Composition for improving internal circulation and delaying aging and application thereof

Abstract

The present invention discloses a composition for improving internal circulation and delaying aging and application thereof, and the composition is prepared by Rosa roxburghii, Phyllanthus emblica, honey, walnut and Citrus limon as raw materials.

Claims

1. A therapeutic composition for treating amnesia, insomnia or hair loss in a human in need thereof consisting essentially of 15-50 parts of Rosa roxburghii, 15-50 parts of Phyllanthus emblica, 15-50 parts of honey, 5-20 parts of walnuts and 5-20 parts of Citrus limon.

2. The therapeutic composition of claim 1, which is made by grinding or cutting into slices Rosa roxburghii, Phyllanthus emblica, walnut and Citrus limon and then extracting with alcohol and percolating the resulting mixture, then adding honey to the resulting mixture to produce the composition of claim 1.

3. A method of treating amnesia, insomnia or hair loss in a human in need thereof consisting essentially of administering to the human in need thereof 15-50 parts of Rosa roxburghii, 15-50 parts of Phyllanthus emblica, 15-50 parts of honey, 5-20 parts of walnuts and 5-20 parts of Citrus limon.

Description

DETAILED DESCRIPTION

(1) The present invention will be described more specifically with reference to embodiments. It should be appreciated that implementation of the present invention is not limited to the embodiments below, and various changes and/or modifications made to the present invention in any form will fall within the scope of the present invention.

(2) In the present invention, all parts and percentages are a unit of weight unless otherwise specified. Methods in the following embodiments are conventional methods of the field unless otherwise specified; in the present invention, the Rosa roxburghii, Phyllanthus emblica, walnut and Citrus limon may be fresh or dried products.

(3) Embodiment 1: 100 g Rosa roxburghii, 100 g Phyllanthus emblica, 60 g honey, 30 g walnuts and 15 g Citrus limon were taken and added 5 times of 50° white spirit according to gross weight of the recipe, soaked for 30 d and filtered, a soup was collected.

(4) Embodiment 2: 5 g Rosa roxburghii, 10 g Phyllanthus emblica, 3 g walnuts and 1 g Citrus limon were taken and crushed into coarse powder, soaked by 50° white spirit, and percolated for extraction at 1 ml/min, then, 2 times of percolate was collected according to the weight of the recipe, 5 g honey was added to the percolate for mixing well.

(5) Embodiment 3: 40 g Rosa roxburghii, 40 g Phyllanthus emblica, 30 g honey, 15 g walnut and 10 g Citrus limon were taken and added 4 times of 50° white spirit according to gross weight of the recipe, soaked for 30 d and filtered, a soup was collected.

(6) Embodiment 4: 30 g Rosa roxburghii, 25 g Phyllanthus emblica, 10 g walnuts and 5 g Citrus limon were taken and crushed into coarse powder, soaked by 50° white spirit, and percolated for extraction at 3 ml/min, then, 3 times of percolate was collected according to the weight of the recipe, 20 g honey was added to the percolate for mixing well.

(7) Embodiment 5: 15 g Rosa roxburghii, 10 g Phyllanthus emblica, 10 g walnuts and 5 g Citrus limon were taken and added 6 times of water according to gross weight of the medicinal materials, soaked for half an hour and extracted for twice 2 h each time, and filtered, then an extracting solution is blended and added 10 g honey for mixing well.

(8) Embodiment 6: 15 g Rosa roxburghii, 20 g Phyllanthus emblica, 15 g honey, 1 g walnut and 3 g Citrus limon were taken and added 4 times of water according to gross weight of the recipe, put into a cool place for fermentation for 30 d, then filtered, a fermentation broth was collected.

(9) Embodiment 7: 10 kg Rosa roxburghii, 6 kg Phyllanthus emblica, 6 kg walnuts, 9 kg honey and 3 kg Citrus limon were taken and added 5 times of 50° white spirit according to gross weight of the recipe, soaked for 30 d and filtered, a soup was collected and subpackaged into 100 ml/bottle.

(10) Embodiment 8: 100 kg Rosa roxburghii, 90 kg Phyllanthus emblica, 30 kg walnuts and 25 kg Citrus limon were taken and added 4 times of water according to gross weight of the medicinal materials, soaked for half an hour and extracted for twice 2 h each time, and filtered, then an extracting solution is blended and added 3 kg honey for mixing well, and subpackaged into 100 ml/bottle.

(11) Embodiment 9: 5 kg Rosa roxburghii, 3 kg Phyllanthus emblica, 6 kg walnuts and 6 kg Citrus limon were taken and soaked by 50° white spirit for staying overnight, and percolated for extraction at 20 ml/min, then, 3 times of percolate was collected according to the weight of the recipe, 4 kg honey was added to the percolate for mixing well, and subpackaged into 100 ml/bottle.

(12) Embodiment 10: 6 kg Rosa roxburghii, 6 kg Phyllanthus emblica, 10 kg honey, 2 kg walnuts and 2 kg Citrus limon were taken and added 4 times of water according to gross weight of the recipe, put into a cool place for fermentation for 30 d, then filtered, a fermentation broth was collected, and subpackaged into 100 ml/bottle.

