Abstract
A method of synthesizing core-shell nanohybrids is described herein. The method includes providing first nanoparticles and second nanoparticles in a liquid medium at a pH at which the first nanoparticles are neutral and the second nanoparticles are negatively charged, allowing the first nanoparticles to homoaggregate and form a core of at least one of the first nanoparticles, and allowing the second nanoparticles to heteroaggregate with the homoaggregated first nanoparticles to form a shell on the core so as to provide the core-shell nanohybrids. A nanohybrid is additionally described herein, which includes a core including at least one neutral nanoparticle within a shell containing charged nanoparticles, wherein the shell further includes nanogaps configured to allow access of substrates to the core.
Claims
1. A method of forming core-shell nanohybrids, said method comprising: providing first nanoparticles and second nanoparticles in a liquid medium at a pH at which the first nanoparticles are neutral and the second nanoparticles are negatively or positively charged; allowing the first nanoparticles to homoaggregate; forming a core of at least one of the first nanoparticles; and allowing the second nanoparticles to heteroaggregate with the homoaggregated first nanoparticles to form a shell on the core so as to provide the core-shell nanohybrids.
2. The method of claim 1, which is conducted at a temperature of 20-25° C.
3. The method of claim 1, which is conducted without organic reducing agents, organic solvents, organic ligands, or organic surfactants.
4. The method of claim 3, which is conducted without stirring.
5. The method of claim 1, wherein a base or an acid is added to the liquid medium to adjust the pH of the liquid medium to a pH point of zero charge of the first nanoparticles.
6. The method of claim 5, wherein the liquid medium is an aqueous solution.
7. The method of claim 1, wherein the first nanoparticles and the second nanoparticles are two different members selected from the group consisting of carbon nanotubes, cadmium telluride nanoparticles, graphene nanoparticles, magnetite nanoparticles, molybdenum disulfide nanoparticles, silver nanoparticles, palladium nanoparticles, gold nanoparticles, silicon nanoparticles, titanium oxide nanoparticles, and quantum dots.
8. The method of claim 1, wherein the first nanoparticles are hematite nanoparticles and the second nanoparticles are carboxylated polystyrene nanoparticles.
9. The method of claim 1, further comprising adjusting a concentration ratio of the second nanoparticles to the first nanoparticles to adjust a size of the core-shell nanohybrids.
10. The method of claim 1, wherein a suspension of the core-shell nanohybrids is free of unaggregated first nanoparticles and unaggregated second nanoparticles without conducting a purification step.
11. The method of claim 10, wherein a concentration ratio of the second nanoparticles to the first nanoparticles is minimized to form the suspension of the core-shell nanohybrids free of unaggregated first nanoparticles and unaggregated second nanoparticles.
12. The method of claim 1, wherein the core comprises only one of the first nanoparticles.
13. The method of claim 1, wherein the shell comprises nanogaps configured to allow access of substrates to the core.
14. A nanohybrid comprising a core comprising at least one neutral nanoparticle within a shell comprising charged nanoparticles, wherein the shell further comprises nanogaps configured to allow access of substrates to the core.
15. The nanohybrid of claim 14, wherein the shell comprises either positively charged nanoparticles or negatively charged nanoparticles.
16. The nanohybrid of claim 14, wherein the at least one neutral nanoparticle and the charged nanoparticles are members selected from the group consisting of carbon nanotubes, cadmium telluride nanoparticles, graphene nanoparticles, magnetite nanoparticles, molybdenum disulfide nanoparticles, silver nanoparticles, palladium nanoparticles, gold nanoparticles, silicon nanoparticles, titanium oxide nanoparticles, and quantum dots.
17. The nanohybrid of claim 14, wherein the at least one neutral nanoparticle comprises hematite nanoparticles and the charged nanoparticles comprise carboxylated polystyrene nanoparticles.
Description
BRIEF DESCRIPTION OF THE DRAWINGS
[0040] The invention will be described in conjunction with the following drawings, wherein:
[0041] FIG. 1A is a TEM image of primary HemNPs.
[0042] FIG. 1B is a graph of Zeta potential of 4.4 mg/L HemNPs at different pH.
