-yl)methyl]- pyrazole-4-carboxylic acid 1H-pyrazole-4-carboxylate 5-101 5-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 5-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- yl}-2-methylpyridin-3-yl)methyl]thiophene-2- hexan-3-yl}-2-methylpyridin-3-yl)- carboxylic acid methyl]thiophene-2-carboxylate 5-102 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-5- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-5- cyanopyridin-3-yl)methyl]-1H-pyrazole-4- cyanopyridin-3-yl)methyl]-1H-pyrazole-4- carboxylic acid carboxylate 5-103 2-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Methyl 2-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- yl}-2-methylpyridin-3-yl)methyl]-1,3-thiazole-5- hexan-3-yl}-2-methylpyridin-3-yl)methyl]-1,3- carboxylic acid thiazole-5-carboxylate 5-104 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-5-cyano- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-5- 4-methylpyridin-3-yl)methyl]-1H-pyrazole-4- cyano-4-methylpyridin-3-yl)methyl]-1H- carboxylic acid pyrazole-4-carboxylate 5-105 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- cyclopropylpyridin-3-yl)methyl]-1H-pyrazole-4- cyclopropylpyridin-3-yl)methyl]-1H-pyrazole-4- carboxylic acid carboxylate 5-106 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-5-cyano- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-5- 4-methylpyridin-3-yl)methyl]-1H-imidazole-4- cyano-4-methylpyridin-3-yl)methyl]-1H- carboxylic acid imidazole-4-carboxylate 5-107 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-4- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-4- chloropyridin-3-yl)methyl]-1H-imidazole-4- chloropyridin-3-yl)methyl]-1H-imidazole-4- carboxylic acid carboxylate 5-108 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-4- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-4- (trifluoromethyl)pyridin-3-yl)methyl]-1H- (trifluoromethyl)pyridin-3-yl)methyl]-1H- imidazole-4-carboxylic acid imidazole-4-carboxylate 5-109 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- chloropyridin-3-yl)methyl]-1H-imidazole-4- chloropyridin-3-yl)methyl]-1H-imidazole-4- carboxylic acid carboxylate 5-110 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- methylpyridin-3-yl)methyl]-3-(methoxymethyl)- methylpyridin-3-yl)methyl]-3-(methoxymethyl)- 1H-pyrazole-4-carboxylic acid 1H-pyrazole-4-carboxylate 5-111 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-5-cyano- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-5- 2-methylpyridin-3-yl)methyl]-1H-imidazole-4- cyano-2-methylpyridin-3-yl)methyl]-1H- carboxylic acid imidazole-4-carboxylate 5-112 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- yl}-2-methylpyridin-3-yl)methyl]-3-(methoxy- hexan-3-yl}-2-methylpyridin-3-yl)methyl]-3- methyl)-1H-pyrazole-4-carboxylic acid (methoxymethyl)-1H-pyrazole-4-carboxylate 5-113 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- chloropyridin-3-yl)methyl]-1H-pyrazole-4- chloropyridin-3-yl)methyl]-1H-pyrazole-4- carboxylic acid carboxylate 5-114 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- methylpyridin-3-yl)methyl]-3-bromo-1H- methylpyridin-3-yl)methyl]-3-bromo-1H- pyrazole-4-carboxylic acid pyrazole-4-carboxylate 5-115 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-5- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-5- bromo-2-methylpyridin-3-yl)methyl]-1H- bromo-2-methylpyridin-3-yl)methyl]-1H- pyrazole-4-carboxylic acid pyrazole-4-carboxylate 5-116 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-5-cyano- Methyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-5- 2-methylpyridin-3-yl)methyl]-1H-pyrazole-4- cyano-2-methylpyridin-3-yl)methyl]-1H- carboxylic acid pyrazole-4-carboxylate 5-117 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-5- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-5- bromo-2-methylpyridin-3-yl)methyl]-1H- bromo-2-methylpyridin-3-yl)methyl]-1H- imidazole-4-carboxylic acid imidazole-4-carboxylate 5-118 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2,5- Methyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}- dimethylpyridin-3-yl)methyl]-1H-imidazole-4- 2,5-dimethylpyridin-3-yl)methyl]-1H-imidazole- carboxylic acid 4-carboxylate 5-119 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2,5- Methyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}- dimethylpyridin-3-yl)methyl]-1H-pyrazole-4- 2,5-dimethylpyridin-3-yl)methyl]-1H-pyrazole- carboxylic acid 4-carboxylate 5-120 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- cyanopyridin-3-yl)methyl]-1H-pyrazole-4- cyanopyridin-3-yl)methyl]-1H-pyrazole-4- carboxylic acid carboxylate 5-121 1-[(2-Chloro-4-methylpyrimidin-5-yl)methyl]- Ethyl 1-[(2-chloro-4-methylpyrimidin-5-yl)- 1H-pyrazole-4-carboxylic acid methyl]-1H-pyrazole-4-carboxylate 5-122 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- methylpyridin-3-yl)methyl]-1H-pyrazole-4- methylpyridin-3-yl)methyl]-1H-pyrazole-4- carboxylic acid carboxylate 5-123 1-[(2-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 1-[(2-{6,6-difluoro-3-azabicyclo[3.1.0]- yl}-4-methylpyrimidin-5-yl)methyl]-1H- hexan-3-yl}-4-methylpyrimidin-5-yl)methyl]- imidazole-4-carboxylic acid 1H-imidazole-4-carboxylate 5-124 1-[(2-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 1-[(2-{6,6-difluoro-3-azabicyclo[3.1.0]- yl}-4-methylpyrimidin-5-yl)methyl]-1H-1,2,3- hexan-3-yl}-4-methylpyrimidin-5-yl)methyl]- triazole-4-carboxylic acid 1H-1,2,3-triazole-4-carboxylate 5-125 1-[(2-{3-Azabicyclo[3.1.0]hexan-3-yl}-4- Ethyl 1-[(2-{3-azabicyclo[3.1.0]hexan-3-yl}-4- methylpyrimidin-5-yl)methyl]-1H-1,2,3-triazole- methylpyrimidin-5-yl)methyl]-1H-1,2,3-triazole- 4-carboxylic acid 4-carboxylate 5-126 1-[(5-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 1-[(5-{6,6-difluoro-3-azabicyclo[3.1.0]- yl}pyridin-2-yl)methyl]-1H-1,2,3-triazole-4- hexan-3-yl}pyridin-2-yl)methyl]-1H-1,2,3- carboxylic acid triazole-4-carboxylate 5-127 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Methyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- yl}pyridin-3-yl)methyl]-1H-pyrrole-3-carboxylic hexan-3-yl}pyridin-3-yl)methyl]-1H-pyrrole-3- acid carboxylate 5-128 5-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Methyl 5-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- yl}pyridin-3-yl)methyl]-1H-pyrrole-3-carboxylic hexan-3-yl}pyridin-3-yl)methyl]-1H-pyrrole-3- acid carboxylate 5-129 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Methyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- yl}-2-methylpyridin-3-yl)methyl]-1H-pyrrole-3- hexan-3-yl}-2-methylpyridin-3-yl)methyl]-1H- carboxylic acid pyrrole-3-carboxylate 5-130 5-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Methyl 5-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- yl}-2-methylpyridin-3-yl)methyl]-1H-pyrrole-3- hexan-3-yl}-2-methylpyridin-3-yl)methyl]-1H- carboxylic acid pyrrole-3-carboxylate 5-131 1-({2-Ethyl-6-[(1R,5S,6R)-6-methyl-3- Ethyl 1-({2-ethyl-6-[(1R,5S,6R)-6-methyl-3- azabicyclo[3.1.0]hexan-3-yl]pyridin-3- azabicyclo[3.1.0]hexan-3-yl]pyridin-3- yl}methyl)-1H-imidazole-4-carboxylic acid yl}methyl)-1H-imidazole-4-carboxylate 5-132 3-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- (difluoromethyl)pyridin-3-yl)methyl]-1,2- (difluoromethyl)pyridin-3-yl)methyl]-1,2- oxazole-5-carboxylic acid oxazole-5-carboxylate 5-133 3-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 3-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- yl}-2-(difluoromethyl)pyridin-3-yl)methyl]-1,2- hexan-3-yl}-2-(difluoromethyl)pyridin-3- oxazole-5-carboxylic acid yl)methyl]-1,2-oxazole-5-carboxylate 5-134 3-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- ethylpyridin-3-yl)methyl]-1,2-oxazole-5- ethylpyridin-3-yl)methyl]-1,2-oxazole-5- carboxylic acid carboxylate 5-135 3-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 3-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- yl}-2-(hydroxymethyl)pyridin-3-yl)methyl]-1,2- hexan-3-yl}-2-(hydroxymethyl)pyridin-3- oxazole-5-carboxylic acid yl)methyl]-1,2-oxazole-5-carboxylate 5-136 3-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- chloropyridin-3-yl)methyl]-1,2-oxazole-5- chloropyridin-3-yl)methyl]-1,2-oxazole-5- carboxylic acid carboxylate 5-137 3-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- (hydroxymethyl)pyridin-3-yl)methyl]-1,2- (hydroxymethyl)pyridin-3-yl)methyl]-1,2- oxazole-5-carboxylic acid oxazole-5-carboxylate 5-138 3-[(2-Chloro-6-{6,6-difluoro-3- Ethyl 3-[(2-chloro-6-{6,6-difluoro-3- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- yl)methyl]-1,2-oxazole-5-carboxylic acid yl)methyl]-1,2-oxazole-5-carboxylate 5-139 3-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}pyridin-3- Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}- yl)methyl]-1,2-oxazole-5-carboxylic acid pyridin-3-yl)methyl]-1,2-oxazole-5-carboxylate 5-140 3-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 3-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- yl}-2-methylpyridin-3-yl)methyl]-1,2-oxazole-5- hexan-3-yl}-2-methylpyridin-3-yl)methyl]-1,2- carboxylic acid oxazole-5-carboxylate 5-141 2-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Methyl 2-[1-(6-{3-azabicyclo[3.1.0]hexan-3-yl}- methylpyridin-3-yl)methyl]-1,3-oxazole-5- 2-methylpyridin-3-yl)-2-methoxy-2-oxoethyl]- carboxylic acid 1,3-oxazole-5-carboxylate 5-142 3-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 3-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- yl}-2-ethylpyridin-3-yl)methyl]-1,2-oxazole-5- hexan-3-yl}-2-ethylpyridin-3-yl)methyl]-1,2- carboxylic acid oxazole-5-carboxylate 5-143 2-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 2-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- yl}-2-methylpyridin-3-yl)methyl]-1,3-oxazole-5- hexan-3-yl}-2-methylpyridin-3-yl)methyl]-1,3- carboxylic acid oxazole-5-carboxylate 5-144 3-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- Ethyl 3-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- chloropyridin-3-yl)methyl]-1,2-oxazole-5- chloropyridin-3-yl)methyl]-1,2-oxazole-5- carboxylic acid carboxylate 5-145 3-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- Ethyl 3-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- ethylpyridin-3-yl)methyl]-1,2-oxazole-5- ethylpyridin-3-yl)methyl]-1,2-oxazole-5- carboxylic acid carboxylate 5-146 2-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Methyl 2-[1-(6-{3-azabicyclo[3.1.0]hexan-3-yl}- ethylpyridin-3-yl)methyl]-1,3-oxazole-5- 2-ethylpyridin-3-yl)-2-methoxy-2-oxoethyl]- carboxylic acid 1,3-oxazole-5-carboxylate 5-147 5-[(2-Chloro-6-{6,6-difluoro-3- Methyl 5-[(2-chloro-6-{6,6-difluoro-3- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- yl)methyl]-1H-pyrrole-3-carboxylic acid yl)methyl]-1H-pyrrole-3-carboxylate 5-148 3-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 3-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- yl}-2-ethylpyridin-3-yl)methyl]-1-(propan-2-yl)- hexan-3-yl}-2-ethylpyridin-3-yl)methyl]-1- 1H-pyrazole-5-carboxylic acid (propan-2-yl)-1H-pyrazole-5-carboxylate 5-149 3-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 3-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- yl}-2-ethylpyridin-3-yl)methyl]-1-ethyl-1H- hexan-3-yl}-2-ethylpyridin-3-yl)methyl]-1-ethyl- pyrazole-5-carboxylic acid 1H-pyrazole-5-carboxylate 5-150 3-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- (hydroxymethyl)pyridin-3-yl)methyl]-1-{[2- (hydroxymethyl)pyridin-3-yl)methyl]-1-{[2- (trimethylsilyl)ethoxy]methyl}-1H-pyrazole-5- (trimethylsilyl)ethoxy]methyl}-1H-pyrazole-5- carboxylic acid and carboxylate and 5-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 5-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- (hydroxymethyl)pyridin-3-yl)methyl]-1-{[2- (hydroxymethyl)pyridin-3-yl)methyl]-1-{[2- (trimethylsilyl)ethoxy]methyl}-1H-pyrazole-3- (trimethylsilyl)ethoxy]methyl}-1H-pyrazole-3- carboxylic acid (mixture of isomers) carboxylate (mixture of isomers) 5-151 3-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- ethylpyridin-3-yl)methyl]-1-ethyl-1H-pyrazole- ethylpyridin-3-yl)methyl]-1-ethyl-1H-pyrazole- 5-carboxylic acid 5-carboxylate 5-152 3-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 3-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- yl}-2-methylpyridin-3-yl)methyl]-1-ethyl-1H- hexan-3-yl}-2-methylpyridin-3-yl)methyl]-1- pyrazole-5-carboxylic acid ethyl-1H-pyrazole-5-carboxylate 5-153 3-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- methylpyridin-3-yl)methyl]-1-ethyl-1H- methylpyridin-3-yl)methyl]-1-ethyl-1H- pyrazole-5-carboxylic acid pyrazole-5-carboxylate 5-154 3-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- ethylpyridin-3-yl)methyl]-1H-pyrazole-5- ethylpyridin-3-yl)methyl]-1H-pyrazole-5- carboxylic acid carboxylate 5-155 3-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- ethylpyridin-3-yl)methyl]-1-methyl-1H- ethylpyridin-3-yl)methyl]-1-methyl-1H- pyrazole-5-carboxylic acid pyrazole-5-carboxylate 5-156 3-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- methylpyridin-3-yl)methyl]-1-methyl-1H- methylpyridin-3-yl)methyl]-1-methyl-1H- pyrazole-5-carboxylic acid pyrazole-5-carboxylate 5-157 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- methylpyrimidin-3-yl)methyl]-1H-imidazole-4- methylpyrimidin-3-yl)methyl]-1H-imidazole-4- carboxylic acid carboxylate 5-158 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- methylpyridin-3-yl)methyl]-1H-imidazole-4- methylpyridin-3-yl)methyl]-1H-imidazole-4- carboxylic acid carboxylate 5-159 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- methylpyrimidin-3-yl)methyl]-1H-imidazole-4- methylpyrimidin-3-yl)methyl]-1H-imidazole-4- carboxylic acid carboxylate 5-160 2-chloro-1-[(6-{6,6-difluoro-3- Ethyl 2-chloro-1-[(6-{6,6-difluoro-3- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- yl)methyl]-1H-imidazole-4-carboxylic acid yl)methyl]-1H-imidazole-4-carboxylate 5-161 7-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Methyl 7-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- methylpyridin-3-yl)methyl]-7H-pyrrolo[2,3- methylpyridin-3-yl)methyl]-7H-pyrrolo[2,3- d]pyrimidine-5-carboxylic acid d]pyrimidine-5-carboxylate 5-162 7-[(2-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Methyl 7-[(2-{6,6-difluoro-3-azabicyclo[3.1.0]- yl}-4-methylpyrimidin-5-yl)methyl]-7H- hexan-3-yl}-4-methylpyrimidin-5-yl)methyl]- pyrrolo[2,3-d]pyrimidine-5-carboxylic acid 7H-pyrrolo[2,3-d]pyrimidine-5-carboxylate 5-163 7-[(2-{3-Azabicyclo[3.1.0]hexan-3-yl}-4- Methyl 7-[(2-{3-azabicyclo[3.1.0]hexan-3-yl}-4- methylpyrimidin-5-yl)methyl]-7H-pyrrolo[2,3- methylpyrimidin-5-yl)methyl]-7H-pyrrolo[2,3- d]pyrimidine-5-carboxylic acid d]pyrimidine-5-carboxylate 5-164 5-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Methyl 5-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- methylpyridin-3-yl)methyl]furan-2-carboxylic methylpyridin-3-yl)methyl]furan-2-carboxylate acid 5-165 5-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 5-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- methylpyridin-3-yl)methyl]furan-3-carboxylic methylpyridin-3-yl)methyl]furan-3-carboxylate acid 5-166 5-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Methyl 5-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- yl}-2-methylpyridin-3-yl)methyl]furan-2- hexan-3-yl}-2-methylpyridin-3-yl)methyl]furan- carboxylic acid 2-carboxylate 5-167 5-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Methyl 5-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- yl}-2-ethylpyridin-3-yl)methyl]furan-2- hexan-3-yl}-2-ethylpyridin-3-yl)methyl]furan-2- carboxylic acid carboxylate 5-168 5-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-ethyl- Ethyl 5-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- pyridin-3-yl)methyl]thiophene-2-carboxylic acid ethylpyridin-3-yl)methyl]thiophene-2- carboxylate 5-169 5-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 5-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- yl}-2-ethylpyridin-3-yl)methyl]thiophene-2- hexan-3-yl}-2-ethylpyridin-3- carboxylic acid yl)methyl]thiophene-2-carboxylate 5-170 5-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 5-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- yl}-2-(hydroxymethyl)pyridin-3- hexan-3-yl}-2-(hydroxymethyl)-pyridin-3- yl)methyl]thiophene-2-carboxylic acid yl)methyl]thiophene-2-carboxylate

Intermediate 6

2-Bromo-6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}pyridine-3-carbaldehyde

[0319] ##STR00309##

[0320] Under argon atmosphere a mixture of 2,6-dibromopyridine-3-carbaldehyde (5.0 g), 6,6-difluoro-3-azabicyclo[3.1.0]hexane hydrochloride (3.0 g) and DIPEA (8 mL) in DMF (50 mL) is stirred at 50° C. for 12 h. The mixture is cooled to rt, concentrated in vacuo, partitioned between water and EtOAc and the phases are separated. The organic phase is washed with brine, dried (MgSO.sub.4), concentrated and the residue is chromatographed on silica gel (petroleum ether/EtOAc 85:15.fwdarw.70:30) to give the title compound.

[0321] LC (Method 2): t.sub.R=1.03 min; Mass spectrum (ESI+): m/z=303 [M+H].sup.+.

[0322] Intermediates 6-1 to 6-19 are prepared in analogy to Intermediate 6:

TABLE-US-00022 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M + H].sup.+ LC Method 6-1 [00310] embedded image 1.02 259 Method 2 6-2 [00311] 1.03 223 Method 2 6-3 [00312] 1.05 267 Method 2 6-4 [00313] 0.94 243 Method 2 6-5 [00314] 0.95 207 Method 2 6-6 [00315] 0.95 207 Method 2 6-7 [00316] 0.99 223 Method 2 6-8 [00317] 1.04 267 Method 2 6-9 [00318] 0.65 225 Method 2 6-10 [00319] 0.70 239 Method 2 6-11 [00320] embedded image 0.91 203 Method 1 6-12 [00321] 0.59 203 Method 2 6-13 [00322] 0.87 223 Method 1 6-14 [00323] 1.16 362 Method 1 6-15 [00324] 0.91 203 Method 1 6-16 [00325] 0.98 253 Method 1 6-17 [00326] 1.17 326 Method 1 6-18 [00327] 1.10 237 Method 2 6-19 [00328] 0.86 225 Method 2

TABLE-US-00023 Intermediate Reaction comment 6-4 K.sub.2CO.sub.3 is used as base instead of DIPEA. The reaction is conducted for 2 h at rt. 6-5 K.sub.2CO.sub.3 is used as base instead of DIPEA. The reaction is conducted for 2 h at rt. 6-6 K.sub.2CO.sub.3 is used as base instead of DIPEA. The reaction is conducted for 2 h at rt. 6-7 The reaction is conducted for 6 h at 90° C. 6-8 The reaction is conducted for 12 h at rt. 6-9 K.sub.2CO.sub.3 is used as base instead of DIPEA. The reaction is conducted for 12 h at 80° C. 6-10 KHCO.sub.3 is used as base instead of DIPEA. The reaction is conducted in DMSO for 12 h at 80° C. 6-11 KHCO.sub.3 is used as base instead of DIPEA. The reaction is conducted in DMSO for 12 h at 80° C. 6-12 KHCO.sub.3 is used as base instead of DIPEA. The reaction is conducted in DMSO for 48 h at 45° C. 6-13 KHCO.sub.3 is used as base instead of DIPEA. The reaction is conducted in DMSO for 4 h at 45° C. 6-14 The reaction is conducted in DMSO for 12 h at 60° C. 6-15 KHCO.sub.3 is used as base instead of DIPEA. The reaction is conducted in DMSO for 12 h at 60° C. 6-16 The reaction is conducted for 24 h at 70° C. 6-17 The reaction is conducted for 18 h at 50° C. 6-18 The reaction is conducted for 2 h at 50° C.

TABLE-US-00024 Name of Name of Intermediate Name Starting Material 1 Starting Material 2 6-1 2-Chloro-6-{6,6-difluoro-3-azabicyclo- 2,6-Dichloropyridine-3- 6,6-Difluoro-3-azabicyclo- [3.1.0]hexan-3-yl}-pyridine-3-carbaldehyde carbaldehyde [3.1.0]hexane hydrochloride 6-2 6-{5-Azaspiro[2.3]hexan-5-yl}-2- 2,6-Dichloropyridine-3- 5-Azaspiro[2.3]hexane chloropyridine-3-carbaldehyde carbaldehyde trifluoroacetate 6-3 6-{5-Azaspiro[2.3]hexan-5-yl}-2- 2,6-Dibromopyridine-3- 5-Azaspiro[2.3]hexane bromopyridine-3-carbaldehyde carbaldehyde trifluoroacetate 6-4 6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan- 2,6-Difluoropyridine-3- 6,6-Difluoro-3-azabicyclo- 3-yl}-2-fluoropyridine-3-carbaldehyde carbaldehyde [3.1.0]hexane hydrochloride 6-5 6-{5-Azaspiro[2.3]hexan-5-yl}-2- 2,6-Difluoropyridine-3- 5-Azaspiro[2.3]hexane fluoropyridine-3-carbaldehyde carbaldehyde hemioxalate 6-6 6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- 2,6-Difluoropyridine-3- 3-Azabicyclo[3.1.0]hexane fluoropyridine-3-carbaldehyde carbaldehyde hydrochloride 6-7 6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- 2,6-Dichloropyridine-3- 3-Azabicyclo[3.1.0]hexane chloropyridine-3-carbaldehyde carbaldehyde hydrochloride 6-8 6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- 2,6-Dibromopyridine-3- 3-Azabicyclo[3.1.0]hexane bromopyridine-3-carbaldehyde carbaldehyde hydrochloride 6-9 6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan- 6-Chloropyridine-3- 6,6-Difluoro-3-azabicyclo- 3-yl}pyridine-3-carbaldehyde carbaldehyde [3.1.0]hexane hydrochloride 6-10 6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan- 6-Chloro-2-methyl- 6,6-Difluoro-3-azabicyclo- 3-yl}-2-methylpyridine-3-carbaldehyde pyridine-3-carbaldehyde [3.1.0]hexane hydrochloride 6-11 6-{5-Azaspiro[2.3]hexan-5-yl}-2- 6-Chloro-2-methyl- 5-Azaspiro[2.3]hexane methylpyridine-3-carbaldehyde pyridine-3-carbaldehyde trifluoroacetate 6-12 6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- 6-Chloro-2-methyl- 3-Azabicyclo[3.1.0]hexane methylpyridine-3-carbaldehyde pyridine-3-carbaldehyde hydrochloride 6-13 6-{3-Azabicyclo[3.1.0]hexan-3-yl}-4- 4,6-Dichloropyridine-3- 3-Azabicyclo[3.1.0]hexane chloropyridine-3-carbaldehyde carbaldehyde hydrochloride 6-14 2-{6,6-Difluoro-3-azabicyclo-[3.1.0]hexan- 2-Chloro-5-iodo-4- 6,6-Difluoro-3-azabicyclo- 3-yl}-5-iodo-4-methylpyridine-3- methylpyridine-3- [3.1.0]hexane hydrochloride carbonitrile carbonitrile 6-15 6-{3-Azabicyclo[3.1.0]hexan-3-yl}-4- 6-Chloro-4-methyl- 3-Azabicyclo[3.1.0]hexane methylpyridine-3-carbaldehyde pyridine-3-carbaldehyde hydrochloride 6-16 6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan- 6-Chloro-2,4-dimethyl- 6,6-Difluoro-3-azabicyclo- 3-yl}-2,4-dimethylpyridine-3-carbaldehyde pyridine-3-carbaldehyde [3.1.0]hexane hydrochloride 6-17 2-{3-Azabicyclo[3.1.0]hexan-3-yl}-5-iodo- 2-Chloro-5-iodo-4- 3-Azabicyclo[3.1.0]hexane 4-methylpyridine-3-carbonitrile methylpyridine-3- hydrochloride carbonitrile 6-18 2-Chloro-6-[(1R,5S,6R)-6-methyl-3- 2,6-Dichloropyridine-3- (1R,5S,6R)-6-Methyl-3- azabicyclo[3.1.0]hexan-3-yl]pyridine-3- carbaldehyde azabicyclo[3.1.0]hexane carbaldehyde hydrochloride (Obtained by separation of the diastereomers of tert- butyl 6-methyl-3- azabicyclo[3.1.0]hexane-3- carboxylate by standard RP chromatography and cleavage of the protecting group with HCl in EtOAc) 6-19 6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- 6-Fluoropyridine-3- 6,6-Difluoro-3-azabicyclo- yl}pyridine-3-carbaldehyde carbaldehyde [3.1.0]hexane hydrochloride

Intermediate 7

(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-ethenylpyridin-3-yl)methanol

[0323] ##STR00329##

[0324] In a microwave vial a mixture of (2-bromo-6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}pyridin-3-yl)methanol (100 mg), potassium vinyltrifluoroborate (60 mg), K.sub.2CO.sub.3 (125 mg) and THF (5 mL) is purged for 10 minutes with argon. 1,1′-Bis(diphenylphosphino)ferrocenepalladium(II) dichloride (Pd(dppf)Cl.sub.2, 15 mg) is added, the vial is sealed and the mixture is stirred at 60° C. for 12 h. After cooling to rt the mixture is diluted with MeOH and purified by HPLC on reversed phase (ACN, water) to give the title compound. LC (Method 2): t.sub.R=0.61 min; Mass spectrum (ESI+): m/z=253 [M+H].sup.+.

[0325] Intermediates 7-1 to 7-9 are prepared in analogy to Intermediate 7:

TABLE-US-00025 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M + H].sup.+ LC Method 7-1 [00330] embedded image 0.74 340 Method 2 7-2 [00331] 0.76 339 Method 2 7-3 [00332] 1.02 215 Method 1 7-4 [00333] 0.77 339 Method 2 7-5 [00334] 1.07 340 Method 2 7-6 [00335] 0.27 217 Method 2 7-7 [00336] 0.92 375 Method 2 7-8 [00337] 0.81 375 Method 2 7-9 [00338] 0.96 and 0.98 469 Method 2 [00339] (mixture of isomers)

TABLE-US-00026 Intermediate Reaction comment 7-1 The reaction is conducted at 80° C. 7-2 The reaction is conducted at 80° C. 7-4 The reaction is conducted at 90° C. 7-5 The reaction is conducted at 100° C. for 14 h. 7-6 The reaction is conducted at 80° C. 7-7 Na.sub.2CO.sub.3 is used instead of K.sub.2CO.sub.3, vinylboronic acid pinacolester instead of potassium vinyltrifluoroborate and 1,4-dioxane instead of THF. The reaction is conducted at 100° C. for 12 h. 7-8 The reaction is conducted at 100° C. for 4 h. 7-9 The reaction is conducted at 80° C. for 3 days. The product is obtained as a mixture of isomers and used as such in the next step.

TABLE-US-00027 Intermediate Name Name of Starting Material 7-1 Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- ethenylpyridin-3-yl)methyl]-1H-1,2,3-triazole-4- chloropyridin-3-yl)methyl]-1H-1,2,3-triazole-4- carboxylate carboxylate 7-2 Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- ethenylpyridin-3-yl)methyl]-1H-pyrazole-4- chloropyridin-3-yl)methyl]-1H-pyrazole-4- carboxylate carboxylate 7-3 6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- 6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- ethenylpyridine-3-carbaldehyde chloropyridine-3-carbaldehyde 7-4 Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- ethenylpyridin-3-yl)methyl]-1H-pyrazole-4- chloropyridin-3-yl)methyl]-1H-pyrazole-4- carboxylate carboxylate 7-5 Ethyl 1-[(5-{3-azabicyclo[3.1.0]hexan-3-yl}-3- Ethyl 1-[(5-{3-azabicyclo[3.1.0]hexan-3-yl}-3- ethenylpyrazin-2-yl)methyl]-1H-pyrazole-4- chloropyrazin-2-yl)methyl]-1H-pyrazole-4- carboxylate carboxylate 7-6 (6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- (6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- ethenylpyridin-3-yl)methanol bromopyridin-3-yl)methanol 7-7 Ethyl 1-[(6-{6,6-difluoro-3- Ethyl 1-[(2-chloro-6-{6,6-difluoro-3- azabicyclo[3.1.0]hexan-3-yl}-2-ethenylpyridin- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- 3-yl)methyl]-1H-pyrazole-4-carboxylate yl)methyl]-1H-pyrazole-4-carboxylate 7-8 Ethyl 1-[(6-{6,6-difluoro-3- Ethyl 1-[(2-chloro-6-{6,6-difluoro-3- azabicyclo[3.1.0]hexan-3-yl}-2-ethenylpyridin- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- 3-yl)methyl]-1H-imidazole-4-carboxylate yl)methyl]-1H-imidazole-4-carboxylate 7-9 Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- ethenylpyridin-3-yl)methyl]-1-{[2- chloropyridin-3-yl)methyl]-1-{[2- (trimethylsilyl)ethoxy]methyl}-1H-pyrazole-5- (trimethylsilyl)ethoxy]methyl}-1H-pyrazole-5- carboxylate and carboxylate and Ethyl 5-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 5-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- ethenylpyridin-3-yl)methyl]-1-{[2- chloropyridin-3-yl)methyl]-1-{[2- (trimethylsilyl)ethoxy]methyl}-1H-pyrazole-3- (trimethylsilyl)ethoxy]methyl}-1H-pyrazole-3- carboxylate (mixture of isomers) carboxylate (mixture of isomers)

Intermediate 8

Ethyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-ethenylpyridin-3-yl)methyl]-1H-pyrazole-4-carboxylate

[0326] ##STR00340##

[0327] Under argon atmosphere (6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-ethenylpyridin-3-yl)methanol (877 mg) and DIPEA (1.6 mL) are dissolved in DCM (25 mL). The mixture is cooled to 0° C. and treated dropwise with methanesulfonylchloride (CH.sub.3—SO.sub.2Cl, 318 μL). After stirring for 15 minutes ethyl 1H-pyrazole-4-carboxylate (555 mg) is added and the mixture is stirred for 3 h at rt. Then the mixture is partitioned between water and DCM. The organic phase is dried (MgSO.sub.4), concentrated and the residue is chromatographed on silica gel (petroleum ether/EtOAc 100:0.fwdarw.70:30) to give the title compound. LC (Method 2): t.sub.R=0.93 min; Mass spectrum (ESI+): m/z=375 [M+H].sup.+.

Intermediates 9

Ethyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-formylpyridin-3-yl)methyl]-1H-pyrazole-4-carboxylate

[0328] ##STR00341##

[0329] OsO.sub.4 (4% in water, 283 μL) is added to a mixture of ethyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-ethenylpyridin-3-yl)methyl]-1H-pyrazole-4-carboxylate (700 mg) in 1,4-dioxane (3.4 mL) and water (3.4 mL). The mixture is stirred for 30 minutes, treated with NaIO.sub.4 (1.2 g) and stirred for 3 h at rt. The mixture is partitioned between water and EtOAc/MeOH (9:1). After separation of the phases, the organic phase is dried (MgSO.sub.4) and concentrated to give the title compound. LC (Method 2): t.sub.R=1.06 min; Mass spectrum (ESI+): m/z=377 [M+H].sup.+.

[0330] Intermediates 9-1 to 9-18 are prepared in analogy to Intermediate 9:

TABLE-US-00028 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M + H].sup.+ LC Method 9-1 [00342] embedded image 0.90 342 Method 2 9-2 [00343] 0.81 Method 1 9-3 [00344] 0.87 342 Method 1 9-4 [00345] 1.08 341 Method 1 9-5 [00346] 0.95 377 Method 1 9-6 [00347] 0.95 341 Method 1 9-7 [00348] 342 9-8 [00349] 0.87 341 Method 2 9-9 [00350] 1.00 378 Method 2 9-10 [00351] 0.99 340 [M − H].sup.− Method 1 9-11 [00352] embedded image 0.98 341 Method 1 9-12 [00353] 0.98 342 Method 1 9-13 [00354] 0.88 342 Method 2 9-14 [00355] 1.05 377 Method 2 9-15 [00356] 0.87 377 Method 2 9-16 [00357] 1.12 342 Method 1 9-17 [00358] 1.08 378 Method 1 9-18 [00359] 1.08 and 1.15 471 Method 2 [00360] (mixture of isomers)

TABLE-US-00029 Intermediate Reaction comment 9-3 The mixture is stirred for 12 h after addition of NaIO.sub.4. 9-9 The mixture is stirred for 12 h after addition of NaIO.sub.4. 9-10 The mixture is stirred for 12 h after addition of NaIO.sub.4. 9-13 The mixture is stirred for 12 h after addition of NaIO.sub.4. 9-16 The mixture is stirred for 16 h after addition of NaIO.sub.4. 9-18 The mixture is stirred for 12 h after addition of NaIO.sub.4. The product is obtained as a mixture of isomers. Intermediate Name Name of Starting Material 9-1 Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- formylpyridin-3-yl)methyl]-1H-1,2,3-triazole-4- ethenylpyridin-3-yl)methyl]-1H-1,2,3-triazole- carboxylate 4-carboxylate 9-2 1-[(2-{3-Azabicyclo[3.1.0]hexan-3-yl}-4- 1-[(2-{3-Azabicyclo[3.1.0]hexan-3-yl}-4-[(1E)- formylpyrimidin-5-yl)methyl]-N-[(4R)-1-methyl- 2-phenylethenyl]pyrimidin-5-yl)methyl]-N- 1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]-1H- [(4R)-1-methyl-1H,4H,5H,6H-cyclopenta- imidazole-4-carboxamide [d]imidazol-4-yl]-1H-imidazole-4-carboxamide 9-3 Ethyl 1-[(2-{3-azabicyclo[3.1.0]hexan-3-yl}-4- Ethyl 1-[(2-{3-azabicyclo[3.1.0]hexan-3-yl}-4- formylpyrimidin-5-yl)methyl]-1H-imidazole-4- [(1E)-2-phenylethenyl]pyrimidin-5-yl)methyl]- carboxylate 1H-imidazole-4-carboxylate 9-4 Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- formylpyridin-3-yl)methyl]-1H-pyrazole-4- ethenylpyridin-3-yl)methyl]-1H-pyrazole-4- carboxylate carboxylate 9-5 Ethyl 1-[(6-{6,6-difluoro-3- Ethyl 1-[(6-{6,6-difluoro-3- azabicyclo[3.1.0]hexan-3-yl}-2-formylpyridin-3- azabicyclo[3.1.0]hexan-3-yl}-2-ethenylpyridin- yl)methyl]-1H-imidazole-4-carboxylate 3-yl)methyl]-1H-imidazole-4-carboxylate 9-6 Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- formylpyridin-3-yl)methyl]-1H-imidazole-4- ethenylpyridin-3-yl)methyl]-1H-imidazole-4- carboxylate carboxylate 9-7 Ethyl 1-[(2-{3-azabicyclo[3.1.0]hexan-3-yl}-4- Ethyl 1-[(2-{3-azabicyclo[3.1.0]hexan-3-yl}-4- formylpyrimidin-5-yl)methyl]-1H-pyrazole-4- [(1E)-2-phenylethenyl]pyrimidin-5-yl)methyl]- carboxylate 1H-pyrazole-4-carboxylate 9-8 Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- formylpyridin-3-yl)methyl]-1H-pyrazole-4- ethenylpyridin-3-yl)methyl]-1H-pyrazole-4- carboxylate carboxylate 9-9 Ethyl 1-[(6-{6,6-difluoro-3- Ethyl 1-[(6-{6,6-difluoro-3- azabicyclo[3.1.0]hexan-3-yl}-2-formylpyridin-3- azabicyclo[3.1.0]hexan-3-yl}-2-ethenylpyridin- yl)methyl]-1H-1,2,3-triazole-4-carboxylate 3-yl)methyl]-1H-1,2,3-triazole-4-carboxylate 9-10 Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- formylpyridin-3-yl)methyl]-1H-1,2,3-triazole-4- ethenylpyridin-3-yl)methyl]-1H-1,2,3-triazole- carboxylate 4-carboxylate 9-11 Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- formylpyridin-3-yl)methyl]-1H-imidazole-4- ethenylpyridin-3-yl)methyl]-1H-imidazole-4- carboxylate carboxylate 9-12 Ethyl 1-[(5-{3-azabicyclo[3.1.0]hexan-3-yl}-3- Ethyl 1-[(5-{3-azabicyclo[3.1.0]hexan-3-yl}-3- formylpyrazin-2-yl)methyl]-1H-pyrazole-4- ethenylpyrazin-2-yl)methyl]-1H-pyrazole-4- carboxylate carboxylate 9-13 Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- formylpyridin-3-yl)methyl]-1H-1,2,3-triazole-4- ethenylpyridin-3-yl)methyl]-1H-1,2,3-triazole- carboxylate 4-carboxylate 9-14 Ethyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- Ethyl 1-[(6-{6,6-difluoro-3- hexan-3-yl}-2-formylpyridin-3-yl)methyl]-1H- azabicyclo[3.1.0]hexan-3-yl}-2-ethenylpyridin- pyrazole-4-carboxylate 3-yl)methyl]-1H-pyrazole-4-carboxylate 9-15 Ethyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- Ethyl 1-[(6-{6,6-difluoro-3- hexan-3-yl}-2-formylpyridin-3-yl)methyl]-1H- azabicyclo[3.1.0]hexan-3-yl}-2-ethenylpyridin- imidazole-4-carboxylate 3-yl)methyl]-1H-imidazole-4-carboxylate 9-16 Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- formylpyridin-3-yl)methyl]-1,2-oxazole-5- [(1E)-2-phenylethenyl]pyridin-3-yl)methyl]-1,2- carboxylate oxazole-5-carboxylate 9-17 Ethyl 3-[(6-{6,6-difluoro-3- Ethyl 3-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- azabicyclo[3.1.0]hexan-3-yl}-2-formylpyridin-3- hexan-3-yl}-2-[(1E)-2-phenylethenyl]pyridin-3- yl)methyl]-1,2-oxazole-5-carboxylate yl)methyl]-1,2-oxazole-5-carboxylate 9-18 Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- formylpyridin-3-yl)methyl]-1-{[2-(trimethyl- ethenylpyridin-3-yl)methyl]-1-{[2-(trimethyl- silyl)ethoxy]methyl}-1H-pyrazole-5-carboxylate and silyl)ethoxy]methyl}-1H-pyrazole-5-carboxylate and Ethyl 5-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 5-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- formylpyridin-3-yl)methyl]-1-{[2-(trimethylsilyl)- ethenylpyridin-3-yl)methyl]-1-{[2-(trimethyl- ethoxy]methyl}-1H-pyrazole-3-carboxylate silyl)ethoxy]methyl}-1H-pyrazole-3-carboxylate (mixture of isomers) (mixture of isomers)

Intermediate 10

Ethyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-(hydroxymethyl)pyridin-3-yl)methyl]-1H-pyrazole-4-carboxylate

[0331] ##STR00361##

[0332] NaBH.sub.4 (40 mg) is added portionwise to a ice-cooled mixture of ethyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-formylpyridin-3-yl)methyl]-1H-pyrazole-4-carboxylate (200 mg) in EtOH (5 mL). The mixture is stirred for 2 h at rt, cooled to 0° C., treated with 4 M aqueous HCl (599 μL) and stirred for 5 minutes. 4 M aqueous NaOH (599 μL) is added and the mixture is diluted with EtOAc. After drying (MgSO.sub.4) the mixture is filtered and concentrated. The residue is partitioned between EtOAc and saturated aqueous NaHCO.sub.3. The organic phase is dried (MgSO.sub.4) and concentrated to give the title compound. LC (Method 2): t.sub.R=0.75 min; Mass spectrum (ESI+): m/z=379 [M+H].sup.+.

[0333] Intermediates 10-1 to 10-11 are prepared in analogy to Intermediate 10:

TABLE-US-00030 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M + H].sup.+ LC Method 10-1 [00362] embedded image 0.99 343 Method 1 10-2 [00363] 0.71 380 Method 2 10-3 [00364] 0.68 344 Method 2 10-4 [00365] 0.82 344 Method 2 10-5 [00366] 0.91 344 Method 1 10-6 [00367] 0.66 344 Method 2 10-7 [00368] 0.75 379 Method 2 10-8 [00369] 0.67 379 Method 2 10-9 [00370] 1.01 380 Method 1 10-10 [00371] 1.05 344 Method 1 10-11 [00372] embedded image 0.89 and 0.93 473 Method 2 [00373] (mixture of isomers)

TABLE-US-00031 Intermediate Reaction comment 10-3 The reaction is conducted in THF/MeOH 2:1. 10-3 The reaction is conducted in THF for 12 h at rt. 10-5 The reaction is conducted in THF/EtOH for 30 minutes at rt. 10-7 The reaction is conducted in THF/MeOH for 1 h at rt. 10-8 The reaction is conducted in THF/water 10:1 for 12 h at rt. 10-9 The reaction is conducted for 15 minutes at rt. The reaction is quenched with saturated aqueous NaHCO.sub.3 instead of HCl. 10-10 The reaction is conducted for 45 minutes at rt. The reaction is quenched with saturated aqueous NaHCO.sub.3 instead of HCl. 10-11 The product is obtained as a mixture of isomers.