(13) Embodiment 11: Clinical effect of the composition of the present invention

(14) The composition of the present invention may improve internal circulation and enhance body functions better, thus delaying aging by dredging the channel, regulating Qi functions of visceral organs, expelling vivotoxins and supplementing human nutrition. Clinical research data and data of the composition are as follows: 237 cases were selected from March 2011 to November 2016, and the inclusion criteria: patients suffered lassitude, amnesia, intolerance of cold and cold limbs, poor appetite and insomnia, waist-leg weakness, hair loss and loose tooth, short breath and tiredness and other common senile symptoms. Therapies: the composition in Embodiment 10 of the present invention was taken for three times per day, 100 ml each time, a course of treatment lasted 3 weeks and three courses were observed totally, and other anti-aging drugs were withdrawn during administration. Therapeutic outcome:

(15) (1) Overall clinical efficacy: 134 cases were remarkably effective, accounting for 56.54%; 62 cases were effective, accounting for 26.16%; 41 cases were ineffective, accounting for 17.30%. The total effective rate was 196 cases, accounting for 82.70%.

(16) (2) Improvement effect on clinical common senile symptoms was shown in table 1 below:

(17) TABLE-US-00001 TABLE 1 Improvement effect of the composition on common clinical senile symptoms Remarkably effective Effective Ineffective Number Number Percent Number Percent Number Percent Groups of cases of cases age (%) of cases age (%) of cases age (%) Lassitude 215 123 57 60 28 32 15 Waist-leg 217 114 53 51 24 52 24 weakness Amnesia 187 92 49 38 20 57 30 Poor appetite 174 97 56 55 32 22 13 and insomnia Short breath 165 81 49 38 23 46 28 and tiredness Hair loss and 154 76 49 45 29 33 21 loose tooth Intolerance of cold 144 69 48 39 27 36 25 and cold limbs

(18) It can be seen the above table that the composition may improve common various clinical symptoms caused by human aging to different extents, and has remarkable efficacy in enhancement of patients' functions, delay of aging and other aspects.

(19) To sum up, by clinical observation of aging syndrome and lab data analysis, the results show that the composition of the present invention has remarkable efficacy in channel dredging, regulation of Qi functions of visceral organs, expelling of vivotoxins, supplementary of human nutrition, improvement of internal circulation, enhancement of body functions and delay of aging, moreover, the composition has no adverse reaction and side effects.

(20) Embodiment 12: Anti-aging test

(21) The anti-aging effect of the composition of the present invention was researched by a life test for Drosophila melanogaster (provided by Animal Genetics Teaching and Research Office of College of Life Science of Southwest University), a blank control group (distilled water), a control group of incomplete recipe (containing Rosa roxburghii only, containing Phyllanthus emblica and Phyllanthus emblica, containing Rosa roxburghii, Phyllanthus emblica and walnut) and a control group (Embodiment 2 (the solution obtained after removing ethanol), Embodiment 6 and Embodiment 8) were set, average life (an arithmetic average of the total life of Drosophila melanogaster in each group) and maximum life (an arithmetic average of the life of the last surviving 10 Drosophila melanogaster in each group) served as evaluation indexes to evaluate the aging resistance of the composition of the present invention.

(22) Preparation of a basic media: 100 g corn flour, 135 g sucrose, 10 g yeast powder, 1.5 g benzoic acid (dissolved by 4 ml 95% ethanol), 15 g agar and 740 ml distilled water (containing test samples of treatment groups) were prepared into a blank medium without samples and a medium containing 1% of test samples (in the incomplete recipe group, samples in each group were added via a form of converted decoction liquor prepared by corresponding medicinal materials and water; in the treatment group, samples in embodiments were added after converted, and the addition: per 100 g medium contained 1 g raw medicinal materials after converted) for further use.

(23) Treatment and Culture of Drosophila melanogaster

(24) 700 pieces of feathered and non-mated Drosophila melanogaster were collected within 8 h, narcotized by ether and divided into 7 groups, namely, blank control group, Rosa roxburghii group, Rosa roxburghii+Phyllanthus emblica group, Rosa roxburghii+Phyllanthus emblica+walnut group, group of Embodiment 2, group of Embodiment 6 and group of Embodiment 8. There were 100 pieces in each group for half male and half female, respectively put into corresponding culture flasks for culture, and the culture flasks were sealed by plugs prepared by coating gauze on sterilized degreasing cotton, put into a thermostatic incubator (25±1)° C., and the media were replaced every 5 d.

(25) Observation Index

(26) 7 groups were observed at 9: 30 every morning and number of the dead flies was recorded excepting for the dead caused by excessive anesthesia or sticking by media and other human factors. An arithmetic average of the life of the last 10 surviving Drosophila melanogaster served as the maximum life of the group; an arithmetic average of the total life of Drosophila melanogaster in each group served as the average life of the group.