[0043] FIG. 1C shows growth curves of hydrodynamic diameter of 8.8 mg/L HemNPs during homoaggregation at different pH over time. The inset of FIG. 1C is a TEM image of primary HemNPs.
[0044] FIG. 1D is a graph of the initial growth rate (nm/min) of hydrodynamic diameter of HemNPs at different pH, wherein error bars represent the standard deviations of at least duplicate samples. All experiments were performed at 1 mM NaCl.
[0045] FIG. 2A is a TEM image of primary 43 nm carboxylated PSNPs.
[0046] FIG. 2B is a graph of hydrodynamic diameter (D.sub.h) measurement of 43 nm and 107 nm carboxylated PSNPs at pH 6.3. All experiments were performed at 1 mM NaCl.
[0047] FIG. 3A is a graph showing growth and stabilization of hydrodynamic diameter during heteroaggregation of 8.8 mg/L HemNPs with varied concentrations of 43 nm carboxylated PSNPs. Schematics of HemNP-PSNP core-shell structures are also shown.
[0048] FIG. 3B is graph showing stabilized hydrodynamic diameter and zeta potential of core-shell nanohybrids as a function of the concentration of 43 nm carboxylated PSNPs. The error bars represent the standard deviations of duplicate experiments. All the experiments were conducted at 1 mM NaCl and pH 6.3.
[0049] FIG. 4 is a graph showing growth of hydrodynamic diameter during heteroaggregation of 8.8 mg/L HemNPs with 0.84 mg/L and 0.77 mg/L 43 nm carboxylated PSNPs at 1 mM NaCl and pH 6.3.
[0050] FIGS. 5A, 5B, 5C, and 5D are TEM images of core-shell nanohybrids collected in the stable stage of heteroaggregation of 8.8 mg/L HemNPs with (A, C) 4.2 mg/L 43 nm PSNPs and (B, D) 0.91 mg/L 43 nm PSNPs. The large and dark particles are HemNPs, and the small and light particles are PSNPs.
[0051] FIGS. 6A and 6B are bar graphs showing the distribution of longest diameter of the core-shell nanohybrids formed in the stable stage of the heteroaggregation of 8.8 mg/L HemNPs with (A) 4.2 mg/L 43 nm PSNPs and (B) 0.91 mg/L 43 nm PSNPs based on TEM images.
[0052] FIGS. 6C and 6D are bar graphs showing the distribution of the number of HemNPs in the cores of the core-shell nanohybrids formed in the stable stage of the heteroaggregation of 8.8 mg/L HemNPs with (C) 4.2 mg/L 43 nm PSNPs and (D) 0.91 mg/L 43 nm PSNPs based on TEM images.
[0053] FIG. 7A is a graph showing the growth of hydrodynamic diameters during the heteroaggregation of 8.8 mg/L HemNPs with varied concentrations of 107 nm carboxylated PSNPs.
[0054] FIG. 7B is a graph showing hydrodynamic diameter and zeta potential of stabilized nanohybrids formed at different concentrations of 107 nm PSNPs. The error bar represents the standard deviation of duplicate experiments. All the experiments were conducted at 1 mM NaCl and pH 6.3.
[0055] FIGS. 8A and 8B are TEM images of core-shell nanohybrids formed in the stable stage of the heteroaggregation between 8.8 mg/L HemNPs and 4.2 mg/L 43 nm carboxylated PSNPs at 1 mM NaCl and pH 6.3. The large and dark particles are HemNPs and the small and light particles are PSNPs.
[0056] FIGS. 9A and 9B are TEM images of core-shell nanohybrids formed in the stable stage of the heteroaggregation between 8.8 mg/L HemNPs and 0.91 mg/L 43 nm carboxylated PSNPs at 1 mM NaCl and pH 6.3. The large and dark particles are HemNPs and the small and light particles are PSNPs.
[0057] FIG. 10 is a TEM image depicting the sharing of PSNPs between the core-shell nanohybrids formed during the heteroaggregation between 8.8 mg/L HemNPs and 0.91 mg/L carboxylated PSNPs.
