Abstract
A device for preparing sample material is designed as a rotary device by means of which a defined quantity of liquid can be drawn in into a sample receiving chamber for the sample material or can be expelled from the sample receiving chamber for the sample material, by means of a rotary motion.
Claims
1. A rotary device for preparing sample material comprising: a sample-receiving space for the sample material, wherein the rotary device is configured to perform a rotational movement to draw in a defined quantity of liquid into the sample-receiving space or expel the defined quantity of liquid from the sample-receiving space.
2. The rotary device as claimed in claim 1, further comprising: a liquid-receiving space; and a rotary body coupled to the liquid-receiving space by a thread, the liquid-receiving space having a volume with a size that is altered by rotation of the rotary body relative to the thread or by rotation of the thread relative to the rotary body.
3. The rotary device as claimed in claim 2, further comprising: a hollow body defining the liquid-receiving space and that is equipped internally with the thread.
4. The rotary device as claimed in claim 3, wherein the hollow body has a sealed push-through region at an end directed away from the sample-receiving space.
5. The rotary device as claimed in claim 4, wherein the sealed push-through region has a needle carrying the sample material.
6. The rotary device as claimed in claim 2, wherein the liquid-receiving space is fluidically connected to the sample-receiving space.
7. The rotary device as claimed in claim 2, further comprising: a tube body defining the sample-receiving space, the tube body being open at its an directed away from the liquid-receiving space.
8. The rotary device as claimed in claim 7, wherein one of the tube body and the rotary body has an outer thread portion which complements an inner thread portion arranged in the liquid-receiving space.
9. The rotary device as claimed in claim 7, wherein at least one of the tube body, a hollow body that defines the liquid-receiving space, and the rotary body is combined with at least one sealing device.
10. The rotary device as claimed in claim 7, wherein at least one of the tube body, a hollow body that defines the liquid-receiving space, and the rotary body is combined with a filter device.
11. The rotary device as claimed in claim 2, wherein one of the liquid-receiving space and the sample-receiving space has an attachment configured for delivery and/or discharge of a fluid.
12. The rotary device as claimed in claim 11, wherein the attachment is configured as a third attachment on a T-piece, which delimits the liquid-receiving space and/or the sample-receiving space.
13. The rotary device as claimed in claim 12, wherein the T-piece has a first attachment for the rotary device, a second attachment for the sample-receiving space, and the third attachment for delivery and/or discharge of the fluid.
14. A microfluidic system comprising: a rotary device for preparing sample material, the device comprising: a sample-receiving space for the sample material, wherein the rotary device is configured to perform a rotational movement to draw in a defined quantity of liquid into the sample-receiving space or expel the defined quantity of liquid from the sample-receiving space.
15. A method for preparing sample material comprising: using a rotary device, which has a sample-receiving space for receiving the sample material, to perform a rotational movement to draw in a defined quantity of liquid into the sample receiving space or expel the defined quantity of liquid from the sample receiving space.
Description
BRIEF DESCRIPTION OF THE DRAWING
[0039] FIG. 1 shows a schematic view, in longitudinal section, of a device for preparing sample material, with a rotary body arranged rotatably in a hollow body;
[0040] FIG. 2 shows a schematic view, in longitudinal section, of a tube body which, with the aid of a sealing device, is connected in a fluid-tight manner to a coupling body;
[0041] FIG. 3 shows a schematic view, in longitudinal section, of a tube body which is combined with an attachment body;
[0042] FIG. 4 shows, in longitudinal section, a similar device to that of FIG. 1, with a sealing device and with two abutments;
[0043] FIG. 5 shows, in longitudinal section, a similar device to that of FIG. 4, with a sample removal device;
[0044] FIG. 6 shows a similar device to that of FIG. 5, with a symbolically indicated PCR bead on the sample removal device;
[0045] FIG. 7 shows, in longitudinal section, a similar device to that of FIG. 5, with an additional third abutment;
[0046] FIG. 8 shows, in longitudinal section, the device from FIG. 7 after the additional abutment has been passed;
[0047] FIG. 9 shows a flow diagram illustrating a method for preparing sample material using a device as shown for example in FIGS. 1 and 4 to 8;
[0048] FIG. 10 shows, in section, a microfluidic system with a lab-on-a-chip and with an open end of a tube body of the device from FIG. 1;
[0049] FIG. 11 shows, in longitudinal section, the open end of the tube body of the device from FIG. 1 with sample material, with a microfluidic container and with a magnet device;
[0050] FIG. 12 shows, in longitudinal section, the device from FIG. 5 with an additional filter device at the open end of the tube body;
[0051] FIG. 13 shows, in longitudinal section, a similar device to that of FIG. 1, with a sample removal device and with a sliding seal;
[0052] FIG. 14 shows a further illustrative embodiment of a device for preparing sample material, with an additional T-piece;
[0053] FIGS. 15 to 17 show a schematic view of a method in which the device from FIG. 14 is used to transport fluid completely out of a cannula;
[0054] FIG. 18 shows a variant of the device from FIG. 14, when a syringe is attached to the T-piece;
[0055] FIG. 19 shows a similar view to that of FIG. 18, when a syringe is attached to the T-piece from above;
[0056] FIGS. 20 to 23 show a schematic illustration of a method by which the device from FIG. 14 can advantageously be integrated, in a fluidic outlet for a lysis process with cells adhering to a biopsy needle, into a lab-on-a-chip platform; and
[0057] FIGS. 24 to 26 show a use of the device from FIG. 18, wherein two phases are stored in the syringe.