(27) Experimental Results

(28) Influences of samples in each group on the average life and maximum life of Drosophila melanogaster were shown in table 2.

(29) TABLE-US-00002 TABLE 2 Influences of samples in each group on the average life and maximum life of Drosophila melanogaster Number of Average Maximum Drosophila life life Groups melanogaster (d) (d) Blank control 68 59 ± 20.52  85 ± 11.31 Rosa roxburghii 77 65 ± 14.35 91 ± 9.49 Rosa roxburghii + 71 66 ± 8.51   93 ± 12.77 Phyllanthus emblica Rosa roxburghii + 68 64 ± 15.27 94 ± 8.92 Phyllanthus emblica + walnut Embodiment 2 74  75 ± 11.34* 102 ± 7.96* Embodiment 6 79 73 ± 9.68* 105 ± 5.38* Embodiment 8 83  77 ± 12.21* 100 ± 6.82* *Compared with blank control group, it had statistic difference (p < 0.05)

(30) The above researches show that compared with the blank control group and the group of incomplete recipe, the composition of the present invention may remarkably extend the life of Drosophila melanogaster, thereby the composition of the present invention has remarkable effect in aging delaying.

(31) Embodiment 13: Antioxidation Experiment

(32) Oxidation effect of the composition of the present invention was researched by two in-vitro antioxidant models, namely, 1,1-diphenyl picryl phenylhydrazine (DPPH) free radicals and hydroxyl radicals. A blank control group (distilled water), a control group of incomplete recipe (containing Rosa roxburghii only, containing Rosa roxburghii and Phyllanthus emblica, containing Rosa roxburghii, Phyllanthus emblica and walnut) and treatment groups (Embodiment 5, Embodiment 7 (the solution obtained after removing ethanol), Embodiment 10) were set; in the incomplete recipe group, samples in each group were obtained by decocting corresponding medicinal materials and water for concentration (1 ml concentrated liquor was equivalent to 1 g raw medicinal materials); and in treatment groups, samples in embodiments were obtained by concentrating the samples thereof (1 ml concentrated liquor was equivalent to 1 g raw medicinal materials), and the experimental method was as follows:

(33) Measurement of Scavenging Activity of DPPH Free Radicals:

(34) Samples were taken and diluted by distilled water for 10 times to obtain the samples to be tested. 0.2 mL samples to be tested were exactly taken to test tubes, 2.0 mL (0.4 mmol/L) DPPH and 70% ethanol solution were added for oscillation evenly, and the 2.0 mL (0.4 mmol/L) DPPH and 70% ethanol solution were mixed as a blank control group, reaction was performed in a dark place at room temperature, then absorbance values were measured at 517 nm wavelength, and 70% ethanol served as a reference for zero setting. Calculation method of the clearance rate of DPPH free radicals:

(35) $Y=\frac{A_0-A}{A_0}\times 100\%$

(36) in the formula: A.sub.0 is an absorbance value of the control sample at 517 nm; A is an absorbance value of the sample.

(37) Measurement of Scavenging Activity of Hydroxyl Radicals:

(38) Samples were taken and diluted by distilled water for 10 times to obtain the samples to be tested. 0.25 mL FeSO4 (2 mmol/L), 0.5 mL salicylic acid (6 mmol/L) and 1 mL samples to be tested were successively added to a reaction system for mixing well, then 0.25 mL H.sub.2O.sub.2 (mass fraction: 0.01%) was added. Reaction was performed for 30 min at 37° C. for cooling, and then absorbance was measured at 510 nm. Calculation method of the clearance rate (%) of hydroxyl radicals:

(39) ${}_{\mathrm{••}}Z=\frac{Z_0-\left(Z_i-Z_{i0}\right)}{Z_0}\times 100\%$

(40) in the formula: Z.sub.0 is an absorbance value of the blank control group; Z.sub.i is an absorbance value of the group with samples; Z.sub.i0 is an absorbance value of the group without H.sub.2O.sub.2, a color developing agent.

(41) Experimental results are shown in Table 3.

(42) TABLE-US-00003 TABLE 3 Results of the in-vitro antioxidant experiment DPPH Clearance ratio of clearance ratio hydroxyl radicals Groups (%) (%) Blank control 0 0 Rosa roxburghii 56.52 ± 4.63  64.71 ± 5.32  Rosa roxburghii + 63.11 ± 7.02  68.93 ± 6.71  Phyllanthus emblica Rosa roxburghii + 73.24 ± 4.93  79.24 ± 8.45  Phyllanthus emblica + walnut Embodiment 5 79.74 ± 11.28 83.76 ± 12.87 Embodiment 7 93.28 ± 10.84 97.31 ± 12.49 Embodiment 10 85.02 ± 11.32 89.09 ± 12.91 *Each experiment was repeated for three times.

(43) Results of the antioxidation experiment show that compared with the blank control group and the group of incomplete recipe, the composition of the present invention may remarkably clear away DPPH free radicals and hydroxyl radicals, thereby the composition of the present invention has remarkable effect in antioxidation.