TABLE-US-00032 Intermediate Name Name of Starting Material 10-1 Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- (hydroxymethyl)pyridin-3-yl)methyl]-1H- formylpyridin-3-yl)methyl]-1H-pyrazole-4- pyrazole-4-carboxylate carboxylate 10-2 Ethyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- Ethyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- hexan-3-yl}-2-(hydroxymethyl)pyridin-3- hexan-3-yl}-2-formylpyridin-3-yl)methyl]-1H- yl)methyl]-1H-1,2,3-triazole-4-carboxylate 1,2,3-triazole-4-carboxylate 10-3 Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- (hydroxymethyl)pyridin-3-yl)methyl]-1H-1,2,3- formylpyridin-3-yl)methyl]-1H-1,2,3-triazole-4- triazole-4-carboxylate carboxylate 10-4 Ethyl 1-[(2-{3-azabicyclo[3.1.0]hexan-3-yl}-4- Ethyl 1-[(2-{3-azabicyclo[3.1.0]hexan-3-y l}-4- (hydroxymethyl)pyrimidin-5-yl)methyl]-1H- formylpyrimidin-5-yl)methyl]-1H-pyrazole-4- pyrazole-4-carboxylate carboxylate 10-5 Ethyl 1-[(5-{3-azabicyclo[3.1.0]hexan-3-yl}-3- Ethyl 1-[(5-{3-azabicyclo[3.1.0]hexan-3-yl}-3- (hydroxymethyl)pyrazin-2-yl)methyl]-1H- formylpyrazin-2-yl)methyl]-1H-pyrazole-4- pyrazole-4-carboxylate carboxylate 10-6 Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- (hydroxymethyl)pyridin-3-yl)methyl]-1H-1,2,3- formylpyridin-3-yl)methyl]-1H-1,2,3-triazole-4- triazole-4-carboxylate carboxylate 10-7 Ethyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- Ethyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- hexan-3-yl}-2-(hydroxymethyl)pyridin-3- hexan-3-yl}-2-formylpyridin-3-yl)methyl]-1H- yl)methyl]-1H-pyrazole-4-carboxylate pyrazole-4-carboxylate 10-8 Ethyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- Ethyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- hexan-3-yl}-2-(hydroxymethyl)pyridin-3- hexan-3-yl}-2-formylpyridin-3-yl)methyl]-1H- yl)methyl]-1H-imidazole-4-carboxylate imidazole-4-carboxylate 10-9 Ethyl 3-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- Ethyl 3-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- hexan-3-yl}-2-(hydroxymethyl)pyridin-3- hexan-3-yl}-2-formylpyridin-3-yl)methyl]-1,2- yl)methyl]-1,2-oxazole-5-carboxylate oxazole-5-carboxylate 10-10 Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- (hydroxymethyl)pyridin-3-yl)methyl]-1,2- formylpyridin-3-yl)methyl]-1,2-oxazole-5- oxazole-5-carboxylate carboxylate 10-11 Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- (hydroxymethyl)pyridin-3-yl)methyl]-1-{[2- formylpyridin-3-yl)methyl]-1-{[2-(trimethylsilyl)- (trimethylsilyl)ethoxy]methyl}-1H-pyrazole-5- ethoxy]methyl}-1H-pyrazole-5-carboxylate carboxylate and and Ethyl 5-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 5-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- formylpyridin-3-yl)methyl]-1-{[2-(trimethylsilyl)- (hydroxymethyl)pyridin-3-yl)methyl]-1-{[2- ethoxy]methyl}-1H-pyrazole-3-carboxylate (trimethylsilyl)ethoxy]methyl}-1H-pyrazole-3- (mixture of isomers) carboxylate (mixture of isomers)

Intermediate 11

Ethyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-(methoxymethyl)pyridin-3-yl)methyl]-1H-pyrazole-4-carboxylate

[0334] ##STR00374##

[0335] To a solution of ethyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-(hydroxymethyl)pyridin-3-yl)methyl]-1H-pyrazole-4-carboxylate (89 mg) in DMF (250 μL) is added at 0° C. NaH (60% in mineral oil, 23 mg). The mixture is stirred for 15 minutes, treated with CH.sub.3I (17 μL) and stirred for 12 h at rt. The solvents are evaporated in vacuo to give the crude product, which is directly used in the next step.

[0336] LC (Method 2): t.sub.R=0.83 min; Mass spectrum (ESI+): m/z=393 [M+H].sup.+.

[0337] Intermediate 11-1 is prepared in analogy to Intermediate 11:

TABLE-US-00033 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M + H].sup.+ LC Method 11-1 [00375] embedded image 0.75 393 Method 2

TABLE-US-00034 Intermediate Reaction comment 11-1 The reaction is stirred for 1 h at rt after addition of CH.sub.3I.

TABLE-US-00035 Intermediate Name Name of Starting Material 11-1 Ethyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- Ethyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- hexan-3-yl}-2-(methoxymethyl)pyridin-3- hexan-3-yl}-2-(hydroxymethyl)pyridin-3- yl)methyl]-1H-imidazole-4-carboxylate yl)methyl]-1H-imidazole-4-carboxylate

Intermediate 12

Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2-(difluoromethyl)pyridin-3-yl)methyl]-1H-1,2,3-triazole-4-carboxylate

[0338] ##STR00376##

[0339] In a microwave vial ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2-formylpyridin-3-yl)methyl]-1H-1,2,3-triazole-4-carboxylate (763 mg) is dissolved in DCM (10 mL). Diethylaminosulfurtrifluoride (DAST, 1 mL) is added, the vial is sealed and the mixture is heated to 50° C. for 12 h. After cooling to rt the mixture is carefully treated with 1 N aqueous NaHCO.sub.3 until gas evolution has stopped. Then the mixture is partitioned between saturated aqueous NaHCO.sub.3 and DCM. The phases are separated and the aqueous phase is extracted with DCM. The combined organic phases are washed with brine, dried (MgSO.sub.4) and concentrated. The residue is chromatographed on silica gel (petroleum ether/EtOAc 70:30.fwdarw.30:70) to give the title compound.

[0340] LC (Method 2): t.sub.R=1.05 min; Mass spectrum (ESI+): m/z=364 [M+H].sup.+.

[0341] Intermediates 12-1 to 12-10 are prepared in analogy to Intermediate 12:

TABLE-US-00036 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M + H].sup.+ LC Method 12-1 [00377] embedded image 0.88 364 Method 2 12-2 [00378] 1.08 363 Method 2 12-3 [00379] 0.99 399 Method 1 12-4 [00380] 1.07 399 Method 1 12-5 [00381] 1.00 363 Method 1 12-6 [00382] 1.10 364 Method 2 12-7 [00383] 1.09 363 Method 1 12-8 [00384] 1.03 363 Method 1 12-9 [00385] 1.17 364 Method 1 12-10 [00386] 1.12 400 Method 1

TABLE-US-00037 Intermediate Reaction comment 12-9 The reaction is conducted at 0° C. and is stirred for 1 h. 12-10 The reaction is conducted at 0° C. and is stirred for 30 minutes. Intermediate Name Name of Starting Material 12-1 Ethyl 1-[(2-{3-azabicyclo[3.1.0]hexan-3-yl}-4- Ethyl 1-[(2-{3-azabicyclo[3.1.0]hexan-3-yl}-4- (difluoromethyl)pyrimidin-5-yl)methyl]-1H- formylpyrimidin-5-yl)methyl]-1H-imidazole-4- imidazole-4-carboxylate carboxylate 12-2 Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- (difluoromethyl)pyridin-3-yl)methyl]-1H- formylpyridin-3-yl)methyl]-1H-pyrazole-4- pyrazole-4-carboxylate carboxylate 12-3 Ethyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- Ethyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- hexan-3-yl}-2-(difluoromethyl)pyridin-3- hexan-3-yl}-2-formylpyridin-3-yl)methyl]-1H- yl)methyl]-1H-imidazole-4-carboxylate imidazole-4-carboxylate 12-4 Ethyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- Ethyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- hexan-3-yl}-2-(difluoromethyl)pyridin-3- hexan-3-yl}-2-formylpyridin-3-yl)methyl]-1H- yl)methyl]-1H-pyrazole-4-carboxylate pyrazole-4-carboxylate 12-5 Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- (difluoromethyl)pyridin-3-yl)methyl]-1H- formylpyridin-3-yl)methyl]-1H-imidazole-4- imidazole-4-carboxylate carboxylate 12-6 Ethyl 1-[(2-{3-azabicyclo[3.1.0]hexan-3-yl}-4- Ethyl 1-[(2-{3-azabicyclo[3.1.0]hexan-3-yl}-4- (difluoromethyl)pyrimidin-5-yl)methyl]-1H- formylpyrimidin-5-yl)methyl]-1H-pyrazole-4- pyrazole-4-carboxylate carboxylate 12-7 Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- (difluoromethyl)pyridin-3-yl)methyl]-1H- formylpyridin-3-yl)methyl]-1H-pyrazole-4- pyrazole-4-carboxylate carboxylate 12-8 Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- (difluoromethyl)pyridin-3-yl)methyl]-1H- formylpyridin-3-yl)methyl]-1H-imidazole-4- imidazole-4-carboxylate carboxylate 12-9 Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- (difluoromethyl)pyridin-3-yl)methyl]-1,2- formylpyridin-3-yl)methyl]-1,2-oxazole-5- oxazole-5-carboxylate carboxylate 12-10 Ethyl 3-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- Ethyl 3-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- hexan-3-yl}-2-(difluoromethyl)pyridin-3-yl)- hexan-3-yl}-2-formylpyridin-3-yl)methyl]-1,2- methyl]-1,2-oxazole-5-carboxylate oxazole-5-carboxylate

Intermediate 13

Ethyl 2-chloro-4-[(1E)-2-phenylethenyl]pyrimidine-5-carboxylate

[0342] ##STR00387##

[0343] In a microwave vial a mixture of ethyl 2,4-dichloropyrimidine-5-carboxylate (2.5 g), potassium trans-beta-styryltrifluoroborate (2.5 g), Na.sub.2CO.sub.3 (2 M aqueous solution, 12.5 mL) and 1,4-dioxane (50 mL) is purged for 10 minutes with argon. Bis(di-tert-butyl(4-dimethylaminophenyl)phosphine)dichloropalladium(II) (Pd(amphos).sub.2Cl.sub.2, 300 mg) is added, the vial is sealed and the mixture is heated to 50° C. for 2 h. After cooling to rt the mixture is partitioned between EtOAc and water. The organic phase is washed with brine, dried (MgSO.sub.4) and concentrated. The residue is chromatographed on silica gel (petroleum ether/EtOAc 80:20.fwdarw.60:40) to give the title compound.

[0344] LC (Method 2): t.sub.R=1.21 min; Mass spectrum (ESI+): m/z=289 [M+H].sup.+.

[0345] Intermediates 13-1 to 13-2 are prepared in analogy to Intermediate 13:

TABLE-US-00038 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M + H].sup.+ LC Method 13-1 [00388] embedded image 1.27 416 Method 1 13-2 [00389] 1.20 452 Method 1

TABLE-US-00039 Intermediate Reaction comment 13-1 The reaction is conducted for 15 h at 75° C. 13-2 The reaction is conducted for 18 h at 80° C. Intermediate Name Name of Starting Material 13-1 Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- [(1E)-2-phenylethenyl]pyridin-3-yl)methyl]-1,2- chloropyridin-3-yl)methyl]-1,2-oxazole-5- oxazole-5-carboxylate carboxylate 13-2 Ethyl 3-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- Ethyl 3-[(2-chloro-6-{6,6-difluoro-3- hexan-3-yl}-2-[(1E)-2-phenylethenyl]pyridin-3- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- yl)methyl]-1,2-oxazole-5-carboxylate yl)methyl]-1,2-oxazole-5-carboxylate

Intermediate 14

Ethyl 2-{3-azabicyclo[3.1.0]hexan-3-yl}-4-[(1E)-2-phenylethenyl]pyrimidine-5-carboxylate

[0346] ##STR00390##

[0347] Under argon atmosphere a mixture of ethyl 2-chloro-4-[(1E)-2-phenylethenyl]pyrimidine-5-carboxylate (2.56 g), 3-azabicyclo[3.1.0]hexane hydrochloride (1.3 g) and KHCO.sub.3 (2.3 g) in THF (30 mL) is stirred for 12 h at rt. The mixture is partitioned between saturated aqueous NH.sub.4Cl and EtOAc and the phases are separated. The organic phase is washed with brine, dried (MgSO.sub.4), concentrated and the residue is chromatographed on silica gel (petroleum ether/EtOAc 80:20.fwdarw.60:40) to give the title compound.

[0348] LC (Method 2): t.sub.R=1.27 min; Mass spectrum (ESI+): m/z=336 [M+H].sup.+.

Intermediate 15

(2-{3-Azabicyclo[3.1.0]hexan-3-yl}-4-[(1E)-2-phenylethenyl]pyrimidin-5-yl)methanol

[0349] ##STR00391##

[0350] Under argon atmosphere a mixture of ethyl 2-{3-azabicyclo[3.1.0]hexan-3-yl}-4-[(1E)-2-phenylethenyl]pyrimidine-5-carboxylate (1.96 g) in THF (40 mL) is treated dropwise with diisobutylaluminiumhydride (DIBAH, 1 M in THF, 25 mL). The mixture is stirred for 2 h at rt, cooled to 0° C. and treated dropwise with 4 M aqueous HCl (15 mL). Then the mixture is stirred for 5 minutes and 4 M aqueous NaOH (15 mL) is added. The mixture is partitioned between brine and DCM and the phases are separated. The organic phase is dried (MgSO.sub.4), concentrated and the residue is chromatographed on silica gel (DCM/MeOH 98:2.fwdarw.90:10) to give the title compound.

[0351] LC (Method 2): t.sub.R=0.84 min; Mass spectrum (ESI+): m/z=294 [M+H].sup.+.

[0352] Intermediates 15-1 to 15-2 are prepared in analogy to Intermediate 15:

TABLE-US-00040 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M + H].sup.+ LC Method 15-1 [00392] embedded image 0.72 234 Method 2 15-2 [00393] 0.60 206 Method 1

TABLE-US-00041 Intermediate Name Name of Starting Material 15-1 (6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- propylpyridin-3-yl)methanol propylpyridine-3-carboxylate 15-2 (2-{5-Azaspiro[2.3]hexan-5-yl}-4- Ethyl 2-{5-azaspiro[2.3]hexan-5-yl}-4- methylpyrimidin-5-yl)methanol methylpyrimidine-5-carboxylate

Intermediate 16

1-[(2-{3-Azabicyclo[3.1.0]hexan-3-yl}-4-[(1E)-2-phenylethenyl]pyrimidin-5-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide

[0353] ##STR00394##

[0354] Under argon atmosphere a mixture of 1-[(2-{3-azabicyclo[3.1.0]hexan-3-yl}-4-[(1E)-2-phenylethenyl]pyrimidin-5-yl)-methyl]-1H-imidazole-4-carboxylic acid (240 mg) and DIPEA (380 μL) in DMF (3 mL) is treated with O-(7-azabenzotriazol-1-yl)-N,N,N′,N′-tetramethyluronium-hexafluorophosphat (HATU, 280 mg). The mixture is stirred for 5 minutes and then treated with (4R)-1-methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-amine dihydrochloride (145 mg). After stirring for 1 h the mixture is partitioned between water and DCM. The organic phase is washed with brine, dried (MgSO.sub.4), concentrated and the residue is chromatographed on silica gel (DCM/MeOH 98:2.fwdarw.70:30) to give the title compound. LC (Method 1): t.sub.R=1.02 min; Mass spectrum (ESI+): m/z=507 [M+H].sup.+.

Intermediate 17

Ethyl 1-[(5-{5-azaspiro[2.3]hexan-5-yl}pyridin-2-yl)methyl]-1H-pyrazole-4-carboxylate

[0355] ##STR00395##

[0356] To a solution of (5-{5-azaspiro[2.3]hexan-5-yl}pyridin-2-yl)methanol (110 mg), ethyl 1H-pyrazole-4-carboxylate (122 mg) and triphenylphosphine (296 mg) in THF (2 mL) is added dropwise at 0° C. DIAD (222 μL). The mixture is stirred for 1 h while warming to rt. Then the mixture is diluted with MeOH and purified by HPLC on reversed phase (ACN, water) to give the title compound. LC (Method 2): t.sub.R=0.79 min; Mass spectrum (ESI+): m/z=313 [M+H].sup.+.

[0357] Intermediate 17-1 is prepared in analogy to Intermediate 17:

TABLE-US-00042 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M + H].sup.+ LC Method 17-1 [00396] embedded image 0.99 338 Method 1

TABLE-US-00043 Name of Name of Intermediate Name Starting Material 1 Starting Material 2 17-1 Ethyl 1-[(5-{3-azabicyclo[3.1.0]hexan- 3-{3-Azabicyclo[3.1.0]hexan-3- Ethyl 1H-pyrazole-4- 3-yl}-6-cyanopyridin-2-yl)methyl]-1H- yl}-6-(hydroxymethyl)pyridine- carboxylate pyrazole-4-carboxylate 2-carbonitrile

Intermediate 18

Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2-ethylpyridin-3-yl)methyl]-1H-1,2,3-triazole-4-carboxylate

[0358] ##STR00397##

[0359] In a microwave vial a mixture of ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2-chloropyridin-3-yl)methyl]-1H-1,2,3-triazole-4-carboxylate (85 mg), ethylboronic acid (55 mg), K.sub.2CO.sub.3 (170 mg) and 1,4-dioxane (3 mL) is purged for 10 minutes with argon. 1,1′-Bis(diphenylphosphino)ferrocenepalladium(II) dichloride (Pd(dppf)Cl.sub.2, 14 mg) is added, the vial is sealed and the mixture is heated to 80° C. for 12 h. Ethylboronic acid (65 mg), K.sub.2CO.sub.3 (100 mg) and 1,1′-Bis(diphenylphosphino)ferrocenepalladium(II) dichloride (Pd(dppf)Cl.sub.2, 16 mg) are added and the mixture is heated for 5 h to 90° C. After cooling to rt the mixture is partitioned between water and EtOAc. The organic phase is washed with brine, dried (MgSO.sub.4), concentrated and the residue is chromatographed on silica gel (petroleum ether/EtOAc 85:15.fwdarw.60:40) to give the title compound. LC (Method 2): t.sub.R=0.72 min; Mass spectrum (ESI+): m/z=342 [M+H].sup.+.

[0360] Intermediates 18-1 to 18-4 are prepared in analogy to Intermediate 18:

TABLE-US-00044 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M + H].sup.+ LC Method 18-1 [00398] embedded image 0.72 342 Method 2 18-2 [00399] 0.76 378 Method 2 18-3 [00400] 0.66 217 Method 2 18-4 [00401] 0.86 253 Method 2

TABLE-US-00045 Intermediate Reaction comment 18-1 The reaction is conducted for 12 h at 80° C. 18-2 The reaction is conducted for 12 h at 80° C. 18-3 The reaction is conducted for 5 h at 70° C. 18-4 The reaction is conducted for 16 h at 80° C.

TABLE-US-00046 Intermediate Name Name of Starting Material 18-1 Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- ethylpyridin-3-yl)methyl]-1H-1,2,3-triazole-4- bromopyridin-3-yl)methyl]-1H-1,2,3-triazole-4- carboxylate carboxylate 18-2 Ethyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- Ethyl 1-[(2-bromo-6-{6,6-difluoro-3- hexan-3-yl}-2-ethylpyridin-3-yl)methyl]-1H- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- 1,2,3-triazole-4-carboxylate yl)methyl]-1H-1,2,3-triazole-4-carboxylate 18-3 6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- 6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- ethylpyridine-3-carbaldehyde chloropyridine-3-carbaldehyde 18-4 6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}- 2-Bromo-6-{6,6-difluoro-3-azabicyclo[3.1.0]- 2-ethylpyridine-3-carbaldehyde hexan-3-yl}pyridine-3-carbaldehyde

Intermediate 19

Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-ethylpyridin-3-yl)methyl]-1H-pyrazole-4-carboxylate

[0361] ##STR00402##

[0362] In a microwave vial a mixture of ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-chloropyridin-3-yl)methyl]-1H-pyrazole-4-carboxylate (1.43 g), diethylzinc (1 M solution in n-hexane, 6.18 mL) and 1,4-dioxane (60 mL) is purged for minutes with argon. 1,1′-Bis(diphenylphosphino)ferrocenepalladium(II) dichloride (Pd(dppf)Cl.sub.2, 150 mg) is added, the vial is sealed and the mixture is heated to 70° C. for 1 h. After cooling to rt the mixture is carefully treated with saturated aqueous NH.sub.4Cl. The mixture is partitioned between saturated aqueous NH.sub.4Cl and EtOAc. The aqueous phase is extracted twice with EtOAc. The combined organic phases are washed with brine, dried (MgSO.sub.4), concentrated and the residue is chromatographed on silica gel (petroleum ether/EtOAc 95:5.fwdarw.0:100) to give the title compound. LC (Method 2): t.sub.R=0.75 min; Mass spectrum (ESI+): m/z=341 [M+H].sup.+.

[0363] Intermediates 19-1 to 19-15 are prepared in analogy to Intermediate 19:

TABLE-US-00047 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M + H].sup.+ LC Method 19-1 [00403] embedded image 377 19-2 [00404] 0.67 341 Method 2 19-3 [00405] 0.59 219 Method 2 19-4 [00406] 1.06 Method 1 19-5 [00407] 1.18 403 Method 1 19-6 [00408] 1.14 391 Method 1 19-7 [00409] 0.66 341 Method 2 19-8 [00410] 0.68 Method 2 19-9 [00411] 0.70 Method 2 19-10 [00412] 0.66 Method 2 19-11 [00413] 0.85 353 Method 2 19-12 [00414] embedded image 1.10 355 Method 1 19-13 [00415] 0.80 342 Method 2 19-14 [00416] 0.80 363 Method 2 19-15 [00417] 0.87 393 Method 2

TABLE-US-00048 Intermediate Reaction comment 19-4 n-Propylzinc bromide is used instead of diethylzinc. 19-5 Cyclobutylzinc bromide is used instead of diethylzinc. 19-6 n-Propylzinc bromide is used instead of diethylzinc. 19-7 The reaction is conducted in the presence of LiCL in the same molar amount as diethylzinc. The reaction is heated for 2 h to 60° C. 19-9 2-Methylpropylzinc bromide is used instead of diethylzinc and dichloro[1,3-bis(2,6-Di-3- pentylphenyl)imidazol-2-ylidene](3-chloropyridyl)palladium(II) (Pd-PEPPSI-IPent) is used instead of 1,1′-bis(diphenylphosphino)ferrocenepalladium(II) dichloride (Pd(dppf)Cl.sub.2). The reaction is conducted in the presence of LiCL in the same molar amount as diethylzinc. The reaction is heated for 2 h to 60° C. 19-10 n-Propylzinc bromide is used instead of diethylzinc and dichloro[1,3-bis(2,6-Di-3- pentylphenyl)imidazol-2-ylidene](3-chloropyridyl)palladium(II) (Pd-PEPPSI-IPent) is used instead of 1,1′-bis(diphenylphosphino)ferrocenepalladium(II) dichloride (Pd(dppf)Cl.sub.2). The reaction is conducted in the presence of LiCL in the same molar amount as diethylzinc. The reaction is heated for 2 h to 60° C. 19-11 Cyclopropylzinc bromide is used instead of diethylzinc. 19-12 Dichloro[1,3-bis(2,6-Di-3-pentylphenyl)imidazol-2-ylidene](3-chloropyridyl)palladium(II) (Pd- PEPPSI-IPent) is used instead of 1,T-bis(diphenylphosphino)ferrocenepalladium(II) dichloride (Pd(dppf)Cl.sub.2). The reaction is conducted in the presence of LiCL in the same molar amount as diethylzinc. The reaction is heated for 1 h to 60° C. 19-13 The reaction is conducted for 2.5 h at 80° C.

TABLE-US-00049 Intermediate Name Name of Starting Material 19-1 Ethyl 1-[(6-{6,6-difluoro-3- Ethyl 1-[(2-bromo-6-{6,6-difluoro-3- azabicyclo[3.1.0]hexan-3-yl}-2-ethylpyridin-3- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- yl)methyl]-1H-pyrazole-4-carboxylate yl)methyl]-1H-pyrazole-4-carboxylate 19-2 Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- ethylpyridin-3-yl)methyl]-1H-imidazole-4- chloropyridin-3-yl)methyl]-1H-imidazole-4- carboxylate carboxylate 19-3 (6-{5-Azaspiro[2.3]hexan-5-yl}-2-ethylpyridin- (6-{5-Azaspiro[2.3]hexan-5-yl}-2-chloropyridin- 3-yl)methanol 3-yl)methanol 19-4 Ethyl 1-[(6-{6,6-difluoro-3- Ethyl 1-[(2-bromo-6-{6,6-difluoro-3- azabicyclo[3.1.0]hexan-3-yl}-2-propylpyridin-3- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- yl)methyl]-1H-imidazole-4-carboxylate yl)methyl]-1H-imidazole-4-carboxylate 19-5 Ethyl 1-[(2-cyclobutyl-6-{6,6-difluoro-3- Ethyl 1-[(2-bromo-6-{6,6-difluoro-3- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- yl)methyl]-1H-pyrazole-4-carboxylate yl)methyl]-1H-pyrazole-4-carboxylate 19-6 Ethyl 1-[(6-{6,6-difluoro-3- Ethyl 1-[(2-bromo-6-{6,6-difluoro-3- azabicyclo[3.1.0]hexan-3-yl}-2-propylpyridin-3- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- yl)methyl]-1H-pyrazole-4-carboxylate yl)methyl]-1H-pyrazole-4-carboxylate 19-7 Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- ethylpyridin-3-yl)methyl]-1H-imidazole-4- bromopyridin-3-yl)methyl]-1H-imidazole-4- carboxylate carboxylate 19-8 Ethyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- Ethyl 1-[(2-bromo-6-{6,6-difluoro-3- hexan-3-yl}-2-ethylpyridin-3-yl)methyl]-1H- azabicyclo[3.1.0]hexan-3-yl}pyridin-3-yl)- imidazole-4-carboxylate methyl]-1H-imidazole-4-carboxylate 19-9 Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- (2-methylpropyl)pyridin-3-yl)methyl]-1H- bromopyridin-3-yl)methyl]-1H-imidazole-4- imidazole-4-carboxylate carboxylate 19-10 Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- propylpyridin-3-yl)methyl]-1H-imidazole-4- bromopyridin-3-yl)methyl]-1H-imidazole-4- carboxylate carboxylate 19-11 Ethyl 1-[(6-{3-azabicyclo[3.1 0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- cyclopropylpyridin-3-yl)methyl]-1H-pyrazole-4- bromopyridin-3-yl)methyl]-1H-pyrazole-4- carboxylate carboxylate 19-12 Ethyl 1-({2-ethyl-6-[(1R,5S,6R)-6-methyl-3- Ethyl 1-({2-chloro-6-[(1R,5S,6R)-6-methyl-3- azabicyclo[3.1.0]hexan-3-yl]pyridin-3- azabicyclo[3.1.0]hexan-3-yl]pyridin-3- yl}methyl)-1H-imidazole-4-carboxylate yl}methyl)-1H-imidazole-4-carboxylate 19-13 Ethyl 3-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- Ethyl 3-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- ethylpyridin-3-yl)methyl]-1,2-oxazole-5- chloropyridin-3-yl)methyl]-1,2-oxazole-5- carboxylate carboxylate 19-14 Methyl 5-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- Methyl 5-[(2-bromo-6-{6,6-difluoro-3- hexan-3-yl}-2-ethylpyridin-3-yl)methyl]furan-2- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- carboxylate yl)methyl]furan-2-carboxylate 19-15 Ethyl 5-[(6-{6,6-difluoro-3- Ethyl 5-[(2-bromo-6-{6,6-difluoro-3- azabicyclo[3.1.0]hexan-3-yl}-2-ethylpyridin-3- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- yl)methyl]thiophene-2-carboxylate yl)methyl]thiophene-2-carboxylate

Intermediate 20

Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}pyridin-3-yl)methyl]-1H-1,2,3-triazole-4-carboxylate

[0364] ##STR00418##

[0365] A mixture of ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2-bromopyridin-3-yl)methyl]-1H-1,2,3-triazole-4-carboxylate (150 mg), 10% palladium on carbon (20 mg) in EtOH (4 mL) and THF (4 mL) is shaken under hydrogen atmosphere (3 bar) at rt for 3.5 h. The mixture is filtered, the filtrate is concentrated and the residue is purified by HPLC on reversed phase (ACN, water) to give the title compound. LC (Method 2): t.sub.R=0.68 min; Mass spectrum (ESI+): m/z=314 [M+H].sup.+.

Intermediate 21

Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-(difluoromethyl)pyridin-3-yl)methyl]-1H-1,2,3-triazole-4-carboxylate

[0366] ##STR00419##

[0367] In a microwave vial a mixture of ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-bromopyridin-3-yl)methyl]-1H-1,2,3-triazole-4-carboxylate (250 mg), CsF (290 mg) and CuF (121 mg) in NMP (4 mL) is treated with difluoromethyltrimethylsilane (435 μL). The vial is sealed and the mixture is heated to 120° C. for 1.5 h. The mixture is partitioned between half-saturated aqueous NaHCO.sub.3 and EtOAc. Then the mixture is filtered over celite and the filter cake is washed with EtOAc. The phases are separated and the aqueous phase is extracted twice with EtOAc. The combined organic phases are dried (MgSO.sub.4), concentrated and the residue is purified by HPLC on reversed phase (ACN, water) to give the title compound. LC (Method 2): t.sub.R=1.10 min; Mass spectrum (ESI+): m/z=364 [M+H].sup.+.

Intermediate 22

Ethyl 2-chloro-4-propylpyrimidine-5-carboxylate

[0368] ##STR00420##

[0369] In a microwave vial a mixture of ethyl 2,4-dichloropyrimidine-5-carboxylate (1 g), n-propylzinc bromide (0.5 M in THF, 9.5 mL) and 1,1′-bis(diphenylphosphino)ferrocenepalladium(II) dichloride (Pd(dppf)Cl.sub.2, 66 mg) in 1,4-dioxane (25 mL) is purged for 10 minutes with argon. The vial is sealed and the mixture is heated to 70° C. for 1 h. Then n-propylzinc bromide (0.5 M in THF, 5 mL) is added and the mixture is heated for 45 minutes to 70° C. After cooling to rt the mixture is partitioned between EtOAc and saturated aqueous NH.sub.4Cl. The aqueous phase is extracted twice with EtOAc. The combined organic phases are washed with brine, dried (MgSO.sub.4) and concentrated. The residue is chromatographed on silica gel (petroleum ether/EtOAc 98:2.fwdarw.90:10) to give the title compound.

[0370] LC (Method 2): t.sub.R=1.10 min; Mass spectrum (ESI+): m/z=229 [M+H].sup.+.

Intermediate 23

Ethyl 2-{3-azabicyclo[3.1.0]hexan-3-yl}-4-propylpyrimidine-5-carboxylate

[0371] ##STR00421##

[0372] Under argon atmosphere a mixture of ethyl 2-chloro-4-propylpyrimidine-5-carboxylate (546 mg), 3-azabicyclo[3.1.0]hexane hydrochloride (328 mg) and K.sub.2CO.sub.3 (663 mg) in DMF (15 mL) is stirred for 2 h at rt. The mixture is partitioned between water and EtOAc and the phases are separated. The aqueous phase is extracted twice with EtOAc. The combined organic phases are washed with brine, dried (MgSO.sub.4), concentrated and the residue is purified by HPLC on reversed phase (ACN, water) to give the title compound.

[0373] LC (Method 2): t.sub.R=1.09 min; Mass spectrum (ESI+): m/z=276 [M+H].sup.+.

Intermediate 24

Methyl 5-{3-azabicyclo[3.1.0]hexan-3-yl}-6-cyanopyridine-2-carboxylate

[0374] ##STR00422##

[0375] Under argon atmosphere a mixture of methyl 5-bromo-6-cyanopyridine-2-carboxylate (500 mg), 3-azabicyclo[3.1.0]hexane hydrochloride (289 mg) and K.sub.2CO.sub.3 (717 mg) in NMP (5 mL) is stirred for 12 h at 80° C. The mixture is poured into water. The precipitate is collected by filtration, washed with water and dried in vacuo to give the title compound. LC (Method 2): t.sub.R=0.92 min; Mass spectrum (ESI+): m/z=244 [M+H].sup.+.

Intermediate 25

3-{3-Azabicyclo[3.1.0]hexan-3-yl}-6-(hydroxymethyl)pyridine-2-carbonitrile

[0376] ##STR00423##

[0377] NaBH.sub.4 (131 mg) is added portionwise to a mixture of methyl 5-{3-azabicyclo[3.1.0]hexan-3-yl}-6-cyanopyridine-2-carboxylate (280 mg) and CaCl.sub.2 (507 mg) in THF (8 mL) and EtOH (8 mL). The mixture is stirred for 2 h at rt and for 1 h at 45° C. Then the mixture is partitioned between saturated aqueous NaHCO.sub.3 and EtOAc. The precipitate is filtered off. The aqueous phase is extracted with EtOAc. The combined organic phases are dried (MgSO.sub.4), concentrated and the residue is purified by HPLC on reversed phase (ACN, water) to give the title compound.

[0378] LC (Method 1): t.sub.R=0.81 min; Mass spectrum (ESI+): m/z=216 [M+H].sup.+.

[0379] Intermediate 25-1 is prepared in analogy to Intermediate 25:

TABLE-US-00050 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M + H].sup.+ LC Method 25-1 [00424] embedded image 0.83 395 Method 2

TABLE-US-00051 Intermediate Reaction comment 25-1 The reaction is conducted at rt for 24 h.

TABLE-US-00052 Intermediate Name Name of Starting Material 25-1 Ethyl 5-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- Ethyl 6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan- hexan-3-yl}-2-(hydroxymethyl)-pyridin-3- 3-yl}-3-{[5-(ethoxycarbonyl)thiophen-2- yl)methyl]thiophene-2-carboxylate yl]methyl}pyridine-2-carboxylate

Intermediate 26

Methyl 6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-(trifluoromethoxy)pyridine-3-carboxylate

[0380] ##STR00425##

[0381] Under argon atmosphere a mixture of methyl 6-chloro-2-(trifluoromethoxy)pyridine-3-carboxylate (1 g), 3-azabicyclo[3.1.0]hexane hydrochloride (538 mg) and K.sub.2CO.sub.3 (1.1 g) in DMF (20 mL) is stirred for 4 h at rt. The mixture is partitioned between water and EtOAc. The aqueous phase is extracted twice with EtOAc. The combined organic phases are dried (MgSO.sub.4), concentrated and the residue is purified by HPLC on reversed phase (ACN, water) to give the title compound. LC (Method 2): t.sub.R=1.18 min; Mass spectrum (ESI+): m/z=303 [M+H].sup.+.

Intermediate 27

3-[5-(Methoxymethyl)-6-(trifluoromethoxy)pyridin-2-yl]-3-azabicyclo[3.1.0]hexane

[0382] ##STR00426##

[0383] LiBH.sub.4 (250 mg) is added portionwise to a mixture of methyl 6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-(trifluoromethoxy)pyridine-3-carboxylate (384 mg) in THF (5 mL). The mixture is stirred for 12 h at rt. Then the mixture is poured into 1 N aqueous HCl and stirred vigorously for 20 minutes. Thereafter the mixture is partitioned between saturated aqueous NaHCO.sub.3 and EtOAc. The aqueous phase is extracted twice with EtOAc. The combined organic phases are dried (MgSO.sub.4), concentrated and the residue is purified by HPLC on reversed phase (ACN, water) to give the title compound. LC (Method 2): t.sub.R=1.22 min; Mass spectrum (ESI+): m/z=289 [M+H].sup.+.

Intermediate 28

Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-ethenylpyridin-3-yl)methyl]-1H-pyrazole-4-carboxylate

[0384] ##STR00427##

[0385] In a microwave vial a mixture of ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-chloropyridin-3-yl)methyl]-1H-pyrazole-4-carboxylate (5.62 g), vinylboronic acid pinacolester (2.9 mL), Na.sub.2CO.sub.3 (1 M aqueous solution, 40.5 mL) and 1,4-dioxane (75 mL) is purged for 10 minutes with argon. 1,1′-Bis(diphenylphosphino)ferrocenepalladium(II) dichloride (Pd(dppf)Cl.sub.2, 662 mg) is added, the vial is sealed and the mixture is heated to 100° C. for 12 h. After cooling to rt the mixture is partitioned between water and EtOAc. The aqueous phase is extracted twice with EtOAc. The combined organic phases are dried (MgSO.sub.4), concentrated and the residue is chromatographed on silica gel (petroleum ether/EtOAc 100:0.fwdarw.50:50) to give the title compound.

[0386] LC (Method 2): t.sub.R=0.78 min; Mass spectrum (ESI+): m/z=339 [M+H].sup.+.

[0387] Intermediates 28-1 to 28-5 are prepared in analogy to Intermediate 28:

TABLE-US-00053 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M + H].sup.+ LC Method 28-1 [00428] embedded image 0.99 Method 1 28-2 [00429] 1.01 339 Method 1 28-3 [00430] 0.79 353 Method 2 28-4 [00431] 1.04 339 Method 1 28.5 [00432] 0.82 367 Method 2

TABLE-US-00054 Intermediate Reaction comment 28-3 Isopropenylboronic acid pinacolester is used instead of vinylboronic acid pinacolester. 28-5 Isopropenylboronic acid pinacolester is used instead of vinylboronic acid pinacolester. The reaction is conducted for 18 h at 80° C.

TABLE-US-00055 Intermediate Name Name of Starting Material 28-1 Ethyl 1-[(6-{6,6-difluoro-3- Ethyl 1-[(2-bromo-6-{6,6-difluoro-3- azabicyclo[3.1.0]hexan-3-yl}-2-ethenylpyridin- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- 3-yl)methyl]-1H-imidazole-4-carboxylate yl)methyl]-1H-imidazole-4-carboxylate 28-2 Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- ethenylpyridin-3-yl)methyl]-1H-imidazole-4- chloropyridin-3-yl)methyl]-1H-imidazole-4- carboxylate carboxylate 28-3 Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- (prop-1-en-2-yl)pyridin-3-yl)methyl]-1H- chloropyridin-3-yl)methyl]-1H-pyrazole-4- pyrazole-4-carboxylate carboxylate 28-4 Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- ethenylpyridin-3-yl)methyl]-1H-imidazole-4- chloropyridin-3-yl)methyl]-1H-imidazole-4- carboxylate carboxylate 28-5 Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- methylpyridin-3-yl)methyl]-3-(prop-1-en-2-yl)- methylpyridin-3-yl)methyl]-3-bromo-1H- 1H-pyrazole-4-carboxylate pyrazole-4-carboxylate

Intermediate 29

Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-(cyanomethyl)pyridin-3-yl)methyl]-1H-pyrazole-4-carboxylate

[0388] ##STR00433##

[0389] To a solution of ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-(hydroxymethyl)pyridin-3-yl)methyl]-1H-pyrazole-4-carboxylate (400 mg), 2-hydroxy-2-methylpropanenitrile (140 μL) and triphenylphosphine (460 mg) in THF (6 mL) is added dropwise DBAD (360 μL). The mixture is stirred for 45 minutes. 2-Hydroxy-2-methylpropanenitrile (140 μL), triphenylphosphine (460 mg) and DBAD (360 μL) are added successively and the mixture is stirred again for 45 minutes. Then the mixture is diluted with THF and purified by HPLC on reversed phase (ACN, water) to give the title compound. LC (Method 2): t.sub.R=0.97 min; Mass spectrum (ESI+): m/z=352 [M+H].sup.+.

Intermediate 30

Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-(1-cyanocyclopropyl)pyridin-3-yl)methyl]-1H-pyrazole-4-carboxylate

[0390] ##STR00434##

[0391] A mixture of ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-(cyanomethyl)pyridin-3-yl)methyl]-1H-pyrazole-4-carboxylate (212 mg) in DMSO (7 mL) is cooled to 10° C. and treated portionwise with NaH (60% in mineral oil, 60 mg). The mixture is stirred for 15 minutes at rt, cooled to 0° C. and treated with 1,2-dibromoethane (80 μL). Then the mixture is stirred for 1 h at rt. After cooling to 0° C. the mixture is treated with saturated aqueous NH.sub.4Cl. The mixture is then extracted twice with EtOAc. The combined organic phases are washed with water, dried (MgSO.sub.4), concentrated and the residue is chromatographed on silica gel (petroleum ether/EtOAc 90:10.fwdarw.60:40) to give the title compound.

[0392] LC (Method 2): t.sub.R=1.06 min; Mass spectrum (ESI+): m/z=378 [M+H].sup.+.

[0393] Intermediate 30-1 is prepared in analogy to Intermediate 30:

TABLE-US-00056 Mass spectrum (ESI+): LC Intermediate Structure t.sub.R m/z [M + H].sup.+ Method 30-1 [00435] embedded image 1.16 380 Method 1

TABLE-US-00057 Intermediate Reaction comment 30-1 The reaction is conducted in DMF. CH.sub.3I is used instead of 1,2-dibromoethane.

TABLE-US-00058 Intermediate Name Name of Starting Material 30-1 Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- (1-cyano-1-methylethyl)pyridin-3-yl)methyl]- (cyanomethyl)pyridin-3-yl)methyl]-1H- 1H-pyrazole-4-carboxylate pyrazole-4-carboxylate

Intermediate 31

Ethyl 1-[(2-{3-azabicyclo[3.1.0]hexan-3-yl}-4-[(1E)-2-phenylethenyl]pyrimidin-5-yl)methyl]-1H-pyrazole-4-carboxylate

[0394] ##STR00436##

[0395] SOCl.sub.2 (5 mL) is added under argon atmosphere to a mixture of (2-{3-azabicyclo[3.1.0]hexan-3-yl}-4-[(1E)-2-phenylethenyl]pyrimidin-5-yl)methanol (1.89 g) in toluene (20 mL). The mixture is heated to 60° C. for 3 h, cooled to rt and concentrated in vacuo. The residue is taken up in DCM (20 mL) and added dropwise to a mixture of ethyl 1H-pyrazole-4-carboxylate (950 mg) and DIPEA (2.2 mL) in DCM (20 mL). After stirring for 12 h at rt the mixture is partitioned between water and DCM. The organic phase is washed with brine, dried (MgSO.sub.4), concentrated and the residue is chromatographed on silica gel (petroleum ether/EtOAc 50:50.fwdarw.0:100) to give the title compound.

[0396] LC (Method 2): t.sub.R=1.06 min; Mass spectrum (ESI+): m/z=416 [M+H].sup.+.

Intermediate 32

1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(methoxymethyl)pyridin-3-yl)methyl]-1H-pyrazole-4-carboxylic acid

[0397] ##STR00437##

[0398] To a solution of ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-(hydroxymethyl)pyridin-3-yl)methyl]-1H-pyrazole-4-carboxylate (900 mg) in DMF (10 mL) is added at 0° C. NaH (60% in mineral oil, 263 mg). The mixture is stirred for 30 minutes, treated with CH.sub.3I (222 μL) and stirred for 1.5 h at 0° C. EtOH (4 mL) and aqueous NaOH (4 M, 4.2 mL) are added and the mixture is stirred for 12 h at 70° C. After cooling to rt aqueous HCl (4 M, 3 mL) is added and the mixture is partitioned between water and EtOAc. The aqueous phase is extracted with EtOAc. The combined organic phases are washed with brine, dried (MgSO.sub.4), concentrated and the residue is purified by HPLC on reversed phase (ACN, water) to give the title compound. LC (Method 2): t.sub.R=0.62 min; Mass spectrum (ESI+): m/z=329 [M+H].sup.+.

