Species of Genus Pholiota and Artificial Cultivation Method and Use Thereof

Abstract

The invention relates to a rare edible fungus new strain and an artificial cultivation method and use thereof, in particular to a new species Pholiota glutinosior of genus Pholota and its artificial cultivation method and use. The new strain of genus Pholiota of the present invention is collected from the Guangdong Chebaling National Nature Reserve, and is identified as a new strain of genus Pholiota, and the original strain is obtained by tissue separation, and is named as HMGIM-W140054, deposited on Jun. 3, 2019, at China Center for Type Culture Collection (CCTCC, Wuhan, China), with the accession number CCTCC NO: M 2019414. The new strain of the invention has been artificially domesticated and cultivated, exhibits a strong and significant inhibition rate against Staphylococcus, and has a high content of histidine, crude polysaccharide and potassium. It is rich in nutrients and has high cultivation yield, and is a new species with high development prospects.

Claims

1. A new species of genus Pholiota, Pholiota glutinosior, comprising HMGIM-W140054, and having an accession number of CCTCC NO: M 2019414.

2. An artificial cultivation method of the new species of genus Pholiota glutinosior of claim 1, comprising: producing a first-class strain, producing a second-class strain, producing a third-class strain, cultivating a culture having a cultivating medium in a cultivation bag, wherein by weight percent, the cultivating medium comprises 28-32% cottonseed hulls, 56-58% wood chips, 8-12% bran, and 1-2% CaCO.sub.3.

3. The artificial cultivation method according to claim 2, wherein by weight percent, the cultivating medium comprises 31% cottonseed hulls, 58% wood chips, 10% bran, 1% CaCO.sub.3, and has a moisture content of 60% to 65%.

4. The artificial cultivation method according to claim 3, wherein the cultivation comprises: transferring the third-class strain to the cultivating medium, keeping constant temperature at 25-26° C., shading the culture, keeping relative humidity at 60%-70%, and a cultivation management stage is entered when hyphae have overgrown the cultivation bag.

5. The artificial cultivation method according to claim 4, wherein the cultivation management stage comprises: after the hyphae in the cultivation bag is full of the cultivating medium, the cultivation continues for 25 days after the shading to grow a mushroom with a cap, temperature is controlled at 18-20° C., and ventilation is increased to keep space carbon dioxide content below 1%; the relative humidity is adjusted to more than 90%; after 14 days, the mushroom cap is removed and the hyphae begins to kink and form a pale yellow rice bran-like primordium; after the primordium grows to 0.5 cm, the temperature is kept at 18-20° C., relative humidity between 80-90%, exposure to 9 hours of light per day of light intensity 300-500 lx, and keeping carbon dioxide concentration in the air 350˜1500 ppm, after 8 days, spraying the mushroom every day 1-2 times until the size of the mushroom fruit body is unchanged, and the fruit body cap no longer grows and begins to flatten, then harvesting the mushroom.

6. The artificial cultivation method according to claim 2, wherein production of the first-class strain comprises: transferring isolated strains to a first-class strain culture medium, and placing the first-class strain culture medium in a constant temperature dark culture at 25° C.; when mycelium grows and bacteria have not grown, tip hyphae of the mycelium are picked up to obtain the first-class strain; or, production of the second-class strain comprises: transferring the first-class strain to a second-class strain culture medium, and placing the second-class strain culture medium in a constant temperature dark culture at 25° C., and the mycelium grows full of slopes to obtain the second-class strain; or, production of the third-class strain comprises: inoculating the second-class strain into a third-class strain culture medium by ensuring that the second-class strain culture medium is buried in the first-class strain culture medium and is placed in a constant temperature dark culture at 25° C. until the hyphae are full of the third-class strain culture medium, to obtain the third-class strain.

7. The artificial cultivation method according to claim 3, wherein production of the first-class strain comprises: transferring isolated strains to a first-class strain culture medium, and placing the first-class strain culture medium in a constant temperature dark culture at 25° C.; when mycelium grows and bacteria have not grown, tip hyphae of the mycelium are picked up to obtain the first-class strain; or, production of the second-class strain comprises: transferring the first-class strain to a second-class strain culture medium, and placing the second-class strain culture medium in a constant temperature dark culture at 25° C., and the mycelium grows full of slopes to obtain the second-class strain; or, production of the third-class strain comprises: inoculating the second-class strain into a third-class strain culture medium by ensuring that the second-class strain culture medium is buried in the first-class strain culture medium, and is placed in a constant temperature dark culture at 25° C. until the hyphae are full of third-class strain culture medium, to obtain the third-class strain.

8. The artificial cultivation method according to claim 4, wherein production of the first-class strain comprises: transferring isolated strains to a first-class strain culture medium, and placing the first-class strain culture medium in a constant temperature dark culture at 25° C.; when mycelium grows and bacteria have not grown, tip hyphae of the mycelium are picked up to obtain the first-class strain; or, production of the second-class strain comprises: transferring the first-class strain to a second-class strain culture medium, and placing the second-class strain culture medium in a constant temperature dark culture at 25° C., and the mycelium grows full of slopes to obtain the second-class strain; or, production of the third-class strain comprises: inoculating the second-class strain into a third-class strain culture medium by ensuring that the second-class strain culture medium is buried in the first-class strain culture medium, and is placed in a constant temperature dark culture at 25° C. until the hyphae are full of third-class strain culture medium, to obtain the third-class strain.

9. The artificial cultivation method according to claim 5, wherein production of the first-class strain comprises: transferring isolated strains to a first-class strain culture medium, and placing the first-class strain culture medium in a constant temperature dark culture at 25° C.; when mycelium grows and bacteria have not grown, tip hyphae of the mycelium are picked up to obtain the first-class strain; or, production of the second-class strain comprises: transferring the first-class strain to a second-class strain culture medium, and placing the second-class strain culture medium in a constant temperature dark culture at 25° C., and the mycelium grows full of slopes to obtain the second-class strain; or, production of the third-class strain comprises: inoculating the second-class strain into a third-class strain culture medium by ensuring that the second-class strain culture medium is buried in the first-class strain culture medium, and is placed in a constant temperature dark culture at 25° C. until the hyphae are full of third-class strain culture medium, to obtain the third-class strain.

