Strain of the entomopathogenic fungus beauveria bassiana for protecting agricultural crops against crop-destroying insects and ticks

Abstract

The proposed solution relates to agricultural microbiology and biotechnology. A strain of the entomopathogenic fungus Beauveria bassiana 17HA has been deposited with the Russian National Collection of Industrial Microorganisms under number VKPM F-1526. The VKPM F-1526 strain of the entomopathogenic fungus Beauveria bassiana can be used to create a broad-spectrum microbiological insecticide suitable for treating different agricultural crops. The use of the strain makes it possible to increase crops yield.

Claims

1. A strain of the entomopathogenic fungus Beauveria bassiana deposited in the National Bioresource Center All-Russian Collection of Industrial Microorganisms NRC “Kurchatov Institute”—GosNIIGenetika under the registration number VKPM F-1526 for the protection of agricultural plants against insects and ticks—plant pests.

Description

EXAMPLE 1

[0030] A large-scale field experiment to identify the effectiveness of the claimed strain of Beauveria bassiana 17HA was carried out in 2017 in Uzbekistan in the Urtachirchik district of the Tashkent region on the “QURBONALI AGRO” farm on cotton crops in the fight against cotton bollworm, aphids and spider mites. For processing was used a deep culture of the fungus, obtained on Czapek-Dox medium with yeast extract, with a titer of 1×10.sup.8 blastospores/ml and a consumption rate of 3 l/ha. The introduction of the biological agent was carried out by spraying (the flow rate of the working fluid was 300 l/ha). The area of experimental plots was 1 hectare for each variant of the experiment in triplicate. Accounting for the number of pests was carried out before the treatment of plants (preliminary accounting), then on days 3, 7 and 14 after spraying. The results are presented in Table 1. The biological effectiveness of Beauveria bassiana 17HA in the fight against cotton bollworm, aphids and spider mites on cotton was 80.4%, 84.9% and 91.6%, respectively, which indicates a high degree of pathogenicity of the strain Beauveria bassiana 17HA in relation to various insects and mites—pests of plants and the absence of a pronounced specialization.

EXAMPLE 2

[0031] Field tests of the effectiveness of Beauveria bassiana 17HA were carried out in 2017 in the Rostov region on onions against tortricid onion moth larvae and on potatoes against potato moth caterpillars. For processing was used a deep culture of the fungus, obtained on Czapek-Dox medium with yeast extract, with a titer of 1×10.sup.8 blastospores/ml and a consumption rate of 3 l/ha. In both cases, 2 treatments were carried out during the growing season by spraying with a working fluid consumption rate of 300 l/ha with an interval of 10 days. Accounting for the number of pests was carried out before the treatment of plants (preliminary accounting), then on days 3, 7 and 10 after spraying. The results are presented in Tables 2 and 3. In both cases, high rates of effectiveness of the use of Beauveria bassiana 17HA in controlling the number of tortricid onion moth and potato moth larvae were achieved.

TABLE-US-00001 TABLE 1 Biological effectiveness of Beauveria bassiana 17HA against cotton bollworm, aphids and spider ticks on cotton. Cotton bollworm Average number of pests per plant, ind. Biological After processing, efficiency by Experi- Before by days days of ment pro- of accounting accounting, % Variant cessing 3 7 14 3 7 14 B. 9.7 4.6 2 1 47.8 72.4 80.4 bassiana 17HA Control 9.9 9 7.4 5.2 — — — (no pro- cessing) Aphids Average number of pests per plant, ind. Biological efficiency Before After processing, by by days pro- days of accounting of accounting, % cessing 3 7 14 3 7 14 19.8 10.4 4.1 1.9 40.6 73.4 84.9 19 16.8 14.8 12.1 — — — Spider tick Average number of pests per 1 leaf, ind. Biological efficiency Before After processing, by by days of pro- days of accounting accounting, % cessing 3 7 14 3 7 14 19.4 11.5 4.7 1.3 40.1 72.5 91.6 20 19.8 17.6 16 — — —

TABLE-US-00002 TABLE 2 Biological efficacy of Beauveria bassiana 17HA against onion moth on onions. First processing Average number of caterpillars per plant After Decrease in the number processing, relative to the original, by days of adjusted for control, by Experiment Before accounting days of registration, % Variant processing 3 7 10 3 7 10 B. bassiana 3.2 2.7 2.2 1.4 29.0 48.9 72.0 17HA Control 3.2 3.8 4.3 5.0 — — — (no pro- cessing) Second processing Average number of caterpillars per plant Decrease in the After processing, number relative to the Before by days of original, adjusted for control, second accounting by days of registration, % processing 3 7 10 3 7 10 1.4 1.1 1.0 0.9 79.3 83.4 85.8 5.0 5.3 6.0 6.3 — — —

TABLE-US-00003 TABLE 3 Biological efficacy of Beauveria bassiana 17HA against potato moth on potatoes. First processing Average number of caterpillars per plant Decrease in the After number relative processing, to the original, by days of adjusted for control, by Experiment Before accounting days of registration, % Variant processing 3 7 10 3 7 10 B. bassiana 3.4 2.7 2.2 1.4 32.5 51.1 73.1 17HA Control 3.4 4.0 4.5 5.2 — — — (no pro- cessing) Second processing Average number of caterpillars per plant Decrease in the number After processing, relative to the original, Before by days of adjusted for control, by second accounting days of registration, % processing 3 7 10 3 7 10 1.4 1.1 1.0 0.8 80.4 83.9 87.5 5.2 5.6 6.2 6.4 — — —