[0399] Intermediates 32-1 to 32-3 are prepared in analogy to Intermediate 32:

TABLE-US-00059 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M + H].sup.+ LC Method 32-1 [00438] embedded image 0.63 329 Method 1 32-2 [00439] 0.63 329 Method 1 32-3 [00440] 0.85 and 0.86 459 Method 2 [00441] (mixture of isomers)

TABLE-US-00060 Intermediate Reaction comment 32-3 After addition of CH.sub.3I the mixture is stirred for 12 h at rt. Then water is added and the mixture is purified by HPLC on reversed phase (ACN, water) to give the title compounds as a mixture of isomers.

TABLE-US-00061 Intermediate Name Name of Starting Material 32-1 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- (methoxymethyl)pyridin-3-yl)methyl]-1H- (hydroxymethyl)pyridin-3-yl)methyl]-1H- imidazole-4-carboxylic acid imidazole-4-carboxylate 32-2 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- (methoxymethyl)pyridin-3-yl)methyl]-1H- (hydroxymethyl)pyridin-3-yl)methyl]-1H- pyrazole-4-carboxylic acid pyrazole-4-carboxylate trifluoroacetate 32-3 3-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- (methoxymethyl)pyridin-3-yl)methyl]-1-{[2- (hydroxymethyl)pyridin-3-yl)methyl]-1-{[2- (trimethylsilyl)ethoxy]methyl}-1H-pyrazole-5- (trimethylsilyl)ethoxy]methyl}-1H-pyrazole-5- carboxylic acid carboxylate and and 5-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 5-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- (methoxymethyl)pyridin-3-yl)methyl]-1-{[2- (hydroxymethyl)pyridin-3-yl)methyl]-1-{[2- (trimethylsilyl)ethoxy]methyl}-1H-pyrazole-3- (trimethylsilyl)ethoxy]methyl}-1H-pyrazole-3- carboxylic acid carboxylate (mixture of isomers) (mixture of isomers)

Intermediate 33

Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2-ethylpyridin-3-yl)methyl]-1H-pyrazole-4-carboxylate

[0400] ##STR00442##

[0401] To a ice-cooled solution of (6-{5-azaspiro[2.3]hexan-5-yl}-2-ethylpyridin-3-yl)methanol (400 mg), ethyl 1H-pyrazole-4-carboxylate (800 mg) and tributylphosphine (1.6 mL) in THF (10 mL) is added dropwise DBAD (1.35 g). The mixture is stirred for 45 minutes. Saturated aqueous NaHCO.sub.3 is added and the mixture is stirred vigorously for 5 minutes. Then the mixture is filtered over celite. The aqueous phase is extracted twice with EtOAc and the combined organic phases are washed with brine and dried (MgSO.sub.4). The solvents are evaporated in vacuo and the residue is chromatographed on silica gel (petroleum ether/EtOAc 70:30.fwdarw.0:100) to give the title compound.

[0402] LC (Method 2): t.sub.R=0.78 min; Mass spectrum (ESI+): m/z=341 [M+H].sup.+.

[0403] Intermediates 33-1 to 33-5 are prepared in analogy to Intermediate 33:

TABLE-US-00062 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M + H].sup.+ LC Method 33-1 [00443] embedded image 1.14 405 Method 1 33-2 [00444] 0.73 327 Method 2 33-3 [00445] 0.65 364 Method 1 33-4 [00446] 0.84 401 Method 1 33-5 [00447] 0.77 365 Method 1

TABLE-US-00063 Intermediate Reaction comment 33-1 DBAD is added to the reaction mixture at 0° C. The mixture is stirred for 12 h while warming to rt. 33-4 DBAD is added to the reaction mixture at 0° C. The mixture is stirred for 12 h while warming to rt. 33-5 DBAD is added to the reaction mixture at 0° C. The mixture is stirred for 12 h while warming to rt.

TABLE-US-00064 Name of Name of Intermediate Name Starting Material 1 Starting Material 2 33-1 Ethyl 1-[(6-{3-azabicyclo[3.1.0]-hexan- (6-{3-Azabicyclo[3.1.0]- Ethyl 3-bromo-1H- 3-yl}-2-methylpyridin-3-yl)methyl]-3- hexan-3-yl}-2-methylpyridin- pyrazole-4-carboxylate bromo-1H-pyrazole-4-carboxylate 3-yl)methanol 33-2 Ethyl 1-[(6-{3-azabicyclo[3.1.0]-hexan- (6-{3-Azabicyclo[3.1.0]- Ethyl 1H-pyrazole-4- 3-yl}-2-methylpyridin-3-yl)methyl]-1H- hexan-3-yl}-2-methylpyridin- carboxylate pyrazole-4-carboxylate 3-yl)methanol 33-3 Methyl 7-[(6-{3-azabicyclo[3.1.0]- (6-{3-Azabicyclo[3.1.0]- Methyl 7H-pyrrolo[2,3- hexan-3-yl}-2-methylpyridin-3-yl)- hexan-3-yl}-2-methyl- d]pyrimidine-5- methyl]-7H-pyrrolo[2,3-d]pyrimidine-5- pyridin-3-yl)methanol carboxylate carboxylate 33-4 Methyl 7-[(2-{6,6-difluoro-3- (2-{6,6-Difluoro-3- Methyl 7H-pyrrolo[2,3- azabicyclo[3.1.0]hexan-3-yl}-4- azabicyclo[3.1.0]hexan-3- d]pyrimidine-5- methylpyrimidin-5-yl)methyl]-7H- yl}-4-methylpyrimidin-5- carboxylate pyrrolo[2,3-d]pyrimidine-5-carboxylate yl)methanol 33-5 Methyl 7-[(2-{3-azabicyclo[3.1.0]- (2-{3-Azabicyclo[3.1.0]- Methyl 7H-pyrrolo[2,3- hexan-3-yl}-4-methylpyrimidin-5-yl)- hexan-3-yl}-4-methyl- d]pyrimidine-5- methyl]-7H-pyrrolo[2,3-d]pyrimidine-5- pyrimidin-5-yl)methanol carboxylate carboxylate

Intermediate 34

Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-[(1E)-3-(benzyloxy)prop-1-en-1-yl]pyridin-3-yl)methyl]-1H-pyrazole-4-carboxylate

[0404] ##STR00448##

[0405] In a microwave vial a mixture of ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-chloropyridin-3-yl)methyl]-1H-pyrazole-4-carboxylate (100 mg), potassium (E)-3-(benzyloxy)prop-1-enyltrifluoroborate (88 mg), K.sub.2CO.sub.3 (100 mg) and THF (5 mL) is purged for 10 minutes with argon. 1,1′-Bis(diphenylphosphino)ferrocenepalladium(II) dichloride (Pd(dppf)Cl.sub.2, 15 mg) is added, the vial is sealed and the mixture is heated to 80° C. for 15 h. After cooling to rt the mixture is partitioned between water and EtOAc. The aqueous phase is extracted with EtOAc. The combined organic phases are washed with brine, dried (MgSO.sub.4), concentrated in vacuo and the residue is chromatographed on silica gel (petroleum ether/EtOAc 70:30.fwdarw.0:100) to give the title compound.

[0406] LC (Method 2): t.sub.R=0.95 min; Mass spectrum (ESI+): m/z=459 [M+H].sup.+.

Intermediate 35

Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-(3-hydroxypropyl)pyridin-3-yl)methyl]-1H-pyrazole-4-carboxylate

[0407] ##STR00449##

[0408] A mixture of ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-[(1E)-3-(benzyloxy)prop-1-en-1-yl]pyridin-3-yl)methyl]-1H-pyrazole-4-carboxylate (60 mg), 10% palladium on carbon (6 mg) in THF (2 mL) and acetic acid (8 μL) is shaken under hydrogen atmosphere (3 bar) at rt for 12 h. The mixture is filtered, the filtrate is concentrated and the residue is chromatographed on silica gel (petroleum ether/EtOAc 70:30.fwdarw.0:100) to give the title compound.

[0409] LC (Method 2): t.sub.R=0.73 min; Mass spectrum (ESI+): m/z=371 [M+H].sup.+.

Intermediate 36

3-[5-(Azidomethyl)-6-ethenylpyridin-2-yl]-6,6-difluoro-3-azabicyclo[3.1.0]hexane

[0410] ##STR00450##

[0411] Under argon atmosphere diphenylphosphorylazide (1.4 mL) is added dropwise to an ice-cooled mixture of (6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-ethenylpyridin-3-yl)methanol (1.34 g) and DBU (1.05 mL) in toluene (10 mL) and ACN (10 mL). The mixture is stirred for 12 h while warming to rt. Then the mixture is partitioned between water and EtOAc. The aqueous phase is extracted with EtOAc. The combined organic phases are washed with brine, dried (MgSO.sub.4), concentrated in vacuo and the residue is chromatographed on silica gel (petroleum ether/EtOAc 99:1.fwdarw.50:50) to give the title compound. LC (Method 2): t.sub.R=0.88 min; Mass spectrum (ESI+): m/z=278 [M+H].sup.+.

[0412] Intermediates 36-1 to 36-5 are prepared in analogy to Intermediate 36:

TABLE-US-00065 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M + H].sup.+ LC Method 36-1 [00451] embedded image 0.69 230 Method 2 36-2 [00452] 0.72 242 Method 2 36-3 [00453] 1.00 267 Method 2 36-4 [00454] 0.97 267 Method 2 36-5 [00455] 0.75 231 Method 2

TABLE-US-00066 Intermediate Reaction comment 36-3 The reaction is conducted in toluene/ACN 1:1. 36-4 The reaction is conducted in toluene/ACN 1:1. 36-5 The reaction is conducted in toluene/ACN 1:1.

TABLE-US-00067 Intermediate Name Name of Starting Material 36-1 5-[5-(Azidomethyl)-6-methylpyridin-2-yl]-5- (6-{5-Azaspiro[2.3]hexan-5-yl}-2- azaspiro[2.3]hexane methylpyridin-3-yl)methanol 36-2 3-[5-(Azidomethyl)-6-ethenylpyridin-2-yl]-3- (6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- azabicyclo[3.1.0]hexane ethenylpyridin-3-yl)methanol 36-3 3-[5-(Azidomethyl)-6-methylpyrazin-2-yl]-6,6- (5-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- difluoro-3-azabicyclo[3.1.0]hexane yl}-3-methylpyrazin-2-yl)methanol 36-4 3-[5-(Azidomethyl)-4-methylpyrimidin-2-yl]- (2-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- 6,6-difluoro-3-azabicyclo[3.1.0]hexane yl}-4-methylpyrimidin-5-yl)methanol 36-5 3-[5-(Azidomethyl)-4-methylpyrimidin-2-yl]-3- (2-{3-Azabicyclo[3.1.0]hexan-3-yl}-4- azabicyclo[3.1.0]hexane methylpyrimidin-5-yl)methanol

Intermediate 37

Ethyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-ethenylpyridin-3-yl)methyl]-1H-1,2,3-triazole-4-carboxylate

[0413] ##STR00456##

[0414] A mixture of 3-[5-(azidomethyl)-6-ethenylpyridin-2-yl]-6,6-difluoro-3-azabicyclo[3.1.0]hexane (794 mg), propiolic acid ethylester (320 μL), CuSO.sub.4 (92 mg) and sodium (L)-ascorbate (568 mg) in tert.-butanol (8 mL) and water (8 mL) is stirred at rt for 48 h. The mixture is partitioned between water and EtOAc. The aqueous phase is extracted with EtOAc. The combined organic phases are washed with brine, dried (MgSO.sub.4), concentrated in vacuo and the residue is chromatographed on silica gel (petroleum ether/EtOAc 99:1.fwdarw.50:50) to give the title compound.

[0415] LC (Method 2): t.sub.R=0.89 min; Mass spectrum (ESI+): m/z=376 [M+H].sup.+.

[0416] Intermediates 37-1 to 37-5 are prepared in analogy to Intermediate 37:

TABLE-US-00068 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M + H].sup.+ LC Method 37-1 [00457] embedded image 0.69 328 Method 2 37-2 [00458] 0.74 340 Method 2 37-3 [00459] 0.95 365 Method 2 37-4 [00460] 0.87 365 Method 2 37-5 [00461] 0.79 329 Method 2

TABLE-US-00069 Intermediate Reaction comment 37-3 The reaction is conducted for 12 h. 37-5 The reaction is conducted for 5 days.

TABLE-US-00070 Intermediate Name Name of Starting Material 37-1 Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2-methyl- 5-[5-(Azidomethyl)-6-methylpyridin-2-yl]- pyridin-3-yl)methyl]-1H-1,2,3-triazole-4-carboxylate 5-azaspiro[2.3]hexane 37-2 Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- 3-[5-(Azidomethyl)-6-ethenylpyridin-2-yl]- ethenylpyridin-3-yl)methyl]-1H-1,2,3-triazole-4- 3-azabicyclo[3.1.0]hexane carboxylate 37-3 Ethyl 1-[(5-{6,6-difluoro-3-azabicyclo[3.1.0]hexan- 3-[5-(Azidomethyl)-6-methylpyrazin-2-yl]- 3-yl}-3-methylpyrazin-2-yl)methyl]-1H-1,2,3- 6,6-difluoro-3-azabicyclo[3.1.0]hexane triazole-4-carboxylate 37-4 Ethyl 1- [(2-{6,6-difluoro-3-azabicyclo[3.1.0]-hexan- 3-[5-(Azidomethyl)-4-methylpyrimidin-2- 3-yl}-4-methylpyrimidin-5-yl)methyl]-1H-1,2,3- yl]-6,6-difluoro-3-azabicyclo[3.1.0]hexane triazole-4-carboxylate 37-5 Ethyl 1-[(2-{3-azabicyclo[3.1.0]hexan-3-yl}-4- 3-[5-(Azidomethyl)-4-methylpyrimidin-2- methylpyrimidin-5-yl)methyl]-1H-1,2,3-triazole-4- yl]-3-azabicyclo[3.1.0]hexane carboxylate

[0417] Intermediate 38

1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-(methoxymethyl)pyridin-3-yl)methyl]-1H-1,2,3-triazole-4-carboxylic acid

[0418] ##STR00462##

[0419] To a solution of ethyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-(hydroxymethyl)pyridin-3-yl)methyl]-1H-1,2,3-triazole-4-carboxylate (175 mg) in DMF (2 mL) is added at 0° C. NaH (60% in mineral oil, 45 mg). The mixture is stirred for 30 minutes at rt, treated with CH.sub.3I (30 μL) and stirred for 12 h at rt. Water is added and the mixture is concentrated in vacuo. The residue is taken up in DCM/isopropanol 1:1 and filtered. The filtrate is dried (MgSO.sub.4) and concentrated in vacuo to give the title compound.

[0420] LC (Method 2): t.sub.R=0.65 min; Mass spectrum (ESI+): m/z=366 [M+H].sup.+.

Intermediate 39

Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-(1-hydroxypropan-2-yl)pyridin-3-yl)methyl]-1H-pyrazole-4-carboxylate

[0421] ##STR00463##

[0422] A mixture of ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-(prop-1-en-2-yl)pyridin-3-yl)methyl]-1H-pyrazole-4-carboxylate (270 mg) and 9-borabicyclo(3.3.1)nonane (3.1 mL) is stirred for 48 h at rt. 9-Borabicyclo(3.3.1)nonane (6 mL) is added and stirring is continued for 12 h. The mixture is cooled to 0° C. and treated dropwise with water (3 mL) and H.sub.2O.sub.2 (35% in water, 3.35 mL). Then the mixture is stirred for 30 minutes at rt. Aqueous NaOH (2 M, 340 μL) is added, the mixture is stirred for 20 minutes and then cooled to 0° C. Saturated aqueous Na.sub.2S.sub.2O.sub.3 is slowly added and the aqueous phase is extracted twice with EtOAc. The combined organic phases are washed with brine, dried (MgSO.sub.4), concentrated in vacuo and the residue is chromatographed on silica gel (petroleum ether/EtOAc 80:20.fwdarw.0:100) to give the title compound. LC (Method 2): t.sub.R=0.76 min; Mass spectrum (ESI+): m/z=371 [M+H].sup.+.

[0423] Intermediate 39-1 is prepared in analogy to Intermediate 39:

TABLE-US-00071 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M + H].sup.+ LC Method 39-1 [00464] embedded image 0.98 357 Method 1

TABLE-US-00072 Intermediate Reaction comment 39-1 The hydroboration is conducted for 3 h at rt.

TABLE-US-00073 Intermediate Name Name of Starting Material 39-1 Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- (2-hydroxyethyl)pyridin-3-yl)methyl]-1H- ethenylpyridin-3-yl)methyl]-1H-pyrazole-4- pyrazole-4-carboxylate carboxylate

Intermediate 40

Methyl 6-{3-azabicyclo[3.1.0]hexan-3-yl}pyridazine-3-carboxylate

[0424] ##STR00465##

[0425] Under argon atmosphere a mixture of methyl 6-chloropyridazine-3-carboxylate (2.5 g), 3-azabicyclo[3.1.0]hexane hydrochloride (1.99 g) and K.sub.2CO.sub.3 (4.02 g) in DMF (50 mL) is stirred for 12 h at rt. The mixture is partitioned between water and EtOAc and stirred for 20 minutes. The precipitate is collected by filtration and dried in vacuo to give the title compound. LC (Method 2): t.sub.R=0.60 min; Mass spectrum (ESI+): m/z=220 [M+H].sup.+.

[0426] Intermediates 40-1 to 40-8 are prepared in analogy to Intermediate 40:

TABLE-US-00074 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M + H].sup.+ LC Method 40-1 [00466] embedded image 0.68 219 Method 2 40-2 [00467] 0.96 219 Method 1 40-3 [00468] 0.93 254 Method 2 40-4 [00469] 0.93 270 Method 2 40-5 [00470] 1.07 287 Method 1 40-6 [00471] 1.02 284 Method 2 40-7 [00472] 1.02 248 Method 2 40-8 [00473] 1.00 248 Method 1

TABLE-US-00075 Intermediate Reaction comment 40-1 The reaction is conducted at 90° C. for 12 h. 40-3 The reaction is conducted for 48 h at rt. 40-4 The reaction is conducted at 90° C. for 12 h. 40-5 KHCO.sub.3 is used instead of K.sub.2CO.sub.3 and DMSO instead of DMF. The reaction is conducted at 45° C. for 4 h. 40-6 The reaction is conducted at 90° C. for 1 h. 40-7 The reaction is conducted at 90° C. for 1 h.

TABLE-US-00076 Name of Name of Intermediate Name Starting Material 1 Starting Material 2 40-1 Methyl 6-{3-azabicyclo[3.1.0]- Methyl 6-chloropyridine-3- 3-Azabicyclo[3.1.0]hexane hexan-3-yl}pyridine-3-carboxylate carboxylate hydrochloride 40-2 Methyl 6-{5-azaspiro[2.3]hexan- Methyl 6-fluororopyridine-3- 5-Azaspiro[2.3]hexane 5-yl}pyridine-3-carboxylate carboxylate trifluoroacetate 40-3 Methyl 5-{3-azabicyclo[3.1.0]- Methyl 3,5-dichloropyrazine- 3-Azabicyclo[3.1.0]hexane hexan-3-yl}-3-chloropyrazine-2- 2-carboxylate hydrochloride carboxylate 40-4 Methyl 5-{6,6-difluoro-3- Methyl 5-chloro-3- 6,6-Difluoro-3- azabicyclo[3.1.0]hexan-3-yl}-3- methylpyrazine-2- azabicyclo[3.1.0]hexane methylpyrazine-2-carboxylate carboxylate hydrochloride 40-5 Methyl 6-{3-azabicyclo[3.1.0]- Methyl 6-chloro-4- 3-Azabicyclo[3.1.0]hexane hexan-3-yl}-4-(trifluoromethyl)- (trifluoromethyl)pyridine-3- hydrochloride pyridine-3-carboxylate carboxylate 40-6 Ethyl 2-{6,6-difluoro-3- Ethyl 2-chloro-4- 6,6-Difluoro-3- azabicyclo[3.1.0]hexan-3-yl}-4- methylpyrimidine-5- azabicyclo[3.1.0]hexane methylpyrimidine-5-carboxylate carboxylate hydrochloride 40-7 Ethyl 2-{3- Ethyl 2-chloro-4- 3-Azabicyclo[3.1.0]hexane azabicyclo[3.1.0]hexan-3-yl}-4- methylpyrimidine-5- hydrochloride methylpyrimidine-5-carboxylate carboxylate 40-8 Ethyl 2-{5-azaspiro[2.3]hexan-5- Ethyl 2-chloro-4- 5-azaspiro[2.3]hexane yl}-4-methylpyrimidine-5- methylpyrimidine-5- trifluoroacetate carboxylate carboxylate

Intermediate 41

(6-{3-Azabicyclo[3.1.0]hexan-3-yl}pyridazin-3-yl)methanol

[0427] ##STR00474##

[0428] NaBH.sub.4 (76 mg) is added portionwise to a mixture of methyl 6-{3-azabicyclo[3.1.0]hexan-3-yl}pyridazine-3-carboxylate (200 mg) and CaCl.sub.2 (54 mg) in MeOH (4 mL). The mixture is stirred for 24 h at 70° C. After cooling to rt 1 M aqueous HCl is added until a pH-value of 2 is reached. The mixture is stirred for 15 minutes and then partitioned between saturated aqueous NaHCO.sub.3 and EtOAc. The aqueous phase is extracted twice with EtOAc and twice with EtOAc/isopropanol 1:1. The combined organic phases are dried (MgSO.sub.4), concentrated in vacuo and the residue is purified by HPLC on reversed phase (ACN, water) to give the title compound.

[0429] LC (Method 1): t.sub.R=0.67 min; Mass spectrum (ESI+): m/z=192 [M+H].sup.+.

[0430] Intermediate 41-1 is prepared in analogy to Intermediate 41:

TABLE-US-00077 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M + H].sup.+ LC Method 41-1 [00475] embedded image 0.67 379 Method 2

TABLE-US-00078 Intermediate Reaction comment 41-1 The reaction is conducted in THF/EtOH 1:1 for 8 h at rt.

TABLE-US-00079 Intermediate Name Name of Starting Material 41-1 Ethyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- Ethyl 6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan- hexan-3-yl}-2-(hydroxymethyl)pyridin-3- 3-yl}-3-{[4-(ethoxycarbonyl)-1H-imidazol-1- yl)methyl]-1H-imidazole-4-carboxylate yl]methyl}pyridine-2-carboxylate

Intermediate 42

Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}pyridazin-3-yl)methyl]-1H-pyrazole-4-carboxylate

[0431] ##STR00476##

[0432] To a solution of (6-{3-azabicyclo[3.1.0]hexan-3-yl}pyridazin-3-yl)methanol (105 mg), ethyl 1H-pyrazole-4-carboxylate (81 mg) and triphenylphosphine (166 mg) in THF (2 mL) is added DBAD (139 mg). The mixture is stirred for 1.5 h, diluted with DMF and purified by HPLC on reversed phase (ACN, water) to give the title compound.

[0433] LC (Method 2): t.sub.R=0.68 min; Mass spectrum (ESI+): m/z=314 [M+H].sup.+.

[0434] Intermediates 42-1 to 42-2 are prepared in analogy to Intermediate 42:

TABLE-US-00080 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M + H].sup.+ LC Method 42-1 [00477] embedded image 1.04 348 Method 2 42-2 [00478] 0.89 281 Method 2

TABLE-US-00081 Intermediate Reaction comment 42-1 The reaction mixture is chromatographed on silica gel (petroleum ether/EtOAc 95:5 .fwdarw. 45:55) to give the title compound. 42-2 The reaction mixture is stirred for 12 h at rt. The crude product is chromatographed on silica gel (cyclohexane/ EtOAc 50:50 .fwdarw. 0:100) to give the title compound.

TABLE-US-00082 Name of Name of Intermediate Name Starting Material 1 Starting Material 2 42-1 Ethyl 1-[(5-{3-azabicyclo[3.1.0]-hexan-3- (5-{3-Azabicyclo[3.1.0]-hexan-3- Ethyl 1H-pyrazole- yl}-3-chloropyrazin-2-yl)methyl]-1H- yl}-3-chloropyrazin-2-yl)methanol 4-carboxylate pyrazole-4-carboxylate 42-2 Ethyl 1-[(2-chloro-4-methyl-pyrimidin-5- (2-Chloro-4-methyl-pyrimidin-5- Ethyl 1H-pyrazole- yl)methyl]-1H-pyrazole-4-carboxylate yl)methanol 4-carboxylate

Intermediate 43

Ethyl 1-[(5-bromopyrimidin-2-yl)methyl]-1H-pyrazole-4-carboxylate

[0435] ##STR00479##

[0436] A mixture of 5-bromo-2-(bromomethyl)pyrimidine (1.5 g), K.sub.2CO.sub.3 (2.4 g) and ethyl 1H-pyrazole-4-carboxylate (814 mg) in DMF (20 mL) is stirred for 1.5 h at rt. The mixture is diluted with THF and filtered overcelite. The filter cake is washed twice with THF. The combined filtrates are concentrated and the residue is chromatographed on silica gel (petroleum ether/EtOAc 80:20.fwdarw.0:100) to give the title compound.

[0437] LC (Method 1): t.sub.R=0.85 min; Mass spectrum (ESI+): m/z=311 [M+H].sup.+.

Intermediate 44

Ethyl 1-[(5-{3-azabicyclo[3.1.0]hexan-3-yl}pyrimidin-2-yl)methyl]-1H-pyrazole-4-carboxylate

[0438] ##STR00480##

[0439] In a microwave vial a mixture of ethyl 1-[(5-bromopyrimidin-2-yl)methyl]-1H-pyrazole-4-carboxylate (500 mg), 3-azabicyclo[3.1.0]hexane hydrochloride (384 mg), Cs.sub.2CO.sub.3 (1.6 g), tris(dibenzylideneacetone)dipalladium(0) (Pd.sub.2dba.sub.3, 74 mg) and 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (Xantphos, 93 mg) in DMF (1.3 mL) and toluene (3.8 mL) is purged for 10 minutes with argon. The vial is sealed and the mixture is heated to 90° C. for 2 h. Then the mixture is partitioned between water and EtOAc. The aqueous phase is extracted with EtOAc. The combined organic phases are dried (MgSO.sub.4), concentrated in vacuo and the residue is chromatographed on silica gel (petroleum ether/EtOAc 90:10.fwdarw.20:80) to give the title compound. LC (Method 1): t.sub.R=0.91 min; Mass spectrum (ESI+): m/z=314 [M+H].sup.+.

Intermediate 45

(6-{3-Azabicyclo[3.1.0]hexan-3-yl}pyridin-3-yl)methanol

[0440] ##STR00481##

[0441] A mixture of methyl 6-{3-azabicyclo[3.1.0]hexan-3-yl}pyridine-3-carboxylate (8 g) and LiBH.sub.4 (2 M solution in THF, 20 mL) in THF (60 mL) and MeOH (3 mL) is stirred for 12 h at 60° C. LiBH.sub.4 (2 M solution in THF, 5 mL) is added and the mixture is stirred for 2 h at 60° C. Then the mixture is cooled to 0° C. and carefully treated with water. The mixture is concentrated and the residue is partitioned between water and EtOAc. The organic phase is dried (MgSO.sub.4), concentrated in vacuo and the residue is chromatographed on silica gel (DCM/MeOH 0:100.fwdarw.90:10) to give the title compound. LC (Method 1): t.sub.R=0.79 min; Mass spectrum (ESI+): m/z=191 [M+H].sup.+.

[0442] Intermediates 45-1 to 45-3 are prepared in analogy to Intermediate 45:

TABLE-US-00083 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M + H].sup.+ LC Method 45-1 [00482] embedded image 0.80 191 Method 1 45-2 [00483] 0.71 242 Method 2 45-3 [00484] 0.62 242 Method 2

TABLE-US-00084 Intermediate Reaction comment 45-2 The reaction is conducted in 1,4-dioxane/MeOH 15:1 for 3 h at rt. 45-3 The reaction is conducted for 12 h at 50° C.

TABLE-US-00085 Intermediate Name Name of Starting Material 45-1 (6-{5-Azaspiro[2.3]hexan-5-yl}pyridin-3-yl)- Methyl 6-{5-azaspiro[2.3]hexan-5-yl}pyridine- methanol 3-carboxylate 45-2 (5-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}- Methyl 5-{6,6-difluoro-3-azabicyclo[3.1.0]- 3-methylpyrazin-2-yl)methanol hexan-3-yl}-3-methylpyrazine-2-carboxylate 45-3 (2-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}- Ethyl 2-{6,6-difluoro-3-azabicyclo[3.1.0]- 4-methylpyrimidin-5-yl)methanol hexan-3-yl}-4-methylpyrimidine-5-carboxylate

Intermediate 46

1-[(6-{5-Azaspiro[2.3]hexan-5-yl}pyridin-3-yl)methyl]-1H-pyrazole-4-carboxylic acid trifluoroacetate

[0443] ##STR00485##

[0444] To an ice-cooled solution of (6-{5-azaspiro[2.3]hexan-5-yl}pyridin-3-yl)methanol (170 mg), ethyl 1H-pyrazole-4-carboxylate (130 mg) and tributylphosphine (450 μL) in THF (5 mL) is added dropwise DBAD (338 mg). The mixture is stirred for 48 h. Then the mixture is concentrated in vacuo. The residue is taken up in MeOH (10 mL) and aqueous NaOH (1 M, 5 mL) and stirred for 2 h at rt. After neutralization with trifluoroacetic acid the crude product is purified by HPLC on reversed phase (ACN, water) to give the title compound.

[0445] LC (Method 1): t.sub.R=0.65 min; Mass spectrum (ESI+): m/z=285 [M+H].sup.+.

Intermediate 47

1-[(6-{5-Azaspiro[2.3]hexan-5-yl}pyridin-3-yl)methyl]-1H-imidazole-4-carboxylic acid trifluoroacetate

[0446] ##STR00486##

[0447] In a microwave vial a mixture of (6-{5-azaspiro[2.3]hexan-5-yl}pyridin-3-yl)methanol (85 mg), ethyl 1H-imidazole-4-carboxylate (76 mg) and p-toluenesulfonic acid (90 mg) in ACN (4 mL) is heated for 48 h to 75° C., for 3 h to 90° C., for 3 h to 100° C. and for 12 h to 80° C. After cooling to rt the mixture is diluted with ACN and purified by HPLC on reversed phase (ACN, water). The product thus obtained is dissolved in MeOH (2 mL) and aqueous NaOH (1 M, 500 μL). After stirring for 2 h at rt the mixture is neutralized with trifluoroacetic and purified by HPLC on reversed phase (ACN, water) to give the title compound. LC (Method 2): t.sub.R=0.40 min; Mass spectrum (ESI+): m/z=285 [M+H].sup.+.

Intermediate 48

1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-formylpyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]-1H-1,2,3-triazole-4-carboxamide

[0448] ##STR00487##

[0449] A mixture of 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-(hydroxymethyl)pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]-1H-1,2,3-triazole-4-carboxamide (19 mg) and 1,1,1-tris(acetyloxy)-1,1-dihydro-1,2-benziodoxol-3-(1H)-one (Dess-Martin periodinane, 26 mg) in DCM (2 mL) is stirred for 3 h at rt. The mixture is partitioned between saturated aqueous NaHCO.sub.3 and DCM. The aqueous phase is extracted with DCM for three times. The combined organic phases are dried (Na.sub.2SO.sub.4) and concentrated in vacuo to give the title compound.

[0450] LC (Method 1): t.sub.R=0.89 min; Mass spectrum (ESI+): m/z=469 [M+H].sup.+.

[0451] Intermediate 48-1 is prepared in analogy to Intermediate 48:

TABLE-US-00086 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M + H].sup.+ LC Method 48-1 [00488] embedded image 1.10 355 Method 2

TABLE-US-00087 Intermediate Reaction comment 48-1 The crude product is chromatographed on silica gel (petroleum ether/EtOAc 95:5 .fwdarw. 70:30) to give the title compound.

TABLE-US-00088 Intermediate Name Name of Starting Material 48-1 Ethyl 1-[(2-acetyl-6-{3-azabicyclo[3.1.0]hexan- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- 3-yl}pyridin-3-yl)methyl]-1H-pyrazole-4- (1-hydroxyethyl)pyridin-3-yl)methyl]-1H- carboxylate pyrazole-4-carboxylate

Intermediate 49

Ethyl 6-{5-azaspiro[2.3]hexan-5-yl}-3-{[4-(ethoxycarbonyl)-1H-imidazol-1-yl]methyl}pyridine-2-carboxylate

[0452] ##STR00489##

[0453] A mixture of ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2-chloropyridin-3-yl)methyl]-1H-imidazole-4-carboxylate (860 mg), bis(triphenylphosphine)palladium(II) dichloride (Pd(PPh.sub.3).sub.2Cl.sub.2, 200 mg) and triethylamine (1.1 mL) in EtOH (60 mL) is heated under a carbonmonoxide atmosphere of 10 bar to 130° C. for 5 h. The solvents are evaporated in vacuo and the residue is purified by HPLC on reversed phase (ACN, water) to give the title compound.

[0454] LC (Method 2): t.sub.R=0.89 min; Mass spectrum (ESI+): m/z=385 [M+H].sup.+.

[0455] Intermediates 49-1 to 49-4 are prepared in analogy to Intermediate 49:

TABLE-US-00089 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M + H].sup.+ LC Method 49-1 [00490] embedded image 1.03 385 Method 2 49-2 [00491] 0.89 385 Method 2 49-3 [00492] 0.89 421 Method 2 49-4 [00493] 0.91 385 Method 2

TABLE-US-00090 Intermediate Reaction comment 49-3 The reaction is conducted under a carbonmonoxide atmosphere of 5 bar at 90° C. 49-4 The reaction is conducted under a carbonmonoxide atmosphere of 4 bar at 100° C. for 14 h.

TABLE-US-00091 Intermediate Name Name of Starting Material 49-1 Ethyl 6-{5-azaspiro[2.3]hexan-5-yl}-3-{[4- Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- (ethoxycarbonyl)-1H-pyrazol-1- chloropyridin-3-yl)methyl]-1H-pyrazole-4- yl]methyl}pyridine-2-carboxylate carboxylate 49-2 Ethyl 6-{5-azaspiro[2.3]hexan-5-yl}-3-{[4- Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- (ethoxycarbonyl)-1H-imidazol-1- chloropyridin-3-yl)methyl]-1H-imidazole-4- yl]methyl}pyridine-2-carboxylate carboxylate 49-3 Ethyl 6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan- Ethyl 1-[(2-bromo-6-{6,6-difluoro-3- 3-yl}-3-{[4-(ethoxycarbonyl)-1H-imidazol-1- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- yl]methyl}pyridine-2-carboxylate yl)methyl]-1H-imidazole-4-carboxylate 49-4 Ethyl 6-{3-azabicyclo[3.1.0]hexan-3-yl}-3-{[4- Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- (ethoxycarbonyl)-1H-imidazol-1- bromopyridin-3-yl)methyl]-1H-imidazole-4- yl]methyl}pyridine-2-carboxylate carboxylate

Intermediate 50

Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2-(hydroxymethyl)pyridin-3-yl)methyl]-1H-imidazole-4-carboxylate bistrifluoroacetate

[0456] ##STR00494##

[0457] LiBH.sub.4 (150 mg) is added portionwise to a mixture of ethyl 6-{5-azaspiro[2.3]hexan-5-yl}-3-{[4-(ethoxycarbonyl)-1H-imidazol-1-yl]methyl}pyridine-2-carboxylate (705 mg) in THF (15 mL). The mixture is stirred for 2 h at rt, cooled to 0° C., treated with aqueous HCl (4 M, 2 mL) and purified by HPLC on reversed phase (ACN, water) to give the title compound.

[0458] LC (Method 1): t.sub.R=0.91 min; Mass spectrum (ESI+): m/z=343 [M+H].sup.+.

[0459] Intermediates 50-1 to 50-2 are prepared in analogy to Intermediate 50:

TABLE-US-00092 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M + H].sup.+ LC Method 50-1 [00495] embedded image 1.00 343 Method 1 50-2 [00496] 0.91 343 Method 1

TABLE-US-00093 Intermediate Name Name of Starting Material 50-1 Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- Ethyl 6-{5-azaspiro[2.3]hexan-5-yl}-3-{[4- (hydroxymethyl)pyridin-3-yl)methyl]-1H- (ethoxycarbonyl)-1H-pyrazol-1- pyrazole-4-carboxylate trifluoroacetate yl]methyl}pyridine-2-carboxylate 50-2 Ethyl 1-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- Ethyl 6-{5-azaspiro[2.3]hexan-5-yl}-3-{[4- (hydroxymethyl)pyridin-3-yl)methyl]-1H- (ethoxycarbonyl)-1H-imidazol-1- imidazole-4-carboxylate yl]methyl}pyridine-2-carboxylate

Intermediate 51

1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}pyridin-3-yl)methyl]-1H-pyrazole-4-carboxylic acid trifluoroacetate

[0460] ##STR00497##

[0461] To an ice-cooled solution of (6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}pyridin-3-yl)methanol (158 mg), ethyl 1H-pyrazole-4-carboxylate (100 mg) and tributylphosphine (210 μL) in THF (2 mL) is added dropwise DBAD (190 mg). The mixture is stirred for 30 minutes and is then treated with aqueous NaOH (4 M, 750 μL). After stirring for 12 h at rt, aqueous HCl (4 M, 750 μL) is added and the mixture is purified by HPLC on reversed phase (ACN, water) to give the title compound. LC (Method 2): t.sub.R=0.63 min; Mass spectrum (ESI+): m/z=321 [M+H].sup.+.

Intermediate 52

Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-(1-hydroxyethyl)pyridin-3-yl)methyl]-1H-pyrazole-4-carboxylate

[0462] ##STR00498##

[0463] CH.sub.3MgBr (3 M in diethylether, 1.22 mL) is added dropwise at −40° C. to a mixture of ethyl 1-[(6-{3-azabicyclo[3.1.0]-hexan-3-yl}-2-formylpyridin-3-yl)methyl]-1H-pyrazole-4-carboxylate (1 g) in THF (20 mL). The mixture is stirred for 25 minutes while warming to −25° C. Aqueous HCl (1 M, 4 mL) is added. After stirring for 5 minutes the mixture is partitioned between saturated aqueous NaHCO.sub.3 and EtOAc. The aqueous phase is extracted with EtOAc. The combined organic phases are washed with brine, dried (MgSO.sub.4) and concentrated in vacuo to give the title compound.

[0464] LC (Method 2): t.sub.R=0.73 min; Mass spectrum (ESI+): m/z=357 [M+H].sup.+.

[0465] Intermediate 52-1 is prepared in analogy to Intermediate 52:

TABLE-US-00094 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M + H].sup.+ LC Method 52-1 [00499] embedded image 1.10 371 Method 1

TABLE-US-00095 Intermediate Reaction comment 52-1 The reaction is conducted at −10° C. Intermediate Name Name of Starting Material 52-1 Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(2-acetyl-6-{3-azabicyclo[3.1.0]hexan- (2-hydroxypropan-2-yl)pyridin-3-yl)methyl]-1H- 3-yl}pyridin-3-yl)methyl]-1H-pyrazole-4- pyrazole-4-carboxylate carboxylate

Intermediate 53

Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-[(1R)-1-hydroxyethyl]pyridin-3-yl)methyl]-1H-pyrazole-4-carboxylate

[0466] ##STR00500##

[0467] Triethylamine (246 μL) is dissolved in DCM (3 mL), cooled to 0° C. and treated successively with formic acid (75 μL), ethyl 1-[(2-acetyl-6-{3-azabicyclo[3.1.0]hexan-3-yl}pyridin-3-yl)methyl]-1H-pyrazole-4-carboxylate (200 mg) and chloro{[(1R,2R)-(−)-2-amino-1,2-diphenylethyl](4-toluenesulfonyl)amido}(mesitylene)ruthenium(II) (RuCl[(R,R)-Tsdpen(mesitylene), 23 mg]. The mixture is stirred for 24 h while warming to rt. The mixture is concentrated, taken up in THF (5 mL), treated with a solution of NH.sub.3 in MeOH (7 M, 1 mL), and water (1 mL). Then the mixture is purified by HPLC on reversed phase (ACN, water) to give the title compound.

[0468] LC (Method 1): t.sub.R=1.03 min; Mass spectrum (ESI+): m/z=357 [M+H].sup.+.

[0469] Intermediate 53-1 is prepared in analogy to Intermediate 53:

TABLE-US-00096 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M + H].sup.+ LC Method 53-1 [00501] embedded image 1.03 357 Method 1

TABLE-US-00097 Intermediate Reaction comment 53-1 RuCl[(S,S)-Tsdpen(mesitylene) is used instead of RuCl[(R,R)-Tsdpen(mesitylene).

TABLE-US-00098 Intermediate Name Name of Starting Material 53-1 Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(2-acetyl-6-{3-azabicyclo[3.1.0]hexan- [(1S)-1-hydroxyethyl]pyridin-3-yl)methyl]-1H- 3-yl}pyridin-3-yl)methyl]-1H-pyrazole-4- pyrazole-4-carboxylate carboxylate

Intermediate 54

(5-{3-Azabicyclo[3.1.0]hexan-3-yl}-3-chloropyrazin-2-yl)methanol

[0470] ##STR00502##

[0471] LiBH.sub.4 (744 mg) is added portionwise to an ice-cooled mixture of methyl 5-{3-azabicyclo[3.1.0]hexan-3-yl}-3-chloropyrazine-2-carboxylate (4.33 g) in THF (80 mL). The mixture is stirred for 2 h at rt. After cooling to 0° C. aqueous HCl (4 M, 10 mL) is added and the mixture is stirred for 10 minutes. Then the mixture is partitioned between saturated aqueous NaHCO.sub.3 and EtOAc. The aqueous phase is extracted twice with EtOAc. The combined organic phases are dried (MgSO.sub.4), concentrated in vacuo and the residue is chromatographed on silica gel (petroleum ether/EtOAc 80:20.fwdarw.50:50) to give the title compound. LC (Method 2): t.sub.R=0.85 min; Mass spectrum (ESI+): m/z=226 [M+H].sup.+.

Intermediate 55

Ethyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3-yl)methyl]-1H-pyrazole-4-carboxylate

[0472] ##STR00503##

[0473] Under argon atmosphere an ice-cooled mixture of (6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3-yl)methanol (20 g) and DIPEA (32 mL) in DCM (400 mL) is treated dropwise with CH.sub.3SO.sub.2Cl (7.2 mL). The mixture is stirred for 15 minutes and then treated with ethyl 1H-pyrazole-4-carboxylate (12 g). After stirring for 4 h at rt the mixture is partitioned between water and DCM. The organic phase is dried (MgSO.sub.4) and concentrated in vacuo to give the crude product which is directly used in the next step.

[0474] LC (Method 2): t.sub.R=0.76 min; Mass spectrum (ESI+): m/z=363 [M+H].sup.+.

[0475] Intermediate 55-1 is prepared in analogy to Intermediate 55:

TABLE-US-00099 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M + H].sup.+ LC Method 55-1 [00504] embedded image 1.02 327 Method 1

TABLE-US-00100 Intermediate Reaction comment 55-1 After the addition of ethyl 1H-pyrazole-4-carboxylate the reaction mixture is stirred for 12 h at rt.