10. The artificial cultivation method according to claim 6, wherein the first-class strain culture medium is Bengal red medium comprising, by weight percent: peptone 0.5%, glucose 1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate (MgSO.sub.4.7H.sub.2O) 0.05%, agar 2%, 1/3000 Bangladesh Red solution 10%, chloramphenicol 0.01%, and the remainder is water; or, the second-class strain culture medium is an enriched integrated potato dextrose agar, comprising, by weight percent: 20% potato, 20% glucose, 1% peptone, 2% agar, 0.3% potassium dihydrogen phosphate, 0.15% magnesium sulfate, 0.15% vitamin B1, and the remainder is water; by weight percent, the third-class strain culture medium comprises: 98-99% sorghum and 1-2% calcium carbonate.

11. The artificial cultivation method according to claim 7, wherein the first-class strain culture medium is Bengal red medium comprising, by weight percent: peptone 0.5%, glucose 1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate (MgSO.sub.4.7H.sub.2O) 0.05%, agar 2%, 1/3000 Bangladesh Red solution 10%, chloramphenicol 0.01%, and the remainder is water; or, the second-class strain culture medium is an enriched integrated potato dextrose agar, comprising, by weight percent: 20% potato, 20% glucose, 1% peptone, 2% agar, 0.3% potassium dihydrogen phosphate, 0.15% magnesium sulfate, 0.15% vitamin B1, and the remainder is water; by weight percent, the third-class strain culture medium comprises: 98-99% sorghum and 1-2% calcium carbonate.

12. The artificial cultivation method according to claim 8, wherein the first-class strain culture medium is Bengal red medium comprising, by weight percent: peptone 0.5%, glucose 1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate (MgSO.sub.4.7H.sub.2O) 0.05%, agar 2%, 1/3000 Bangladesh Red solution 10%, chloramphenicol 0.01%, and the remainder is water; or, the second-class strain culture medium is an enriched integrated potato dextrose agar, comprising, by weight percent: 20% potato, 20% glucose, 1% peptone, 2% agar, 0.3% potassium dihydrogen phosphate, 0.15% magnesium sulfate, 0.15% vitamin B1, and the remainder is water; by weight percent, the third-class strain culture medium comprises: 98-99% sorghum and 1-2% calcium carbonate.

13. The artificial cultivation method according to claim 9, wherein the first-class strain culture medium is Bengal red medium comprising, by weight percent: peptone 0.5%, glucose 1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate (MgSO.sub.4.7H.sub.2O) 0.05%, agar 2%, 1/3000 Bangladesh Red solution 10%, chloramphenicol 0.01%, and the remainder is water; or, the second-class strain culture medium is an enriched integrated potato dextrose agar, comprising, by weight percent: 20% potato, 20% glucose, 1% peptone, 2% agar, 0.3% potassium dihydrogen phosphate, 0.15% magnesium sulfate, 0.15% vitamin B1, and the remainder is water; by weight percent, the third-class strain culture medium comprises: 98-99% sorghum and 1-2% calcium carbonate.

14. Use of the Pholiota glutinosior CCTCC NO: M 2019414 of claim 1 or an extract thereof for treating bacteria, wherein the Pholiota glutinosior or the extract are used for preparation of the medicament for treating diseases caused by anti-bacteria or for preparation of a health supplement; the extract is preferably ethyl acetate extract; the bacteria is Staphylococcus, preferably Staphylococcus aureus; the health supplement is rich in histidine, or crude polysaccharide, or potassium content.

15. The medicament for treating diseases caused by anti-bacteria of claim 14, comprising the Pholiota glutinosior CCTCC NO: M 2019414 or the extract thereof and a carrier.

Description

BRIEF DESCRIPTION OF DRAWINGS

[0025] FIG. 1 is wild fruiting body of Pholiota glutinosior CCTCC NO: M 2019414 of Example 1.

[0026] FIG. 2 is a photograph of Basidiospores under electron microscope of Pholiota glutinosior CCTCC NO: M 2019414 of Example 1.

[0027] FIG. 3 is a photograph of Basidia and Basidiospores under general optical microscope of Pholiota glutinosior CCTCC NO: M 2019414 of Example 1.

[0028] FIG. 4 is a phylogenetic tree constructed by the BI method based on ITS of Example 1.

[0029] FIG. 5 is a phylogenetic tree constructed by the ML method based on ITS of Example 1.

[0030] FIG. 6 is a phylogenetic tree constructed by the NJ method based on ITS of Example 1.

[0031] FIG. 7 is a phylogenetic tree constructed by the BI method based on the LSU of Example 1.

[0032] FIG. 8 is a phylogenetic tree constructed by the BI method based on RPB2 of Example 1.

[0033] FIG. 9 is a fruit body diagram of artificially domesticated new species of genus Pholiota glutinosior of Example 2.

[0034] FIG. 10 is another diagram of artificially domesticated new species of genus Pholiota glutinosior of Example 2.

[0035] FIG. 11 is inhibitory zone of the strain W140054 of the present invention of Example 3.

[0036] In FIG. 11, the upper left is a negative control; the upper right is a positive control; the lower right is the strain W140054 of the present invention.

DETAILED DESCRIPTION

[0037] The Following is further illustration with the specific examples.

[0038] In the first aspect, the new strain of genus Pholiota of the present invention is collected from the Guangdong Chebaling National Nature Reserve, and is identified as a new strain of genus Pholiota, and the original strain is obtained by tissue separation, and is named as HMGIM-W140054, deposited on Jun. 3, 2019, at China Center for Type Culture Collection (CCTCC, Wuhan, China), with the accession number CCTCC NO: M 2019414.

[0039] The ITS sequence of its fruiting body has the highest similarity with Pholiota limonella (Registration Number: KM496470, From Korea), which is 99.2-99.7%, and has the 98.5-98.7% similarity with Pholiota adiposa (Registration Number: FJ810180, From China). Combined with the morphological identification, the macroscopic morphology of the strain is not consistent with Pholiota limonella and Pholiota adiposa. The difference is large. The shape of Pholiota adiposa is larger than that of the present invention more than 2 times, the Basidiospores of Pholiota limonella have obvious bud holes, but the species of the invention do not have bud holes, and Basidiospores of this species are smaller than Pholiota limonella. Therefore, it was judged that the strain of the present invention is a new species different from the current species of genus Pholiota, and was named as Pholiota glutinosior.