DESCRIPTION OF THE ILLUSTRATIVE EMBODIMENTS
[0058] FIG. 1 shows schematically, in longitudinal section, a device 1 configured as a rotary device for preparing sample material 23, 33 (in FIGS. 2, 3, 5 to 8, 12, 13).
[0059] The rotary device 1 comprises a rotary body 2 which is rotatable in a hollow body 4 with the aid of a thread 3. The hollow body 4 has the shape of a straight circular cylinder, which is closed at its upper end in FIG. 1. The hollow body 4 delimits a liquid-receiving space 5.
[0060] The thread 3 comprises an inner thread portion 6 in the hollow body 4. An outer thread portion 7 of the thread 3 engages in the inner thread portion 6. The outer thread portion 7 is formed on a collar 8 of the rotary body 2. The collar 8 is angled away from a main body 9 of the rotary body 2.
[0061] The main body 9 of the rotary body 2 comprises a central through-hole, which leads into a tube body 10. The tube body 10 is configured, for example, as a capillary 11 with an open end 12 at the bottom. The capillary 11 delimits on the inside a sample-receiving space 15, which is fluidically connected to the liquid-receiving space 5 in the hollow body 4 via the central through-hole in the rotary body 2.
[0062] A double arrow 13 in FIG. 1 indicates that the tube body 10 moves up and down with the rotary body 2 when the rotary body 2 is rotated relative to the hollow body 4. The movement of the rotary body 2, indicated by the double arrow 13, is also designated as a stroke. The stroke of the rotary body 2 changes the volume of the liquid-receiving space 5, as is indicated by a double arrow 14 in FIG. 1.
[0063] When the volume of the liquid-receiving space 5 becomes smaller, liquid is expelled from the liquid-receiving space 5 through the sample-receiving space 15 and the open end 12 of the tube body 10. When the volume of the liquid-receiving space 5 becomes greater, liquid is drawn into the liquid-receiving space 5 through the open end 12 of the tube body 10. The liquid is made available by way of a suitable container (not shown in FIG. 1) at the open end 12 of the tube body 10.
[0064] FIG. 2 shows how a tube body 20, which is configured as a capillary 21, can be attached to a rotary body (2 in FIG. 1). To provide a fluid-tight connection, a seal 22 is mounted on the capillary 21. Above the seal 22, an end portion of the capillary 21 is arranged inside a coupling body 26. The coupling body 26 comprises a tip 27 which is configured similarly to the tip of a pipet. The free end of the tip 27 of the coupling body 26 bears sealingly on the seal 22.
[0065] The coupling body 26 is connected, for example, to a rotary body, as shown in FIG. 1 and labeled by 2. The connection between the rotary body and the coupling body 26 can be an integral connection. Sample material 23 on a sample removal device 24 is arranged in the capillary 21. The sample removal device 24 is configured as a biopsy needle 25. In FIG. 2, only a functional or functionalized portion of the biopsy needle 25 is arranged in the capillary 21.
[0066] FIG. 3 shows a tube body 30 configured as a cannula 31. Sample material 33 on a sample removal device 34 is arranged in the cannula 31. The sample removal device 34 comprises, for example, a biopsy needle 35, of which the functional portion is arranged inside the cannula 31. The rest of the biopsy needle 35 is guided through an attachment body 39 with a coupling device 37.