TABLE-US-00101 Name of Name of Intermediate Name Starting Material 1 Starting Material 2 55-1 Ethyl 1-[(6-{3-azabicyclo[3.1.0]- (6-{3-Azabicyclo[3.1.0]hexan- Ethyl 1H-pyrazole-4- hexan-3-yl}-4-methylpyridin-3- 3-yl}-4-methylpyridin-3-yl)- carboxylate yl)-methyl]-1H-pyrazole-4- methanol carboxylate

Intermediate 56

1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(methoxymethyl)pyridin-3-yl)methyl]-1H-1,2,3-triazole-4-carboxylic acid

[0476] ##STR00505##

[0477] To a solution of ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-(hydroxymethyl)pyridin-3-yl)methyl]-1H-1,2,3-triazole-4-carboxylate (86 mg) in DMF (2 mL) is added at 0° C. NaH (60% in mineral oil, 25 mg). The mixture is stirred for 30 minutes at rt, treated with CH.sub.3I (16 μL) and stirred for 12 h. Water is carefully added. The mixture is concentrated in vacuo and the residue is taken up in DCM/isopropanol 1:1. Then the mixture is filtered and the filtrate is concentrated in vacuo to give the crude product which is directly used in the next step.

[0478] LC (Method 2): t.sub.R=0.58 min; Mass spectrum (ESI+): m/z=330 [M+H].sup.+.

[0479] Intermediates 56-1 to 56-2 are prepared in analogy to Intermediate 56:

TABLE-US-00102 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M + H].sup.+ LC Method 56-1 [00506] embedded image 0.71 379 Method 2 56-2 [00507] 0.53 329 Method 2

TABLE-US-00103 Intermediate Reaction comment 56-1 Iodoethane is used instead of CH.sub.3I. 56-2 After addition of CH.sub.3I the mixture is stirred for 4 h. Then aqueous NaOH (4M) is added until pH of 12 is reached and the mixture is stirred for 1 h at 50° C. The mixture is neutralized by addition of acetic acid and purified by HPLC on reversed phase (ACN, water).

TABLE-US-00104 Intermediate Name Name of Starting Material 56-1 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- Ethyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- yl}-2-(ethoxymethyl)pyridin-3-yl)methyl]-1H- hexan-3-yl}-2-(hydroxymethyl)pyridin-3- pyrazole-4-carboxylic acid yl)methyl]-1H-pyrazole-4-carboxylate 56-2 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 1-[(6-{3-azabicydo[3.1.0]hexan-3-yl}-2- (methoxymethyl)pyridin-3-yl)methyl]-1H- (hydroxymethyl)pyridin-3-yl)methyl]-1H- imidazole-4-carboxylic acid imidazole-4-carboxylate

Intermediate 57

1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-methoxypyridin-3-yl)methyl]-1H-imidazole-4-carboxylic acid

[0480] ##STR00508##

[0481] A mixture of ethyl 1-[(2-bromo-6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}pyridin-3-yl)methyl]-1H-imidazole-4-carboxylate (190 mg) and NaOCH.sub.3 (1 M in MeOH, 4.5 mL) is heated for 2.5 h to 155° C. and for 3 h to 165° C. Purification by HPLC on reversed phase (ACN, water) gives the title compound. LC (Method 2): t.sub.R=0.84 min.

[0482] Intermediate 57-1 is prepared in analogy to Intermediate 57:

TABLE-US-00105 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M + H].sup.+ LC Method 57-1 [00509] embedded image 0.85 Method 2

TABLE-US-00106 Intermediate Reaction comment 57-1 NaOCH.sub.2CH.sub.3 in EtOH is used instead of NaOCH.sub.3 in MeOH. The reaction is conducted for 12 h at 150° C. Intermediate Name Name of Starting Material 57-1 1-[(6-{6,6-Difluoro-3-azabicydo[3.1.0]hexan-3- Ethyl 1-[(2-chloro-6-{6,6-difluoro-3- yl}-2-ethoxypyridin-3-yl)methyl]-1H-imidazole- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- 4-carboxylic acid yl)methyl]-1H-imidazole-4-carboxylate

Intermediate 58

1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(hydroxymethyl)pyridin-3-yl)methyl]-1H-imidazole-4-carboxylic acid

Intermediate 58-1

Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-(hydroxymethyl)pyridin-3-yl)methyl]-1H-imidazole-4-carboxylate

[0483] ##STR00510##

[0484] LiBH.sub.4 (356 mg) is added portionwise to a mixture of ethyl 6-{3-azabicyclo[3.1.0]hexan-3-yl}-3-{[4-(ethoxycarbonyl)-1H-imidazol-1-yl]methyl}pyridine-2-carboxylate (1.65 g) in THF (25 mL). The mixture is stirred for 14 h at rt, cooled to 0° C. and treated with aqueous HCl (1 M, 5 mL). Then the mixture is neutralized by addition of NaOH (4 M) and purified by HPLC on reversed phase (ACN, water) to give the title compounds. [0485] 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(hydroxymethyl)pyridin-3-yl)methyl]-1H-imidazole-4-carboxylic acid:

[0486] LC (Method 1): t.sub.R=0.64 min; Mass spectrum (ESI+): m/z=315 [M+H].sup.+. [0487] Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-(hydroxymethyl)pyridin-3-yl)methyl]-1H-imidazole-4-carboxylate:

[0488] LC (Method 1): t.sub.R=0.94 min; Mass spectrum (ESI+): m/z=343 [M+H].sup.+.

Intermediate 59

2-Hydroxy-5-iodo-4-methylpyridine-3-carbonitrile

[0489] ##STR00511##

[0490] Under argon atmosphere an ice-cooled mixture of 2-hydroxy-4-methylpyridine-3-carbonitrile (3 g) in DCM (100 mL) is treated with trifluoroacetic acid (5 mL). Then N-iodosuccinimide (7.55 g) is added portionwise. The mixture is stirred for 3 h while warming to rt. The mixture is concentrated in vacuo, half-saturated aqueous Na.sub.2S.sub.2O.sub.3 is added and the mixture is stirred for 10 minutes. The precipitate is collected by filtration, washed with water and diethylether and dried in vacuo to give the title compound. LC (Method 2): t.sub.R=0.72 min; Mass spectrum (ESI+): m/z=261 [M+H].sup.+.

Intermediate 60

2-Chloro-5-iodo-4-methylpyridine-3-carbonitrile

[0491] ##STR00512##

[0492] A mixture of 2-hydroxy-5-iodo-4-methylpyridine-3-carbonitrile (6.27 g) in POCl.sub.3 is stirred for 5 h at 100° C. The mixture is concentrated in vacuo. The residue is taken up in DCM (200 mL) and treated with water. Then the mixture is neutralized by careful addition of saturated aqueous NaHCO.sub.3. The phases are separated and the aqueous phase is extracted twice with DCM. The combined organic phases are washed with saturated aqueous NaHCO.sub.3, dried (MgSO.sub.4) and concentrated in vacuo to give the crude product which is directly used in the next step.

[0493] LC (Method 1): t.sub.R=1.02 min; Mass spectrum (ESI+): m/z=278 [M+H].sup.+.

Intermediate 61

2-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-5-formyl-4-methylpyridine-3-carbonitrile

[0494] ##STR00513##

[0495] A mixture of 2-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-5-iodo-4-methylpyridine-3-carbonitrile (2.3 g) in THF (30 mL) is cooled to −78° C., treated dropwise with isopropylmagnesium chloride (iPrMgCl, 2 M solution in THF, 3.82 mL) and stirred for 30 minutes. Then the mixture is warmed to 0° C., treated dropwise with DMF (2.47 mL) and stirred for 40 minutes. Water is carefully added and the mixture is partitioned between half-saturated aqueous NH.sub.4Cl and EtOAc. The aqueous phase is extracted with EtOAc and the combined organic phases are concentrated in vacuo to give the crude product which is directly used in the next step.

[0496] LC (Method 2): t.sub.R=0.96 min; Mass spectrum (ESI+): m/z=264 [M+H].sup.+.

[0497] Intermediate 61-1 is prepared in analogy to Intermediate 61:

TABLE-US-00107 Mass spectrum (ESI+): Inter- m/z LC mediate Structure t.sub.R [M + H].sup.+ Method 61-1 [00514] embedded image 0.98 228 Method 2

TABLE-US-00108 Intermediate Name Name of Starting Material 61-1 2-{3-Azabicyclo[3.1.0]hexan-3-yl}-5-formyl-4- 2-{3-Azabicyclo[3.1.0]hexan-3-yl}-5-iodo-4- methylpyridine-3-carbonitrile methylpyridine-3-carbonitrile

Intermediate 62

N′-[(E)-(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3-yl)methylidene]acetohydrazide

[0498] ##STR00515##

[0499] A mixture of 6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridine-3-carbaldehyde (5.8 g) and acetohydrazide (2.36 g) in MeOH (100 mL) is refluxed for 12 h. The mixture is concentrated in vacuo, taken up in toluene, treated with p-toluenesulfonic acid (100 mg) and refluxed in a Dean-Stark apparatus for 16 h. After cooling to rt the precipitate is collected by filtration, washed with tert.-butyl-methyl-ether and dried in vacuo to give the title compound. LC (Method 2): t.sub.R=0.64 min; Mass spectrum (ESI+): m/z=295 [M+H].sup.+.

[0500] Intermediate 62-1 is prepared in analogy to Intermediate 62:

TABLE-US-00109 Mass spectrum (ESI+): Inter- m/z LC mediate Structure t.sub.R [M + H].sup.+ Method 62-1 [00516] embedded image 0.61 259 Method 2

TABLE-US-00110 Intermediate Name Name of Starting Material 62-1 N′-[(E)-(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- 6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- methylpyridin-3-yl)methylidene]acetohydrazide methylpyridine-3-carbaldehyde

Intermediate 63

N′-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3-yl)methyl]acetohydrazide

[0501] ##STR00517##

[0502] A mixture of N′-[(E)-(6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3-yl)methylidene]acetohydrazide (5.6 g) and 10% palladium on carbon (300 mg) in MeOH (80 mL) and THF (20 mL) is shaken under hydrogen atmosphere (3 bar) at rt for 3.5 h. The mixture is filtered, the filtrate is concentrated and the residue is taken up in tert.-butyl-methyl-ether (100 mL) and EtOAc (10 mL). After stirring for 3 h the precipitate is collected by filtration, washed with tert.-butyl-methyl-ether and dried in vacuo to give the title compound.

[0503] LC (Method 2): t.sub.R=0.58 min; Mass spectrum (ESI+): m/z=297 [M+H].sup.+.

[0504] Intermediate 63-1 is prepared in analogy to Intermediate 63:

TABLE-US-00111 Mass spectrum (ESI+): Inter- m/z LC mediate Structure t.sub.R [M + H].sup.+ Method 63-1 [00518] embedded image 0.57 261 Method 2

TABLE-US-00112 Intermediate Name Name of Starting Material 63-1 N′-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- N′-[(E)-(6-{3-Azabicydo[3.1.0]hexan-3-yl}-2- methylpyridin-3-yl)methyl]acetohydrazide methylpyridin-3-yl)methylidene]acetohydrazide

Intermediate 64

Ethyl 3-(chloromethyl)-1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3-yl)methyl]-1H-pyrazole-4-carboxylate

[0505] ##STR00519##

[0506] A mixture of N′-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3-yl)methyl]acetohydrazide (1.9 g) and ethyl 4-chloro-2-(ethoxymethylidene)-3-oxobutanoate (1.68 g) in EtOH (30 mL) is stirred for 12 h at rt. The mixture is concentrated and then partitioned between DCM and saturated aqueous Na.sub.2CO.sub.3. The aqueous phase is extracted with DCM. The combined organic phases are washed with brine, dried (MgSO.sub.4) and concentrated in vacuo to give the title compound. LC (Method 2): t.sub.R=0.82 min; Mass spectrum (ESI+): m/z=411 [M+H].sup.+.

[0507] Intermediate 64-1 is prepared in analogy to Intermediate 64:

TABLE-US-00113 Mass spectrum (ESI+): Inter- m/z LC mediate Structure t.sub.R [M + H].sup.+ Method 64-1 [00520] embedded image 0.81 375 Method 2

TABLE-US-00114 Intermediate Name Name of Starting Material 64-1 Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- N′-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- methylpyridin-3-yl)methyl]-3-(chloromethyl)- methylpyridin-3-yl)methyl]acetohydrazide 1H-pyrazole-4-carboxylate

Intermediate 65

Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3-yl)methyl]-3-(cyanomethyl)-1H-pyrazole-4-carboxylate

[0508] ##STR00521##

[0509] A mixture of ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3-yl)methyl]-3-(chloromethyl)-1H-pyrazole-4-carboxylate (700 mg) and KCN (250 mg) in DMSO (5 mL) and water (2 mL) is heated to 85° C. for 2 h. The mixture is partitioned between water and EtOAc. The aqueous phase is extracted 4 times with EtOAc. The combined organic phases are washed with brine, dried (MgSO.sub.4) and concentrated in vacuo to give the title compound.

[0510] LC (Method 2): t.sub.R=0.74 min; Mass spectrum (ESI+): m/z=366 [M+H].sup.+.

Intermediate 66

1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3-yl)methyl]-3-(cyanomethyl)-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide

[0511] ##STR00522##

[0512] A mixture of 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3-yl)methyl]-3-(cyanomethyl)-1H-pyrazole-4-carboxylic acid (230 mg), DIPEA (466 μL) and O-(7-azabenzotriazol-1-yl)-N,N,N′,N′-tetramethyluronium-hexafluorophosphat (HATU, 272 mg) in DMF (4 mL) is stirred for 5 min. (4R)-1-Methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-amine dihydrochloride (143 mg) is added and the mixture is stirred for 2 h. The mixture is partitioned between water and EtOAc. The aqueous phase is extracted three times with EtOAc. The combined organic phases are washed with brine, dried (MgSO.sub.4), concentrated in vacuo and chromatographed on silica gel (DCM/(DCM/MeOH/7 N NH.sub.3 in MeOH 50:48:2) 90:10.fwdarw.60:40) to give the title compound.

[0513] LC (Method 2): t.sub.R=0.63 min; Mass spectrum (ESI.sup.+): m/z=457 [M+H].sup.+.

[0514] Intermediates 66-1 to 66-4 are prepared in analogy to Intermediate 66:

TABLE-US-00115 Mass spectrum (ESI+): Inter- m/z LC mediate Structure t.sub.R [M + H].sup.+ Method 66-1 [00523] embedded image 0.97 496 Method 1 66-2 [00524] 0.64 372 Method 2 66-3 [00525] 0.78 578 Method 2 [00526] (mixture of isomers) 66-4 [00527] 0.76 564 Method 2 [00528] (mixture of isomers)

TABLE-US-00116 Intermediate Reaction comment 66-3 The product is obtained as a mixture of isomers. 66-4 The product is obtained as a mixture of isomers.

TABLE-US-00117 Intermediate Name Name of Starting Material 66-1 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- methylpyridin-3-yl)methyl]-3-bromo-N-[(4R)-1- methylpyridin-3-yl)methyl]-3-bromo-1H- methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4- pyrazole-4-carboxylic acid yl]-1H-pyrazole-4-carboxamide 66-2 1-[(2-Chloro-4-methylpyrimidin-5-yl)methyl]-N- 1-[(2-Chloro-4-methylpyrimidin-5-yl)methyl]- [(4R)-1-methyl-1H,4H,5H,6H-cydopenta[d]- 1H-pyrazole-4-carboxylic acid imidazol-4-yl]-1H-pyrazole-4-carboxamide 66-3 3-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- 3-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- (methoxymethyl)pyridin-3-yl)methyl]-N-[(4R)-1- (methoxymethyl)pyridin-3-yl)methyl]-1-{[2- methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4- (trimethylsilyl)ethoxy]methyl}-1H-pyrazole-5- yl]-1-{[2-(trimethylsilyl)ethoxy]methyl}-1H- carboxylic acid pyrazole-5-carboxamide and and 5-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- 5-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- (methoxymethyl)pyridin-3-yl)methyl]-1-{[2- (methoxymethyl)pyridin-3-yl)methyl]-N-[(4R)-1- (trimethylsilyl)ethoxy]methyl}-1H-pyrazole-3- methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4- carboxylic acid yl]-1-{[2-(trimethylsilyl)ethoxy]methyl}-1H- (mixture of isomers) pyrazole-3-carboxamide (mixture of isomers) 66-4 3-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- 3-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- (hydroxymethyl)pyridin-3-yl)methyl]-N-[(4R)-1- (hydroxymethyl)pyridin-3-yl)methyl]-1-{[2- methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4- (trimethylsilyl)ethoxy]methyl}-1H-pyrazole-5- yl]-1-{[2-(trimethylsilyl)ethoxy]methyl}-1H- carboxylic acid pyrazole-5-carboxamide and and 5-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- 5-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- (hydroxymethyl)pyridin-3-yl)methyl]-1-{[2- (hydroxymethyl)pyridin-3-yl)methyl]-N-[(4R)-1- (trimethylsilyl)ethoxy]methyl}-1H-pyrazole-3- methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4- carboxylic acid yl]-1-{[2-(trimethylsilyl)ethoxy]methyl}-1H- (mixture of isomers) pyrazole-3-carboxamide (mixture of isomers)

Intermediate 67

Methyl 2-{1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3-yl)methyl]-4-{[(4R)-1-methyl-1H,4H,5H,6H- cyclopenta[d]imidazol-4-yl]carbamoyl}-1H-pyrazol-3-yl}acetate

[0515] ##STR00529##

[0516] A mixture of 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3-yl)methyl]-3-(cyanomethyl)-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide (330 mg) in CH.sub.3COOH (2 mL) and concentrated aqueous HCl (2 mL) is heated for 1 h to 110° C. The mixture is cooled to rt treated with aqueous NaOH (4 M, 15 mL) and stirred for 10 minutes. Then aqueous HCl (4 M, 15 mL) is added and the mixture is washed twice with EtOAc. The aqueous phase is concentrated in vacuo and the residue is added to a mixture of acetyl chloride (50 μL) in MeOH (10 mL). The mixture is heated to 90° C. for 90 minutes. After cooling to rt the mixture is neutralized with aqueous NaOH (1 M), concentrated in vacuo and the residue is chromatographed on silica gel (cyclohexane/EtOAc 90:10.fwdarw.70:30) to give the title compound.

[0517] LC (Method 2): t.sub.R=0.63 min; Mass spectrum (ESI.sup.+): m/z=490 [M+H].sup.+.

Intermediate 68

Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-fluoropyridin-3-yl)methyl]-1H-imidazole-4-carboxylate

[0518] ##STR00530##

[0519] A mixture of ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-chloropyridin-3-yl)methyl]-1H-imidazole-4-carboxylate (200 mg) and tetrabutylammonium fluoride trihydrate (269 mg) in DMF (2 mL) is heated to 80° C. for 12 h. After cooling to rt the mixture is partitioned between saturated aqueous NaHCO.sub.3 and EtOAc. The aqueous phase is extracted for 4 times with EtOAc. The combined organic phases are concentrated in vacuo and purified by HPLC on reversed phase (AON, water) to give the title compound. LC (Method 1): t.sub.R=0.96 min.

Intermediate 69

2-{3-Azabicyclo[3.1.0]hexan-3-yl}-5-bromopyridine-3-carbonitrile

[0520] ##STR00531##

[0521] Under argon atmosphere a mixture of 5-bromo-2-chloropyridine-3-carbonitrile (1.0 g), 3-azabicyclo[3.1.0]hexane hydrochloride (605 mg) and DIPEA (2 mL) in DMF (10 mL) is heated to 80° C. for 2 h. The mixture is cooled, concentrated, partitioned between water and EtOAc and the phases are separated. The aqueous is extracted twice with EtOAc. The combined organic phases are washed with brine, dried (MgSO.sub.4), concentrated and the residue is chromatographed on silica gel (petroleum ether/EtOAc 98:2.fwdarw.95:5) to give the title compound.

[0522] LC (Method 2): t.sub.R=1.13 min; Mass spectrum (ESI+): m/z=264 [M+H].sup.+.

[0523] Intermediate 69-1 is prepared in analogy to Intermediate 69:

TABLE-US-00118 Mass spectrum (ESI+): LC Intermediate Structure t.sub.R m/z [M + H].sup.+ Method 69-1 [00532] embedded image 1.25 311 Method 1

TABLE-US-00119 Intermediate Reaction comment 69-1 The reaction is conducted for 12 h at 80° C.

TABLE-US-00120 Intermediate Name Name of Starting Material 1 Name of Starting Material 2 69-1 Methyl 6-{3-azabicyclo[3.1.0]- Methyl 5-bromo-6-chloro-2- 3-azabicydo[3.1.0]hexane hexan-3-yl}-5-bromo-2- methylpyridine-3-carboxylate hydrochloride methylpyridine-3-carboxylate

Intermediate 70

Methyl 6-{3-azabicyclo[3.1.0]hexan-3-yl}-5-cyanopyridine-3-carboxylate

[0524] ##STR00533##

[0525] Under argon atmosphere a mixture of 2-{3-azabicyclo[3.1.0]hexan-3-yl}-5-bromopyridine-3-carbonitrile (773 mg) and triethylamine (489 μL) and (1,1′-bis(diphenylphosphino)ferrocene)palladium(II) dichloride (119 mg) in DMF (15 mL) and MeOH (15 mL) is heated to 80° C. for 22 h under a CO atmosphere of 10 bar. The mixture is cooled, concentrated in vacuo and the residue is purified by HPLC on reversed phase (ACN, water) to give the title compound.

[0526] LC (Method 1): t.sub.R=0.99 min; Mass spectrum (ESI+): m/z=244 [M+H].sup.+.

[0527] Intermediates 70-1 to 70-3 are prepared in analogy to Intermediate 70:

TABLE-US-00121 Mass spectrum (ESI+): Inter- m/z LC mediate Structure t.sub.R [M + H].sup.+ Method 70-1 [00534] embedded image 0.64 476 Method 2 70-2 [00535] 1.14 437 Method 2 70-3 [00536] 0.72 366 Method 2

TABLE-US-00122 Intermediate Reaction comment 70-1 MeOH is used instead of a DMF/MeOH mixture. The reaction is conducted at 100° C. for 2 h under a CO atmosphere of 12 bar 70-2 EtOH is used instead of MeOH. Bis(triphenylphosphine)palladium(II) dichloride is used instead of (1,1′-bis(diphenylphosphino)ferrocene)palladium(II) dichloride. The reaction is conducted at 90° C. for 17 h under a CO atmosphere of 5 bar. 70-3 The reaction is conducted at 90° C. for 6 h.

TABLE-US-00123 Intermediate Name Name of Starting Material 70-1 Methyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- methylpyridin-3-yl)methyl]-4-{[(4R)-1-methyl- methylpyridin-3-yl)methyl]-3-bromo-N-[(4R)-1- 1H,4H,5H,6H-cyclopenta[d]imidazol-4- methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4- yl]carbamoyl}-1H-pyrazole-3-carboxylate yl]-1H-pyrazole-4-carboxamide 70-2 Ethyl 6-{6,6-difluoro-3-azabicydo[3.1.0]hexan- Ethyl 5-[(2-bromo-6-{6,6-difluoro-3- 3-yl}-3-{[5-(ethoxycarbonyl)thiophen-2- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- yl]methyl}pyridine-2-carboxylate yl)methyl]thiophene-2-carboxylate 70-3 Methyl 2-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- 3-{5-[(5-Bromo-1,3-thiazol-2-yl)methyl]-6- hexan-3-yl}-2-methylpyridin-3-yl)methyl]-1,3- methylpyridin-2-yl}-6,6-difluoro-3- thiazole-5-carboxylate azabicyclo[3.1.0]hexane

Intermediate 71

2-{3-Azabicyclo[3.1.0]hexan-3-yl}-5-(hydroxymethyl)pyridine-3-carbonitrile

[0528] ##STR00537##

[0529] A mixture of methyl 6-{3-azabicyclo[3.1.0]hexan-3-yl}-5-cyanopyridine-3-carboxylate (300 mg) in THF (5 mL) is cooled to −50° C. and treated dropwise with LiAlH.sub.4 (1 M solution in THF, 1.4 mL). The mixture is stirred for 3 h at −20° C. and then carefully treated with water (1 mL). After dilution with DCM the mixture is stirred for 30 minutes. The precipitate is filtered off and the filter cake is washed with DCM. The combined filtrates are diluted with water. The phases are separated. The aqueous phase is extracted twice with DCM. The combined organic phases are dried (MgSO.sub.4), concentrated and purified by HPLC on reversed phase (ACN, water) to give the title compound.

[0530] LC (Method 1): t.sub.R=0.81 min; Mass spectrum (ESI+): m/z=216 [M+H].sup.+.

[0531] Intermediates 71-1 to 71-2 are prepared in analogy to Intermediate 71:

TABLE-US-00124 Mass spectrum (ESI+): Inter- m/z LC mediate Structure t.sub.R [M + H].sup.+ Method 71-1 [00538] embedded image 0.93 259 Method 1 71-2 [00539] 0.77 283 Method 2

TABLE-US-00125 Intermediate Reaction comment 71-1 The reaction is conducted for 3 h at −25° C. Then 10% NH.sub.4Cl in water is carefully added. The mixture is partitioned between water and EtOAc and filtered over celite. The phases are separated and the aqueous phase is extracted with EtOAc. The combined organic phases are dried (MgSO.sub.4) and concentrated in vacuo to give the crude product, which is used directly in the next step.

TABLE-US-00126 Intermediate Name Name of Starting Material 71-1 (6-{3-Azabicyclo[3.1.0]hexan-3-yl}-4- Methyl 6-{3-azabicyclo[3.1.0]hexan-3-yl}-4- (trifluoromethyl)pyridin-3-yl)methanol (trifluoromethyl)pyridine-3-carboxylate 71-2 (6-{3-Azabicyclo[3.1.0]hexan-3-yl}-5-bromo-2- Methyl 6-{3-azabicydo[3.1.0]hexan-3-yl}-5- methylpyridin-3-yl)methanol bromo-2-methylpyridine-3-carboxylate

Intermediate 72

Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3-yl)methyl]-3-(methoxymethyl)-1H-pyrazole-4-carboxylate

[0532] ##STR00540##

[0533] A mixture of ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3-yl)methyl]-3-(chloromethyl)-1H-pyrazole-4-carboxylate (100 mg) and NaI (10 mg) in MeOH (4 mL) is heated under argon atmosphere in a microwave vial at 90° C. for 12 h. The mixture is diluted with MeOH and water and purified by HPLC on reversed phase (ACN, water) to give the title compound. LC (Method 1): t.sub.R=1.07 min; Mass spectrum (ESI+): m/z=371 [M+H].sup.+.

[0534] Intermediate 72-1 is prepared in analogy to Intermediate 72:

TABLE-US-00127 Mass spectrum (ESI+): Inter- m/z LC mediate Structure t.sub.R [M + H].sup.+ Method 72-1 [00541] embedded image 1.04 407 Method 1

TABLE-US-00128 Intermediate Reaction comment 72-1 The reaction is conducted at 100° C.

TABLE-US-00129 Intermediate Name Name of Starting Material 72-1 Ethyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- Ethyl 3-(chloromethyl)-1-[(6-{6,6-difluoro-3- hexan-3-yl}-2-methylpyridin-3-yl)methyl]-3- azabicydo[3.1.0]hexan-3-yl}-2-methylpyridin-3- (methoxymethyl)-1H-pyrazole-4-carboxylate yl)methyl]-1H-pyrazole-4-carboxylate

Intermediate 73

Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-5-cyano-2-methylpyridin-3-yl)methyl]-1H-imidazole-4-carboxylate

[0535] ##STR00542##

[0536] In a microwave vial N,N,N′,N′-tetramethylethylenediamine (196 μL) is added to a mixture of ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-5-bromo-2-methylpyridin-3-yl)methyl]-1H-imidazole-4-carboxylate (530 mg), NaCN (77 mg), CuCN (25 mg) and Kl (43 mg) in toluene (15 mL). The vial is sealed and the mixture is heated to 130° C. for 18 h. After cooling to rt the mixture is partitioned between water and EtOAc. The aqueous phase is extracted with EtOAc. The combined organic phases are dried (MgSO.sub.4), concentrated and the residue is purified by HPLC on reversed phase (ACN, water) to give the title compound.

[0537] LC (Method 1): t.sub.R=0.99 min; Mass spectrum (ESI+): m/z=352 [M+H].sup.+.

[0538] Intermediate 73-1 is prepared in analogy to Intermediate 73:

TABLE-US-00130 Mass spectrum (ESI+): Inter- m/z LC mediate Structure t.sub.R [M + H].sup.+ Method 73-1 [00543] embedded image 1.04 338 Method 1

TABLE-US-00131 Intermediate Name Name of Starting Material 73-1 Methyl 1-[(6-{3-azabicydo[3.1.0]hexan-3-yl}-5- Methyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-5- cyano-2-methylpyridin-3-yl)methyl]-1H- bromo-2-methylpyridin-3-yl)methyl]-1H- pyrazole-4-carboxylate pyrazole-4-carboxylate

Intermediate 74

1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3-yl)methyl]-3-(2-hydroxypropan-2-yl)-1H-pyrazole-4-carboxylic acid hydrochloride

[0539] ##STR00544##

[0540] A mixture of ethyl 1-[(6-{3-azabicycio[3.1.0]hexan-3-yl}-2-methylpyridin-3-yl)methyl]-3-(prop-1-en-2-yl)-1H-pyrazole-4-carboxylate (66 mg) in aqueous HCl (4 M, 5 mL) is heated to 60° C. for 12 h. The mixture is concentrated in vacuo to give the crude product, which is directly used in the next step.

[0541] LC (Method 2): t.sub.R=0.67 min; Mass spectrum (ESI+): m/z=357 [M+H].sup.+.

Intermediate 75

Methyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-5-bromo-2-methylpyridin-3-yl)methyl]-1H-pyrazole-4-carboxylate

[0542] ##STR00545##

[0543] A mixture of 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-5-bromo-2-methylpyridin-3-yl)methyl]-1H-pyrazole-4-carboxylic acid (920 mg) and concentrated aqueous H.sub.2SO.sub.4 (182 μL) in MeOH (10 mL) is heated to 40° C. for 12 h. After cooling to rt the mixture is partitioned between EtOAc and saturated aqueous NaHCO.sub.3. The aqueous phase is extracted with EtOAc. The combined organic phases are dried (MgSO.sub.4), concentrated and the residue is purified by HPLC on reversed phase (ACN, water) to give the title compound.

[0544] LC (Method 1): t.sub.R=1.17 min; Mass spectrum (ESI+): m/z=391 [M+H].sup.+.

[0545] Intermediate 75-1 is prepared in analogy to Intermediate 75:

TABLE-US-00132 Mass spectrum (ESI+): Inter- m/z LC mediate Structure t.sub.R [M + H].sup.+ Method 75-1 [00546] embedded image 1.07 Method 1

TABLE-US-00133 Intermediate Reaction comment 75-1 The reaction is conducted for 12 h at 70° C.

TABLE-US-00134 Intermediate Name Name of Starting Material 75-1 Methyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-5- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-5- bromo-2-methylpyridin-3-yl)methyl]-1H- bromo-2-methylpyridin-3-yl)methyl]-1H- imidazole-4-carboxylate imidazole-4-carboxylic acid

Intermediate 76

Methyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2,5-dimethylpyridin-3-yl)methyl]-1H-imidazole-4-carboxylate

[0546] ##STR00547##

[0547] In a microwave vial a mixture of methyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-5-bromo-2-methylpyridin-3-yl)methyl]-1H-imidazole-4-carboxylate (120 mg), trimethylboroxine (77 mg) and K.sub.2CO.sub.3 (127 mg) in DMF (4 mL) is purged for minutes with argon. Tetrakis(triphenylphosphine)palladium(0) (Pd(PPh.sub.3).sub.4, 71 mg) is added, the vial is sealed and the mixture is heated for 12 h to 110° C. After cooling to rt the mixture is partitioned between half-saturated aqueous NaCl and EtOAc. The aqueous phase is extracted with EtOAc. The combined organic phases are dried (MgSO.sub.4), concentrated and the residue is purified by HPLC on reversed phase (ACN, water) to give the title compound.

[0548] LC (Method 2): t.sub.R=0.63 min; Mass spectrum (ESI+): m/z=327 [M+H].sup.+.

[0549] Intermediate 76-1 is prepared in analogy to Intermediate 76:

TABLE-US-00135 Mass spectrum (ESI+): Inter- m/z LC mediate Structure t.sub.R [M + H].sup.+ Method 76-1 [00548] embedded image 1.10 327 Method 1

TABLE-US-00136 Intermediate Name Name of Starting Material 76-1 Methyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}- Methyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-5- 2,5-dimethylpyridin-3-yl)methyl]-1H-pyrazole- bromo-2-methylpyridin-3-yl)methyl]-1H- 4-carboxylate pyrazole-4-carboxylate

Intermediate 77

Ethyl 1-[(6-chloro-2-cyanopyridin-3-yl)methyl]-1H-pyrazole-4-carboxylate

[0550] ##STR00549##

[0551] A mixture of 6-chloro-3-(chloromethyl)pyridine-2-carbonitrile (37 mg), ethyl 1H-pyrazole-4-carboxylate (30 mg) and Cs.sub.2CO.sub.3 (100 mg) in THF (2 mL) is stirred for 8 h at rt. Then the mixture is neutralized by addition of trifluoroacetic acid and purified by HPLC on reversed phase (ACN, water) to give the title compound.

[0552] LC (Method 1): t.sub.R=0.94 min; Mass spectrum (ESI+): m/z=291 [M+H].sup.+.

Intermediate 78

Ethyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-cyanopyridin-3-yl)methyl]-1H-pyrazole-4-carboxylate

[0553] ##STR00550##

[0554] A mixture of ethyl 1-[(6-chloro-2-cyanopyridin-3-yl)methyl]-1H-pyrazole-4-carboxylate (1 g), 3-azabicyclo[3.1.0]-hexane hydrochloride (411 mg) and DIPEA (1.8 mL) in NMP (20 mL) is stirred for 12 h at 140° C. After cooling to rt the mixture is partitioned between water and EtOAc. The aqueous phase is extracted with EtOAc. The combined organic phases are washed with water, dried (MgSO.sub.4), concentrated and the residue is purified by HPLC on reversed phase (ACN, water) to give the title compound. LC (Method 1): t.sub.R=1.04 min; Mass spectrum (ESI+): m/z=338 [M+H].sup.+.

Intermediate 79

2-Chloro-4-methylpyrimidine-5-carboxylic acid

[0555] ##STR00551##

[0556] A mixture of ethyl 2-chloro-4-methylpyrimidine-5-carboxylate (25 g) and NaOH (6.5 g) in water (200 mL) is stirred at 40° C. for 3 h. After cooling to rt the mixture is treated with aqueous HCl (4 M) until a pH-value of 2 is reached. The precipitate is collected by filtration, washed with water and dried in vacuo to give the title compound.

[0557] LC (Method 2): t.sub.R=0.68 min; Mass spectrum (ESI+): m/z=173 [M+H].sup.+.

Intermediate 80

(2-Chloro-4-methylpyrimidin-5-yl)methanol

[0558] ##STR00552##

[0559] A mixture of 2-chloro-4-methylpyrimidine-5-carboxylic acid (8.5 g) and N-methylmorpholine (5.14 mL) in 1,2-dimethoxyethane (200 mL) is cooled to −10° C. and treated dropwise with isobutylchloroformate (6.2 mL). The mixture is stirred for 30 minutes and then treated dropwise with a solution of NaBH.sub.4 (1.81 g) in water (20 mL). The mixture is stirred for 30 minutes while warming to rt and then partitioned between water and EtOAc. The aqueous phase is extracted with EtOAc for 3 times. The combined organic phases are washed with brine, dried (MgSO.sub.4), concentrated and the residue is chromatographed on silica gel (petroleum ether/(EtOAc/MeOH 8:2) 70:30) to give the title compound. LC (Method 2): t.sub.R=0.51 min; Mass spectrum (ESI+): m/z=159 [M+H].sup.+.

Intermediate 81

(2-{3-Azabicyclo[3.1.0]hexan-3-yl}-4-methylpyrimidin-5-yl)methanol

[0560] ##STR00553##

[0561] Diisobutylaluminiumhydride (1 M in THF, 80 mL) is added dropwise at −10° C. to a mixture of ethyl 2-{3-azabicyclo[3.1.0]hexan-3-yl}-4-methylpyrimidine-5-carboxylate (8.7 g) in THF (70 mL). The mixture is stirred for 1 h while warming to 0° C. This mixture is then added dropwise under ice-cooling to a mixture of aqueous NaOH (4 M, 6 mL) in water (150 mL). After stirring for 1 h the mixture is filtered over celite. The filter cake is washed with EtOAc/MeOH 9:1. The combined filtrates are dried (MgSO.sub.4), concentrated and the residue is chromatographed on silica gel (EtOAc/MeOH 95:5.fwdarw.95:5) to give the title compound.

[0562] LC (Method 2): t.sub.R=0.59 min; Mass spectrum (ESI+): m/z=206 [M+H].sup.+.

Intermediate 82

Methyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}pyridin-3-yl)methyl]-1H-pyrrole-3-carboxylate and

Intermediate 83

Methyl 5-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}pyridin-3-yl)methyl]-1H-pyrrole-3-carboxylate

[0563] ##STR00554##

[0564] Under argon atmosphere a mixture of (6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}pyridin-3-yl)methanol (1.2 g), DIPEA (1.8 mL) in DCM (5 mL) is treated dropwise with CH.sub.3SO.sub.2Cl (513 μL). The mixture is stirred for 15 minutes and then added dropwise to a mixture obtained by treatment of a solution of methyl 1H-pyrrole-3-carboxylate (863 mg) in DMF (15 mL) with KOtBu (893 mg). The mixture thus obtained is stirred for 5 days atrt. Then the mixture is partitioned between saturated aqueous NaHCO.sub.3 and EtOAc. The aqueous phase is extracted twice with EtOAc. The combined organic phases are dried (MgSO.sub.4), concentrated in vacuo and purified by HPLC on reversed phase (ACN, water) to give the title compounds.

[0565] Methyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}pyridin-3-yl)methyl]-1H-pyrrole-3-carboxylate (Intermediate 82): LC (Method 2): t.sub.R=0.71 min; Mass spectrum (ESI+): m/z=334 [M+H].sup.+.

[0566] Methyl 5-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}pyridin-3-yl)methyl]-1H-pyrrole-3-carboxylate (Intermediate 83): LC (Method 2): t.sub.R=0.70 min; Mass spectrum (ESI+): m/z=334 [M+H].sup.+.

Intermediate 84

Methyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3-yl)methyl]-1H-pyrrole-3-carboxylate and

Intermediate 85

Methyl 5-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3-yl)methyl]-1H-pyrrole-3-carboxylate

[0567] ##STR00555##

[0568] Under argon atmosphere a mixture of (6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3-yl)methanol (1.1 g), DIPEA (1.2 mL) in DCM (15 mL) is treated dropwise with CH.sub.3SO.sub.2Cl (656 μL). The mixture is stirred for 15 minutes and then added dropwise to a mixture obtained by treatment of a solution of methyl 1H-pyrrole-3-carboxylate (745 mg) in DMF (30 mL) with KOtBu (771 mg). The mixture thus obtained is stirred for 1 h at rt. Then the mixture is partitioned between saturated aqueous NaHCO.sub.3 and DCM. The aqueous phase is extracted twice with DCM. The combined organic phases are dried (MgSO.sub.4), concentrated in vacuo and the residue is chromatographed on silica gel (petroleum ether/EtOAc 90:10.fwdarw.50:50) to give the title compound.

[0569] Methyl 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3-yl)methyl]-1H-pyrrole-3-carboxylate (Intermediate 84): LC (Method 2): t.sub.R=0.74 min; Mass spectrum (ESI+): m/z=348 [M+H].sup.+.

[0570] Methyl 5-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3-yl)methyl]-1H-pyrrole-3-carboxylate (Intermediate 85): LC (Method 2): t.sub.R=0.73 min; Mass spectrum (ESI+): m/z=348 [M+H].sup.+.

Intermediate 86

Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-chloropyridin-3-yl)methyl]-1,2-oxazole-5-carboxylate

[0571] ##STR00556##

[0572] A mixture of 3-[6-chloro-5-(2-nitroethyl)pyridin-2-yl]-3-azabicyclo[3.1.0]hexane (2.58 g), Boc.sub.2O (4.42 g), ethyl propiolate (3.03 mL), DMAP (176 mg), and ACN (60 mL) is stirred for 18 h at 23° C. Purification by HPLC on reversed phase (ACN, water) gives the title compound. LC (Method 1): t.sub.R=1.15 min; Mass spectrum (ESI+): m/z=348[M+H].sup.+.

[0573] Intermediates 86-1 to 86-2 are prepared in analogy to Intermediate 86:

TABLE-US-00137 Mass spectrum (ESI+): Inter- m/z LC mediate Structure t.sub.R [M + H].sup.+ Method 86-1 [00557] embedded image 1.11 384 Method 1 86-2 [00558] 1.14 348 Method 2

TABLE-US-00138 Intermediate Name Name of Starting Material 86-1 Ethyl 3-[(2-chloro-6-{6,6-difluoro-3-azabicyclo[3.1.0]- 3-[6-Chloro-5-(2-nitroethyl)pyridin-2-yl]- hexan-3-yl}pyridin-3-yl)-methyl]-1,2-oxazole-5- 6,6-difluoro-3-azabicyclo[3.1.0]hexane carboxylate 86-2 Ethyl 3-[(6-{5-azaspiro[2.3]hexan-5-yl}-2- 5-[6-Chloro-5-(2-nitroethyl)pyridin-2-yl]- chloropyridin-3-yl)methyl]-1,2-oxazole-5-carboxylate 5-azaspiro[2.3]hexane

Intermediate 87

3-[6-Chloro-5-(2-nitroethyl)pyridin-2-yl]-3-azabicyclo[3.1.0]hexane

[0574] ##STR00559##

[0575] A mixture of 3-{6-chloro-5-[(1E)-2-nitroethenyl]pyridin-2-yl}-3-azabicyclo[3.1.0]hexane (4.0 g), NaBH.sub.4 (726 mg), acetic acid (5 mL), and DMSO (30 mL) is stirred for 30 min at 0° C., and for 1 h at rt. The mixture is diluted with water and EtOAc and the phases are separated. The aqueous phase is extracted three times with EtOAc. The combined organic phases are washed with brine, dried (MgSO.sub.4), concentrated and the residue is chromatographed on silica gel (cyclohexane/EtOAc 100:0.fwdarw.30:70) to give the title compound.

[0576] LC (Method 2): t.sub.R=1.10 min. Mass spectrum (ESI+): m/z=268 [M+H].sup.+.