[0040] The macroscopic morphology and microscopic morphology of Pholiota glutinosior of the present invention are as follows: Basidiomata small sized. Pileus (0.65)1.0-2.2 cm in diameter, convex to bell shape when young and the edge of the pileus becoming flat, Orange-yellow, the middle color is darker, no obvious mucus, but it has strong viscous. The middle part of the cap has yellow-brown small spots, and the edge of the cap is wound inside, leaving white fluffy flakes. Stipe. central, 1.1˜4.8 cm, Stick-shaped, fleshy, pale yellow to white, nearly equal in thickness, slightly enlarged and curved at the base, with villiform annulus; the stipe above the annulus is smooth and nearly white, and the stipe below the annulus is light yellow and covered with fluff flakes. Hymenophore. lamella white to off-white color, adnate, sparse, unequal length with thickness is about 0.8˜1.0 mm. Basidiospores. 5.57˜7.11×3.46˜4.38 μm, Q=1.68, long-oval, smooth, with yellowish brown color, partially with oily, bud germ pores are not obvious, non-amyloid. Basidia. 17.8˜25.24×6.8˜7.52 μm, clavate without basal clamp connection, sterigmata up to 23 μm. Cheilocystidia. 16.35˜28.24×7.15˜10.0 μm, long stick shape, smooth without attachments. Pleurocystidia. 18.71˜26.1×6.47˜8.62 μm, short rod to spindle with a short-pointed. Clamp connections were present in tissue of context.

[0041] In the second aspect, the present invention provides an artificial cultivation method of Pholiota glutinosior CCTCC NO: M 2019414, which comprises producing the first-class strain, producing the second-class strain, producing the third-class strain, cultivating culture and cultivation management, by weight percent, the cultivating medium comprises 28-32% cottonseed hulls, 56-58% wood chips, 8-12% bran, 1-2% CaCO.sub.3.

[0042] Preferably, the above cultivating medium has moisture content of 60%-65%.

[0043] Preferably, by weight percent, the cultivating medium comprises 31% cottonseed hulls, 58% wood chips, 10% bran, 1% CaCO.sub.3, and has moisture content of 60% to 65%.

[0044] Preferably, the cultivation comprises: transferring the third-class strain to the cultivating medium, keeping constant temperature at 25-26° C., shading culture, humidity 60%-70%, and the hyphae overgrowing the cultivation bag means it is entering into cultivation management.

[0045] Preferably, the cultivation management comprises:

[0046] after the hyphae in the bag is full of the cultivating medium in the bag, the cultivation continues for 25 days after the shading, and entering the mushroom stage, the temperature is controlled at 18-20° C., and the ventilation is increased to keep the space carbon dioxide content below 1%. The relative humidity of the air is adjusted to more than 90%. After 14 days, the cap is removed and the hyphae begins to kink and forms the pale yellow rice bran-like primordium. After the primordium grows to 0.5 cm, the temperature is kept at 18-20° C., relative humidity between 80-90%, 9 hours of light per day, light intensity 300-500 lx, and keep the carbon dioxide concentration in the air 350˜1500 ppm, keep the air moist, after about 8 days, during this period, spraying the mushroom every day 1-2 times until the size of the fruit body is basically unchanged, and the fruit body cap no longer grows and begins to flatten, then harvest.

[0047] Preferably, the preparation of the first-class strain comprises: transferring the isolated strains to the first-class strain culture medium, and placing them in a constant temperature dark culture at 25° C. When the mycelium grows and the bacteria have not grown, the tip hyphae is picked up to obtain the first-class strain.

[0048] Preferably, the first-class strain culture medium is Bengal red medium.

[0049] Further preferably, by weight percent, the Bengal red medium comprises: peptone 0.5%, glucose 1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate (MgSO.sub.4.7H.sub.2O) 0.05%, agar 2%, 1/3000 Bangladesh Red solution 10%, and chloramphenicol 0.01%, the rest is water.

[0050] Preferably, the production of the second-class strain comprises: transferring the first-class strain to the second-class strain culture medium, and placing it in a constant temperature dark culture at 25° C., and the mycelium is full of slopes as to obtain the second-class strain

[0051] Preferably, the second-class strain is an enriched integrated potato dextrose agar.

[0052] Further preferably, by weight percent, the enriched integrated potato dextrose agar comprises: 20% potato, 20% glucose, 1% peptone, 2% agar, 0.3% potassium dihydrogen phosphate, 0.15% magnesium sulfate, and 0.15% vitamin B1, and the rest is water.

[0053] Preferably, the production of the third-class strain comprises: inoculating the second-class strain into the third-class strain culture medium, ensuring that the second-class strain material block is buried in the first-class strain culture medium, and is placed in a constant temperature dark culture at 25° C. until the hyphae full of medium, the third-class strain is obtained.

[0054] Preferably, by weight percent, the third-class strain culture medium comprises: 98-99% sorghum and 1-2% calcium carbonate.

[0055] Further preferably, by weight percent, the third-class strain culture medium comprises: 98% sorghum and 2% calcium carbonate.

[0056] Preferably, the artificial cultivation method further comprises tissue strain isolation before the preparation of the first-class strain.

[0057] An artificial cultivation method of Pholiota glutinosior CCTCC NO: M 2019414, which comprises producing the first-class strain after tissue isolation, producing the first-class strain, producing the second-class strain, producing the third-class strain, cultivating culture and cultivation management, by weight percent, the cultivating medium comprises 28-32% cottonseed hulls, 56-58% wood chips, 8-12% bran, and 1-2% CaCO.sub.3.

[0058] Preferably, the tissue-separating strain comprises: wiping the surface of fruit body with alcohol under aseptic conditions, tearing, and vaccinating the internal tissue of 0.2-0.5 mm×0.2-0.5 mm aseptically, placing in a constant temperature dark culture at 25° C., and the isolated strain was obtained after the slant were covered with hyphae.

[0059] Preferably, the tissue isolation medium is the integrated potato dextrose agar medium.

[0060] Further preferably, by weight percent, the integrated potato dextrose agar medium comprises 20% potato, 2% glucose, 2% agar, 0.3% potassium dihydrogen phosphate, 0.15% magnesium sulfate, and a trace amount of vitamin B1.

[0061] In the third aspect, the present invention provides the use of a new species Pholiota glutinosior CCTCC NO: M 2019414 or an extract thereof for use in diseases associated with anti-bacteria.

[0062] Preferably, the extract is the ethyl acetate extract.