[0067] The attachment body 39 is connected integrally to the tube body 30. On its right-hand side in FIG. 3, the attachment body 39 has an attachment 40, for example for a rotary device (not shown in FIG. 3). The attachment 40 is combined with a filter device 32.
[0068] The coupling device 37 comprises a Luer lock element 38, which comprises a push-through region for the biopsy needle 35. A free end 36 protrudes upward in FIG. 3 out of the coupling device 37.
[0069] The attachment body 39 tapers to a point toward the tube body 30. The attachment body 39, with the tube body 30 and the coupling device 37 and the filter device 32, is configured for example as a disposable part. The rotary device that is attached to the attachment 40 is then configured, for example, as a reusable part. The filter 32 in this case advantageously serves to reduce a danger of contamination.
[0070] FIG. 4 shows how a predefined volume can be drawn in with a rotary device 41. For this purpose, the rotary device 41 has to be airtight and, during the intake of liquid, must be rotated by a precisely defined number of revolutions. The rotary device 41 comprises a rotary body 42, which is coupled to a hollow body 44 via a thread 43. A sealing device 45 is arranged for sealing between the rotary body 42 and the hollow body 44.
[0071] The thread 43 is provided in the hollow body 44 with two abutments 46, 47, by which the rotation of the rotary body 42 in the hollow body 44 is limited. The rotary body 42 comprises a main body 49 with a central through-hole. In FIG. 4, a collar 48 is angled away from the top of the main body 49. At its lower end in FIG. 4, the rotary body 42 is connected to a tube body 50.
[0072] The tube body 50 comprises a sample-receiving space 15, which is connected to a liquid-receiving space 5 inside the rotary body 42 or inside the hollow body 44. As a result of the increasing space inside the rotary device 41, a negative pressure is obtained, which ensures that a precisely defined volume of liquid is sucked through the open end of the tube body 50 into the rotary device 41.
[0073] FIGS. 5 and 6 show a device 51 configured as a rotary device and having a rotary body 52 which is rotatable in a hollow body 54 via a thread 53. A sealing device 55 serves for sealing between the rotary body 52 and the hollow body 54 with the thread 53. The rotary body 52 comprises a collar 58, which is angled away from a main body 59. The main body 59 is connected to a tube body 60, which is configured as a capillary or cannula.
[0074] A sample removal device 34, which is configured as a biopsy needle 35, is arranged in the tube body 60. The sample material 33 is arranged on the sample removal device 34 and is prepared for analysis with the aid of liquid. The liquid is sucked into the liquid-receiving space 5 through the sample-receiving space 15 inside the tube body 50. For this purpose, the rotary body 52 is rotated in a defined manner. The rotation of the rotary body 52 relative to the hollow body 54 is limited by two abutments 56, 57 on the thread 53.
[0075] The rotary body 52 can also be designated as an adapter piece and is configured in FIG. 5 as a Luer lock with a rubber septum. This affords the advantage that a rigid probe, for example the biopsy needle 35, or a functionalized wire is already equipped with a Luer lock. Laborious working of the wire is thus avoided, which greatly minimizes the danger of undesired contamination or destruction of the probe. Moreover, the probe does not first of all have to be laboriously processed and can instead be used and treated directly.
[0076] FIG. 6 shows the possibility of a combination of several steps for subsequently carrying out a quantitative real-time polymerase chain reaction (PCR) within the device 51. A PCR bead 61 is, for example, provided inside the rotary body 52. Chemicals required for the preparation, for example lyophilizate, can be stored for example in dry form.
[0077] FIGS. 7 and 8 show a device 71 which is configured as a rotary device and with which a multi-step method can be carried out. The device 71 comprises a rotary body 72 which is rotatable in a hollow body 74 with the aid of a thread 73. Two sealing devices 75, 76 are provided for sealing between the hollow body 74 and the rotary body 72.
[0078] The rotary body 72 comprises a collar 78, which is angled away from a main body 79 of the rotary body 72. The main body 79 of the rotary body 72 merges into a tube body 80 which, as in the preceding illustrative embodiments, comprises the sample removal device 34.
[0079] The hollow body 74 is combined with a sealing cylinder 77. The sealing cylinder 77 has the form of a straight circular cylinder and is closed at its upper end in FIG. 7. A non-functional portion of the sample removal device extends through the closed end of the sealing cylinder 77.
