Cell printing apparatus

Abstract

A cell printing apparatus according to the present disclosure comprises: a nozzle through which a liquid cell substance is discharged; a container in which the liquid cell substance discharged through the nozzle is laminated into a three-dimensional structure; and a supply unit for supplying the liquid heated to a predetermined temperature to the container.

Claims

1. A cell printing apparatus comprising: a nozzle configured to discharge a liquid cell substance; a container where the liquid cell substance discharged from the nozzle is laminated to create a 3D structure; and a supply unit for supplying a heated liquid to the container, wherein the heated liquid cures the liquid cell substance, wherein the supply unit is configured to regulate an amount of the heated liquid supplied to the container to cause a top portion of the 3D structure to be exposed and a remaining portion of the 3D structure to be submerged.

2. The cell printing apparatus according to claim 1, wherein the heated liquid is heated to a gelation temperature to gel the liquid cell substance discharged from the nozzle.

3. The cell printing apparatus according to claim 1 or 2, wherein the heated liquid is a cell culture medium.

4. The cell printing apparatus according to claim 1 or 2, wherein the supply unit starts to supply the heated liquid after a first layer of the liquid cell substance is laminated.

5. The cell printing apparatus according to claim 1 or 2, further comprising a heating unit for heating the container.

Description

BRIEF DESCRIPTION OF DRAWINGS

(1) FIG. 1 is a view showing a state in which a first layer is laminated by a cell printing apparatus according to an embodiment of the present disclosure.

(2) FIGS. 2 and 3 are views showing a state in which a heated liquid is supplied, in a state of being further laminated on the first layer in FIG. 1.

DETAILED DESCRIPTION

(3) Hereinafter, the present disclosure will be described in detail with reference to the accompanying drawings.

(4) FIG. 1 is a view schematically showing a state in which a first layer is laminated by a cell printing apparatus according to an embodiment of the present disclosure. As illustrated in FIG. 1, the cell printing apparatus of this embodiment comprises a nozzle unit (10) for discharging a liquid cell substance, a container (20) where a liquid cell substance discharged from the nozzle unit (10) is laminated to create a 3D structure (40), and a supply unit (30) for supplying a liquid (50) heated to a predetermined temperature into the container (20). The supply unit (30) has a controller (31), a liquid storage (34) and a supply pipe (35). In the supply unit (30) in FIG. 1 which is shown non-limitedly and exemplarily, a piston (33) moves along a guide member (32) to control a supply amount of the heated liquid. Any known liquid supply devices may be used as the supply unit (30) for supplying the heated liquid. It should be appreciated that the scope of the present disclosure is not limited to the configuration shown in FIG. 1.

(5) In the supply unit (30) shown in FIG. 1 which is shown non-limitedly and exemplarily, the piston (33) moves along the guide member (32) in an arrow direction to supply the liquid stored in the fluid storage (34) to the container (20) via the supply pipe (35) under the control of the controller (31).

(6) The liquid cell substance is discharged from the nozzle (12) and is laminated on the bottom of the container (20). After a first layer is laminated as shown in FIG. 1b, and then further layers are consecutively laminated thereon to form a 3D structure (40). Preferably, the heated liquid is not supplied to the container (20) before the first layer is laminated. The heated liquid may be supplied to the container (20) via the supply pipe (35) as the liquid cell substance is laminated on the first layer. It is preferable to supply the heated liquid to the container (20) at a height enough to expose the top portion of the 3D structure. FIGS. 2 and 3 are views showing a heightened 3D structure (40) and the heated liquid which is supplied at the height enough to expose the top portion of the structure.

(7) A heating unit (not shown) for maintaining the container (20) at a predetermined temperature may be provided. The heating unit may be any one of various known heat sources, such as a heating wire, a heating plate or an infrared lamp.

(8) The liquid supplied to the container 20 may be a cell culture medium.

(9) According to the present disclosure, it is very advantageous to fabricate a 3D structure since the liquid cell substance discharged from the nozzle is cured simultaneously with the lamination.

(10) While the present disclosure has been described with reference to the accompanying drawings, it should be appreciated that the scope of the present disclosure is determined by the appended claims and that the scope is not limited to the embodiments and/or the drawings. It is to be appreciated that a person skilled in the art can change or modify the embodiments without departing from the scope and spirit of the present disclosure.

(11) The present invention was supported by Priority Research Centers Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2017R1A6A1A03015562).