[0063] Preferably, the bacterium is Staphylococcus, further preferably Staphylococcus aureus.

[0064] In the fourth aspect, the present invention provides the use of a new specie Pholiota glutinosior CCTCC NO: M 2019414 or an extract thereof for preparation of a medicament for treating diseases caused by anti-bacteria or for preparation of the health supplement.

[0065] Preferably, the extract is the ethyl acetate extract.

[0066] Preferably, the bacterium is Staphylococcus, further preferably Staphylococcus aureus.

[0067] Preferably, for the preparation of the health supplement comprises for the preparation of health supplement rich in histidine, and/or crude polysaccharide, and/or potassium content.

[0068] In the fifth aspect, the present invention provides the medicament for treating diseases caused by anti-bacteria, comprising a new specie Pholiota glutinosior CCTCC NO: M 2019414 or an extract thereof and a carrier.

[0069] Preferably, the extract is the ethyl acetate extract.

[0070] Preferably, the bacterium is Staphylococcus, further preferably Staphylococcus aureus.

[0071] In the sixth aspect, the present invention provides the health supplement comprising a new specie Pholiota glutinosior CCTCC NO: M 2019414 or an extract thereof.

[0072] Preferably, the health supplement is the health supplement rich in histidine, and/or crude polysaccharide, and/or potassium.

Example 1: Identification of New Strain

[0073] On Mar. 22, 2014, Hu Huiping and Liu Yuanchao collected and investigated large-scale fungal resources in the Chebaling National Nature Reserve, Guangdong Province. A specimen of genus Pholiota was collected on the dead wood, as shown in FIG. 1. FIG. 2 and FIG. 3 are micrographs of the structure, and the original strain was obtained by tissue isolation, named HMGIM-W140054, deposited on Jun. 3, 2019, at China Center for Type Culture Collection (CCTCC, Wuhan, China), with the accession number CCTCC NO: M 2019414.

[0074] Since it did not find the species of genus Pholiota that was consistent with its description in the macroscopic and microscopic morphology, in order to further confirm its classification, DNA extraction was performed, and the genes of the multiple fragments were sequenced, including the fungal ribosomal intergenic region ITS, ribosomal large subunit LSU, a second large subunit RPB2 encoding RNA polymerase II, and the like, which are used for fungal classification of polygenic fragments.

[0075] The researchers in the Edible Fungus Research and Development Center of the Guangdong Institute of Microbiology conducted a low-temperature (35° C.) drying of wild fruiting bodies, grinded by liquid nitrogen, extracted the DNA genome using the Ezup column fungal genomic DNA extraction kit. The DNA solution was chilled at −20° C. for use. Universal primer for fungal ribosomal intergenic region (ITS) ITS1/ITS4 (ITS1: TCC GTA GGT GAA CCT GCG G, ITS4: TCC TCC GCT TAT TGA TAT GC, ribosomal large subunit (LSU) primer LROR/LR7 (LROR): ACC CGC TGA ACT TAA GC, LR7: TAC TAC CAC CAA GAT CT), the second large subunit (RPB2) primer encoding RNA polymerase II RPB2-B-F1/RBP2-BR (RPB2-B-F1: AAG ATY GCY AAG CCT CGT CA, RBP2-BR: AAG ATR TTG GCC ATS GTG TCC) (synthesized by Shanghai Meiji Biomedical Technology Co., Ltd.) PCR experiments on materials, amplification on Biometra PCR instrument, PCR reaction composition (50μl total) is:

[0076] TaKaRaTaq (5 units/μl) 0.25 μl

[0077] 10×PCR Buffer 5 μl

[0078] dNTP Mixture (2.5 mM each) 4 μl

[0079] DNA template 2 μl

[0080] Primer 1 (10 μmol.Math.L−1) 5 μl

[0081] Primer 2 (10 μmol.Math.L−1) 5 μl

[0082] Sterilized distilled water 28.75 μl

[0083] The ITS-PCR reaction condition is: reaction at 94° C. for 5 min; reaction at 94° C. for 1 min, reaction at 55° C. for 1 min, reaction at 72° C. for 1 min, 30 cycles; reaction at 72° C. for 10 min. The PCR product was directly sent for bidirectional sequencing and was completed by Shanghai Meiji Biomedical Technology Co.

[0084] Due to the lack of bases in ITS-PCR, Shanghai Meiji Biomedical Technology Co., Ltd. was further commissioned to carry out cloning and sequencing, and six clones and their sequencing results were obtained. Three sequences were uploaded to Genebank, and the registration serial numbers were MN582988 (SEQ ID NO: 1), MN582989 (SEQ ID NO: 2), and MN582990 (SEQ ID NO: 3).

[0085] The reaction condition of LSU-PCR is: reaction at 95° C. for 3 min; reaction at 94° C. for 30 s, reaction at 50° C. for 45 s, reaction at 72° C. for 2 min, 36 cycles; reaction at 72° C. for 10 min. The obtained LSU sequence was uploaded to Genebank with the registration serial number MN582991 (SEQ ID NO: 4).

[0086] The reaction condition of RPB2-PCR is: reaction at 95° C. for 3 min; reaction at 95° C. for 30 s, reaction at 55° C. for 45 s, reaction at 72° C. for 1.5 min, 36 cycles; reaction at 72° C. for 10 min. The three RPB2 sequences obtained were uploaded to Genebank with accession numbers MN628554 (SEQ ID NO: 5), MN628555 (SEQ ID NO: 6), MN628556 (SEQ ID NO: 7).

[0087] The newly-generated sequences were checked and then submitted to GenBank. Phylogenetic trees were built separately based on ITS, LSU and RPB2, Kuehneromyces mutabilis and Hypholoma fasciculare were selected as outgroup respectively. All sequences were aligned with CLUSTAL W (v.1.83) using default settings. Phylogenetic analysis of ITS, LSU and RBP2 were performed using Bayesian algorithm (BI), the parameters was set as follows: the analysis was run in MrBayes 3.1.2 (Ronquist & Huelsenbeck, 2003), mcmcp ngen=1000000, sample freq.=100. The NJ and ML Phylogenetic trees of ITS were also performed using MEGA (V10.0.4).