[0080] The rotary body 72 comprises a central recess 70 in which the lower end of the sealing cylinder 77 in FIG. 7 engages. The sealing cylinder 77 is rigidly connected to the hollow body 74. The rotary body 72 is rotatable relative to the sealing cylinder 77. The thread 73 and the sealing devices 75, 76 are arranged in an annular space which is delimited radially to the inside by the sealing cylinder 77 and radially to the outside by the hollow body 74. The collar 78 of the rotary body 72 is rotatable via the thread 73 between a total of three abutments 66, 67, 68.
[0081] A lower abutment is designated by 66. An upper abutment is designated by 67. An additional central abutment 68 is arranged between the two abutments 66 and 67. In FIG. 7, the collar 78 of the rotary body 72 is arranged between the two abutments 66 and 68. With the different abutments 66 to 68, it is possible in a simple way to define different volumes which are expelled or drawn in by the device 71 during the rotation of the rotary body 72 relative to the hollow body 74.
[0082] When a multi-step method is carried out with the device 71 in FIG. 7, a first volume V1 is taken up once or several times for example in a first step. For this purpose, the rotary device 71 with the collar 78 is rotated from the lower abutment 66 as far as the additional central abutment 68. In a further step, a second volume V2 is metered by means of the device being rotated as far as the upper abutment 67. The second volume is greater than the first volume.
[0083] FIG. 8 shows how the rotary body 72 with the collar 78 is moved between the two abutments 66 and 67 in order to draw in or expel the second volume. The additional central abutment (68 in FIG. 7) is destroyed in FIG. 8 after a first pass and is therefore no longer present.
[0084] In FIG. 9, rectangles 81 to 84 illustrate a method in which the device 1; 41; 51; 71; 131 is used and by which a sample removal device 24; 34 equipped for example with cells, in particular a wire equipped with cells, is prepared for a genetic analysis. Here, the cells are intended to be lyzed in the device, and the lysate is to be transferred into a microfluidic analysis unit. The rectangle 81 indicates a washing step. The probe needle is washed, for example, with a phosphate-buffered saline solution in order to remove any possible residues from the sample, for example blood, fat, culture medium. For this purpose, the washing liquid is drawn into the device through the open end of the tube body and expelled again.
[0085] The rectangle 82 indicates a fixing step. In the fixing step, the sample is biologically fixed such that no further biochemical reactions take place in the cells.
[0086] For this purpose, a fixing solution, for example formaldehyde or acetone, is taken up by the device, incubated and expelled again. This is advantageously followed by a further brief washing step.
[0087] The rectangle 83 indicates a lysis step. In the lysis step, internal cell material, such as proteins or nucleic acids, is released by the lysis. For this purpose, a lysis solution, for example distilled water, is taken up and the cells incubated therein.
[0088] The rectangle 84 indicates a sample transfer step. Here, the lysate from step 83 is transferred directly into a microfluidic analysis unit. The microfluidic analysis unit belongs to a microfluidic system, as is designated by 100 in FIG. 10.
[0089] The microfluidic system 100 in FIG. 10 comprises a lab-on-a-chip 101. The lab-on-a-chip 101 comprises a microfluidic channel system 102. For the sample transfer, the tube body 10 is arranged with its opening 12 in an insertion opening 103 of the lab-on-a-chip 101. The positioning of the tube body 10 is made easier by an abutment 104 in the insertion opening 103.
[0090] With the aid of the rotary device, a defined quantity of fluid, in particular liquid, for preparing a sample can then be easily drawn in from the lab-on-a-chip 101. After the sample has been prepared, it can then be expelled, likewise with the aid of the rotary device, into a corresponding sample chamber of the lab-on-a-chip 101.
[0091] FIG. 11 shows a use in a microfluidic system 110 with possible magnetic cleaning. The microfluidic system 110 comprises a microfluidic container 111 with magnetic beads 112. In the magnetic cleaning, the magnetic beads 112 are introduced with a functionalized surface into the tube body 10 and expelled again. Depending on the nature of the functionalization, the beads bind either disruptive constituent parts or the desired sample. For this purpose, the tube body 10, configured for example as a cannula, is advantageously enclosed by a magnetic device 113. The magnetic beads 112 are transported in the tube body 10 by a corresponding movement of the magnetic device 113.
[0092] Using the example of the device 71 illustrated in FIG. 7, FIG. 12 shows that a filter device 120 can also be placed at the open end 12 of the tube body 80. Purification or pre-purification of the sample can be easily carried out with the filter device 120.