DETAILED DESCRIPTION OF PREFERRED EMBODIMENTS OF THE INVENTION
[0058] In this invention, a self-assembling method through heteroaggregation between neutral and charged nanoparticles is created for the formation of core-shell nanohybrids. The inventive method requires no organic solvent and extremely low energy input. In the method, NPs self-assemble into core-shell structures through Brownian motion. The size of core-shell nanohybrids can be controlled by changing the concentration ratio of neutral to charged NPs. Thus, a particularly preferred embodiment of the invention is an environmentally friendly, technically simple, and economical method for fabricating core-shell nanohybrids.
[0059] The inventive method does not require any organic chemicals such as reducing agents, ligands, or surfactants. In addition, the fabrication of core-shell nanohybrids using the inventive method is preferably done at room temperature (e.g., 20-25° C.) and requires minimum energy for mixing whereas the existing methods such as the hydrothermal method (38), the Stober method (39), the pyrolysis method (40, 41), and sol-gel method (42) require the temperature to increase to 400-600° C., 120° C., 265-280° C., and 70° C., respectively. Thus, the inventive method is much more energy efficient than existing methods.
[0060] In the inventive method, the core-shell nanohybrids are formed through spontaneous heteroaggregation between neutral nanoparticles and charged nanoparticles. When the two types of particles are mixed, homoaggregation of neutral nanoparticles and heteroaggregation between neutral nanoparticles and charged nanoparticles takes place. The heteroaggregation forms neutral NP-charged NP heteroaggregates. The size of heteroaggregates increased initially and became stable afterwards. The neutral NPs and charged NPs form core-shell structure in which neutral NPs are the cores and charged NPs are the shells.
[0061] The size of the core-shell nanohybrids can be controlled by varying the concentration ratio of charged NPs to neutral NPs. The core diameter of the core-shell nanohybrids can be controlled by this inventive method through changing the concentration ratio of the charged NPs to neutral NPs. The shell thickness can be controlled by selecting the size of charged NPs in the inventive method.
[0062] The neutral NPs and charged NPs can be made of any materials. The charged NPs in the heteroaggregation with neutral NPs to form core-shell nanohybrids can be either positively charged NPs or negatively charged NPs. The inventive method can also be utilized to synthesize core-shell nanohybrids having both positively and negatively charged NPs in the shell to serve multifunctional purposes.
[0063] A non-limiting exemplary list of materials for the neutral NPs and charged NPs includes: carbon nanotubes, cadmium telluride NPs, graphene NPs, magnetite NPs, molybdenum disulfide nanoparticles, silver NPs, palladium NPs, gold NPs, silicon NPs, titanium oxide NPs, quantum dots, etc. A preferred embodiment includes hematite NPs (HemNPs) as the neutral NPs and polystyrene NPs (PSNPs) as the charged NPs.
[0064] Any nanoparticles of interest can be made neutral by adjusting the pH of the solution to the pH point of zero charge (pH.sub.pzc) of that nanoparticle. For example, pH.sub.pzc of TiO.sub.2 NPs is ˜6.8 (43), pH.sub.pzc of SiO.sub.2 NPs is ˜2.1 (44), pH.sub.pzc of silver NPs is ˜7 (45), pH.sub.pzc of CuO NPs is ˜9 (46), pH.sub.pzc of graphene oxide NPs is ˜3 (46), pH.sub.pzc of carbon nanotubes is ˜7.10 (47), pH.sub.pzc of multiwalled carbon nanotubes is ˜5.43 (48), pH.sub.pzc of magnetite NPs is ˜7.90 (49), pH.sub.pzc of palladium NPs is ˜7.80 (50), and pH.sub.pzc of ZnO NPs is ˜9.30 (51). At pH values other than the pH.sub.pzc the nanoparticles of interest are either positively or negatively charged. Usually, NPs will be negatively charged at the pH higher than its pH.sub.pzc and positively charged at the pH lower than its pH.sub.pzc. If the pH is properly adjusted so that it is the pH.sub.pzc of a first certain material NPs but much different from the pH.sub.zpc of a second different material NPs, at this specific pH the first NPs are the neutral NPs and the second NPs are the charged NPs. In a preferred embodiment, the pH of a 1 mM NaCl dispersant solution was adjusted to 6.34 so that hematite nanoparticles (HemNPs) were neutral and underwent favorable homoaggregation whereas polystyrene nanoparticles (PSNPs) were negatively charged and underwent no homoaggregation.