[0577] Intermediates 87-1 to 87-2 are prepared in analogy to Intermediate 87:

TABLE-US-00139 Mass spectrum (ESI+): Inter- m/z LC mediate Structure t.sub.R [M + H].sup.+ Method 87-1 [00560] embedded image 1.07 304 Method 2 87-2 [00561] 1.07 268 Method 2

TABLE-US-00140 Intermediate Name Name of Starting Material 87-1 3-[6-Chloro-5-(2-nitroethyl)pyridin-2-yl]-6,6- 3-{6-Chloro-5-[(1E)-2-nitroethenyl]pyridin-2- difluoro-3-azabicyclo[3.1.0]hexane yl}-6,6-difluoro-3-azabicyclo[3.1.0]hexane 87-2 5-[6-Chloro-5-(2-nitroethyl)pyridin-2-yl]-5- 5-{6-Chloro-5-[(1E)-2-nitroethenyl]pyridin-2- azaspiro[2.3]hexane yl}-5-azaspiro[2.3]hexane

Intermediate 88

3-{6-Chloro-5-[(1E)-2-nitroethenyl]pyridin-2-yl}-3-azabicyclo[3.1.0]hexane

[0578] ##STR00562##

[0579] A mixture of 6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-chloropyridine-3-carbaldehyde (500 mg), nitromethane (1.6 mL), ammonium acetate (346 mg), and acetic acid (10 mL) is stirred for 18 h at 100° C. The mixture is cooled to rt and added dropwise to a cold mixture of EtOAc, water, and saturated aqueous NaHCO.sub.3. The phases are separated, and the aqueous phase is extracted three times with EtOAc. The combined organic phases are washed with brine, dried (MgSO.sub.4) and concentrated to give the crude title compound.

[0580] LC (Method 2): t.sub.R=1.14 min. Mass spectrum (ESI+): m/z=266 [M+H].sup.+.

[0581] Intermediates 88-1 to 88-2 are prepared in analogy to Intermediate 88:

TABLE-US-00141 Mass spectrum (ESI+): Inter- m/z LC mediate Structure t.sub.R [M + H].sup.+ Method 88-1 [00563] embedded image 1.10 302 Method 2 88-2 [00564] 1.11 266 Method 2

TABLE-US-00142 Intermediate Name Name of Starting Material 88-1 3-{6-Chloro-5-[(1E)-2-nitroethenyl]pyridin-2- 2-Chloro-6-{6,6-difluoro-3-azabicyclo[3.1.0]- yl}-6,6-difluoro-3-azabicyclo[3.1.0]hexane hexan-3-yl}pyridine-3-carbaldehyde 88-2 5-{6-Chloro-5-[(1E)-2-nitroethenyl]pyridin-2- 6-{5-Azaspiro[2.3]hexan-5-yl}-2- yl}-5-azaspiro[2.3]hexane chloropyridine-3-carbaldehyde

Intermediate 89

Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-ethylpyridin-3-yl)methyl]-1,2-oxazole-5-carboxylate

[0582] ##STR00565##

[0583] A mixture of (E,Z)-N-[2-(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-ethylpyridin-3-yl)ethylidene]hydroxylamine (2.45 g), ethyl prop-2-ynoate (2.0 mL), aqueous NaOCl 15% (34 mL), and THF (20 mL) is stirred for 3 h at rt. The mixture is diluted with EtOAc and water, and the aqueous phase is extracted three times with EtOAc. The combined organic phases are washed with brine, dried (MgSO.sub.4), concentrated and the residue is chromatographed on silica gel (petroleum ether/EtOAc 99:1.fwdarw.70:30) to give the title compound.

[0584] LC (Method 2): t.sub.R=0.79 min. Mass spectrum (ESI+): m/z=342 [M+H].sup.+.

[0585] Intermediates 89-1 to 89-2 are prepared in analogy to Intermediate 89:

TABLE-US-00143 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M+H].sup.+ LC Method 89-1 [00566] embedded image 1.04 314 Method 1 89-2 [00567] 0.76 364 Method 2

TABLE-US-00144 Intermediate Reaction comment 89-1 The reaction is conducted in DCM instead of THF.

TABLE-US-00145 Intermediate Name Name of Starting Material 89-1 Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}- (E,Z)-N-[2-(6-{3-Azabicyclo[3.1.0]hexan-3- pyridin-3-yl)methyl]-1,2-oxazole-5-carboxylate yl}pyridin-3-yl)ethylidene]hydroxylamine 89-2 Ethyl 3-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- (E,Z)-N-[2-(6-{6,6-Difluoro-3-azabicyclo[3.1.0]- hexan-3-yl}-2-methylpyridin-3-yl)methyl]-1,2- hexan-3-yl}-2-methylpyridin-3-yl)- oxazole-5-carboxylate ethylidene]hydroxylamine

Intermediate 90

(E,Z)-N-[2-(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-ethylpyridin-3-yl)ethylidene]hydroxylamine

[0586] ##STR00568##

[0587] A mixture of 2-(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-ethylpyridin-3-yl)acetaldehyde (2.22 g), hydroxylamine hydrochloride (1.35 g), Na.sub.2CO.sub.3 (1.23 g) water (8.0 mL), and MeOH (40 mL) is stirred for 2 h at rt. The mixture is concentrated, and the residue is treated with water, stirred for 15 minutes, and filtered. The precipitate is washed with water and dried in a desiccator, to give the title compound as a mixture of isomers.

[0588] LC (Method 2): t.sub.R=0.62 and 0.64 min. Mass spectrum (ESI+): m/z=246 [M+H].sup.+.

[0589] Intermediates 90-1 to 90-3 are prepared in analogy to Intermediate 90:

TABLE-US-00146 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M+H].sup.+ LC Method 90-1 [00569] embedded image 0.82 and 0.84 218 Method 1 90-2 [00570] 0.61 and 0.63 268 Method 2 90-3 [00571] 0.65 282 Method 2

TABLE-US-00147 Intermediate Name Name of Starting Material 90-1 (E,Z)-N-[2-(6-{3-Azabicyclo[3.1.0]hexan-3- 2-(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-pyridin- yl}pyridin-3-yl)ethylidene]hydroxylamine 3-yl)acetaldehyde 90-2 (E,Z)-N-[2-(6-{6,6-Difluoro-3- 2-(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan- azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3- 3-yl}-2-methylpyridin-3-yl)acetaldehyde yl)ethylidene]hydroxylamine 90-3 (E,Z)-N-[2-(6-{6,6-Difluoro-3- 2-(6-{6,6-Difluoro-3-azabicydo[3.1.0]hexan- azabicyclo[3.1.0]hexan-3-yl}-2-ethylpyridin-3- 3-yl}-2-ethylpyridin-3-yl)acetaldehyde yl)ethylidene]hydroxylamine

Intermediate 91

2-(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-ethylpyridin-3-yl)acetaldehyde

[0590] ##STR00572##

[0591] A mixture of 3-[6-ethyl-5-(2-methoxyethenyl)pyridin-2-yl]-3-azabicyclo[3.1.0]hexane (2.36 g), concentrated HCl (4.0 mL), and 1,4-dioxane (24 mL) is stirred for 1 h at rt. The mixture is carefully neutralized with a saturated aqueous solution of NaHCO.sub.3, and the aqueous phase is extracted twice with EtOAc. The combined organic phases are washed with brine, dried (MgSO.sub.4) and concentrated, to give the title compound.

[0592] LC (Method 2): t.sub.R=0.58 min. Mass spectrum (ESI+): m/z=231 [M+H].sup.+.

[0593] Intermediates 91-1 to 91-3 are prepared in analogy to Intermediate 91:

TABLE-US-00148 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M+H].sup.+ LC Method 91-1 [00573] embedded image 0.83 203 Method 1 91-2 [00574] 0.60 253 Method 2 91-3 [00575] 0.62 267 Method 2

TABLE-US-00149 Intermediate Name Name of Starting Material 91-1 2-(6-{3-Azabicyclo[3.1.0]hexan-3-yl}pyridin-3- 3-[5-(2-Methoxyethenyl)pyridin-2-yl]-3- yl)acetaldehyde azabicyclo[3.1.0]hexane 91-2 2-(6-{6,6-Difluoro-3-azabicydo[3.1.0]hexan-3- 6,6-Difluoro-3-[5-(2-methoxyethenyl)-6- yl}-2-methylpyridin-3-yl)acetaldehyde methylpyridin-2-yl]-3-azabicyclo[3.1.0]hexane 91-3 2-(6-{6,6-Difluoro-3-azabicydo[3.1.0]hexan-3- 3-[6-Ethyl-5-(2-methoxyethenyl)pyridin-2-yl]- yl}-2-ethylpyridin-3-yl)acetaldehyde 6,6-difluoro-3-azabicydo[3.1.0]hexane

Intermediate 92

3-[6-Ethyl-5-(2-methoxyethenyl)pyridin-2-yl]-3-azabicyclo[3.1.0]hexane

[0594] ##STR00576##

[0595] A mixture of (methoxymethyl)triphenylphosphonium chloride (10.0 g) in THF (80 mL) is treated dropwise with NaHMDS (2 M in THF, 14.6 mL), at −40° C. under argon atmosphere, and stirred for 15 minutes at this temperature. This mixture is treated dropwise with a mixture of 6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-ethylpyridine-3-carbaldehyde (2.09 g) in THF (20 mL) at −40° C. Then the mixture is warmed over 4 h to rt. The mixture is diluted with EtOAc, and the organic layer is washed with water and brine, dried (MgSO.sub.4), and concentrated. The residue is stirred in diisopropylether and the precipitate is filtered off. The filtrate is concentrate, and the residue is chromatographed on silica gel (petroleum ether/EtOAc 99:1.fwdarw.70:30) to give the title compound as a mixture of isomers.

[0596] LC (Method 2): t.sub.R=0.74 and 0.76 min (mixture of isomers). Mass spectrum (ESI+): m/z=245 [M+H].sup.+.

[0597] Intermediates 92-1 to 92-3 are prepared in analogy to Intermediate 92:

TABLE-US-00150 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M+H].sup.+ LC Method 92-1 [00577] embedded image 0.99 and 1.01 217 Method 1 92-2 [00578] 0.73 and 0.74 267 Method 2 92-3 [00579] 0.75 281 Method 2

TABLE-US-00151 Intermediate Name Name of Starting Material 92-1 3-[5-(2-Methoxyethenyl)pyridin-2-yl]-3- 6-{3-Azabicydo[3.1.0]hexan-3-yl}pyridine-3- azabicyclo[3.1.0]hexane carbaldehyde 92-2 6,6-Difluoro-3-[5-(2-methoxyethenyl)-6- 6-{6,6-Difluoro-3-azabicydo[3.1.0]hexan-3-yl}- methylpyridin-2-yl]-3-azabicyclo[3.1.0]hexane 2-methylpyridine-3-carbaldehyde 92-3 3-[6-Ethyl-5-(2-methoxyethenyl)pyridin-2-yl]- 6-{6,6-Difluoro-3-azabicydo[3.1.0]hexan-3-yl}- 6,6-difluoro-3-azabicyclo[3.1.0]hexane 2-ethylpyridine-3-carbaldehyde

Intermediate 93

Methyl 2-[1-(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3-yl)-2-methoxy-2-oxoethyl]-1,3-oxazole-5-carboxylate

[0598] ##STR00580##

[0599] A mixture of methyl 2-(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3-yl)acetate (460 mg) in THF (5 mL) is treated dropwise with NaHMDS (2M in THF, 1.1 mL), at −78° C. under argon atmosphere and is stirred for 15 minutes at this temperature. This mixture is treated dropwise with a mixture of methyl 2-chloro-1,3-oxazole-5-carboxylate (305 mg) in THF (3 mL) at −78° C. and is then warmed over 18 h to rt. The mixture is quenched with saturated aqueous NH.sub.4Cl and the aqueous phase is extracted twice with EtOAc. The combined organic phases are dried (MgSO.sub.4), concentrated and the residue is chromatographed on silica gel (petroleum ether/EtOAc 80:20.fwdarw.20:80) to give the title compound. LC (Method 2): t.sub.R=0.72 min. Mass spectrum (ESI+): m/z=372 [M+H].sup.+.

[0600] Intermediates 93-1 to 93-2 are prepared in analogy to Intermediate 93:

TABLE-US-00152 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M+H].sup.+ LC Method 93-1 [00581] embedded image 0.75 408 Method 2 93-2 [00582] 0.77 386 Method 2

TABLE-US-00153 Intermediate Name Name of Starting Material 93-1 Methyl 2-[1-(6-{6,6-difluoro-3-azabicyclo[3.1.0]- Methyl 2-(6-{6,6-difluoro-3-azabicyclo[3.1.0]- hexan-3-yl}-2-methylpyridin-3-yl)-2-methoxy-2- hexan-3-yl}-2-methylpyridin-3-yl)acetate oxoethyl]-1,3-oxazole-5-carboxylate 93-2 Methyl 2-[1-(6-{3-azabicyclo[3.1.0]hexan-3-yl}- Methyl 2-(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- 2-ethylpyridin-3-yl)-2-methoxy-2-oxoethyl]-1,3- ethylpyridin-3-yl)acetate oxazole-5-carboxylate

Intermediate 94

Methyl 2-(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3-yl)acetate

[0601] ##STR00583##

[0602] A mixture of 2-(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3-yl)acetonitrile (473 mg) in MeOH (5 mL) is treated with SOCl.sub.2 (575 μL) at rt, and the mixture is stirred for 6 h, before being treated with saturated aqueous NaHCO.sub.3. The aqueous phase is extracted twice with DCM, and the combined organic layers are washed with brine, dried (MgSO.sub.4) and concentrated to give the title compound. LC (Method 2): t.sub.R=0.64 min. Mass spectrum (ESI+): m/z=247 [M+H].sup.+.

[0603] Intermediates 94-1 to 94-2 are prepared in analogy to Intermediate 94:

TABLE-US-00154 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M+H].sup.+ LC Method 94-1 [00584] embedded image 0.66 283 Method 2 94-2 [00585] 0.69 261 Method 2

TABLE-US-00155 Intermediate Reaction comment 94-1 The reaction is conducted for 18 h at 50° C. 94-2 The reaction is conducted for 24 h.

TABLE-US-00156 Intermediate Name Name of Starting Material 94-1 Methyl 2-(6-{6,6-difluoro-3-azabicyclo[3.1.0]- 2-(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- hexan-3-yl}-2-methylpyridin-3-yl)acetate yl}-2-methylpyridin-3-yl)acetonitrile 94-2 Methyl 2-(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- 2-(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- ethylpyridin-3-yl)acetate ethylpyridin-3-yl)acetonitrile

Intermediate 95

2-(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3-yl)acetonitrile

[0604] ##STR00586##

[0605] A mixture of (6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3-yl)methanol (1.96 g) in THF (15 mL) is treated with 2-hydroxy-2-methylpropionitrile (895 mg), triphenylphosphine (PPh.sub.3, 3.80 g), and DIAD (2.36 g), at 0° C. The mixture is stirred for 66 h at rt and quenched with a saturated aqueous solution of NaHCO.sub.3. The aqueous phase is extracted with EtOAc. The organic phase is washed with brine, dried (MgSO.sub.4), concentrated and the residue is chromatographed on silica gel (petroleum ether/EtOAc 90:10.fwdarw.80:20) to give the title compound.

[0606] LC (Method 1: t.sub.R=0.97 min. Mass spectrum (ESI+): m/z=214 [M+H].sup.+.

[0607] Intermediates 95-1 to 95-2 are prepared in analogy to Intermediate 95:

TABLE-US-00157 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M+H].sup.+ LC Method 95-1 [00587] embedded image 0.95 250 Method 1 95-2 [00588] 0.63 228 Method 2

TABLE-US-00158 Intermediate Reaction comment 95-1 The reaction is conducted for 18 h at rt. 95-2 The reaction is conducted for 12 h at rt.

TABLE-US-00159 Intermediate Name Name of Starting Material 95-1 2-(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- (6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3- yl}-2-methylpyridin-3-yl)acetonitrile yl}-2-methylpyridin-3-yl)methanol 95-2 2-(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- (6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- ethylpyridin-3-yl)acetonitrile ethylpyridin-3-yl)methanol

Intermediate 96

Ethyl 3-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-ethylpyridin-3-yl)methyl]-1,2-oxazole-5-carboxylate

[0608] ##STR00589##

[0609] A mixture of N-[2-(6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-ethylpyridin-3-yl)ethylidene]hydroxylamine (100 mg), ethyl prop-2-ynoate (47 μL), oxone (328 mg), Na.sub.2CO.sub.3 (57 mg), NaCl (23 mg), MeOH (2 mL) and water (100 μL) is stirred for 5 h at rt, and concentrated. The residue is partitioned between water and EtOAc. The organic phase is washed with brine, dried (MgSO.sub.4), concentrated and the residue is chromatographed on silica gel (petroleum ether/EtOAc 99:01.fwdarw.50:50) to give the title compound.

[0610] LC (Method 2): t.sub.R=0.81 min. Mass spectrum (ESI+): m/z=378 [M+H].sup.+.

Intermediate 97

Methyl 5-[(2-chloro-6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}pyridin-3-yl)methyl]-1H-pyrrole-3-carboxylate

[0611] ##STR00590##

[0612] Under argon atmosphere a mixture of (2-chloro-6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}pyridin-3-yl)methanol (300 mg), DIPEA (782 μL) in DCM (1.5 mL) is treated dropwise with CH.sub.3SO.sub.2Cl (222 μL). The mixture is stirred for 15 minutes and then added dropwise to a mixture obtained by treatment of a solution of methyl 1H-pyrrole-3-carboxylate (187 mg) in DMF (4 mL) with KOtBu (194 mg). The mixture thus obtained is stirred for 12 h at 40° C. Then the mixture is partitioned between saturated aqueous NaHCO.sub.3 and EtOAc. The aqueous phase is extracted twice with EtOAc. The combined organic phases are dried (MgSO.sub.4), concentrated in vacuo and the residue is purified by HPLC on reversed phase (AON, water) to give the title compound.

[0613] LC (Method 2): t.sub.R=1.05 min; Mass spectrum (ESI+): m/z=368 [M+H].sup.+.

Intermediate 98

Ethyl 5-iodo-1H-pyrazole-3-carboxylate

[0614] ##STR00591##

[0615] Ethyl 5-amino-1H-pyrazole-3-carboxylate (10 g) is added portionwise at 0° C. to a mixture of water (240 mL) and concentrated aqueous H.sub.2SO.sub.4 (120 mL). To this mixture is added dropwise a solution of NaNO.sub.2 (4.65 g) in water (10 mL). The mixture is stirred for 2 h and is then treated dropwise with a solution of Kl (12.0 g) in water (10 mL). The mixture is stirred for 3 h while warming to rt. Then the mixture is cooled to 0° C. and neutralized by careful addition of saturated aqueous K.sub.2CO.sub.3. The mixture is extracted twice with EtOAc. The combined organic phases are washed with 20% Na.sub.2S.sub.2O.sub.3 in water, dried (MgSO.sub.4), concentrated and the residue is chromatographed on silica gel (petroleum ether/EtOAc 95:5.fwdarw.70:30) to give the title compound.

[0616] LC (Method 2): t.sub.R=0.86 min. Mass spectrum (ESI+): m/z=267 [M+H].sup.+.

[0617] Intermediate 98-1 is prepared in analogy to Intermediate 98:

TABLE-US-00160 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M+H].sup.+ LC Method 98-1 [00592] embedded image 1.02 281 Method 2

TABLE-US-00161 Intermediate Name Name of Starting Material 98-1 Ethyl 3-iodo-1-methyl- Ethyl 3-amino-1-methyl- 1H-pyrazole-5-carboxylate 1H-pyrazole-5-carboxylate hydrochloride

Intermediate 99

Ethyl 3-iodo-1-(propan-2-yl)-1H-pyrazole-5-carboxylate

[0618] ##STR00593##

[0619] NaH (60% in mineral oil, 180 mg) is added portionwise at 0° C. to a mixture of ethyl 5-iodo-1H-pyrazole-3-carboxylate (1.0 g) in DMF (15 mL). The mixture is stirred for 30 minutes and then treated with 2-iodopropane (451 μL). The mixture is stirred for 4 h while warming to rt. Then the mixture is partitioned between saturated aqueous NH.sub.4Cl and EtOAc. The aqueous phase is extracted twice with EtOAc. The combined organic phases are dried (MgSO.sub.4), concentrated in vacuo and the residue is purified by HPLC on reversed phase (ACN, water) to give the title compound.

[0620] LC (Method 2): t.sub.R=1.08 min. Mass spectrum (ESI+): m/z=309 [M+H].sup.+.

[0621] Intermediates 99-1 to 99-2 are prepared in analogy to Intermediate 99:

TABLE-US-00162 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M+H].sup.+ LC Method 99-1 [00594] embedded image 1.02 295 Method 2 99-2 [00595] 1.24 397 Method 2

TABLE-US-00163 Intermediate Reaction comment 99-1 lodoethane is used instead of 2-iodopropane. 99-2 (2-Chloromethoxy-ethyl)-trimethyl-silane (SEM-CI) is used instead of 2-iodopropane.

TABLE-US-00164 Intermediate Name Name of Starting Material 99-1 Ethyl 1-ethyl-3-iodo-1H-pyrazole-5-carboxylate Ethyl 5-iodo-1H-pyrazole-3-carboxylate 99-2 Ethyl 3-iodo-1-{[2-(trimethylsilyl)ethoxy]methyl}- Ethyl 5-iodo-1H-pyrazole-3-carboxylate 1H-pyrazole-5-carboxylate

Intermediate 100

Ethyl 3-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-ethylpyridin-3-yl)(hydroxy)methyl]-1-(propan-2-yl)-1H-pyrazole-5-carboxylate

[0622] ##STR00596##

[0623] A mixture of ethyl 3-iodo-1-(propan-2-yl)-1H-pyrazole-5-carboxylate (100 mg) in THF (2 mL) is treated dropwise at −40° C. under argon atmosphere with iPrMgCl×LiCl (1.3 M in THF, 300 μL). The mixture is stirred for 30 minutes and then treated dropwise with a mixture of 6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-ethylpyridine-3-carbaldehyde (90 mg) in THF (2 mL). After stirring for 3 h at −40° C. the mixture is partitioned between saturated aqueous NH.sub.4Cl and EtOAc. The aqueous phase is extracted with EtOAc. The combined organic phases are dried (MgSO.sub.4) and concentrated in vacuo to give the crude product, which is directly used in the next step.

[0624] LC (Method 2): t.sub.R=0.85 min. Mass spectrum (ESI+): m/z=435 [M+H].sup.+.

[0625] Intermediates 100-1 to 100-8 are prepared in analogy to Intermediate 100:

TABLE-US-00165 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M+H].sup.+ LC Method 100-1 [00597] embedded image 0.81 421 Method 2 100-2 [00598] 1.20 493 Method 2 100-3 [00599] 0.78 383 Method 2 100-4 [00600] 0.77 407 Method 2 100-5 [00601] 0.71 371 Method 2 100-6 [00602] 0.94 487 Method 2 100-7 [00603] 0.77 371 Method 2 100-8 [00604] 357

TABLE-US-00166 Intermediate Reaction comment 100-2 The aldehyde is added to the reaction mixture at −78° C., then the mixture is stirred for 1 h while warming to rt. The crude reaction product is purified by chromatography on silica gel (petroleum ether/EtOAc 99:1 .fwdarw. 50:50). 100-3 The reaction is conducted for 30 minutes at −40° C. and for 30 minutes at rt. 100-4 The reaction is conducted for 30 minutes at −40° C. and for 30 minutes at rt. 100-5 The reaction is conducted for 30 minutes at −40° C. and for 30 minutes at rt. 100-6 The aldehyde is added to the reaction mixture at −78° C., then the mixture is stirred for 1 h while warming to rt. The crude reaction product is purified by chromatography on silica gel (petroleum ether/EtOAc 90:10 .fwdarw. 70:30). 100-7 The aldehyde is added to the reaction mixture at −78° C., then the mixture is stirred for 1 h while warming to rt. The crude reaction product is purified by chromatography on silica gel (petroleum ether/EtOAc 60:40 .fwdarw. 0:100). 100-8 The aldehyde is added to the reaction mixture at −78° C., then the mixture is stirred for 2 h while warming to rt. The crude reaction product is purified by chromatography on silica gel (cyclohexane/ EtOAc 90:10 .fwdarw. 50:50).

TABLE-US-00167 Name of Name of Intermediate Name Starting Material 1 Starting Material 2 100-1 Ethyl 3-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- Ethyl 1-ethyl-3-iodo- 6-{6,6-Difluoro-3-azabicyclo- hexan-3-yl}-2-ethylpyridin-3-yl)(hydroxy)- 1H-pyrazole-5- [3.1.0]hexan-3-yl}-2-ethyl- methyl]-1-ethyl-1H-pyrazole-5-carboxylate carboxylate pyridine-3-carbaldehyde 100-2 Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}- Ethyl 3-iodo-1-{[2- 6-{3-Azabicyclo[3.1.0]hexan- 2-chloropyridin-3-yl)(hydroxy)-methyl]-1- (trimethylsilyl)ethoxy]methyl}- 3-yl}-2-chloropyridine-3- {[2-(trimethylsilyl)ethoxy]-methyl}-1H- 1H-pyrazole-5- carbaldehyde pyrazole-5-carboxylate carboxylate 100-3 Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}- Ethyl 1-ethyl-3-iodo- 6-{3-Azabicyclo[3.1.0]hexan- 2-ethenylpyridin-3-yl)(hydroxy)-methyl]-1- 1H-pyrazole-5- 3-yl}-2-ethenylpyridine-3- ethyl-1H-pyrazole-5-carboxylate carboxylate carbaldehyde 100-4 Ethyl 3-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- Ethyl 1-ethyl-3-iodo- 6-{6,6-Difluoro-3-azabicyclo- hexan-3-yl}-2-methylpyridin-3-yl)(hydroxy)- 1H-pyrazole-5- [3.1.0]hexan-3-yl}-2-methyl- methyl]-1-ethyl-1H-pyrazole-5-carboxylate carboxylate pyridine-3-carbaldehyde 100-5 Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}- Ethyl 1-ethyl-3-iodo- 6-{3-Azabicyclo[3.1.0]hexan- 2-methylpyridin-3-yl)(hydroxy)-methyl]-1- 1H-pyrazole-5- 3-yl}-2-methylpyridine-3- ethyl-1H-pyrazole-5-carboxylate carboxylate carbaldehyde 100-6 Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}- Ethyl 3-iodo-1-{[2- 6-{3-Azabicyclo[3.1.0]hexan- 2-ethylpyridin-3-yl)(hydroxy)methyl]-1-{[2- (trimethylsilyl)ethoxy]methyl}- 3-yl}-2-ethylpyridine-3- (trimethylsilyl)ethoxy]methyl}-1H-pyrazole- 1H-pyrazole-5- carbaldehyde 5-carboxylate carboxylate 100-7 Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}- Ethyl 3-iodo-1-methyl- 6-{3-Azabicyclo[3.1.0]- 2-ethylpyridin-3-yl)(hydroxy)methyl]-1- 1H-pyrazole-5- hexan-3-yl}-2-ethylpyridine- methyl-1H-pyrazole-5-carboxylate carboxylate 3-carbaldehyde 100-8 Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}- Ethyl 3-iodo-1-methyl- 6-{3-Azabicyclo[3.1.0]- 2-methylpyridin-3-yl)(hydroxy)-methyl]-1- 1H-pyrazole-5- hexan-3-yl}-2-methyl- methyl-1H-pyrazole-5-carboxylate carboxylate pyridine-3-carbaldehyde

Intermediate 101

Ethyl 3-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-ethylpyridin-3-yl)methyl]-1-(propan-2-yl)-1H-pyrazole-5-carboxylate

[0626] ##STR00605##

[0627] A mixture of ethyl 3-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-ethylpyridin-3-yl)(hydroxy)methyl]-1-(propan-2-yl)-1H-pyrazole-5-carboxylate (141 mg), triethylsilane (492 μL) and trifluoroacetic acid (594 μL) in 1,2-dichloroethane (1.18 mL) is stirred under argon atmosphere for 30 minutes at rt. The mixture is concentrated in vacuo to give the crude product, which is directly used in the next step.

[0628] LC (Method 2): t.sub.R=0.90 min. Mass spectrum (ESI+): m/z=419 [M+H].sup.+.

[0629] Intermediates 101-1 to 101-12 are prepared in analogy to Intermediate 101:

TABLE-US-00168 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M+H].sup.+ LC Method 101-1 [00606] embedded image 0.86 405 Method 2 101-2 [00607] 0.84 367 Method 2 101-3 [00608] 0.82 391 Method 2 101-4 [00609] 0.82 355 Method 2 101-5 [00610] 0.74 313 Method 2 101-6 [00611] 1.19 327 Method 1 101-7 [00612] 0.77 349 Method 2 101-8 [00613] 1.12 413 Method 1 101-9 [00614] 0.85 355 Method 2 101-10 [00615] embedded image 1.22 443 Method 2 101-11 [00616] 0.84 379 Method 2 101-12 [00617] 0.78 386 Method 2

TABLE-US-00169 Intermediate Reaction comment 101-1 The reaction is conducted for 2 h at rt. 101-2 The reaction is conducted for 4 h at 30° C. 101-3 The reaction is conducted for 45 minutes at 30° C. 101-4 The reaction is conducted for 30 minutes at 30° C. 100-5 The reaction is conducted at 40° C. for 20 h. The product is purified by HPLC on reversed phase (ACN, water). 101-6 The reaction is conducted at 50° C. for 2 h. 101-7 The reaction is conducted at 50° C. for 30 minutes. 101-8 The reaction is conducted at 50° C. for 30 minutes. 101-9 The reaction is conducted at rt for 1.5 h. 101-10 The reaction is conducted at 30° C. for 2 h. 101-11 The reaction is conducted at 50° C. for 30 minutes. 101-12 The reaction is conducted at 70° C. for 2 h.

TABLE-US-00170 Intermediate Name Name of Starting Material 101-1 Ethyl 3-[(6-{6,6-difluoro-3- Ethyl 3-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- azabicyclo[3.1.0]hexan-3-yl}-2-ethylpyridin-3- hexan-3-yl}-2-ethylpyridin-3-yl)(hydroxy)- yl)methyl]-1-ethyl-1H-pyrazole-5-carboxylate methyl]-1-ethyl-1H-pyrazole-5-carboxylate 101-2 Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 3-[(6-{3-azabicydo[3.1.0]hexan-3-yl}-2- ethenylpyridin-3-yl)methyl]-1-ethyl-1H- ethenylpyridin-3-yl)(hydroxy)methyl]-1-ethyl- pyrazole-5-carboxylate 1H-pyrazole-5-carboxylate 101-3 Ethyl 3-[(6-{6,6-difluoro-3- Ethyl 3-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]- azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin- hexan-3-yl}-2-methylpyridin-3-yl)(hydroxy)- 3-yl)methyl]-1-ethyl-1H-pyrazole-5-carboxylate methyl]-1-ethyl-1H-pyrazole-5-carboxylate 101-4 Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 3-[(6-{3-azabicydo[3.1.0]hexan-3-yl}-2- methylpyridin-3-yl)methyl]-1-ethyl-1H- methylpyridin-3-yl)(hydroxy)methyl]-1-ethyl- pyrazole-5-carboxylate 1H-pyrazole-5-carboxylate 101-5 Methyl 5-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- Methyl 5-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- methylpyridin-3-yl)methyl]furan-2-carboxylate methylpyridin-3-yl)(hydroxy)methyl]furan-2- carboxylate 101-6 Ethyl 5-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 5-[(6-{3-azabicydo[3.1.0]hexan-3-yl}-2- methylpyridin-3-yl)methyl]furan-3-carboxylate methylpyridin-3-yl)(hydroxy)methyl]furan-3- carboxylate 101-7 Methyl 5-[(6-{6,6-difluoro-3- Methyl 5-[(6-{6,6-difluoro-3- azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin- azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin- 3-yl)methyl]furan-2-carboxylate 3-yl)(hydroxy)methyl]furan-2-carboxylate 101-8 Methyl 5-[(2-bromo-6-{6,6-difluoro-3- Methyl 5-[(2-bromo-6-{6,6-difluoro-3- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- yl)methyl]furan-2-carboxylate yl)(hydroxy)methyl]furan-2-carboxylate 101-9 Ethyl 5-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- Ethyl 5-[(6-{3-azabicydo[3.1.0]hexan-3-yl}-2- ethenylpyridin-3-yl)methyl]thiophene-2- ethenylpyridin-3-yl)(hydroxy)methyl]thiophene- carboxylate 2-carboxylate 101-10 Ethyl 5-[(2-bromo-6-{6,6-difluoro-3- Ethyl 5-[(2-bromo-6-{6,6-difluoro-3- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- yl)methyl]thiophene-2-carboxylate yl)(hydroxy)methyl]thiophene-2-carboxylate 101-11 Ethyl 5-[(6-{6,6-difluoro-3- Ethyl 5-[(6-{6,6-difluoro-3- azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin- azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin- 3-yl)methyl]thiophene-2-carboxylate 3-yl)(hydroxy)methyl]thiophene-2-carboxylate 101-12 3-{5-[(5-Bromo-1,3-thiazol-2-yl)methyl]-6- (5-Bromo-1,3-thiazol-2-yl)(6-{6,6-difluoro-3- methylpyridin-2-yl}-6,6-difluoro-3- azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin- azabicyclo[3.1.0]hexane 3-yl)methanol

Intermediate 102

Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-chloropyridin-3-yl)methyl]-1H-pyrazole-5-carboxylate

[0630] ##STR00618##

[0631] A mixture of ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-chloropyridin-3-yl)(hydroxy)methyl]-1-{[2-(trimethylsilyl)ethoxy]methyl}-1H-pyrazole-5-carboxylate (848 mg), triethylsilane (1.38 mL), trifluoroacetic acid (663 μL) and borontrifluoride-diethyletherate (BF.sub.3×OEt.sub.2, 2.3 mL) in DCM (8 mL) is stirred under argon atmosphere for 12 h at rt. The mixture is partitioned between water and DCM. The aqueous phase is extracted twice with DCM. The combined organic phases are washed with brine, dried (MgSO.sub.4), concentrated in vacuo and the residue is chromatographed on silica gel (petroleum ether/EtOAc 80:20.fwdarw.0:100) to give the title compound.

[0632] LC (Method 2): t.sub.R=1.05 min. Mass spectrum (ESI+): m/z=347 [M+H].sup.+.

[0633] Intermediates 102-1 to 102-3 are prepared in analogy to Intermediate 102:

TABLE-US-00171 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M+H].sup.+ LC Method 102-1 [00619] embedded image 0.75 341 Method 2 102-2 [00620] 0.82 355 Method 2 102-3 [00621] 1.15 341 Method 1

TABLE-US-00172 Intermediate Reaction comment 102-3 The reaction is conducted at 0° C. The mixture is stirred for 1.5 h while warming to rt.

TABLE-US-00173 Intermediate Name Name of Starting Material 102-1 Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3- Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-ethyl- yl}-2-ethylpyridin-3-yl)methyl]-1H- pyridin-3-yl)(hydroxy)methyl]-1-{[2-(trimethylsilyl)- pyrazole-5-carboxylate ethoxy]methyl}-1H-pyrazole-5-carboxylate 102-2 Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3- Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- yl}-2-ethylpyridin-3-yl)methyl]-1-methyl- ethylpyridin-3-yl)(hydroxy)methyl]-1-methyl-1H- 1H-pyrazole-5-carboxylate pyrazole-5-carboxylate 102-3 Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3- Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- yl}-2-methylpyridin-3-yl)methyl]-1- methylpyridin-3-yl)(hydroxy)methyl]-1-methyl-1H- methyl-1H-pyrazole-5-carboxylate pyrazole-5-carboxylate

Intermediate 103

Ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-chloropyridin-3-yl)methyl]-1-{[2-(trimethylsilyl)ethoxy]methyl}-1H-pyrazole-5-carboxylate and Ethyl 5-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-chloropyridin-3-yl)methyl]-1-({[2-(trimethylsilyl)ethyl]amino}methyl)-1-{[2-(trimethylsilyl)ethoxy]methyl}-1H-pyrazole-3-carboxylate (Mixture of Isomers)

[0634] ##STR00622##

[0635] NaH (60% in mineral oil, 73 mg) is added under argon atmosphere at 0° C. to a mixture of ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-chloropyridin-3-yl)methyl]-1H-pyrazole-5-carboxylate (500 mg) in DMF (3 mL). The mixture is stirred for 30 minutes and then treated dropwise with (2-chloromethoxy-ethyl)-trimethyl-silane (SEM-CI, 313 μL). The mixture is stirred for 2 h while warming to rt. Then the mixture is partitioned between saturated aqueous NH.sub.4Cl and EtOAc. The aqueous phase is extracted with EtOAc. The combined organic phases are washed with brine, dried (MgSO.sub.4), concentrated in vacuo and the residue is chromatographed on silica gel (petroleum ether/EtOAc 99:1.fwdarw.70:30) to give the title compounds as a mixture of isomers.

[0636] LC (Method 2): t.sub.R=1.05 min. Mass spectrum (ESI+): m/z=347 [M+H].sup.+.

Intermediate 104

Ethyl 3-[{6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-ethylpyridin-3-yl}methyl]-1-ethyl-1H-pyrazole-5-carboxylate

[0637] ##STR00623##

[0638] A mixture of ethyl 3-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-ethenylpyridin-3-yl)methyl]-1-ethyl-1H-pyrazole-5-carboxylate (40 mg), 10% palladium on carbon (5 mg) in MeOH (3 mL) is shaken under hydrogen atmosphere (3 bar) at rt for 4.5 h. The mixture is filtered and the filtrate is concentrated in vacuo to give the crude product, which is directly used in the next step. LC (Method 2): t.sub.R=0.84 min; Mass spectrum (ESI+): m/z=369 [M+H].sup.+.

[0639] Intermediates 104-1 is prepared in analogy to Intermediate 104:

TABLE-US-00174 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M+H].sup.+ LC Method 104-1 [00624] embedded image 0.75 357 Method 2

TABLE-US-00175 Intermediate Reaction comment 104-1 The reaction is conducted at rt for 4 h under 1 bar hydrogen atmosphere. The product is purified by HPLC on reversed phase (ACN, water).

TABLE-US-00176 Intermediate Name Name of Starting Material 104-1 Ethyl 5-[(6-{3-azabicydo[3.1.0]hexan-3-yl}-2- Ethyl 5-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2- ethylpyridin-3-yl)methyl]thiophene-2-carboxylate ethenylpyridin-3-yl)methyl]thiophene-2-carboxylate

Intermediate 105

Ethyl 3-[(6-fluoro-2-methylpyridin-3-yl)(hydroxy)methyl]-1-methyl-1H-pyrazole-5-carboxylate

[0640] ##STR00625##

[0641] A mixture of 6-fluoro-3-iodo-2-methylpyridine (550 mg) in THF (25 mL) is treated dropwise at −50° C. under argon atmosphere with iPrMgCl×LiCl (1.3 M in THF, 2.2 mL). The mixture is stirred for 1 h and then treated dropwise with a mixture of ethyl 3-formyl-1-methyl-1H-pyrazole-5-carboxylate (300 mg) in THF (1 mL). After stirring for 1 h at −50° C. the mixture is partitioned between saturated aqueous NH.sub.4Cl and EtOAc. The aqueous phase is extracted with EtOAc. The combined organic phases are dried (MgSO.sub.4), concentrated in vacuo and the residue is chromatographed on silica gel (petroleum ether/EtOAc 90:10.fwdarw.50:50) to give the title compound.

[0642] LC (Method 2): t.sub.R=0.89 min. Mass spectrum (ESI+): m/z=294 [M+H].sup.+.

Intermediate 106

3-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3-yl)(hydroxy)methyl]-1-methyl-1H-pyrazole-5-carboxylic acid

[0643] ##STR00626##

[0644] A mixture of ethyl 3-[(6-fluoro-2-methylpyridin-3-yl)(hydroxy)methyl]-1-methyl-1H-pyrazole-5-carboxylate (430 mg), 6,6-difluoro-3-azabicyclo[3.1.0]hexane hydrochloride (1.36 g) and K.sub.2CO.sub.3 (2.4 g) in DMSO (10 mL) is heated for 48 h to 150° C. After cooling to rt the mixture is diluted with ACN, filtered and purified by HPLC on reversed phase (ACN, water) to give the title compound. LC (Method 2): t.sub.R=0.63 min; Mass spectrum (ESI+): m/z=365 [M+H].sup.+.

Intermediate 107

3-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3-yl)methyl]-1-methyl-1H-pyrazole-5-carboxylic acid

[0645] ##STR00627##

[0646] A mixture of 3-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3-yl)(hydroxy)methyl]-1-methyl-1H-pyrazole-5-carboxylic acid (173 mg), triethylsilane (380 μL), trifluoroacetic acid (185 μL) and borontrifluoride-diethyletherate (BF.sub.3×OEt.sub.2, 293 μL) in DCM (3 mL) and THF (1 mL) is stirred under argon atmosphere for 12 h at rt. The mixture is diluted with water and purified by HPLC on reversed phase (ACN, water) to give the title compound.

[0647] LC (Method 2): t.sub.R=0.69 min. Mass spectrum (ESI+): m/z=349 [M+H].sup.+.

Intermediate 108

1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2-methylpyridin-3-yl)methyl]-1H-pyrazole-4-carboxylic acid

[0648] ##STR00628##

Step 1: Ethyl 1-[(6-fluoro-2-methylpyridin-3-yl)methyl]-1H-pyrazole-4-carboxylate

[0649] (6-Fluoro-2-methylpyridin-3-yl)methanol (4.12 g) is dissolved in THF (50 mL) and cooled to −10° C. Ethyl 1H-pyrazole-4-carboxylate (4.43 g) and tributyl phosphine (9 mL) are added. Di-tert.-butyl-azodicarboxylate (DBAD, 7.4 g) is slowly added portionwise, the mixture is stirred at rt for 45 min and concentrated in vacuo. The residue is chromatographed on silica gel (cyclohexane/EtOAc) to give the title compound.

[0650] LC (Method 2): t.sub.R=0.88 min; Mass spectrum (ESI.sup.+): m/z=264 [M+H].sup.+.

Step 2: 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2-methylpyridin-3-yl)methyl]-1 FI-pyrazole-4-carboxylic acid

[0651] Ethyl 1-[(6-fluoro-2-methylpyridin-3-yl)methyl]-1H-pyrazole-4-carboxylate (0.5 g) is dissolved in DMSO (2 mL). 5-Azaspiro[2.3]hexane trifluoroacetate (1.2 g) and DIPEA (2 mL) are added and the mixture is stirred for 16 h at 100° C. and additional 5 h at 120° C. After cooling to rt, the N,N-diisopropyl-ethylamine phase is removed, 4 M NaOH (4 mL) is added and stirred at 60° C. for 2 h. Aqueous HCl (4 M, 4 mL) is added and the mixture is purified by HPLC on reversed phase (ACN, water) to give the title compound. LC (Method 1): t.sub.R=0.68 min; Mass spectrum (ESI.sup.+): m/z=299 [M+H].sup.+.