[0093] FIG. 13 shows a device 131 similar to the device 1 from FIG. 1. The same reference signs as in FIG. 1 are used to designate the same or similar parts. To avoid repetition, reference is made to the above description of FIG. 1.
[0094] A sample removal device 34 with sample material 33 is arranged in the device 131. In contrast to the device 1 in FIG. 1, the device 131 comprises a sliding seal 132 for the sealing between the rotary body 2 and the hollow body 4. The sliding seal 132 permits relatively simple sealing and is advantageously secured on the hollow body 4.
[0095] In FIGS. 14 to 26, a device 141 for preparing sample material with a rotary device 142 is shown schematically in different configurations and uses. At its upper end in the figures, the device 141 is closed in a fluid-tight and pressure-tight manner by a septum 143. The septum 143 is, for example, a stopper made of an elastic material through which a biopsy needle 144 is pushed.
[0096] The biopsy needle 144 extends through the septum 143 into the interior of the device 141. A portion 146 of the biopsy needle 144 is arranged in a functional region 145 of the device 141. The portion 146 of the biopsy needle 144 is preferably a functionalized portion.
[0097] The rotary device 142 comprises a rotary body 147 which, as has been described above, is movable in an axial direction, i.e. downward and upward in FIGS. 14 to 26, via a thread (not shown in FIGS. 14 to 26), when the rotary body 147 is rotated.
[0098] The functional region 145 of the device 141 is configured as a sample-receiving body 148. The sample-receiving body 148 can also be designated as a tube body and is configured, for example, as a capillary.
[0099] Between the sample-receiving body 148 and the rotary device 142, the device 141 comprises a T-piece 150. The T-piece 150 has a first attachment 151 for the sample-receiving body 148, and a second attachment 152 for the rotary device 142. As is indicated in FIG. 14 by a broken line, the biopsy needle 144 extends lengthwise from the top downward through the septum 143, through the rotary body 147 and through the T-piece 150 into the functional region 145 of the device 141.
[0100] The T-piece 150 comprises a third attachment 153 which, in FIG. 14, is closed in a fluid-tight and pressure-tight manner by a closure body 155. In FIGS. 14 to 18, 21, 22 and 24 to 26, the third attachment 153 of the T-piece 150 is arranged perpendicularly or transversely with respect to the longitudinal extent of the device 141.
[0101] FIG. 19 shows the device 141 with a T-piece 170 in which attachments 171 and 172 correspond to the attachments 151 and 152 of the T-piece 150. In contrast to the T-piece 150, a third attachment 173 of the T-piece 170 is arranged parallel to the longitudinal extent or longitudinal axis of the device 141.
[0102] The sample-receiving body 148 in FIG. 14 is, for example, a cannula in which the functionalized biopsy needle 144 with sample material is arranged for fluidic processing. For this purpose, the sample-receiving body 148 is attached via the T-piece 150 to the rotary device 142 with which, by a rotational movement of the rotary body 147, fluid can be drawn in through the open end of the cannula 148 and expelled.
[0103] The third attachment 153 of the T-piece 150 makes available a channel which, during the operation of the device 141, can be used to convey liquids from above through the T-piece 150 into the cannula 148. In this way, a flow or stream through the cannula 148 can be easily generated.
[0104] The closure body 155 is configured, for example, as a rotary closure cap and is preferably standardized for Luer parts. If so required, the closure body 155 can be unscrewed in order to introduce a fluid, with a suitable device such as a syringe, into the device 141 through the third attachment 153 of the T-piece 150.
[0105] FIGS. 15 to 17 show how the device 141 from FIG. 14 is used to completely remove a fluid, in particular a liquid 154 with the sample, from the cannula 148. For this purpose, as is indicated in FIG. 17, an inert phase 159, in particular an oil phase, is transported through the cannula 148 in order to collect the fluid, in particular the sample material 154, in a container 156 at the open end of the cannula 148. The container 156 preferably belongs to a lab-on-a-chip.
[0106] The method shown in FIGS. 15 to 17 is particularly of importance in connection with a biopsy needle when the material adhering to the biopsy needle, generally cells, is to be transferred in the smallest possible volume and the entire volume is to be further processed. Lysis in a small volume is important particularly in the enrichment of rare cells, for example circulating tumor cells with defined mutations, immune cells with defined epitopes and antigens or stem cells.