[0065] In a preferred embodiment when HemNPs and PSNPs were mixed, homoaggregation of HemNPs and heteroaggregation between HemNPs and charged PSNPs took place simultaneously, forming neutral NP-charged NP heteroaggregates. As seen in FIG. 3A, the size of heteroaggregates increased initially and became stable afterwards, as shown by the hydrodynamic diameter measurement using dynamic light scattering (DLS). The transmission electron microscopy (TEM) images of heteroaggregates in FIGS. 5A and 5B at the stable stage show that neutral HemNPs and charged PSNPs formed core-shell structure in which neutral HemNPs are the cores and charged PSNPs are the shells. As seen in FIGS. 5B and 5D, at the lowest concentration ratio of charged nanoparticles to neutral nanoparticles, the colloidally stable core-shell nanohybrids could still form though heteroaggregation (e.g., at 0.91 mg/L PSNPs). The suspension after heteroaggregation stabilization only contained core-shell nanohybrids without impurities of individual charged or neutral particles. When the ratio of charged to neutral nanoparticles is controlled at a minimum level that is still high enough for self-stabilized heteroaggregation, preferred embodiments of the method of the invention will not require additional purification steps.
[0066] As seen in FIG. 3B, the elevated concentration of charged PSNPs reduced the size of the colloidally stable nanohybrids. An elevated concentration of charged PSNPs appears to allow the charged PSNPs to quickly surround neutral HemNPs before the neutral HemNPs had the chance to attach to other neutral HemNPs. Decreasing the concentration of the charged PSNPs resulted in a larger size of stable nanohybrids because neutral HemNPs could attach to each other through direct contact or bridging by an intermediate charged NP. The differences in the concentration of charged PSNPs and the size of the stable nanohybrids is seen in comparing the TEM images of FIGS. 5A and 5B, which show the HemNP-PSNP core-shell nanohybrids at 8.8 mg/L HemNPs and 4.2 mg/L PSNPs and 0.91 mg/L PSNPs, respectively.
[0067] An advantage of nanohybrids produced using the inventive method is that there are nanogaps in the shell in the core-shell nanohybrids. The nanogaps in the shell can allow the access of the substrates to the core, which is critical for the reaction of substrates with the core material to take place in some applications and for the synergistic effect of the core and shell materials. The core-shell nanohybrids with these nanogaps could be used in the field of catalytic oxidation and reduction, photocatalysis, environmental remediation, biosensors, bioimaging, drug delivery, etc.
[0068] The invention will be illustrated in more detail with reference to the following Examples, but it should be understood that the present invention is not deemed to be limited thereto.
EXAMPLES
[0069] Materials and Methods
[0070] Materials. HemNP stock suspension was synthesized through the forced hydrolysis of FeCl.sub.3 (34, 35). Carboxylated PSNPs of two different sizes (i.e., 43 nm and 107 nm) were purchased from Polysciences, Inc. The deionized (DI) water (Millipore, MA) used in this study had a resistivity of 18.2 MSΩ.Math.cm.
[0071] Estimation of NP Number Concentrations. The number concentration of NPs was estimated by dividing the mass concentration of NPs by the mass of an individual NP, which was calculated by multiplying the volume of NP by the density of material (i.e., 5300 kg/m.sup.3 for hematite and 1040 kg/m.sup.3 for polystyrene). The volume of NP was calculated assuming it is spherical, and its diameter is equal to the average hydrodynamic diameter of primary NPs measured by dynamic light scattering (DLS).
[0072] Zeta Potential Measurements. Zeta potential of NPs (i.e., HemNPs and PSNPs) and core-shell nanohybrids were measured using the dip cell (ZEN1002) in a Zetasizer Nano ZS90 (Malvern). The scattered light intensities of the highest concentrations of PSNPs, i.e., 4.2 mg/L 43 nm PSNPs and 14 mg/L 107 nm PSNPs were 1% and 36%, respectively, of that of 8.8 mg/L HemNPs. Thus, the zeta potential measurements in the stable stage of heteroaggregation between 8.8 mg/L HemNPs and various concentrations of PSNPs mainly represent the zeta potential of nanohybrids that contained HemNPs rather than individual PSNPs if there were any.
