GAS SUPPLY DEVICE

Abstract

A gas supply device for operation in an incubator allowing treatment of a culture within a shaker flask with gas independently of the composition and humidity of the gas atmosphere within the incubator individually supplies each shaker flask of the incubator with gas using a gas supply clamp detachably connected to the closure cap of each flask. The gas supply clamp has at least two elastic clamping jaws and a web connecting the clamping jaws to one another. The clamping jaws exert a clamping force on the side wall of the closure cap. The clamp is held in place by the elastic clamping jaws. Above a sterile filter in the closure cap, an individual gas atmosphere for treatment of the culture in the shaker flask is produced in a recess of the web above a gas passage in the closure cap.

Claims

1.-16. (canceled)

17. A gas supply device for a shaker flask, comprising: a hollow-cylindrical closure cap having a top, a lower opening, and a side wall extending between the top and the lower opening, wherein the top has a gas passage therethrough; a sterile filter arranged on and sealed against an inside of the top underneath the gas passage, a gas supply clamp connected detachably to the closure cap, the gas supply clamp having two elastic clamping jaws and a web connecting the clamping jaws to one another, the clamping jaws exerting a clamping force on the side wall of the closure cap, the web having a recess arranged above the gas passage, a seal for the recess against an outside of the top, an inlet for introduction of gas into the recess, and an outlet for exit of gas from the recess.

18. The gas supply device as claimed in claim 17, wherein the closure cap is configured as screw closure.

19. The gas supply device as claimed in claim 17, further comprising a holder accommodating the sterile filter arranged on the inside of the top of the closure cap.

20. The gas supply device as claimed in claim 19, wherein the holder has a circumferential periphery, the holder is configured to surround the gas passage, and the sterile filter is accommodated flush against the circumferential periphery.

21. The gas supply device as claimed in claim 17, wherein the two clamping jaws of the gas supply clamp are diametrically opposed clamping jaws.

22. The gas supply device as claimed in claim 17, wherein a curvature of the clamping jaws is matched to a curvature of the side wall of the closure cap.

23. The gas supply device as claimed in claim 17, wherein each of the clamping jaws has a latching device which at least partially overlies a periphery of the lower opening of the closure cap.

24. The gas supply device as claimed in claim 17, wherein the recess completely overlaps the gas passage.

25. The gas supply device as claimed in claim 17, wherein a planar/flat periphery completely surrounds the recess.

26. The gas supply device as claimed in claim 17, wherein an O-ring surrounding the recess is arranged between the top and the web as the seal for the recess against the outside of the top.

27. The gas supply device as claimed in claim 17, wherein the recess and the gas passage are aligned with a longitudinal central axis of the closure cap.

28. The gas supply device as claimed in claim 17, wherein the inlet and the outlet are arranged diametrically to a longitudinal central axis of the closure cap.

29. The gas supply device as claimed in claim 17, further comprising a gas mixing station and a conduit connecting the gas mixing station to the inlet.

30. The gas supply device as claimed in claim 29, further comprising a wash bottle inserted in the conduit between the gas mixing station and the inlet.

31. The gas supply device as claimed in claim 17, wherein the inlet and/or the outlet is closable by a shut-off valve.

32. A shaking incubator having an interior space in which a plurality of shaker flasks having a gas supply device as claimed in claim 17 are arranged.

Description

[0021] The following explains the invention in more detail using the figures.

[0022] The figures show

[0023] FIG. 1A a plan view of a first working example of a gas supply device according to the invention,

[0024] FIG. 1B a section along the line A-A in FIG. 1a,

[0025] FIG. 1C a section along the line B-B in FIG. 1a,

[0026] FIG. 1D a section along the line C-C in FIG. 1a,

[0027] FIG. 2A a plan view of a second working example of the gas supply device of the invention,

[0028] FIG. 2B a section along the line B-B in FIG. 2a,

[0029] FIG. 2C a section along the line C-C in FIG. 2a, and

[0030] FIG. 3 a schematic depiction of a shaking incubator with a plurality of shaker flasks which are equipped with gas supply devices according to the invention.

[0031] The gas supply device for a shaker flask (1) comprises a hollow-cylindrical closure cap (2) which is configured as screw closure and has a top (2.1), a lower opening (2.2) opposite the top (2.1) and also a side wall (2.3) extending between the top (2.1) and the lower opening (2.2). A gas passage (2.4) which can be seen in FIGS. 1B and 1C is arranged in the top (2.1) concentrically with the longitudinal axis (2.5) of the closure cap (2). The gas passage (2.4) has radial and ring-like stiffening elements which are produced in one piece with the closure cap (2) as injection-molded part.

[0032] On the inside of the closure cap (2) a holder (2.6) which surrounds the gas passage (2.4) in a ring-like manner is injection-molded onto the inside of the closure cap (2) and accommodates a sterile filter (3) in a flush manner. The molded-on holder (2.6) at the same time seals the sterile filter from the inside of the top (2.1) of the closure cap (2).

[0033] A gas supply clamp (4) is detachably connected to the closure cap (2). FIGS. 1 and 2 in each case show a closure cap (2) which is correctly connected to the gas supply clamp (4). The gas supply clamp (2) has two diametrically opposite, elastic clamping jaws (4.1, 4.2) and a web (4.3) which connects the two clamping jaws to one another, with the clamping jaws (4.1, 4.2) exerting a clamping force (F.sub.K) on the side wall (2.3) of the closure cap (2).