[0107] In addition, the lysate should be able to be further processed without losses. With the device 141, fluid can be taken up into the cannula 148 with the aid of the rotary device 142, as is seen in FIG. 15. Possible lysate residues, for example individual droplets, may remain in the cannula 148, as is indicated in FIG. 16. As can be seen in FIG. 17, these lysate residues are displaced from the cannula 148 by the follow-on movement of oil, until the entire lysate is collected in the container.
[0108] The follow-on movement of the oil phase takes place via a fluid delivery device 157, as is indicated in FIG. 17 by an arrow 158. A slow follow-on movement of the oil phase prevents undesired mixing in the two-phase system. Thus, the oil can then be decanted off by a phase separation or can be further used as a seal.
[0109] If a defined quantity of lysis buffer is stored in the container 156, it is then also possible for only a proportion of this volume to be drawn in for lysis and, as described above, returned completely into the storage vessel or the container 156. Volume retention is thus permitted, as a result of which it is possible to dispense with complicated volume adjustment and complicated volume measurements.
[0110] FIGS. 18 and 19 show that the oil phase can be introduced into the device 141 via the attachment 153; 173 with the aid of a syringe 162. The syringe 162 is advantageously configured as a Luer part and can be screwed instead of the closure body (155 in FIG. 14) onto the third attachment 153; 173 of the T-piece 150; 170. Thus, the syringe 162 can be used as a fluid delivery device. In order to apply the oil phase, a piston of the syringe 162 can be actuated. In FIG. 18, the syringe 162 is arranged orthogonally with respect to the biopsy needle. In FIG. 19, the syringe 162 is arranged parallel to the biopsy needle on account of the different configuration of the T-piece 170.
[0111] FIGS. 20 to 23 show a method by which the device 141, advantageously in a fluidic outlet for a lysis process with cells adhering to a biopsy needle, can be integrated into a lab-on-a-chip platform. In the schematically indicated process, a liquid phase 183 is stored in a sample input chamber 182. The sample input chamber 182 is provided, for example, in a lab-on-a-chip 181 of a microfluidic system 180.
[0112] The liquid phase or liquid 183 is a lysis buffer which is present in the volume, in order later to provide a lyophilized bead with the chemicals for a subsequent analysis reaction, for example sequencing. The above-described device 141 with the biopsy needle (not shown in FIGS. 20 to 23) is then used to draw in as much lysis buffer 183 as is needed to fill the cannula completely, as can be seen in FIG. 21. The lysis can also be performed by repeated raising and lowering of lysis buffer. The stroke in the raising and lowering of the lysis is initiated by rotation of the rotary body 147, as is indicated in FIG. 21 by an arrow 184.
[0113] Then, as is indicated in FIG. 22 by an arrow 187, the lysate is expelled completely from the device 141 by oil 188. As a result of phase separation, the now preserved lysis buffer volume, including cell material, is located in the sample input chamber 182, which is also designated as storage chamber. The lysis buffer volume is then additionally overlaid by the oil.
[0114] As is indicated in FIG. 23 by arrows 191 and 192, the sample input chamber 182 be controlled by suitable microfluidics of the microfluidic system 180. Here, a feed channel 194 of the sample input chamber 182 is filled by means of oil 193. The lysate is then enclosed between two oil phases and can be transported, without loss, in the lab-on-a-chip system 181.
[0115] FIGS. 24 to 26 show the use with a syringe 162 in which a first phase 201 and a second phase 202 are stored. The first phase 201 is an aqueous phase, for example. The second phase 202 is an oil phase, for example. A phase separation is advantageously achieved by a parallel arrangement of the syringe 162, as is shown in FIG. 19. The desired phase separation has the effect that the two phases 201, 202 do not mix in the syringe 162.
[0116] A double arrow 204 in FIG. 24 indicates that the device 141 can be used with the rotary device 142 in order to draw fluid in and to expel fluid.
[0117] An arrow 211 in FIG. 25 indicates that the for example aqueous phase 201 is first of all pushed through the cannula 148 and can be pumped up and down with the aid of the syringe 162, as is indicated by arrows 212 to 214 in FIG. 25. The lysis buffer and the lysate thus mix with the liquid 201. This is particularly of interest when using a lysis method that consists of several steps. Thus, for example, basic lysis buffers are neutralized by an acid buffer before the lysate is further processed.
[0118] An arrow 218 in FIG. 26 indicates that, at the end of the method, the second phase or oil phase 202 is introduced from the syringe 162 into the device 141.