[0073] Aggregation Kinetics. Homoaggregation and heteroaggregation kinetics were studied by measuring the hydrodynamic diameter (D.sub.h) in real time using a Zetasizer Nano ZS90 (Malvern), with scattering angle selected as 90°; the measurement position fixed in the center of the cell; autocorrelation time reduced to 15 s; no delay between measurements; and the attenuation increased to the highest value (11).
[0074] The 1 ml 8.8 mg/L HemNPs suspension at 1 mM NaCl were prepared and sonicated (Branson M3800, output power 120 W, frequency 40 kHz) for five minutes to achieve an aggregate-free suspension where the HemNPs had the hydrodynamic diameter of 100 nm. Homoaggregation of HemNPs was initiated by adjusting the pH to the value of interest by introducing adequate amount of NaOH or HCl stock solution and homogenizing the suspension by brief hand swirling. Immediately after that, the suspension was transferred to the cuvette very quickly before continuous measurements of hydrodynamic diameter were started. For heteroaggregation experiments, all the steps were the same except for adding the predetermined volume of carboxylated PSNP stock suspension right after adjusting the pH. All aggregation experiments were at least duplicated.
[0075] Transmission Electron Microscopy. Structures of core-shell nanohybrids were studied using a transmission electron microscope (PHILIPS CM 200) in a bright field mode at 120 kV. Gold grids (ultrathin carbon film on lacey carbon support film, 300 mesh, TED PELLA, INC.) were firstly soaked in 0.1 g/L poly-L-lysine (PLL) for 30 minutes to create a positively charged PLL coating on the surface of the grid. Then, the grid was washed with DI water. Afterwards, the PLL-coated grid was soaked in the suspension of stable heteroaggregates of interest for about 1.5 hours. The suspensions of stable heteroaggregates were prepared by performing heteroaggregation experiments of interest for 120 min. Since the stable heteroaggregates were all negatively charged, they could readily attach to the positively charged PLL-coated grids during soaking. Superposition of different heteroaggregates on the grid was unlikely to happen due to the electrostatic repulsion between heteroaggregates in the stable stage.
[0076] Then, the grid was taken out of the heteroaggregate suspension and washed gently by dipping the grid in DI water several times to remove any particles that were loosely bound to the grid. After washing, the grid was air dried. As only the strongly attached heteroaggregates remained on the surface of the grids after washing, the DI water film on the grids was not likely to bring nanoparticles together and cause artifacts during the drying process. Thus, this method of collecting heteroaggregates on TEM grids should be able to retain the original heteroaggregate structure in the aqueous solution. Following the same procedure, the TEM grids for imaging primary HemNPs and PSNPs were also prepared.
Example 1—Hematite Nanoparticles (HemNPs) as Model Neutral NPs and Carboxylated Polystyrene Nanoparticles (PSNPs) as Model Charged NPs at pH 6.3
[0077] In this example, HemNPs were used as the model neutral NPs. As shown in FIG. 1A and the inset in FIG. 1C, HemNPs have spherical shape and the diameter of primary HemNPs is 75 nm on average as measured from those transmission electron microscopic (TEM) images. The point of zero charge (PZC) of HemNPs was determined by electrophoretic mobility measurement. FIG. 1B shows zeta potential of HemNPs at 1 mM NaCl as a function of pH, which progressively decreased with the increase of pH from 20 mV at pH 4.84 to −19 mV at the pH 7.2. The PZC of HemNPs is estimated to be pH 6.0 by interpolation. A decrease of pH from the PZC makes the zeta potential of HemNPs positive, and an increase of pH from the PZC makes their zeta potential negative. The standard deviation of zeta potential measurement is large when the pH is close to PZC due to the limitation of zeta potential analyzer when the electrophoretic mobility of nanoparticles is low.