[0088] Phylogenetic trees of ITS: Kuehneromyces mutabilis data (AY354218) were choosed as outgroup, phylogenetic trees was constructed on ITS data. The ITS data included sequences from fifteen Pholiota samples representing seven taxa. The best model selected and applied in the Mybayes analysis for the ITS model. BI analysis resulted with an average standard deviation of split frequencies=0.006934. Sequences of the fruit body of HMGIM W140054 had been deposited in GenBank and accession numbers is MN582988, MN582989 and MN582990.

[0089] Phylogenetic trees of LSU: Kuehneromyces mutabilis data (MH866740) were choosed as outgroup, phylogenetic trees was constructed on LSU data. The LSU data included sequences from twelve Pholiota samples representing seven taxa. The best model selected and applied in the Mybayes analysis for the ITS model. BI analysis resulted with an average standard deviation of split frequencies=0.003046. Sequences of the fruit body of HMGIM W140054 had been deposited in GenBank and accession numbers is MN582991.

[0090] Phylogenetic trees of RPB2: Hypholoma fasciculare data (AY337413) were choosed as outgroup, phylogenetic trees was constructed on RPB2 data. The RPB2 data included sequences from seven Pholiota samples representing four taxa. The best model selected and applied in the Mybayes analysis for the ITS model. BI analysis resulted with an average standard deviation of split frequencies=0.002288. Sequences of the fruit body of HMGIM W140054 had been deposited in GenBank and accession numbers is MN628554, MN628555, MN628556.

[0091] Species, specimens, geographic origin and GenBank accession numbers of sequences used in this study are shown in Table 1.

TABLE-US-00001 TABLE 1 Species, specimens, geographic origin and GenBank accession numbers of sequences used in this study GenBank accession numbers Species name Voucher no. Country ITS LSU RPB2 References Pholiota adiposa CBS 279.29 Netherlands MH855073 MH866533 Vu, D, 2019 (Vu et al., 2019) P. adiposa CBS 561.87 Netherlands MH862102 MH873791 Vu, D, 2019 P. baeosperma TFB8315 USA MF978336 Matheny, P. B, 2018 (Matheny, Swenie, Miller, Petersen, & Hughes, 2018) P. baeosperma TFB7383 USA MF978357 Matheny, P. B, 2018 P highlandensis PBM4085 USA MH360729 Matheny, P. B, 2018 P. highlandensis FIRE409 USA MH360728 Matheny, P. B, 2018 P. lenta MCVE 7100 Italy JF908582 Osmundson, T. W., 2013 (Osmundson et al., 2013) P. lenta CBS 182.53 Netherlands MH857154 MH868690 Vu, D, 2019 P. lenta GLM 45997 Germany AY207270 Walther, G., 2005 (Walther, Garnica, & Weiss, 2005) P. lenta CBS 185.53 Netherlands MH857156 MH868692 Vu, D, 2019 P. limonella G0779 Hungary MK278459 Varga, T, 2019 (Varga et al., 2019) P. limonella KUC20130923-06 Korea KM496470 Jang, S, 2014, Unpublished P. limonella SFC20150707-19 South Korea KX773882 Cho, H. J, 2016 (Cho et al., 2016) P. lubrica PRM 857179 Slovakia HG007984 Holec, J, 2014 (Holec et al., 2014) P. lubrica China JF961353 Tian, E. J, 2011, Unpublished P. lubrica G0252 Hungary MK278460 Varga, T, 2019 P. microspora CBS 360.51 Netherlands MH856901 MH868423 Vu, D, 2019 P. microspora ZJ0005QGG01 China KU836562 Liu, Y, 2016, Unpublished P. multicingulata PDD97861 New Zealand HQ832440 Matheny, P. B., 2010, Unpublished P. glutinosior HMGIM-W140054-1 China MN582988* This study P. glutinosior HMGIM-W140054-2 China MN582989* This study P. glutinosior HMGIM-W140054-3 China MN582990* This study P. glutinosior HMGIM-W140054 China MN582991* This study P. glutinosior HMGIM-W140054-4 China MN628554* This study P. glutinosior HMGIM-W140054-5 China MN628555* This study P. glutinosior HMGIM-W140054-6 China MN628556* This study P. spumosa MCVE3533 Italy JF908577 Osmundson, T. W, 2013 P. spumosa CBS 245.50 France MH856605 Vu, D, 2019 P. spumosa G0436 Hungary MK278465 Varga, T, 2019 P. spumosa Germany AY207272 Walther, G, 2005 (Walther et al., 2005) P. spumosa PBM2849 USA MG923698 Matheny, P. B, 2018 P. spumosa ZRL20151729 China KY419028 Zhao, R. -L, 2017 (Zhao et al., 2017) P. squarrosoides MCVE17140 Finland JF908591 Osmundson, T. W., 2013 P. squarrosoides BHI-F081b USA MF161170 Haelewaters, D, 2018 (Haelewaters, 2018) P. squarrosoides SV.S1 USA AF261641 Moncalvo, J. -M, 2002 (Moncalvo et al., 2002) P. squarrosoides G0510 Hungary MK278467 Varga, T, 2019 Kuehneromyces olrim351 Lithuania AY354218 Lygis, V, 2004 mutabilis (Lygis, Vasiliauskas, & Stenlid, 2004) Kuehneromyces CBS 205.32 Netherlands MH866740 Vu, D, 2019 mutabilis Hypholoma PBM 1844 USA AY337413 Matheny, fasciculare P. B, 2005 *Newly generated sequences for this invention.

[0092] The clone sequencing results of ITS conducted sequenced Blast in GenBank. The six clones were the most similar to Pholiota limonella (registration number: KM496470, From Korea), which is 99.2-99.7%, and has the 98.5-98.7% similarity with Pholiota adiposa (Registration Number: FJ810180, From China). The macroscopic morphology of the strain is not consistent with Pholiota limonella and Pholiota adiposa. The difference is large. The shape of Pholiota adiposa is larger than that of the present invention more than two times, Basidiospores of Pholiota limonella have obvious bud holes, but the species of the invention don't have bud holes, and Basidiospores of this species are smaller than Pholiota limonella. Therefore, it was judged that the strain of the present invention is a new species different from the current species of genus Pholiota.

[0093] The highest similarity of the LSU alignment results was Pholiota squarrosa, which is 98.89% (Registration number: DQ470818, From the United States), apparently not the same strain.

[0094] The RPB2 alignment results have the highest similarity between 86-88% substantially, and are all species of genus Pholiota, which are different from the varieties in the present invention.

