HYPOCRELLIN DERIVATIVE SUBSTITUTED BOTH IN A PERI-POSITION AND IN 2-POSITION BY AMINO, PREPARATION METHOD, AND APPLICATION THEREOF

Abstract

The present invention discloses a hypocrellin derivative substituted both in a peri-position and in a 2-position by an amino, and a preparation method and use thereof. A general structural formula of the derivative is as represented by formulas I-a to I-d:

##STR00001##

The hypocrellin derivative substituted both in a peri-position and in a 2-position by an amino prepared in the present invention has a maximum absorption wavelength of 600-650 nm and a molar extinction coefficient reaching about 20000-40000 M.sup.−1cm.sup.−1. Compared with unmodified hypocrellin or hypocrellin having only a 2-position modified, an absorption spectrum of the derivative is significantly red-shifted and the molar extinction coefficient is greatly improved, and the derivative can efficiently produce reactive oxygen species such as singlet oxygen in a photosensitive condition. In the same condition, the hypocrellin derivative substituted both in a peri-position and in a 2-position by an amino involved in the present invention, when used as a photosensitizer, has a stronger ability to photo-dynamically inactivate tumor cells than the first and second generation commercial photosensitizers.

Claims

1. A hypocrellin derivative substituted both in a peri-position and in a 2-position by an amino, wherein a general structural formula of the derivative is as represented by formulas I-a to I-d: ##STR00150## wherein the peri-position of hypocrellin is a 3-, 4-, 9-, or 10-position marked in formulas I-a to I-d; a substituent R.sub.3 is —COOH.sub.3 or —H; a substituent R.sub.4 is —H—F, —Cl, —Br, —I, or —S—R.sub.5, wherein R.sub.5 is a C2-12 alkyl group, a C2-12 alkyl group having a hydroxyl group as a terminal group, or a C2-12 alkyl group having a carboxyl group as a terminal group; general structural formulas of substituents R.sub.1 and R.sub.2 are respectively as represented by formula II: ##STR00151## in formula II, 0≤m≤8, 0≤n≤50, 0≤p≤8, 0≤q≤8, 0≤r≤1, and 0≤s≤8; m, n, p, q, r, and s are respectively zero or a positive integer; linking groups X and Y in formula II are respectively —NH—, —O—, —S—, a carboxylate group, an amide group, a sulfonate group, a sulfonamide group, a carbonyl group, a phosphate group, a C3-12 unsaturated hydrocarbyl group, a C3-12 cyclic hydrocarbyl group, a C6-12 aryl group, or a C3-12 heterocyclic group; the C3-12 unsaturated hydrocarbyl group is substituted or unsubstituted or heteroatom-containing alkene or alkyne; the C3-12 cyclic hydrocarbyl group is substituted or unsubstituted or heteroatom-containing cycloalkane, cycloalkene, or cycloalkyne, and the heteroatom is an oxygen, nitrogen, or sulfur atom; the C6-12 aryl group is a substituted or unsubstituted aryl group, wherein the substituted aryl group is a mono- or poly-substituted aryl group, and a substituted position is an ortho-position, a meta-position, or a para-position in the aryl group; the C3-12 heterocyclic group is a substituted or unsubstituted heterocyclic group, the substituted heterocyclic group is mono- or poly-substituted, and a substituted position is an ortho-position, a meta-position, or a para-position in a heterocycle; the heterocyclic group is furan, pyrrole, thiophene, pyrazole, imidazole, oxazole, thiazole, pyridine, piperidine, pyrimidine, pyrazine, piperazine, indole, quinoline, isoquinoline, purine, pyrimidine, or acridine; a substituent in the above cycloalkyl, cycloalkenyl, aryl, or heterocyclic group is respectively a C1-8 alkyl group, a C2-8 alkenyl group, a C2-8 alkynyl group, a C3-8 cycloalkyl group, an aryl group, a C6-12 aralkyl group, or an alkyl group having a terminal group containing a hydroxyl group, a carboxylic acid group, a sulfonic acid group, or a carboxylate group; a terminal group Z in formula II is selected from hydrogen, a C1-8 alkyl group, a C1-8 alkoxy group, a C3-8 cycloalkyl group, a phenyl group, a pyridyl group, a hydroxyl group, an amino group, a mercapto group, a carboxylic acid group, a carboxylate group, a sulfonic acid group, a sulfonate, a phosphoric acid group, a phosphate, an amino acid, triphenylphosphine, a quaternary ammonium salt, and a pyridine salt; when the terminal group Z in formula II is a quaternary ammonium salt, three substituents of the quaternary ammonium salt are respectively: a C1-8 alkyl group, a C2-8 alkenyl group, a C2-8 alkynyl group, a C3-8 cycloalkyl group, a C3-8 cycloalkenyl group, an aryl group, a C6-12 aralkyl group, or an alkyl group having a terminal group containing a hydroxyl group, a carboxylic acid group, a sulfonic acid group, or a carboxylate; and when the terminal group Z in formula II is a pyridine salt, a substituent on a pyridine ring of the pyridine salt is in an ortho-position, a meta-position, or a para-position, and the pyridine salt is obtained by quaternizing pyridine and halogenated hydrocarbons having 1 to 8 carbon atoms of different chain lengths.

2. The derivative according to claim 1, wherein the linking groups X and Y in formula II are respectively: —NH—, —O—, —S—, —COO—, —OC(═O)—, —CONH—, —NHC(═O)—, —SO.sub.3—, —SO.sub.2NH—, —C(═O)—, —PO.sub.3—, —CH═CH—, —C(CH.sub.3)═CH—, —C(CH.sub.3)═C(CH.sub.3)—, —C(COOH)═CH—, —C(CH.sub.2COOH)═CH—, —C≡C—, a cyclopropyl group, a methylcyclopropyl group, a hydroxylcyclopropyl group, a hydroxylmethylcyclopropyl group, a carboxylcyclopropyl group, a cyclobutyl group, a methylcyclobutyl group, a hydroxylcyclobutyl group, a carboxylcyclobutyl group, a cyclopentyl group, a methylcyclopentyl group, a hydroxylcyclopentyl group, a carboxylcyclopentyl group, an aminocyclopentyl group, a cyclohexyl group, a methylcyclohexyl group, an ethylcyclohexyl group, a propylcyclohexyl group, a hydroxylcyclohexyl group, an aminocyclohexyl group, a carboxylcyclohexyl group, a carboxylmethylcyclohexyl group, a dicarboxylcyclohexyl group, a cycloheptyl group, a carboxylcycloheptyl group, a hydroxylcycloheptyl group, a methylcycloheptyl group, —C.sub.6H.sub.4—, —C.sub.6H.sub.3(CH.sub.3)—, —C.sub.6H.sub.3(C.sub.2H.sub.5)—, —C.sub.6H.sub.2(CH.sub.3).sub.2—, —C.sub.6H.sub.3(OH)—, —C.sub.6H.sub.3(OCH.sub.3)—, —C.sub.6H.sub.3(OC.sub.2H.sub.5)—, —C.sub.6H.sub.3(CH.sub.2OH)—, —C.sub.6H.sub.3(NH.sub.2)—, —C.sub.6H.sub.3(CH.sub.2NH.sub.2)—, —C.sub.6H.sub.3(F)—, —C.sub.6H.sub.3(Cl)—, —C.sub.6H.sub.3(Br)—, —C.sub.6H.sub.3(I)—, —C.sub.6H.sub.3(COOH)—, —C.sub.6H.sub.2(COOH).sub.2—, —C.sub.6H.sub.3(SO.sub.3H)—, —C.sub.6H.sub.3(CH.sub.2COOH)—, —C.sub.5H.sub.3N—, —C.sub.5H.sub.2N(CH.sub.3)—, —C.sub.5H.sub.2N(OH)—, —C.sub.5H.sub.2N(NH.sub.2)—, —C.sub.5H.sub.2N(CH.sub.2NH.sub.2)—, —C.sub.5H.sub.2N(COOH)—, —C.sub.5H.sub.9N—, ##STR00152## a furan group, a pyrrolyl group, a thienyl group, a pyrazolyl group, an imidazolyl group, an oxazolyl group, a thiazolyl group, a pyridyl group, a piperidinyl group, a pyrimidinyl group, an indolyl group, a quinolinyl group, an isoquinolinyl group, a purinyl group, a pyrimidinyl group, an acridinyl group, a morpholinyl group, or a heterocyclic group containing a substituent.

3. The derivative according to claim 1, wherein the terminal group Z in formula II is: —H, —CH.sub.3, —C.sub.2H.sub.5, —C.sub.3H.sub.7, —C.sub.4H.sub.9, —C.sub.5H.sub.11, —C.sub.6H.sub.13, —C.sub.12H.sub.25, —OCH.sub.3, —OC.sub.2H.sub.5, —OC.sub.3H.sub.7, —OC.sub.4H.sub.9, —OC.sub.5H.sub.11, —OC.sub.6H.sub.13, —OC.sub.12H.sub.25, —C.sub.3H.sub.5, —C.sub.4H.sub.7, —C.sub.5H.sub.9, —C.sub.6H.sub.11, —C.sub.7H.sub.13, —C.sub.6H.sub.5, —OH, —NH.sub.2, —SH, —COOH, —COOCH.sub.3, —COOC.sub.2H.sub.5, —SO.sub.3H, —SO.sub.3CH.sub.3, —SO.sub.3C.sub.2H.sub.5, a glycine group, an alanine group, a valine group, a leucine group, an isoleucine group, a phenylalanine group, a proline group, a tryptophan group, a tyrosine group, a serine group, a cysteine group, a methionine group, an aspartate group, a glutamate group, a threonine group, a lysine group, an arginine group, a histidine group, a cystine group, a glutathione group, —PPh.sub.3.sup.+, —C.sub.5H.sub.4N.sup.+, —C.sub.5H.sub.4N.sup.+(CH.sub.3), —C.sub.5H.sub.4N.sup.+(C.sub.2H.sub.5), —C.sub.5H.sub.4N.sup.+(C.sub.12H.sub.25), —N.sup.+(CH.sub.3).sub.3, —N.sup.+(C.sub.2H.sub.5).sub.3, —N.sup.+(C.sub.3H.sub.7).sub.3, —N.sup.+(C.sub.4H.sub.9).sub.3, —N.sup.+(C.sub.6H.sub.13).sub.3, —N.sup.+(CH.sub.3).sub.2(C.sub.2H.sub.5), —N.sup.+(CH.sub.3).sub.2(C.sub.3H.sub.7), —N.sup.+(CH.sub.3).sub.2(C.sub.4H.sub.9), —N.sup.+(CH.sub.3).sub.2(C.sub.6H.sub.13), —N.sup.+(CH.sub.3).sub.2(C.sub.12H.sub.25), —N.sup.+(C.sub.2H.sub.5).sub.2(C.sub.3H.sub.7), —N.sup.+(C.sub.2H.sub.5).sub.2(C.sub.6H.sub.13), —N.sup.+(C.sub.2H.sub.5).sub.2(C.sub.12H.sub.25), or a quaternary ammonium salt having a terminal group containing a hydroxyl group, a carboxylic acid group, a sulfonic acid group, or a carboxylate.

4. The derivative according to claim 1, wherein R.sub.1 and R.sub.2 are respectively: —H, —CH.sub.3, —C.sub.2H.sub.5, —C.sub.3H.sub.7, —C.sub.4H.sub.9, —C.sub.5H.sub.11, —C.sub.6H.sub.13, —C.sub.12H.sub.25, —C.sub.6H.sub.5, —CH.sub.2C.sub.6H.sub.5, —CH.sub.2CH.sub.2C.sub.6H.sub.5, —CH.sub.2(CH.sub.2).sub.5C.sub.6H.sub.5, —C.sub.6H.sub.4(COOH), —CH.sub.2C.sub.6H.sub.4(COOH), —CH.sub.2C.sub.6H.sub.4(OH), —C.sub.6H.sub.4(CH.sub.2COOH), —CH.sub.2C.sub.6H.sub.4(CH.sub.2COOH), a cyclopropyl group, a methylcyclopropyl group, a hydroxylcyclopropyl group, a hydroxylmethylcyclopropyl group, a carboxylcyclopropyl group, a cyclobutyl group, a methylcyclobutyl group, a hydroxylcyclobutyl group, a carboxylcyclobutyl group, —CH.sub.2C.sub.4H.sub.6(COOH), a cyclopentyl group, a methylcyclopentyl group, a hydroxylcyclopentyl group, an aminocyclopentyl group, a carboxylcyclopentyl group, a cyclohexyl group, a methylcyclohexyl group, an ethylcyclohexyl group, a propylcyclohexyl group, a hydroxylcyclohexyl group, an aminocyclohexyl group, a carboxylcyclohexyl group, a carboxylmethylcyclohexyl group, a dicarboxylcyclohexyl group, —CH.sub.2C.sub.6H.sub.10(COOH), —CH.sub.2C.sub.6H.sub.10(OH), a cycloheptyl group, a carboxylcycloheptyl group, a hydroxylcycloheptyl group, a methylcycloheptyl group, —CH.sub.2COOH, —CH.sub.2CH.sub.2COOH, —CH.sub.2(CH.sub.2).sub.2COOH, —CH.sub.2(CH.sub.2).sub.3COOH, —CH.sub.2(CH.sub.2).sub.4COOH, —CH.sub.2(CH.sub.2).sub.5COOH, —CH.sub.2(CH.sub.2).sub.6COOH, —CH.sub.2(CH.sub.2).sub.10COOH, —CH.sub.2COOCH.sub.3, —CH.sub.2CH.sub.2COOC.sub.6H.sub.13, —CH.sub.2(CH.sub.2).sub.2COOCH.sub.3, —CH.sub.2(CH.sub.2).sub.2COOC.sub.2H.sub.5, —CH.sub.2(CH.sub.2).sub.2COOC.sub.6H.sub.13, —CH.sub.2(CH.sub.2).sub.4COOCH.sub.3, —CH.sub.2(CH.sub.2).sub.6COOC.sub.6H.sub.13, —CH.sub.2COONa.sup.+, —CH.sub.2(CH.sub.2).sub.2COONa.sup.+, —CH.sub.2(CH.sub.2).sub.4COONa.sup.+, —CH.sub.2SO.sub.3H, —CH.sub.2CH.sub.2SO.sub.3H, —CH.sub.2(CH.sub.2).sub.2SO.sub.3H, —CH.sub.2(CH.sub.2).sub.3SO.sub.3H, —CH.sub.2(CH.sub.2).sub.4SO.sub.3H, —CH.sub.2(CH.sub.2).sub.5SO.sub.3H, —CH.sub.2(CH.sub.2).sub.11SO.sub.3H, —CH.sub.2SO.sub.3CH.sub.3, —CH.sub.2SO.sub.3C.sub.6H.sub.13, —CH.sub.2CH.sub.2SO.sub.3CH.sub.3, —CH.sub.2(CH.sub.2).sub.2SO.sub.3CH.sub.3, —CH.sub.2(CH.sub.2).sub.2SO.sub.3C.sub.6H.sub.13, —CH.sub.2(CH.sub.2).sub.4SO.sub.3C.sub.4H.sub.9, —CH.sub.2(CH.sub.2).sub.11SO.sub.3C.sub.6H.sub.13, —CH.sub.2SO.sub.3Na, —CH.sub.2CH.sub.2SO.sub.3K, —OH, —OCH.sub.3, —OC.sub.2H.sub.5, —OC.sub.6H.sub.13, —NH.sub.2, —NHC.sub.2H.sub.5, —NHC.sub.6H.sub.13, —NHC.sub.12H.sub.25, —NHC.sub.6H.sub.5, —NHC.sub.5H.sub.4N, —C.sub.5H.sub.4N, —CH.sub.2C.sub.5H.sub.4N, —(CH.sub.2).sub.2C.sub.5H.sub.4N, —(CH.sub.2).sub.6C.sub.5H.sub.4N, —C.sub.5H.sub.3N(CH.sub.3), —C.sub.5H.sub.3N(OH), —C.sub.5H.sub.3N(NH.sub.2), —C.sub.5H.sub.3N(COOH), —C.sub.5H.sub.3N(CH.sub.2COOH), —CH.sub.2C.sub.5H.sub.3N(CH.sub.2COOH), —CH.sub.2CH.sub.2—(OCH.sub.2CH.sub.2).sub.n—OH, —CH.sub.2CH.sub.2—(OCH.sub.2CH.sub.2).sub.n—OCH.sub.3, —CH.sub.2CH.sub.2—(OCH.sub.2CH.sub.2).sub.n—OC.sub.6H.sub.13, —CH.sub.2CH.sub.2—(OCH.sub.2CH.sub.2).sub.n—OC.sub.12H.sub.25, —CH.sub.2CH.sub.2—(OCH.sub.2CH.sub.2).sub.n—O—COCH.sub.3, —CH.sub.2CH.sub.2—(OCH.sub.2CH.sub.2).sub.n—O—COC.sub.6H.sub.13, —CH.sub.2CH.sub.2—O—CO—CH.sub.2CH.sub.2—(OCH.sub.2CH.sub.2).sub.n—OH, —CH.sub.2CH.sub.2—O—CO—CH.sub.2CH.sub.2—(OCH.sub.2CH.sub.2).sub.n—OCH.sub.3, —CH.sub.2CH.sub.2—OCH.sub.2CH.sub.2—O—CO—CH.sub.2CH.sub.2—(OCH.sub.2CH.sub.2).sub.n—OH, —CH.sub.2CH.sub.2—OCH.sub.2CH.sub.2—O—CO—CH.sub.2CH.sub.2—(OCH.sub.2CH.sub.2).sub.n—OCH.sub.3, —CH.sub.2CH.sub.2—OCH.sub.2CH.sub.2—OCH.sub.2CH.sub.2—O—CO—CH.sub.2CH.sub.2—(OCH.sub.2CH.sub.2).sub.n—OH, —CH.sub.2CH.sub.2—OCH.sub.2CH.sub.2—OCH.sub.2CH.sub.2—O—CO—CH.sub.2CH.sub.2—(OCH.sub.2CH.sub.2).sub.n—OCH.sub.3, —CH.sub.2CH.sub.2—O—CO—CH.sub.2CH.sub.2—PPh.sub.3.sup.+, —CH.sub.2CH.sub.2—O—CO—(CH.sub.2).sub.3—PPh.sub.3.sup.+, —CH.sub.2CH.sub.2—O—CO—(CH.sub.2).sub.5—PPh.sub.3.sup.+, —CH.sub.2CH.sub.2—OCH.sub.2CH.sub.2—O—CO—CH.sub.2CH.sub.2—PPh.sub.3.sup.+, —CH.sub.2CH.sub.2—OCH.sub.2CH.sub.2—O—CO—(CH.sub.2).sub.3—PPh.sub.3.sup.+, —CH.sub.2CH.sub.2—OCH.sub.2CH.sub.2—O—CO—(CH.sub.2).sub.5—PPh.sub.3.sup.+; —(CH.sub.2).sub.3—OH, —(CH.sub.2).sub.3—OCH.sub.3, —(CH.sub.2).sub.3—OC.sub.2H.sub.5, —(CH.sub.2).sub.3—OCOCH.sub.3, —(CH.sub.2).sub.3—OCOC.sub.2H.sub.5, —(CH.sub.2).sub.3—O—COCH.sub.2CH.sub.2—(OCH.sub.2CH.sub.2).sub.n—OCH.sub.3, —(CH.sub.2).sub.4—OH, —(CH.sub.2).sub.4—OCH.sub.3, —(CH.sub.2).sub.4—OCOCH.sub.3, —(CH.sub.2).sub.4—OCOC.sub.2H.sub.5, —(CH.sub.2).sub.4—O—COCH.sub.2CH.sub.2—(OCH.sub.2CH.sub.2).sub.n—OCH.sub.3, —(CH.sub.2).sub.6—OH, —(CH.sub.2).sub.6—OCH.sub.3, —(CH.sub.2).sub.6—OCOCH.sub.3, —(CH.sub.2).sub.6—O—COCH.sub.2CH.sub.2—(OCH.sub.2CH.sub.2).sub.n—OCH.sub.3; —CH.sub.2CH.sub.2—NH—CH.sub.2CH.sub.2—(OCH.sub.2CH.sub.2).sub.n—OH, —CH.sub.2CH.sub.2—NH—CH.sub.2CH.sub.2—(OCH.sub.2CH.sub.2).sub.n—OCH.sub.3, —CH.sub.2CH.sub.2—(NHCH.sub.2CH.sub.2).sub.n—NH.sub.2, —CH.sub.2CH.sub.2—(NHCH.sub.2CH.sub.2).sub.n—N(CH.sub.3).sub.2, —CH.sub.2CH.sub.2—NHCH.sub.2CH.sub.2—NH—COCH.sub.2CH.sub.2—(OCH.sub.2CH.sub.2).sub.n—OCH.sub.3, —CH.sub.2CH.sub.2—S—CH.sub.2CH.sub.2—(OCH.sub.2CH.sub.2).sub.n—OH, —CH(CH.sub.3)—COOH, —CH(CH(CH.sub.3).sub.2)—COOH, —CHCH.sub.2(CH(CH.sub.3).sub.2)—COOH, —CH(CH.sub.2CH.sub.2SCH.sub.3)—COOH, —CHCH(CH.sub.3)(C.sub.2H.sub.5)—COOH, —CH(CH.sub.2OH)—COOH, —CHCH(OH)(CH.sub.3)—COOH, —CH(CH.sub.2SH)—COOH, —CH(CH.sub.2CONH.sub.2)—COOH, —CH(CH.sub.2CH.sub.2CONH.sub.2)—COOH, —CH(CH.sub.2C.sub.6H.sub.5)—COOH, —CH(CH.sub.2C.sub.6H.sub.5OH)—COOH, —CH(CH.sub.2CH.sub.2CH.sub.2CH.sub.2NH.sub.3.sup.+)—COOH, —CH(COOH)—CH.sub.2COOH, —CH(COOH)—CH.sub.2CH.sub.2COOH, ##STR00153## —CH(CH.sub.3)—COOCH.sub.3, —CH(CH(CH.sub.3).sub.2)—COOCH.sub.3, —CHCH.sub.2(CH(CH.sub.3).sub.2)—COOCH.sub.3, —CH(CH.sub.2CH.sub.2SCH.sub.3)—COOCH.sub.3, —CH(CH.sub.3)—COONa.sup.+, —CH(CH(CH.sub.3).sub.2)—COONa.sup.+, —CHCH.sub.2(CH(CH.sub.3).sub.2)—COOK.sup.+, —CH(CH.sub.2CH.sub.2SCH.sub.3)—COOK.sup.+; —CH.sub.2CO—(OCH.sub.2CH.sub.2).sub.n—OH, —CH.sub.2CO—(OCH.sub.2CH.sub.2).sub.n—OCH.sub.3, —CH.sub.2CH.sub.2CO—(OCH.sub.2CH.sub.2).sub.n—OH, —CH.sub.2CH.sub.2CO—(OCH.sub.2CH.sub.2).sub.n—OCH.sub.3, —CH.sub.2(CH.sub.2).sub.2CO—(OCH.sub.2CH.sub.2).sub.n—OH, —CH.sub.2(CH.sub.2).sub.2CO—(OCH.sub.2CH.sub.2).sub.n—OCH.sub.3, —CH.sub.2(CH.sub.2).sub.4CO—(OCH.sub.2CH.sub.2).sub.n—OH, —CH.sub.2(CH.sub.2).sub.4CO—(OCH.sub.2CH.sub.2).sub.n—OCH.sub.3, —CH.sub.2—CO—NH—CH.sub.2CH.sub.2—(OCH.sub.2CH.sub.2).sub.n—OH, —CH.sub.2—CO—NH—CH.sub.2CH.sub.2—(OCH.sub.2CH.sub.2).sub.n—OCH.sub.3, —(CH.sub.2).sub.2—CO—NH—CH.sub.2CH.sub.2—(OCH.sub.2CH.sub.2).sub.n—OH, —(CH.sub.2).sub.2—CO—NH—CH.sub.2CH.sub.2—(OCH.sub.2CH.sub.2).sub.n—OCH.sub.3, —(CH.sub.2).sub.3—CO—NH—CH.sub.2CH.sub.2—(OCH.sub.2CH.sub.2).sub.n—OH, —(CH.sub.2).sub.3—CO—NH—CH.sub.2CH.sub.2—(OCH.sub.2CH.sub.2).sub.n—OCH.sub.3, —(CH.sub.2).sub.4—CO—NH—CH.sub.2CH.sub.2—(OCH.sub.2CH.sub.2).sub.n—OH, —(CH.sub.2).sub.4—CO—NH—CH.sub.2CH.sub.2—(OCH.sub.2CH.sub.2).sub.n—OCH.sub.3, —(CH.sub.2).sub.5—CO—NH—CH.sub.2CH.sub.2—(OCH.sub.2CH.sub.2).sub.n—OH, —(CH.sub.2).sub.5—CO—NH—CH.sub.2CH.sub.2—(OCH.sub.2CH.sub.2).sub.n—OCH.sub.3, —CH.sub.2—SO.sub.2—(OCH.sub.2CH.sub.2).sub.n—OH, —CH.sub.2—SO.sub.2—(OCH.sub.2CH.sub.2).sub.n—OCH.sub.3, —(CH.sub.2).sub.2—SO.sub.2—(OCH.sub.2CH.sub.2).sub.n—OH, —(CH.sub.2).sub.2—SO.sub.2—(OCH.sub.2CH.sub.2).sub.n—OCH.sub.3, —(CH.sub.2).sub.3—SO.sub.2—(OCH.sub.2CH.sub.2).sub.n—OH, —(CH.sub.2).sub.3—SO.sub.2—(OCH.sub.2CH.sub.2).sub.n—OCH.sub.3, —(CH.sub.2).sub.4—SO.sub.2—(OCH.sub.2CH.sub.2).sub.n—OH, —(CH.sub.2).sub.4—SO.sub.2—(OCH.sub.2CH.sub.2).sub.n—OCH.sub.3, —(CH.sub.2).sub.5—SO.sub.2—(OCH.sub.2CH.sub.2).sub.n—OH, —(CH.sub.2).sub.5—SO.sub.2—(OCH.sub.2CH.sub.2).sub.n—OCH.sub.3, —(CH.sub.2).sub.6—SO.sub.2—(OCH.sub.2CH.sub.2).sub.n—OH, —(CH.sub.2).sub.6—SO.sub.2—(OCH.sub.2CH.sub.2).sub.n—OCH.sub.3, —CH.sub.2—SO.sub.2—NHCH.sub.2CH.sub.2—(OCH.sub.2CH.sub.2).sub.n—OH, —CH.sub.2—SO.sub.2—NHCH.sub.2CH.sub.2—(OCH.sub.2CH.sub.2).sub.n—OCH.sub.3, —(CH.sub.2).sub.2—SO.sub.2—NHCH.sub.2CH.sub.2—(OCH.sub.2CH.sub.2).sub.n—OH, —(CH.sub.2).sub.2—SO.sub.2—NHCH.sub.2CH.sub.2—(OCH.sub.2CH.sub.2).sub.n—OCH.sub.3, —(CH.sub.2).sub.3—SO.sub.2—NHCH.sub.2CH.sub.2—(OCH.sub.2CH.sub.2).sub.n—OCH.sub.3, —(CH.sub.2).sub.4—SO.sub.2—NHCH.sub.2CH.sub.2—(OCH.sub.2CH.sub.2).sub.n—OCH.sub.3, —(CH.sub.2).sub.5—SO.sub.2—NHCH.sub.2CH.sub.2—(OCH.sub.2CH.sub.2).sub.n—OCH.sub.3, —(CH.sub.2).sub.6—SO.sub.2—NHCH.sub.2CH.sub.2—(OCH.sub.2CH.sub.2).sub.n—OCH.sub.3, —CH.sub.2CH.sub.2—N.sup.+(CH.sub.3).sub.3, —(CH.sub.2).sub.3—N.sup.+(CH.sub.3).sub.3, —(CH.sub.2).sub.4—N.sup.+(CH.sub.3).sub.3, —(CH.sub.2).sub.5—N.sup.+(CH.sub.3).sub.3, —(CH.sub.2).sub.6—N.sup.+(CH.sub.3).sub.3, —(CH.sub.2).sub.12—N.sup.+(CH.sub.3).sub.3, —CH.sub.2CH.sub.2—N.sup.+(C.sub.2H.sub.5).sub.3, —(CH.sub.2).sub.4—N.sup.+(C.sub.2H.sub.5).sub.3, —(CH.sub.2).sub.6—N.sup.+(C.sub.2H.sub.5).sub.3, —(CH.sub.2).sub.12—N.sup.+(C.sub.2H.sub.5).sub.3, —CH.sub.2CH.sub.2—N.sup.+(C.sub.3H.sub.7).sub.3, (CH.sub.2).sub.4—N.sup.+(C.sub.3H.sub.7).sub.3, —(CH.sub.2).sub.6—N.sup.+(C.sub.3H.sub.7).sub.3, —CH.sub.2CH.sub.2—N.sup.+(C.sub.4H.sub.9).sub.3, —(CH.sub.2).sub.6—N.sup.+(C.sub.4H.sub.9).sub.3, —CH.sub.2CH.sub.2—N.sup.+(CH.sub.3).sub.2(C.sub.2H.sub.5), —CH.sub.2CH.sub.2—N.sup.+(CH.sub.3).sub.2(C.sub.4H.sub.9), —CH.sub.2CH.sub.2—N.sup.+(CH.sub.3).sub.2(C.sub.6H.sub.13), —CH.sub.2CH.sub.2—N.sup.+(CH.sub.3).sub.2(C.sub.12H.sub.25), —(CH.sub.2).sub.3—N.sup.+(CH.sub.3).sub.2(C.sub.4H.sub.9), —(CH.sub.2).sub.3—N.sup.+(CH.sub.3).sub.2(C.sub.6H.sub.13), —(CH.sub.2).sub.3—N.sup.+(CH.sub.3).sub.2(C.sub.12H.sub.25), —(CH.sub.2).sub.4—N.sup.+(CH.sub.3).sub.2(C.sub.6H.sub.13), —(CH.sub.2).sub.4—N.sup.+(CH.sub.3).sub.2(C.sub.12H.sub.25), —(CH.sub.2).sub.5—N.sup.+(CH.sub.3).sub.2(C.sub.2H.sub.5), —(CH.sub.2).sub.5—N.sup.+(CH.sub.3).sub.2(C.sub.6H.sub.13), —(CH.sub.2).sub.5—N.sup.+(CH.sub.3).sub.2(C.sub.12H.sub.25), —(CH.sub.2).sub.6—N.sup.+(CH.sub.3).sub.2(C.sub.2H.sub.5), —(CH.sub.2).sub.6—N.sup.+(CH.sub.3).sub.2(C.sub.6H.sub.13), —(CH.sub.2).sub.6—N.sup.+(CH.sub.3).sub.2(C.sub.12H.sub.25); —CH.sub.2CO—OCH.sub.2CH.sub.2—N.sup.+(CH.sub.3).sub.3, —CH.sub.2CH.sub.2CO—OCH.sub.2CH.sub.2—N.sup.+(CH.sub.3).sub.3, —CH.sub.2(CH.sub.2).sub.2CO—OCH.sub.2CH.sub.2—N.sup.+(CH.sub.3).sub.3, —CH.sub.2(CH.sub.2).sub.6CO—OCH.sub.2CH.sub.2—N.sup.+(CH.sub.3).sub.3, —CH.sub.2CO—O—(CH.sub.3).sub.3—N.sup.+(CH.sub.3).sub.3, —CH.sub.2(CH.sub.2).sub.2CO—O—(CH.sub.3).sub.3—N.sup.+(CH.sub.3).sub.3, —CH.sub.2COOCH.sub.2CH.sub.2—N.sup.+(CH.sub.3).sub.2(C.sub.6H.sub.13); —CH.sub.2CONH—CH.sub.2CH.sub.2—N.sup.+(CH.sub.3).sub.3, —CH.sub.2CH.sub.2CONH—CH.sub.2CH.sub.2—N.sup.+(CH.sub.3).sub.3, —CH.sub.2(CH.sub.2).sub.4CONH—CH.sub.2CH.sub.2—N.sup.+(CH.sub.3).sub.3, —CH.sub.2CONH—(CH.sub.2).sub.3—N.sup.+(CH.sub.3).sub.3, —CH.sub.2CH.sub.2CONH—(CH.sub.2).sub.3—N.sup.+(CH.sub.3).sub.3, —CH.sub.2(CH.sub.2).sub.4CONH—(CH.sub.2).sub.3—N.sup.+(CH.sub.3).sub.3, —CH.sub.2CONH—(CH.sub.2).sub.4—N.sup.+(CH.sub.3).sub.3, —CH.sub.2CH.sub.2CONH—(CH.sub.2).sub.4—N.sup.+(CH.sub.3).sub.3, —CH.sub.2(CH.sub.2).sub.4CONH—(CH.sub.2).sub.4—N.sup.+(CH.sub.3).sub.3, —CH.sub.2CONH—(CH.sub.2).sub.5—N.sup.+(CH.sub.3).sub.3, —CH.sub.2CH.sub.2CONH—(CH.sub.2).sub.5—N.sup.+(CH.sub.3).sub.3, —CH.sub.2(CH.sub.2).sub.4CONH—(CH.sub.2).sub.5—N.sup.+(CH.sub.3).sub.3, —CH.sub.2CONH—(CH.sub.2).sub.6—N.sup.+(CH.sub.3).sub.3, —CH.sub.2CH.sub.2CONH—(CH.sub.2).sub.6—N.sup.+(CH.sub.3).sub.3, —CH.sub.2(CH.sub.2).sub.4CONH—(CH.sub.2).sub.6—N.sup.+(CH.sub.3).sub.3, —CH.sub.2CONH—CH.sub.2CH.sub.2—N.sup.+(CH.sub.3).sub.2(C.sub.6H.sub.13), —CH.sub.2CONH—CH.sub.2CH.sub.2—N.sup.+(CH.sub.3).sub.2(C.sub.12H.sub.25); —C.sub.5H.sub.4N.sup.+(CH.sub.3), —CH.sub.2C.sub.5H.sub.4N.sup.+(CH.sub.3), —CH.sub.2C.sub.5H.sub.4N.sup.+(C.sub.6H.sub.13), —CH.sub.2C.sub.5H.sub.4N.sup.+(CH.sub.2COOH), —CH.sub.2CH.sub.2C.sub.5H.sub.4N.sup.+(CH.sub.3), —CH.sub.2CH.sub.2C.sub.5H.sub.4N.sup.+(C.sub.6H.sub.13), —CH.sub.2CH.sub.2C.sub.5H.sub.4N.sup.+(CH.sub.2COOH), ##STR00154## ##STR00155## ##STR00156## ##STR00157## ##STR00158## ##STR00159## or a heterocyclic group containing a substituent; and wherein n is a positive integer between 0 and 50.

