METHOD OF PROTECTING HUMAN SKIN AGAINST DAMAGE UPON EXPOSURE WITH BLUE LIGHT

Abstract

The present invention relates to a method for the protection of human skin against visible light damages, namely blue light, said method comprising the step of applying a cosmetic composition containing an effective amount of vitamin B3 or a derivative thereof and an effective amount of vitamin B6 or derivative thereof to the skin.

Claims

1. A method of protecting human skin against damage upon exposure to blue light, said method comprising the step of applying a cosmetic composition containing an effective amount of Vitamin B.sub.3 or a derivative thereof and an effective amount of Vitamin B.sub.6 or a derivative thereof to the skin.

2. The method according to claim 1, wherein the Vitamin B.sub.3 or derivative thereof derivative is niacinamide and the Vitamin B.sub.6 or derivative thereof if pyridoxine hydrochloride.

3. The method according to claim 1, wherein the effective amount of the Vitamin B.sub.3 or derivative thereof is selected in the range of 0.5 to 10 wt. %, preferably in the range of 1 to 10 wt. %, most preferably in the range of 1 to 5 wt. %, based on the total weight of the composition.

4. The method according to claim 1, wherein the effective amount of the Vitamin B.sub.6 or derivative thereof is selected in the range of 0.02 to 6 wt. %, preferably in the range of 0.05 to 4 wt. %, most preferably in the range of 0.1 to 3 wt. %, based on the total weight of the composition.

5. A method according to claim 1, wherein the amount of niacinamide is higher than the amount of pyridoxine hydrochloride.

6. A method according to claim 1, wherein the damage is the result of the generation of reactive oxygen species, reactive nitrogen species and/or reactive carbonyl species.

7. The method according to claim 1, wherein the composition further comprises at least one UV-filter substance.

8. The method according to claim 7, wherein the at least one UV-filter substance is selected from the group consisting of polysilicone-15, phenyl benzimidazol sulfonic acid, octocrylene, ethylhexyl methoxycinnamate, ethylhexyl salicylate, homosalate, bis-ethylhexyloxyphenol methoxyphenyl triazine, methylene bis-benzotriazolyl tetramethylbutylphenol, titanium dioxide, butyl methoxydibenzoylmethane, diethylamino hydroxybenzoyl hexyl benzoate, disodium phenyl dibenzimidazole tetrasulfonate as well as mixtures thereof.

9. The method according to claim 1, wherein the composition is a skin care composition.

10. The method according to claim 1, wherein the composition is in the form of O/W emulsions comprising an oily phase dispersed in an aqueous phase in the presence of an O/W emulsifier.

11. The method according to claim 10, wherein the O/W emulsifier is potassium cetyl phosphate.

12. A method of reducing the generation of reactive oxygen species and/or the formation of carbonylated proteins in humans when exposed to blue light, said method comprising the step of applying a cosmetic composition containing an effective amount of niacinamide and pyridoxine hydrochloride to the skin.

13. A method according to claim 1, wherein the effective amount of the niacinamide in the cosmetic composition is selected in the range of 0.5 to 10 wt. %, and the amount of pyridoxine hydrochloride is selected in the range of 0.02 to 6 wt. %, based on the total weight of the cosmetic composition.

14. A combination of niacinamide and pyridoxine hydrochloride for the use in the protection of human skin against the adverse effects of electromagnetic radiation.

15. The use according to claim 14, wherein the adverse effects are the formation of reactive oxygen species and/or light induced oxidative stress.

Description

EXAMPLE

[0052] Following the paper of T. Rudolph et. al. (SOFW-Journal, 140, 3-2014), beta-carotene (DSM, β-Carotene Crystalline, Lot Nr. WC01503161) was dissolved in o-xylene to a concentration of 0.5% (w/w) and was homogenously applied over a PMMA plate (Schonberg, sandblasted, 2 μm roughness) with a syringe. After 10 min drying (RT, in the dark) the formulation as outlined in table 2 were applied (2 μLcm.sup.−2) and distributed with a finger. Per formulation 3 replicates were produced and irradiated. Parallel 3 replicates with placebo cream (no active) were prepared which were also irradiated. The irradiation took place in an Atlas SunTester XLS+ with a total irradiance of 500 Wm.sup.−2 with an irradiation dose of 3MED. On top of the PMMA plates a UV-cut-off filter has been placed. This UV-cut-off filter is a Schott GG400-3.

[0053] After irradiation, the respective PMMA plates were extracted with 50 mL isopropanol in an ultrasonic bath for 1 min and the residual amount of beta-carotene was photometrically quantified at 452 nm.

[0054] Then the protection (%) was determined using the following formula:

% Protection=([(Absorbance sample)*100]/[Absorbance placebo]−100)

[0055] (The absorbance at 452 nm after irradiation of the placebo (maximum degradation) was set to 0%).

TABLE-US-00001 TABLE 1 Results Sample (active) Protection* 1 (none, Placebo)  0% 2 (3% Niacinamide) +12% 3 (1% Pyridoxin HCl) +10% 4 (3% Niacinamid + 1% Pyridoxin HCl) +58% *Increase in % versus placebo (irradiated)

[0056] As can be retrieved from table 1, the addition of the combination of niacinamide and Pyridoxin hydrochloride to the placebo formulation led to a synergistic protection of the beta-carotene from blue light degradation.

TABLE-US-00002 TABLE 2 Sample INCI name 1 2 3 4 BIS-ETHYLHEXYLOXYPHENOL METHOXYPHENYL 1.5 1.5 1.5 1.5 TRIAZINE POLYSILICONE-15 1 1 1 1 BUTYL METHOXYDIBENZOYLMETHANE 2 2 2 2 ETHYLHEXYL SALICYLATE 5 5 5 5 OCTOCRYLENE 1.5 1.5 1.5 1.5 DIISOPROPYL SEBACATE 3 3 3 3 DIMETHICONE 2 2 2 2 DICAPRYLYL ETHER 2 2 2 2 DICAPRYLYL ETHER 2 2 2 2 TITANIUM DIOXIDE, SILICA, DIMETHICONE 3 3 3 3 CETYL PHOSPHATE 1.5 1.5 1.5 1.5 STEARYL ALCOHOL 3.15 3.15 3.15 3.15 HYDROXYETHYL ACRYLATE/SODIUM 0.5 0.5 0.5 0.5 ACRYLOYLDIMETHYL TAURATE COPOLYMER POLYACRYLATE CROSSPOLYMER-6 0.5 0.5 0.5 0.5 PHENOXYETHANOL, ETHYLHEXYLGLYCERIN 1 1 1 1 SILICA 3 3 3 3 AQUA Ad 100 PROPANEDIOL 5 5 5 5 TROMETHAMINE, AQUA 0.86 0.86 0.86 0.86 METHYLENE BIS-BENZOTRIAZOLYL 8 8 8 8 TETRAMETHYLBUTYLPHENOL, AQUA NIACINAMIDE 3 3 PYRIDOXIN HCl 1 1