Systematic device for abyssal sediment pressure-holding transfer

Abstract

A sediment pressure-holding transfer device developed based on abyssal sediment fidelity sampler is disclosed, which uses a vacuum pump to discharge a gas in the device, uses a high-pressure pump as a power source to perform a pressurization process, uses gravity of a sample to perform a first transfer, and uses a high-pressure impurity pump and a safety valve to perform a second transfer of a sample mixture, in its structure, a high-pressure-resistant and corrosion-resistant material is used as a main material of the device, the device mainly comprises a mechanical system and a hydraulic system, the mechanical system is used as a main frame of the device and is a basis for ensuring operation of the device; the hydraulic system is used as a core of the device and is a key to ensure success of sediment transfer.

Claims

1. A sediment pressure-holding transfer device, comprising:—a sediment fidelity sampler; wherein the sediment pressure-holding transfer device uses a vacuum pump to discharge a gas in the device, uses a high-pressure pump as a power source to perform a pressurization process, uses gravity of a sample to perform a first transfer, and uses a high-pressure impurity pump and a safety valve to perform a second transfer of a sample mixture, in its structure, a high-pressure-resistant and corrosion-resistant material is used as a main material of the device, the device comprises a mechanical system and a hydraulic system, the mechanical system is used as a main frame of the device and is a basis for ensuring operation of the device; the hydraulic system is used as a core of the device; the mechanical system and the hydraulic system cooperate together to ensure that a sediment sample is smoothly transferred from a sediment sampling cylinder to a culture kettle, the sediment fidelity sampler has a volume of ≥150 ml and a height of ≥100 mm, and is connected with the pressure-holding transfer device by using a ball valve thread interface; the gas to be discharged refers to air present in a container, and a purpose is to ensure that the sample is not contaminated by other substances in the air during a transfer process, and another purpose is to avoid a danger caused by a poor compression ratio of the gas during the pressurization process; and the pressurization process refers to a continuous filling of a high-pressure liquid into a corresponding container through a high-pressure pump so that a pressure of the container equals to an in-situ pressure of a seabed sample.

2. The sediment pressure-holding transfer device according to claim 1, wherein a pressurized medium used for the pressurization process is a microorganism-specific culture liquid.

3. The sediment pressure-holding transfer device according to claim 1, wherein the main material of the device is Tc4.

4. The sediment pressure-holding transfer device according to claim 1, wherein the transfer process comprises a first transfer and a second transfer, the first transfer refers to a process in which the sediment is transferred from a sampling bucket to the high-pressure magnetic stirring container, the second transfer refers to a process in which a sediment-culture liquid mixture is transferred to the culture kettle.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) FIG. 1 is a general schematic diagram of a sediment pressure-holding transfer system.

(2) FIG. 2 is a schematic diagram of a first transfer system.

(3) FIG. 3 shows a bottom end of the magnetic stirring container.

(4) In the drawings: 1—culture liquid tank, 2—magnet steel, 3—belt, 4—bracket, 5—end cap, 6—cooling water port, 7—ball valve, 8—butt-joint sleeve, 9—pressure cylinder port ball valve, 10—accumulator, 11—push rod, 12—pressure cylinder, 13—needle valve, 14—magnetic stirring container, 15—connector, 16—motor, 17—culture ball valve, 18—gas cylinder, 19—vacuum pump, 20—gas control valve, 21—culture kettle, 22—high-pressure impurity pump, 23—pressure gauge, 24—high-pressure water pump, 25—check valve, 26—propeller, 27—sampling cylinder, 28—piston, 29—inlet, 30—bolt, 31—sensor inlet, 32—outlet

DETAILED DESCRIPTION OF EMBODIMENTS

(5) The present disclosure will be further described in detail below with reference to the accompanying drawings and embodiments.

(6) FIG. 1 shows the overall schematic diagram of the sediment pressure-holding transfer system, which mainly comprises a vacuum device, a booster power device, a sampling device, a butt-joint device, a stirring device, and a culture device.

(7) The vacuum device mainly includes a gas control valve 20, a vacuum pump 19, and a gas cylinder 18. The vacuum device is mainly used to discharge the air inside the whole system before the start of test, and to charge the nitrogen with less activity. This cycle is repeated several times to completely discharge the air. The vacuum device is connected to the culture kettle 21 through a gas passage, and the switches between the culture kettle and the other devices are all opened to form a passage for discharging the air inside the device.