5. The derivative according to claim 1, wherein the derivative has an enol tautomer; and wherein formula I-a and formula I-a′ represent enol tautomers regarding positions 9 and 10 in the structural formula; formula I-b and formula I-b′ represent enol tautomers regarding positions 3 and 4 in the structural formula; formula I-c and formula I-c′ represent enol tautomers regarding positions 9 and 10 in the structural formula; and formula I-d and formula I-d′ represent enol tautomers regarding positions 3 and 4 in the structural formula: ##STR00160##

6. A method for preparing the derivative according to claim 1, wherein the method comprises the following steps: mixing a hypocrellin raw material and a corresponding amino substituted derivative in a solvent, reacting for 4-24 hours under the protection of a protective gas in a lucifugous condition, with a reaction temperature of 20-150° C., and performing separation and purification for a product, to obtain a hypocrellin derivative substituted both in a peri-position and in a 2-position by an amino.

7. The method according to claim 6, wherein the hypocrellin raw material is hypocrellin B or deacetyl hypocrellin; a general structural formula of the amino substituted derivative is R.sub.1—NH.sub.2 or R.sub.2—NH.sub.2; a feeding molar ratio of the hypocrellin raw material and the corresponding amino substituted derivative is 1:10-1:100; the solvent is an organic solvent, or a mixed solvent of an organic solvent and water; and the organic solvent is a mixture of one or more of dichloromethane, acetonitrile, tetrahydrofuran, pyridine, methanol, ethanol, and water.

8. A use of the derivative according to claim 1 as a photosensitizer drug in photodynamic therapy.

9. A method for preparing the derivative according to claim 2, wherein the method comprises the following steps: mixing a hypocrellin raw material and a corresponding amino substituted derivative in a solvent, reacting for 4-24 hours under the protection of a protective gas in a lucifugous condition, with a reaction temperature of 20-150° C., and performing separation and purification for a product, to obtain a hypocrellin derivative substituted both in a peri-position and in a 2-position by an amino.

10. A method for preparing the derivative according to claim 3, wherein the method comprises the following steps: mixing a hypocrellin raw material and a corresponding amino substituted derivative in a solvent, reacting for 4-24 hours under the protection of a protective gas in a lucifugous condition, with a reaction temperature of 20-150° C., and performing separation and purification for a product, to obtain a hypocrellin derivative substituted both in a peri-position and in a 2-position by an amino.

11. A method for preparing the derivative according to claim 4, wherein the method comprises the following steps: mixing a hypocrellin raw material and a corresponding amino substituted derivative in a solvent, reacting for 4-24 hours under the protection of a protective gas in a lucifugous condition, with a reaction temperature of 20-150° C., and performing separation and purification for a product, to obtain a hypocrellin derivative substituted both in a peri-position and in a 2-position by an amino.

12. A method for preparing the derivative according to claim 5, wherein the method comprises the following steps: mixing a hypocrellin raw material and a corresponding amino substituted derivative in a solvent, reacting for 4-24 hours under the protection of a protective gas in a lucifugous condition, with a reaction temperature of 20-150° C., and performing separation and purification for a product, to obtain a hypocrellin derivative substituted both in a peri-position and in a 2-position by an amino.

13. A use of the derivative according to claim 2 as a photosensitizer drug in photodynamic therapy.

14. A use of the derivative according to claim 3 as a photosensitizer drug in photodynamic therapy.

15. A use of the derivative according to claim 4 as a photosensitizer drug in photodynamic therapy.

16. A use of the derivative according to claim 5 as a photosensitizer drug in photodynamic therapy.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0093] The specific embodiments of the present invention will be further described in detail below with reference to the accompanying drawings.

[0094] FIG. 1 shows a general structural formula of a hypocrellin derivative substituted both in a peri-position and in a 2-position by an amino of the present invention;

[0095] FIG. 2 shows a diagram of a synthetic route of hypocrellin B derivatives HB-1a-PEGn-HB-d-PEGn having a peri-position and a 2-position both substituted by a polyethylene glycol-2-(2-aminoethoxy)ethanol group in examples 2 and 3 (n is the number of units of the polyethylene glycol);

[0096] FIG. 3 shows a diagram of a synthetic route of deacetyl hypocrellin derivatives HC-1a-PEGn-HC-d-PEGn having a peri-position and a 2-position both substituted by a polyethylene glycol-2-(2-aminoethoxy)ethanol group in examples 5 and 6 (n is the number of units of the polyethylene glycol);

[0097] FIG. 4 shows a diagram of a synthetic route of deacetyl hypocrellin derivatives HC-8a-PEGn-HC-8d-PEGn having a peri-position and a 2-position both substituted by a polyethylene glycol-aminobutyric acid group in examples 22 and 23 (n is the number of units of the polyethylene glycol);

[0098] FIG. 5(a) shows a comparison diagram of absorption spectra of hypocrellin and the derivative thereof, in which line a represents hypocrellin B HB extracted in example 1 of the present invention; line b represents a di-2-(2-aminoethoxy)ethanol-substituted hypocrellin B derivative HB-1c prepared in example 2; and line c represents a di-2-(2-aminoethoxy)ethanol-substituted deacetyl hypocrellin derivative HC-1c prepared in example 5;

[0099] FIG. 5(b) shows a comparison diagram of absorption spectra of a commercial porphyrin photosensitizer PpIX and a porphyrin photosensitizer Ce6, respectively;

[0100] FIG. 6(a) shows a function diagram of the di-(2-aminoethoxy)ethanol-substituted hypocrellin B derivative HB-1c in example 2 of the present invention and a singlet oxygen scavenger:

[0101] FIG. 6(b) shows a function diagram of the di-2-(2-aminoethoxy)ethanol-substituted hypocrellin B derivative HB-1c in example 2 of the present invention and a superoxide radical scavenger;

[0102] FIGS. 7(a)-7(d) show a singlet oxygen efficiency test;

[0103] FIG. 7(a) shows a photodegradation curve of the di-2-(2-aminoethoxy)ethanol-substituted hypocrellin B derivative HB-1c in example 2 of the present invention;

[0104] FIG. 7(b) shows a photodegradation curve of the di-2-(2-aminoethoxy)ethanol-substituted deacetyl hypocrellin derivative HC-1c in example 5 of the present invention;

[0105] FIG. 7(c) shows a photodegradation curve of a standard reference rose bengal (RB);

[0106] FIG. 7(d) is a comparison diagram of photodegradation of HB-1c, HC-1c, and RB;

[0107] FIG. 8 shows a comparison diagram of pH stabilities of the hypocrellin derivative prepared in the present invention and commercial hematoporphyrin, in which curve (a) shows the pH stability of the commercial hematoporphyrin photosensitizer HpD: curve (b) shows the pH stability of the di-2-(2-aminoethoxy)ethanol-substituted deacetyl hypocrellin derivative HC-1c in example 5; and curve (c) shows the pH stability of polyethylene glycol-di-2-(2-aminoethoxy)ethanol-substituted deacetyl hypocrellin derivative HC-1c-PEG6 in example 6;

[0108] FIG. 9 shows a comparison diagram of photostabilities of the hypocrellin derivative prepared in the present invention and a commercial photosensitizer under irradiation with a laser for 30 min at a light intensity of 20 mW/cm.sup.2, in which curve (a) shows the photostability of a diaminoethanol-substituted deacetyl hypocrellin B derivative HC-2c in example 9; curve (b) shows the photostability of a polyethylene glycol-diaminoethanol-substituted deacetyl hypocrellin B derivative HC-2c-PEG6 in example 10; curve (c) shows the photostability of a commercial porphin photosensitizer Ce6; and curve (d) shows the photostability of the commercial hematoporphyrin photosensitizer HpD;

[0109] FIGS. 10(a)-10(c) show confocal fluorescence images in Hela cells of the di-2-(2-aminoethoxy)ethanol-substituted hypocrellin B derivative HB-1c in example 2 of the present invention;

[0110] FIG. 10(a) shows a dark field image;

[0111] FIG. 10(b) shows alight field image;

[0112] FIG. 10(c) shows an image of superimposed dark field and bright field;

[0113] FIG. 11 shows detection of reactive oxygen in a cell for photosensitizer incubation by using a singlet oxygen fluorescence probe DCFH-DA, wherein the first row shows fluorescence images of a cell for incubation of only the photosensitizer HC-1c-PEG6 at different times: the second row shows fluorescence images of a cell for incubation of only the singlet oxygen fluorescence probe DCFH-DA at different times; and the third row shows fluorescence images of a cell for co-incubation of the photosensitizer HC-c-PEG6 and fluorescence probe DCFH-DA at different times;

[0114] FIG. 12(a) shows dark toxicities, to Hela cells, of the hematoporphyrin derivative HpD, the hypocrellin B HB, and the polyethylene glycol-di-2-(2-aminoethoxy)ethanol-substituted hypocrellin derivative HB-c-PEG6 in example 3 of the present invention at different concentrations;

FIG. 12(b) shows phototoxicities, to Hela cells, of the hematoporphyrin derivative HpD, the hypocrellin B HB, and the polyethylene glycol-di-2-(2-aminoethoxy)ethanol-substituted hypocrellin derivative HB-c-PEG6 in example 3 of the present invention at different concentrations;

[0115] FIG. 13(a) shows dark toxicities, to Hela cells, of the hematoporphyrin derivative HpD, the deacetyl hypocrellin HC, and the polyethylene glycol-di-2-(2-aminoethoxy)ethanol-substituted deacetyl hypocrellin derivative HC-1c-PEG6 in example 6 of the present invention at different concentrations;

[0116] FIG. 13(b) shows phototoxicities, to Hela cells, of the hematoporphyrin derivative HpD, the deacetyl hypocrellin HC, and the polyethylene glycol-di-2-(2-aminoethoxy)ethanol-substituted deacetyl hypocrellin derivative HC-1c-PEG6 in example 6 of the present invention at different concentrations;

[0117] FIG. 14(a) shows dark toxicities, to Hela cells, of the hematoporphyrin derivative HpD, the deacetyl hypocrellin HC, and the diaminobutyric acid-substituted deacetyl hypocrellin HC-8c synthesized in example 22 of the present invention at different concentrations;

[0118] FIG. 14(b) shows phototoxicities, to Hela cells, of the hematoporphyrin derivative HpD, the deacetyl hypocrellin HC, and the diaminobutyric acid-substituted deacetyl hypocrellin HC-8c synthesized in example 22 of the present invention at different concentrations;

[0119] FIG. 15(a) shows dark toxicities, to Hela cells, of the hematoporphyrin derivative HpD, the deacetyl hypocrellin HC, and the polyethylene glycol-diaminobutyric acid-modified deacetyl hypocrellin HC-8c-PEG6 synthesized in example 23 of the present invention at different concentrations;

[0120] FIG. 15(b) shows phototoxicities, to Hela cells, of the hematoporphyrin derivative HpD, the deacetyl hypocrellin HC, and the polyethylene glycol-diaminobutyric acid-modified deacetyl hypocrellin HC-8c-PEG6 synthesized in example 23 of the present invention at different concentrations;

[0121] FIG. 16(a) shows dark toxicities, to Hela cells, of the hematoporphyrin derivative HpD, the hypocrellin B HB, and the 4-aminomethylcyclohexanoic acid-substituted hypocrellin B derivative HB-13c synthesized in example 35 of the present invention at different concentrations;

[0122] FIG. 16(b) shows phototoxicities, to Hela cells, of the hematoporphyrin derivative HpD, the hypocrellin B HB, and the 4-aminomethylcyclohexanoic acid-substituted hypocrellin B derivative HB-13c synthesized in example 35 of the present invention at different concentrations;

[0123] FIG. 17(a) shows dark toxicities, to Hela cells, of the hematoporphyrin derivative HpD, the hypocrellin B HB, and HB-13c-PEG8 synthesized in example 37 of the present invention at different concentrations, wherein HB-13c-PEG8 is obtained by connecting the 4-aminomethylcyclohexanoic acid-substituted hypocrellin B derivative and two 8-PEG chains with a carboxylic acid ester bond;

[0124] FIG. 17(b) shows phototoxicities, to Hela cells, of the hematoporphyrin derivative HpD, the hypocrellin B HB, and HB-13c-PEG8 synthesized in example 37 of the present invention at different concentrations, wherein HB-13c-PEG8 is obtained by connecting the 4-aminomethylcyclohexanoic acid-substituted hypocrellin B derivative and two 8-PEG chains with a carboxylic acid ester bond;

[0125] FIG. 18(a) shows dark toxicities, to Hela cells, of the hematoporphyrin derivative HpD, the hypocrellin B HB, and HC-13c-NH-PEG8 synthesized in example 41 of the present invention at different concentrations, wherein HC-13c-NH-PEG8 is obtained by connecting the 4-aminomethylcyclohexanoic acid-substituted hypocrellin B derivative and two 8-PEG chains with an amide bond; and

[0126] FIG. 18(b) shows phototoxicities, to Hela cells, of the hematoporphyrin derivative HpD, the hypocrellin B HB, and HC-13c-NH-PEG8 synthesized in example 41 of the present invention at different concentrations, wherein HC-13c-NH-PEG8 is obtained by connecting the 4-aminomethylcyclohexanoic acid-substituted hypocrellin B derivative and two 8-PEG chains with an amide bond;

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0127] In order to describe the present invention more clearly, the present invention is further described below with reference to the preferred embodiments and the accompanying drawings. Those skilled in the art should understand that the contents specifically described below is for illustration, instead of limitation, and the protection scope of the present invention shall not be limited thereto.

[0128] In the present invention, the experimental methods are conventional methods unless otherwise specified. Unless otherwise specified, the raw materials used can be obtained from publicly available commercial channels; the percentages are mass percentages unless otherwise specified; and M represents mol/L unless otherwise specified.

Example 1

[0129] Extraction of hypocrellin A (HA): 100 g of Hypocrella bambusae was pulverized by suing a pulverizer and placed in a Soxhlet extractor, continuous extraction was performed for a day with 1000 mL of acetone as a solvent until an extracting solution was nearly colorless, the extracting solution was filtered to remove a small amount of infiltrated insoluble solid and then spin-dried to remove acetone, 500 mL of dichloromethane was used for dissolution, 3×400 mL of distilled water was used for washing, an organic layer was separated out and spin-dried, a solid residue was washed with 3×100 mL of petroleum ether, the solid was spontaneously combusted and air-dried in air and then recrystallized twice by using chloroform-petroleum ether, and an obtained crystal was the target product hypocrellin A (HA), with a purity of above 98%, and MS (ESI+): 546.8. Further purification can be performed by means of thin-layer silica gel plate chromatography using petroleum ether:ethyl acetate:anhydrous ethanol (30:10:1) as a developing agent, to obtain hypocrellin A with a higher purity.

[0130] Preparation of hypocrellin B (HB): hypocrellin B was obtained by dehydrating hypocrellin A in an alkaline condition, and for a preparation method, reference is made to Zhao Kaihong, 1989, Organic Chemistry, volume 9, pages 252-254, with appropriate modifications. The specific method was as follows: 1 g of hypocrellin A was dissolved in 1000 mL of 1.5% KOH aqueous solution, the solution was stirred in a lucifugous condition for a reaction for 24 hours and neutralized by using slightly excessive dilute hydrochloric acid, chloroform was used for production extraction, and 0.98 g of hypocrellin B was obtained after separation and purification, with a yield of 98%, and MS (ESI+): 529.3. An absorption spectrum of the extracted hypocrellin A is as shown in FIG. 5(a).

[0131] Preparation of deacetyl hypocrellin (HC): 200 mg of hypocrellin B was dissolved in 100 mL of 1.5% KOH aqueous solution, a reflux reaction was performed for 8 hours in a lucifugous condition, the solution was neutralized by using dilute hydrochloric acid after cooling, dichloromethane was used for production extraction, and 110 mg of deacetyl hypocrellin (HC) was obtained after separation and purification, with a yield of 56%, and MS (ESI+): 487.2. .sup.1H NMR (CDCl.sub.3, S, ppm): 16.0 (s, —OH, 1H), 15.9 (s, —OH, 1H), 6.62 (d, 1H), 6.35 (s, 2H), 4.14, 4.12 (s, —OCH.sub.3, 6H), 4.02 (s, —OCH.sub.3, 3H), 3.1 (d, 2H), 2.25 (s, —OCH.sub.3, 3H).

[0132] Preparation of bromo-hypocrellin B HB—Br and bromo-deacetyl hypocrellin HC—Br: 100 mg of hypocrellin HB or deacetyl hypocrellin HC was dissolved in 100 mL of tetrahydrofuran solvent, and 2 mL of liquid bromine was added dropwise for a reaction at room temperature which was terminated after 6 hours. The reaction solution was treated by adding sodium thiosulfate, extracted by using an organic matter dichloromethane, washed, and dried, and a crude product was separated out by means of thin-layer chromatography, with a developing agent being a mixed solution of acetone:ethyl acetate=1:1 in volume ratio, to respectively obtain 5-bromo-hypocrellin derivatives HB—Br and HC—Br, with yields of 15% and 18%, respectively, and MS (ESI+): 607.5. Structural formulas of the above hypocrellin A (HA), hypocrellin B (HB), deacetyl hypocrellin (HC), and 5-bromo-hypocrellin derivatives HB—Br and HC—Br are as follows:

##STR00023##

Example 2

[0133] Preparation of a di-2-(2-aminoethoxy)ethanol-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—CH.sub.2CH.sub.2—OCH.sub.2CH.sub.2—OH, R.sub.3═—COCH.sub.3, R.sub.4═—H): a synthetic route is as shown in FIG. 2, and specifically includes the following steps: hypocrellin B HB (100 mg, 0.18 mmol) and 2-(2-aminoethoxy)ethanol (2 mmol) were dissolved in 100 mL of anhydrous acetonitrile, after fully mixed, a mixture was heated to 100° C. under the protection of nitrogen and stirred in a lucifugous condition for a reaction for 12 h, a solvent was distilled off after the reaction, a blue black solid was dissolved in 100 mL of dichloromethane, a solution was washed once with 100 mL of a dilute hydrochloric acid and then washed with distilled water three times, an organic layer was dried by using anhydrous magnesium sulfate and filtered, and an organic phase was spin-dried to obtain a crude product. The obtained crude product was separated out by means of thin-layer chromatography, with a developing agent being a mixed solution of acetone:ethyl acetate=1:1 (volume ratio), to obtain four blue black solid products HB-1a-HB-1d, respectively. HB-1a: yield: 4.1%, R.sub.f: 0.35; MS (ESI+): 688.3; maximum absorption wavelength: 630 nm; molar extinction coefficient: 30,000 M.sup.−1cm.sup.−1; and singlet oxygen yield: 35%. HB-1b: yield: 5.2%, R.sub.f: 0.30; MS (ESI+): 688.3; maximum absorption wavelength: 622 nm: molar extinction coefficient: 28,000 M.sup.−1cm.sup.−1; and singlet oxygen yield: 36%. HB-1c: yield: 13.1%, R.sub.f: 0.28; MS (ESI+): 688.3; maximum absorption wavelength: 621 nm; molar extinction coefficient: 30,000 M.sup.−1cm.sup.−1; and singlet oxygen yield: 40%. HB-1d: yield: 3.8%, R.sub.f: 0.32: MS (ESI+): 688.3: maximum absorption wavelength: 622 nm: molar extinction coefficient: 30,000 M.sup.−1cm.sup.−1; and singlet oxygen yield: 35%. Structural formulas of the above amino-substituted products are as follows:

##STR00024##

[0134] An absorption spectrum of the di-2-(2-aminoethoxy)ethanol-substituted hypocrellin B derivative HB-1c is as shown by curve a in FIG. 5(a), and HB-1c has very wide and strong absorption performance in a phototherapy window, has a maximum absorption spectrum wavelength of about 630 nm, which is redshifted by about 180 nm relative to a maximum absorption peak of the parent hypocrellin, and has a molar extinction coefficient of about 30000 M.sup.−1cm.sup.−1, presenting a very high absorptivity in the phototherapy window. The ability of HB-1c to produce reactive oxygen is shown in FIGS. 6(a) and 6(b): the experiments indicate that, it is measured by using singlet oxygen and superoxide radical scavenger that, such the hypocrellin derivative having a peri-position substituted by an amino group can efficiently produce photosensitive reactive species, primarily producing singlet oxygen, as shown in FIG. 6(a), also producing a small amount of superoxide radicals, as shown in FIG. 6(b), and the efficiency of producing singlet oxygen being about 40%. Results of confocal fluorescence imaging experiments shown in FIG. 10 indicate that the phototherapy drug micromolecule HB-1c has good biocompatibility and is able to enter a lysosome of a Hela cell and generate an excellent red-light fluorescence image in the cell.

Example 3

[0135] Preparation of a di-2-(2-aminoethoxy)ethanol-polyethylene glycol (of different chain lengths)-substituted hypocrellin derivative (R.sub.1═R.sub.2═—CH.sub.2CH.sub.2—OCH.sub.2CH.sub.2—OCO-PEGn-OCH.sub.3, R.sub.3═—COCH.sub.3, R.sub.4═—H) (PEG is polyethylene glycol, n is the number of ethylene glycol units, and n=1, 6, 16): hypocrellin B H.sub.8(100 mg, 0.18 mmol) and 2-(2-aminoethoxy)ethanol (2 mmol) were dissolved in 100 mL of anhydrous acetonitrile, after fully mixed, a mixture was heated to 80° C. under the protection of nitrogen and stirred in a lucifugous condition for a reaction for 20 h, a solvent was distilled off after the reaction, a blue black solid was dissolved in 100 mL of dichloromethane, a solution was washed with distilled water three times, an organic layer was dried and filtered, and an organic phase was spin-dried to obtain a crude product. Add DCC (200 mg) to the obtained crude product and dissolved in 50 mL of anhydrous dichloromethane, to react with polyethylene glycol methyl esters (HOOC-PEGn-OCH.sub.3, 2 g) of different chain lengths, respectively, and a reaction solution was stirred in a lucifugous condition at room temperature for a reaction for 8 h. After the reaction, added 100 mL of dichloromethane to the reaction solution, a mixed solution was washed once with 100 mL of a dilute hydrochloric acid aqueous solution and then washed with distilled water three times, an organic layer was dried by using anhydrous magnesium sulfate and filtered, and the crude product was separated out by means of thin-layer chromatography, with a developing agent being a mixed solution of ethyl acetate:ethanol=5:1, to obtain blue black solid products HB-1a-PEGn, HB-1b-PEGn, HB-1c-PEGn, and HB-1d-PEGn (n=1, 6, 16), respectively. HB-1a-PEG1 (n=1): yield: 17.2%, R.sub.f: 0.36; MS (ESI+): 948.4; maximum absorption wavelength: 625 nm: molar extinction coefficient: 31,000 M.sup.−1cm.sup.−11; and singlet oxygen yield: 35%. HB-1b-PEG6 (n=6): yield: 12.5%. R.sub.f: 0.32; MS (ESI+): 1388.6; maximum absorption wavelength: 622 nm: molar extinction coefficient: 30,000 M.sup.−1cm.sup.−1; and singlet oxygen yield: 32%. HB-c-PEG1 (n=1): yield: 21.2%, R.sub.f: 0.31; MS (ESI+): 948.4; maximum absorption wavelength: 626 nm; molar extinction coefficient: 33,000 M.sup.−1cm.sup.−1: and singlet oxygen yield: 38%. HB-1c-PEG6 (n=6): yield: 32.2%, R.sub.f: 0.25: MS (ESI+): 1388.6: maximum absorption wavelength: 628 nm; molar extinction coefficient: 34,500 M.sup.−1cm.sup.−1; and singlet oxygen yield: 34%. HB-1c-PEG16 (n=16): yield: 35.1%, R.sub.f: 0.18; MS (ESI+): 2268.9; maximum absorption wavelength: 624 nm; molar extinction coefficient: 31500 M.sup.−1cm.sup.−1: and singlet oxygen yield: 30%. HB-1d-PEG6 (n=6): yield: 32.2%, R.sub.f: 0.25; MS (ESI+): 1388.6; maximum absorption wavelength: 628 nm; molar extinction coefficient: 34,500 M.sup.−1cm.sup.−1; and singlet oxygen yield: 34%. Structural formulas of the above amino-substituted products are as follows:

##STR00025## ##STR00026##

[0136] Results of confocal fluorescence imaging experiments shown in FIGS. 10(a)-10(c) indicate that the phototherapy drug micromolecule HB-1c-PEG6 has good biocompatibility and is able to enter a lysosome of a Hela cell and generate an excellent red-light fluorescence image in the cell. DCFH-DA is used to detect singlet oxygen in a cell. As shown in FIG. 11, the photosensitizer HC-1c-PEG6 and the fluorescence probe DCFH-DA are co-incubated in the cell, with increase of an irradiation time to 60s, a green fluorescence intensity is gradually increased, indicating that the amount of the singlet oxygen in the cell is increased.