[0078] To better determine the PZC of HemNPs from the perspective of colloidal stability, homoaggregation experiments of HemNPs were conducted at 1 mM NaCl and different pHs. The growth rate of HemNP homoaggregates is expected to be the highest at its PZC. The average hydrodynamic diameter (D.sub.h) of HemNPs before aggregation was 100 nm as determined by DLS. In this example, the size of NPs or nanohybrids refers to the average D.sub.h, unless otherwise stated. The homoaggregation experiments of HemNPs were performed from pH 5.8 to pH 7.2 in order to find the pH at which HemNPs have the most favorable homoaggregation. FIG. 1C shows the homoaggregation profile of 8.8 mg/L HemNPs in the presence of 1 mM NaCl at pH 5.8, 6.3, 6.7, and 7.2 for at least 45 minutes. At pH 5.8, D.sub.h shows no increase with time as the positively charged HemNPs were colloidally stable due to the electrostatic repulsion. At pH 6.3, 6.7, and 7.2, homoaggregation of HemNPs took place and resulted in the growth of D.sub.h with time. FIG. 1D shows the growth rate of HemNPs as a function of pH, calculated from the linear least-squares analysis of the growth of hydrodynamic diameters during the initial five minutes of homoaggregation. From pH 5.8, the initial growth rate of HemNPs increased with the increase of pH and reached maximum at pH 6.2±0.1 at which the surface charge of HemNPs should be very close to neutral, thus resulting in the fastest homoaggregation. A further increase of pH from this point resulted in decrease of growth rate due to that HemNPs became negatively charged as shown in FIG. 1B. Hence, in order to use HemNPs as model neutral NPs for the fabrication of core-shell nanohybrids, all heteroaggregation experiments were performed at pH 6.3±0.1.
[0079] Carboxylated PSNPs of two different sizes (i.e., 43 nm and 107 nm) were used as the model charged NPs. FIG. 2A shows the TEM image of 43 nm PSNPs which confirm the spherical shape of PSNPs. At pH 6.3, zeta potentials of 43 nm and 107 nm PSNPs were −39.6±3.9 mV (avg. ±SD.) and −43.5±0.4 mV (avg. ±SD.), respectively, in the presence of 1 mM NaCl. At the same solution chemistry, D.sub.h of both PSNPs maintained stable as shown in FIG. 2B due to electrostatic repulsion. The concentrations of 43 nm and 107 nm PSNPs used in this study were 0.91-4.2 mg/L and 5.6-14 mg/L, respectively, giving scattered light intensity of 4-16 kcps and 270-640 kcps, respectively, in DLS measurement, which are considerably lower than that of 8.8 mg/L HemNPs (1500 kcps). Thus, when PSNPs were mixed with 8.8 mg/L HemNPs at pH 6.3, the hydrodynamic diameter of mixed NPs measured by DLS mainly represents that of HemNPs and nanohybrids containing HemNPs without much interference by free PSNPs. Especially in the case of 43 nm PSNPs, free PSNPs can be considered “invisible” to the detector of DLS instrument. This method was also used in previous heteroaggregation studies (34, 36).
Example 2—Formation of Core-Shell Nanohybrids Through Self-Assembling of Neutral and Charged Nanoparticles in Heteroaggregation Process
[0080] Heteroaggregation experiments between model neutral NPs (e.g., HemNPs) and model charged NPs (e.g., carboxylated PSNPs) were conducted at 1 mM NaCl and pH 6.3. At this solution chemistry, HemNPs were neutral and could undergo not only favorable homoaggregation (FIG. 1C) but also heteroaggregation with PSNPs due to van der Waals forces whereas PSNPs were negatively charged and thus colloidally stable to homoaggregation due to electrostatic repulsion. The suspension containing the two types of NPs was homogenized by briefly swirling with hands and then left stationary to allow the heteroaggregation of NPs to take place through Brownian motions. FIG. 3A shows heteroaggregation profiles of 8.8 mg/L HemNPs with three different concentrations (i.e., 0.91, 1.4, and 4.2 mg/L) of 43 nm carboxylated PSNPs. At 4.2 mg/L PSNPs, the number ratio of HemNPs to PSNPs was estimated to be 1:30. D.sub.h increased initially (growth stage) due to the attachment of PSNPs to the surface of HemNPs. Then, it became stable at 133 nm on average (FIGS. 3A and 3B) after ˜80 min (stable stage) because sufficient amount of PSNPs had attached to each primary HemNP and formed core-shell structure with HemNPs as the cores and PSNPs as the shells as illustrated by the schematic in FIG. 3A. At a lower concentration of PSNPs, 1.4 mg/L, the number ratio of HemNPs to PSNPs was estimated to be 1:10. Since the number of PSNPs surrounding each HemNP has significantly dropped, there was a higher chance for a HemNP to attach to other HemNPs before the shell of PSNPs formed around the HemNP. Thus, homoaggregation of HemNPs and heteroaggregation between HemNPs and PSNPs may have taken place simultaneously, resulting in the initial increase in D.sub.h from 133 nm to 156 nm (FIG. 3A). D.sub.h stabilized after ˜100 min at 156 nm on average (FIGS. 3A and 3B) due to the formation of core-shell structure of HemNP-PSNP nanohybrids. The larger stabilized D.sub.h (FIG. 3B) is likely due to the formation of larger cores of HemNPs. A further decrease of the concentration of PSNPs to 0.91 mg/L (number ratio of HemNPs to PSNPs is 1:7) allowed more HemNPs to undergo homoaggregation before HemNP cores were shielded by PSNP shells, which resulted in further increase of the stabilized D.sub.h to 180 nm on average (FIGS. 3A and 3B). The zeta potential of stabilized nanohybrids became less negative from −40.8 to −33.8 mV as the concentration of the PSNPs decreased from 4.2 mg/L to 0.91 mg/L (FIG. 3B), probably due to that the shell of PSNPs became less compact when there were fewer PSNPs available in the suspension.
