Method for promoting growth and preservation quality of <i>Agaricus bisporus</i>

Abstract

The present invention provides a method for promoting the growth and preservation quality of Agaricus bisporus, and belongs to the technical field of cultivation and preservation of edible fungi. In the present invention, the methyl jasmonate solution is sprayed onto the surface of Agaricus bisporus at a concentration of 10-200 μmol/L when the Agaricus bisporus grows to the mushroom bud stage, which can accelerate the growth and development of the Agaricus bisporus, improve the post-harvest preservation quality of the Agaricus bisporus, and prolong the shelf life thereof, then improving the yield and commercial value of the Agaricus bisporus. The present invention provides a new method for increase in both production and income and post-harvest preservation of Agaricus bisporus, can be applied to large-scale industrial production of Agaricus bisporus, and has important economic benefits and application promotion value.

Claims

1. A method for promoting the growth and preservation quality of Agaricus bisporus, comprising spraying a methyl jasmonate solution of 10-50 μmol/l onto the surface of Agaricus bisporus when Agaricus bisporus grows to a mushroom bud stage.

2. The method according to claim 1, wherein the solvent of the methyl jasmonate solution is an ethanol-water mixed solvent; and the volume concentration of ethanol in the ethanol-water mixed solvent is 1.5-2.5%.

3. The method according to claim 1, wherein the amount of the methyl jasmonate solution sprayed onto the surface of Agaricus bisporus is 20-40 mL/m.sup.2 mushroom bed.

4. The method according to claim 1, after the spraying, further comprising the step of culturing Agaricus bisporus to the harvest time of Agaricus bisporus.

5. The method according to claim 4, wherein the culturing temperature is 16-18° C.

6. The method according to claim 4, wherein the culturing humidity is 88-92%.

7. The method according to claim 2, after the spraying, further comprising the step of culturing Agaricus bisporus to the harvest time of Agaricus bisporus.

8. The method according to claim 7, wherein the culturing temperature is 16-18° C.

9. The method according to claim 7, wherein the culturing humidity is 88-92%.

10. The method according to claim 3, after the spraying, further comprising the step of culturing Agaricus bisporus to the harvest time of Agaricus bisporus.

11. The method according to claim 10, wherein the culturing temperature is 16-18° C.

12. The method according to claim 10, wherein the culturing humidity is 88-92%.

Description

BRIEF DESCRIPTION OF THE DRAWING

(1) FIG. 1 is a diagram showing the result of growth-situation comparison between Agaricus bisporus treated by the method described in Embodiment 1 of the present invention and Agaricus bisporus as treated in a blank group; and

(2) FIG. 2 is a diagram showing the result of post-harvest storage situation comparison between Agaricus bisporus treated by the method described in Embodiment 1 of the present invention and Agaricus bisporus as treated in a blank group.

DETAILED DESCRIPTION

(3) The present invention provides a method for promoting the growth and preservation quality of Agaricus bisporus, including spraying a methyl jasmonate solution of 10-200 μmol/l onto the surface of Agaricus bisporus when Agaricus bisporus grows to a mushroom bud stage.

(4) In the present invention, the methyl jasmonate (MeJA) solution is sprayed onto the surface of Agaricus bisporus when the Agaricus bisporus grows to the mushroom bud stage. In the present invention, the concentration of the MeJA solution is preferably 10-200 μmol/L, more preferably 20-150 μmol/L, and even more preferably 50-100 μmol/L. The solvent of the MeJA solution is preferably an ethanol-water mixed solvent; and the volume concentration of ethanol in the ethanol-water mixed solvent is preferably 1.5-2.5%, and more preferably 2%. In the present invention, the MeJA solution is used as an in vitro inducer of Agaricus bisporus, which can effectively improve the growth rate of Agaricus bisporus, increase the yield of Agaricus bisporus, advance the harvest time of Agaricus bisporus, and prolong the post-harvest storage period of it.

(5) In the present invention, the amount of the MeJA solution sprayed onto the surface of Agaricus bisporus is based on the area of a mushroom bed, and is preferably 20-40 mL/m.sup.2, more preferably 25-35 mL/m.sup.2, and most preferably 30 mL/m.sup.2. Agaricus bisporus treated with such a spraying amount has the best growth rate and freshness.