(8) The booster power device mainly includes a culture liquid tank 1, a high-pressure water pump 24, a check valve 25, a pressure gauge 23, and pipes. The booster device mainly pressurizes and delivers the culture liquid in the culture liquid tank into the magnetic stirring container and the culture kettle, the water inlet of the high-pressure water pump 24 is connected to the culture liquid tank, and the other end is connected to the magnetic stirring container and the culture kettle through a three-way interface.

(9) The sampling device mainly comprises a pressure cylinder port ball valve 9, an accumulator 10, a push rod 11, a pressure-holding cylinder 12, a sampling cylinder 27, and a piston 28. The device is used for taking the sediment of the seabed with fidelity. The sediment is stored in the sampling cylinder, and is sealed by the petal structure. The push rod 11 can drive the piston 28 to move, thereby moving the sampling cylinder 27 to realize the auxiliary functions of sampling and transferring. The sampling device is connected to the ball valve 7 via a butt joint sleeve 8.

(10) The butt joint device is specially designed for the butt joint sleeve 8 which is adapted to the system. The bottom section has internal thread and is connected with the external thread of the ball valve 7. The middle section has external thread and is connected with the internal thread of the pressure-holding cylinder port ball valve 9. The annular sheet of the upper end is used to open the petal structure of the sampling cylinder.

(11) The stirring device comprises a stirring container and a magnetic stirrer, and is the intermediate core part of the system. The device is the end of the first transfer, the start of the second transfer, and also technical support for the sample changing from solid to liquid mixture. The magnetic stirrer is rotated through rotation of the magnetic steel 2 by the external motor 16 through the belt 3, thereby stirring the rotor 26 of the rotor portion, and uniformly mixing the sediment sample with the culture liquid. A cooling water circulation passage is left in the periphery of the container to maintain the temperature inside the container. A sensor inlet 31 and a water port 32 are left on the end cap.

(12) The culture device is the final destination of the sediment sample. The culture kettle mainly comprises a kettle body 21 and a kettle lid 17. When the sample is successfully transferred to the culture kettle 21, the culture kettle port ball valve 17 is closed to complete the transfer. The culture kettle 21 is a replaceable device, and after one transfer is completed, another empty culture kettle is exchanged until the entire transfer of the sample is completed.

(13) FIG. 2 shows the schematic diagram of the first transfer system, which is also the core process of this transfer. Temperature compensation and pressure compensation are controlled in this part. At the same time, the whole transfer process is carried out under invisible conditions, so the sectional view is shown.

(14) The working steps of this embodiment will be described below with reference to the accompanying drawings:

(15) 1) Performing butt joint of the sampling device and the pressure-holding transfer device through the ball valve 7;

(16) 2) Opening the vacuum pump 19, discharging the air inside the device to make it in a vacuum state, closing the vacuum pump 19, and closing the pipe connected thereto;

(17) 3) Turning on the high-pressure water pump 24, pressurizing the magnetic stirring container 14 to 100 MPa, pressurizing the culture kettle 21 to 99.2 MPa, after the pressurization is completed, turning off the high-pressure water pump 24, and turning off the switch on the boosting passage;

(18) 4) Opening the connection ball valve 7 between the sampling device and the pressure-holding transfer system, and pushing the piston 28 downward by the structure of the push rod 11 of the sampling device until the petal structure of the sampling barrel is opened by the ring structure built in the pressure-holding transfer device, thereby the sample of the sediment in the sampling tank flowing out into the magnetic stirring container 14 by gravity;

(19) 5) Starting the motor 16, driving the magnetic stirrer 2 through the belt 3 by the motor 16, and evenly stirring the culture liquid and the sediment sample;

(20) 6) Turning on the high-pressure impurity pump 22, extracting the mixture sample out by the pressure difference between the inlet and the outlet of the high-pressure impurity pump, sending the mixture sample to the culture kettle 21, and when the pressure in the culture kettle 21 reaches 100 MPa and the transfer is finished, turning off the high-pressure impurity pump 22 and the culture kettle port ball valve 17;

(21) 7) Removing the culture kettle 21 already sealed, deploying another culture kettle, repeating the above processes, and packing the sample into another culture kettle for study.