Intermediate 109

Methyl 5-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3-yl)(hydroxy)methyl]furan-2-carboxylate

[0652] ##STR00629##

[0653] A mixture of methyl 5-bromofuran-2-carboxylate (500 mg) in THF (15 mL) is treated dropwise at −50° C. with iPrMgCl×LiCl (1.3 M in THF, 1.95 mL). The mixture is stirred at for 30 minutes at −50° C. and then cooled to −78° C. 6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridine-3-carbaldehyde (592 mg) in THF (8 mL) is added and the mixture is stirred for 1 h at −78° C. and then for 30 minutes at 0° C. The reaction is quenched with saturated aqueous NH.sub.4Cl and water. The mixture is extracted with EtOAc. The combined organic phases are dried (MgSO.sub.4), concentrated in vacuo and the residue is chromatographed on silica gel (petroleum ether/EtOAc 85:15.fwdarw.50:50) to give the title compound.

[0654] LC (Method 2): t.sub.R=0.70 min. Mass spectrum (ESI+): m/z=329 [M+H].sup.+.

[0655] Intermediates 109-1 to 109-7 are prepared in analogy to Intermediate 109:

TABLE-US-00177 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M+H].sup.+ LC Method 109-1 [00630] embedded image 1.05 343 Method 1 109-2 [00631] 0.97 365 Method 1 109-3 [00632] 1.04 429 Method 2 109-4 [00633] 0.80 371 Method 2 109-5 [00634] 1.12 459 Method 2 109-6 [00635] 0.807 395 Method 2 109-7 [00636] 0.74 402 Method 2

TABLE-US-00178 Intermediate Reaction comment 109-1 After addition of iPrMgClxLiCl the mixture is slowly warmed to −10° C. over 2 h. Then the mixture is cooled to −30° C. and the aldehyde is added, followed by stirring for 1 h while warming to −10° C. 109-3 The reaction is conducted at −78° C. The product is purified by HPLC on reversed phase (ACN, water). 109-4 The reaction is conducted at −60° C. instead of −50° C. and warmed after the addition to rt over 17 h. 109-5 The reaction is conducted at −78° C. 109-6 The reaction is conducted at −78° C. The product is purified by HPLC on reversed phase (ACN, water). 109-7 The reaction is conducted at −78° C. The product is purified by HPLC on reversed phase (ACN, water).

TABLE-US-00179 Inter- Name of Name of mediate Name Starting Material 1 Starting Material 2 109-1 Ethyl 5-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}- 3-(5-Iodo-6-methyl- Ethyl 5-formylfuran-3- 2-methylpyridin-3-yl)- pyridin-2-yl)-3-aza- carboxylate (hydroxy)methyl]furan-3-carboxylate bicyclo[3.1.0]hexane 109-2 Methyl 5-[(6-{6,6-difluoro-3-azabicyclo- Methyl 5-bromofuran- 6-{6,6-Difluoro-3-azabicyclo- [3.1.0]hexan-3-yl}-2-methyl-pyridin-3- 2-carboxylate [3.1.0]hexan-3-yl}-2-methyl- yl)(hydroxy)methyl]furan-2-carboxylate pyridine-3-carbaldehyde 109-3 Methyl 5-[(2-bromo-6-{6,6-difluoro-3- Methyl 5-bromofuran- 2-Bromo-6-{6,6-difluoro-3- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- 2-carboxylate azabicyclo[3.1.0]hexan-3- yl)(hydroxy)methyl]furan-2-carboxylate yl}pyridine-3-carbaldehyde 109-4 Ethyl 5-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}- Ethyl 5- 6-{3-Azabicyclo[3.1.0]hexan- 2-ethenylpyridin-3-yl)(hydroxy)- bromothiophene-2- 3-yl}-2-ethenylpyridine-3- methyl]thiophene-2-carboxylate carboxylate carbaldehyde 109-5 Ethyl 5-[(2-bromo-6-{6,6-difluoro-3- Ethyl 5- 2-Bromo-6-{6,6-difluoro-3- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- bromothiophene-2- azabicyclo[3.1.0]hexan-3- yl)(hydroxy)methyl]thiophene-2-carboxylate carboxylate yl}pyridine-3-carbaldehyde 109-6 Ethyl 5-[(6-{6,6-difluoro-3-azabicyclo- Ethyl 5- 6-{6,6-Difluoro-3-azabicyclo- [3.1.0]hexan-3-yl}-2-methylpyridin-3- bromothiophene-2- [3.1.0]hexan-3-yl}-2-methyl- yl)(hydroxy)methyl]-thiophene-2-carboxylate carboxylate pyridine-3-carbaldehyde 109-7 (5-Bromo-1,3-thiazol-2-yl)(6-{6,6-difluoro- 2,5-Dibromo-1,3- 6-{6,6-Difluoro-3-azabicyclo- 3-azabicyclo[3.1.0]hexan-3-yl}-2- thiazole [3.1.0]hexan-3-yl}-2-methyl- methylpyridin-3-yl)methanol pyridine-3-carbaldehyde

Intermediate 110

1-[(4-{3-Azabicyclo[3.1.0]hexan-3-yl}-3-cyanophenyl)methyl]-1H-pyrazole-4-carboxylic acid

[0656] ##STR00637##

Step 1: Methyl 4-{3-azabicyclo[3.1.0]hexan-3-yl}-3-cyanobenzoate

[0657] The title compound is prepared from methyl 3-cyano-4-fluorobenzoate and 3-azabicyclo[3.1.0]hexane hydrochloride following a procedure analogous to that described in Step 1 of Intermediate 111.

Step 2: 2-{3-Azabicyclo[3.1.0]hexan-3-yl}-5-(hydroxymethyl)benzonitrile

[0658] The title compound is prepared from methyl 4-{3-azabicyclo[3.1.0]hexan-3-yl}-3-cyanobenzoate following a procedure analogous to that described in Step 4 of Intermediate 118.

[0659] LC (Method 2): t.sub.R=0.92 min; Mass spectrum (ESP): m/z=215 [M+H].sup.+.

Step 3: Ethyl 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3-cyanophenyl)methyl]-1H-pyrazole-4-carboxylate

[0660] The title compound is prepared from 2-{3-azabicyclo[3.1.0]hexan-3-yl}-5-(hydroxymethyl)benzonitrile and ethyl 1H-pyrazole-4-carboxylate following a procedure analogous to that described in Step 3 of Intermediate 111.

Step 4: 1-[(4-{3-Azabicyclo[3.1.0]hexan-3-yl}-3-cyanophenyl)methyl]-1H-pyrazole-4-carboxylic acid

[0661] The title compound is prepared from ethyl 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3-cyanophenyl)methyl]-1H-pyrazole-4-carboxylate following a procedure analogous to that described in Step 4 of Intermediate 111.

Intermediate 111

1-[(4-{3-Azabicyclo[3.1.0]hexan-3-yl}-3,5-difluorophenyl)methyl]-1H-pyrazole-4-carboxylic acid

[0662] ##STR00638##

Step 1: 4-{3-Azabicyclo[3.1.0]hexan-3-yl}-3,5-difluorobenzaldehyde

[0663] A mixture of 3,4,5-trifluorobenzaldehyde (1.50 g), 3-azabicyclo[3.1.0]hexane hydrochloride (1.23 g), .sup.iPr.sub.2NEt (4 mL), and DMF (15 mL) is stirred at 70° C. overnight. After cooling to rt, water is added and the resulting mixture is extracted with ethyl acetate (3×). The combined extract is dried (Na.sub.2SO.sub.4) and concentrated. The residue is chromatographed on silica gel (petroleum ether/EtOAc 98:2.fwdarw.95:5) to give the title compound.

[0664] LC (Method 2): t.sub.R=1.13 min; Mass spectrum (ESP): m/z=224 [M+H].sup.+.

Step 2: (4-{3-Azabicyclo[3.1.0]hexan-3-yl}-3,5-difluorophenyl)methanol

[0665] NaBH.sub.4 (0.18 g) is added portionwise to 4-{3-azabicyclo[3.1.0]hexan-3-yl}-3,5-difluorobenzaldehyde (0.97 g) in THF (10 mL) and methanol (10 mL) at 0° C. The mixture is stirred for 1 h in the cooling bath and another 30 min at rt before aqueous HCl solution (1 mol/L) is added. The mixture is stirred for 30 min before it is neutralized with aqueous NaFICCh solution. The mixture is extracted with ethyl acetate (2×), and the combined extract is dried (Na.sub.2SO.sub.4) and concentrated. The residue is chromatographed on silica gel (petroleum ether/EtOAc 95:5.fwdarw.85:15) to give the title compound.

[0666] LC (Method 2): t.sub.R=1.04 min; Mass spectrum (ESP): m/z=226 [M+H].sup.+.

Step 3: Ethyl 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3,5-difluorophenyl)methyl]-1H-pyrazole-4-carboxylate

[0667] A mixture of (4-{3-azabicyclo[3.1.0]hexan-3-yl}-3,5-difluorophenyl)methanol (0.62 g), ethyl 1 FI-pyrazole-4-carboxylate (0.48 g), p-toluenesulfonic acid (0.28 g), and MeCN (5 mL) is stirred at 70° C. for 1.5 h (if the reaction is not complete and depending on the degree of conversion the temperature is increased and/or reaction time is extended). After cooling to room temperature, the mixture is concentrated, water is added, and the resulting mixture is neutralized with aqueous NaHCO.sub.3 solution. The resulting mixture is extracted with ethyl acetate (3×), and the combined extract is dried (Na.sub.2SO.sub.4) and concentrated. The residue is chromatographed (HPLC; ACN/water/ammonia) to give the title compound. LC (Method 2): t.sub.R=1.20 min; Mass spectrum (ESP): m/z=348 [M+H].sup.+.

Step 4: 1-[(4-{3-Azabicyclo[3.1.0]hexan-3-yl}-3,5-difluorophenyl)methyl]-1H-pyrazole-4-carboxylic acid

[0668] A mixture of ethyl 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3,5-difluorophenyl)methyl]-1H-pyrazole-4-carboxylate (0.10 g), aqueous NaOH (4 mol/L; 0.5 mL), THF (2 mL), and EtOH (2 mL) is stirred at 70° C. for 1.5 h. After cooling to room temperature, the mixture is concentrated. Water (2 mL) and aqueous HCl (4 mol/L; 0.5 mL) are added, and the resulting mixture is adjusted to a pH value of ca. 5 with aq. NaOH. The precipitate formed is separated and dried and used as is in the next reaction step; alternatively, if no precipitate forms, the aqueous phase is concentrated and the remainder is used as is in the next reaction step.

[0669] LC (Method 2): t.sub.R=1.04 min; Mass spectrum (ESI.sup.+): m/z=320 [M+H].sup.+.

Intermediate 112

2-[(4-{3-Azabicyclo[3.1.0]hexan-3-yl}-3,5-difluorophenyl)methyl]-1H-imidazole-5-carboxylic acid

[0670] ##STR00639##

Step 1: 3-[4-(Chloromethyl)-2,6-difluorophenyl]-3-azabicyclo[3.1.0]hexane

[0671] A mixture of (4-{3-azabicyclo[3.1.0]hexan-3-yl}-3,5-difluorophenyl)methanol (60 mg), SOCl.sub.2 (0.04 mL), and dichloromethane (1 mL) is stirred at room temperature for 30 min. The mixture is concentrated, taken up in toluene, concentrated again, and used as is in the next reaction step.

Step 2: Ethyl 2-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3,5-difluorophenyl)methyl]-1H-imidazole-5-carboxylate

[0672] LiO.sup.tBu (21 mg) is added to a mixture of ethyl 1H-imidazole-4-carboxylate (37 mg), BuOH (0.4 mL), and DCM (1.6 mL) chilled in an ice bath. The mixture is stirred for 5 min prior to the addition of 3-[4-(chloromethyl)-2,6-difluorophenyl]-3-azabicyclo[3.1.0]hexane (65 mg, crude product from Step 1) in DCM (0.4 mL). The cooling bath is removed, and the mixture is stirred at 40° C. overnight. After cooling to rt, water and DCM are added. The organic phase is separated, and the aqueous phase is extracted with DCM (2×). The combined organic extract is concentrated, and the residue is chromatographed (HPLC; ACN/water/ammonia) to give the title compound.

[0673] LC (Method 2): t.sub.R=0.89 min; Mass spectrum (ESP): m/z=348 [M+H].sup.+.

Step 3: 2-[(4-{3-Azabicyclo[3.1.0]hexan-3-yl}-3,5-difluorophenyl)methyl]-1H-imidazole-5-carboxylic acid

[0674] The title compound is prepared from ethyl 2-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3,5-difluorophenyl)methyl]-1H-imidazole-5-carboxylate following a procedure analogous to that described in Step 4 of Intermediate 111.

[0675] LC (Method 2): t.sub.R=0.82 min; Mass spectrum (ESP): m/z=320 [M+H].sup.+.

Intermediate 113

1-[(4-{3-Azabicyclo[3.1.0]hexan-3-yl}-3-cyano-2-methylphenyl)methyl]-1H-pyrazole-4-carboxylic acid

[0676] ##STR00640##

Step 1: 6-Fluoro-3-formyl-2-methylbenzonitrile

[0677] .sup.iPrMgCl*LiCl (Turbo Grignard; 1.3 mol/L in THF, 3.8 mL) is added dropwise to 3-bromo-6-fluoro-2-methylbenzonitrile (1.0 g) in THF (25 mL) at −20° C. The mixture is warmed to 0° C. over a period of 1.3 h prior to the addition of another portion of .sup.iPrMgCl*LiCl (Turbo Grignard; 1.3 mol/L in THF, 1.0 mL). The cooling bath is removed, and the mixture is stirred for another 45 min. The mixture is cooled to −20° C., and DMF (0.8 mL) is added. After stirring for 50 min, the cooling bath is removed, and the reaction is quenched by adding aqueous NH.sub.4Cl solution at rt. The mixture is extracted with EtOAc (3×), and the combined extract is dried (Na.sub.2SO.sub.4) and concentrated. The residue is chromatographed on silica gel (cyclohexane/EtOAc 24:1.fwdarw.3:1) to give the title compound. LC (Method 2): t.sub.R=0.87 min.

Step 2: 6-{3-Azabicyclo[3.1.0]hexan-3-yl}-3-formyl-2-methylbenzonitrile

[0678] A mixture of 6-fluoro-3-formyl-2-methylbenzonitrile (515 mg), KHCO.sub.3 (0.79 g), 3-azabicyclo[3.1.0]hexane hydrochloride (453 mg), and DMSO (10 mL) is stirred at 70° C. for 1.3 h. After cooling to rt, water is added, and the mixture is stirred for 30 min. The precipitate is separated by filtration, washed with water (2×), and dried at 65° C. to give the title compound. LC (Method 1): t.sub.R=0.97 min; Mass spectrum (ESP): m/z=227 [M+H].sup.+.

Step 3: 6-{3-Azabicyclo[3.1.0]hexan-3-yl}-3-{[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3-cyano-2-methylphenyl)methoxy]methyl}-2-methylbenzonitrile

[0679] NaBH.sub.4 (0.21 g) is added portionwise to 6-{3-azabicyclo[3.1.0]hexan-3-yl}-3-formyl-2-methylbenzonitrile (0.63 g) in THF (10 mL) and MeOH (5 mL) at rt. The mixture is stirred for 1 h before aqueous HCl solution (1 mol/L) is added. The mixture is stirred for 1 h before it is neutralized with aqueous NaHCO.sub.3 solution. The mixture is extracted with EtOAc (2×), and the combined extract is dried (Na.sub.2SO.sub.4) and concentrated to afford a mixture of the title compound and 6-{3-azabicyclo[3.1.0]hexan-3-yl}-3-(methoxymethyl)-2-methylbenzonitrile that is used as is in the next reaction step (both components are competent starting materials for the next step).

[0680] LC (Method 1): t.sub.R=1.27 min; Mass spectrum (ESI.sup.+): m/z=439 [M+H].sup.+.

Step 4: Ethyl 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3-cyano-2-methylphenyl)methyl]-1H-pyrazole-4-carboxylate

[0681] The title compound is prepared from the mixture obtained in Step 3 of Intermediate 113, 6-{3-azabicyclo[3.1.0]hexan-3-yl}-3-{[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3-cyano-2-methylphenyl)methoxy]methyl}-2-methylbenzonitrile and 6-{3-azabicyclo[3.1.0]hexan-3-yl}-3-(methoxymethyl)-2-methylbenzonitrile, and ethyl 1H-pyrazole-4-carboxylate following a procedure analogous to that described in Step 3 of Intermediate 111.

[0682] LC (Method 1): t.sub.R=1.08 min; Mass spectrum (ESI.sup.+): m/z=351 [M+H].sup.+.

Step 5: 1-[(4-{3-Azabicyclo[3.1.0]hexan-3-yl}-3-cyano-2-methylphenyl)methyl]-1H-pyrazole-4-carboxylic acid

[0683] The title compound is prepared from ethyl 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3-cyano-2-methylphenyl)methyl]-1H-pyrazole-4-carboxylate following a procedure analogous to that described in Step 4 of Intermediate 111.

[0684] LC (Method 2): t.sub.R=0.96 min; Mass spectrum (ESI.sup.+): m/z=323 [M+H].sup.+.

Intermediate 114

1-[(4-{3-Azabicyclo[3.1.0]hexan-3-yl}-3-cyano-2-methylphenyl)methyl]-1H-imidazole-4-carboxylic acid

[0685] ##STR00641##

Step 1: Ethyl 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3-cyano-2-methylphenyl)methyl]-1H-imidazole-4-carboxylate

[0686] The title compound is prepared from the mixture obtained in Step 3 of Intermediate 113, 6-{3-azabicyclo[3.1.0]hexan-3-yl}-3-{[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3-cyano-2-methylphenyl)methoxy]methyl}-2-methylbenzonitrile and 6-{3-azabicyclo[3.1.0]hexan-3-yl}-3-(methoxymethyl)-2-methylbenzonitrile, and ethyl 1H-imidazole-4-carboxylate following a procedure analogous to that described in Step 3 of Intermediate 111.

[0687] LC (Method 1): t.sub.R=0.99 min; Mass spectrum (ESP): m/z=351 [M+H].sup.+.

Step 2: 1-[(4-{3-Azabicyclo[3.1.0]hexan-3-yl}-3-cyano-2-methylphenyl)methyl]-1H-imidazole-4-carboxylic acid

[0688] The title compound is prepared from ethyl 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3-cyano-2-methylphenyl)methyl]-1H-imidazole-4-carboxylate following a procedure analogous to that described in Step 4 of Intermediate 111.

[0689] LC (Method 2): t.sub.R=0.80 min; Mass spectrum (ESP): m/z=323 [M+H].sup.+.

Intermediate 115

1-[(4-{3-Azabicyclo[3.1.0]hexan-3-yl}-3-cyano-5-fluorophenyl)methyl]-1H-pyrazole-4-carboxylic acid

[0690] ##STR00642##

Step 1: 2,3-Difluoro-5-formylbenzonitrile

[0691] The title compound is prepared from 5-bromo-2,3-difluorobenzonitrile following a procedure analogous to that described in Step 1 of Intermediate 113. LC (Method 2): t.sub.R=0.84 min.

Step 2: 2-{3-Azabicyclo[3.1.0]hexan-3-yl}-3-fluoro-5-formylbenzonitrile

[0692] The title compound is prepared from 2,3-difluoro-5-formylbenzonitrile and 3-azabicyclo[3.1.0]hexane hydrochloride following a procedure analogous to that described in Step 1 of Intermediate 111.

[0693] LC (Method 2): t.sub.R=1.03 min; Mass spectrum (ESP): m/z=231 [M+H].sup.+.

Step 3: 2-{3-Azabicyclo[3.1.0]hexan-3-yl}-3-fluoro-5-(hydroxymethyl)benzonitrile

[0694] The title compound is prepared from 2-{3-azabicyclo[3.1.0]hexan-3-yl}-3-fluoro-5-formylbenzonitrile following a procedure analogous to that described in Step 2 of Intermediate 111.

[0695] LC (Method 2): t.sub.R=0.99 min; Mass spectrum (ESP): m/z=233 [M+H].sup.+.

Step 4: Ethyl 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3-cyano-5-fluorophenyl)methyl]-1H-pyrazole-4-carboxylate

[0696] The title compound is prepared from 2-{3-azabicyclo[3.1.0]hexan-3-yl}-3-fluoro-5-(hydroxymethyl)benzonitrile and ethyl 1H-pyrazole-4-carboxylate following a procedure analogous to that described in Step 3 of Intermediate 111.

[0697] LC (Method 2): t.sub.R=1.10 min; Mass spectrum (ESP): m/z=355 [M+H].sup.+.

Step 5: 1-[(4-{3-Azabicyclo[3.1.0]hexan-3-yl}-3-cyano-5-fluorophenyl)methyl]-1H-pyrazole-4-carboxylic acid

[0698] The title compound is prepared from ethyl 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3-cyano-5-fluorophenyl)methyl]-1H-pyrazole-4-carboxylate following a procedure analogous to that described in Step 4 of Intermediate 111.

[0699] LC (Method 2): t.sub.R=0.99 min; Mass spectrum (ESP): m/z=327 [M+H].sup.+.

Intermediate 116

1-[(4-{3-Azabicyclo[3.1.0]hexan-3-yl}-3-cyanophenyl)methyl]-1H-imidazole-4-carboxylic acid

[0700] ##STR00643##

Step 1: Ethyl 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3-cyanophenyl)methyl]-1H-imidazole-4-carboxylate

[0701] The title compound is prepared from 2-{3-azabicyclo[3.1.0]hexan-3-yl}-5-(hydroxymethyl)benzonitrile and ethyl 1H-imidazole-4-carboxylate following a procedure analogous to that described in Step 3 of Intermediate 111.

Step 2: 1-[(4-{3-Azabicyclo[3.1.0]hexan-3-yl}-3-cyanophenyl)methyl]-1H-imidazole-4-carboxylic acid

[0702] The title compound is prepared from ethyl 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3-cyanophenyl)methyl]-1H-imidazole-4-carboxylate following a procedure analogous to that described in Step 4 of Intermediate 111.

Intermediate 117

1-[(4-{3-Azabicyclo[3.1.0]hexan-3-yl}-3,5-dicyanophenyl)methyl]-1H-pyrazole-4-carboxylic acid

[0703] ##STR00644##

Step 1: 2-Fluoro-5-formylbenzene-1,3-dicarbonitrile

[0704] A mixture of 4-fluoro-3,5-diiodobenzaldehyde (2.00 g), copper(I) cyanide (1.05 g), and DMF (25 mL) is stirred at 120° C. for 24 h. After cooling to rt, water is added, and the resulting mixture is extracted with ethyl acetate (3×). The combined extract is dried (Na.sub.2SO.sub.4) and concentrated. The residue is chromatographed on silica gel (petroleum ether/EtOAc 95:51.fwdarw.70:30) to give the title compound.

Step 2: 2-{3-Azabicyclo[3.1.0]hexan-3-yl}-5-formylbenzene-1,3-dicarbonitrile

[0705] The title compound is prepared from 2-fluoro-5-formylbenzene-1,3-dicarbonitrile and 3-azabicyclo[3.1.0]hexane hydrochloride following a procedure analogous to that described in Step 1 of Intermediate 111.

[0706] LC (Method 2): t.sub.R=0.95 min; Mass spectrum (ESP): m/z=238 [M+H].sup.+.

Step 3: 2-{3-azabicyclo[3.1.0]hexan-3-yl}-5-(hydroxymethyl)benzene-1,3-dicarbonitrile

[0707] The title compound is prepared from 2-{3-azabicyclo[3.1.0]hexan-3-yl}-5-formylbenzene-1,3-dicarbonitrile following a procedure analogous to that described in Step 2 of Intermediate 111.

[0708] LC (Method 2): t.sub.R=0.92 min; Mass spectrum (ESI.sup.+): m/z=240 [M+H].sup.+.

Step 4: Ethyl 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3,5-dicyanophenyl)methyl]-1H-pyrazole-4-carboxylate

[0709] The title compound is prepared from 2-{3-azabicyclo[3.1.0]hexan-3-yl}-5-(hydroxymethyl)benzene-1,3-dicarbonitrile and ethyl 1H-pyrazole-4-carboxylate following a procedure analogous to that described in Step 3 of Intermediate 111.

[0710] LC (Method 2): t.sub.R=1.07 min; Mass spectrum (ESI.sup.+): m/z=362 [M+H].sup.+.

Step 5: 1-[(4-{3-Azabicyclo[3.1.0]hexan-3-yl}-3,5-dicyanophenyl)methyl]-1H-pyrazole-4-carboxylic acid

[0711] The title compound is prepared from ethyl 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3,5-dicyanophenyl)methyl]-1H-pyrazole-4-carboxylate following a procedure analogous to that described in Step 4 of Intermediate 111.

[0712] LC (Method 2): t.sub.R=0.92 min; Mass spectrum (ESI.sup.+): m/z=334 [M+H].sup.+.

Intermediate 118

1-[(4-{3-Azabicyclo[3.1.0]hexan-3-yl}-3-cyano-2-fluorophenyl)methyl]-1H-pyrazole-4-carboxylic acid

[0713] ##STR00645##

Step 1: 3-Bromo-2,6-difluorobenzonitrile

[0714] 3-Amino-2,6-difluorobenzonitrile (2.50 g) dissolved in ACN (45 mL) is added dropwise to a mixture of copper(II) bromide (4.49 g), tert-butyl nitrite (3.8 mL), and ACN (45 mL) stirred at 65° C. The mixture is stirred at 65° C. for 1 h and then cooled to rt. 20% Aqueous HCl solution is added, and the resulting mixture is extracted with diethyl ether. The combined extract is dried (Na.sub.2SO.sub.4) and concentrated. The residue is chromatographed on silica gel (cyclohexane/EtOAc 7:3) to give the title compound. LC (Method 2): t.sub.R=1.01 min.

Step 2: 6-{3-Azabicyclo[3.1.0]hexan-3-yl}-3-bromo-2-fluorobenzonitrile

[0715] The title compound is prepared from 3-bromo-2,6-difluorobenzonitrile and 3-azabicyclo[3.1.0]hexane hydrochloride following a procedure analogous to that described in Step 1 of Intermediate 111.

[0716] LC (Method 2): t.sub.R=1.17 min; Mass spectrum (ESI.sup.+): m/z=281/283 (Br) [M+H].sup.+.

Step 3: Methyl 4-{3-azabicyclo[3.1.0]hexan-3-yl}-3-cyano-2-fluorobenzoate

[0717] A mixture of 6-{3-azabicyclo[3.1.0]hexan-3-yl}-3-bromo-2-fluorobenzonitrile (500 mg), PdCl.sub.2(dppf) (72 mg), NEt.sub.3 (0.3 mL), and MeOH (6 mL) is stirred under an atmosphere of carbon monoxide (10 bar) at 80° C. overnight. After cooling to rt, the mixture is filtered, and the filtrate is concentrated. The residue is chromatographed on silica gel (cyclohexane/EtOAc 6:4) to give the title compound.

[0718] LC (Method 2): t.sub.R=1.05 min; Mass spectrum (ESI.sup.+): m/z=261 [M+H].sup.+.

Step 4: 6-{3-Azabicyclo[3.1.0]hexan-3-yl}-3-{[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3-cyano-2-fluorophenyl)methoxy]-methyl}-2-fluorobenzonitrile

[0719] Methyl 4-{3-azabicyclo[3.1.0]hexan-3-yl}-3-cyano-2-fluorobenzoate (0.42 g) dissolved in THF (5 mL) is added dropwise to LiAlH.sub.4 in THF (2.3 mol/L; 0.70 mL) at −50° C. The mixture is stirred while warming to −20° C. for 1.5 h and then quenched by the addition of aqueous HCl solution (1 mol/L). The resulting mixture is extracted with EtOAc (3×), and the combined extract is dried (Na.sub.2S04) and concentrated. The residue is chromatographed on silica gel (cyclohexane/EtOAc 1:0.fwdarw.1:1) to give the title compound. Depending on the workup procedure, 6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-fluoro-3-(hydroxymethyl)benzonitrile is also or exclusively obtained; the latter can be analogously used in the next reaction step. Mass spectrum (ESI.sup.+): m/z=447 [M+H].sup.+.

Step 5: Ethyl 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3-cyano-2-fluorophenyl)methyl]-1H-pyrazole-4-carboxylate

[0720] The title compound is prepared from 6-{3-azabicyclo[3.1.0]hexan-3-yl}-3-{[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3-cyano-2-fluorophenyl)methoxy]methyl}-2-fluorobenzonitrile and ethyl 1H-pyrazole-4-carboxylate following a procedure analogous to that described in Step 3 of Intermediate 111.

[0721] LC (Method 1): t.sub.R=1.06 min; Mass spectrum (ESI.sup.+): m/z=355 [M+H].sup.+.

Step 6: 1-[(4-{3-Azabicyclo[3.1.0]hexan-3-yl}-3-cyano-2-fluorophenyl)methyl]-1H-pyrazole-4-carboxylic acid

[0722] The title compound is prepared from ethyl 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3-cyano-2-fluorophenyl)methyl]-1H-pyrazole-4-carboxylate following a procedure analogous to that described in Step 4 of Intermediate 111.

[0723] LC (Method 2): t.sub.R=0.94 min; Mass spectrum (ESI.sup.+): m/z=349 [M+Na].sup.+.

Intermediate 119

1-[(4-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-methylphenyl)methyl]-1H-pyrazole-4-carboxylic acid

[0724] ##STR00646##

Step 1: 4-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-methylbenzaldehyde

[0725] The title compound is prepared from 4-fluoro-2-methylbenzaldehyde and 6,6-difluoro-3-azabicyclo[3.1.0]hexane following a procedure analogous to that described in Step 1 of Intermediate 111; K.sub.2CO.sub.3 instead of Hünig's base is used at 130° C. LC (Method 2): t.sub.R=1.01 min; Mass spectrum (ESP): m/z=238 [M+H].sup.+.

Step 2: (4-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-methylphenyl)methanol

[0726] The title compound is prepared from 4-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-methylbenzaldehyde following a procedure analogous to that described in Step 2 of Intermediate 111.

Step 3: Ethyl 1-[(4-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-methylphenyl)methyl]-1H-pyrazole-4-carboxylate

[0727] The title compound is prepared from (4-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-methylphenyl)methanol and ethyl 1H-pyrazole-4-carboxylate following a procedure analogous to that described in Step 3 of Intermediate 111.

[0728] LC (Method 2): t.sub.R=1.13 min; Mass spectrum (ESP): m/z=362 [M+H].sup.+.

Step 4: 1-[(4-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-methylphenyl)methyl]-1H-pyrazole-4-carboxylic acid

[0729] The title compound is prepared from ethyl 1-[(4-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-methylphenyl)methyl]-1H-pyrazole-4-carboxylate following a procedure analogous to that described in Step 4 of Intermediate 111.

[0730] LC (Method 2): t.sub.R=0.98 min; Mass spectrum (ESP): m/z=334 [M+H].sup.+.

Intermediate 120

1-[(4-{3-Azabicyclo[3.1.0]hexan-3-yl}-5-cyano-2-methylphenyl)methyl]-1H-pyrazole-4-carboxylic acid

[0731] ##STR00647##

Step 1: 5-Bromo-2-chloro-4-methylbenzonitrile

[0732] N-bromosuccinimide (3.50 g) and trifluoroacetic acid (25 mL) are added to 2-chloro-4-methylbenzonitrile (2.50 g) in concentrated sulfuric acid at rt. The mixture is stirred at rt for 24 h. The mixture is cooled to 0° C. and then slowly poured into an ice-cold solution of aqueous NaOH solution (4 mol/L; 125 mL). The precipitate is separated by filtration and purified by chromatography on silica gel (cyclohexane/EtOAc) to give the title compound.

[0733] LC (Method 1): t.sub.R=1.07 min.

Step 2: 2-Chloro-5-formyl-4-methylbenzonitrile

[0734] The title compound is prepared from 5-bromo-2-chloro-4-methylbenzonitrile following a procedure analogous to that described in Step 1 of Intermediate 113. LC (Method 1): t.sub.R=0.90 min; Mass spectrum (ESI): m/z=178 [M−H].sup.−.

Step 3: 2-{3-Azabicyclo[3.1.0]hexan-3-yl}-5-formyl-4-methylbenzonitrile

[0735] The title compound is prepared from 2-chloro-5-formyl-4-methylbenzonitrile and 3-azabicyclo[3.1.0]hexane hydrochloride following a procedure analogous to that described in Step 1 of Intermediate 111.

[0736] LC (Method 2): t.sub.R=1.02 min; Mass spectrum (ESP): m/z=227 [M+H].sup.+.

Step 4: 2-{3-Azabicyclo[3.1.0]hexan-3-yl}-5-(hydroxymethyl)-4-methylbenzonitrile

[0737] The title compound is prepared from 2-{3-azabicyclo[3.1.0]hexan-3-yl}-5-formyl-4-methylbenzonitrile following a procedure analogous to that described in Step 2 of Intermediate 111.

[0738] LC (Method 2): t.sub.R=0.96 min; Mass spectrum (ESP): m/z=229 [M+H].sup.+.

Step 5: Ethyl 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-5-cyano-2-methylphenyl)methyl]-1H-pyrazole-4-carboxylate

[0739] The title compound is prepared from 2-{3-azabicyclo[3.1.0]hexan-3-yl}-5-(hydroxymethyl)-4-methylbenzonitrile and ethyl 1H-pyrazole-4-carboxylate following a procedure analogous to that described in Step 3 of Intermediate 111.

[0740] LC (Method 1): t.sub.R=1.08 min; Mass spectrum (ESP): m/z=351 [M+H].sup.+.

Step 6: 1-[(4-{3-Azabicyclo[3.1.0]hexan-3-yl}-5-cyano-2-methylphenyl)methyl]-1H-pyrazole-4-carboxylic acid

[0741] The title compound is prepared from ethyl 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-5-cyano-2-methylphenyl)methyl]-1H-pyrazole-4-carboxylate following a procedure analogous to that described in Step 4 of Intermediate 111.

[0742] LC (Method 1): t.sub.R=0.68 min; Mass spectrum (ESI.sup.+): m/z=323 [M+H].sup.+.

Intermediate 121

1-[(4-{3-Azabicyclo[3.1.0]hexan-3-yl}-3-cyano-2-fluorophenyl)methyl]-1H-imidazole-4-carboxylic acid

[0743] ##STR00648##

Step 1: Ethyl 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3-cyano-2-fluorophenyl)methyl]-1H-imidazole-4-carboxylate

[0744] The title compound is prepared from a mixture of 6-{3-azabicyclo[3.1.0]hexan-3-yl}-3-{[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3-cyano-2-fluorophenyl)methoxy]methyl}-2-fluorobenzonitrile and 6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-fluoro-3-(hydroxymethyl)benzonitrile, obtained after workup in Step 4 of Intermediate 118, and ethyl 1H-imidazole-4-carboxylate following a procedure analogous to that described in Step 3 of Intermediate 111; the reaction is conducted at 140° C. in a microwave oven. LC (Method 1): t.sub.R=0.90 min; Mass spectrum (ESP): m/z=355 [M+H].sup.+.

Step 2: 1-[(4-{3-Azabicyclo[3.1.0]hexan-3-yl}-3-cyano-2-fluorophenyl)methyl]-1H-imidazole-4-carboxylic acid

[0745] The title compound is prepared from ethyl 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3-cyano-2-fluorophenyl)methyl]-1H-imidazole-4-carboxylate following a procedure analogous to that described in Step 4 of Intermediate 111.

[0746] LC (Method 2): t.sub.R=0.78 min; Mass spectrum (ESP): m/z=327 [M+H].sup.+.

Intermediate 122

1-[(4-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-chloro-3-cyanophenyl)methyl]-1H-pyrazole-4-carboxylic acid

[0747] ##STR00649##

Step 1: 3-Bromo-2,6-dichlorobenzonitrile

[0748] KBrO.sub.3 (7.28 g) is added in portions to 2,6-dichlorobenzonitrile (2.50 g) in concentrated sulfuric acid chilled in an ice bath. The mixture is warmed in the cooling bath to rt and then stirred at this temperature overnight. The mixture is poured onto ice, and saturated aqueous K.sub.2CO.sub.3 solution is added to neutralize the solution. The resulting mixture is extracted with DCM (3×), and the combined extract is dried (Na.sub.2SO.sub.4) and concentrated. The residue is chromatographed on silica gel (cyclohexane/EtOAc 1:0.fwdarw.7:3) to give the title compound.

[0749] LC (Method 2): t.sub.R=1.09 min.

Step 2: Methyl 2,4-dichloro-3-cyanobenzoate

[0750] The title compound is prepared from 3-bromo-2,6-dichlorobenzonitrile following a procedure analogous to that described in Step 3 of Intermediate 118; the reaction is conducted in a mixture of DMF and MeOH.

[0751] LC (Method 2): t.sub.R=1.00 min.

Step 3: Methyl 4-{3-azabicyclo[3.1.0]hexan-3-yl}-2-chloro-3-cyanobenzoate

[0752] The title compound is prepared from methyl 2,4-dichloro-3-cyanobenzoate and 3-azabicyclo[3.1.0]hexane hydrochloride following a procedure analogous to that described in Step 1 of Intermediate 111.

[0753] LC (Method 2): t.sub.R=1.07 min; Mass spectrum (ESP): m/z=277 [M+H].sup.+.

Step 4: 6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-chloro-3-(hydroxymethyl)benzonitrile

[0754] The title compound is prepared from methyl 4-{3-azabicyclo[3.1.0]hexan-3-yl}-2-chloro-3-cyanobenzoate following a procedure analogous to that described in Step 4 of Intermediate 118.

[0755] LC (Method 2): t.sub.R=0.96 min; Mass spectrum (ESP): m/z=249 [M+H].sup.+.

Step 5: Ethyl 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-2-chloro-3-cyano-6-methylphenyl)methyl]-1H-pyrazole-4-carboxylate

[0756] The title compound is prepared from 6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-chloro-3-(hydroxymethyl)benzonitrile and ethyl 1H-pyrazole-4-carboxylate following a procedure analogous to that described in Step 3 of Intermediate 111.

[0757] LC (Method 2): t.sub.R=1.10 min.

Step 6: 1-[(4-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-chloro-3-cyanophenyl)methyl]-1H-pyrazole-4-carboxylic acid

[0758] The title compound is prepared from ethyl 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-2-chloro-3-cyano-6-methylphenyl)methyl]-1H-pyrazole-4-carboxylate following a procedure analogous to that described in Step 4 of Intermediate 111. LC (Method 2): t.sub.R=0.96 min.

Intermediate 123

1-[(4-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-chloro-3-cyanophenyl)methyl]-1H-imidazole-4-carboxylic acid

[0759] ##STR00650##

Step 1: Ethyl 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-2-chloro-3-cyanophenyl)methyl]-1H-imidazole-4-carboxylate

[0760] The title compound is prepared from 6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-chloro-3-(hydroxymethyl)benzonitrile and ethyl 1H-imidazole-4-carboxylate following a procedure analogous to that described in Step 3 of Intermediate 111; the reaction is conducted at 100° C. LC (Method 1): t.sub.R=0.98 min; Mass spectrum (ESI.sup.+): m/z=371 [M+H].sup.+.

Step 2: 1-[(4-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-chloro-3-cyanophenyl)methyl]-1H-imidazole-4-carboxylic acid

[0761] The title compound is prepared from ethyl 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-2-chloro-3-cyanophenyl)methyl]-1H-imidazole-4-carboxylate following a procedure analogous to that described in Step 4 of Intermediate 111.

[0762] LC (Method 2): t.sub.R=0.80 min; Mass spectrum (ESP): m/z=343 [M+H].sup.+.

Intermediate 124

1-[(4-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-bromophenyl)methyl]-1H-imidazole-4-carboxylic acid

[0763] ##STR00651##

Step 1: 4-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-bromobenzaldehyde

[0764] The title compound is prepared from 2-bromo-4-fluorobenzaldehyde and 3-azabicyclo[3.1.0]hexane hydrochloride following a procedure analogous to that described in Step 1 of Intermediate 111; K.sub.2CO.sub.3 instead of Hünig's base and NMP instead of DMF are used at 120° C.

[0765] LC (Method 2): t.sub.R=1.08 min; Mass spectrum (ESP): m/z=266/268 (Br) [M+H].sup.+.

Step 2: (4-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-bromophenyl)methanol

[0766] The title compound is prepared from 4-{3-azabicyclo[3.1.0]hexan-3-yl}-2-bromobenzaldehyde following a procedure analogous to that described in Step 2 of Intermediate 111.

[0767] LC (Method 2): t.sub.R=1.01 min; Mass spectrum (ESP): m/z=268/270 (Br) [M+H].sup.+.

Step 3: Ethyl 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-2-bromophenyl)methyl]-1H-imidazole-4-carboxylate

[0768] The title compound is prepared from (4-{3-azabicyclo[3.1.0]hexan-3-yl}-2-bromophenyl)methanol and ethyl 1H-imidazole-4-carboxylate following a procedure analogous to that described in Step 3 of Intermediate 111.

[0769] LC (Method 2): t.sub.R=0.96 min; Mass spectrum (ESP): m/z=390/392 (Br) [M+H].sup.+.

Step 4: 1-[(4-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-bromophenyl)methyl]-1H-imidazole-4-carboxylic acid

[0770] The title compound is prepared from ethyl 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-2-bromophenyl)methyl]-1H-imidazole-4-carboxylate following a procedure analogous to that described in Step 4 of Intermediate 111.

[0771] LC (Method 2): t.sub.R=0.85 min.

Intermediate 125

1-[(4-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-methylphenyl)methyl]-1H-imidazole-4-carboxylic acid

[0772] ##STR00652##

Step 1: Ethyl 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-2-methylphenyl)methyl]-1H-imidazole-4-carboxylate

[0773] A flask charged with a stir bar, ethyl 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-2-bromophenyl)methyl]-1H-imidazole-4-carboxylate (100 mg), methylboronic acid (23 mg), Cs.sub.2CO.sub.3 (0.25 g), and 1,4-dioxane (1.5 mL) is flushed with Ar for 10 min. PdCl.sub.2(dppf) (21 mg) is added, the flask is sealed, and the mixture is stirred at 110° C. for 1.5 h. After cooling to rt, the mixture is diluted with MeOH and chromatographed (HPLC; ACN/water/ammonia) to give the title compound.

[0774] LC (Method 2): t.sub.R=0.91 min.

Step 2: 1-[(4-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-methylphenyl)methyl]-1H-imidazole-4-carboxylic acid

[0775] The title compound is prepared from ethyl 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-2-methylphenyl)methyl]-1H-imidazole-4-carboxylate following a procedure analogous to that described in Step 4 of Intermediate 111.

[0776] LC (Method 2): t.sub.R=0.81 min.

Intermediate 126

1-[(4-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-cyanophenyl)methyl]-1H-imidazole-4-carboxylic acid

[0777] ##STR00653##

Step 1: Ethyl 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-2-cyanophenyl)methyl]-1H-imidazole-4-carboxylate

[0778] A flask charged with a stir bar, ethyl 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-2-bromophenyl)methyl]-1H-imidazole-4-carboxylate (100 mg), Zn(CN).sub.2 (60 mg), zinc (8 mg), Pd.sub.2(dba).sub.3 (23 mg), and .sup.tBu.sub.3P*HBF.sub.4 (15 mg) is flushed with Ar for 10 min. NMP (1 mL) is added, the flask is sealed, and the mixture is stirred at 80° C. for 2 h. After cooling to rt, the mixture is diluted with DMF and chromatographed (HPLC; ACN/water/ammonia) to give the title compound.