[0081] A further decrease of the concentration of PSNPs from 0.91 mg/L to 0.84 mg/L (HemNPs: PSNPs=1:6.1), and to 0.77 mg/L (HemNPs: PSNPs=1:5.6) in heteroaggregation resulted in a continuous increase of D.sub.h which never stabilized within the time frame of experiments (FIG. 4). As the concentration of PSNPs was too low, the number of PSNPs was not enough to form shells around and stabilize HemNPs. The continuous increase of D.sub.h of heteroaggregates was due to both homoaggregation of HemNPs and the heteroaggregation. The similar hypothesis on the formation of core-shell structure between neutral CeO.sub.2 NPs and negatively charged biochar NPs was proposed by Yi et al. (36) to explain the self-stabilization phenomena during their heteroaggregation.
[0082] In order to prove the hypothesis of formation of core-shell nanohybrids in the heteroaggregation process, the transmission electron microscopy (TEM) images of heteroaggregates (FIGS. 5A and 5B) collected in the stable stage were taken using the sample preparation method that can avoid the superposition of particles on the TEM grids during the drying process and thus retain the aggregate structure. As shown in FIG. 5A, the HemNP-PSNP core-shell structure indeed had formed in the stable stage of the heteroaggregation between 8.8 mg/L HemNPs and 4.2 mg/L 43 nm carboxylated PSNPs. FIGS. 5C, 8A and 8B provide broader views of particles existing in the suspension, in which a lot of free individual PSNPs can be seen in addition to core-shell nanohybrids. Particularly, FIGS. 8A and 8B show TEM images of core-shell nanohybrids formed in the stable stage of the heteroaggregation between 8.8 mg/L and 43 nm carboxylated PSNPs at 1 mM NaCl and pH 6.3. The large and dark particles visible in FIGS. 8A and 8B are HemNPs and the small and light particles are PSNPs. Due to the presence of abundant PSNPs, almost all the core-shell nanohybrids had only one HemNP as the core (FIG. 6C) which is surrounded by 3-7 PSNPs as the shell. The average longest diameter of nanohybrids is 110 nm based on the size distribution of all core-shell nanohybrids (FIG. 6A) found from eight TEM images.
[0083] FIGS. 5B, 5D, 9A, and 9B show the TEM images of heteroaggregate structure formed in the stable stage of heteroaggregation between 8.8 mg/L HemNPs and 0.91 mg/L 43 nm carboxylated PSNPs at 1 mM NaCl and pH 6.3. The large, dark particles visible in the TEM images are HemNPs and the smaller, lighter particles are PSNPs. The observation is also consistent with the hypothesis. Larger core-shell nanohybrids had formed with multiple (i.e., 1-8) HemNPs as the core (FIG. 6D) and PSNPs as the shell. In a few cases, the HemNPs in the core were connected through a bridging PSNP (FIG. 10) rather than direct contact. FIG. 10 shows a TEM image illustrating the sharing of PSNPs between the core-shell nanohybrids formed during the heteroaggregation between 8.8 mg/L HemNPs and 0.91 mg/L carboxylated PSNPs. In FIG. 10, the larger darker particles shown are HemNPs and the smaller lighter particles are PSNPs. There were almost no free individual PSNPs in the suspension (FIGS. 5B, 5D, 9A, and 9B). All PSNPs had bound to HemNPs, preventing HemNPs from growing through homoaggregation. The number ratio of HemNPs to PSNPs within the nanohybrids was 1:2-1:7, which is interestingly very similar to the ones formed at 4.2 mg/L PSNPs. The statistical analysis on the size of nanohybrids in seventeen TEM images revealed that the average longest diameter is 213 nm (FIG. 6B), which is much larger than the ones formed at 4.2 mg/L PSNPs, consistent with the findings in the measurement of their hydrodynamic diameters (FIG. 3).