[0095] Observing its macroscopic morphology and microscopic morphology: Basidiomata small sized. Pileus (0.65)1.0-2.2 cm in diameter, convex to bell shape when young and the edge of the pileus becoming flat, Orange-yellow, the middle color is darker, no obvious mucus, but it has strong viscous. The middle part of the cap has yellow-brown small spots, and the edge of the cap is wound inside, leaving white fluffy flakes. Stipe. central, 1.14.8 cm, Stick-shaped, fleshy, pale yellow to white, nearly equal in thickness, slightly enlarged and curved at the base, with villiform annulus; the stipe above the annulus is smooth and nearly white, and the stipe below the annulus is light yellow and covered with fluff flakes. Hymenophore. lamella white to off-white color, adnate, sparse, unequal length with thickness is about 0.81.0 mm. Basidiospores. 5.57˜7.11×3.46˜4.38 μm, Q=1.68, long-oval, smooth, with yellowish brown color, partially with oily, bud germ pores are not obvious, non-amyloid. Basidia. 17.8˜25.24×6.8˜7.52 μm, clavate without basal clamp connection, sterigmata up to 23 μm. Cheilocystidia. 16.35˜28.24×7.15˜10.0 μm, long stick shape, smooth without attachments. Pleurocystidia. 18.71˜26.1×6.47˜8.62 μm, short rod to spindle with a short-pointed. Clamp connections were present in tissue of context.

[0096] According to its morphology, the species that is currently consistent with that is not found. The closest one is Pholiota lubrica, but these two are obviously inconsistent in color, Pholiota lubrica is reddish-brown, and the color of the middle and the edge is different. The color is bright yellow and uniform. Moreover, the individuals of Pholiota lubrica are slightly larger, usually distributed in the northeast and the middle of China, and they are far apart from the ITS sequences of these two. Basically, this species is not Pholiota lubrica.

[0097] In order to further confirm its species, we searched the literature for the credible IBS sequence, LSU sequence and RPB2 sequence, using Bayesian method (BI), neighboring method (NJ) and maximum likelihood method (ML), constructed the phylogenetic tree using the Kuehneromyces mutabilis and the clustered Hypholoma fasciculare as the outer group, respectively, as shown in FIG. 4-8.

[0098] From the results of these three phylogenetic trees in FIG. 4 to FIG. 8, the results are consistent. The sequencing results of genus Pholiota are clustered together, indicating that the sequencing results are credible. The highest alignment similarity is Pholiota limonella and Pholiota adiposa for ITS. For LSU, the highest alignment similarity is Pholiota squarrosa. The highest macroscopic and microscopic similarity is Pholiota lubrica. However, in the phylogenetic trees, it cannot be clustered with these species, and the genetic distance is very far. At the same time, its macroscopic and microscopic morphology indicates that it is a species of genus Pholiota, so we judge it as a new species, named Pholiota glutinosior.

Example 2 Artificial Cultivation

[0099] 1. Medium (by Weight Percent):

[0100] (1) Tissue Separation Medium (Integrated Potato Dextrose Agar):

[0101] Potato 20%, glucose 2%, agar 2%, potassium dihydrogen phosphate 0.3%, magnesium sulfate 0.15%, and vitamin B1 trace, the rest is water.

[0102] (2) Purified First-Class Strain Medium (Bengal Red Medium):

[0103] Peptone 0.5%, glucose 1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate (MgSO.sub.4.7H.sub.2O) 0.05%, agar 2%, 1/3000 Bengal red solution 10%, and chloramphenicol 0.01%, and the rest was water.

[0104] (3) Second-Class Strain Culture Medium (Plus Rich Potato Dextrose Agar):

[0105] Potato 20%, glucose 2%, peptone 1%, agar 2%, potassium dihydrogen phosphate 0.3%, magnesium sulfate 0.15%, and vitamin B1 trace, the rest is water.

[0106] (4) Third-Class Strain Culture Medium:

[0107] 98-99% sorghum, and 1-2% calcium carbonate.

[0108] (5) Cultivation Materials:

[0109] 31% cottonseed hulls, 58% wood chips, 10% bran, and 1% CaCO.sub.3; moisture content 60%-65%.

[0110] 2. Method:

[0111] (1) Tissue Isolation Strains:

[0112] The tissue separation medium was prepared, and the medium was dispensed into tube, and sterilized by heat sterilization at 0.11 MPa atmospheric pressure and 121° C. high temperature and high pressure for 30 min, and taken out to cool and be inclined. The collected wild Pholiota fruit body was wiped with 75% alcohol under aseptic conditions, then teared, and 0.2-0.5 mm×0.2-0.5 mm of internal fungus tissue was inoculated into the medium aseptically. Place it in a 25° C. incubator at a constant temperature and dark culture. After the hyphae grows over the slope, it can be transferred. The period is between 10 days and 15 days.

[0113] (2) Production of the Purified First-Class Strain:

[0114] The purified medium was prepared according to the formula, and the medium was dispensed into tube, and sterilized by heat sterilization at 0.11 MPa atmospheric pressure and 121° C. for 30 minutes, and the fungus infected with the bacteria were transferred. It was placed in a 25° C. incubator at a constant temperature for dark culture. When the mycelium grew and the bacteria had not grown, the tip hyphae was picked and transferred to obtain the purified first-class strain.

[0115] (3) Production of the Second-Class Strain:

[0116] The second-class strain culture medium was prepared, and the medium was dispensed into tube, and sterilized by heat sterilization at 0.11 MPa atmospheric pressure and 121° C. for 30 minutes, and cooled down, and then incubated the purified first-class strain which was successfully separated under aseptic operation. Place it in a 25° C. incubator at a constant temperature and dark culture. After the hyphae grows over the slope, the second-class strain can be obtained and then transferred. The period for the second-class strain overgrowing is about 15 days to 20 days.

[0117] (4) Production of the Third-Class Strain

[0118] Weigh the required proportion of sorghum, wet it overnight with water, mix it with calcium carbonate in proportion, put it into 250 ml Erlenmeyer flask with 100-150 g of dry material per bottle, get the third-class strain culture medium, seal with silicone plug, sterilize at 0.147 MPa atmospheric pressure, 128° C. with high temperature, high pressure and humid heat for 90 min, take out and cool down, the medium was shaken and then be inoculated with the second-class strain aseptically. At the time of inoculation, it is ensured that the second-class strain block is buried in the third-class strain stock. Place it in a 25° C. incubator at a constant temperature and dark culture. After the hyphae is full (twenty days or so), it can be used as the third-class strain inoculated in the cultivation bag.