[0779] LC (Method 2): t.sub.R=0.90 min; Mass spectrum (ESP): m/z=337 [M+H].sup.+.

Step 2: 1-[(4-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-cyanophenyl)methyl]-1H-imidazole-4-carboxylic acid

[0780] The title compound is prepared from ethyl 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-2-cyanophenyl)methyl]-1H-imidazole-4-carboxylate following a procedure analogous to that described in Step 4 of Intermediate 111.

[0781] LC (Method 2): t.sub.R=0.79 min; Mass spectrum (ESP): m/z=309 [M+H].sup.+.

Intermediate 127

1-[(4-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(hydroxymethyl)phenyl)methyl]-1H-imidazole-4-carboxylic acid

[0782] ##STR00654##

Step 1: Ethyl 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-2-ethenylphenyl)methyl]-1H-imidazole-4-carboxylate

[0783] A flask charged with a stir bar, ethyl 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-2-bromophenyl)methyl]-1H-imidazole-4-carboxylate (500 mg), vinylboronic acid (0.25 mL), aq. Na.sub.2CO.sub.3 solution (1 mol/L; 3.2 mL), and 1,4-dioxane (9 mL) is flushed with Ar for 10 min. PdCl.sub.2(dppf) (53 mg) is added, the flask is sealed, and the mixture is stirred at 100° C. for 2.5 h. After cooling to rt, the mixture is diluted with brine, and the resulting mixture is extracted with EtOAc (3×). The combined extract is dried (Na.sub.2SO.sub.4) and concentrated. The residue is chromatographed on silica gel (cyclohexane/EtOAc 4:1.fwdarw.0:1) to give the title compound. LC (Method 2): t.sub.R=0.93 min.

Step 2: Ethyl 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-2-formylphenyl)methyl]-1H-imidazole-4-carboxylate

[0784] OsO.sub.4 (4% in water; 0.14 mL) is added to a mixture of ethyl 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-2-ethenylphenyl)methyl]-1H-imidazole-4-carboxylate (300 mg), water (4 mL), and 1,4-dioxane (4 mL) at room temperature. After stirring the mixture for 10 min, NaIO.sub.4 (0.57 g) is added. The mixture is stirred for 2.5 h, and then ethyl acetate/methanol (9:1; 20 mL) and water (20 mL) are added. The mixture is extracted with ethyl acetate (3×), and the combined extract is dried (Na.sub.2SO.sub.4) and concentrated. The residue is chromatographed on silica gel (cyclohexane/ethyl acetate 70:30->0:1) to give the title compound.

[0785] LC (Method 1): t.sub.R=0.99 min; Mass spectrum (ESI.sup.+): m/z=340 [M+H].sup.+.

Step 3: Ethyl 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-2-(hydroxymethyl)phenyl)methyl]-1H-imidazole-4-carboxylate

[0786] The title compound is prepared from ethyl 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-2-formylphenyl)methyl]-1H-imidazole-4-carboxylate following a procedure analogous to that described in Step 2 of Intermediate 111.

[0787] LC (Method 1): t.sub.R=0.81 min.

Step 4: 1-[(4-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(hydroxymethyl)phenyl)methyl]-1H-imidazole-4-carboxylic acid

[0788] The title compound is prepared from ethyl 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-2-(hydroxymethyl)phenyl)methyl]-1H-imidazole-4-carboxylate following a procedure analogous to that described in Step 4 of Intermediate 111.

[0789] LC (Method 2): t.sub.R=0.72 min.

Intermediate 128

1-[(2-Cyano-4-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}phenyl)methyl]-1H-pyrazole-4-carboxylic acid

[0790] ##STR00655##

Step 1: Ethyl 1-[(2-cyano-4-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}phenyl)methyl]-1H-pyrazole-4-carboxylate

[0791] The title compound is prepared from ethyl 1-[(2-bromo-4-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}phenyl)methyl]-1H-pyrazole-4-carboxylate following a procedure analogous to that described in Step 1 of Intermediate 126.

[0792] LC (Method 1): t.sub.R=1.03 min.

Step 2: 1-[(2-Cyano-4-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}phenyl)methyl]-1H-pyrazole-4-carboxylic acid

[0793] The title compound is prepared from ethyl 1-[(2-cyano-4-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}phenyl)methyl]-1H-pyrazole-4-carboxylate following a procedure analogous to that described in Step 4 of Intermediate 111.

[0794] LC (Method 1): t.sub.R=0.63 min.

Intermediate 129

1-[(2-Bromo-4-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}phenyl)methyl]-1H-pyrazole-4-carboxylic acid

[0795] ##STR00656##

Step 1: 2-Bromo-4-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}benzaldehyde

[0796] The title compound is prepared from 2-bromo-4-fluorobenzaldehyde and 6,6-difluoro-3-azabicyclo[3.1.0]hexane hydrochloride following a procedure analogous to that described in Step 1 of Intermediate 111; K.sub.2CO.sub.3 instead of Hünig's base and NMP instead of DMF are used at 120° C.

[0797] LC (Method 2): t.sub.R=1.02 min; Mass spectrum (ESI.sup.+): m/z=302/304 (Br) [M+H].sup.+.

Step 2: (2-Bromo-4-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}phenyl)methanol

[0798] The title compound is prepared from 2-bromo-4-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}benzaldehyde following a procedure analogous to that described in Step 2 of Intermediate 111.

[0799] LC (Method 2): t.sub.R=0.96 min; Mass spectrum (ESI.sup.+): m/z=304/306 (Br) [M+H].sup.+.

Step 3: Ethyl 1-[(2-bromo-4-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}phenyl)methyl]-1H-pyrazole-4-carboxylate

[0800] The title compound is prepared from (2-bromo-4-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}phenyl)methanol and ethyl 1H-pyrazole-4-carboxylate following a procedure analogous to that described in Step 3 of Intermediate 111.

[0801] LC (Method 2): t.sub.R=1.09 min; Mass spectrum (ESI.sup.+): m/z=426/428 (Br) [M+H].sup.+.

Step 4: 1-[(2-Bromo-4-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}phenyl)methyl]-1H-pyrazole-4-carboxylic acid

[0802] The title compound is prepared from ethyl 1-[(2-bromo-4-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}phenyl)methyl]-1H-pyrazole-4-carboxylate following a procedure analogous to that described in Step 4 of Intermediate 111.

[0803] LC (Method 1): t.sub.R=0.68 min.

Intermediate 130

1-[(2-Cyano-4-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}phenyl)methyl]-1H-imidazole-4-carboxylic acid

[0804] ##STR00657##

Step 1: Ethyl 1-[(2-bromo-4-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}phenyl)methyl]-1H-imidazole-4-carboxylate

[0805] The title compound is prepared from (2-bromo-4-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}phenyl)methanol and ethyl 1H-imidazole-4-carboxylate following a procedure analogous to that described in Step 3 of Intermediate 111.

[0806] LC (Method 2): t.sub.R=0.91 min.

Step 2: Ethyl 1-[(2-cyano-4-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}phenyl)methyl]-1H-imidazole-4-carboxylate

[0807] The title compound is prepared from ethyl 1-[(2-bromo-4-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}phenyl)methyl]-1H-imidazole-4-carboxylate and zinc(II) cyanide following a procedure analogous to that described in Step 1 of Intermediate 126. LC (Method 1): t.sub.R=0.95 min; Mass spectrum (ESP): m/z=373 [M+H].sup.+.

Step 3: 1-[(2-Cyano-4-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}phenyl)methyl]-1H-imidazole-4-carboxylic acid

[0808] The title compound is prepared from ethyl 1-[(2-cyano-4-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}phenyl)methyl]-1H-imidazole-4-carboxylate following a procedure analogous to that described in Step 4 of Intermediate 111.

[0809] LC (Method 1): t.sub.R=0.64 min; Mass spectrum (ESI.sup.+): m/z=345 [M+H].sup.+.

Intermediate 131

1-[(2-Chloro-4-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}phenyl)methyl]-1H-imidazole-4-carboxylic acid

[0810] ##STR00658##

Step 1: Ethyl 1-[(2-chloro-4-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}phenyl)methyl]-1H-imidazole-4-carboxylate

[0811] A mixture of ethyl 1-[(2-bromo-4-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}phenyl)methyl]-1H-imidazole-4-carboxylate (200 mg), copper(I) chloride (92 mg), and NMP is stirred at 160° C. for 2.5 h. After cooling to rt, the mixture is diluted with water and extracted with EtOAc (3×). The combined extract is dried (Na.sub.2SO.sub.4) and concentrated. The residue is chromatographed (HPLC; ACN/water/ammonia) to give the title compound.

[0812] LC (Method 2): t.sub.R=0.92 min; Mass spectrum (ESP): m/z=382 [M+H].sup.+.

Step 2: 1-[(2-Chloro-4-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}phenyl)methyl]-1H-imidazole-4-carboxylic acid

[0813] The title compound is prepared from ethyl 1-[(2-chloro-4-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}phenyl)methyl]-1H-imidazole-4-carboxylate following a procedure analogous to that described in Step 4 of Intermediate 111.

[0814] LC (Method 1): t.sub.R=0.67 min; Mass spectrum (ESI): m/z=352 [M−H].sup.−.

Intermediate 132

1-[(4-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-fluorophenyl)methyl]-1H-imidazole-4-carboxylic acid

[0815] ##STR00659##

Step 1:4-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-fluorobenzaldehyde

[0816] The title compound is prepared from 2,4-difluorobenzaldehyde and 6,6-difluoro-3-azabicyclo[3.1.0]hexane hydrochloride following a procedure analogous to that described in Step 1 of Intermediate 111; K.sub.2CO.sub.3 instead of Hünig's base and NMP instead of DMF are used. Mass spectrum (ESI.sup.+): m/z=242 [M+H].sup.+.

Step 2: (4-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-fluorophenyl)methanol

[0817] The title compound is prepared from 4-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-fluorobenzaldehyde following a procedure analogous to that described in Step 2 of Intermediate 111.

[0818] LC (Method 2): t.sub.R=0.93 min; Mass spectrum (ESI.sup.+): m/z=244 [M+H].sup.+.

Step 3: Ethyl 1-[(4-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-fluorophenyl)methyl]-1H-imidazole-4-carboxylate

[0819] The title compound is prepared from (4-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-fluorophenyl)methanol and ethyl 1H-imidazole-4-carboxylate following a procedure analogous to that described in Step 3 of Intermediate 111.

Step 4: 1-[(4-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-fluorophenyl)methyl]-1H-imidazole-4-carboxylic acid

[0820] The title compound is prepared from ethyl 1-[(4-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-fluorophenyl)methyl]-1H-imidazole-4-carboxylate following a procedure analogous to that described in Step 4 of Intermediate 111.

[0821] LC (Method 2): t.sub.R=0.79 min.

Intermediate 133

1-[(4-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-fluorophenyl)methyl]-1H-pyrazole-4-carboxylic acid

[0822] ##STR00660##

Step 1: Ethyl 1-[(4-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-fluorophenyl)methyl]-1H-pyrazole-4-carboxylate

[0823] The title compound is prepared from (4-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-fluorophenyl)methanol and ethyl 1H-pyrazole-4-carboxylate following a procedure analogous to that described in Step 3 of Intermediate 111.

[0824] LC (Method 2): t.sub.R=1.10 min; Mass spectrum (ESI.sup.+): m/z=366 [M+H].sup.+.

Step 2: 1-[(4-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-fluorophenyl)methyl]-1H-pyrazole-4-carboxylic acid

[0825] The title compound is prepared from ethyl 1-[(4-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-fluorophenyl)methyl]-1H-pyrazole-4-carboxylate following a procedure analogous to that described in Step 4 of Intermediate 111.

SYNTHESIS OF EXAMPLES

Example 1

1-[(2-Chloro-6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]-1H-1,2,3-triazole-4-carboxamide

[0826] ##STR00661##

[0827] A mixture of 1-[(2-chloro-6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}pyridin-3-yl)methyl]-1H-1,2,3-triazole-4-carboxylic acid (46 mg), DIPEA (111 μL) and O-(7-azabenzotriazol-1-yl)-N,N,N′,N′-tetramethyluronium-hexafluorophosphat (HATU, 57 mg) in DMF (1 mL) is stirred for 5 min. (4R)-1-Methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-amine dihydrochloride (33 mg) is added and the mixture is stirred for 1 h. The mixture is purified by HPLC on reversed phase (ACN, water) to give the title compound.

[0828] LC (Method 1): t.sub.R=0.91 min; Mass spectrum (ESP): m/z=475 [M+H].sup.+.

[0829] Examples 2 to 213 are prepared in analogy to example 1:

TABLE-US-00180 Mass spectrum (ESI+): Intermediate Structure t.sub.R m/z [M+H].sup.+ LC Method 2 [00662] embedded image 0.64 484 Method 2 3 [00663] 0.62 404 Method 2 4 [00664] 0.95 455 Method 1 5 [00665] 0.78 439 Method 2 6 [00666] 0.80 483 Method 2 7 [00667] 0.73 458 Method 2 8 [00668] 0.64 405 Method 2 9 [00669] 0.62 407 Method 2 10 [00670] 0.77 423 Method 2 11 [00671] 0.73 422 Method 2 12 [00672] 0.77 422 Method 2 13 [00673] embedded image 0.79 482 Method 2 14 [00674] 0.93 519 Method 1 15 [00675] 0.92 518 Method 1 16 [00676] 0.76 482 Method 2 17 [00677] 0.78 423 Method 2 18 [00678] 0.77 458 Method 2 19 [00679] 0.64 404 Method 2 20 [00680] 0.94 433 Method 1 21 [00681] 0.91 438 Method 1 22 [00682] 0.96 432 Method 1 23 [00683] embedded image 0.55 418 Method 2 24 [00684] 0.89 454 Method 1 25 [00685] 0.77 459 Method 2 26 [00686] 0.59 405 Method 2 27 [00687] 0.90 455 Method 1 28 [00688] 0.92 518 Method 1 29 [00689] 0.74 455 Method 2 30 [00690] 0.82 455 Method 2 31 [00691] 0.95 447 Method 1 32 [00692] 0.80 439 Method 2 33 [00693] embedded image 0.58 441 Method 2 34 [00694] 0.81 483 Method 2 35 [00695] 0.75 429 Method 2 36 [00696] 0.64 468 Method 2 37 [00697] 0.93 432 Method 1 38 [00698] 0.84 488 Method 2 39 [00699] 0.77 482 Method 2 40 [00700] 0.81 482 Method 2 41 [00701] 0.82 440 Method 1 42 [00702] 0.76 469 Method 2 43 [00703] embedded image 0.95 454 Method 1 44 [00704] 0.79 474 Method 2 45 [00705] 0.93 490 Method 1 46 [00706] 0.92 490 Method 1 47 [00707] 0.93 454 Method 1 48 [00708] 0.79 455 Method 2 49 [00709] 0.90 448 Method 1 50 [00710] 0.84 471 Method 1 51 [00711] 0.92 432 Method 1 52 [00712] 0.78 454 Method 2 53 [00713] embedded image 0.62 440 Method 2 54 [00714] 0.67 462 Method 1 55 [00715] 0.90 485 Method 1 56 [00716] 0.78 438 Method 2 57 [00717] 0.91 474 Method 1 58 [00718] 0.88 488 Method 2 59 [00719] 0.90 462 Method 1 60 [00720] 0.55 405 Method 2 61 [00721] 0.59 435 Method 2 62 [00722] 0.64 435 Method 2 63 [00723] embedded image .058 440 Method 4 64 [00724] 0.95 454 Method 1 65 [00725] 1.00 471 Method 1 66 [00726] 0.98 482 Method 1 67 [00727] 1.02 494 Method 1 68 [00728] 0.87 419 Method 1 69 [00729] 0.98 482 Method 1 70 [00730] 0.97 443 Method 1 71 [00731] 0.79 405 Method 1 72 [00732] 0.87 404 Method 1 73 [00733] embedded image 0.86 404 Method 1 74 [00734] 0.88 404 Method 1 75 [00735] 0.60 432 Method 2 76 [00736] 0.60 468 Method 2 77 [00737] 0.63 448 Method 5 78 [00738] 0.62 448 Method 5 79 [00739] 0.58 434 Method 5 80 [00740] 0.58 440 Method 5 81 [00741] 0.89 443 Method 1 82 [00742] 0.89 448 Method 1 83 [00743] embedded image 0.89 448 Method 1 84 [00744] 0.61 462 Method 2 85 [00745] 0.85 448 Method 1 86 [00746] 0.85 434 Method 1 87 [00747] 0.52 434 Method 5 88 [00748] 0.89 448 Method 1 89 [00749] 0.69 435 Method 2 90 [00750] 0.62 454 Method 2 91 [00751] 0.86 435 Method 1 92 [00752] 0.93 449 Method 1 93 [00753] embedded image 0.60 470 Method 2 94 [00754] 0.76 456 Method 2 95 [00755] 0.64 484 Method 2 96 [00756] 0.59 470 Method 2 97 [00757] 0.73 470 Method 5 98 [00758] 0.88 434 Method 1 99 [00759] 0.89 438 Method 1 100 [00760] 0.65 460 Method 2 101 [00761] 0.99 433 Method 1 102 [00762] 0.83 484 Method 2 103 [00763] embedded image 0.88 479 Method 1 104 [00764] 0.78 422 Method 2 105 [00765] 0.87 479 Method 1 106 [00766] 0.87 418 Method 1 107 [00767] 0.88 484 Method 1 108 [00768] 0.74 422 Method 2 109 [00769] 0.96 469 Method 1 110 [00770] 0.62 446 Method 2 111 [00771] 0.62 468 Method 2 112 [00772] 0.64 468 Method 2 113 [00773] embedded image 0.63 418 Method 4 114 [00774] 0.75 429 Method 2 115 [00775] 0.93 472 Method 1 116 [00776] 0.76 443 Method 2 117 [00777] 0.66 444 Method 2 118 [00778] 0.74 443 Method 2 119 [00779] 0.81 486 Method 5 120 [00780] 0.89 438 Method 1 121 [00781] 0.93 472 Method 1 122 [00782] 0.77 437 Method 2 123 [00783] embedded image 0.94 462 Method 1 124 [00784] 0.77 443 Method 2 125 [00785] 0.97 476 Method 1 126 [00786] 0.93 498 Method 1 127 [00787] 0.92 438 Method 1 128 [00788] 0.93 443 Method 1 129 [00789] 0.97 432 Method 1 130 [00790] 0.97 432 Method 1 131 [00791] 0.59 404 Method 5 132 [00792] 0.67 429 Method 5 133 [00793] embedded image 0.89 418 Method 1 134 [00794] 0.80 472 Method 5 135 [00795] 0.61 405 Method 5 136 [00796] 0.69 455 Method 2 137 [00797] 0.86 420 Method 1 138 [00798] 0.64 441 Method 2 139 [00799] 0.60 439 Method 2 140 [00800] 0.60 439 Method 2 141 [00801] 0.62 453 Method 2 142 [00802] 0.63 453 Method 2 143 [00803] embedded image 1.02 446 Method 1 144 [00804] 1.01 455 Method 1 145 [00805] 0.98 491 Method 1 146 [00806] 1.03 433 Method 1 147 [00807] 0.88 471 Method 1 148 [00808] 0.98 439 Method 1 149 [00809] 0.91 435 Method 1 150 [00810] 0.96 475 Method 1 151 [00811] 0.88 405 Method 1 152 [00812] 0.94 455 Method 1 153 [00813] embedded image 1.06 469 Method 1 154 [00814] 0.91 419 Method 1 155 [00815] 0.89 455 Method 1 156 [00816] 0.97 433 Method 1 157 [00817] 0.95 439 Method 1 158 [00818] 0.78 433 Method 5 159 [00819] 0.82 473 Method 2 160 [00820] 1.07 510 Method 1 161 [00821] 1.03 496 Method 1 162 [00822] 1.05 460 Method 1 163 [00823] embedded image 0.97 482 Method 1 164 [00824] 0.98 446 Method 1 165 [00825] 0.96 432 Method 1 166 [00826] 0.66 446 Method 2 167 [00827] 0.94 468 Method 1 168 [00828] 0.96 432 Method 1 169 [00829] 0.62 418 Method 5 170 [00830] 0.62 418 Method 5 171 [00831] 0.59 419 Method 5 172 [00832] 0.55 419 Method 5 173 [00833] embedded image 0.71 469 Method 5 174 [00834] 0.64 470 Method 5 175 [00835] 0.63 506 Method 5 176 [00836] 0.66 474 Method 5 177 [00837] 0.59 419 Method 5 178 [00838] 0.62 456 Method 5 179 [00839] 0.92 498 Method 1 180 [00840] 0.90 448 Method 1 181 [00841] 0.63 418 Method 2 182 [00842] 0.63 418 Method 2 183 [00843] embedded image 1.08 448 Method 1 184 [00844] 0.69 484 Method 2 185 [00845] 0.65 486 Method 2 186 [00846] 0.67 468 Method 2 187 [00847] 0.67 470 Method 2 188 [00848] 0.66 471 Method 2 189 [00849] 0.66 454 Method 2 190 [00850] 0.80 428 Method 2 191 [00851] 0.98 439 Method 5 192 [00852] 0.98 439 Method 1 193 [00853] embedded image 0.92 442 Method 1 194 [00854] 0.92 442 Method 1 195 [00855] 0.83 446 Method 2 196 [00856] 0.78 428 Method 2 197 [00857] 0.79 453 Method 2 198 [00858] 0.59 446 Method 5 199 [00859] 0.84 453 Method 2 200 [00860] 0.72 442 Method 5 201 [00861] 0.67 446 Method 4 202 [00862] 0.83 462 Method 2 203 [00863] embedded image 0.54 462 Method 5 204 [00864] 0.82 481/483 (Br) Method 2 205 [00865] 0.78 417 Method 2 206 [00866] 0.69 428 Method 1 207 [00867] 0.87 433 Method 1 208 [00868] 0.90 464 Method 1 209 [00869] 0.95 517/519 (Br) Method 1 210 [00870] 0.65 464 Method 5 211 [00871] 0.74 473 Method 5 212 [00872] embedded image 0.70 457 Method 4 213 [00873] 0.81 457 Method 2