[0137] HB-1c-PEG6 and Hela cells are co-incubated, as shown in FIG. 12(a), a cytotoxicity (dark toxicity) research test indicates that HB-1c-PEG6 has a smaller cytotoxicity, which is similar to that of the hypocrellin B HB and commercial photosensitive drug hematoporphyrin HpD. After the Hela cells are incubated by using the photosensitizer HB-1c-PEG6 with the concentration of 10 μM for a half hour, death of many Hela cells is not observed, indicating that such the photosensitizer is basically non-cytotoxic. A cell phototoxicity experiment as shown in FIG. 12(b) indicates that HB-1c-PEG6 presents very strong lethality to the Hela cells under irradiation of a 635 nm laser. HB-1c-PEG6 with a concentration range of 160 nM can kill more than 90% of the Hela cells, while in the same condition, the hypocrellin B HB or commercial photosensitizer hematoporphyrin derivative HpD can kill only about 30% of the Hela cells, indicating that a photodynamic effect of HB-1c-PEG6 is significantly better than that of the hypocrellin B HB and commercial photosensitizer hematoporphyrin HpD.

Example 4

[0138] Preparation of a di-2-(2-aminoethoxy)ethanol-quaternary ammonium salt-substituted hypocrellin derivative (R.sub.1═R.sub.2═—CH.sub.2CH.sub.2—OCH.sub.2CH.sub.2—OCO—(CH.sub.2).sub.n—N.sup.+(CH.sub.3).sub.3, R.sub.3═—COCH.sub.3, R.sub.4═—H) (n=2, 4, 6): the product HB-1c (20 mg, 0.03 mmol) in example 2 is used as a raw material, added DCC (100 mg), and dissolved in 20 mL of anhydrous dichloromethane, to react with carboxytrimethylamine (HOOC—(CH.sub.2).sub.n—N.sup.4(CH.sub.3).sub.3, 2 g) of different chain lengths, respectively, and a reaction solution was stirred in a lucifugous condition at room temperature for a reaction for 8 h. After the reaction, add 100 mL of dichloromethane to the reaction solution, a mixed solution was washed once with 100 mL of a dilute hydrochloric acid aqueous solution and then washed with distilled water three times, an organic layer was dried by using anhydrous magnesium sulfate and filtered, and a crude product was separated out by means of thin-layer chromatography, with a developing agent being a mixed solution of ethyl acetate:ethanol=5:1, to obtain blue black solid products HB-1a-PEGn, HB-1b-PEGn, HB-1c-PEGn, and HB-d-PEGn (n=2, 4, 6). HB-1c-C2-N.sup.+ (n=2): yield: 27.2%, R.sub.f: 0.36; MS (ESI+): 948.4; maximum absorption wavelength: 625 nm; molar extinction coefficient: 33,000 M.sup.−1cm.sup.−1; and singlet oxygen yield: 35%. HB-1c-C4-N.sup.+ (n=4): yield: 17.2%, R.sub.f: 0.32; MS (ESI+): 1124.1; maximum absorption wavelength: 622 nm; molar extinction coefficient: 31,000 M.sup.1cm.sup.−1; and singlet oxygen yield: 32%. HB-1c-C6-N.sup.+ (n=6): yield: 25.2%, R.sub.f: 0.31; MS (ESI+): 1212.6; maximum absorption wavelength: 626 nm: molar extinction coefficient: 33,000 M.sup.−1cm.sup.−1; and singlet oxygen yield: 38%. Structural formulas of the above amino-substituted products are as follows:

##STR00027##

[0139] The above prepared compound HB-1c-C2-N.sup.+ contains two quaternary ammonium salts and two ethylene glycol units, making the photosensitizer molecules have very high water solubility in a physiological condition; and each milliliter of normal saline can dissolve more than 20 mg of photosensitizer molecules, presenting excellent water solubility. Therefore, the photosensitive drug can be well transported in blood vessels during intravenous injection, without causing a vascular blockage.

Example 5

[0140] Preparation of a di-2-(2-aminoethoxy)ethanol-substituted deacetyl hypocrellin derivative (R.sub.1═R.sub.2═—CH.sub.2CH—OCH.sub.2CH.sub.2—OH, R.sub.1═R.sub.4═—H): a synthetic method is as shown in FIG. 3, and specifically includes the following steps: deacetyl hypocrellin HC (100 mg, 0.20 mmol) and 2-(2-aminoethoxy)ethanol (2 mmol) were dissolved in 100 mL of anhydrous acetonitrile, after fully mixed, a mixture was heated to 100° C. under the protection of nitrogen and stirred in a lucifugous condition for a reaction for 15 h, after the reaction, a solvent was removed by means of rotary evaporation, a blue black solid was dissolved in 100 mL of dichloromethane, a solution was washed once with 100 mL of a dilute hydrochloric acid aqueous solution and then washed with distilled water three times, an organic layer was dried and filtered, and an organic phase was spin-dried to obtain a crude product. The obtained crude product was separated out by means of thin-layer chromatography, with a developing agent being a mixed solution of acetone:ethyl acetate=2:1 in volume ratio, to obtain four blue black solid products HC-1a-HC-1d, respectively. HC-1a: yield: 5.6%, R.sub.f: 0.30: MS (ESI+): 646.8; maximum absorption wavelength: 628 nm; molar extinction coefficient: 28,000 M.sup.−1cm.sup.−1; and singlet oxygen yield: 36%. HC-1b: yield: 4.2%, R.sub.f: 0.28; MS (ESI+): 646.8; maximum absorption wavelength: 624 nm; molar extinction coefficient: 27000 M.sup.−1cm.sup.−1; and singlet oxygen yield: 33%. HC-1c: yield: 13.1%, R.sub.f: 0.26; MS (ESI+): 646.8; maximum absorption wavelength: 6351 nm; molar extinction coefficient: 30,000 M.sup.−1cm.sup.−1; and singlet oxygen yield: 38%. HC-1d: yield: 4.8%, R.sub.f: 0.24: MS (ESI+): 646.8; maximum absorption wavelength: 626 nm; molar extinction coefficient: 28,500 M.sup.−1cm.sup.−1; and singlet oxygen yield: 32%. Structural formulas of the above amino-substituted products are as follows:

##STR00028##

[0141] An absorption spectrum of the di-2-(2-aminoethoxy)ethanol-substituted deacetyl hypocrellin derivative Hc-1c is as shown by curve b in FIG. 5(a), and HB-1c has very wide and strong absorption performance in a phototherapy window, has a maximum absorption spectrum wavelength of about 640 nm, which is redshifted by about 190 nm relative to a maximum absorption peak of the parent hypocrellin, and has a molar extinction coefficient of about 30000 M.sup.−1cm.sup.−1, presenting a very high absorptivity in the phototherapy window. In addition, as shown in FIG. 8, HC-1c has an excellent pH stability, and there is no obvious change in an absorption spectrum thereof in the pH range of 6.2-8.0.

Example 6

[0142] Preparation of a di-2-(2-aminoethoxy)ethanol-polyethylene glycol (of different chain lengths)-substituted deacetyl hypocrellin derivative (R.sub.1═R.sub.2═—CH.sub.2CH.sub.2—OCO-PEGn-OCH.sub.3, R.sub.3═—H) (PEG is polyethylene glycol, n is the number of ethylene glycol units, and n=1, 6, 12): deacetyl hypocrellin HC (100 mg, 0.20 mmol) and 2-(2-aminoethoxy)ethanol (2 mmol) were dissolved in 100 mL of anhydrous acetonitrile, after fully mixed, a mixture was heated to 80° C. under the protection of nitrogen and stirred in a lucifugous condition for a reaction for 20 h, a solvent was distilled off after the reaction, a blue black solid was dissolved in 100 mL of dichloromethane, a solution was washed with distilled water three times, an organic layer was dried and filtered, and an organic phase was spin-dried to obtain a crude product. Add DCC (200 mg) to the obtained crude product and dissolved in 50 mL of anhydrous dichloromethane, to react with polyethylene glycol methyl esters (HOOC-PEGn-OCH.sub.3, 2 g) of different chain lengths, respectively, and a reaction solution was stirred in a lucifugous condition at room temperature for a reaction for 8 h. After the reaction, the reaction solution was added to 100 mL of dichloromethane, a mixed solution was washed once with 100 mL of a dilute hydrochloric acid aqueous solution and then washed with distilled water three times, an organic layer was dried by using anhydrous magnesium sulfate and filtered, and the crude product was separated out by means of thin-layer chromatography, with a developing agent being a mixed solution of ethyl acetate:ethanol=5:1, to obtain blue black solid products HC-1a-PEGn, HC-1b-PEGn, HC-1c-PEGn, and HC-1d-PEGn (n=1, 6, 12), respectively. HC-1a-PEG1 (n=1): yield: 13.2%, R.sub.f: 0.35; MS (ESI+): 906.4; maximum absorption wavelength: 622 nm; molar extinction coefficient: 30,500 M.sup.−1cm.sup.−1; and singlet oxygen yield: 32%. HC-1b-PEG6 (n=6): yield: 10.5%. R.sub.f: 0.31; MS (ESI+): 1346.6; maximum absorption wavelength: 624 nm; molar extinction coefficient: 30,000 M.sup.−1cm.sup.−1; and singlet oxygen yield: 30%. HC-1c-PEG1 (n=1): yield: 16.2%. R.sub.f: 0.30; MS (ESI+): 906.4; maximum absorption wavelength: 625 nm; molar extinction coefficient: 32,500 M.sup.−1cm.sup.−1; and singlet oxygen yield: 34%. HC-1c-PEG6 (n=6): yield: 18.2%, R.sub.f: 0.26; MS (ESI+): 1346.6; maximum absorption wavelength: 628 nm; molar extinction coefficient: 33,500 M.sup.−1cm.sup.−1; and singlet oxygen yield: 34%. HC-1c-PEG12 (n=12): yield: 17.1%, R.sub.f: 0.18; MS (ESI+): 1874.9; maximum absorption wavelength: 626 nm; molar extinction coefficient: 32,500 M.sup.−1cm.sup.−1; and singlet oxygen yield: 35%. HC-1d-PEG6 (n=6): yield: 13.2%, R.sub.f: 0.22: MS (ESI+): 1346.6; maximum absorption wavelength: 622 nm; molar extinction coefficient: 34,500 M.sup.−1cm.sup.−1; and singlet oxygen yield: 34%. Structural formulas of the above amino-substituted products are as follows:

##STR00029## ##STR00030##

[0143] The above prepared compound HC-1c-PEG6 (n=6) contains two 6-PEG long-chains, making the photosensitizer molecules have very high water solubility in a physiological condition; and each milliliter of normal saline can dissolve more than 20 mg of photosensitizer molecules, presenting excellent water solubility. Therefore, the photosensitive drug can be well transported in blood vessels during intravenous injection, without causing a vascular blockage. In addition, as shown in FIG. 8, HC-1c-PEG6 also has an excellent pH stability, and there is no obvious change in an absorption spectrum thereof in the pH range of 6.2-8.0, for the reason that two phenolic hydroxyl groups of the hypocrellin are not prone to deprotonation in this acid-base condition. However, the commercial hematoporphyrin HpD contains two carboxyl groups, which can be deprotonated in the pH range of 6.2-8.0, resulting in an obvious change in the absorption spectrum, thus presenting an instability of the HpD photosensitizer.

[0144] FIGS. 13(a) and 13(b) show experimental results of dark cytotoxicity and phototoxicity of HB-1c-PEG6. It can be seen that HC-c-PEG6 has almost no cytotoxicity when not exposed to light. After exposure to 635 nm light, HC-1c-PEG6 with a concentration range of 140 nM can kill more than 90% of the Hela cells, while in the same condition, the deacetyl hypocrellin HC can kill 40% of the Hela cells, and the commercial photosensitizer hematoporphyrin derivative HpD can kill only about 10% of the Hela cells, indicating that a photodynamic effect of HB-c-PEG6 is significantly better than that of the deacetyl hypocrellin HC and commercial photosensitizer hematoporphyrin HpD.

Example 7

[0145] Preparation of an aminoethanol-substituted hypocrellin derivative (R.sub.1═R.sub.2═—CH.sub.2CH.sub.2—OH, R.sub.3═—COCH.sub.3, R.sub.4═—H): a substituted amino raw material is NH.sub.2—CH.sub.2CH.sub.2—OH, a synthetic method is similar to the preparation of the di-2-(2-aminoethoxy)ethanol-substituted hypocrellin B derivative in example 2, and four blue black solid products HB-2a-HB-2d are obtained, respectively. HB-2a: yield: 4.2%, R.sub.f: 0.36; MS (ESI+): 600.1; maximum absorption wavelength: 625 nm; molar extinction coefficient: 30,500 M.sup.−1cm.sup.−1; and singlet oxygen yield: 28%. HB-2b: yield: 4.7%. R.sub.f: 0.32; MS (ESI+): 600.1; maximum absorption wavelength: 627 nm; molar extinction coefficient: 31.500 M.sup.−1cm.sup.−1; and singlet oxygen yield: 25%. HB-2c: yield: 12.7%, R.sub.f: 0.39; MS (ESI+): 600.1; maximum absorption wavelength: 628 nm; molar extinction coefficient: 31,000 M.sup.−1cm.sup.−1; and singlet oxygen yield: 30%. HB-2d: yield: 4.6%, R.sub.f: 0.29; MS (ESI+): 600.1; maximum absorption wavelength: 629 nm; molar extinction coefficient: 30,000 M.sup.−1cm.sup.−1; and singlet oxygen yield: 26%. Structural formulas of the above amino-substituted products are as follows:

##STR00031##

Example 8

[0146] Preparation of an aminoethanol-polyethylene glycol (of different chain lengths)-substituted hypocrellin derivative (R.sub.1═R.sub.2═—CH.sub.2CH.sub.2—OCO-PEGn-OCH.sub.3, R.sub.3═—COCH.sub.3, R.sub.4═—H) (PEG is polyethylene glycol, n is the number of ethylene glycol units, and n=1, 6, 12): hypocrellin B HB (100 mg, 0.18 mmol) and 2-(2-aminoethoxy)ethanol (2 mmol) were dissolved in 100 mL of anhydrous acetonitrile, after fully mixed, a mixture was heated to 100° C. under the protection of nitrogen and stirred in a lucifugous condition for a reaction for 12 h, a solvent was distilled off after the reaction, a blue black solid was dissolved in 100 mL of dichloromethane, a solution was washed with distilled water three times, an organic layer was dried and filtered, and an organic phase was spin-dried to obtain a crude product. The obtained crude product was added to DCC (200 mg) and dissolved in 50 mL of anhydrous dichloromethane, to react with polyethylene glycol methyl esters (HOOC-PEGn-OCH.sub.3, 2 g) of different chain lengths, respectively, and a reaction solution was stirred in a lucifugous condition at room temperature for a reaction for 8 h. After the reaction, the reaction solution was added to 100 mL of dichloromethane, a mixed solution was washed once with 100 mL of a dilute hydrochloric acid aqueous solution and then washed with distilled water three times, an organic layer was dried by using anhydrous magnesium sulfate and filtered, and the crude product was separated out by means of thin-layer chromatography, with a developing agent being a mixed solution of ethyl acetate:ethanol=5:1, to obtain blue black solid products HB-2a-PEGn, HB-2b-PEGn, HB-2c-PEGn, and HB-2d-PEGn (n=1, 6, 12), respectively. HB-2a-PEG1 (n=1): yield: 12.2%, R.sub.f: 0.34; MS (ESI+): 860.3; maximum absorption wavelength: 620 nm: molar extinction coefficient: 30,500 M.sup.−1cm.sup.−1; and singlet oxygen yield: 30%. HB-2b-PEG6 (n=6): yield: 8.5%, R.sub.f: 0.32; MS (ESI+): 1300.6; maximum absorption wavelength: 622 nm; molar extinction coefficient: 31,000 M.sup.−1cm.sup.−1; and singlet oxygen yield: 32%. HB-2c-PEG6 (n=6): yield: 18.4%. R.sub.f: 0.26; MS (ESI+): 1300.6; maximum absorption wavelength: 628 nm; molar extinction coefficient: 33,000 M.sup.−1cm.sup.−1; and singlet oxygen yield: 35%. HB-2c-PEG12 (n=12): yield: 17.1%, R.sub.f: 0.18; MS (ESI+): 1828.9; maximum absorption wavelength: 626 nm: molar extinction coefficient: 32,500 M.sup.−1cm.sup.−1; and singlet oxygen yield: 36%. HB-2c-PEG6 (n=6): yield: 12.2%, R.sub.f: 0.20: MS (ESI+): 1300.6; maximum absorption wavelength: 624 nm; molar extinction coefficient: 31500 M.sup.−1cm.sup.−1; and singlet oxygen yield: 30%. HB-1d-PEG6 (n=6): yield: 12.2%, R.sub.f: 0.20: MS (ESI+): 1300.6; maximum absorption wavelength: 622 nm; molar extinction coefficient: 32,500 M.sup.−1cm.sup.−1; and singlet oxygen yield: 30%. Structural formulas of the above amino-substituted products are as follows:

##STR00032## ##STR00033##

Example 9

[0147] Preparation of an aminoethanol-substituted deacetyl hypocrellin derivative (R.sub.1═R.sub.2═—CH.sub.2CH—OH, R.sub.3═R.sub.4═—H): a substituted amino raw material is NH.sub.2—CHCH.sub.2—OH, a synthetic method is similar to the preparation of the di-2-(2-aminoethoxy)ethanol-substituted deacetyl hypocrellin B derivative in example 5, and four blue black solid products HC-2a-HC-2d are obtained, respectively. HC-2a: yield: 5.8%, R.sub.f: 0.28; MS (ESI+): 558.8; maximum absorption wavelength: 627 nm; molar extinction coefficient: 28.500 M.sup.−1cm.sup.−1; and singlet oxygen yield: 32%. HC-2b: yield: 3.8%. R.sub.f: 0.26: MS (ESI+): 558.8; maximum absorption wavelength: 624 nm; molar extinction coefficient: 27.500 M.sup.−1cm.sup.−1; and singlet oxygen yield: 33%. HC-2c: yield: 12.1%, R.sub.f: 0.24; MS (ESI+): 558.8; maximum absorption wavelength: 636 nm; molar extinction coefficient: 31,000 M.sup.−1cm.sup.−1; and singlet oxygen yield: 40%. HC-2d: yield: 5.2%, R.sub.f: 0.20; MS (ESI+): 558.8 maximum absorption wavelength: 625 nm: molar extinction coefficient: 28,500 M.sup.−1cm.sup.−1; and singlet oxygen yield: 34%. Structural formulas of the above amino-substituted products are as follows:

##STR00034##

[0148] As shown in FIG. 9, after being irradiated with a 635 nm laser for 30 min at a light intensity of 20 mW/cm.sup.2, an absorption spectrum of the above prepared compound HC-2c is not obviously decreased, and an absorption intensity at the maximum wavelength is decreased by less than 10%, indicating that HC-2c has a good light stability, and the photosensitizer does not lose efficacy under continuous laser irradiation.

Example 10

[0149] Preparation of an aminoethanol-polyethylene glycol (of different chain lengths)-substituted deacetyl hypocrellin derivative (R.sub.1═R.sub.2═—CH.sub.2CH.sub.2—OCO-PEGn-OCH.sub.3, R.sub.3═R.sub.4═—H) (PEG is polyethylene glycol, n is the number of ethylene glycol units, and n=1, 6, 12): deacetyl hypocrellin HC (100 mg, 0.201 mmol) and 2-(2-aminoethoxy)ethanol (2 mmol) were dissolved in 100 mL of anhydrous acetonitrile, after fully mixed, a mixture was heated to 100° C. under the protection of nitrogen and stirred in a lucifugous condition for a reaction for 12 h, a solvent was distilled off after the reaction, a blue black solid was dissolved in 100 mL of dichloromethane, a solution was washed with distilled water three times, an organic layer was dried and filtered, and an organic phase was spin-dried to obtain a crude product. The obtained crude product was added to DCC (200 mg) and dissolved in 50 mL of anhydrous dichloromethane, to react with polyethylene glycol methyl esters (HOOC-PEGn-OCH.sub.3, 2 g) of different chain lengths, respectively, and a reaction solution was stirred in a lucifugous condition at room temperature for a reaction for 8 h. After the reaction, the reaction solution was added to 100 mL of dichloromethane, a mixed solution was washed once with 100 mL of dilute hydrochloric acid aqueous solution and then washed with distilled water three times, an organic layer was dried by using anhydrous magnesium sulfate and filtered, and the crude product was separated out by means of thin-layer chromatography, with a developing agent being a mixed solution of ethyl acetate:ethanol=5:1, to obtain blue black solid products HC-2a-PEGn, HC-2b-PEGn, HC-2c-PEGn, and HC-2d-PEGn (n=1, 6, 12), respectively. HC-2a-PEG1 (n=1): yield: 11.2%, R.sub.f: 0.34; MS (ESI+): 818.4; maximum absorption wavelength: 622 nm; molar extinction coefficient: 30,000 M.sup.−1cm.sup.−1; and singlet oxygen yield: 30%. HC-2b-PEG6 (n=6): yield: 10.8%. R.sub.f: 0.31; MS (ESI+): 1258.6; maximum absorption wavelength: 625 nm molar extinction coefficient: 30,500 M.sup.−1cm.sup.−1; and singlet oxygen yield: 31%. HC-2c-PEG6 (n=6): yield: 18.6%. R.sub.f: 0.25; MS (ESI+): 1258.6; maximum absorption wavelength: 628 nm; molar extinction coefficient: 33,000 M.sup.−1cm.sup.−1; and singlet oxygen yield: 35%. HC-2c-PEG12 (n=12): yield: 18.1%, R.sub.f: 0.20; MS (ESI+): 1786.9; maximum absorption wavelength: 630 nm: molar extinction coefficient: 32,500 M.sup.−1cm.sup.−1; and singlet oxygen yield: 34%. HC-2d-PEG6 (n=6): yield: 12.2%. R.sub.f: 0.24; MS (ESI+): 1258.6: maximum absorption wavelength: 624 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; and singlet oxygen yield: 32%. Structural formulas of the above amino-substituted products are as follows:

##STR00035## ##STR00036##

[0150] As shown in FIG. 9, after being irradiated with a 635 nm laser for 30 min at a light intensity of 20 mW/cm.sup.2, an absorption spectrum of the above prepared compound HC-2d-PEG6 is not obviously decreased, and an absorption intensity at the maximum wavelength is decreased by less than 10%, indicating that HC-2d-PEG6 has a good light stability, and the photosensitizer does not lose efficacy under continuous laser irradiation. However, in the same condition, after being irradiated with a 635 nm laser for 30 min at a light intensity of 20 mW/cm.sup.2, maximum absorption performance of the commercial porphin photosensitizer Ce6 is decreased by 30%, and an absorption spectrum of the commercial hematoporphyrin photosensitizer HpD is decreased more, reaching about 50%. Therefore, in the same condition, HC-2d-PEG6 has a better photostability than the commercial photosensitizers.

Example 11

[0151] Preparation of a diaminoethyl-polyethylene glycol monomethyl ether (of different chain lengths)-substituted hypocrellin derivative (R.sub.1═R.sub.2═—CH.sub.2CH.sub.2—PEGn-OH, R.sub.3═—COCH.sub.3, R.sub.4═—H) (PEG is polyethylene glycol, n is the number of ethylene glycol units, and n=4, 8, 12): a substituted amino group is NH.sub.2—CH.sub.2CH.sub.2—PEGn-OH, a synthetic method is similar to the preparation of the di-2-(2-aminoethoxy)ethanol-substituted hypocrellin B derivative in example 2, and four blue black solid products HB-3a-PEGn, HB-3b-PEGn, HB-3c-PEGn, and HB-3d-PEGn (n=4, 8, 12) are obtained, respectively. HB-3a-PEG4 (n=4): yield: 6.2%, R.sub.f: 0.24; MS (ESI+): 980.3; maximum absorption wavelength: 622 nm; molar extinction coefficient: 29,500 M.sup.−1cm.sup.−1; and singlet oxygen yield: 28%. HB-3b-PEG6 (n=8): yield: 7.5%. R.sub.f: 0.22; MS (ESI+): 1332.6; maximum absorption wavelength: 624 nm; molar extinction coefficient: 29,000 M.sup.−1cm.sup.−1; and singlet oxygen yield: 30%. HB-3c-PEG6 (n=8): yield: 10.4%. R.sub.f: 0.16; MS (ESI+): 1332.6; maximum absorption wavelength: 628 nm; molar extinction coefficient: 30,000 M.sup.−1cm.sup.−1; and singlet oxygen yield: 33%. HB-3c-PEG12 (n=12): yield: 10.1%, R.sub.f: 0.12; MS (ESI+): 1684.9; maximum absorption wavelength: 626 nm: molar extinction coefficient: 31,000 M.sup.−1cm.sup.−1; and singlet oxygen yield: 35%. HB-3d-PEG6 (n=8): yield: 5.2%, R.sub.f: 0.10; MS (ESI+): 1332.6; maximum absorption wavelength: 621 nm; molar extinction coefficient: 30,500 M.sup.−1cm.sup.−1; and singlet oxygen yield: 30%. Structural formulas of the above amino-substituted products are as follows:

##STR00037##

Example 12

[0152] Preparation of a diaminoethyl-polyethylene glycol monomethyl ether (of different chain lengths)-substituted deacetyl hypocrellin derivative (R.sub.1═R.sub.2═—CH.sub.2CH.sub.2—PEGn-OH, R.sub.3═R.sub.4═—H) (PEG is polyethylene glycol, n is the number of ethylene glycol units, and n=4, 8, 12): a substituted amino group is NH.sub.2—CH.sub.2CH.sub.2—PEGn-OH, a synthetic method is similar to the preparation of the di-2-(2-aminoethoxy)ethanol-substituted hypocrellin B derivative in example 2, and four blue black solid products HC-3a-PEGn, HC-3b-PEGn, HC-3c-PEGn, and HC-3d-PEGn (n=4, 8, 12) are obtained, respectively. HC-3a-PEG4 (n=4): yield: 5.4%, R.sub.f: 0.25; MS (ESI+): 938.3; maximum absorption wavelength: 622 nm; molar extinction coefficient: 29,000 M.sup.−1cm.sup.−1; and singlet oxygen yield: 28%. HC-3b-PEG6 (n=8): yield: 7.1%. R.sub.f: 0.22; MS (ESI+): 1290.6; maximum absorption wavelength: 623 nm; molar extinction coefficient: 29,500 M.sup.−1cm.sup.−1; and singlet oxygen yield: 32%. HC-3c-PEG6 (n=8): yield: 11.2%, R.sub.f: 0.18; MS (ESI+): 1290.6; maximum absorption wavelength: 630 nm; molar extinction coefficient: 30,500 M.sup.−1cm.sup.−1; and singlet oxygen yield: 33%. HC-3d-PEG12 (n=12): yield: 8.1%, R.sub.f: 0.15; MS (ESI+): 1642.9; maximum absorption wavelength: 628 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; and singlet oxygen yield: 36%. HC-3d-PEG6 (n=8): yield: 4.8%, R.sub.f: 0.12; MS (ESI+): 1290.6; maximum absorption wavelength: 621 nm; molar extinction coefficient: 30,000 M.sup.−1cm.sup.−1; and singlet oxygen yield: 32%. Structural formulas of the above amino-substituted products are as follows:

##STR00038##

Example 13

[0153] Preparation of a diaminoethyl-polyethylene glycol monomethyl ether (of different chain lengths)-substituted hypocrellin derivative (R.sub.1═R.sub.2═—CH.sub.2CH.sub.2—PEGn-OCH.sub.3, R.sub.3═R.sub.4═—H) (PEG is polyethylene glycol, n is the number of ethylene glycol units, and n=4, 8, 16): a substituted amino group is NH.sub.2—CH.sub.2CH.sub.2—PEGn-OCH.sub.3, a synthetic method is similar to the preparation of the di-2-(2-aminoethoxy)ethanol-substituted hypocrellin B derivative in example 2, and four blue black solid products HC-4a-PEGn, HC-4b-PEGn, HC-4c-PEGn, and HC-4d-PEGn (n=4, 8, 16) are obtained, respectively. HC-4a-PEGn (n=4): yield: 5.4%. R.sub.f: 0.25: MS (ESI+): 924.3; maximum absorption wavelength: 622 nm; molar extinction coefficient: 29,000 M.sup.−1cm.sup.−1; and singlet oxygen yield: 28%. HC-4b-PEG6 (n=8): yield: 7.1%, R.sub.f: 0.22; MS (ESI+): 1276.6; maximum absorption wavelength: 623 nm: molar extinction coefficient: 29,500 M.sup.−1cm.sup.−1; and singlet oxygen yield: 32%. HC-4c-PEG6 (n=8): yield: 11.2%, R.sub.f: 0.18; MS (ESI+): 1276.6; maximum absorption wavelength: 630 nm; molar extinction coefficient: 30,500 M.sup.−1cm.sup.−1; and singlet oxygen yield: 33%. HC-4c-PEG16 (n=16): yield: 8.1%, R.sub.f: 0.15; MS (ESI+): 1978.9; maximum absorption wavelength: 628 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; and singlet oxygen yield: 36%. HC-4d-PEG6 (n=8): yield: 4.8%, R.sub.f: 0.12; MS (ESI+): 1276.6; maximum absorption wavelength: 621 nm; molar extinction coefficient: 30,000 M.sup.−1cm.sup.−1; and singlet oxygen yield: 32%. Structural formulas of the above amino-substituted products are as follows:

##STR00039##

Example 14

[0154] Preparation of an ethylene diamine-polyethylene glycol (of different chain lengths)-substituted hypocrellin derivative (R.sub.1═R.sub.2═—CH.sub.2CH.sub.2—NH—CH.sub.2CH.sub.2—PEGn-OH, R.sub.3═—COCH.sub.3, R.sub.4═—H) (PEG is polyethylene glycol, n is the number of ethylene glycol units, and n=1, 4): a substituted amino raw material is NH.sub.2—CHCH—NH—CH.sub.2CH.sub.2—PEGn-OH, a synthetic method is similar to the preparation of the di-2-(2-aminoethoxy)ethanol-substituted hypocrellin B derivative in example 2, and four blue black solid products HB-5a-PEGn, HB-5b-PEGn, HB-5c-PEGn, and HB-5d-PEGn (n=1, 4) are obtained, respectively. HB-5a-PEG1 (n=1): yield: 6.4%, R.sub.f: 0.24; MS (ESI+): 774.3; maximum absorption wavelength: 622 nm: molar extinction coefficient: 29,500 M.sup.−1cm.sup.−1; and singlet oxygen yield: 28%. HB-5b-PEG4 (n=4): yield: 8.1%, R.sub.f: 0.28; MS (ESI+): 1038.6; maximum absorption wavelength: 625 nm; molar extinction coefficient: 30,500 M.sup.−1cm.sup.−1; and singlet oxygen yield: 31%. HB-5c-PEG4 (n=4): yield: 10.2%. R.sub.f: 0.30: MS (ESI+): 1038.6; maximum absorption wavelength: 628 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; and singlet oxygen yield: 35%. HB-5d-PEG1 (n=1): yield: 4.6%, R.sub.f 0.15; MS (ESI+): 774.6; maximum absorption wavelength: 625 nm: molar extinction coefficient: 30,500M.sup.−1cm.sup.−1; and singlet oxygen yield: 32%. Structural formulas of the above amino-substituted products are as follows:

##STR00040##

Example 15

[0155] Preparation of an ethylene diamine-polyethylene glycol (of different chain lengths)-substituted bromo-hypocrellin derivative (R.sub.1═R.sub.2═—CHCH.sub.2—NH—CH.sub.2CH.sub.2—PEGn-OH, R.sub.3═—H, R.sub.4═—Br) (PEG is polyethylene glycol, n is the number of ethylene glycol units, and n=1, 4): a substituted amino raw material is NH.sub.2—CH.sub.2CH.sub.2—NH—CH.sub.2CH.sub.2—PEGn-OH, a synthetic method is similar to the preparation of the di-2-(2-aminoethoxy)ethanol-substituted hypocrellin B derivative in example 2, and four blue black solid products HB-5a-Br-PEGn, HB-5b-Br-PEGn, HB-5c-Br-PEGn, and HB-5d-Br-PEGn (n=1, 4) are obtained, respectively. HB-5a-Br-PEG1 (n=1): yield: 7.4%, R.sub.f: 0.22; MS (ESI+): 854.3; maximum absorption wavelength: 622 nm; molar extinction coefficient: 30,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 28%. HB-5b-Br-PEG4 (n=4): yield: 8.5%, R.sub.f: 0.25; MS (ESI+): 1118.6; maximum absorption wavelength: 625 nm; molar extinction coefficient: 30,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 30%. HB-5c-Br-PEG4 (n=4): yield: 10.5%, R.sub.f: 0.33: MS (ESI+): 1118.6; maximum absorption wavelength: 630 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 36%. HB-5d-Br-PEG1 (n=1): yield: 6.6%, R.sub.f: 0.18; MS (ESI+): 854.6; maximum absorption wavelength: 624 nm; molar extinction coefficient: 29,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. Structural formulas of the above amino-substituted products are as follows:

##STR00041##

Example 16

[0156] Preparation of a diamino-thiopolyethylene glycol-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—CHCH—SCH.sub.2CH.sub.2—PEGn-OH, R.sub.3═—COCH.sub.3, R.sub.4═—H) (PEG is polyethylene glycol, n is the number of ethylene glycol units, and n=1, 4): a substituted amino raw material is NH.sub.2—SCH.sub.2CH.sub.2—PEGn-OH, a synthetic method is similar to the preparation of the di-2-(2-aminoethoxy)ethanol-substituted hypocrellin B derivative in example 2, and four blue black solid products HB-6a-PEGn, HB-6b-PEGn, HB-6c-PEGn, and HB-6d-PEGn (n=1, 4) are obtained, respectively. HB-6a-PEG1 (n=1): yield: 7.4%. R.sub.f: 0.26; MS (ESI+): 791.3; maximum absorption wavelength: 622 nm; molar extinction coefficient: 30,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 28%. HB-6b-PEG4 (n=4): yield: 9.1%, R.sub.f: 0.28; MS (ESI+): 1055.6; maximum absorption wavelength: 624 nm: molar extinction coefficient: 30,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HB-6c-PEG4 (n=4): yield: 12.2%, R.sub.f: 0.30; MS (ESI+): 1055.6; maximum absorption wavelength: 630 nm; molar extinction coefficient: 32,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 36%. HB-6d-PEG1 (n=1): yield: 5.6%, R.sub.f: 0.12: MS (ESI+): 791.6; maximum absorption wavelength: 625 nm; molar extinction coefficient: 30,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. Structural formulas of the above amino-substituted products are as follows:

##STR00042##

Example 17

[0157] Preparation of a diaminoacetic acid-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—CH.sub.2COOH, R.sub.3═—COCH.sub.3, R.sub.4═—H): hypocrellin B HB (100 mg, 0.18 mmol), aminoacetic acid (10 mmol), and NaOH (2 g) were dissolved in 100 mL of a mixed solution of DMF and water (at a volume ratio of 1:1), and after fully mixed, a mixed solution was heated to 120° C. under the protection of nitrogen and stirred in a lucifugous condition for a reaction for 10 h. After the reaction, dilute hydrochloric acid was added to adjust the pH to weak acidity, and filtration was performed to collect a precipitate. A blue black solid was dissolved in 200 mL of dichloromethane, a solution was washed once with 100 mL of a dilute hydrochloric acid aqueous solution and then washed twice with distilled water, an organic layer was dried by using anhydrous magnesium sulfate and filtered, and an organic phase was spin-dried to obtain a crude product. The obtained crude product was separated out by means of thin-layer chromatography, with a developing agent being a mixed solution of ethyl acetate, diethylamine, and ethanol (at a volume ratio of 20:1:2), to obtain four blue black solid products HB-7a-HB-7d, respectively. HB-7a: yield: 7.4%, R.sub.f: 0.32; MS (ESI+): 628.9; maximum absorption wavelength: 620 nm; molar extinction coefficient: 26,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 28%. HB-7b: yield: 6.2%, R.sub.f: 0.35; MS (ESI+): 628.9; maximum absorption wavelength: 622 nm; molar extinction coefficient: 28,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 25%. HB-7c: yield: 15.8%, R.sub.f: 0.24; MS (ESI+): 628.9; maximum absorption wavelength: 618 nm; molar extinction coefficient: 27,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 27%. HB-7d: yield: 4.8%, R.sub.f: 0.28; MS (ESI+): 628.9; maximum absorption wavelength: 623 nm; molar extinction coefficient: 25,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 25%. Structural formulas of the above amino-substituted products are as follows:

##STR00043##

Example 18

[0158] Preparation of a diaminoacetic acid-substituted deacetyl hypocrellin derivative (R.sub.1═R.sub.2═—CH.sub.2COOH, R.sub.3═R.sub.4═—H): deacetyl hypocrellin HC was used as a raw material, a synthetic method is similar to the preparation of the diaminoacetic acid-substituted hypocrellin B derivative in example 17, and four blue black solid products are obtained, respectively. HC-7a: yield: 5.8%, R.sub.f: 0.30; MS (ESI+): 586.9; maximum absorption wavelength: 620 nm; molar extinction coefficient: 27,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 28%. HC-7b: yield: 6.6%. R.sub.f: 0.33 MS (ESI+): 586.9; maximum absorption wavelength: 622 nm; molar extinction coefficient: 28,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 25%. HC-7c: yield: 12.8%, R.sub.f: 0.22; MS (ESI+): 586.9; maximum absorption wavelength: 630 nm; molar extinction coefficient: 29,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 27%. HC-7d: yield: 3.8%, R.sub.f: 0.26; MS (ESI+): 586.9; maximum absorption wavelength: 626 nm; molar extinction coefficient: 26.000 M.sup.−1cm.sup.−1; singlet oxygen yield: 25%. Structural formulas of the above amino-substituted products are as follows:

##STR00044##

Example 19

[0159] Preparation of a diaminobutyric acid-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—CH.sub.2CH.sub.2CH.sub.2HOOH, R═—COCH.sub.3, R.sub.4═—H): a synthetic route is as shown in FIG. 4, a substituted amino raw material is an aminobutyric acid, a synthetic method is similar to the preparation of the diaminoacetic acid-substituted hypocrellin B derivative in example 17, and four blue black solid products HB-8a-HB-8d are obtained, respectively. HB-8a: yield: 4.4%, R.sub.f: 0.36; MS (ESI+): 684.6; maximum absorption wavelength: 622 nm; molar extinction coefficient: 27,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 25%. HB-8b: yield: 6.2%, R.sub.f: 0.32: MS (ESI+): 684.6; maximum absorption wavelength: 618 nm: molar extinction coefficient: 28,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 26%. HB-8c: yield: 5.4%, R.sub.f: 0.22; MS (ESI+): 684.6; maximum absorption wavelength: 620 nm; molar extinction coefficient: 29,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 30%. HB-8d: yield: 4.6%, R.sub.f: 0.20; MS (ESI+): 684.6; maximum absorption wavelength: 624 nm molar extinction coefficient: 29,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 26%.

[0160] As shown in FIGS. 5(a) and 5(b), the hypocrellin derivative having a peri-position substituted by an amino group (HB-2a) of the present invention has very wide and strong absorption performance in the phototherapy window, has a maximum absorption spectrum wavelength reaching about 650 nm, which is redshifted by about 200 nm relative to a maximum absorption peak (450 nm) of the parent hypocrellin, and has a molar extinction coefficient of about 30000 M.sup.−1cm.sup.−1, presenting an extremely strong red-light absorption capability.

[0161] As shown in FIGS. 6(a) and 6(b): the experiments indicate that, it is measured by using singlet oxygen and superoxide radical scavengers that, the hypocrellin derivative having a peri-position substituted by an amino group can efficiently produce photosensitive reactive species, primarily producing singlet oxygen, and also producing a small amount of superoxide radicals. The photosensitizer molecule HB-8a contains two water-soluble carboxyl groups, making the photosensitizer molecules have very high water solubility in a physiological condition; and experiments indicate that each milliliter of normal saline can dissolve more than 5 mg of the photosensitizer molecules, presenting excellent water solubility. Therefore, the photosensitive drug HB-8a can be well transported in blood vessels during intravenous injection, without causing a vascular blockage. Structural formulas of the above amino-substituted products are as follows:

##STR00045##

[0162] The above prepared compound HB-8c contains two carboxylic acid groups, making the photosensitizer molecules have good water solubility in a physiological condition; and each milliliter of normal saline can dissolve more than 10 mg of photosensitizer molecules. Therefore, the photosensitive drug can be well transported in blood vessels during intravenous injection, without causing avascular blockage.

Example 20

[0163] Preparation of an aminobutyric acid-polyethylene glycol (of different chain lengths)-substituted hypocrellin derivative (R.sub.1═R.sub.2═—CH.sub.2CH.sub.2CH.sub.2COO—PEGn, R.sub.3═—COCH.sub.3, R.sub.4═—H): hypocrellin B HB (100 mg, 0.18 mmol), aminobutyric acid (10 mmol), and NaOH (2 g) were dissolved in 100 mL of a mixed solution of DMF and water (at a volume ratio of 1:1), and after fully mixed, a mixed solution was heated to 120° C. under the protection of nitrogen and stirred in a lucifugous condition for a reaction for 10h. After the reaction, dilute hydrochloric acid was added to adjust the pH to weak acidity, and filtration was performed to collect a precipitate. A blue black solid was dissolved in 200 mL of dichloromethane, a solution was washed once with 100 mL of a dilute hydrochloric acid aqueous solution and then washed twice with distilled water, an organic layer was dried by using anhydrous magnesium sulfate, filtered, and spin-dried. Add DCC (200 mg) to the obtained crude product and dissolved in 50 mL of anhydrous dichloromethane, to react with polyethylene glycol methyl esters (HOOC-PEGn-OCH.sub.3, 2 g) of different chain lengths, respectively, and a reaction solution was stirred in a lucifugous condition at room temperature for a reaction for 8h. After the reaction, the reaction solution was added to 100 mL of dichloromethane, a mixed solution was washed once with 100 mL of dilute hydrochloric acid aqueous solution and then washed with distilled water three times, an organic layer was dried by using anhdrous magnesium sulfate and filtered, and the crude product was separated out by means of thin-layer chromatography, with a developing agent being a mixed solution of ethyl acetate:ethanol=5:1, to obtain blue black solid products HB-8a-PEGn, HB-8b-PEGn, HB-8c-PEGn, and HB-8d-PEGn (n=1, 6, 12), respectively. HB-8a-PEG1 (n=): yield: 12.8%, R.sub.f: 0.34; MS(ESI+): 800.3; maximum absorption wavelength: 621 nm molar extinction coefficient: 30.500M.sup.−1cm.sup.−1; singlet oxygen yield: 28%. HB-8b-PEG6 (n=6): yield: 8.9%, R.sub.f: 0.36; MS(ESI+): 1240.6; maximum absorption wavelength: 622 nm; molar extinction coefficient; 30000M.sup.−1cm.sup.−1; singlet oxygen yield: 30%. HB-8c-PEG6 (n=6): yield: 16.4%. R.sub.f: 0.28; MS (ESI+): 1240.6; maximum absorption wavelength: 630 nm; molar extinction coefficient: 32,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 34%. HB-8c-PEG12 (n=12): yield: 15.1%, R.sub.f: 0.18; MS (ESI+): 1768.9; maximum absorption wavelength: 628 nm; molar extinction coefficient: 33,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 36%. HB-8d-PEG6 (n=6): yield: 13.2%, R.sub.f: 0.22; MS (ESI+): 1240.6; maximum absorption wavelength: 620 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. Structural formulas of the above amino-substituted products are as follows:

##STR00046## ##STR00047##

Example 21

[0164] Preparation of a diaminobutyric acid-amino PEG (of different chain lengths)-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—(CH.sub.2).sub.3CO—NH-PEGn, R.sub.3═—COCH.sub.3, R.sub.4═—H) (PEG is polyethylene glycol, n is the number of ethylene glycol units, and n=1, 6, 12): a synthetic route is similar to the preparation of the diaminobutyric acid-substituted polyethylene glycol-modified hypocrellin B derivative in example 20, and four blue black solid products HB-8a-NH-PEGn.

[0165] HB-8b-NH-PEGn. HB-8c-NH-PEGn, and HB-8d-NH-PEGn (n=1, 6, 12) are obtained, respectively. HB-8a-NH-PEG1 (n=1): yield: 10.8%, R.sub.f: 0.34; MS (ESI+): 844.3; maximum absorption wavelength: 621 nm; molar extinction coefficient: 30,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 28%. HB-8b-NH-PEG6 (n=6): yield: 8.0%, R.sub.f: 0.38: MS (ESI+): 1284.6; maximum absorption wavelength: 624 nm; molar extinction coefficient: 31,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 30%. HB-8c-NH-PEG6 (n=6): yield: 15.4%, R.sub.f: 0.29; MS (ESI+): 1284.6 maximum absorption wavelength: 632 nm, molar extinction coefficient: 32,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 35%. HB-8c-NH-PEG12 (n=12): yield: 14.1%, R.sub.f: 0.18; MS (ESI+): 1812.9; maximum absorption wavelength: 628 nm; molar extinction coefficient: 33,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 38%. HB-8d-NH-PEG6 (n=6): yield: 13.5%, R.sub.f: 0.24; MS (ESI+): 1284.6; maximum absorption wavelength: 622 nm; molar extinction coefficient: 31,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 34%. Structural formulas of the above amino-substituted products are as follows:

##STR00048## ##STR00049##

Example 22

[0166] Preparation of a diaminobutyric acid-substituted deacetyl hypocrellin derivative (R.sub.1═R.sub.2═—CH.sub.2(CH.sub.2).sub.2COOH R.sub.3═R.sub.4═—H): a synthetic method is as shown in FIG. 4, deacetyl hypocrellin HC is used as a raw material, the synthetic method is similar to the preparation of the diaminoacetic acid-substituted hypocrellin derivative in example 17, and four blue black solid products HC-8a-HC-8d are obtained, respectively. HC-8a: yield: 5.4%, R.sub.f: 0.30; MS (ESI+): 642.9; maximum absorption wavelength: 620 nm; molar extinction coefficient: 28,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 26%. HC-8b: yield: 5.2%, R.sub.f: 0.34: MS (ESI+): 642.9; maximum absorption wavelength: 622 nm; molar extinction coefficient: 28,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 25%. HC-8c: yield: 16.8%, R.sub.f: 0.24; MS (ESI+): 642.9; maximum absorption wavelength: 618 nm; molar extinction coefficient: 28,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 27%. HC-8d: yield: 4.2%, R.sub.f: 0.25; MS (ESI+): 642.9; maximum absorption wavelength: 623 nm; molar extinction coefficient: 27,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 26%. Structural formulas of the above amino-substituted products are as follows:

##STR00050##

[0167] FIGS. 14(a) and 14(b) show effect diagrams of dark toxicity and phototoxicity of HC-8c to Hela cells. It can be seen that HC-8c containing two carboxyl groups has almost no cytotoxicity when not exposed to light. After exposure to 635 nm light, HC-8c with a concentration range of 200 nM can kill more than 85% of the Hela cells, while in the same condition, the deacetyl hypocrellin HC can kill 50% of the Hela cells, and the commercial photosensitizer hematoporphyrin derivative HpD can kill only about 10% of the Hela cells, indicating that a photodynamic effect of HC-8c is significantly better than that of HC and the commercial photosensitizer hematoporphyrin HpD.

Example 23

[0168] Preparation of an aminobutyric acid-polyethylene glycol (of different chain lengths)-substituted deacetyl hypocrellin derivative (R.sub.1═R.sub.2═—CH.sub.2CH.sub.2CH.sub.2COO—PEGn. R.sub.3═R.sub.4═—H) (PEG is polyethylene glycol, n is the number of ethylene glycol units, and n=1, 6, 12): for a synthetic method, reference is made to the preparation of the diaminobutyric acid-PEG (of different chain lengths)-substituted hypocrellin derivative in example 20, and four blue black solid products HC-8a-PEGn, HC-8b-PEGn, HC-8c-PEGn, and HC-8d-PEGn (n=1, 6, 12) are obtained, respectively. HC-8a-PEG1 (n=1): yield: 11.8%, R.sub.f: 0.32; MS (ESI+): 758.3; maximum absorption wavelength: 621 nm: molar extinction coefficient: 30,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 28%. HC-8b-PEG6 (n=6): yield: 8.6%, R.sub.f: 0.38; MS (ESI+): 1198.6; maximum absorption wavelength: 624 nm; molar extinction coefficient: 30,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HC-8c-PEG6 (n=6): yield: 16.8%, R.sub.f: 0.26; MS (ESI+): 1198.6; maximum absorption wavelength: 632 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 36%. HC-8c-PEG12 (n=12): yield: 15.6%, R.sub.f: 0.20; MS (ESI+): 1726.9; maximum absorption wavelength: 628 nm, molar extinction coefficient: 32,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 38%. HC-8d-PEG6 (n=6): yield: 14.2%, R.sub.f: 0.24; MS (ESI+): 1198.6; maximum absorption wavelength: 624 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. Structural formulas of the above amino-substituted products are as follows:

##STR00051## ##STR00052##

[0169] FIGS. 15(a) and 15(b) show effects of dark toxicity and phototoxicity for killing tumor cells of HC-8c-PEG6. HC-8c-PEG6 has almost no cytotoxicity when not exposed to light. After exposure to 635 nm light, HC-8c-PEG6 with a concentration range of 240 nM can kill more than 90% of the Hela cells, while in the same condition, the deacetyl hypocrellin HC can kill 50% of the Hela cells, indicating that a photodynamic effect of HC-8c-PEG6 is significantly better than that of the deacetyl hypocrellin HC and the commercial photosensitizer hematoporphyrin HpD.

Example 24

[0170] Preparation of an aminobutyric acid-amino PEG (of different chain lengths)-substituted deacetyl hypocrellin B derivative (R.sub.1═R.sub.2═—CH.sub.2(CH.sub.2).sub.4CO—NH-PEGn, R.sub.3═R.sub.4═—H) (PEG is polyethylene glycol, n is the number of ethylene glycol units, and n=1, 6, 12): a synthetic route is similar to the preparation of the aminobutyric acid-polyethylene glycol (of different chain lengths)-substituted deacetyl hypocrellin derivative in example 20, and four blue black solid products HC-8a-NH-PEGn, HC-8b-NH-PEGn, HC-8c-NH-PEGn, and HC-8d-NH-PEGn (n=1, 6, 12) are obtained, respectively. HC-8a-NH-PEG1 (n=1): yield: 12.6%, R.sub.f: 0.32; MS (ESI+): 844.3; maximum absorption wavelength: 621 nm; molar extinction coefficient: 30,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 30%. HC-8b-NH-PEG6 (n=6): yield: 8.5%, R.sub.f: 0.36; MS (ESI+): 1244.6; maximum absorption wavelength: 622 nm; molar extinction coefficient: 30,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 31%. HC-8c-NH-PEG6 (n=6): yield: 17.8%. R.sub.f: 0.28; MS (ESI+): 1244.6: maximum absorption wavelength: 630 nm; molar extinction coefficient: 32.500 M.sup.−1cm.sup.−1; singlet oxygen yield: 35%. HC-8c-NH-PEG12 (n=12): yield: 14.6%, R.sub.f: 0.20; MS (ESI+): 1772.9; maximum absorption wavelength: 628 nm; molar extinction coefficient: 32,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 36%. HC-8d-NH-PEG6 (n=6): yield: 12.2%, R.sub.f: 0.25; MS (ESI+): 1244.6; maximum absorption wavelength: 625 nm; molar extinction coefficient: 31,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. Structural formulas of the above amino-substituted products are as follows:

##STR00053## ##STR00054##

Example 25

[0171] Preparation of an aminobutyric acid-sulfonic acid (of different chain lengths)-substituted deacetyl hypocrellin B derivative (R.sub.1═R.sub.2═—(CH.sub.2).sub.3CO—NH—(CH.sub.2)—SO.sub.3H, R.sub.3═R.sub.4═—H) (n=2, 4, 6): a synthetic route is similar to the preparation of the aminobutyric acid-polyethylene glycol (of different chain lengths)-substituted deacetyl hypocrellin derivative in example 20, and four blue black solid products HC-8a-NH—Cn-SO.sub.3H—HC-8d-NH—Cn-SO.sub.3H (n=2, 4, 6) are obtained, respectively. HC-8a-NH-C2-SO.sub.3H (n=2): yield: 10.6%, R.sub.f: 0.30; MS (ESI+): 856.3; maximum absorption wavelength: 620 nm; molar extinction coefficient: 31,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HC-8b-NH-C4-SO.sub.3H (n=4): yield: 9.5%, R.sub.f: 0.34: MS (ESI+): 912.6; maximum absorption wavelength: 624 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 33%. HC-8c-NH-C6-SO.sub.3H (n=6): yield: 18.8%. R.sub.f: 0.30; MS (ESI+): 968.6; maximum absorption wavelength: 632 nm: molar extinction coefficient: 33,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 35%. HC-8d-NH-C4-SO.sub.3H (n=4): yield: 13.2%, R.sub.f: 0.26; MS (ESI+): 912.6; maximum absorption wavelength: 626 nm: molar extinction coefficient: 30,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. Structural formulas of the above amino-substituted products are as follows:

##STR00055## ##STR00056##

Example 26

[0172] Preparation of an aminobutyric acid-quaternary ammonium salt (of different chain lengths)-substituted deacetyl hypocrellin B derivative (R.sub.1═R.sub.2═—(CH.sub.2).sub.3COO—(CH.sub.2).sub.n—N.sup.+(CH.sub.3).sub.3, R.sub.3═R.sub.4═—H) (n=2, 4, 6): a synthetic route is similar to the preparation of the aminobutyric acid-polyethylene glycol (of different chain lengths)-substituted deacetyl hypocrellin derivative in example 20, and four blue black solid products HC-8a-Cn-N.sup.+-HC-8d-Cn-N.sup.+ (n=2, 4, 6) are obtained, respectively. HC-8a-C.sub.2—N.sup.+ (n=2): yield: 9.6%, R.sub.f: 0.32; MS (ESI+): 814.3; maximum absorption wavelength: 622 nm; molar extinction coefficient: 30,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 28%. HC-8b-C.sub.4—N.sup.+ (n=4): yield: 9.8%, R.sub.f: 0.36; MS (ESI+): 870.6; maximum absorption wavelength: 625 nm; molar extinction coefficient: 31,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HC-8c-C6-N.sup.+ (n=6): yield: 19.8%, R.sub.f: 0.32; MS (ESI+): 926.6; maximum absorption wavelength: 632 nm: molar extinction coefficient: 32,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 35%. HC-8d-C.sub.4—N.sup.+ (n=4): yield: 15.2%, R.sub.f: 0.28: MS (ESI+): 870.6; maximum absorption wavelength: 624 nm; molar extinction coefficient: 31,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 34%. Structural formulas of the above amino-substituted products are as follows:

##STR00057## ##STR00058##

Example 27

[0173] Preparation of an aminobutyric acid-amino quaternary ammonium salt (of different chain lengths)-substituted deacetyl hypocrellin B derivative (R.sub.1═R.sub.2═—(CH.sub.2).sub.3CO—NH—(CH.sub.2)—N.sup.+(CH.sub.3).sub.3, R.sub.3=R═—H) (n=24.6): a synthetic route is similar to the preparation of the aminobutyric acid-polyethylene glycol (of different chain lengths)-substituted deacetyl hypocrellin derivative in example 20, and four blue black solid products HC-8a-NH—Cn-N.sup.+˜HC-8d-NH—Cn-N.sup.+ (n=2, 4, 6) are obtained, respectively. HC-8a-NH—C.sub.2—N.sup.+ (n=2): yield: 8.6%, R.sub.f: 0.32; MS (ESI+): 813.3; maximum absorption wavelength: 622 nm; molar extinction coefficient: 30,000M.sup.−1cm.sup.−1; singlet oxygen yield: 29%. HC-8b-NH-C4-N (n=4): yield: 9.5%, R.sub.f:0.35: MS (ESI+): 869.6; maximum absorption wavelength: 626 nm; molar extinction coefficient: 30,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HC-8c-NH—C.sub.6—N.sup.+ (n=6): yield: 16.8%, R.sub.f: 0.38; MS (ESI+): 925.6; maximum absorption wavelength: 634 nm; molar extinction coefficient: 32,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 36%. HC-8d-NH—C.sub.4—N.sup.+ (n=4): yield: 13.2%, R.sub.f: 0.28; MS (ESI+): 869.6: maximum absorption wavelength: 624 nm; molar extinction coefficient: 30.500 M.sup.−1cm.sup.−1; singlet oxygen yield: 33%. Structural formulas of the above amino-substituted products are as follows:

##STR00059## ##STR00060##

Example 28

[0174] Preparation of an aminocaproic acid-substituted deacetyl hypocrellin derivative (R.sub.1═R.sub.2═—CH(CH.sub.2).sub.4COOH, R.sub.1═R.sub.4═—H): a synthetic route is similar to the preparation of the aminoacetic acid-substituted deacetyl hypocrellin derivative in example 17, and four blue black solid products HC-9a-HC-9d are obtained, respectively. HC-9a: yield: 8.4%. R.sub.f: 0.30: MS (ESI+): 698.5; maximum absorption wavelength: 620 nm: molar extinction coefficient: 26,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 26%. HC-9b: yield: 7.2%, R.sub.f: 0.36; MS (ESI+): 698.6; maximum absorption wavelength: 622 nm; molar extinction coefficient: 27,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 24%. HC-9c: yield: 8.8%, R.sub.f: 0.24; MS (ESI+): 698.5; maximum absorption wavelength: 628 nm: molar extinction coefficient: 27,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 29%. HC-9d: yield: 5.8%, R.sub.f: 0.25; MS (ESI+): 698.9; maximum absorption wavelength: 624 nm; molar extinction coefficient: 25,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 26%. Structural formulas of the above amino-substituted products are as follows:

##STR00061##

Example 29

[0175] Preparation of an aminocaproic acid-PEG (of different chain lengths)-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—CH.sub.2(CH.sub.2).sub.4COO-PEGn, R.sub.3═R.sub.4═—H) (PEG is polyethylene glycol, n is the number of ethylene glycol units, and n=1, 6, 12): a synthetic route is similar to the preparation of the aminobutyric acid-polyethylene glycol (of different chain lengths)-substituted deacetyl hypocrellin derivative in example 20, and four blue black solid products HC-9a-PEGn-HC-9d-PEGn (n=1, 6, 12) are obtained, respectively. HC-9a-PEG1 (n=1): yield: 10.6%, R.sub.f: 0.32; MS (ESI+): 814.3; maximum absorption wavelength: 621 nm; molar extinction coefficient: 30,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HC-9b-PEG6 (n=6): yield: 8.7%, R.sub.f: 0.38; MS (ESI+): 1254.6; maximum absorption wavelength: 622 nm; molar extinction coefficient: 30,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 30%. HC-9c-PEG6 (n=6): yield: 16.8%, R.sub.f: 0.30: MS (ESI+): 1254.6; maximum absorption wavelength: 628 nm; molar extinction coefficient: 33,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 36%. HC-9d-PEG12 (n=12): yield: 11.2%, R.sub.f: 0.25; MS (ESI+): 1782.6; maximum absorption wavelength: 625 nm: molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. Structural formulas of the above amino-substituted products are as follows:

##STR00062## ##STR00063##

Example 30

[0176] Preparation of an aminocaproic acid-quaternary ammonium salt (of different chain lengths)-substituted hypocrellin derivative (R.sub.1═R.sub.2═—(CH.sub.2).sub.5COO—(CH.sub.2)—N.sup.+(CH.sub.3).sub.3, R.sub.3═R.sub.4═—H) (n=2, 4, 6): a synthetic route is similar to the preparation of the aminobutyric acid-polyethylene glycol (of different chain lengths)-substituted deacetyl hypocrellin derivative in example 20, and four blue black solid products HC-9a-Cn-N.sup.+-HC-9d-Cn-N.sup.+ (n=2, 4, 6) are obtained, respectively. HC-9a-C.sub.2—N.sup.+ (n=2): yield: 10.6%, R.sub.f: 0.32; MS (ESI+): 814.3; maximum absorption wavelength: 622 nm; molar extinction coefficient: 30,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 26%. HC-9b-C.sub.4—N.sup.+ (n=4): yield: 9.2%, R.sub.f: 0.38; MS (ESI+): 870.6; maximum absorption wavelength: 625 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HC-9c-C6-N.sup.+ (n=6): yield: 16.8%, R.sub.f: 0.30; MS (ESI+): 926.6; maximum absorption wavelength: 630 nm; molar extinction coefficient: 33,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 36%. HC-9d-C.sub.4—N.sup.+ (n=4): yield: 18.2%. R.sub.f: 0.22; MS (ESI+): 870.6; maximum absorption wavelength: 626 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 33%. Structural formulas of the above amino-substituted products are as follows:

##STR00064## ##STR00065##

Example 31

[0177] Preparation of an aminopropionic acid-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—CHCH.sub.2COOH, R.sub.3=—COCH.sub.3, R.sub.4═—H): a synthetic route is similar to the preparation of the diaminoacetic acid-substituted hypocrellin B derivative in example 17, NH.sub.2—CH.sub.2CH.sub.2COOH is used as a raw material, and four blue black solid products HB-10a-HB-10d are obtained, respectively. HB-10a: yield: 8.4%, R.sub.f: 0.40; MS (ESI+): 656.6; maximum absorption wavelength: 620 nm; molar extinction coefficient: 31,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HB-10b: yield: 6.2%, R.sub.f: 0.32; MS (ESI+): 656.6; maximum absorption wavelength: 615 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 34%. HB-10c: yield: 9.4%, R.sub.f: 0.24; MS (ESI+): 656.6; maximum absorption wavelength: 622 nm; molar extinction coefficient: 33,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 38%. HB-10d: yield: 4.6%, R.sub.f: 0.20; MS (ESI+): 656.6; maximum absorption wavelength: 621 nm; molar extinction coefficient: 32,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%.

[0178] Results of confocal fluorescence imaging experiments shown in FIGS. 6(a) and 6(c) indicate that HB-10a has good biocompatibility and is able to enter a lysosome of a Hela cell and generate an excellent red-light fluorescence image in the cell. Structural formulas of the above amino-substituted products are as follows:

##STR00066##

Example 32

[0179] Preparation of an aminopropionic acid-amino PEG (of different chain lengths)-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—CH.sub.2CHCO—NH-PEGn, R═—COCH.sub.3, R.sub.4═—H) (PEG is polyethylene glycol, n is the number of ethylene glycol units, and n=1, 6, 12): a synthetic route is similar to the preparation of the aminobutyric acid-polyethylene glycol (of different chain lengths)-substituted deacetyl hypocrellin derivative in example 20, and four blue black solid products HB-10a-NH-PEGn, HB-10b-NH-PEGn, HB-10c-NH-PEGn, and HB-10d-NH-PEGn (n=1, 6, 12) are obtained, respectively. HB-10a-NH-PEG1 (n=1): yield: 10.6%, R.sub.f: 0.32; MS (ESI+): 858.3; maximum absorption wavelength: 621 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 30%. HB-10b-NH-PEG6 (n=6): yield: 9.5%, R.sub.f: 0.38; MS (ESI+): 1294.6; maximum absorption wavelength: 622 nm; molar extinction coefficient: 30,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 34%. HB-10c-NH-PEG6 (n=6): yield: 18.8%. R.sub.f: 0.24; MS (ESI+): 1294.6; maximum absorption wavelength: 628 nm; molar extinction coefficient: 32,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 38%. HB-10d-NH-PEG12 (n=12): yield: 13.2%, R.sub.f: 0.25; MS (ESI+): 1822.6; maximum absorption wavelength: 626 nm; molar extinction coefficient: 31,600 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. Structural formulas of the above amino-substituted products are as follows:

##STR00067##

Example 33

[0180] Preparation of an aminosulfonic acid-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—(CH.sub.2).sub.m—SO.sub.3H. R.sub.3═—COCH.sub.3, R.sub.4═—H): a substituted amino raw material is NH.sub.2(CH.sub.2).sub.m—SO.sub.3H (m=2, 4, 6), a synthetic route is similar to the preparation of the aminobutyric acid-polyethylene glycol (of different chain lengths)-substituted deacetyl hypocrellin derivative in example 20, and four blue black solid products HB-11a-C.sub.m—SO.sub.3H, HB-11b-C.sub.m—SO.sub.3H, HB-11c-C.sub.m—SO.sub.3H, and HB-11d-C.sub.m—SO.sub.3H (n=2, 4, 6) are obtained, respectively. HB-1a-C.sub.2—SO.sub.3H (n=2): yield: 8.6%. R.sub.f: 0.30; MS (ESI+): 728.3; maximum absorption wavelength: 620 nm; molar extinction coefficient: 30,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HB-11b-C.sub.4—SO.sub.3H (n=4): yield: 10.5%, R.sub.f: 0.34; MS (ESI+): 784.6; maximum absorption wavelength: 620 nm; molar extinction coefficient: 30,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 34%. HB-11c-C.sub.6—SO.sub.3H (n=6): yield: 16.8%, R.sub.f: 0.30; MS (ESI+): 840.6; maximum absorption wavelength: 626 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 38%. HB-11d-C.sub.4—SO.sub.3H (n=4): yield: 11.2%, R.sub.f: 0.26; MS (ESI+): 784.6; maximum absorption wavelength: 626 nm; molar extinction coefficient: 30,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. Structural formulas of the above amino-substituted products are as follows:

##STR00068##

Example 34

[0181] Preparation of an aminoethyl methanesulfonate-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—CH.sub.2CH.sub.2SO.sub.3CH.sub.3, R.sub.3═—COCH.sub.3, R.sub.4═—H): a substituted amino raw material is NH.sub.2CH.sub.2CHSO.sub.3CH.sub.3, a synthetic route is similar to the preparation of the diaminoacetic acid-substituted hypocrellin B derivative in example 17, and four blue black solid products HB-12a-HB-12d are obtained, respectively. HB-12a: yield: 6.4%, R.sub.f: 0.31; MS (ESI+): 756.7; maximum absorption wavelength: 621 nm; molar extinction coefficient: 23,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 20%. HB-12b: yield: 6.2%, R.sub.f: 0.33; MS (ESI+): 756.7 maximum absorption wavelength: 621 nm; molar extinction coefficient: 22,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 22%. HB-12c: yield: 5.9%, R.sub.f: 0.25; MS (ESI+): 756.7; maximum absorption wavelength: 622 nm; molar extinction coefficient: 23,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 22%. HB-12d: yield: 5.6%, R.sub.f: 0.23; MS (ESI+): 756.7; maximum absorption wavelength: 624 nm; molar extinction coefficient: 21,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 21%. Structural formulas of the above amino-substituted products are as follows:

##STR00069##

Example 35

[0182] Preparation of a 4-tranexamic acid-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—CH.sub.2C.sub.6H.sub.10COOH, R.sub.3═—COCH.sub.3, R.sub.4═—H): a substituted amino raw material is NH.sub.2—CH.sub.2C.sub.6H.sub.10COOH, a synthetic method is similar to the preparation of the diaminoacetic acid-substituted hypocrellin B derivative in example 17, and four blue black solid products HB-13a-HB-13d are obtained, respectively. HB-13a: yield: 7.8%, R.sub.f: 0.36; MS (ESI+): 792.1; maximum absorption wavelength: 621 nm; molar extinction coefficient: 28,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 28%. HB-13b: yield: 8.0%, R.sub.f: 0.39; MS (ESI+): 792.1; maximum absorption wavelength: 621 nm; molar extinction coefficient: 28,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 26%. HB-13c: yield: 5.8%, R.sub.f: 0.36; MS (ESI+): 792.5; maximum absorption wavelength: 621 nm; molar extinction coefficient: 29,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 30%. HB-13d: yield: 5.8%. R.sub.f: 0.39; MS (ESI+): 792.9; maximum absorption wavelength: 621 nm; molar extinction coefficient: 28,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 28%. Structural formulas of the above amino-substituted products are as follows:

##STR00070##

[0183] The above prepared compound HB-13c contains two carboxyl groups of a tranexamic acid, making the photosensitizer molecules have good water solubility in a physiological condition; and each milliliter of normal saline can dissolve more than 10 mg of photosensitizer molecules. Therefore, the photosensitive drug can be well transported in blood vessels during intravenous injection, without causing a vascular blockage. FIGS. 16(a) and 16(b) show effects of dark toxicity and phototoxicity for killing tumor cells of HB-13c. As can be seen from the figures, HB-13c containing two carboxyl groups has almost no cytotoxicity in a non-light condition. After exposure to 635 nm red light, HB-13c with a concentration range of 240 nM can kill more than 85% of the Hela cells, while in the same condition, the hypocrellin B HB can kill 30% of the Hela cells, and the commercial photosensitizer hematoporphyrin derivative HpD can kill only about 10% of the Hela cells, indicating that a photodynamic effect of HB-13c is significantly better than that of the hypocrellin B HB and the commercial photosensitizer hematoporphyrin HpD.

Example 36

[0184] Preparation of a 4-aminomethyl cyclohexanecarboxylate-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—CH.sub.2C.sub.6H.sub.10COOCH.sub.3, R.sub.3═—COCH.sub.3, R.sub.4═—H): a substituted amino raw material is NH.sub.2—CH.sub.2C.sub.6H.sub.1COOCH.sub.3, a synthetic method is similar to the preparation of the diaminoacetic acid-substituted hypocrellin B derivative in example 17, and four blue black solid products HB-13a-AcE-HB-13d-AcE are obtained, respectively. HB-13a-AcE: yield: 5.8%, R.sub.f: 0.38; MS (ESI+): 820.1; maximum absorption wavelength: 625 nm; molar extinction coefficient: 27,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 25%. HB-13b-AcE: yield: 8.5%. R.sub.f: 0.40: MS (ESI+): 820.1: maximum absorption wavelength: 623 nm; molar extinction coefficient: 27.000 M.sup.−1cm.sup.−1; singlet oxygen yield: 24%. HB-13c-AcE: yield: 5.5%, R.sub.f: 0.32: MS (ESI+): 820.8; maximum absorption wavelength: 621 nm; molar extinction coefficient: 28,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 30%. HB-13d-AcE: yield: 5.9%, R.sub.f: 0.45: MS (ESI+): 820.9; maximum absorption wavelength: 623 nm; molar extinction coefficient: 28,000 M.sup.1 cm.sup.−1; singlet oxygen yield: 25%. Structural formulas of the above amino-substituted products are as follows:

##STR00071##

Example 37

[0185] Preparation of a 4-tranexamiic acid-amino PEG (of different chain lengths)-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—CH.sub.2C.sub.6H.sub.10COO-PEGn, R.sub.3═—COCH.sub.3, R.sub.4═—H) (PEG is polyethylene glycol, n is the number of ethylene glycol units, and n=1, 4, 8, 16): a synthetic route is similar to the preparation of the aminobutyric acid-polyethylene glycol (of different chain lengths)-substituted deacetyl hypocrellin derivative in example 20, and four blue black solid products HB-13a-PEGn, HB-13b-PEGn, HB-13c-PEGn, and HB-13d-PEGn (n=1, 4, 8, 16) are obtained, respectively. HB-13a-PEG1 (n=1): yield: 8.6%, R.sub.f: 0.32; MS (ESI+): 908.6: maximum absorption wavelength: 622 nm; molar extinction coefficient: 30.500 M.sup.−1cm.sup.−1; singlet oxygen yield: 30%. HB-13b-PEG4 (n=4): yield: 8.5%, R.sub.f: 0.36; MS (ESI+): 1172.6; maximum absorption wavelength: 622 nm; molar extinction coefficient: 30,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HB-13c-PEG4 (n=4): yield: 18.8%, R.sub.f: 0.31; MS (ESI+): 1172.6; maximum absorption wavelength: 630 nm; molar extinction coefficient: 32,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 38%. HB-13c-PEG8 (n=8): yield: 15.2%, R.sub.f: 0.38; MS (ESI+): 1524.6; maximum absorption wavelength: 628 nm: molar extinction coefficient: 33,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 38%. HB-13d-PEG16 (n=16): yield: 10.2%, R.sub.f: 0.26: MS (ESI+): 2228.6; maximum absorption wavelength: 624 nm: molar extinction coefficient: 30,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 33%. Structural formulas of the above amino-substituted products are as follows:

##STR00072##

[0186] The above prepared compound HB-13c-PEG8 contains two 8-PEG long-chains, making the photosensitizer molecules have very high water solubility in a physiological condition; and each milliliter of normal saline can dissolve more than 20 mg of photosensitizer molecules, presenting excellent water solubility. Therefore, the photosensitive drug can be well transported in blood vessels during intravenous injection, without causing a vascular blockage. FIGS. 17(a) and 17(b) show effects of dark toxicity and phototoxicity for killing tumor cells of HB-13c-PEG8, wherein under irradiation of red light, HB-13c-PEG8 with a concentration range of 160 nM can kill more than 90% of the Hela cells, while in the same condition, the hypocrellin B HB can kill 30% of the Hela cells,

Example 38

[0187] Preparation of a 4-tranexamic acid-amino PEG (of different chain lengths)-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—CH.sub.2C.sub.6H.sub.10CO—NH-PEGn, R.sub.3═—COCH.sub.3, R.sub.4═—H) (PEG is polyethylene glycol, n is the number of ethylene glycol units, and n=1, 4, 8, 16): a synthetic route is similar to the preparation of the aminobutyric acid-polyethylene glycol (of different chain lengths)-substituted deacetyl hypocrellin derivative in example 20, and four blue black solid products HB-13a-NH-PEGn, HB-13b-NH-PEGn, HB-13c-NH-PEGn, and HB-13d-NH-PEGn (n=1, 4, 8, 16) are obtained, respectively. HB-13a-NH-PEG1 (n=1): yield: 8.6%, R.sub.f: 0.30; MS (ESI+): 996.6; maximum absorption wavelength: 624 nm; molar extinction coefficient: 31,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HB-13b-NH-PEG4 (n=4): yield: 8.8%, R.sub.f: 0.38; MS (ESI+): 1258.6; maximum absorption wavelength: 622 nm; molar extinction coefficient: 31,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 34%. HB-13c-NH-PEG8 (n=8): yield: 19.2%, R.sub.f: 0.38: MS (ESI+): 1610.6; maximum absorption wavelength: 630 nm; molar extinction coefficient: 33,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 39%. HB-13d-NH-PEG8 (n=8): yield: 7.8%, R.sub.f: 0.32; MS (ESI+): 1610.6; maximum absorption wavelength: 628 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. Structural formulas of the above amino-substituted products are as follows:

##STR00073##

Example 39

[0188] Preparation of a 4-tranexamic acid-PEG (of different chain lengths)-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—CH.sub.2C.sub.6H.sub.10COO-PEGn-OH, R.sub.3═—COCH.sub.3, R.sub.4═—H) (PEG is polyethylene glycol, n is the number of ethylene glycol units, and n=1, 4, 8): a synthetic route is similar to the preparation of the aminobutyric acid-polyethylene glycol (of different chain lengths)-substituted deacetyl hypocrellin derivative in example 20, and four blue black solid products HB-13a-PEGn-OH, HB-13b-PEGn-OH, HB-13c-PEGn-OH, and HB-13d-PEGn-OHn (n=1, 4, 8) are obtained, respectively. HB-13a-PEG1 (n=1): yield: 7.6%. R.sub.f: 0.32; MS (ESI+): 852.6; maximum absorption wavelength: 624 nm: molar extinction coefficient: 31,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HB-13b-PEG4 (n=4): yield: 8.6%, R.sub.f: 0.36; MS (ESI+): 1116.6; maximum absorption wavelength: 624 nm; molar extinction coefficient: 31,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 34%. HB-13c-PEG8 (n=8): yield: 17.2%, R.sub.f: 0.40; MS (ESI+): 1468.6; maximum absorption wavelength: 630 nm; molar extinction coefficient: 32,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 40%. HB-13d-PEG8 (n=8): yield: 8.2%, R.sub.f: 0.25; MS (ESI+): 1468.6; maximum absorption wavelength: 625 nm; molar extinction coefficient: 32,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 30%. Structural formulas of the above amino-substituted products are as follows:

##STR00074##

Example 40

[0189] Preparation of a 4-tranexamic acid-amino PEG (of different chain lengths)-substituted deacetyl hypocrellin derivative (R.sub.1═R.sub.2═—CH.sub.2C.sub.6H.sub.10 COO-PEGn, R.sub.3═R.sub.4═—H) (PEG is polyethylene glycol, n is the number of ethylene glycol units, and n=1, 4, 8): a synthetic route is similar to the preparation of the aminobutyric acid-polyethylene glycol (of different chain lengths)-substituted deacetyl hypocrellin derivative in example 20, and four blue black solid products HC-13a-PEGn, HC-13b-PEGn, HC-13c-PEGn, and HC-13d-PEGn (n=1, 4, 8) are obtained, respectively. HC-13a-PEG1 (n=1): yield: 9.6%, R.sub.f: 0.30; MS (ESI+): 866.6; maximum absorption wavelength: 622 nm, molar extinction coefficient: 30,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HC-13b-PEG4 (n=4): yield: 8.0%, R.sub.f: 0.35; MS (ESI+): 1130.6; maximum absorption wavelength: 624 nm; molar extinction coefficient: 30,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HC-13c-PEG4 (n=4): yield: 17.8%, R.sub.f: 0.34; MS (ESI+): 1130.6; maximum absorption wavelength: 632 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 36%. HC-13c-PEG8 (n=8): yield: 15.2%, R.sub.f: 0.40; MS (ESI+): 1482.6; maximum absorption wavelength: 628 nm; molar extinction coefficient: 32,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 38%. HC-13d-PEG16 (n=16): yield: 9.2%, R.sub.f: 0.28; MS (ESI+): 2186.6; maximum absorption wavelength: 625 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 30%. Structural formulas of the above amino-substituted products are as follows:

##STR00075##

Example 41

[0190] Preparation of a 4-tranexamic acid-amino PEG (of different chain lengths)-substituted deacetyl hypocrellin B derivative (R.sub.1═R.sub.2═—CH.sub.2C.sub.6H.sub.10CO—NH-PEGn, R.sub.3═R.sub.4═—H) (PEG is polyethylene glycol, n is the number of ethylene glycol units, and n=1, 4, 8): a synthetic route is similar to the preparation of the aminobutyric acid-polyethylene glycol (of different chain lengths)-substituted deacetyl hypocrellin derivative in example 20, and four blue black solid products HC-13a-NH-PEGn, HC-13b-NH-PEGn, HC-13c-NH-PEGn, and HC-13d-NH-PEGn (n=1, 4, 8) are obtained, respectively. HC-13a-NH-PEG1 (n=1): yield: 7.6%, R.sub.f: 0.32; MS (ESI+): 952.6; maximum absorption wavelength: 624 nm; molar extinction coefficient: 30,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HC-13b-NH-PEG4 (n=4): yield: 8.8%, R.sub.f: 0.38; MS (ESI+): 1216.6; maximum absorption wavelength: 624 nm; molar extinction coefficient: 30,000 M.sup.−1cm singlet oxygen yield: 32%. HC-13c-NH-PEG8 (n=8): yield: 17.2%, R.sub.f: 0.36; MS (ESI+): 1568.6: maximum absorption wavelength: 630 nm; molar extinction coefficient: 33,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 38%. HC-13d-NH-PEG8 (n=8): yield: 9.8%, R.sub.f: 0.30; MS (ESI+): 1568.6; maximum absorption wavelength: 625 nm; molar extinction coefficient: 32,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 30%. Structural formulas of the above amino-substituted products are as follows:

##STR00076##

[0191] The compound HC-13c and two 8-PEG chains are connected by an amide bond, to obtain HC-13c-NH-PEG8 (example 41) with dark toxicity and phototoxicity effects of killing tumor cells as shown in FIGS. 18(a) and 18(b). Under red light irradiation. HC-13c-NH-PEG8 with a concentration range of 200 nM can kill more than 85% of the Hela cells, while in the same condition, the hypocrellin B HB can kill 60% of the Hela cells, and the commercial photosensitizer hematoporphyrin derivative HpD can kill only about 10% of the Hela cells. The above-described results indicate that A photodynamic effect of HC-13c-NH-PEG8 is significantly better than that of the deacetyl hypocrellin and commercial photosensitizer hematoporphyrin.

Example 42

[0192] Preparation of a 4-tranexamic acid-quaternary ammonium salt (of different chain lengths)-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—CH.sub.2C.sub.6H.sub.10COO—Cn-N(CH.sub.3).sub.3, R.sub.3═R.sub.4═—H) (n is the number of carbon atoms of the quaternary ammonium salt, and n=2, 4, 6): a synthetic route is similar to the preparation of the aminobutyric acid-polyethylene glycol (of different chain lengths)-substituted deacetyl hypocrellin derivative in example 20, and four blue black solid products HC-13a-Cn-N.sup.+, HC-13b-Cn-N.sup.+, HC-13c-Cn-N.sup.+, and HC-13d-Cn-N.sup.+ (n=2, 4, 6) are obtained, respectively. HC-13a-C.sub.2—N.sup.+ (n=2): yield: 11.6%. R.sub.f: 0.32; MS (ESI+): 922.3; maximum absorption wavelength: 622 nm; molar extinction coefficient: 30,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 30%. HC-13b-C.sub.4—N.sup.+ (n=4): yield: 9.2%, R.sub.f: 0.38; MS (ESI+): 978.6; maximum absorption wavelength: 624 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 36%. HC-13c-C6-N.sup.+ (n=6): yield: 15.8%, R: 0.38; MS (ESI+): 1034.6; maximum absorption wavelength: 630 nm; molar extinction coefficient: 33,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 38%. HC-13d-C.sub.4—N.sup.+ (n=4): yield: 13.2%, R.sub.f: 0.32; MS (ESI+): 978.6; maximum absorption wavelength: 630 nm; molar extinction coefficient: 32,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 33%. Structural formulas of the above amino-substituted products are as follows:

##STR00077##

Example 43

[0193] Preparation of a 4-tranexamic acid-sulfonic acid group (of different chain lengths)-substituted hypocrellin B derivative (R.sub.1═R.sub.2—CH.sub.2C.sub.6H.sub.10CO—NH—Cn-SO.sub.3H, R.sub.3═R.sub.4═—H) (n is the number of carbon atoms of the sulfonate, and n=2, 4, 6): a synthetic route is similar to the preparation of the aminobutyric acid-polyethylene glycol (of different chain lengths)-substituted deacetyl hypocrellin derivative in example 20, and four blue black solid products HC-13a-Cn-SO.sub.3H, HC-13b-Cn-SO.sub.3H, HC-13c-Cn-SO.sub.3H, and HC-13d-Cn-SO.sub.3H (n=2, 4, 6) are obtained, respectively. HC-13a-C.sub.2—SO.sub.3H (n=2): yield: 8.6%, R.sub.f: 0.32: MS (ESI+): 964.3; maximum absorption wavelength: 622 nm; molar extinction coefficient: 31,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HC-13b-C.sub.4—SO.sub.3H (n=4): yield: 11.5%, R.sub.f: 0.34; MS (ESI+): 1020.6; maximum absorption wavelength: 622 nm; molar extinction coefficient: 30,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HC-13c-C6-SO.sub.3H (n=6): yield: 17.8%, R.sub.f: 0.36: MS (ESI+): 1076.6: maximum absorption wavelength: 630 nm; molar extinction coefficient: 33,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 36%. HC-13d-C.sub.4—SO.sub.3H (n=4): yield: 13.2%, R.sub.f: 0.26; MS (ESI+): 1020.6; maximum absorption wavelength: 624 nm; molar extinction coefficient: 31,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 30%. Structural formulas of the above amino-substituted products are as follows:

##STR00078##

Example 44

[0194] Preparation of a 4-tranexamic acid-amino triphenylphosphine (of different chain lengths)-substituted hypocrellin derivative (R.sub.1═R.sub.2═—CH.sub.2C.sub.6H.sub.10CO—NH—Cn-PPh.sub.3.sup.+, R.sub.3═R.sub.4═—H) (n is the number of carbon atoms of the amino triphenylphosphine, and n=2, 4, 6): a synthetic route is similar to the preparation of the aminobutyric acid-polyethylene glycol (of different chain lengths)-substituted deacetyl hypocrellin derivative in example 20, and four blue black solid products HC-13a-NH—Cn-PPh.sub.3.sup.+, HC-13b-NH—Cn-PPh.sub.3.sup.+, HC-13c-NH—Cn-PPh.sub.3.sup.+, and HC-13d-NH—Cn-PPh.sub.3.sup.+ (n=2, 4, 6) are obtained, respectively. HC-13a-NH—C.sub.2—PPh.sub.3.sup.+ (n=2): yield: 9.6%, R.sub.f: 0.32; MS (ESI+): 1327.3; maximum absorption wavelength: 622 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HC-13b-NH—C.sub.4—PPh.sub.3+(n=4): yield: 10.5%, R.sub.f: 0.38; MS (ESI+): 1383.6; maximum absorption wavelength: 626 nm; molar extinction coefficient: 30,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 34%. HC-13c-NH—C.sub.6—PPh.sub.3.sup.+ (n=6): yield: 18.8%. R.sub.f: 0.40; MS (ESI+): 1439.6; maximum absorption wavelength: 632 nm: molar extinction coefficient: 33,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 38%. HC-13d-NH—C.sub.4—PPh.sub.3.sup.+ (n=4): yield: 12.2%, R.sub.f: 0.26; MS (ESI+): 1383.6; maximum absorption wavelength: 618 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 30%. Structural formulas of the above amino-substituted products are as follows:

##STR00079##

Example 45

[0195] Preparation of a 4-aminocyclohexanecarboxylic acid-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—C.sub.6H.sub.10COOCH.sub.3, R.sub.3═—COCH.sub.3, R.sub.4═—H): a substituted amino raw material is NH.sub.2—C.sub.6H.sub.10COOH, a synthetic method is similar to the preparation of the diaminoacetic acid-substituted hypocrellin B derivative in example 17, and four blue black solid products HB-14a-HB-14d are obtained, respectively. HB-14a: yield: 5.8%. R.sub.f: 0.38; MS (ESI+): 764.1; maximum absorption wavelength: 625 nm; molar extinction coefficient: 27,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 25%. HB-14b: yield: 8.5%, R.sub.f: 0.40; MS (ESI+): 764.1; maximum absorption wavelength: 623 nm; molar extinction coefficient: 27,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 24%. HB-14c: yield: 5.5%, R.sub.f: 0.32; MS (ESI+): 764.8; maximum absorption wavelength: 621 nm; molar extinction coefficient: 28,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 26%. HB-14d: yield: 5.9%, R.sub.f: 0.45: MS (ESI+): 764.9; maximum absorption wavelength: 623 nm, molar extinction coefficient: 27,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 24%. Structural formulas of the above amino-substituted products are as follows:

##STR00080##

Example 46

[0196] Preparation of a 4-aminocyclohexanecarboxylic acid-amino PEG (of different chain lengths)-substituted deacetyl hypocrellin B derivative (R.sub.1═R.sub.2═—C.sub.6H.sub.10COO-PEGn, R.sub.3═—COCH.sub.3, R.sub.4═—H) (PEG is polyethylene glycol, n is the number of ethylene glycol units, and n=1, 6, 12): a synthetic route is similar to the preparation of the aminobutyric acid-polyethylene glycol (of different chain lengths)-substituted deacetyl hypocrellin derivative in example 20, and four blue black solid products HB-14a-PEGn, HB-14b-PEGn, HB-14c-PEGn, and HB-14d-PEGn (n=1, 6, 12) are obtained, respectively. HB-14a-PEG1 (n=): yield: 9.6%. R.sub.f: 0.32; MS (ESI+): 880.6; maximum absorption wavelength: 622 nm; molar extinction coefficient: 30,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HB-14b-PEG6 (n=6): yield: 8.2%, R: 0.36; MS (ESI+): 1320.6; maximum absorption wavelength: 624 nm, molar extinction coefficient: 30,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 34%. HB-14c-PEG12 (n=12): yield: 17.2%, R.sub.f: 0.42; MS (ESI+): 1848.6; maximum absorption wavelength: 628 nm; molar extinction coefficient: 33,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 38%. HB-14d-PEG6 (n=6): yield: 9.4%, R.sub.f: 0.30: MS (ESI+): 1320.6; maximum absorption wavelength: 630 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. Structural formulas of the above amino-substituted products are as follows:

##STR00081##

Example 47

[0197] Preparation of a 3-aminocyclohexanecarboxylic acid-substituted hypocrellin derivative (R.sub.1═R.sub.2═—C.sub.6H.sub.10COOCH.sub.3, R.sub.3═—COCH.sub.3, R.sub.4═—H): a substituted amino raw material is NH.sub.2—C.sub.7H.sub.10COOH, a synthetic method is similar to the preparation of the diaminoacetic acid-substituted hypocrellin B derivative in example 17, and four blue black solid products HB-15a-HB-15d are obtained, respectively. HB-15a: yield: 5.8%, R.sub.f: 0.38; MS (ESI+): 764.1; maximum absorption wavelength: 625 nm; molar extinction coefficient: 27,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 25%. HB-15b: yield: 8.5%, R.sub.f: 0.40; MS (ESI+): 764.1; maximum absorption wavelength: 623 nm; molar extinction coefficient: 27.000 M.sup.−1cm.sup.−1; singlet oxygen yield: 24%. HB-15c: yield: 5.5%, R.sub.f: 0.32; MS (ESI+): 764.8; maximum absorption wavelength: 621 nm; molar extinction coefficient: 28,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 26%. HB-15d: yield: 5.9%, R.sub.f: 0.45; MS (ESI+): 764.9; maximum absorption wavelength: 623 nm, molar extinction coefficient: 27,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 24%. Structural formulas of the above amino-substituted products are as follows:

##STR00082##

Example 48

[0198] Preparation of a 3-aminocyclohexanecarboxylic acid-PEG (of different chain lengths)-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—CH.sub.10COO-PEGn, R.sub.3═—COCH.sub.3, R.sub.4═—H) (PEG is polyethylene glycol, n is the number of ethylene glycol units, and n=1, 6, 12): a synthetic route is similar to the preparation of the aminobutyric acid-polyethylene glycol (of different chain lengths)-substituted deacetyl hypocrellin derivative in example 20, and four blue black solid products HB-15a-PEGn, HB-15b-PEGn, HB-15c-PEGn, and HB-15d-PEGn (n=1, 6, 12) are obtained, respectively. HB-15a-PEG1 (n=1): yield: 9.8%, R.sub.f: 0.28; MS (ESI+): 880.6; maximum absorption wavelength: 624 nm; molar extinction coefficient: 30,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 30%. HB-15b-PEG6 (n=6): yield: 8.2%, R.sub.f: 0.34; MS (ESI+): 1320.6; maximum absorption wavelength: 624 nm; molar extinction coefficient: 30,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 34%. HB-15c-PEG12 (n=12): yield: 18.2%. R.sub.f: 0.42; MS (ESI+): 1848.6; maximum absorption wavelength: 630 nm: molar extinction coefficient: 33,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 35%. HB-15d-PEG6 (n=6): yield: 9.9%, R.sub.f: 0.32: MS (ESI+): 1320.6; maximum absorption wavelength: 630 nm; molar extinction coefficient: 32,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. Structural formulas of the above amino-substituted products are as follows:

##STR00083## ##STR00084##

Example 49

[0199] Preparation of a 2-aminocyclohexanecarboxylic acid-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—C.sub.6H.sub.10COOCH.sub.3, R.sub.3═—COCH.sub.3, R.sub.4═—H): a substituted amino raw material is NH.sub.2—CH.sub.1COOH a synthetic method is similar to the preparation of the diaminoacetic acid-substituted hypocrellin B derivative in example 17, and four blue black solid products HB-16a-HB-16d are obtained, respectively. HB-16a: yield: 4.8%, R.sub.f: 0.36; MS (ESI+): 764.1; maximum absorption wavelength: 620 nm; molar extinction coefficient: 25,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 25%. HB-16b: yield: 5.5%, R.sub.f: 0.38; MS (ESI+): 764.1; maximum absorption wavelength: 625 nm; molar extinction coefficient: 26,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 26%. HB-16c: yield: 5.5%, R.sub.f: 0.34; MS (ESI+): 764.8; maximum absorption wavelength: 628 nm; molar extinction coefficient: 28,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 28%. HB-16d: yield: 6.9%, R.sub.f: 0.40; MS (ESI+): 764.9; maximum absorption wavelength: 625 nm; molar extinction coefficient: 26,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 26%. Structural formulas of the above amino-substituted products are as follows:

##STR00085##

Example 50

[0200] Preparation of a 2-aminocyclohexanecarboxylic acid-PEG (of different chain lengths)-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—C.sub.6H.sub.10CO—NH-PEGn, R.sub.3═—COCH.sub.3, R.sub.4═—H) (PEG is polyethylene glycol, n is the number of ethylene glycol units, and n=1, 6, 12): a synthetic route is similar to the preparation of the aminobutyric acid-polyethylene glycol (of different chain lengths)-substituted deacetyl hypocrellin derivative in example 20, and four blue black solid products HB-16a-PEGn, HB-16b-PEGn, HB-16c-PEGn, and HB-16d-PEGn (n=1, 6, 12) are obtained, respectively. HB-16a-PEG1 (n=1): yield: 7.8%, R.sub.f: 0.32: MS (ESI+): 880.6: maximum absorption wavelength: 624 nm; molar extinction coefficient: 30.500 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HB-16b-PEG6 (n=6): yield: 8.5%, R.sub.f: 0.34: MS (ESI+): 1320.6; maximum absorption wavelength: 624 nm; molar extinction coefficient: 30,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HB-16c-PEG12 (n=12): yield: 16.2%, R.sub.f: 0.40; MS (ESI+): 1848.6; maximum absorption wavelength: 628 nm; molar extinction coefficient: 33,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 36%. HB-16d-PEG6 (n=6): yield: 9.9%, R.sub.f: 0.34; MS (ESI+): 1320.6; maximum absorption wavelength: 628 nm: molar extinction coefficient: 32,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 33%. Structural formulas of the above amino-substituted products are as follows:

##STR00086## ##STR00087##

Example 51

[0201] Preparation of a 2-aminocyclohexanecarboxylic acid-amino PEG (of different chain lengths)-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—C.sub.6H.sub.10CO—NH-PEGn, R.sub.3═—COCH.sub.3, R.sub.4═—H) (PEG is polyethylene glycol, n is the number of ethylene glycol units, and n=1, 6, 12): a synthetic route is similar to the preparation of the aminobutyric acid-polyethylene glycol (of different chain lengths)-substituted deacetyl hypocrellin derivative in example 20, and four blue black solid products HB-16a-NH-PEGn, HB-16b-NH-PEGn, HB-16c-NH-PEGn, and HB-16d-NH-PEGn (n=1, 6, 12) are obtained, respectively. HB-16a-NH-PEG1 (n=1): yield: 8.8%, R.sub.f: 0.30; MS (ESI+): 925.6; maximum absorption wavelength: 622 nm: molar extinction coefficient: 30000M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HB-16b-NH-PEG6 (n=6): yield: 7.5% R.sub.f: 0.32; MS(ESI+): 1365.6; maximum absorption wavelength: 622 nm; molar extinction coefficient: 31,500M.sup.−1cm.sup.−1; singlet oxygen yield 34%. HB-16c-NH-PEG12 (n=12): yield 18.2%, R.sub.f:0.38; MS(ESI+): 1893.6; maximum absorption wavelength: 628 nm; molar extinction coefficient: 33,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 38%. HB-16d-NH-PEG6 (n=6): yield: 9.5%, R.sub.f: 0.36; MS (ESI+): 1365.6; maximum absorption wavelength: 628 nm; molar extinction coefficient: 32,500M.sup.−1cm.sup.−1; singlet oxygen yield 32%. Structural formulas of the above amino-substituted products are as follows;

##STR00088##

Example 52

[0202] Preparation of a 4-hydroxylcyclohexylamine-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—C.sub.6H.sub.10OH, R.sub.3═—COCH.sub.3, R.sub.4═—H): a substituted amino raw material is NH.sub.2—C.sub.6H.sub.10OH, a synthetic method is similar to the preparation of the di-2-(2-aminoethoxy)ethanol-substituted hypocrellin B derivative in example 2, and four blue black solid products HB-7a-HB-17d are obtained, respectively. HB-17a: yield: 6.9%, R.sub.f: 0.36; MS(ESI+): 708.3; maximum absorption wavelength: 617 nm; molar extinction coefficient: 25,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 22%. HB-17b: yield: 5.2%, R.sub.f: 0.28; MS (ESI+): 708.3; maximum absorption wavelength: 620 nm; molar extinction coefficient: 25,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 20%. HB-17c: yield: 6.9%, R.sub.f: 0.35; MS (ESI+): 708.3; maximum absorption wavelength: 622 nm; molar extinction coefficient: 26,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 23%. HB-17d: yield: 5.7%, R.sub.f: 0.33; MS (ESI+): 708.3; maximum absorption wavelength: 624 nm; molar extinction coefficient: 25,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 22%. Structural formulas of the above amino-substituted products are as follows:

##STR00089##

Example 53

[0203] Preparation of a 4-aminocyclohexanol-carboxyl PEG (of different chain lengths)-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—C.sub.6H.sub.10O—CO-PEGn, R.sub.3═—COCH.sub.3, R.sub.4═—H) (PEG is polyethylene glycol, n is the number of ethylene glycol units, and n=1, 6, 12): a synthetic route is similar to the preparation of the aminobutyric acid-polyethylene glycol (of different chain lengths)-substituted deacetyl hypocrellin derivative in example 20, and four blue black solid products HB-17a-PEGn, HB-17b-PEGn, HB-17c-PEGn, HB-17d-PEGn (n=1, 6, 12) are obtained, respectively. HB-17a-PEG1 (n=1): yield: 7.6%. R.sub.f: 0.32; MS (ESI+): 968.6; maximum absorption wavelength: 622 nm: molar extinction coefficient: 30,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 30%. HB-17b-PEG6 (n=6): yield: 8.5%, R.sub.f: 0.34: MS (ESI+): 1408.6; maximum absorption wavelength: 622 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HB-17c-PEG12 (n=12): yield: 19.2%, R.sub.f: 0.38; MS (ESI+): 1936.6; maximum absorption wavelength: 632 nm: molar extinction coefficient: 33,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 38%. HB-17d-PEG6 (n=6): yield: 8.5%, R.sub.f: 0.32: MS (ESI+): 1408.6; maximum absorption wavelength: 632 nm: molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. Structural formulas of the above amino-substituted products are as follows:

##STR00090## ##STR00091##

Example 54

[0204] Preparation of a 4-aminoethylcyclohexanol-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—CH.sub.2CH.sub.2C.sub.6H.sub.9(OH), R.sub.3═—COCH.sub.3, R.sub.4═—H): a substituted amino raw material is NH.sub.2—CH.sub.2CH.sub.2C.sub.6H.sub.9(OH), a synthetic method is similar to the preparation of the di-2-(2-aminoethoxy)ethanol-substituted hypocrellin B derivative in example 2, and four blue black solid products HB-18a-HB-18d are obtained, respectively. HB-18a: yield: 6.1%, R.sub.f: 0.37; MS (ESI+): 764.2; maximum absorption wavelength: 614 nm; molar extinction coefficient: 24,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 20%. HB-18b: yield: 6.8%, R.sub.f: 0.33; MS (ESI+): 764.2; maximum absorption wavelength: 621 nm: molar extinction coefficient: 24,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 21%. HB-18c: yield: 9.8%, R.sub.f: 0.35; MS (ESI+): 764.2; maximum absorption wavelength: 624 nm; molar extinction coefficient: 23,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 23%; HC-18d: yield: 4.8%, R.sub.f: 0.28; MS (ESI+): 764.2; maximum absorption wavelength: 622 nm; molar extinction coefficient: 23,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 20%; Structural formulas of the above amino-substituted products are as follows:

##STR00092##

Example 55

[0205] Preparation of a 3-aminocyclopentanecarboxylic acid-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—C.sub.5H.sub.8COOH, R.sub.3═—COCH.sub.3, R.sub.4═—H): a synthetic method is similar to the preparation of the di-2-(2-aminoethoxy)ethanol-substituted hypocrellin B derivative in example 2, and four blue black solid products HB-19a-HB-19d are obtained, respectively. HB-19a: yield: 8.0%, R: 0.38: MS (ESI+): 736.2; maximum absorption wavelength: 621 nm; molar extinction coefficient: 25,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 21%. HB-19b: yield: 7.0%, R.sub.f:0.33; MS (ESI+): 736.2; maximum absorption wavelength: 620 nm; molar extinction coefficient: 25,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 22%. HB-19c: yield: 6.7%, R.sub.f:0.30; MS (ESI+): 736.2; maximum absorption wavelength: 622 nm; molar extinction coefficient: 25,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 24%. HB-19d: yield: 5.9%, R.sub.f: 0.38; MS (ESI+): 736.2; maximum absorption wavelength: 625 nm; molar extinction coefficient: 24,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 20%. Structural formulas of the above amino-substituted products are as follows:

##STR00093##

Example 56

[0206] Preparation of a 3-aminocyclopentanecarboxylic acid-PEG (of different chain lengths)-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—C.sub.5H.sub.8COO-PEGn, R.sub.3═—COCH.sub.3, R.sub.4═—H) (PEG is polyethylene glycol, n is the number of ethylene glycol units, and n=1, 6, 12): a synthetic route is similar to the preparation of the aminobutyric acid-polyethylene glycol (of different chain lengths)-substituted deacetyl hypocrellin derivative in example 20, and four blue black solid products HB-19a-PEGn, HB-19b-PEGn, HB-19c-PEGn, HB-19d-PEGn (n=1, 6, 12) are obtained, respectively. HB-19a-PEG1 (n=1): yield: 7.5%, R.sub.f: 0.32; MS (ESI+): 852.6; maximum absorption wavelength: 624 nm; molar extinction coefficient: 30,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HB-19b-PEG6 (n=6): yield: 9.5%, R.sub.f: 0.36; MS (ESI+): 1292.6; maximum absorption wavelength: 624 nm; molar extinction coefficient: 30,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 34%. HB-19c-PEG12 (n=12): yield: 18.2%, R.sub.f: 0.40; MS (ESI+): 1820.6; maximum absorption wavelength: 636 nm: molar extinction coefficient: 33,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 38%. HB-19d-PEG6 (n=6): yield: 8.4%, R.sub.f: 0.34: MS (ESI+): 1292.6; maximum absorption wavelength: 636 nm: molar extinction coefficient: 31,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. Structural formulas of the above amino-substituted products are as follows:

##STR00094##

Example 57

[0207] Preparation of a 3-aminocyclopentanecarboxylic acid-amino PEG (of different chain lengths)-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—C.sub.5H.sub.8CO—NH-PEGn, R.sub.3═—COCH.sub.3, R.sub.4═—H) (PEG is polyethylene glycol, n is the number of ethylene glycol units, and n=1, 6, 12): a synthetic route is similar to the preparation of the aminobutyric acid-polyethylene glycol (of different chain lengths)-substituted deacetyl hypocrellin derivative in example 20, and four blue black solid products HB-19a-NH-PEGn, HB-19b-NH-PEGn, HB-19c-NH-PEGn. HB-19d-NH-PEGn (n=1, 6, 12) are obtained, respectively. HB-19a-NH-PEG1 (n=1): yield: 8.5%, R.sub.f: 0.30; MS (ESI+): 896.6; maximum absorption wavelength: 622 nm; molar extinction coefficient: 30,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HB-19b-NH-PEG6 (n=6): yield: 10.5%, R.sub.f: 0.38; MS (ESI+): 1336.6; maximum absorption wavelength: 622 nm; molar extinction coefficient: 30,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 30%. HB-19c-NH-PEG12 (n=12): yield: 19.2%, R.sub.f: 0.42; MS (ESI+): 1864.6; maximum absorption wavelength: 630 nm; molar extinction coefficient: 32,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 36%. HB-19d-NH-PEG6 (n=6): yield: 6.4%, R.sub.f: 0.36; MS (ESI+): 1336.6; maximum absorption wavelength: 630 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. Structural formulas of the above amino-substituted products are as follows:

##STR00095## ##STR00096##

Example 58

[0208] Preparation of a 3-aminocyclopentanol-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—C.sub.5H.sub.8OH, R.sub.3═—COCH.sub.3, R.sub.4═—H): a synthetic method is similar to the preparation of the di-2-(2-aminoethoxy)ethanol-substituted hypocrellin B derivative in example 2, and four blue black solid products HB-20a-HB-20d are obtained, respectively. HB-20a: yield: 5.0%. R.sub.f: 0.32; MS (ESI+): 680.2; maximum absorption wavelength: 624 nm; molar extinction coefficient: 24,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 20%. HB-20b: yield: 5.0%, R.sub.f: 0.32; MS (ESI+): 680.2; maximum absorption wavelength: 620 nm; molar extinction coefficient: 24,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 22%. HB-20c: yield: 12.7%, R.sub.f: 0.30: MS (ESI+): 680.2; maximum absorption wavelength: 622 nm: molar extinction coefficient: 24,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 24%.

[0209] HB-20d: yield: 3.9%, R.sub.f: 0.35: MS (ESI+): 680.2; maximum absorption wavelength: 625 nm; molar extinction coefficient: 24,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 20%. Structural formulas of the above amino-substituted products are as follows:

##STR00097##

Example 59

[0210] Preparation of a 3-aminocyclopentanol-carboxyl PEG (of different chain lengths)-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—C.sub.5H.sub.8—O—CO-PEGn, R.sub.3═—COCH.sub.3, R.sub.4═—H) (PEG is polyethylene glycol, n is the number of ethylene glycol units, and n=1, 6, 12): a synthetic route is similar to the preparation of the aminobutyric acid-polyethylene glycol (of different chain lengths)-substituted deacetyl hypocrellin derivative in example 20, and four blue black solid products HB-20a-PEGn, HB-20b-PEGn, HB-20c-PEGn, HB-20d-PEGn (n=1, 6, 12) are obtained, respectively. HB-20a-PEG1 (n=1): yield: 8.1%, R.sub.f: 0.32; MS (ESI+): 940.6; maximum absorption wavelength: 624 nm; molar extinction coefficient: 31,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 30%. HB-20b-PEG6 (n=6): yield: 9.5%, R.sub.f: 0.38; MS (ESI+): 1380.6; maximum absorption wavelength: 624 nm: molar extinction coefficient: 31,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 28%. HB-20c-PEG12 (n=12): yield: 18.2%, R.sub.f: 0.40; MS (ESI+): 1908.6; maximum absorption wavelength: 632 nm; molar extinction coefficient: 32,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 34%. HB-20d-PEG6 (n=6): yield: 6.2%, R.sub.f: 0.32; MS (ESI+): 1380.6; maximum absorption wavelength: 630 nm; molar extinction coefficient: 30,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. Structural formulas of the above amino-substituted products are as follows:

##STR00098## ##STR00099##

Example 60

[0211] Preparation of a 2-aminocyclocarboxylic acid-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—C.sub.5H.sub.8COOH, R.sub.3═—COCH.sub.3. R═—H): a synthetic method is similar to the preparation of the di-2-(2-aminoethoxy)ethanol-substituted hypocrellin B derivative in example 2, and four blue black solid products HB-21a-HB-21d are obtained, respectively. HB-21a: yield: 4.0%. R.sub.f: 0.34; MS (ESI+): 736.2; maximum absorption wavelength: 620 nm; molar extinction coefficient: 23,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 20%. HB-20b: yield: 5.0%, R.sub.f: 0.35; MS (ESI+): 736.2; maximum absorption wavelength: 622 nm: molar extinction coefficient: 23,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 22%. HB-20c: yield: 12.7%, R.sub.f: 0.32: MS (ESI+): 736.2; maximum absorption wavelength: 624 nm; molar extinction coefficient: 24.000 M.sup.−1cm.sup.−1; singlet oxygen yield: 24%. HB-20d: yield: 3.9%, R.sub.f: 0.35: MS (ESI+): 736.2; maximum absorption wavelength: 620 nm: molar extinction coefficient: 23,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 20%. Structural formulas of the above amino-substituted products are as follows:

##STR00100##

Example 61

[0212] Preparation of a 2-aminocyclopentanecarboxylic acid-PEG (of different chain lengths)-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—C.sub.5H.sub.8—COO-PEGn, Rt=—COCH.sub.3, R.sub.4═—H) (PEG is polyethylene glycol, n is the number of ethylene glycol units, and n=1, 6, 12): a synthetic route is similar to the preparation of the aminobutyric acid-polyethylene glycol (of different chain lengths)-substituted deacetyl hypocrellin derivative in example 20, and four blue black solid products HB-21a-PEGn, HB-21b-PEGn, HB-21c-PEGn, HB-21d-PEGn (n=1, 6, 12) are obtained, respectively. HB-21a-PEG1 (n=1); yield: 8.4%. R.sub.f: 0.30; MS (ESI+): 940.6; maximum absorption wavelength: 624 nm: molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 30%. HB-21b-PEG6 (n=6): yield: 9.4%, R.sub.f: 0.34; MS (ESI+): 1380.6; maximum absorption wavelength: 624 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 30%. HB-21c-PEG12 (n=12): yield: 17.2%, R.sub.f: 0.38; MS (ESI+): 1908.6; maximum absorption wavelength: 628 nm; molar extinction coefficient: 33,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 38%. HB-21d-PEG6 (n=6): yield: 7.2%, R.sub.f: 0.32: MS (ESI+): 1380.6; maximum absorption wavelength: 628 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. Structural formulas of the above amino-substituted products are as follows:

##STR00101##

Example 62

[0213] Preparation of a 2-aminocyclocarboxylic acid-amino PEG (of different chain lengths)-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—C.sub.5H.sub.8—COO-PEGn, R.sub.3═—COCH.sub.3, R.sub.4═—H) (PEG is polyethylene glycol, n is the number of ethylene glycol units, and n=1, 6, 12): a synthetic route is similar to the preparation of the aminobutyric acid-polyethylene glycol (of different chain lengths)-substituted deacetyl hypocrellin derivative in example 20, and four blue black solid products HB-21a-NH-PEGn, HB-21b-NH-PEGn, HB-21c-NH-PEGn, HB-21d-NH-PEGn (n=1, 6, 12) are obtained, respectively. HB-21a-NH-PEG1 (n=1): yield: 7.0%, R.sub.f: 0.31; MS (ESI+): 940.6; maximum absorption wavelength: 622 nm: molar extinction coefficient: 31,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 31%. HB-21b-NH-PEG6 (n=6): yield: 8.4%, R.sub.f: 0.35; MS (ESI+): 1380.6; maximum absorption wavelength: 622 nm; molar extinction coefficient: 31,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HB-21c-NH-PEG12 (n=12): yield: 18.2%, R.sub.f: 0.40: MS (ESI+): 1908.6; maximum absorption wavelength: 627 nm; molar extinction coefficient: 33,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 37%. HB-21d-NH-PEG6 (n=6): yield: 7.5%. R.sub.f: 0.32: MS (ESI+): 1380.6; maximum absorption wavelength: 628 nm; molar extinction coefficient: 31,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. Structural formulas of the above amino-substituted products are as follows:

##STR00102## ##STR00103##

Example 63

[0214] Preparation of a di-valine-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—CH(CH(CH.sub.3).sub.2)—COOH, R.sub.3═—COCH.sub.3, R.sub.4═—H): a substituted amino raw material is valine, a synthetic method is similar to the preparation of the diaminoacetic acid-substituted hypocrellin B derivative in example 17, and four blue black solid products HB-22a-HB-22d are obtained, respectively. HB-22a: yield: 7.2%, R.sub.f: 0.38; MS (ESI+): 712.2; maximum absorption wavelength: 621 nm: molar extinction coefficient: 21,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 18%. HB-22b: yield: 6.1%, R.sub.f: 0.30: MS (ESI+): 712.2; maximum absorption wavelength: 618 nm; molar extinction coefficient: 21,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 19%. HB-22c: yield: 5.6%, R.sub.f: 0.28; MS (ESI+): 712.2; maximum absorption wavelength: 620 nm; molar extinction coefficient: 21,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 22%. HB-22d: yield: 5.9%, R.sub.f: 0.26; MS (ESI+): 712.2; maximum absorption wavelength: 625 nm; molar extinction coefficient: 21,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 22%. Structural formulas of the above amino-substituted products are as follows:

##STR00104##

Example 64

[0215] Preparation of a di-serine-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—CH(CH.sub.2OH)—COOH, R.sub.3═—COCH.sub.3, R.sub.4═—H): an amino raw material is serine, a synthetic method is similar to the preparation of the diaminoacetic acid-substituted hypocrellin B derivative in example 17, and four blue black solid products HB-23a-HB-23d are obtained, respectively. HB-23a: yield: 7.4%, R.sub.f: 0.37: MS (ESI+): 688.1; maximum absorption wavelength: 625 nm; molar extinction coefficient: 21,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 20%. HB-23b: yield: 4.2%, R: 0.33; MS (ESI+): 688.1; maximum absorption wavelength: 621 nm; molar extinction coefficient: 21,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 18%. HB-23c: yield: 7.9%, R.sub.f: 0.35; MS (ESI+): 688.1; maximum absorption wavelength: 622 nm; molar extinction coefficient: 22,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 22%. HB-23d: yield: 6.6%, R.sub.f: 0.28; MS (ESI+): 688.1; maximum absorption wavelength: 621 nm; molar extinction coefficient: 21,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 18%. Structural formulas of the above amino-substituted products are as follows:

##STR00105##

Example 65

[0216] Preparation of a serine methylester-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—CH(CH.sub.2OH)—COOCH.sub.3, R.sub.3═—COCH.sub.3, R.sub.4═—H): a substituted amino raw material is serine methylester, a synthetic method is similar to the preparation of the diaminoacetic acid-substituted hypocrellin B derivative in example 17, and four blue black solid products HB-24a-HB-24d are obtained, respectively. HB-24a: yield: 7.4%, R.sub.f: 0.37: MS (ESI+): 716.1: maximum absorption wavelength; 625 nm; molar extinction coefficient: 20500 M.sup.−1cm.sup.−1; singlet oxygen yield: 20%. HB-24b: yield: 4.2%, R.sub.f: 0.33: MS (ESI+): 716.1; maximum absorption wavelength: 621 nm; molar extinction coefficient: 21,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 20%. HB-24c: yield: 7.9%, R.sub.f: 0.35; MS (ESI+): 716.1; maximum absorption wavelength: 622 nm; molar extinction coefficient: 21,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 22%. HB-24d: yield: 6.6%, R.sub.f: 0.28; MS (ESI+): 716.1; maximum absorption wavelength: 621 nm; molar extinction coefficient: 20500 M.sup.−1cm.sup.−1; singlet oxygen yield: 20%. Structural formulas of the above amino-substituted products are as follows:

##STR00106##

Example 66

[0217] Preparation of a cysteine-substituted deacetyl hypocrellin derivative (R.sub.1═R.sub.2═—CH(CH.sub.2SH)—COOH, R.sub.3═—COCH.sub.3, R.sub.4═—H): a substituted amino raw material is cysteine, a synthetic method is similar to the preparation of the diaminoacetic acid-substituted hypocrellin B derivative in example 17, and four blue black solid products HB-25a-HB-25d are obtained, respectively. HB-25a: yield: 6.2%, R.sub.f:0.36; MS (ESI+): 720.0; maximum absorption wavelength: 625 nm; molar extinction coefficient: 21,500M.sup.−1cm.sup.−1; singlet oxygen yield: 18%. HB-25b: yield: 7.8%, R.sub.f: 0.34; MS (ESI+): 720.0; maximum absorption wavelength: 629 nm; molar extinction coefficient: 20,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 20%. HB-25c: yield: 8.7%, R.sub.f: 0.33; MS (ESI+): 720.0; maximum absorption wavelength: 632 nm; molar extinction coefficient: 21,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 20%. HB-25d: yield: 4.9%, R.sub.f:0.40: MS (ESI+): 720.0; maximum absorption wavelength: 630 nm; molar extinction coefficient: 20,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 18%. Structural formulas of the above amino-substituted products are as follows:

##STR00107##

Example 67

[0218] Preparation of an asparagine-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—CH(CH.sub.2CONH.sub.2)—COOH, R.sub.3═—COCH.sub.3, R.sub.4═—H): a substituted amino raw material is asparagine, a synthetic method is similar to the preparation of the diaminoacetic acid-substituted hypocrellin B derivative in example 17, and four blue black solid products HB-26a-HB-26d are obtained, respectively. HB-26a: yield: 5.1%, R.sub.f: 0.32; MS (ESI+): 742.1; maximum absorption wavelength: 614 nm; molar extinction coefficient: 20,500 M.sup.1cm.sup.−1; singlet oxygen yield: 20%. HB-26b: yield: 5.8%, R.sub.f: 0.34; MS (ESI+): 742.1; maximum absorption wavelength: 622 nm; molar extinction coefficient: 20,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 18%. HB-26c: yield: 11.1%, R.sub.f: 0.37; MS (ESI+): 742.1; maximum absorption wavelength: 614 nm; molar extinction coefficient: 21,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 21%. HB-26d: yield: 3.8%. R.sub.f: 0.32; MS (ESI+): 742.1; maximum absorption wavelength: 622 nm; molar extinction coefficient: 20,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 16%. Structural formulas of the above amino-substituted products are as follows:

##STR00108##

Example 68

[0219] Preparation of an aspartic acid-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—CH(COOH)—CH.sub.2COOH, R.sub.3═—COCH.sub.3, R.sub.4═—H): a substituted amino raw material is an aspartic acid, a synthetic method is similar to the preparation of the diaminoacetic acid-substituted hypocrellin B derivative in example 17, and four blue black solid products HB-27a-HB-27d are obtained, respectively. HB-27a: yield: 7.4%, R.sub.f: 0.36; MS (ESI+): 744.1; maximum absorption wavelength: 621 nm; molar extinction coefficient: 20,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 18%. HB-27b: yield: 6.6%, R.sub.f: 0.32; MS (ESI+): 744.1; maximum absorption wavelength: 620 nm; molar extinction coefficient: 20,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 19%. HB-27c: yield: 7.9%, R.sub.f: 0.30; MS (ESI+): 744.1; maximum absorption wavelength: 625 nm; molar extinction coefficient: 21,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 21%. HB-27d: yield: 5.2%, R.sub.f: 0.28; MS (ESI+): 744.1; maximum absorption wavelength: 620 nm; molar extinction coefficient: 21,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 18%. Structural formulas of the above amino-substituted products are as follows:

##STR00109##

Example 69

[0220] Preparation of a di-glutamic acid-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—CH(COOH)—CH.sub.2CH.sub.2COOH, R.sub.3═—COCH.sub.3, R.sub.4═—H): a substituted amino raw material is an glutamic acid, a synthetic method is similar to the preparation of the diaminoacetic acid-substituted hypocrellin B derivative in example 14, and four blue black solid products HB-28a-HB-28d are obtained, respectively. HB-28a: yield: 7.4%, R.sub.f: 0.36; MS (ESI+): 772.1; maximum absorption wavelength: 621 nm; molar extinction coefficient: 20,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 18%. HB-28b: yield: 6.6%, R.sub.f: 0.32; MS (ESI+): 772.1; maximum absorption wavelength: 620 nm: molar extinction coefficient: 21,000 M.sup.4 cm.sup.−1; singlet oxygen yield: 19%. HB-28c: yield: 7.9%, R.sub.f: 0.30; MS (ESI+): 772.1; maximum absorption wavelength: 625 nm; molar extinction coefficient: 21,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 22%. HB-28d: yield: 5.2%, R.sub.f: 0.28; MS (ESI+): 772.1; maximum absorption wavelength: 620 nm; molar extinction coefficient: 20,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 18%. Structural formulas of the above amino-substituted products are as follows:

##STR00110##

Example 70

[0221] Preparation of a diaminosulfonic acid-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—(CH.sub.2).sub.n—SO.sub.3H, R.sub.3═R.sub.4═—H): a substituted amino raw material is NH.sub.2—(CH.sub.2)—SO.sub.3H (m=2, 3, 4, 6), a synthetic route is similar to the preparation of the aminobutyric acid-polyethylene glycol (of different chain lengths)-substituted deacetyl hypocrellin derivative in example 20, and four blue black solid products HC-29a-Cn-N.sup.+, HC-29b-Cn-N.sup.+, HC-29c-Cn-N.sup.+, HC-29d-Cn-N.sup.+ (n=2, 4, 6) are obtained, respectively. HC-29a-C.sub.2—N.sup.+ (n=2): yield: 11.6%. R.sub.f: 0.30: MS (ESI+): 642.3; maximum absorption wavelength: 622 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HC-29b-C.sub.4—N.sup.+ (n=4): yield: 9.8%, R.sub.f: 0.38; MS (ESI+): 698.6; maximum absorption wavelength: 622 nm; molar extinction coefficient: 30,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 36%. HC-29c-C6-N.sup.+ (n=6): yield: 14.8%, R.sub.f: 0.40: MS (ESI+): 754.6; maximum absorption wavelength: 628 nm; molar extinction coefficient: 33,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 39%. HC-29d-C.sub.4—N.sup.+ (n=4): yield: 13.2%, R.sub.f: 0.32: MS (ESI+): 698.6; maximum absorption wavelength: 628 nm: molar extinction coefficient: 32,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 33%. Structural formulas of the above amino-substituted products are as follows:

##STR00111##

Example 71

[0222] Preparation of a 4-tranexamic acid-triphenylphosphine salt (of different chain lengths)-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—CH.sub.2C.sub.6H.sub.10COO—Cn-N(CH.sub.3).sub.3, R.sub.3═R.sub.4═—H) (n is the number of carbon atoms of the quaternary ammonium salt, and n=2, 4, 6): a synthetic route is similar to the preparation of the aminobutyric acid-polyethylene glycol (of different chain lengths)-substituted deacetyl hypocrellin derivative in example 20, and four blue black solid products HC-30a-Cn-PPh.sub.3.sup.+, HC-30b-Cn-PPh.sub.3+, HC-30c-Cn-PPh.sub.3.sup.+, HC-30d-Cn-PPh.sub.3.sup.+ (n=2, 4.6) are obtained, respectively. HC-30a-C.sub.2—PPh.sub.3 (n=2): yield: 10.6%. R.sub.f: 0.30; MS (ESI+): 1328.5; maximum absorption wavelength: 624 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HC-30b-C.sub.4—PPh.sub.3.sup.+ (n=4): yield: 9.5%, R.sub.f: 0.36; MS (ESI+): 1384.5; maximum absorption wavelength: 624 nm; molar extinction coefficient: 30,500M.sup.−1cm.sup.−1: singlet oxygen yield: 36%. HC-30c-C6-PPh.sup.+ (n=6): yield: 17.5%, R.sub.f:0.42, MS (ESI+): 1440.6; maximum absorption wavelength: 632 nm; molar extinction coefficient: 33,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 40%. HC-30d-C.sub.4—PPh.sub.3+(n=4): yield: 11.2%. R.sub.f:0.32; MS (ESI+): 1384.5; maximum absorption wavelength: 630 nm; molar extinction coefficient: 32,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 31%. Structural formulas of the above amino-substituted products are as follows:

##STR00112## ##STR00113##

Example 72

[0223] Preparation of a 4-tranexamic acid-triphenylphosphine salt (of different chain lengths)-substituted hypocrellin derivative (R.sub.1═R.sub.2═—CH.sub.2C.sub.6H.sub.10CO—NH—Cn-N(CH.sub.3).sub.3, R.sub.3═—COCH.sub.3, R.sub.4═—H) (n is the number of carbon atoms of the quaternary ammonium salt, and n=2, 4, 6): a synthetic route is similar to the preparation of the aminobutyric acid-polyethylene glycol (of different chain lengths)-substituted deacetyl hypocrellin derivative in example 20, and four blue black solid products HB-30a-NH—Cn-PPh.sub.3.sup.+, HB-30b-NH—Cn-PPh.sub.3.sup.+, HB-30c-NH—Cn-PPh.sub.3.sup.+, HB-30d-NH—Cn-PPh.sub.3.sup.+ (n=2, 4, 6) are obtained, respectively. HB-30a-NH—C.sub.2—PPh.sub.3.sup.+ (n=2): yield: 8.6%. R.sub.f: 0.32; MS (ESI+): 1371.5; maximum absorption wavelength: 625 nm; molar extinction coefficient: 31,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 34%. HB-30b-NH—C.sub.4—PPh.sub.3.sup.+ (n=4): yield: 9.2%, R.sub.f: 0.38: MS (ESI+): 1427.5; maximum absorption wavelength: 625 nm; molar extinction coefficient: 30,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 36%. HB-30c-NH—C.sub.6—PPh.sub.3.sup.+ (n=6): yield: 18.5%. R.sub.f: 0.40; MS (ESI+): 1483.6; maximum absorption wavelength: 630 nm; molar extinction coefficient: 33,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 38%. HB-30d-NH—C.sub.4—PPh.sub.3.sup.+ (n=4): yield: 13.2%, R.sub.f: 0.30: MS (ESI+): 1427.5; maximum absorption wavelength: 632 nm; molar extinction coefficient: 32,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 34%. Structural formulas of the above amino-substituted products are as follows:

##STR00114## ##STR00115##

Example 73

[0224] Preparation of a 4-aminomethylpiperidine-PEG (of different chain lengths)-substituted hypocrellin derivative (R.sub.1═R.sub.2═—CH.sub.2C.sub.5H.sub.9N—CO-PEGn, R.sub.3═—COCH.sub.3, R.sub.4═—H) (PEG is polyethylene glycol, and n=1, 6, 12): a synthetic route is similar to the preparation of the aminobutyric acid-polyethylene glycol (of different chain lengths)-substituted deacetyl hypocrellin derivative in example 20, and four blue black solid products HB-31a-PEGn, HB-31b-PEGn, HB-31c-PEGn, HB-31d-PEGn (n=1, 6, 12) are obtained, respectively. HB-31a-PEG1 (n=1): yield: 7.6%, R.sub.f: 0.30; MS (ESI+): 966.5; maximum absorption wavelength: 622 nm; molar extinction coefficient: 32.000 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HB-31b-PEG6 (n=6): yield: 8.2%, R.sub.f: 0.35; MS (ESI+): 1406.5; maximum absorption wavelength: 622 nm; molar extinction coefficient: 31,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 38%. HB-31c-PEG6 (n=6): yield: 19.5%, R.sub.f: 0.41: MS (ESI+): 1406.6; maximum absorption wavelength: 632 nm: molar extinction coefficient: 33,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 40%. HB-31d-PEG12 (n=12): yield: 13.9%, R.sub.f: 0.30; MS (ESI+): 1934.5; maximum absorption wavelength: 632 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 33%. Structural formulas of the above amino-substituted products are as follows:

##STR00116## ##STR00117##

Example 74

[0225] Preparation of a 4-aminomethylpiperidine-PEG (of different chain lengths)-substituted bromo-hypocrellin derivative (R.sub.1═R.sub.2═—CH.sub.2C.sub.5HN—CO-PEGn, R.sub.3═—COCH.sub.3, R.sub.4═—Br) (PEG is polyethylene glycol, and n=1, 6, 12): a synthetic route is similar to the preparation of the aminobutyric acid-polyethylene glycol (of different chain lengths)-substituted deacetyl hypocrellin derivative in example 20, and four blue black solid products HB-31a-Br-PEGn, HB-31b-Br-PEGn, HB-31c-Br-PEGn, HB-31d-Br-PEGn (n=1, 6, 12) are obtained, respectively. HB-31a-Br-PEG1 (n=1): yield: 5.6%, R.sub.f: 0.35; MS (ESI+): 1045.5; maximum absorption wavelength: 624 nm: molar extinction coefficient: 32,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 33%. HB-31b-Br-PEG6 (n=6): yield: 8.5%, R.sub.f: 0.30: MS (ESI+): 1485.5; maximum absorption wavelength: 624 nm; molar extinction coefficient: 31.500 M.sup.−1cm.sup.−1; singlet oxygen yield: 31%. HB-31c-Br-PEG6 (n=6): yield: 16.5%, R.sub.f: 0.40; MS (ESI+): 1485.6; maximum absorption wavelength: 631 nm; molar extinction coefficient: 33,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 38%. HB-31d-Br-PEG12 (n=12): yield: 10.9%, R.sub.f: 0.32: MS (ESI+): 2013.5; maximum absorption wavelength: 632 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 33%. Structural formulas of the above amino-substituted products are as follows:

##STR00118## ##STR00119##

Example 75

[0226] Preparation of a 4-aminomethylpiperidine-PEG (of different chain lengths)-substituted hypocrellin derivative (R.sub.1═R.sub.2═—CH.sub.2C.sub.5H.sub.9N-PEGn, R.sub.3═—COCH.sub.3, R.sub.4═—H) (PEG is polyethylene glycol, and n=1, 6, 12): hypocrellin B HB (100 mg, 0.18 mmol) and 4-aminomethylpiperidine (2 mmol) were dissolved in 100 mL of anhydrous acetonitrile, after fully mixed, a mixture was heated to 80° C. under the protection of nitrogen and stirred in a lucifugous condition for a reaction for 20 h, a solvent was distilled off after the reaction, a blue black solid was dissolved in 100 mL of dichloromethane, a solution was washed with distilled water three times, an organic layer was dried and filtered, and an organic phase was spin-dried to obtain a crude product. The obtained crude product was dissolved in 50 mL of anhydrous dichloromethane, K.sub.2CO.sub.3 (500 mg) was added, to react with bromo-polyethylene glycol (Br-PEGn-OCH.sub.3, 2 g) of different chain lengths, respectively, and a reaction solution was stirred in a lucifugous condition at room temperature for a reaction for 8 h. The reaction solution was added to 100 mL of dichloromethane, a mixed solution was washed once with 100 mL of a dilute hydrochloric acid aqueous solution and then washed with distilled water three times, an organic layer was dried by using anhydrous magnesium sulfate and filtered, and the crude product was separated out by means of thin-layer chromatography, with a developing agent being a mixed solution of ethyl acetate:ethanol=5:1, to obtain blue black solid products HB-32a-PEGn, HB-32b-PEGn, HB-32c-PEGn, HB-32d-PEGn (n=1, 6, 12), respectively. HB-32a-PEG1 (n=1): yield: 7.6%. R.sub.f: 0.30; MS (ESI+): 910.5; maximum absorption wavelength: 622 nm; molar extinction coefficient: 32,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HB-32b-PEG6 (n=6): yield: 8.2%, R.sub.f: 0.35; MS (ESI+): 1350.5; maximum absorption wavelength: 622 nm; molar extinction coefficient: 31,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 38%. HB-32c-PEG6 (n=6): yield: 19.5%, R.sub.f: 0.41; MS (ESI+): 1350.6; maximum absorption wavelength: 632 nm; molar extinction coefficient: 33,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 40%. HB-32d-PEG12 (n=12): yield: 13.9%. R.sub.f: 0.30; MS (ESI+): 1878.5; maximum absorption wavelength: 632 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 33%. Structural formulas of the above amino-substituted products are as follows:

##STR00120## ##STR00121##

Example 76

[0227] Preparation of a di-n-propylamine-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—CH.sub.2CH.sub.2CH.sub.3, R.sub.3═—COCH.sub.3, R.sub.4═—H): a synthetic method is similar to the preparation of the di-2-(2-aminoethoxy)ethanol-substituted hypocrellin B derivative in example 2, and four blue black solid products HB-33a-HB-33d are obtained, respectively. HB-33a: yield: 6.4%, R.sub.f: 0.37; MS (ESI+) 596.2; maximum absorption wavelength: 618 nm; molar extinction coefficient: 32,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 30%. HB-33b: yield: 6.5%, R.sub.f: 0.39; MS (ESI+): 596.2; maximum absorption wavelength: 615 nm; molar extinction coefficient: 33,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HB-33c: yield: 5.6%, R.sub.f: 0.36; MS (ESI+): 596.2; maximum absorption wavelength: 618 nm; molar extinction coefficient: 34,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 35%. HB-33d: yield: 4.9%, R.sub.f: 0.30; MS (ESI+): 596.2; maximum absorption wavelength: 620 nm, molar extinction coefficient: 32,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. Structural formulas of the above amino-substituted products are as follows:

##STR00122##

Example 77

[0228] Preparation of a diaminohexyl-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—C.sub.6H.sub.13, R.sub.3═R.sub.1═—H): a synthetic method is similar to the preparation of the di-2-(2-aminoethoxy)ethanol-substituted hypocrellin B derivative in example 2, and four blue black solid products HC-34a-HC-34d are obtained, respectively. HC-34a: yield: 5.4%, R.sub.f: 0.35; MS (ESI+): 638.6; maximum absorption wavelength: 625 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 30%. HC-34b: yield: 6.2%, R.sub.f: 0.32; MS (ESI+): 638.6; maximum absorption wavelength: 620 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HC-34c: yield: 6.4%, R.sub.f: 0.28; MS (ESI+): 638.6; maximum absorption wavelength: 624 nm; molar extinction coefficient: 32,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 38%. HC-34d: yield: 5.5%, R.sub.f: 0.16; MS (ESI+): 638.6; maximum absorption wavelength: 628 nm: molar extinction coefficient: 32,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. Structural formulas of the above amino-substituted products are as follows:

##STR00123##

Example 78

[0229] Preparation of a hydroxymethyl cyclopropylamine-substituted derivative of dicyclopropylamine-substituted hypocrellin (R.sub.1═R.sub.2═—C.sub.3H.sub.4CH.sub.2OH, R.sub.3═—COCH.sub.3, R.sub.4═—H): a synthetic method is similar to the preparation of the di-2-(2-aminoethoxy)ethanol-substituted hypocrellin B derivative in example 2, and four blue black solid products HB-35a-HB-35d are obtained, respectively. HB-35a: yield: 7.2%, R.sub.f: 0.35; MS (ESI+): 652.2; maximum absorption wavelength: 621 nm; molar extinction coefficient: 30,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HB-35b: yield: 6.7%, R.sub.f: 0.34; MS (ESI+): 652.2; maximum absorption wavelength: 620 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 30%. HB-35c: yield: 6.8%, R.sub.f: 0.28; MS (ESI+): 652.2; maximum absorption wavelength: 619 nm; molar extinction coefficient: 32,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 34%. HB-35d: yield: 4.6%, R.sub.f: 0.27; MS (ESI+): 652.2; maximum absorption wavelength: 621 nm, molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 30%. Structural formulas of the above amino-substituted products are as follows:

##STR00124##

Example 79

[0230] Preparation of a hydroxylmethyl cyclopropylamine-polyethylene glycol (of different chain lengths)-substituted hypocrellin derivative (R.sub.1═R.sub.2═—C.sub.3H.sub.4CH.sub.2O—CO-PEGn, R.sub.3═—COCH.sub.3, R.sub.4═—H) (PEG is polyethylene glycol, and n=1, 6, 12): a synthetic route is similar to the preparation of the aminobutyric acid-polyethylene glycol (of different chain lengths)-substituted deacetyl hypocrellin derivative in example 20, and four blue black solid products HB-35a-PEGn-HB-35d-PEGn are obtained, respectively. HB-35a-PEG1 (n=1): yield: 9.2%, R.sub.f: 0.35: MS (ESI+): 912.2; maximum absorption wavelength: 624 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 34%. HB-35b-PEG6 (n=6): yield: 8.7%, R.sub.f: 0.34; MS (ESI+): 1352.2; maximum absorption wavelength: 624 nm; molar extinction coefficient: 30,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HB-35c-PEG6 (n=6): yield: 12.8%. R.sub.f: 0.32: MS (ESI+): 1352.2; maximum absorption wavelength: 630 nm; molar extinction coefficient: 33,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 38%. HB-35d-PEG12 (n=12): yield: 5.6%, R.sub.f: 0.28: MS (ESI+): 1880.2; maximum absorption wavelength: 625 nm, molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 30%. Structural formulas of the above amino-substituted products are as follows:

##STR00125##

Example 80

[0231] Preparation of a hydroxylmethyl cyclopropylamine-polyethylene glycol (of different chain lengths)-substituted hypocrellin derivative (R.sub.1═R.sub.2═—C.sub.3H.sub.4CH.sub.2—COO-PEGn, R.sub.3═R.sub.4═—H) (PEG is polyethylene glycol, and n=1, 6, 12): a synthetic route is similar to the preparation of the aminobutyric acid-polyethylene glycol (of different chain lengths)-substituted deacetyl hypocrellin derivative in example 20, and four blue black solid products HC-35a-PEGn-HC-35d-PEGn are obtained, respectively. HC-35a-PEG1 (n=1): yield: 7.8%, R.sub.f: 0.28; MS (ESI+): 754.2; maximum absorption wavelength: 622 nm: molar extinction coefficient: 31,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HC-35b-PEG6 (n=6): yield: 8.9%, R.sub.f: 0.35; MS (ESI+): 1194.2; maximum absorption wavelength: 622 nm: molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HC-35c-PEG6 (n=6): yield: 13.8%, R.sub.f: 0.35: MS (ESI+): 1194.2; maximum absorption wavelength: 632 nm: molar extinction coefficient: 33,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 39%. HC-35d-PEG12 (n=12): yield: 4.6%, R.sub.f: 0.30: MS (ESI+): 1622.2; maximum absorption wavelength: 628 nm, molar extinction coefficient: 32,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. Structural formulas of the above amino-substituted products are as follows:

##STR00126##

Example 81

[0232] Preparation of a diaminoethyl-substituted hypocrellin derivative (R.sub.1═R.sub.2═—NHC.sub.2H.sub.5, R.sub.3═—COCH.sub.3, R.sub.4═—H): a substituted amino group is NH.sub.2—NHC.sub.2H, a synthetic method is similar to the preparation of the di-2-(2-aminoethoxy)ethanol-substituted hypocrellin B derivative in example 2, and four blue black solid products HB-36a-HB-36d are obtained, respectively. HB-36a: yield: 6.2%, R.sub.f: 0.38; MS (ESI+): 598.2; maximum absorption wavelength: 621 nm; molar extinction coefficient: 30,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HB-36b: yield: 5.7%, R.sub.f: 0.33; MS (ESI+): 598.2; maximum absorption wavelength: 622 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 30%. HB-36c: yield: 6.5%, R.sub.f: 0.31; MS (ESI+): 598.2; maximum absorption wavelength: 621 nm; molar extinction coefficient: 33,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 35%. HB-36d: yield: 5.6%. R.sub.f: 0.27; MS (ESI+): 598.2; maximum absorption wavelength: 621 nm: molar extinction coefficient: 32,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. Structural formulas of the above amino-substituted products are as follows:

##STR00127##

Example 82

[0233] Preparation of a dihydroxyamino-substituted hypocrellin derivative (R.sub.1═R.sub.2═—NHC.sub.6H.sub.4CH.sub.3, R.sub.3═R.sub.4═—H): a substituted amino raw material is NH.sub.2—OH, a synthetic method is similar to the preparation of the di-2-(2-aminoethoxy)ethanol-substituted hypocrellin B derivative in example 2, and four blue black solid products HC-37a-HC-37d are obtained, respectively. HC-37a: yield: 8.2%, R.sub.f: 0.33; MS (ESI+): 502.5; maximum absorption wavelength: 620 nm; molar extinction coefficient: 30,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HC-37b: yield: 6.7%. R.sub.f: 0.37; MS (ESI+): 502.5; maximum absorption wavelength: 622 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 31%. HC-37c: yield: 6.0%, R.sub.f: 0.31; MS (ESI+): 502.5; maximum absorption wavelength: 624 nm; molar extinction coefficient: 34,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 36%. HC-37d: yield: 6.6%, R.sub.f: 0.27; MS (ESI+): 502.5; maximum absorption wavelength: 620 nm; molar extinction coefficient: 32,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 33%. Structural formulas of the above amino-substituted products are as follows:

##STR00128##

Example 83

[0234] Preparation of a benzylaminopyridine-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—CH.sub.2C.sub.5H.sub.4N, R.sub.3═—COCH.sub.3, R.sub.4═—H): a substituted amino raw material is NH—CH.sub.2CH.sub.4N, a synthetic method is similar to the preparation of the di-2-(2-aminoethoxy)ethanol-substituted hypocrellin B derivative in example 2, and four blue black solid products HB-38a-HB-38d are obtained, respectively. HB-38a: yield: 6.4%, R.sub.f: 0.36; MS (ESI+): 694.6; maximum absorption wavelength: 624 nm; molar extinction coefficient: 19,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 16%; HB-38b: yield: 7.2%, R.sub.f: 0.36; MS (ESI+): 694.6; maximum absorption wavelength: 621 nm; molar extinction coefficient: 18,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 16%; HB-38c: yield: 7.8%. R.sub.f: 0.28; MS (ESI+): 694.6; maximum absorption wavelength: 628 nm; molar extinction coefficient: 20,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 18%; HB-38d: yield: 8.6%, R.sub.f: 0.25; MS (ESI+): 694.6; maximum absorption wavelength: 624 nm; molar extinction coefficient: 18,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 17%. Structural formulas of the above amino-substituted products are as follows:

##STR00129##

Example 84

[0235] Preparation of a di-benzylaminomethylpyridinium-substituted hypocrellin B derivative (R.sub.1═R.sub.2═—CH.sub.2C.sub.1H.sub.4N.sup.+(CH.sub.3), R.sub.3═—COCH.sub.3, R.sub.4═—H): a substituted amino raw material is NH—CH.sub.2CH.sub.4N.sup.+(CH.sub.3), a synthetic method is similar to the preparation of the di-2-(2-aminoethoxy)ethanol-substituted hypocrellin B derivative in example 2, and four blue black solid products HB-38a-N.sup.+-HB-38d-N.sup.+ are obtained, respectively. HB-38a-N.sup.+: yield: 4.4%, R: 0.35; MS (ESI+): 724.8; maximum absorption wavelength: 620 nm; molar extinction coefficient: 22,000M.sup.−1cm.sup.−1; singlet oxygen yield: 18%; HB-38b-N.sup.+: yield: 8.2%, R.sub.f: 0.30; MS (ESI+): 724.8; maximum absorption wavelength: 620 nm; molar extinction coefficient: 21,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 20%; HB-38c-N.sup.+: yield: 4.4%, R.sub.f: 0.20; MS (ESI+): 724.8; maximum absorption wavelength: 622 nm; molar extinction coefficient: 22.500 M.sup.−1cm.sup.−1; singlet oxygen yield: 21%; HB-38d-N.sup.+: yield: 5.6%, R.sub.f: 0.23; MS (ESI+): 724.8; maximum absorption wavelength: 626 nm; molar extinction coefficient: 21,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 20%. Structural formulas of the above amino-substituted products HB-38a-N.sup.+˜HB-38d-N.sup.+ are as follows:

##STR00130##

Example 85

[0236] Preparation of a diamino quaternary ammonium salt-substituted hypocrellin derivative (R.sub.1═R.sub.2═—CH.sub.2C.sub.5H.sub.4N.sup.+(CH.sub.2OH R—COCH.sub.3, R.sub.4═—H): a substituted amino raw material is NH.sub.2—CH.sub.2C.sub.5H.sub.4N.sup.+(CH.sub.2CH.sub.2CH.sub.2COOH), a synthetic method is similar to the preparation of the di-2-(2-aminoethoxy)ethanol-substituted hypocrellin B derivative in example 2, and four blue black solid products HB-38a-N—COOH—HB-38d-N—COOH are obtained, respectively. HB-38a-N—COOH: yield: 3.4%, R.sub.f: 0.55: MS (ESI+): 868.8; maximum absorption wavelength: 620 nm; molar extinction coefficient: 22,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 20%; HB-38b-N—COOH: yield: 6.2%, R.sub.f: 0.50; MS (ESI+): 868.8; maximum absorption wavelength: 620 nm; molar extinction coefficient: 21,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 18%; HB-38c-N—COOH: yield: 10.4%, R.sub.f: 0.51; MS (ESI+): 868.8; maximum absorption wavelength: 622 nm; molar extinction coefficient: 21,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 21%; HB-38d-N—COOH: yield: 5.6%, R.sub.f: 0.53: MS (ESI+): 868.8; maximum absorption wavelength: 626 nm: molar extinction coefficient: 21,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 20%. Structural formulas of the above amino-substituted products HB-38a-N—COOH—HB-38d-N—COOH are as follows:

##STR00131##

Example 86

[0237] Preparation of a dipiperazine-substituted hypocrellin B derivative

##STR00132##

R.sub.3═—COCH.sub.3, R.sub.4═—H): a substituted amino raw material is

##STR00133##

a synthetic route is similar to the preparation of the diaminobutyric acid-substituted polyethylene glycol-modified hypocrellin B derivative in example 17, and four blue black solid products HB-39a-HB-39d are obtained, respectively. HB-39a: yield: 6.4%, R.sub.f: 0.35: MS (ESI+): 878.8; maximum absorption wavelength: 622 nm; molar extinction coefficient: 22,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 20%. HB-39b: yield: 9.2%, R.sub.f: 0.32; MS (ESI+): 878.8; maximum absorption wavelength: 620 nm; molar extinction coefficient: 23,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 21%. HB-39c: yield: 8.4%, R.sub.f:0.26; MS (ESI+): 878.8; maximum absorption wavelength: 628 nm molar extinction coefficient: 21,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 29%. HB-39d; yield: 6.6%, R.sub.f:0.25; MS (ESI+): 878.8; maximum absorption wavelength: 626 nm, molar extinction coefficient; 21,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 22%. Structural formulas of the above amino-substituted products are as follows:

##STR00134##

Example 87

[0238] Preparation of an aminoethyl diketopiperazine-substituted hypocrellin derivative (R.sub.1═R.sub.2═—CH.sub.2CH.sub.2—CO-piperazine, R.sub.3═—COCH.sub.3, R.sub.4═—H): a synthetic method is similar to the preparation of the di-2-(2-aminoethoxy)ethanol-substituted hypocrellin B derivative in example 2, and four blue black solid products HB-40a-HB-40d are obtained, respectively. HB-40a: yield: 4.8%, R.sub.f:0.25; MS (ESI+): 880.2; maximum absorption wavelength: 620 nm; molar extinction coefficient: 31000M.sup.−1cm.sup.−1; singlet oxygen yield: 30%. HB-40b: yield: 5.0%, R.sub.f:0.34; MS (ESI+): 880.2; maximum absorption wavelength: 624 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 34%. HB-40c: yield: 14.5%, R.sub.f: 0.42; MS (ESI+): 880.2; maximum absorption wavelength: 632 nm; molar extinction coefficient: 34,000M.sup.−1cm.sup.−1; singlet oxygen yield: 40%. HB-40d: yield: 6.8%, R.sub.f: 0.30; MS (ESI+): 880.2; maximum absorption wavelength: 628 nm, molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 30%. Structural formulas of the above amino-substituted products are as follows:

##STR00135##

Example 88

[0239] Preparation of a diketopiperazine-polyethyleneglycol-substituted hypocrellin derivative (R.sub.1═R.sub.2═—CH.sub.2CH.sub.2O—CO-piperazine-PEGn, R.sub.3═—COCH.sub.3, R.sub.4═—H) (PEG is polyethylene glycol, and n=1, 6, 12): a synthetic route is similar to the preparation of the aminobutyric acid-polyethylene glycol (of different chain lengths)-substituted deacetyl hypocrellin derivative in example 20, and four blue black solid products HB-40a-PEGn-HB-40d-PEGn are obtained, respectively. HB-40a-PEG1 (n=): yield: 9.8%, R.sub.f: 0.25; MS(ESI+): 1084.2; maximum absorption wavelength: 624 nm molar extinction coefficient: 31.500M.sup.−1cm.sup.−1; singlet oxygen yield: 32. HB-40b-PEG6 (n=6): yield: 5.9%, R.sub.f: 0.30: MS(ESI+): 1524.2; maximum absorption wavelength: 624 nm molar extinction coefficient: 31,000M.sup.−1cm.sup.−1; singlet oxygen yield: 34%. HB-40c-PEG6 (n=6): yield: 12.8%, R.sub.f: 0.38: MS(ESI+): 1524.2 maximum absorption wavelength: 630 nm; molar extinction coefficient: 33,500M.sup.−1cm.sup.−1; singlet oxygen yield: 36%. HB-40d-PEG12 (n=12): yield: 6.1%, R.sub.f:0.30; MS (ESI+): 2052.2; maximum absorption wavelength: 628 nm: molar extinction coefficient: 30,500M.sup.−1cm.sup.−1; singlet oxygen yield: 30%. Structural formulas of the above amino-substituted products are as follows:

##STR00136## ##STR00137##

Example 89

[0240] Preparation of a DABACO-substituted hypocrellin derivative: a synthetic method is similar to the preparation of the di-2-(2-aminoethoxy)ethanol-substituted hypocrellin B derivative in example 2, and four blue black solid products HB-41a-HB-41d are obtained, respectively. HB-41a: yield: 5.4%, R.sub.f: 0.34; MS (ESI+): 820.9; maximum absorption wavelength: 622 nm; molar extinction coefficient: 32,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 31%. HB-41b: yield: 6.8%, R.sub.f: 0.38; MS (ESI+): 820.9; maximum absorption wavelength: 624 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HB-41c: yield: 4.8%, R.sub.f: 0.26; MS (ESI+): 820.9; maximum absorption wavelength: 621 nm; molar extinction coefficient: 33,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 35%. HB-41d: yield: 8.8%, R.sub.f: 0.30; MS (ESI+): 820.9; maximum absorption wavelength: 625 nm; molar extinction coefficient: 30,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 28%. Structural formulas of the above amino-substituted products are as follows:

##STR00138##

Example 90

[0241] Preparation of an aminomorpholine-substituted hypocrellin B derivative

##STR00139##

R.sub.3═—COCH.sub.3, R.sub.4═—H): a synthetic route is similar to the preparation of the aminobutyric acid-polyethylene glycol (of different chain lengths)-substituted deacetyl hypocrellin derivative in example 20, and four blue black solid products HB-42a˜HB-42d are obtained, respectively. HB-42a: yield: 4.4%, R.sub.f:0.35; MS (ESI+): 881.8; maximum absorption wavelength: 620 nm; molar extinction coefficient: 32500M.sup.−1cm.sup.−1; singlet oxygen yield: 33%. HB-42b: yield: 8.2%, R.sub.f: 0.30; MS (ESI+): 881.8; maximum absorption wavelength: 620 nm; molar extinction coefficient: 33,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 28%. HB-42c: yield: 4.4%, R.sub.f: 0.20; MS (ESI+): 881.8; maximum absorption wavelength: 622 nm; molar extinction coefficient: 32,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 36%. HB-42d: yield: 5.6%, R.sub.f: 0.23; MS (ESI+): 881.8; maximum absorption wavelength: 626 nm; molar extinction coefficient: 32,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 30%. Structural formulas of the above amino-substituted products are as follows:

##STR00140##

Example 91

[0242] Preparation of an aminoacetic acid/aminobutyric acid-substituted hypocrellin B derivative (R.sub.1═—CH.sub.2COOH, R.sub.2═—CH.sub.2(CH.sub.2).sub.2COOH R.sub.3═—COCH.sub.3, R.sub.4═—H): hypocrellin B HB (100 mg, 0.18 mmol), aminoacetic acid (10 mmol), and NaOH (2 g) were dissolved in 100 mL of a mixed solution of DMF and water (at a volume ratio of 1:1), and after fully mixed, a mixed solution was heated to 120° C. under the protection of nitrogen and stirred in a lucifugous condition for a reaction for 10h. After the reaction, dilute hydrochloric acid was added to adjust the pH to weak acidity, and filtration was performed to collect a precipitate. An obtained crude product was added to aminobutyric acid (10 mmol), and NaOH (2 g) and dissolved in 100 mL of a mixed solution of DMF and water (at a volume ratio of 1:1), and after full mixed, a mixed solution was heated to 100° C. under the protection of nitrogen and stirred in a lucifugous condition for a reaction for 8h. After the reaction, dilute hydrochloric acid was added to adjust the pH to weak acidity, and filtration was performed to collect a precipitate. An obtained blue black solid was dissolved in 200 mL of dichloromethane, a solution was washed once with 100 mL of a dilute hydrochloric acid aqueous solution and then washed twice with distilled water, an organic layer was dried by using anhydrous magnesium sulfate and filtered, and an organic phase was spin-dried to obtain a crude product. The obtained crude product was separated out by means of thin-layer chromatography, with a developing agent being a mixed solution of ethyl acetate, diethylamine, and ethanol (at a volume ratio of 20:1:2), to obtain four blue black solid products HB-43a-HB-43d, respectively. HB-43a: yield: 7.1%, R.sub.f: 0.34; MS (ESI+): 656.2; maximum absorption wavelength: 613 nm; molar extinction coefficient: 26,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 28%; HB-43b: yield: 7.0%, R.sub.f: 0.39; MS (ESI+): 656.2; maximum absorption wavelength: 618 nm; molar extinction coefficient: 26,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 25%. HB-43c: yield: 6.3%, R.sub.f: 0.32; MS (ESI+): 656.2; maximum absorption wavelength: 626 nm; molar extinction coefficient: 26,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 30%. HB-43d: yield: 4.7%, R.sub.f: 0.30; MS (ESI+): 656.2; maximum absorption wavelength: 623 nm; molar extinction coefficient: 26,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 24%. Structural formulas of the above amino-substituted products are as follows:

##STR00141##

Example 92

[0243] Preparation of an ethamine/cyclopentylamine-substituted hypocrellin B derivative (R.sub.1═—C.sub.2H, R.sub.2═—C.sub.5H.sub.9, R.sub.3═—COCH.sub.3, R.sub.4═—H): substituted amino raw materials are NH.sub.2—C.sub.2H.sub.5 and NH.sub.2—C.sub.5H.sub.9, and a synthetic method is similar to the preparation of the aminoacetic acid/aminobutyric acid-modified hypocrellin B in example 83. A developing agent for thin-layer chromatography separation is a mixed solution of ethyl acetate:ethanol=25:1, and four blue black solid products HB-44a-HB-44d are obtained, respectively. HB-44a: yield: 8.0%, R.sub.f: 0.38 MS (ESI+): 608.2; maximum absorption wavelength: 628 nm; molar extinction coefficient: 21,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 20%; HB-44b: yield: 6.0%, R.sub.f: 0.36; MS (ESI+): 608.2; maximum absorption wavelength: 621 nm; molar extinction coefficient: 21,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 18%; HB-44c: yield: 5.9%, R.sub.f: 0.33; MS (ESI+): 608.2; maximum absorption wavelength: 624 nm; molar extinction coefficient: 21,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 20%; HB-44d: yield: 5.1%. R.sub.f: 0.29; MS (ESI+): 608.2; maximum absorption wavelength: 622 nm; molar extinction coefficient: 21,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 21%. Structural formulas of the above amino-substituted products are as follows:

##STR00142##

Example 93

[0244] Preparation of an ethamine/cyclopentylamine-substituted hypocrellin derivative (R.sub.1═—C.sub.2H, R.sub.2═—C.sub.5H.sub.9. R.sub.3═—COCH.sub.3. R.sub.4═—SCH.sub.2CH.sub.2OH): substituted amino raw materials are NH.sub.2—C.sub.2H.sub.5 and NH.sub.2—C.sub.5H.sub.9, and a synthetic method is similar to the preparation of the aminoacetic acid/aminobutyric acid-modified hypocrellin B in example 83. A developing agent for thin-layer chromatography separation is a mixed solution of ethyl acetate:ethanol=25:1, and four blue black solid products HB-S-44a-HB-S-44d are obtained, respectively. HB-S-44a: yield: 8.7%, R.sub.f: 0.32; MS (ESI+): 684.2; maximum absorption wavelength: 628 nm; molar extinction coefficient: 21,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 20%; HB-S-44b: yield: 6.5%. R.sub.f: 0.35; MS (ESI+): 684.2; maximum absorption wavelength: 622 nm; molar extinction coefficient: 21,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 18%; HB-S-44c: yield: 10.9%, R.sub.f: 0.39; MS (ESI+): 684.2; maximum absorption wavelength: 628 nm; molar extinction coefficient: 21,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 20%; HB-S-44d: yield: 4.1%. R.sub.f: 0.29; MS (ESI+): 684.2; maximum absorption wavelength: 622 nm; molar extinction coefficient: 21,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 21%. Structural formulas of the above amino-substituted products are as follows:

##STR00143##

Example 94

[0245] Preparation of an ethanesulfonic acid/propanesulfonic acid-substituted deacetyl hypocrellin derivative (R.sub.1═—CH.sub.2SO.sub.3H. R.sub.2═—CH.sub.2(CH.sub.2).sub.2SO.sub.3H, R.sub.3═R.sub.4═—H): substituted amino raw materials are NH.sub.2—CH.sub.2SO.sub.3H and NH.sub.2—CH.sub.2(CH.sub.2).sub.2SO.sub.3H, and a synthetic method is similar to the preparation of the aminoacetic acid/aminobutyric acid-modified hypocrellin B in example 83. A developing agent for thin-layer chromatography separation is a mixed solution of ethyl acetate:ethanol=8:1, and four blue black solid products HC-45a-HC-45d are obtained, respectively. HC-45a: yield: 7.0%, R.sub.f: 0.30; MS (ESI+): 686.9; maximum absorption wavelength: 613 nm; molar extinction coefficient: 21,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 18%; HC-45b: yield: 7.7%, R.sub.f: 0.36; MS (ESI+): 686.9; maximum absorption wavelength: 618 nm; molar extinction coefficient: 21,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 20%; HC-45c: yield: 5.3%, R.sub.f: 0.32; MS (ESI+): 686.9; maximum absorption wavelength: 627 nm; molar extinction coefficient: 22,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 21%; HC-45d: yield: 4.8%, R.sub.f: 0.39; MS (ESI+): 686.9; maximum absorption wavelength: 624 nm; molar extinction coefficient: 21,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 21%. Structural formulas of the above amino-substituted products are as follows:

##STR00144##

Example 95

[0246] Preparation of an ethylhydrazine/aspartic acid-substituted hypocrellin B derivative (R.sub.1═—NHC.sub.2H, R.sub.2═—CH(COOH)—CH.sub.2COOH, R.sub.3═R.sub.4═—H): substituted amino raw materials are NH.sub.2—NHC.sub.2H.sub.5 and NH.sub.2—CH(COOH)—CH.sub.2COOH, a synthetic method is similar to the preparation of the aminoacetic acid/aminobutyric acid-modified hypocrellin B in example 83, and four blue black solid products HC-46a-HC-46d are obtained, respectively. HC-46a: yield: 5.0%, R.sub.f: 0.39 MS (ESI+): 671.2; maximum absorption wavelength: 629 nm: molar extinction coefficient: 22,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 21%; HC-46b: yield: 6.5%, R.sub.f: 0.35; MS (ESI+): 671.2; maximum absorption wavelength: 620 nm; molar extinction coefficient: 21,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 18%: HC-46c: yield: 6.9%, R.sub.f: 0.33; MS (ESI+): 671.2; maximum absorption wavelength: 625 nm; molar extinction coefficient: 20,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 21%; HC-46d: yield: 4.1%, R.sub.f: 0.27; MS (ESI+): 671.2; maximum absorption wavelength: 623 nm; molar extinction coefficient: 22,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 23%. Structural formulas of the above amino-substituted products are as follows:

##STR00145##

Example 96

[0247] Preparation of an aminobutyric acid/amino polyethylene glycol-substituted hypocrellin derivative (R.sub.1═—CH.sub.2CH.sub.2—PEGn-OCH.sub.3, R.sub.2═—CH.sub.2(CH.sub.2).sub.2COOH, R.sub.3═—COCH.sub.3, R.sub.4═—H) (PEG is polyethylene glycol, n is the number of ethylene glycol units, and n=1, 612): substituted amino raw materials are aminobutyric acid and NH.sub.2—CH.sub.2CH.sub.2—PEGn-OCH.sub.3, a synthetic method is similar to the preparation of the amino acetic acid/amino butyric acid-modified hypocrellin B in example 83, and four blue black solid products HB-47a-PEGn, HB-47b-PEGn, HB-47c-PEGn, HB-47d-PEGn (n=1, 6, 12) are obtained, respectively. HB-47a-PEG1 (n1): yield: 7.4%, R.sub.f: 0.25; MS(ESI+): 700.5; maximum absorption wavelength: 622 nm; molar extinction coefficient: 30,500M.sup.−1cm.sup.−1; singlet oxygen yield: 30%. HB-47b-PEG6 (n=6): yield: 0.30; MS (ESI+): 1140.5; maximum absorption wavelength: 624 nm; molar extinction coefficient: 30,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 30%. HB-47c-PEG12 (n=12): yield: 18.2%, R.sub.f: 0.35; MS (ESI+): 1678.5; maximum absorption wavelength: 630 nm; molar extinction coefficient: 33,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 38%. HB-47d-PEG6 (n=6): yield: 7.9%, R.sub.f: 0.32; MS (ESI+): 1140.5; maximum absorption wavelength: 628 nm; molar extinction coefficient: 32,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. Structural formulas of the above amino-substituted products are as follows:

##STR00146##

Example 97

[0248] Preparation of an aminobutyric acid/(2-aminoethoxy)ethanol-polyethylene glycol-substituted hypocrellin derivative (R.sub.1═—CH.sub.2(CH.sub.2).sub.2COOH, R.sub.1═—CH.sub.2CH.sub.2—O—CH.sub.2CH.sub.2—O—CO-PEG.sub.n-OCH.sub.3, R.sub.3═—COCH.sub.3, R.sub.4═—H) (PEG is polyethylene glycol, n is the number of ethylene glycol units, and n=1, 6, 12): substituted amino raw materials are aminobutyric acid and NH.sub.2—CH.sub.2CH.sub.2—O—CH.sub.2CH.sub.2—O—CO-PEG-OCH.sub.3, a synthetic method is similar to the preparation of the aminoacetic acid/aminobutyric acid-modified hypocrellin B in example 83, and four blue black solid products HB-48a-PEGn, HB-48b-PEGn, HB-48c-PEGn, HB-48d-PEGn (n=1, 6, 12) are obtained, respectively. HB-48a-PEG1 (n=1): yield: 8.4%, R.sub.f: 0.28; MS (ESI+): 816.5; maximum absorption wavelength: 628 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HB-48b-PEG6 (n=6): yield: 9.4%, R.sub.f: 0.34; MS (ESI+): 1256.5; maximum absorption wavelength: 628 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HB-48c-PEG12 (n=12): yield: 20.2%, R.sub.f: 0.45; MS (ESI+): 1784.5; maximum absorption wavelength: 636 nm; molar extinction coefficient: 34,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 40%. HB-48d-PEG6 (n=6): yield: 8.5%, Rr: 0.35; MS (ESI+): 1256.5; maximum absorption wavelength: 632 nm; molar extinction coefficient: 32,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. Structural formulas of the above amino-substituted products are as follows:

##STR00147##

Example 98

[0249] Preparation of an aminobutyric acid/4-tranexamic acid-polyethylene glycol-substituted hypocrellin derivative (R.sub.1═—CH.sub.2(CH.sub.2).sub.2COOH, R.sub.2═—CH.sub.2C(H.sub.10COO-PEGn, R.sub.3═R.sub.4—H) (PEG is polyethylene glycol, n is the number of ethylene glycol units, and n=1, 6, 12): a synthetic method is similar to the preparation of the aminoacetic acid/aminobutyric acid-modified hypocrellin B in example 83, and four blue black solid products HC-49a-PEGn, HC-49b-PEGn, HC-49c-PEGn, HC-49d-PEGn (n=1, 6, 12) are obtained, respectively. HC-49a-PEG1 (n=1): yield: 8.8%, R.sub.f: 0.30; MS (ESI+): 765.5; maximum absorption wavelength: 630 nm; molar extinction coefficient: 31,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HC-49b-PEG6 (n=6): yield: 9.8%, R.sub.f: 0.32; MS (ESI+): 1205.5; maximum absorption wavelength: 630 nm; molar extinction coefficient: 31,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 34%. HC-49c-PEG12 (n=12): yield: 17.2%, R.sub.f: 0.40; MS (ESI+): 1733.5; maximum absorption wavelength: 638 nm; molar extinction coefficient: 33,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 38%. HC-49d-PEG6 (n=6): yield: 8.9%, R.sub.f: 0.32; MS (ESI+): 1205.5; maximum absorption wavelength: 632 nm; molar extinction coefficient: 31,500 M.sup.−1cm.sup.−1; singlet oxygen yield: 34%. Structural formulas of the above amino-substituted products are as follows:

##STR00148##

Example 99

[0250] Preparation of an aminoacetic acid/4-aminomethylcyclohexanoic acid-polyethylene glycol-substituted hypocrellin B derivative (R.sub.1═—CH.sub.2COOH, R.sub.2═—CH.sub.2C.sub.6H.sub.10COO-PEGn, R.sub.3═R.sub.4═—H) (PEG is polyethylene glycol, n is the number of ethylene glycol units, and n=1, 6, 12): a synthetic method is similar to the preparation of the aminoacetic acid/aminobutyric acid-modified hypocrellin Bin example 83, and four blue black solid products HC-49a-PEGn, HC-49b-PEGn, HC-49c-PEGn, HC-49d-PEGn (n=1, 6, 12) are obtained, respectively. HC-49a-PEG1 (n=1): yield: 8.8%, R.sub.f: 0.30; MS (ESI+): 765.5; maximum absorption wavelength: 630 nm molar extinction coefficient: 31,000M.sup.−1cm.sup.−1; singlet oxygen yield: 32%. HC-49b-PEG6 (n=6): yield: 9.8%, R: 0.32; MS (ESI+): 1205.5; maximum absorption wavelength: 630 nm; molar extinction coefficient: 31,000M.sup.−1cm.sup.−1; singlet oxygen yield: 34%. HC-49c-PEG12 (n=12): yield: 17.2%, R.sub.f: 0.40; MS(ESI+): 1733.5; maximum absorption wavelength: 638 nm molar extinction coefficient: 33,000 M.sup.−1cm.sup.−1; singlet oxygen yield: 38%. HC-49d-PEG6 (n=6): yield: 8.9%, R: 0.32; MS (ESI+): 1205.5; maximum absorption Structural formulas of the above amino-substituted products are as follows:

##STR00149##

Example 100

[0251] Dark cytotoxicity experiment: cultured Hela cells were digested with 0.25% trypsin and pipetted to prepare a single-cell suspension, the number of cells was adjusted to about 2×10.sup.4 per milliliter, and 200 uL of the suspension was seeded in a well of a 96-well culture plate, which is placed in a 37° C. incubator containing 5% CO.sub.2 for cultivation. After the cells adhere to the wall, a supernatant culture solution was discarded, and photosensitizers of different concentrations, that is, the hematoporphyrin derivative HpD, the hypocrellin B HB, or the compound HB-1c-PEG6 synthesized in example 3, were added in a strictly lucifugous condition in accordance with experimental designs, and the culture plate was placed in the 37° C. incubator containing 5% CO.sub.2 for further cultivation and incubation for 1 hour. A cellular survival rate was measured by means of the MTT method, 20 uL of MTT was added to each well, wherein the MTT is MTT prepared with PBS and having a concentration of 5 mg/ml. The culture plate was placed in the 37° C. incubator containing 5% CO.sub.2 for further cultivation for 4 hours, after which the cultivation was terminated. A supernatant in the well was extracted and discarded carefully. Then, 150 uL of dimethyl sulfoxide was added to each well, and the culture plate was shaken by using a micro shaker for 10 minutes to fully dissolve a purple crystal substance. A wavelength of 570 nm was selected to measure an optical density value (i.e., OD value) of each well on a microplate reader, and the cellular survival rate was calculated in accordance with the following formula: cellular survival rate=OD value of an experimental group/OD value of a blank group×100%. FIG. 12(a) shows a diagram of dark toxicities. As shown in FIG. 12(a), the cytotoxicity (dark toxicity) research test indicates that HB-c-PEG6 synthesized in example 3 has a smaller cytotoxicity, which is similar to that of the hypocrellin B HB and commercial photosensitive drug hematoporphyrin HpD. After the Hela cells are incubated by using the photosensitizer HB-1c-PEG6 with the concentration of 10 μmol/L for a half hour, death of many Hela cells is not observed, indicating that such the photosensitizer is basically non-cytotoxic.

Example 101

[0252] Photo-cytotoxicity experiment on cell: cultured Hela cells were digested and pipetted with 0.25% trypsin and to prepare a single-cell suspension, the number of cells was adjusted to about 2×10.sup.4 per milliliter, and 200 uL of the suspension was seeded in a well of a 96-well culture plate, which is placed in a 37° C. incubator containing 5% CO.sub.2 for cultivation. After the cells adhere to the wall, a supernatant culture solution was discarded, and photosensitizers of different concentrations, that is, the hematoporphyrin derivative HpD, the hypocrellin B HB, or HB-1c-PEG6 synthesized in example 3, were added in a strictly lucifugous condition in accordance with experimental designs, and the culture plate was placed in the 37° C. incubator containing 5% CO.sub.2 for further cultivation and incubation for 1 hour. Then, a semiconductor laser with a wavelength of 635 nm was used for irradiation, a power density was adjusted to 20 mW/cm.sup.2, light beams were evenly and vertically applied onto the 96-well culture plate, and an irradiation time was 1000 S. In addition, each 96-well culture plate is provided with a blank group, and 6 holes were provided for in each condition. After irradiation, the culture plate was placed in the 37° C. incubator containing 5% CO.sub.2 for further cultivation and incubation for 24 hours, and then a cellular survival rate was measured. The cellular survival rate was measured by means of the MTT method, 20 uL of MTT was added to each well, wherein the MTT is MTT prepared with PBS and having a concentration of 5 mg/ml. The culture plate was placed in the 37° C. incubator containing 5% CO.sub.2 for further cultivation for 4 hours, after which the cultivation was terminated. A supernatant in the well was extracted and discarded carefully. Then, 150 uL of dimethyl sulfoxide (DMSO) was added to each well, and the culture plate was shaken by using a micro shaker for 10 minutes to fully dissolve a purple crystal substance. A wavelength of 570 nm was selected to measure an optical density value (i.e., OD value) of each well on a microplate reader, and the cellular survival rate was calculated in accordance with the following formula: cellular survival rate=OD value of an experimental group/OD value of a blank group×100%. FIG. 12(b) shows a diagram of phototoxicities. The cell phototoxicity experiment as shown in FIG. 12(b) indicates that HB-1c-PEG6 presents very strong lethality to the Hela cells under irradiation of red light. HB-1c-PEG6 with a concentration range of 160 nM can kill more than 90% of the Hela cells, while in the same condition, the hypocrellin B or commercial photosensitizer hematoporphyrin derivative can kill only about 20% of the Hela cells, indicating that a photodynamic effect of such the hypocrellin derivative having a peri-position substituted by an amino group is significantly better than that of the hypocrellin B HB and commercial photosensitizer hematoporphyrin HpD.

Example 102

[0253] Dark cytotoxicity experiment on cell: the experimental method and procedure are the same as those in example 100, except that HB-1c-PEG6 synthesized in example 3 is replaced by the polyethylene glycol-di-2-(2-aminoethoxy)ethanol-substituted deacetyl hypocrellin derivative HC-c-PEG6 synthesized in example 6. A result is as shown in FIG. 13(a), and the dark cytotoxicity experiment for the diamino-substituted hypocrellin derivative HC-c-PEG6 synthesized in example 6 has a similar result with example 100.

Example 103

[0254] Photo-cytotoxicity experiment on cell: the experimental method and procedure are the same as those in example 101, except that HB-1c-PEG6 synthesized in example 3 is replaced by the diaminobutyric acid-substituted deacetyl hypocrellin derivative HC-8c synthesized in example 22. A result is as shown in FIG. 14(b), and the photo-cytotoxicity experiment for HC-8c synthesized in example 22 has a similar result with example 101.

Example 104

[0255] Photo-cytotoxicity experiment on cell: the experimental method and procedure are the same as those in example 101, except that HB-1c-PEG6 synthesized in example 3 is replaced by the polyethylene glycol-diaminobutyric acid-modified deacetyl hypocrellin derivative HC-8c-PEG6 synthesized in example 23. A result is as shown in FIGS. 15(a) and 15(b), and the photo-cytotoxicity experiment for HC-8c-PEG6 synthesized in example 23 has a similar result with example 101.

[0256] Obviously, the above embodiments of the present invention are merely examples for clearly explaining the present invention, and are not intended to limit the implementations of the present invention. For those of ordinary skill in the art, based on the above description, they can also make other changes or modifications in different forms. All of the implementations cannot be exhausted herein, and any obvious changes or modifications derived from the technical solutions of the present invention still fall within the protection scope of the present invention.

[0257] In addition, it should be noted that the hypocrellin derivatives involved in this patent that require the protection all have two enol tautomers, and the chemical structures of the two isomers certainly fall within the protection scope. For simplicity, only one of the enol tautomers is listed in all of the examples of this patent, and the other enol tautomer and the corresponding general structural formula thereof are described in detail in the specification, the structure thereof certainly falls within the protection scope.