(6) In the present invention, after the MeJA solution is sprayed onto the surface of Agaricus bisporus, it preferably further includes the step of culturing Agaricus bisporus to the harvest time of it. In the present invention, the culturing temperature is preferably 16-18° C., and more preferably 17° C. The culturing humidity is preferably 88-92%, and more preferably 90%. By culturing the Agaricus bisporus sprayed with the MeJA solution to mature according to the method provided by the present invention, the MeJA can exert better effect of promoting the growth and preservation quality.

(7) The method for promoting the growth and preservation quality of Agaricus bisporus as provided by the present invention will be described in detail below in connection with Embodiments, but these Embodiments should not be understood as limiting the claimed scope of the present invention.

Embodiment 1

(8) Experiment of Promoting the Growth and Preservation Quality of Agaricus bisporus

(9) (1) An ethanol-water solvent with an ethanol concentration (by volume fraction) of 2% was prepared, and then the methyl jasmonate (MeJA) was dissolved in the ethanol-water solvent to prepare MeJA solutions at concentrations respectively of 10, 50, 100, and 200 μmol/L.

(10) (2) The aforementioned MeJA solutions were each used as an inducer and poured into an automatic spraying device, and were each uniformly sprayed onto the surface of Agaricus bisporus when it was grown to the mushroom bud stage, where the spraying dose of 30 mL/m.sup.2 was used as the treatment group. At the same time, the treatments with water and a 2% ethanol solution were as a blank group and a control group, respectively. The subsequent culturing conditions were 17° C. and a humidity of 90%.

(11) (3) Photographing and sampling are performed every 24 h during the treatment. The photograph comparison results were shown in FIG. 1. After sampling, the weight (m) of a single mushroom was weighed and recorded, and the diameter of pileus (a), the diameter of stipe (b) and the thickness of pileus (c) were measured with a microcalliper and recorded to calculate the average values, and the results were shown in Table 1.

(12) TABLE-US-00001 TABLE 1 Effect of the method provided by the present invention on the growth properties of Agaricus bisporus Diameter of Diameter of Thickness of Weight (g) Pileus (mm) Stipe (mm) Pileus (mm) on the day 0.57 ± 0.01  9.77 ± 0.16 — — of treatment day 1 after blank group 3.51 ± 0.06 19.66 ± 0.71 14.65 ± 0.46 11.52 ± 0.45 treatment 2% ethanol 3.48 ± 0.08 20.47 ± 0.81 14.02 ± 0.98 11.98 ± 0.69  10 μM MeJA 3.83 ± 0.01 20.21 ± 0.67 12.60 ± 0.91 12.07 ± 0.48  50 μM MeJA 6.81 ± 0.09 24.29 ± 0.75 13.91 ± 0.66 14.80 ± 0.49 100 μM MeJA 3.69 ± 0.06 20.52 ± 0.33 14.14 ± 0.35 12.28 ± 0.32 200 μM MeJA 3.48 ± 0.14 20.79 ± 0.57 13.56 ± 0.83 12.70 ± 0.19 day 2 after blank 10.84 ± 0.40  32.19 ± 0.31 17.37 ± 0.67 17.70 ± 0.22 treatment 2% ethanol 10.81 ± 0.35  31.38 ± 1.01 17.32 ± 0.85 19.97 ± 1.08  10 μM MeJA 16.27 ± 0.27  37.04 ± 0.47 18.17 ± 0.60 21.49 ± 0.50  50 μM MeJA 20.34 ± 0.20  39.01 ± 0.67 18.76 ± 0.38 22.29 ± 0.21 100 μM MeJA 11.18 ± 0.10  31.97 ± 0.78 16.30 ± 0.82 18.73 ± 0.82 200 μM MeJA 10.10 ± 0.14  30.71 ± 0.69 17.94 ± 0.39  17.5 ± 0.48

(13) The Agaricus bisporus was stored under conditions of 4° C. and a relative humidity of 90-95% after harvested, so as to observe its appearance and quality, and the results were as shown in FIG. 2.

(14) It can be seen from FIG. 1 and Table 1 that, as compared with the blank group and the group treated with ethanol, the treatment with methyl jasmonate at the concentration of 10-100 μmol/L has a significant effect of promoting the growth of Agaricus bisporus.

(15) It can be seen from FIG. 2 that, as compared with the blank group and the group treated with ethanol, the treatment with methyl jasmonate at the concentration of 10-200 μmol/L has a significant preserving effect on Agaricus bisporus.

(16) The foregoing descriptions are only preferred implementation manners of the present invention. It should be noted that for a person of ordinary skill in the art, several improvements and modifications may further be made without departing from the principle of the present invention. These improvements and modifications should also be deemed as falling within the protection scope of the present invention.