[0034] The web (4.3) has an inside facing the outside of the top (2.1) of the closure cap (2). On the inside of the web (4.3), there is a recess (4.4) above the gas passage (2.4). As can be seen in particular, from FIG. 1C in combination with FIG. 1B, the recess (4.4) completely covers the gas passage (2.4). A smooth periphery (4.5) completely surrounds the recess (4.4), with the smooth periphery (4.5) running in a straight line in the longitudinal direction of the web (4.3), as can be seen from FIG. 1B, and in a curved manner in the transition region to the clamping jaws (4.1, 4.2). The smooth periphery (4.5) lies flush against the flat outside of the top (2.1) of the closure cap (2) and thus seals the recess (4.4) against the closure cap (2).

[0035] Two cylindrical passages open into the recess (4.4) of the gas supply clamp (4), with one of the two passages forming an inlet (4.6) for introduction of gas into the recess (4.4) and the other passage forming an outlet for exit of gas from the recess (4.4).

[0036] As can be seen, in particular, from the plan view in FIG. 1A, the curvature of the clamping jaws (4.1, 4.2) is matched to the curvature of the side wall (2.3), which has a circular cross section, of the closure cap (2). The two clamping jaws (4.1, 4.2) are chamfered outward at their lower, free ends. In the region of the chamfer, each clamping jaw (4.1, 4.2) has a latching nose (4.8) which reaches behind the periphery of the lower opening (2.2) of the closure cap (2).

[0037] The gas supply device shown in FIG. 2 has a structure corresponding to that of the gas supply device of FIG. 1. Corresponding components are therefore provided with the same reference numerals. However, there are differences in respect of the shape of the recess (4.4) and the way in which it is sealed against the outside of the top (2.1) of the closure cap (2). The recess (4.4) has a circular cross section which is completely surrounded by a smooth periphery (4.5) which surrounds the recess (4.4) in a ring-like manner. In the smooth periphery, a ring-like circumferential groove (4.9) is let into the web (4.3). The groove (4.9) accommodates an O-ring (4.10) which seals the recess (4.4) against the outside of the top (2.1).

[0038] In order to make the distance from the passages forming the inlet (4.6) and the outlet (4.7) to the longitudinal central axis (2.5) of the closure cap (2) as great as possible, the passages open into the side walls of the recess (4.4) which widen conically from the base of the recess.

[0039] FIG. 3 shows a shaking incubator (5) having an interior space (5.1) in which four shaker flasks (1), which are equipped with a gas supply device according to the invention comprising a closure cap (2) and a gas supply clamp (4), are fixed on a shaker (5.2). The inlets (4.6) of the four shaker flasks (1) are connected in a gas-conducting manner via tubing (5.3), which is shown purely schematically, to a gas distributor and gas scrubber (5.4). The gas distributor and gas scrubber (5.4) is connected via a gas feed conduit (5.5) to a gas mixing station (5.6) which mixes the gas from a plurality of gas sources (5.7) for various gases or gas mixtures, for example air, nitrogen, oxygen and carbon dioxide, according to the gas atmosphere required for the treatment with gas. Such gas mixing stations (5.6) are known per se and are supplied, for example, by the company Adolf Kühner AG, Dinkelbergstrasse 1, CH-4127 Birsfelden (Basle) under the designation FlowCon 2/3/4 (cf. Kuhner shaker, FlowCon 2/3/4 Stand-alone gas mixing device, https://www.kuhner.com/de/produkte/anwendungstechnologien/gas-mixing/flowcon-234.html, downloaded on Nov. 30, 2018).

[0040] The gas mixing station (5.6) and the gas sources (5.7) are arranged outside the shaking incubator (5); only the gas feed conduit (5.5) penetrates through the wall of the shaking incubator (5). The gas or the gas mixture goes via the gas feed conduit (5.5) to the gas distributor and gas scrubber (5.4). This distributes the gas, independently of the gas atmosphere in the interior space (5.1) of the shaking incubator (5), via the conduits (5.3) to the gas supply devices of the individual shaker flasks (1).

[0041] In the gas space above the sterile filter (3) of the gas supply device of each shaker flask (1), a microclimate which has the introduced gas composition and the humidity set by means of the gas distributor and gas scrubber (5.4) is established. Cultivation of the culture media (1.1) in the shaker flasks (1) occurs under the action of this microclimate. If a shaker flask (1) is taken out from the shaking incubator (5), this removal has virtually no influence on the parallel cultures. The slight decrease in the temperature in the interior space (5.1) caused by opening of the incubator door has virtually no effect on the cultivation of the culture media (1.1) in the remaining shaker flasks (1). Due to the gas supply clamp (4) which is particularly easy to handle and can be detached from the closure caps (2), each shaker flask can be provided in a simple way with a gas supply device according to the invention.

[0042] If the gas atmosphere in the shaker flask (1) is to be maintained for the culture after detachment of the gas supply device of the invention from the conduit (5.3), both the inlet and the outlet (4.6, 4.7) can, in an embodiment of the invention which is not shown, in each case be provided with a shut-off valve.

[0043] For this purpose, manually actuatable shut-off valves or pinch valves on tubings, which are connected to the inlet or outlet, can be provided.

TABLE-US-00001 List of reference numerals No. Name 1 Shaker flask 1.1 Culture media 2 Closure cap 2.1 Top 2.2 Lower opening 2.3 Side wall 2.4 Gas passage 2.5 Longitudinal central axis 2.6 Holder 3 Sterile filter 4 Gas supply clamp 4.1 Clamping jaw 4.2 Clamping jaw 4.3 Web 4.4 Recess 4.5 Smooth periphery 4.6 Inlet 4.7 Outlet 4.8 Latching nose 4.9 Groove 4.10 O-Ring 4.11 Lateral wall 5 Shaking incubator 5.1 Interior space 5.2 Shaker 5.3 Conduits 5.4 Gas distributor and gas scrubber 5.5 Gas feed conduit 5.6 Gas mixing station 5.7 Gas sources