DISPOSABLE BIOREACTOR AND USE THEREOF

Abstract

The present invention relates to disposable bioreactors having at least one permanently integrated disposable ATR element. These bioreactors are suitable for cultivating microorganisms or cell cultures, or for cell-free gene expression, wherein liquid samples are examined in the bioreactor by means of infrared spectroscopy.

Claims

1-14. (canceled)

15. A bioreactor for single-use comprising a reactor housing and a silicon-containing ATR element, intended for single-use which is inseparably connected to the reactor housing and which is connectable to an infrared spectrometer.

16. The bioreactor according to claim 15, wherein the ATR element comprises a single reflection element.

17. The bioreactor according to claim 16, wherein the single reflection element comprises one or more microprisms.

18. The bioreactor according to claim 15, wherein the ATR element comprises a multiple reflection element.

19. The bioreactor according to claim 15, wherein the ATR element is an ATR infrared microscopy element.

20. The bioreactor according to claim 15, wherein the ATR element consists of silicon.

21. The bioreactor according to claim 15, wherein the ATR element consists of a silicon substrate with a coating.

22. The bioreactor according to claim 15, wherein the ATR element comprises a round or angular shape.

23. The bioreactor according to claim 15, wherein the ATR element is connected to the side surfaces and/or the bottom of the bioreactor in an air-tight manner.

24. The bioreactor according to claim 15, wherein the infrared spectrometer emits light in the wavelength range from 2 to 20 μm.

25. The bioreactor according to claim 15, wherein the reactor housing is air-tight to prevent contamination.

26. The bioreactor according to claim 15, wherein the reactor housing is designed as a non-dimensionally stable container.

27. The bioreactor according to claim 15, wherein the reactor housing is made from a polymer.

28. The bioreactor according to claim 27, wherein the polymer is selected from the group consisting of polyether ether ketones (PEEK), polycarbonates (PC), polyether sulfones (PES), polysulfones (PS), polyvinyl chlorides (PVC), and combinations thereof.

29. The bioreactor according to claim 15, wherein the bioreactor comprises a sensor or sensors.

30. The bioreactor according to claim 29, wherein the sensor or sensors is/are for determining the pH or oxygen content in the bioreactor.

31. The bioreactor according to claim 29, wherein the sensors are connected airtight to the reactor housing.

32. A method of cultivating of microorganisms or cell cultures or causing cell-free gene expression, the method comprising utilizing the bioreactor according to claim 15 and cultivating of microorganisms or cell cultures or causing cell-free gene expression.

Description

[0027] Based on the following figures, the subject matter of the invention is to be explained in more detail without wanting to restrict it to the specific form shown here.

[0028] FIG. 1 shows a single-use bioreactor according to the invention.

[0029] FIG. 2 shows a detailed view of the single-use bioreactor according to the invention with an integrated ATR element.

[0030] FIG. 1 shows the single-use bioreactor (1) with the permanently integrated disposable ATR element (2). The single-use ATR element is integrated in the figure as an example on the side.

[0031] FIG. 2 shows the detailed view of the single-use bioreactor with a permanently integrated single-use ATR element and the light path within the stated ATR element. The infrared radiation (3) is coupled in and out through the microprisms (5). The interface between the sample in the single-use bioreactor (1) and the single-use ATR element (2), is where the attenuated total reflection occurs.

LIST OF REFERENCE NUMBERS

[0032] Single-use bioreactor [0033] Single-use ATR element [0034] Infrared light [0035] Attenuated total reflection [0036] Microprisms