[0119] (5) Cultivation

[0120] The required proportion of culture material by the artificial domestication medium is taken, thoroughly mixed and added with water (water content of 55-65%), and filled into a 17 cm×35 cm high transparent polypropylene cultivation bag with 400-420 g per bag of dry material. After loading the material, make a hole in the bag material using a small wooden stick, and the hole is deep to the bottom of the bag. Then, a plastic ring is placed on the bag mouth, and the matching cover is buckled to obtain a prepared cultivation bag. The mixture was sterilized by heat sterilization at 0.147 MPa atmospheric pressure and 128° C. for 90 minutes. Ensure that the block is buried in the cultivation material during inoculation. After inoculation, the cultivation bags were cultured in the dark at 25° C.±1° C. and air relative humidity of 60-70%. After the hyphae are full (about 25 days), it can enter the cultivation management.

[0121] (6) Cultivation Management (Including after-Ripening Management, Primordium Formation, Fruiting Body Growth)

[0122] {circle around (1)} after-Ripening Management

[0123] After the hyphae in the bag is full of the cultivation material in the bag, the shading is continued for 25 days and then matured for 25 days to enter into the mushrooming stage.

[0124] {circle around (2)} Primordium Formation

[0125] Control the temperature at 18-20° C., and increase the ventilation, keep the space carbon dioxide content below 1%, the air relative humidity is adjusted to more than 90%, after about 14 days, remove the cap and put the cultivation bag vertically (gap between the bags). After about 14 days, the hyphae began to kink and form the pale yellow rice bran-like primordium.

[0126] {circle around (3)} Fruiting Body Growth Period

[0127] After the primordium grows to 0.5 cm, continue to control the temperature at 18-20° C., air relative humidity 80-90%, 9 hours of light per day, light intensity 300-500 lx, and maintain the carbon dioxide concentration in the air 350˜1500 ppm, keep the air moist. It can be harvested after about 8 days. During this period, the water is sprayed 1-2 times a day to the young mushroom until the size of the fruit body is basically unchanged. The fruit body cap no longer grows and begins to flatten, indicating that the fruiting body has matured and should be harvested at this time.

[0128] It takes about 8 days from the growth of the primordial to the maturity of the fruiting body. After harvesting the mushroom firstly, the same mushroom treatment was carried out, and the mushroom was harvested secondly after about 25 days. The results are shown in FIG. 9 and FIG. 10.

[0129] 3. Situation of Mushrooms

[0130] (1) Fruiting period: The first and second mushroom periods total are 90 days, and the first mushroom period is 65 days.

[0131] (2) Yield: The average bag yield is 139.71 grams.

[0132] (3) Fruiting body traits: Basidiomata small sized. Pileus (0.65)1.0-2.2 cm in diameter, convex to bell shape when young and the edge of the pileus becoming flat, Orange-yellow, the middle color is darker, no obvious mucus, but it has strong viscous. The middle part of the cap has yellow-brown small spots, and the edge of the cap is wound inside, leaving white fluffy flakes. Stipe. central, 1.1˜4.8 cm, Stick-shaped, fleshy, pale yellow to white, nearly equal in thickness, slightly enlarged and curved at the base, with villiform annulus; the stipe above the annulus is smooth and nearly white, and the stipe below the annulus is light yellow and covered with fluff flakes. Hymenophore. lamella white to off-white color, adnate, sparse, unequal length with thickness is about 0.8˜1.0 mm.

[0133] Compared with the wild state, the individual fruit body of the species increased significantly after artificial domestication, and the fruiting body was relatively neat and the yield was high.

[0134] After artificial domestication, it can be seen that the traces of charcoal burning adhered to the caps of the mature fruiting bodies, which are significantly different from the species Pholiota glutinosior already described, and it is also confirmed as a new species from the side.

Example 3 Determination of Antibacterial Activity of Fermentation Broth

[0135] 1. Method

[0136] (1) Preparation:

[0137] Bacterial medium: nutrient agar/broth medium;

[0138] Nutrition agar/broth medium: beef paste 3 g, peptone 10 g, sodium chloride 5 g, agar 15 g, add water to 1000 ml, and adjust the pH to 7.4 (nutrient broth without agar).

[0139] Preparation of ethyl acetate extract: using rice medium (formulated rice and water), the strain of the present invention was incubated and cultured for 45 days, ethyl acetate was added, ultrasonic extraction was performed twice, 40 minutes for each time, and the extract was combined. The solvent was recovered in a rotary evaporator, and steamed to about 1-2 mL of the extract, and transferred to a collection bottle, the ethyl acetate extract is obtained and placed in a vacuum desiccator. After the solvent was completely evaporated, it was transferred to a refrigerator at 4° C. for use, and was filtrated and sterilizd with the bacteria syringe and filter for use.

[0140] Positive control preparation: Ampicillin solution, diluted with sterile water to a concentration of 5 μg/mL of the control solution, UV sterilization for 30 min.

[0141] (2) Preparation of Bacteria Suspension

[0142] {circle around (1)} The Staphylococcus aureus cryopreservation solution was taken out from the −80° C. refrigerator, and a single colony was obtained by streaking the plate.

[0143] {circle around (2)}A single colony was picked up with a sterile inoculating loop and inoculated in a nutrient broth medium, and cultured at 37° C., 220 r/min for 24 h, to obtain a bacteria suspension for the test bacteria.

[0144] {circle around (3)} Sucking the bacteria suspension, and diluting with sterile distilled water to obtain the bacteria liquid having a concentration of 10.sup.−1 to 10.sup.−8.

[0145] {circle around (4)} 200 μL of each concentration of the bacteria solution was uniformly applied to the nutrient agar plate, cultured at 37° C. for 24 h, and each concentration was repeated 3 times.

[0146] {circle around (5)} Determine the concentration of the bacteria solution by the plate counting method, and waiting for use.