Example 3—Effect of Size of Charged Nanoparticles on the Formation of Nanohybrids
[0084] The size of model charged nanoparticles (i.e., carboxylated PSNPs) was increased from 43 nm to 107 nm to study the influence of such size increase on the formation of nanohybrids with the same model neutral nanoparticles (i.e., 100 nm HemNPs) under the same solution chemistry (i.e., pH 6.3 and 1 mM NaCl). FIG. 7A shows the growth of hydrodynamic diameter during the heteroaggregation of 8.8 mg/L HemNPs with 107 nm carboxylated PSNPs at three different concentrations (i.e., 5.6, 7, 14 mg/L) where the number ratios of HemNPs to PSNPs are 1:2.6, 1:3.3, and 1:6.6, respectively. Similar to the heteroaggregation with 43 nm PSNPs, the heteroaggregation of HemNPs with 107 nm PSNPs sequentially had a growth stage and a stable stage. Thus, it is a reasonable speculation that HemNPs and 107 nm PSNPs had also formed core-shell nanohybrids with HemNPs as the core and PSNPs as the shell in the stable stage, as illustrated by the schematics in FIG. 7A. As the PSNP concentration decreased, the core of HemNPs had more opportunities to grow through homoaggregation before being surrounded and stabilized by PSNPs, thus resulting in the larger size of nanohybrids in the stable stage (FIG. 7B). The zeta potential of nanohybrids was less negative at 5.6 mg/L PSNPs compared to that at 7 or 14 mg/L (FIG. 7B), probably because there were fewer PSNPs attaching to each HemNP in the nanohybrids at 5.6 mg/L. It is also interesting to note that the number ratios of PSNPs to HemNPs required to form stabilized nanohybrids are much lower for 107 nm PSNPs (2.6-6.6) compared with that for 43 nm PSNPs (7-30), likely due to a larger PSNP shielding more surface area of the HemNP than a smaller PSNP. In addition, the size of the stabilized nanohybrids formed using 107 nm PSNPs is 220-440 nm which is larger than the nanohybrids (133-180 nm) formed using 43 nm PSNPs, probably ascribed to the thicker PSNP shells. The TEM images of nanohybrids of HemNPs and 107 nm PSNPs are not provided because the two NPs had similar darkness, shape, and size under the microscope, making it difficult to differentiate them.
CONCLUSION
[0085] Core-shell nanohybrids can be formed through spontaneous heteroaggregation between model neutral NPs (e.g., HemNPs) and model charged NPs (e.g., carboxylated PSNPs) under room temperature and stationary condition. It is expected that other types of neutral and charged nanospheres can also self-assemble into core-shell structures using the heteroaggregation method. In the heteroaggregation process, neutral particles formed the cores and charged particles formed the shells. The size of nanohybrids became larger when the number ratio of neutral to charged nanoparticles increased. When there were fewer charged particles around, neutral particles were more likely to grow to a larger core through homoaggregation before being surrounded and shielded by the charged particles. The number ratio of neutral to charged nanoparticles required for making stable nanohybrids, and the size of nanohybrids increased as the charged NPs became larger. The formation of core-shell nanohybrids through heteroaggregation requires no heating and almost no external mixing, which renders the process more energy efficient than existing methods of making core-shell nanohybrids.
[0086] While the invention has been described in detail and with reference to specific examples thereof, it will be apparent to one skilled in the art that various changes and modifications can be made therein without departing from the spirit and scope thereof.
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