TABLE-US-00181 Example Name Name of Starting Material 2 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 1-[(6-{6,6-Difluoro-3- (methoxymethyl)pyridin-3-yl)methyl]-N-[(4R)-1-methyl- azabicyclo[3.1.0]hexan-3-yl}-2- 1H,4H,5H,6H-cyclopenta[d]-imidazol-4-yl]-1H-pyrazole-4- (methoxymethyl)pyridin-3-yl)methyl]- carboxamide 1H-pyrazole-4-carboxylic acid 3 1-[(5-{3-Azabicyclo[3.1.0]hexan-3-yl}pyridin-2-yl)methyl]- 1-[(5-{3-Azabicyclo[3.1.0]hexan-3- N-[(4R)-1-methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4- yl}pyridin-2-yl)methyl]-1H-imidazole-4- yl]-1H-imidazole-4-carboxamide carboxylic acid 4 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2-(difluoromethyl)- 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclo- (difluoromethyl)pyridin-3-yl)methyl]-1H- penta[d]imidazol-4-yl]-1H-1,2,3-triazole-4-carboxamide 1,2,3-triazole-4-carboxylic acid 5 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2-chloropyridin-3- 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclo- chloropyridin-3-yl)methyl]-1H-1,2,3- penta[d]imidazol-4-yl]-1H-1,2,3-triazole-4-carboxamide triazole-4-carboxylic acid 6 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2-bromopyridin-3- 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclo- bromopyridin-3-yl)methyl]-1H-1,2,3- penta[d]imidazol-4-yl]-1H-1,2,3-triazole-4-carboxamide triazole-4-carboxylic acid 7 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 1-[(6-{6,6-Difluoro-3-azabicyclo- fluoropyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- [3.1.0]hexan-3-yl}-2-fluoropyridin-3-yl)- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide methyl]-1H-imidazole-4-carboxylic acid 8 1-[(5-{3-Azabicyclo[3.1.0]hexan-3-yl}pyridin-2-yl)methyl]- 1-[(5-{3-Azabicyclo[3.1.0]hexan-3- N-[(4R)-1-methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4- yl}pyridin-2-yl)methyl]-1H-1,2,3- yl]-1H-1,2,3-triazole-4-carboxamide triazole-4-carboxylic acid 9 1-[(5-{3-Azabicyclo[3.1.0]hexan-3-yl}pyridin-2-yl)methyl]- 1-[(5-{3-Azabicyclo[3.1.0]hexan-3- N-[(4R)-1-methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4- yl}pyridin-2-yl)methyl]-1H-pyrazole-4- yl]-1H-pyrazole-4-carboxamide carboxylic acid 10 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2-fluoropyridin-3- 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclo- fluoropyridin-3-yl)methyl]-1H-1,2,3- penta[d]imidazol-4-yl]-1H-1,2,3-triazole-4-carboxamide triazole-4-carboxylic acid 11 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2-fluoropyridin-3- 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- fluoropyridin-3-yl)methyl]-1H-imidazole- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide 4-carboxylic acid 12 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2-fluoropyridin-3- 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- fluoropyridin-3-yl)methyl]-1H-pyrazole- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide 4-carboxylic acid 13 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2-bromopyridin-3- 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- bromopyridin-3-yl)methyl]-1H-pyrazole- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide 4-carboxylic acid 14 1-[(2-Bromo-6-{6,6-difluoro-3-azabicyclo-[3.1.0]hexan-3- 1-[(2-Bromo-6-{6,6-difluoro-3- yl}pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- cyclopenta[d]imidazol-4-yl]-1H-1,2,3-triazole-4- yl)methyl]-1H-1,2,3-triazole-4- carboxamide carboxylic acid 15 1-[(2-Bromo-6-{6,6-difluoro-3-azabicyclo-[3.1.0]hexan-3- 1-[(2-Bromo-6-{6,6-difluoro-3-aza- yl}pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- bicyclo[3.1.0]hexan-3-yl}pyridin-3-yl)- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide methyl]-1H-pyrazole-4-carboxylic acid 16 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2-bromopyridin-3- 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- bromopyridin-3-yl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide imidazole-4-carboxylic acid 17 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-fluoropyridin-3- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclo- fluoropyridin-3-yl)methyl]-1H-1,2,3- penta[d]imidazol-4-yl]-1H-1,2,3-triazole-4-carboxamide triazole-4-carboxylic acid 18 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 1-[(6-{6,6-Difluoro-3-azabicyclo- fluoropyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- [3.1.0]hexan-3-yl}-2-fluoropyridin-3-yl)- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide methyl]-1H-pyrazole-4-carboxylic acid 19 1-[(5-{5-Azaspiro[2.3]hexan-5-yl}pyridin-2-yl)-methyl]-N- 1-[(5-{5-Azaspiro[2.3]hexan-5- [(4R)-1-methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]- yl}pyridin-2-yl)methyl]-1H-pyrazole-4- 1H-pyrazole-4-carboxamide carboxylic acid 20 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2-ethylpyridin-3- 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclo- ethylpyridin-3-yl)methyl]-1H-1,2,3- penta[d]imidazol-4-yl]-1H-1,2,3-triazole-4-carboxamide triazole-4-carboxylic acid 21 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2-chloropyridin-3- 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- chloropyridin-3-yl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide imidazole-4-carboxylic acid 22 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-ethylpyridin-3- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- ethylpyridin-3-yl)methyl]-1H-pyrazole-4- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide carboxylic acid 23 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- methylpyridin-3-yl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide imidazole-4-carboxylic acid 24 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 1-[(6-{6,6-Difluoro-3-azabicyclo- methylpyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- [3.1.0]hexan-3-yl}-2-methylpyridin-3-yl)- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide methyl]-1H-imidazole-4-carboxylic acid 25 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 1-[(6-{6,6-Difluoro-3- fluoropyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- azabicyclo[3.1.0]hexan-3-yl}-2- cyclopenta[d]imidazol-4-yl]-1H-1,2,3-triazole-4- fluoropyridin-3-yl)methyl]-1H-1,2,3- carboxamide triazole-4-carboxylic acid 26 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}pyridin-3-yl)-methyl]-N- 1-[(6-{5-Azaspiro[2.3]hexan-5- [(4R)-1-methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]- yl}pyridin-3-yl)methyl]-1H-1,2,3- 1H-1,2,3-triazole-4-carboxamide triazole-4-carboxylic acid 27 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 1-[(6-{6,6-Difluoro-3- methylpyridin-3-yl)methyl]-N-[(4R)-1-methyl- azabicyclo[3.1.0]hexan-3-yl}-2- 1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]-1H-1,2,3- methylpyridin-3-yl)methyl]-1H-1,2,3- triazole-4-carboxamide triazole-4-carboxylic acid 28 1-[(2-Bromo-6-{6,6-difluoro-3-azabicyclo-[3.1.0]hexan-3- 1-[(2-Bromo-6-{6,6-difluoro-3-aza- yl}pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- bicyclo[3.1.0]hexan-3-yl}pyridin-3-yl)- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide methyl]-1H-imidazole-4-carboxylic acid 29 1-[(2-{3-Azabicyclo[3.1.0]hexan-3-yl}-4-(difluoromethyl)- 1-[(2-{3-Azabicyclo[3.1.0]hexan-3-yl}-4- pyrimidin-5-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- (difluoromethyl)pyrimidin-5-yl)methyl]- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide 1H-imidazole-4-carboxylic acid 30 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(difluoromethyl)- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclo- (difluoromethyl)pyridin-3-yl)methyl]-1H- penta[d]imidazol-4-yl]-1H-1,2,3-triazole-4-carboxamide 1,2,3-triazole-4-carboxylic acid 31 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-propylpyridin-3- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- propylpyridin-3-yl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide imidazole-4-carboxylic acid 32 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-chloropyridin-3- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclopenta- chloropyridin-3-yl)methyl]-1H-1,2,3- [d]imidazol-4-yl]-1H-1,2,3-triazole-4-carboxamide triazole-4-carboxylic acid 33 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}pyridin- 1-[(6-{6,6-Difluoro-3-azabicyclo- 3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclo- [3.10]hexan-3-yl}pyridin-3-yl)methyl]- penta[d]imidazol-4-yl]-1H-1,2,3-triazole-4-carboxamide 1H-1,2,3-triazole-4-carboxylic acid 34 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-bromopyridin-3- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclopenta- bromopyridin-3-yl)methyl]-1H-1,2,3- [d]imidazol-4-yl]-1H-1,2,3-triazole-4-carboxamide triazole-4-carboxylic acid 35 1-[(5-{3-Azabicyclo[3.1.0]hexan-3-yl}-6-cyanopyridin-2- 1-[(5-{3-Azabicyclo[3.1.0]hexan-3-yl}-6- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- cyanopyridin-2-yl)methyl]-1H-pyrazole- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide 4-carboxylic acid 36 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 1-[(6-{6,6-Difluoro-3-azabicyclo- ethylpyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- [3.1.0]hexan-3-yl}-2-ethylpyridin-3-yl)- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide methyl]-1H-pyrazole-4-carboxylic acid 37 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2-ethylpyridin-3- 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- ethylpyridin-3-yl)methyl]-1H-imidazole- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide 4-carboxylic acid 38 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(trifluoromethoxy)- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- (trifluoromethoxy)pyridin-3-yl)methyl]- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide 1H-imidazole-4-carboxylic acid 39 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-bromopyridin-3- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- bromopyridin-3-yl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide imidazole-4-carboxylic acid 40 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-bromopyridin-3- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- bromopyridin-3-yl)methyl]-1H-pyrazole- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide 4-carboxylic acid 41 1-[(5-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}pyridin- 1-[(5-{6,6-Difluoro-3-azabicyclo- 2-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- [3.10]hexan-3-yl}pyridin-2-yl)methyl]- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide 1H-pyrazole-4-carboxylic acid 42 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(1-cyanocyclopro- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- pyl)-pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- (1-cyanocyclopropyl)pyridin-3-yl)- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide methyl]-1H-pyrazole-4-carboxylic acid 43 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(difluoromethyl)- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- (difluoromethyl)pyridin-3-yl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide pyrazole-4-carboxylic acid 44 1-[(2-Chloro-6-{6,6-difluoro-3-azabicyclo-[3.1.0]hexan-3- 1-[(2-Chloro-6-{6,6-difluoro-3-aza- yl}pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- bicyclo[3.1.0]hexan-3-yl}pyridin-3-yl)- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide methyl]-1H-pyrazole-4-carboxylic acid 45 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 1-[(6-{6,6-Difluoro-3- (difluoromethyl)pyridin-3-yl)methyl]-N-[(4R)-1-methyl- azabicyclo[3.1.0]hexan-3-yl}-2- 1H,4H,5H,6H-cyclopenta-[d]imidazol-4-yl]-1H-imidazole- (difluoromethyl)pyridin-3-yl)methyl]-1H- 4-carboxamide imidazole-4-carboxylic acid 46 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 1-[(6-{6,6-Difluoro-3- (difluoromethyl)pyridin-3-yl)methyl]-N-[(4R)-1-methyl- azabicyclo[3.1.0]hexan-3-yl}-2- 1H,4H,5H,6H-cyclopenta-[d]imidazol-4-yl]-1H-pyrazole-4- (difluoromethyl)pyridin-3-yl)methyl]-1H- carboxamide pyrazole-4-carboxylic acid 47 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2-(difluoromethyl)- 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- (difluoromethyl)pyridin-3-yl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide imidazole-4-carboxylic acid 48 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(difluoromethyl)- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- (difluoromethyl)pyridin-3-yl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide pyrazole-4-carboxylic acid 49 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(methoxymethyl)- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- (methoxymethyl)pyridin-3-yl)methyl]- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide 1H-pyrazole-4-carboxylic acid 50 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 1-[(6-{6,6-Difluoro-3- (hydroxymethyl)pyridin-3-yl)methyl]-N-[(4R)-1-methyl- azabicyclo[3.1.0]hexan-3-yl}-2- 1H,4H,5H,6H-cyclopenta[d]-imidazol-4-yl]-1H-1,2,3- (hydroxymethyl)pyridin-3-yl)methyl]-1H- triazole-4-carboxamide 1,2,3-triazole-4-carboxylic acid 51 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2-ethylpyridin-3- 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- ethylpyridin-3-yl)methyl]-1H-pyrazole-4- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide carboxylic acid 52 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2-(difluoromethyl)- 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- (difluoromethyl)pyridin-3-yl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide pyrazole-4-carboxylic acid 53 1-[(5-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}pyridin- 1-[(5-{6,6-Difluoro-3-azabicyclo- 2-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- [3.10]hexan-3-yl}pyridin-2-yl)methyl]- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide 1H-imidazole-4-carboxylic acid 54 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(3-hydroxypropyl)- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- (3-hydroxypropyl)pyridin-3-yl)methyl]- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide 1H-pyrazole-4-carboxylic acid 55 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 1-[(6-{6,6-Difluoro-3- (methoxymethyl)pyridin-3-yl)methyl]-N-[(4R)-1-methyl- azabicyclo[3.1.0]hexan-3-yl}-2- 1H,4H,5H,6H-cyclopenta[d]-imidazol-4-yl]-1H-1,2,3- (methoxymethyl)pyridin-3-yl)methyl]- triazole-4-carboxamide 1H-1,2,3-triazole-4-carboxylic acid 56 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2-chloropyridin-3- 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- chloropyridin-3-yl)methyl]-1H-pyrazole- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide 4-carboxylic acid 57 1-[(2-Chloro-6-{6,6-difluoro-3-azabicyclo[3.1.0]-hexan-3- 1-[(2-Chloro-6-{6,6-difluoro-3-aza- yl}pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- bicyclo[3.1.0]hexan-3-yl}pyridin-3-yl)- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide methyl]-1H-imidazole-4-carboxylic acid 58 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(trifluoromethoxy)- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- (trifluoromethoxy)pyridin-3-yl)methyl]- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide 1H-pyrazole-4-carboxylic acid 59 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(1-hydroxypropan- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- 2-yl)pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- (1-hydroxypropan-2-yl)pyridin-3-yl)- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide methyl]-1H-pyrazole-4-carboxylic acid 60 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}pyridazin-3- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- yl}pyridazin-3-yl)methyl]-1H-pyrazole-4- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide carboxylic acid 61 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2-(hydroxymethyl)- 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclo- (hydroxymethyl)pyridin-3-yl)methyl]-1H- penta[d]imidazol-4-yl]-1H-1,2,3-triazole-4-carboxamide 1,2,3-triazole-4-carboxylic acid 62 1-[(2-{3-Azabicyclo[3.1.0]hexan-3-yl}-4-(hydroxymethyl)- 1-[(2-{3-Azabicyclo[3.1.0]hexan-3-yl}-4- pyrimidin-5-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- (hydroxymethyl)pyrimidin-5-yl)methyl]- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide 1H-pyrazole-4-carboxylic acid 63 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}pyridin- 1-[(6-{6,6-Difluoro-3-azabicyclo- 3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- [3.10]hexan-3-yl}pyridin-3-yl)methyl]- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide 1H-imidazole-4-carboxylic acid 64 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(difluoromethyl)- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- (difluoromethyl)pyridin-3-yl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide imidazole-4-carboxylic acid 65 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(1-cyano-1- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- methylethyl)pyridin-3-yl)methyl]-N-[(4R)-1-methyl- (1-cyano-1-methylethyl)pyridin-3-yl)- 1H,4H,5H,6H-cyclopenta[d]-imidazol-4-yl]-1H-pyrazole-4- methyl]-1H-pyrazole-4-carboxylic acid carboxamide 66 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 1-[(6-{6,6-Difluoro-3-azabicyclo- propylpyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- [3.1.0]hexan-3-yl}-2-propylpyridin-3-yl)- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide methyl]-1H-imidazole-4-carboxylic acid 67 1-[(2-Cydobutyl-6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan- 1-[(2-Cyclobutyl-6-{6,6-difluoro-3-aza- 3-yl}pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- bicyclo[3.1.0]hexan-3-yl}pyridin-3-yl)- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide methyl]-1H-pyrazole-4-carboxylic acid 68 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2-methylpyridin-3-yl)- 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclo- methylpyridin-3-yl)methyl]-1H-1,2,3- penta[d]imidazol-4-yl]-1H-1,2,3-triazole-4-carboxamide triazole-4-carboxylic acid 69 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]- propylpyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H - hexan-3-yl}-2-propylpyridin-3-yl)- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide methyl]-1H-pyrazole-4-carboxylic acid 70 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- yl)methyl]-3-cyano-N-[(4R)-1-methyl-1H,4H,5H,6H- methylpyridin-3-yl)methyl]-3-cyano-1H- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide pyrazole-4-carboxylic acid 71 1-[(5-{3-Azabicyclo[3.1.0]hexan-3-yl}pyrimidin-2- 1-[(5-{3-Azabicyclo[3.1.0]hexan-3- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- yl}pyrimidin-2-yl)methyl]-1H-pyrazole-4- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide carboxylic acid 72 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}pyridin-3-yl)methyl]- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3- N-[(4R)-1-methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4- yl}pyridin-3-yl)methyl]-1H-imidazole-4- yl]-1H-imidazole-4-carboxamide carboxylic acid 73 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}pyridin-3-yl)-methyl]-N- 1-[(6-{5-Azaspiro[2.3]hexan-5- [(4R)-1-methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]- yl}pyridin-3-yl)methyl]-1H-pyrazole-4- 1H-pyrazole-4-carboxamide carboxylic acid trifluoroacetate 74 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}pyridin-3-yl)-methyl]-N- 1-[(6-{5-Azaspiro[2.3]hexan-5- [(4R)-1-methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]- yl}pyridin-3-yl)methyl]-1H-imidazole-4- 1H-imidazole-4-carboxamide carboxylic acid trifluoroacetate 75 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-ethylpyridin-3- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- ethylpyridin-3-yl)methyl]-1H-imidazole- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide 4-carboxylic acid 76 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 1-[(6-{6,6-Difluoro-3-azabicyclo- ethylpyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- [3.1.0]hexan-3-yl}-2-ethylpyridin-3-yl)- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide methyl]-1H-imidazole-4-carboxylic acid 77 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2-(methoxymethyl)- 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- (methoxymethyl)pyridin-3-yl)methyl]- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide 1H-imidazole-4-carboxylic acid 78 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2-(methoxymethyl)- 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- (methoxymethyl)pyridin-3-yl)methyl]- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide 1H-pyrazole-4-carboxylic acid 79 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2-(hydroxymethyl)- 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- (hydroxymethyl)pyridin-3-yl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide pyrazole-4-carboxylic acid 80 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}pyridin- 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]- 3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- hexan-3-yl}pyridin-3-yl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide pyrazole-4-carboxylic acid trifluoroacetate 81 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(cyanomethyl)- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- (cyanomethyl)pyridin-3-yl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide pyrazole-4-carboxylic acid 82 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-[(1R)-1-hydroxy- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- ethyl]pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- [(1R)-1-hydroxyethyl]pyridin-3-yl)- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide methyl]-1H-pyrazole-4-carboxylic acid 83 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-[(1S)-1-hydroxy- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- ethyl]pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- [(1S)-1-hydroxyethyl]pyridin-3-yl)- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide methyl]-1H-pyrazole-4-carboxylic acid 84 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(2-hydroxypropan - 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- 2-yl)pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- (2-hydroxypropan-2-yl)pyridin-3-yl)- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide methyl]-1H-pyrazole-4-carboxylic acid 85 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(2-hydroxyethyl)- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- (2-hydroxyethyl)pyridin-3-yl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide pyrazole-4-carboxylic acid 86 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(hydroxymethyl)- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- (hydroxymethyl)pyridin-3-yl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide pyrazole-4-carboxylic acid 87 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2-(hydroxymethyl)- 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- (hydroxymethyl)pyridin-3-yl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide imidazole-4-carboxylic acid 88 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(1-hydroxyethyl)- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- (1-hydroxyethyl)pyridin-3-yl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide pyrazole-4-carboxylic acid 89 1-[(5-{3-Azabicyclo[3.1.0]hexan-3-yl}-3-(hydroxymethyl)- 1-[(5-{3-Azabicyclo[3.1.0]hexan-3-yl}-3- pyrazin-2-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- (hydroxymethyl)pyrazin-2-yl)methyl]- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide 1H-pyrazole-4-carboxylic acid 90 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]- methylpyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- hexan-3-yl}-2-methylpyridin-3-yl)- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide methyl]-1H-pyrazole-4-carboxylic acid 91 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(hydroxymethyl)- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclo- (hydroxymethyl)pyridin-3-yl)methyl]-1H- penta[d]imidazol-4-yl]-1H-1,2,3-triazole-4-carboxamide 1,2,3-triazole-4-carboxylic acid 92 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(methoxymethyl)- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclo- (methoxymethyl)pyridin-3-yl)methyl]- penta[d]imidazol-4-yl]-1H-1,2,3-triazole-4-carboxamide 1H-1,2,3-triazole-4-carboxylic acid 93 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 1-[(6-{6,6-Difluoro-3- (hydroxymethyl)pyridin-3-yl)methyl]-N-[(4R)-1-methyl- azabicyclo[3.1.0]hexan-3-yl}-2- 1H,4H,5H,6H-cyclopenta-[d]imidazol-4-yl]-1H-pyrazole-4- (hydroxymethyl)pyridin-3-yl)methyl]-1H- carboxamide pyrazole-4-carboxylic acid 94 1-[(5-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-3- 1-[(5-{6,6-Difluoro-3- methylpyrazin-2-yl)methyl]-N-[(4R)-1-methyl- azabicyclo[3.1.0]hexan-3-yl}-3- 1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]-1H-1,2,3- methylpyrazin-2-yl)methyl]-1H-1,2,3- triazole-4-carboxamide triazole-4-carboxylic acid 95 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 1-[(6-{6,6-Difluoro-3- (methoxymethyl)pyridin-3-yl)methyl]-N-[(4R)-1-methyl- azabicyclo[3.1.0]hexan-3-yl}-2- 1H,4H,5H,6H-cyclopenta-[d]imidazol-4-yl]-1H-imidazole- (methoxymethyl)pyridin-3-yl)methyl]- 4-carboxamide 1H-imidazole-4-carboxylic acid 96 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 1-[(6-{6,6-Difluoro-3- (hydroxymethyl)pyridin-3-yl)methyl]-N-[(4R)-1-methyl- azabicyclo[3.1.0]hexan-3-yl}-2- 1H,4H,5H,6H-cyclopenta-[d]imidazol-4-yl]-1H-imidazole- (hydroxymethyl)pyridin-3-yl)methyl]-1H- 4-carboxamide imidazole-4-carboxylic acid 97 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 1-[(6-{6,6-Difluoro-3- methoxypyridin-3-yl)methyl]-N-[(4R)-1-methyl- azabicyclo[3.1.0]hexan-3-yl}-2- 1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]-1H-imidazole-4- methoxypyridin-3-yl)methyl]-1H- carboxamide imidazole-4-carboxylic acid 98 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(hydroxymethyl)- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- (hydroxymethyl)pyridin-3-yl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide imidazole-4-carboxylic acid 99 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-4-chloropyridin-3- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-4- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- chloropyridin-3-yl)methyl]-1H-pyrazole- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide 4-carboxylic acid 100 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(2-methylpropyl)- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- (2-methylpropyl)pyridin-3-yl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide imidazole-4-carboxylic acid 101 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-ethylpyridin-3- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclo- ethylpyridin-3-yl)methyl]-1H-1,2,3- penta[d]imidazol-4-yl]-1H-1,2,3-triazole-4-carboxamide triazole-4-carboxylic acid 102 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]- ethoxypyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- hexan-3-yl}-2-ethoxypyridin-3-yl)- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide methyl]-1H-imidazole-4-carboxylic acid 103 1-[(5-Cyano-6-{6,6-difluoro-3-azabicyclo[3.1.0]-hexan-3- 1-[(5-Cyano-6-{6,6-difluoro-3- yl}-4-methylpyridin-3-yl)methyl]-N-[(4R)-1-methyl- azabicyclo[3.1.0]hexan-3-yl}-4- 1H,4H,5H,6H-cyclopenta-[d]imidazol-4-yl]-1H-pyrazole-4- methylpyridin-3-yl)methyl]-1H-pyrazole- carboxamide 4-carboxylic acid 104 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-fluoropyridin-3-yl)- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- fluoropyridin-3-yl)methyl]-1H-pyrazole- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide 4-carboxylic acid 105 1-[(5-Cyano-6-{6,6-difluoro-3-azabicyclo[3.1.0]-hexan-3- 1-[(5-Cyano-6-{6,6-difluoro-3- yl}-4-methylpyridin-3-yl)methyl]-N-[(4R)-1-methyl- azabicyclo[3.1.0]hexan-3-yl}-4- 1H,4H,5H,6H-cyclopenta-[d]imidazol-4-yl]-1H-imidazole- methylpyridin-3-yl)methyl]-1H- 4-carboxamide imidazole-4-carboxylic acid 106 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-4-methylpyridin-3- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-4- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- methylpyridin-3-yl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide imidazole-4-carboxylic acid 107 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]- methylpyridin-3-yl)methyl]-3-(hydroxymethyl)-N-[(4R)-1- hexan-3-yl}-2-methylpyridin-3- methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]-1H- yl)methyl]-3-(hydroxymethyl)-1H- pyrazole-4-carboxamide pyrazole-4-carboxylic acid 108 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-fluoropyridin-3- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- fluoropyridin-3-yl)methyl]-1H-imidazole- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide 4-carboxylic acid 109 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-ethyl- 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]- pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclo- hexan-3-yl}-2-ethylpyridin-3-yl)methyl]- penta[d]imidazol-4-yl]-1H-1,2,3-triazole-4-carboxamide 1H-1,2,3-triazole-4-carboxylic acid 110 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-propylpyridin-3- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- propylpyridin-3-yl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide imidazole-4-carboxylic acid 111 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2,4-di- 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]- methylpyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- hexan-3-yl}-2,4-dimethylpyridin-3-yl)- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide methyl]-1H-imidazole-4-carboxylic acid 112 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2,4-di- 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]- methylpyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H - hexan-3-yl}-2,4-dimethylpyridin-3-yl)- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide methyl]-1H-pyrazole-4-carboxylic acid 113 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-4-methylpyridin-3- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-4- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- methylpyridin-3-yl)methyl]-1H-pyrazole- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide 4-carboxylic acid 114 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-5-cyanopyridin-3- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-5- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- cyanopyridin-3-yl)methyl]-1H-pyrazole- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide 4-carboxylic acid 115 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-4-(trifluoromethyl)- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-4- pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- (trifluoromethyl)pyridin-3-yl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide pyrazole-4-carboxylic acid, lithium salt 116 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-5-cyano-4-methyl- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-5- pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- cyano-4-methylpyridin-3-yl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide pyrazole-4-carboxylic acid 117 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-cyclopropyl- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- cyclopropylpyridin-3-yl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide pyrazole-4-carboxylic acid 118 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-5-cyano-4-methyl- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-5- pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- cyano-4-methylpyridin-3-yl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide imidazole-4-carboxylic acid 119 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- methylpyridin-3-yl)methyl]-3- cyclopenta[d]imidazol-4-yl]-3-(trifluoromethyl)-1H- (trifluoromethyl)-1H-pyrazole-4- pyrazole-4-carboxamide carboxylic acid 120 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-4-chloropyridin-3- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-4- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- chloropyridin-3-yl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide imidazole-4-carboxylic acid 121 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-4-(trifluoromethyl)- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-4- pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- (trifluoromethyl)pyridin-3-yl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide imidazole-4-carboxylic acid 122 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-chloropyridin-3- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- chloropyridin-3-yl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide imidazole-4-carboxylic acid 123 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- yl)methyl]-3-(methoxymethyl)-N-[(4R)-1-methyl- methylpyridin-3-yl)methyl]-3-(methoxy- 1H,4H,5H,6H-cyclopenta-[d]imidazol-4-yl]-1H-pyrazole-4- methyl)-1H-pyrazole-4-carboxylic acid carboxamide 124 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-5-cyano-2-methyl- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-5- pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- cyano-2-methylpyridin-3-yl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide imidazole-4-carboxylic acid 125 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- yl)methyl]-3-(2-hydroxypropan-2-yl)-N-[(4R)-1-methyl- methylpyridin-3-yl)methyl]-3-(2- 1H,4H,5H,6H-cyclopenta-[d]imidazol-4-yl]-1H-pyrazole-4- hydroxypropan-2-yl)-1H-pyrazole-4- carboxamide carboxylic acid 126 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]- methylpyridin-3-yl)methyl]-3-(methoxymethyl)-N-[(4R)-1- hexan-3-yl}-2-methylpyridin-3- methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]-1H- yl)methyl]-3-(methoxymethyl)-1H- pyrazole-4-carboxamide pyrazole-4-carboxylic acid 127 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-chloropyridin-3- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- chloropyridin-3-yl)methyl]-1H-pyrazole- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide 4-carboxylic acid 128 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-5-cyano-2-methyl- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-5- pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- cyano-2-methylpyridin-3-yl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide pyrazole-4-carboxylic acid 129 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2,5-dimethylpyridin- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}- 3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- 2,5-dimethylpyridin-3-yl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide imidazole-4-carboxylic acid 130 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2,5-dimethylpyridin- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}- 3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- 2,5-dimethylpyridin-3-yl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide pyrazole-4-carboxylic acid 131 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}pyridin-3-yl)methyl]- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3- N-[(4R)-1-methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4- yl}pyridin-3-yl)methyl]-1H-pyrazole-4- yl]-1H-pyrazole-4-carboxamide carboxylic acid 132 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-cyanopyridin-3- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- cyanopyridin-3-yl)methyl]-1H-pyrazole- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide 4-carboxylic acid 133 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- methylpyridin-3-yl)methyl]-1H-pyrazole- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide 4-carboxylic acid 134 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(trifluoromethyl)- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- (trifluoromethyl)pyridin-3-yl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide pyrazole-4-carboxylic acid 135 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}pyridin-3-yl)methyl]- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3- N-[(4R)-1-methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4- yl}pyridin-3-yl)methyl]-1H-1,2,3- yl]-1H-1,2,3-triazole-4-carboxamide triazole-4-carboxylic acid 136 1-[(2-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-4- 1-[(2-{6,6-Difluoro-3-azabicyclo[3.1.0]- methylpyrimidin-5-yl)methyl]-N-[(4R)-1-methyl- hexan-3-yl}-4-methylpyrimidin-5-yl)- 1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]-1H-imidazole-4- methyl]-1H-imidazole-4-carboxylic acid carboxamide 137 1-[(2-{3-Azabicyclo[3.1.0]hexan-3-yl}-4-methylpyrimidin- 1-[(2-{3-Azabicyclo[3.1.0]hexan-3-yl}-4- 5-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclo- methylpyrimidin-5-yl)methyl]-1H-1,2,3- penta[d]imidazol-4-yl]-1H-1,2,3-triazole-4-carboxamide triazole-4-carboxylic acid 138 1-[(5-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}pyridin- 1-[(5-{6,6-Difluoro-3-azabicyclo[3.1.0]- 2-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclo- hexan-3-yl}pyridin-2-yl)methyl]-1H- penta[d]imidazol-4-yl]-1H-1,2,3-triazole-4-carboxamide 1,2,3-triazole-4-carboxylic acid 139 5-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}pyridin- 5-[(6-{6,6-Difluoro-3- 3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- cyclopenta[d]imidazol-4-yl]-1H-pyrrole-3-carboxamide yl)methyl]-1H-pyrrole-3-carboxylic acid 140 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}pyridin- 1-[(6-{6,6-Difluoro-3- 3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- cyclopenta[d]imidazol-4-yl]-1H-pyrrole-3-carboxamide yl)methyl]-1H-pyrrole-3-carboxylic acid 141 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]- methylpyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H - hexan-3-yl}-2-methylpyridin-3-yl)- cyclopenta[d]imidazol-4-yl]-1H-pyrrole-3-carboxamide methyl]-1H-pyrrole-3-carboxylic acid 142 5-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 5-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]- methylpyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H - hexan-3-yl}-2-methylpyridin-3- cyclopenta[d]imidazol-4-yl]-1H-pyrrole-3-carboxamide yl)methyl]-1H-pyrrole-3-carboxylic acid 143 1-({2-Ethyl-6-[(1R,5S,6R)-6-methyl-3-azabicyclo[3.1.0]- 1-({2-Ethyl-6-[(1R,5S,6R)-6-methyl-3- hexan-3-yl]pyridin-3-yl}methyl)-N-[(4R)-1-methyl- azabicyclo[3.1.0]hexan-3-yl]pyridin-3- 1H,4H,5H,6H-cyclopenta-[d]imidazol-4-yl]-1H-imidazole- yl}methyl)-1H-imidazole-4-carboxylic 4-carboxamide acid 144 3-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(difluoromethyl)- 3-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- (difluoromethyl)pyridin-3-yl)methyl]-1,2- cyclopenta[d]imidazol-4-yl]-1,2-oxazole-5-carboxamide oxazole-5-carboxylic acid 145 3-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 3-[(6-{6,6-Difluoro-3- (difluoromethyl)pyridin-3-yl)methyl]-N-[(4R)-1-methyl- azabicyclo[3.1.0]hexan-3-yl}-2- 1H,4H,5H,6H-cyclopenta-[d]imidazol-4-yl]-1,2-oxazole-5- (difluoromethyl)pyridin-3-yl)methyl]-1,2- carboxamide oxazole-5-carboxylic acid 146 3-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-ethylpyridin-3- 3-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- ethylpyridin-3-yl)methyl]-1,2-oxazole-5- cyclopenta[d]imidazol-4-yl]-1,2-oxazole-5-carboxamide carboxylic acid 147 3-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 3-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]- (hydroxymethyl)pyridin-3-yl)methyl]-N-[(4R)-1-methyl- hexan-3-yl}-2-(hydroxymethyl)pyridin-3- 1H,4H,5H,6H-cyclopenta-[d]imidazol-4-yl]-1,2-oxazole-5- yl)methyl]-1,2-oxazole-5-carboxylic acid carboxamide 148 3-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-chloropyridin-3- 3-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- chloropyridin-3-yl)methyl]-1,2-oxazole- cyclopenta[d]imidazol-4-yl]-1,2-oxazole-5-carboxamide 5-carboxylic acid 149 3-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(hydroxymethyl)- 3-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- (hydroxymethyl)pyridin-3-yl)methyl]-1,2- cyclopenta[d]imidazol-4-yl]-1,2-oxazole-5-carboxamide oxazole-5-carboxylic acid 150 3-[(2-Chloro-6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3- 3-[(2-Chloro-6-{6,6-difluoro-3- yl}pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- cyclopenta-[d]imidazol-4-yl]-1,2-oxazole-5-carboxamide yl)methyl]-1,2-oxazole-5-carboxylic acid 151 3-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}pyridin-3-yl)methyl]- 3-[(6-{3-Azabicyclo[3.1.0]hexan-3- N-[(4R)-1-methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4- yl}pyridin-3-yl)methyl]-1,2-oxazole-5- yl]-1,2-oxazole-5-carboxamide carboxylic acid 152 3-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 3-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]- methylpyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- hexan-3-yl}-2-methylpyridin-3- cyclopenta[d]imidazol-4-yl]-1,2-oxazole-5-carboxamide yl)methyl]-1,2-oxazole-5-carboxylic acid 153 3-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 3-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]- ethylpyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- hexan-3-yl}-2-ethylpyridin-3-yl)methyl]- cyclopenta[d]imidazol-4-yl]-1,2-oxazole-5-carboxamide 1,2-oxazole-5-carboxylic acid 154 2-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3- 2-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- methylpyridin-3-yl)methyl]-1,3-oxazole- cyclopenta[d]imidazol-4-yl]-1,3-oxazole-5-carboxamide 5-carboxylic acid 155 2-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 2-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]- methylpyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- hexan-3-yl}-2-methylpyridin-3- cyclopenta[d]imidazol-4-yl]-1,3-oxazole-5-carboxamide yl)methyl]-1,3-oxazole-5-carboxylic acid 156 3-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2-ethylpyridin-3- 3-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- ethylpyridin-3-yl)methyl]-1,2-oxazole-5- cyclopenta[d]imidazol-4-yl]-1,2-oxazole-5-carboxamidef carboxylic acid 157 3-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2-chloropyridin-3- 3-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- chloropyridin-3-yl)methyl]-1,2-oxazole- cyclopenta[d]imidazol-4-yl]-1,2-oxazole-5-carboxamide 5-carboxylic acid 158 2-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-ethylpyridin-3- 2-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- ethylpyridin-3-yl)methyl]-1,3-oxazole-5- cyclopenta[d]imidazol-4-yl]-1,3-oxazole-5-carboxamide carboxylic acid 159 5-[(2-Chloro-6-{6,6-difluoro-3-azabicyclo-[3.1.0]hexan-3- 5-[(2-Chloro-6-{6,6-difluoro-3- yl}pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- azabicyclo[3.1.0]hexan-3-yl}pyridin-3- cyclopenta[d]imidazol-4-yl]-1H-pyrrole-3-carboxamide yl)methyl]-1H-pyrrole-3-carboxylic acid 160 3-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 3-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]- ethylpyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- hexan-3-yl}-2-ethylpyridin-3-yl)methyl]- cyclopenta[d]imidazol-4-yl]-1-(propan-2-yl)-1H-pyrazole- 1-(propan-2-yl)-1H-pyrazole-5- 5-carboxamide carboxylic acid 161 3-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 3-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]- ethylpyridin-3-yl)methyl]-1-ethyl-N-[(4R)-1-methyl- hexan-3-yl}-2-ethylpyridin-3-yl)methyl]- 1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]-1H-pyrazole-5- 1-ethyl-1H-pyrazole-5-carboxylic acid carboxamide 162 3-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-ethylpyridin-3- 3-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- yl)methyl]-1-ethyl-N-[(4R)-1-methyl-1H,4H,5H,6H- ethylpyridin-3-yl)methyl]-1-ethyl-1H- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-5-carboxamide pyrazole-5-carboxylic acid 163 3-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 3-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]- methylpyridin-3-yl)methyl]-1-ethyl-N-[(4R)-1-methyl- hexan-3-yl}-2-methylpyridin-3- 1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]-1H-pyrazole-5- yl)methyl]-1-ethyl-1H-pyrazole-5- carboxamide carboxylic acid 164 3-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3- 3-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- yl)methyl]-1-ethyl-N-[(4R)-1-methyl-1H,4H,5H,6H- methylpyridin-3-yl)methyl]-1-ethyl-1H- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-5-carboxamide pyrazole-5-carboxylic acid 165 3-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-ethylpyridin-3- 3-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- ethylpyridin-3-yl)methyl]-1H-pyrazole-5- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-5-carboxamide carboxylic acid 166 3-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-ethylpyridin-3- 3-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- yl)methyl]-1-methyl-N-[(4R)-1-methyl-1H,4H,5H,6H- ethylpyridin-3-yl)methyl]-1-methyl-1H- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-5-carboxamide pyrazole-5-carboxylic acid 167 3-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 3-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]- methylpyridin-3-yl)methyl]-1-methyl-N-[(4R)-1-methyl- hexan-3-yl}-2-methylpyridin-3-yl)- 1H,4H,5H,6H-cyclopenta-[d]imidazol-4-yl]-1H-pyrazole-5- methyl]-1-methyl-1H-pyrazole-5- carboxamide carboxylic acid 168 3-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3- 3-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- yl)methyl]-1-methyl-N-[(4R)-1-methyl-1H,4H,5H,6H- methylpyridin-3-yl)methyl]-1-methyl-1H- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-5-carboxamide pyrazole-5-carboxylic acid 169 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2-methylpyridin-3- 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- methylpyridin-3-yl)methyl]-1H-pyrazole- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide 4-carboxylic acid 170 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2-methylpyridin-3- 1-[(6-{5-Azaspiro[2.3]hexan-5-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- methylpyridin-3-yl)methyl]-1H-imidazole- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide 4-carboxylic acid 171 1-[(2-{3-Azabicyclo[3.1.0]hexan-3-yl}-4-methylpyrimidin-5- 1-[(2-{3-Azabicyclo[3.1.0]hexan-3-yl}-4- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- methylpyrimidin-5-yl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide imidazole-4-carboxylic acid 172 1-[(2-{5-Azaspiro[2.3]hexan-5-yl}-4-methylpyrimidin-5- 1-[(2-{5-Azaspiro[2.3]hexan-5-yl}-4- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- methylpyrimidin-5-yl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide imidazole-4-carboxylic acid 173 7-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl]-2-methylpyridin-3- 7-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclopenta[d]- methylpyridin-3-yl)methyl]-7H-pyrrolo- imidazol-4-yl]-7H-pyrrolo[2,3-d]pyrimidine-5-carboxamide [2,3-d]pyrimidine-5-carboxylic acid 174 7-[(2-{3-Azabicyclo[3.1.0]hexan-3-yl}-4-methylpyrimidin-5- 7-[(2-{3-Azabicyclo[3.1.0]hexan-3-yl}-4- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclopenta[d]- methylpyrimidin-5-yl)methyl]-7H-pyrrolo- imidazol-4-yl]-7H-pyrrolo[2,3-d]pyrimidine-5-carboxamide [2,3-d]pyrimidine-5-carboxylic acid 175 7-[(2-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-4- 7-[(2-{6,6-Difluoro-3-azabicyclo[3.1.0]- methylpyrimidin-5-yl)methyl]-N-[(4R)-1-methyl- hexan-3-yl}-4-methylpyrimidin-5-yl)- 1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]-7H-pyrrolo[2,3- methyl]-7H-pyrrolo[2,3-d]pyrimidine-5- d]pyrimidine-5-carboxamide carboxylic acid 176 2-Chloro-1-[(6-{6,6-difluoro-3-azabicyclo-[3.1.0]hexan-3- 2-Chloro-1-[(6-{6,6-difluoro-3-aza- yl}pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- bicyclo[3.1.0]hexan-3-yl}pyridin-3-yl)- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide methyl]-1H-imidazole-4-carboxylic acid 177 1-[(2-{3-Azabicyclo[3.1.0]hexan-3-yl}-4-methylpyrimidin- 1-[(2-{3-Azabicyclo[3.1.0]hexan-3-yl}-4- 5-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- methylpyrimidin-5-yl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide pyrazole-4-carboxylic acid 178 1-[(2-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-4- 1-[(2-{6,6-Difluoro-3-azabicyclo[3.1.0]- methylpyrimidin-5-yl)methyl]-N-[(4R)-1-methyl- hexan-3-yl}-4-methylpyrimidin-5-yl)- 1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]-1H-1,2,3- methyl]-1H-1,2,3-triazole-4-carboxylic triazole-4-carboxamide acid 179 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]- (ethoxymethyl)pyridin-3-yl)methyl]-N-[(4R)-1-methyl- hexan-3-yl}-2-(ethoxymethyl)pyridin-3- 1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4- yl)methyl]-1H-pyrazole-4-carboxylic carboxamide acid 180 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(methoxymethyl)- 1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- (methoxymethyl)pyridin-3-yl)methyl]- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide 1H-imidazole-4-carboxylic acid 181 5-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3- 5-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- methylpyridin-3-yl)methyl]furan-2- cyclopenta[d]imidazol-4-yl]furan-2-carboxamide carboxylic 182 5-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3- 5-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- methylpyridin-3-yl)methyl]furan-3- cyclopenta[d]imidazol-4-yl]furan-3-carboxamide carboxylic acid 183 5-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-ethylpyridin-3- 5-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- ethylpyridin-3-yl)methyl]thiophene-2- cyclopenta[d]imidazol-4-yl]thiophene-2-carboxamide carboxylic acid 184 5-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 5-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]- ethylpyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- hexan-3-yl}-2-ethylpyridin-3-yl)- cyclopenta[d]imidazol-4-yl]thiophene-2-carboxamide methyl]thiophene-2-carboxylic acid 185 5-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 5-[(6-{6,6-Difluoro-3-azabicyclo- (hydroxymethyl)pyridin-3-yl)methyl]-N-[(4R)-1-methyl- [3.1.0]hexan-3-yl}-2-(hydroxymethyl)- 1H,4H,5H,6H-cyclopenta-[d]imidazol-4-yl]thiophene-2- pyridin-3-yl)-methyl]thiophene-2- carboxamide carboxylic acid 186 5-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 5-[(6-{6,6-Difluoro-3-azabicyclo- ethylpyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- [3.1.0]hexan-3-yl}-2-ethylpyridin-3- cyclopenta[d]imidazol-4-yl]furan-2-carboxamide yl)methyl]furan-2-carboxylic acid 187 5-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 5-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]- methylpyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- hexan-3-yl}-2-methylpyridin-3-yl)- cyclopenta[d]imidazol-4-yl]thiophene-2-carboxamide methyl]thiophene-2-carboxylic acid 188 2-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 2-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]- methylpyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- hexan-3-yl}-2-methylpyridin-3-yl)- cyclopenta[d]imidazol-4-yl]-1,3-thiazole-5-carboxamide methyl]-1,3-thiazole-5-carboxylic acid 189 5-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 5-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]- methylpyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- hexan-3-yl}-2-methylpyridin-3-yl)- cyclopenta[d]imidazol-4-yl]furan-2-carboxamide methyl]furan-2-carboxylic acid 190 1-[(4-{3-Azabicyclo[3.1.0]hexan-3-yl}-3- 1-[(4-{3-Azabicyclo[3.1.0]hexan-3-yl}-3- cyanophenyl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- cyanophenyl)methyl]-1H-pyrazole-4- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide carboxylic acid 191 1-[(4-{3-Azabicyclo[3.1.0]hexan-3-yl}-3,5- 1-[(4-{3-Azabicyclo[3.1.0]hexan-3-yl}- difluorophenyl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- 3,5-difluorophenyl)methyl]-1H-pyrazole- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide 4-carboxylic acid 192 2-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3,5- 2-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}- difluorophenyl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- 3,5-difluorophenyl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide imidazole-4-carboxylic acid 193 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3-cyano-2- 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3- methylphenyl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- cyano-2-methylphenyl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide pyrazole-4-carboxylic acid 194 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3-cyano-2- 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3- methylphenyl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- cyano-2-methylphenyl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide imidazole-4-carboxylic acid 195 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3-cyano-5- 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3- fluorophenyl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- cyano-5-fluorophenyl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide pyrazole-4-carboxylic acid 196 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3- 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3- cyanophenyl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- cyanophenyl)methyl]-1H-imidazole-4- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide carboxylic acid 197 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3,5- 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}- dicyanophenyl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- 3,5-dicyanophenyl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide pyrazole-4-carboxylic acid 198 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3-cyano-2- 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3- fluorophenyl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- cyano-2-fluorophenyl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide pyrazole-4-carboxylic acid 199 1-[(4-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 1-[(4-{6,6-difluoro-3-azabicyclo[3.1.0]- methylphenyl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- hexan-3-yl}-2-methylphenyl)methyl]- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide 1H-pyrazole-4-carboxylic acid 200 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-5-cyano-2- 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-5- methylphenyl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- cyano-2-methylphenyl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide pyrazole-4-carboxylic acid 201 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3-cyano-2- 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-3- fluorophenyl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- cyano-2-fluorophenyl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide imidazole-4-carboxylic acid 202 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-2-chloro-3- 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-2- cyanophenyl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- chloro-3-cyanophenyl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide pyrazole-4-carboxylic acid 203 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-2-chloro-3- 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-2- cyanophenyl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- chloro-3-cyanophenyl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide imidazole-4-carboxylic acid 204 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-2- 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-2- bromophenyl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- bromophenyl)methyl]-1H-imidazole-4- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide carboxylic acid 205 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-2- 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-2- methylphenyl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- methylphenyl)methyl]-1H-imidazole-4- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide carboxylic acid 206 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-2- 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-2- cyanophenyl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- cyanophenyl)methyl]-1H-imidazole-4- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide carboxylic acid 207 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-2-(hydroxymethyl)- 1-[(4-{3-azabicyclo[3.1.0]hexan-3-yl}-2- phenyl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- (hydroxymethyl)phenyl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide imidazole-4-carboxylic acid 208 1-[(2-cyano-4-{6,6-difluoro-3-azabicyclo-[3.1.0]hexan-3- 1-[(2-cyano-4-{6,6-difluoro-3-aza- yl}phenyl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- bicyclo[3.1.0]hexan-3-yl}phenyl)methyl]- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide 1H-pyrazole-4-carboxylic acid 209 1-[(2-bromo-4-{6,6-difluoro-3-azabicyclo-[3.1.0]hexan-3- 1-[(2-bromo-4-{6,6-difluoro-3-aza- yl}phenyl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- bicyclo[3.1.0]hexan-3-yl}phenyl)methyl]- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide 1H-pyrazole-4-carboxylic acid 210 1-[(2-cyano-4-{6,6-difluoro-3-azabicyclo-[3.1.0]hexan-3- 1-[(2-cyano-4-{6,6-difluoro-3-aza- yl}phenyl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- bicyclo[3.1.0]hexan-3-yl}phenyl)methyl]- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide 1H-imidazole-4-carboxylic acid 211 1-[(2-chloro-4-{6,6-difluoro-3-azabicyclo-[3.1.0]hexan-3- 1-[(2-chloro-4-{6,6-difluoro-3-aza- yl}phenyl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- bicyclo[3.1.0]hexan-3-yl}phenyl)methyl]- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide 1H-imidazole-4-carboxylic acid 212 1-[(4-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 1-[(4-{6,6-difluoro-3-azabicyclo[3.1.0]- fluorophenyl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- hexan-3-yl}-2-fluorophenyl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide imidazole-4-carboxylic acid 213 1-[(4-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2- 1-[(4-{6,6-difluoro-3-azabicyclo[3.1.0]- fluorophenyl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- hexan-3-yl}-2-fluorophenyl)methyl]-1H- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide pyrazole-4-carboxylic acid

Example 214

1-[(2-{3-Azabicyclo[3.1.0]hexan-3-yl}-4-(hydroxymethyl)pyrimidin-5-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide

[0830] ##STR00874##

[0831] To a mixture of 1-[(2-{3-azabicyclo[3.1.0]hexan-3-yl}-4-formylpyrimidin-5-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]-1H-imidazole-4-carboxamide (24 mg) in THF (2 mL) is added NaBH.sub.4. The mixture is stirred for 12 h at rt and then treated with aqueous HCl (1 M, 500 μL). After stirring for 10 minutes aqueous NaOH (1 M, 500 μL) is added. The mixture is diluted with MeOH and purified by HPLC on reversed phase (ACN, water) to give the title compound. LC (Method 1): t.sub.R=0.78 min; Mass spectrum (ESP): m/z=435 [M+H].sup.+.

Example 215

1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-(1-hydroxyethyl)pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]-1H-1,2,3-triazole-4-carboxamide

[0832] ##STR00875##

[0833] CH.sub.3MgBr (3 M in THF, 92 μL) is added dropwise under argon atmosphere to an ice-cooled mixture of 1-[(6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-formylpyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]-1H-1,2,3-triazole-4-carboxamide (13 mg) in THF (5 mL). The mixture is stirred for 12 h while warming to rt. Then the mixture is partitioned between saturated aqueous NH.sub.4Cl and EtOAc. The aqueous phase is extracted with EtOAc. The combined organic phases are dried (Na.sub.2SO.sub.4), concentrated in vacuo and the residue is purified by HPLC on reversed phase (ACN, water) to give the title compound.

[0834] LC (Method 1): t.sub.R=0.88 min; Mass spectrum (ESI.sup.+): m/z=485 [M+H].sup.+.

Example 216

1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3-yl)methyl]-3-(2-hydroxyethyl)-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide

[0835] ##STR00876##

[0836] LiAlH.sub.4 (1 M in THF, 200 μL) is added dropwise under argon atmosphere to a −78° C. cold mixture of methyl 2-{1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3-yl)methyl]-4-{[(4R)-1-methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]carbamoyl}-1H-pyrazol-3-yl}acetate (90 mg) in THF (1 mL). The mixture is stirred for 12 h while warming to rt. To the mixture are successively added water (14 μL), aqueous NaOH (4 M, 14 μL) and again water (14 μL). After vigorous stirring for 15 minutes the mixture is filtered over celite and the filter cake is washed with THF. The combined filtrates are concentrated in vacuo and the residue is purified by HPLC on reversed phase (ACN, water) to give the title compound. LC (Method 2): t.sub.R=0.66 min; Mass spectrum (ESP): m/z=462 [M+H].sup.+.

Example 217

1-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3-yl)methyl]-3-yl)hydroxymethyl)-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide

[0837] ##STR00877##

[0838] LiAlH.sub.4 (1 M in THF, 200 μL) is added dropwise under argon atmosphere to an ice-cooled mixture of methyl 1-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-methylpyridin-3-yl)methyl]-4-{[(4R)-1-methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]carbamoyl}-1H-pyrazole-3-carboxylate (25 mg) in THF (2 mL). The mixture is stirred for 30 minutes. To the mixture are successively added water (50 μL) and aqueous NaOH (4 M, 25 μL). After vigorous stirring for 15 minutes the mixture is filtered over celite and the filter cake is washed with THF. The combined filtrates are concentrated in vacuo and the residue is purified by HPLC on reversed phase (ACN, water) to give the title compound. LC (Method 1): t.sub.R=0.89 min; Mass spectrum (ESP): m/z=448 [M+H].sup.+.

Example 218

1-[(2-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-4-methylpyrimidin-5-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide

[0839] ##STR00878##

[0840] A mixture of 1-[(2-chloro-4-methylpyrimidin-5-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide (33 mg), 6,6-difluoro-3-azabicyclo[3.1.0]hexane hydrochloride (27 mg) and DIPEA (75 μL) in DMSO (1 mL) is stirred for 8 h at 60° C. After cooling to rt the mixture is diluted with DMSO and purified by HPLC on reversed phase (ACN, water) to give the title compound.

[0841] LC (Method 1): t.sub.R=0.86 min; Mass spectrum (ESI+): m/z=454 [M+H].sup.+.

Example 219

3-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(methoxymethyl)pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]-1H-pyrazole-5-carboxamide

[0842] ##STR00879##

[0843] 3-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2-(methoxymethyl)pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]-1-{[2-(trimethylsilyl)ethoxy]methyl}-1H-pyrazole-5-carboxamide and 5-[(6-{3-azabicyclo[3.1.0]hexan-3-yl}-2-(methoxymethyl)pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]-1-{[2-(trimethylsilyl)ethoxy]methyl}-1H-pyrazole-3-carboxamide (mixture of isomers) (20 mg) are dissolved in DCM (3 mL). Trifluoroacetic acid (1 mL) is added and the mixture is stirred for 12 h at rt. The mixture is then concentrated in vacuo, dissolved in MeOH (1 mL) and treated with NH.sub.3 (7 M in MeOH, 3 mL). The mixture is heated for 12 h to 80° C. in a sealed microwave vial. After cooling to rt the mixture is concentrated in vacuo and the residue is purified by HPLC on reversed phase (ACN, water) to give the title compound.

[0844] LC (Method 1): t.sub.R=0.91 min; Mass spectrum (ESI+): m/z=448 [M+H].sup.+.

[0845] Example 220 is prepared in analogy to Intermediate 219:

TABLE-US-00182 Mass spectrum (ESI+): m/z LC Example Structure t.sub.R [M + H].sup.+ Method 220 [00880] embedded image 0.87 434 Method 1

TABLE-US-00183 Example Name Name of Starting Material 220 3-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- 3-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- (hydroxymethyl)pyridin-3-yl)methyl]-N-[(4R)-1- (hydroxymethyl)pyridin-3-yl)methyl]-N-[(4R)-1- methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4- methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]- yl]-1H-pyrazole-5-carboxamide 1-{[2-(trimethylsilyl)ethoxy]methyl}-1H-pyrazole- 5-carboxamide and 5-[(6-{3-Azabicyclo[3.1.0]hexan-3-yl}-2- (hydroxymethyl)pyridin-3-yl)methyl]-N-[(4R)-1- methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]- 1-{[2-(trimethylsilyl)ethoxy]methyl}-1H-pyrazole- 3-carboxamide (mixture of isomers)

Example 221

1-[(6-{6,6-Difluoro-3-azabicyclo[3.1.0]hexan-3-yl}-2-methoxypyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide

[0846] ##STR00881##

[0847] A mixture of 1-[(2-chloro-6-{6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl}pyridin-3-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide (50 mg) and NaOCH.sub.3 (1 M in MeOH, 2 mL) is heated in a sealed microwave vial to 165° C. for 6 h. After cooling to rt the mixture is purified by HPLC on reversed phase (ACN, water) to give the title compound. LC (Method 2): t.sub.R=0.86 min; Mass spectrum (ESI+): m/z=470 [M+H].sup.+.

Example 222

1-[(2-{5-Azaspiro[2.3]hexan-5-yl}-4-methylpyrimidin-5-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide

[0848] ##STR00882##

[0849] A mixture of 1-[(2-chloro-4-methylpyrimidin-5-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide (40 mg), DIPEA (100 μL) and 5-azaspiro[2.3]hexane; trifluoroacetate (32 mg) in DMSO (2 mL) is stirred at 60° C. for 16 h. The mixture is purified by HPLC on reversed phase (ACN, water) to give the title compound. LC (Method 5): t.sub.R=0.56 min; Mass spectrum (ESP): m/z=419 [M+H].sup.+.

[0850] Examples 223 to 240 are prepared in analogy to example 222:

TABLE-US-00184 Mass spectrum (ESI+): m/z LC Example Structure t.sub.R [M + H].sup.+ Method 223 [00883] embedded image 0.58 491 Method 5 224 [00884] 0.51 444 Method 5 225 [00885] 0.73 447 Method 5 226 [00886] 0.62 433 Method 5 227 [00887] 0.69 433 Method 5 228 [00888] 0.72 477 Method 5 229 [00889] 0.67 433 Method 5 230 [00890] 0.65 433 Method 5 231 [00891] 0.46 435 Method 5 232 [00892] 0.75 447 Method 5 233 [00893] embedded image 0.61 463 Method 5 234 [00894] 0.67 477 Method 5 235 [00895] 0.44 449 Method 5 236 [00896] 0.42 435 Method 5 237 [00897] 0.76 447 Method 5 238 [00898] 0.67 433 Method 5 239 [00899] 0.71 447 Method 5 240 [00900] 0.71 447 Method 5

TABLE-US-00185 Example Name Name of Starting Material 223 1-[(2-{2-Hydroxy-2-methyl-7-azaspiro[3.5]nonan-7-yl}-4- 2-Methyl-7-azaspiro[3.5]nonan-2-ol methylpyrimidin-5-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- hydrochloride cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide 224 1-({2-[(1R,5S,6S)-6-Cyano-3-azabicyclo[3.1.0] hexan-3-yl]-4- (1R,5S,6S)-3-Azabicyclo[3.1.0] methylpyrimidin-5-yl}methyl)-N-[(4R)-1-methyl-1H,4H,5H,6H- hexane-6-carbonitrile hydrochloride cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide 225 1-({2-[(1R,5S)-1,5-Dimethyl-3-azabicyclo[3.1.0]hexan-3-yl]-4- (1R,5S)-1,5-Dimethyl-3- methylpyrimidin-5-yl}methyl)-N-[(4R)-1-methyl-1H,4H,5H,6H- azabicyclo[3.1.0]hexane cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide hydrochloride 226 1-[(2-{2-Azaspiro[3.3]heptan-2-yl}-4-methylpyrimidin-5- Bis(2-azaspiro[3.3]heptane) oxalic yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- acid cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide 227 1-[(2-{3-Azabicyclo[4.1.0]heptan-3-yl]-4-methylpyrimidin-5- 3-Azabicyclo[4.1.0]heptane yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- hydrochloride cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide 228 1-[(2-{6,6-Dimethyl-3-azabicyclo[3.1.0]hexan-3-yl}-4- 6,6-Dimethyl-3- methylpyrimidin-5-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- azabicyclo[3.1.0]hexane cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide 229 N-[(4R)-1-Methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]-1- (1R,5S,6R)-6-Methyl-3-azabicyclo- ({4-methyl-2-[(1R,5S,6R)-6-methyl-3-azabicyclo[3.1.0]hexan- [3.1.0]hexanehydrochloride 3-yl]pyrimidin-5-yl}methyl)-1H-pyrazole-4-carboxamide (Obtained by separation of the diastereomers of tert-butyl 6-methyl- 3-azabicyclo[3.1.0]hexane-3- carboxylate by standard RP chromatography and cleavage of the protecting group with HCl in EtOAc) 230 1-[(2-{5-Azaspiro[2.4]heptan-5-yl}-4-methylpyrimidin-5- 5-Azaspiro[2.4]heptane hydrochloride yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide 231 N-[(4R)-1-Methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]-1- 6-Oxa-3-azabicyclo[3.1.1]heptane; 4- [(4-methyl-2-{6-oxa-3-azabicyclo[3.1.1]heptan-3-yl}pyrimidin- methylbenzene-1-sulfonic acid 5-yl)methyl]-1H-pyrazole-4-carboxamide 232 1-[(2-{5-Azaspiro[2.5]octan-5-yl}-4-methylpyrimidin-5- 5-Azaspiro[2.5]octane yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide 233 1-[(2-{6-Methoxy-3-azabicyclo[3.1.1]heptan-3-yl}-4- 6-Methoxy-3-azabicyclo methylpyrimidin-5-yl)methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- [3.1.1]heptane hydrochloride cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide 234 1-({2-[(1R,5S,8R)-8-Methoxy-3-azabicyclo[3.2.1]octan-3-yl]-4- (1R,5S,8S)-8-Methoxy-3- methylpyrimidin-5-yl}methyl)-N-[(4R)-1-methyl-1H,4H,5H,6H- azabicyclo[3.2.1]octane cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide hydrochloride 235 1-({2-[(1R,5S,6S)-6-(Hydroxymethyl)-3-azabicyclo[3.1.0]- [(1R,5S,6S)-3- hexan-3-yl]-4-methylpyrimidin-5-yl}methyl)-N-[(4R)-1-methyl- Azabicyclo[3.1.0]hexan-6-yl]methanol 1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4- carboxamide 236 1-({2-[(1R,5S,6S)-6-Hydroxy-3-azabicyclo[3.1.0]hexan-3-yl]-4- (1R,5S,6S)-3- methylpyrimidin-5-yl}methyl)-N-[(4R)-1-methyl-1H,4H,5H,6H- Azabicyclo[3.1.0]hexan-6-ol cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide hydrochloride 237 1-[(2-{6-Azaspiro[2.5]octan-6-yl}-4-methylpyrimidin-5-yl)- 6-Azaspiro[2.5]octane hydrochloride methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide 238 1-[(2-{3-Azabicyclo[3.2.0]heptan-3-yl}-4-methylpyrimidin-5-yl)- 3-Azabicyclo[3.2.0]heptane methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- hydrochloride cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide 239 N-[(4R)-1-Methyl-1H,4H,5H,6H-cyclopenta[d]imidazol-4-yl]-1- Octahydrocyclopenta[c]pyrrole [(4-methyl-2-{octahydrocyclopenta[c]pyrrol-2-yl}pyrimidin-5- yl)methyl]-1H-pyrazole-4-carboxamide 240 1-[(2-{6-Azaspiro[3.4]octan-6-yl}-4-methylpyrimidin-5-yl)- 6-Azaspiro[3.4]octane methyl]-N-[(4R)-1-methyl-1H,4H,5H,6H- cyclopenta[d]imidazol-4-yl]-1H-pyrazole-4-carboxamide

HETEROAROMATIC CARBOXAMIDE DERIVATIVES AS PLASMA KALLIKREIN INHIBITORS

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C07D401/14

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A61K31/519

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C07D409/14

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A61K45/06

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C07D405/14

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A61K31/506

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A61K31/4439

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A01N43/00

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A61K31/519

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A61K45/06

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C07D401/14

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C07D403/14

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C07D413/14

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Abstract

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