[0147] (3) Determination of Inhibition Zone

[0148] {circle around (1)} Coating Method

[0149] The counted bacteria solution was diluted to a final concentration of 10.sup.5˜10.sup.6 cfu/mL, and 200 μL of the prepared bacteria suspension was pipetted onto the plate and uniformly coated with a coater. Use a sterilized puncher to evenly punch holes in the bacteria-containing plate, carefully pick out the medium in the well, and pipett the control solution and 2004, of the test sample into the well under aseptic conditions, and place at 37° C. The culture was allowed to stand for 1 day, and the size of inhibition zone was measured.

[0150] {circle around (2)} Pre-Added Bacteria Solution Pouring Plate Method

[0151] Inject a certain amount of bacteria solution into the plate medium that has been cooled to about 50° C., so that the concentration of the bacteria in the medium is 10.sup.5-10.sup.6 cfu/mL, shake well, pour the plate (about 30 mL/plate), and let it stand horizontally to be coagulated. The method of punching and loading is the same as above.

[0152] (4) Data Processing

[0153] Measure and compare the size of inhibition zone.

[0154] 2. Experimental Results:

[0155] The results are shown in FIG. 11 and Table 2.

TABLE-US-00002 TABLE 2 Diaphragm diameter of Staphylococcus aureus (x ± s, n = 3) Sample inhibition zone diameter (mm) Positive control 19.79 ± 1.7  The strain W140054 of the invention 14.17 ± 0.79

[0156] As shown in FIG. 11, the fermentation broth of the strain exhibits an inhibition zone, indicating that the fermentation broth of the present invention has antibacterial activity against Staphylococcus aureus, and about 600 wild edible medicinal bacteria in the same batch are screened. Among the ethyl ester extracts, the strain is one of the top 20 strains with better effects, which indicates that the strain of the present invention has a certain inhibitory effect on Staphylococcus aureus.

Example 4 Determination of Nutrients

[0157] For the artificial cultivation of a new species of genus Pholiota, the nutrient composition was determined, including hydrolysis of amino acids, polysaccharides, proteins and important trace elements. Pholiota adiposa and Pholiota microspora were used as controls, and the results are shown in Table 3.

TABLE-US-00003 TABLE 3 Nutritional ingredients determination table Pholiota Pholiota Pholiota unit glutinosior adiposa microspora Detection method Aspartic acid g/100 g 1.01 1.299 1.360 GB/T *threonine 0.56 0.679 0.811 5009.124-2016 Serine 0.51 0.672 0.810 Glutamate 1.77 3.032 3.383 Glycine 0.50 0.760 0.782 Alanine 0.65 0.908 0.894 *Proline 0.60 0.645 0.683 *methionine 0.11 1.541 1.217 *isoleucine 0.44 0.531 0.631 *Leucine 0.73 0.961 0.926 Tyrosine 0.25 0.284 0.277 *Phenylalanine 0.48 0.751 0.697 Histidine 1.32 0.294 0.318 *lysine 0.63 0.859 0.795 Arginine 0.51 0.802 0.663 Proline 0.51 0.556 0.497 Hydrolyzed 10.6 14.574 14.744 amino acid sum Cystine 0.26 — — HPLC (After oxidative hydrolysis, the sample is determined according to the chromatographic conditions of GB 5009.124-2016.) *Tryptophan 0.21 — — The sample was subjected to alkaline hydrolysis treatment and determined by HPLC. Sum of essential 3.76 5.967 5.760 amino acids Crude polysaccharide g/100 g 7.55 — 3.02 NY/T 1676-2008/7 protein g/100 g 14.9 17.02 18.10 GB/T 5009.5-2016/ First method calcium mg/100 g.sup.  4.01 — 42.1 (NY/ GB/T T1653- 5009.268-2016 2008 Yao Second method Xingyu, 2017) Potassium g/100 g 2730 — 1340 (NY/ GB/T T1653- 5009.268-2016 2008 Yao Second method Xingyu, 2017) magnesium mg/100 g.sup.  73 78.93 90.30 GB/T 5009.268-2016 Second method iron mg/100 g.sup.  3.16 50.77 53.88 GB/T 5009.268-2016 Second method Zinc mg/100 g.sup.  2.77 9.47 7.93 GB/T 5009.268-2016 Second method phosphorus g/100 g 476 — 596 (NY/ GB/T T1653- 5009.268-2016 2008 Yao Second method Xingyu, 2017) Carbohydrate g/100 g 33.5 — — By calculation .sup.3 Crude fiber % 8.1 — 15.5 (Yao GB/T Xingyu, 2017) 5009.10-2003 Total dietary fiber g/100 g 33.0 — — GB 5009.88-2014 fat g/100 g 0.82 4.46 3.16 GB 5009.6-2016/ Second method Moisture g/100 g 10.3 — 9.9 (NY/ GB 5009.3-2016 T1653- First method 2008 Yao Xingyu, 2017) Ash (by sample g/100 g 7.5 8.7 6.6 GB 5009.3-2016 mass) First method Note 1: The data of Pholiota adiposa and Pholiota microspora is from the master's paper of Jilin Agricultural University in 2015: the chemical composition and pharmacological activity of Pholiota adiposa - Wang Xiaoyan. The method for detecting amino acids is: GB/T 5009.124-2003. The rest is the same as the table. Individual missing data refer to the 2017 Kunming University of Science and Technology master's paper: the nutritional composition and chemical composition of Pholiota microspora-Yao Xingyu and has been marked in the form. Note 2: *is essential amino acid. Note 3: Carbohydrate (g/100 g) = 100 − (protein + fat + moisture + ash + dietary fiber)

[0158] From the nutrient composition of the new species of genus Pholiota in Table 3, it has all the essential amino acids and a high histidine content. Histidine is considered to be an essential amino acid for children in the field of nutrition. It can be synthesized in adulthood. It is an essential amino acid for uremic patients and is associated with various allergies and inflammation. Therefore, histidine is also an important class of amino acids. Compared with the nutritional components of the edible and medicinal fungi in “Medicinal and Edible Fungi”, the content of histidine is higher than 99% of the edible and medicinal fungi, and the content is high.

[0159] In addition, it can be seen from Table 3 that the crude polysaccharide content is also much higher than that of Pholiota microspora. Compared with these famous species such as Pholiota adiposa and Pholiota microspora, it is rich in potassium and potassium content is more than 2 times higher than that of Pholiota microspora.

[0160] In summary, the new species of genus Pholiota according to the present invention is a new species that has not been reported yet, and has not been studied yet, and has an effect of inhibiting Staphylococcus aureus, and is rich in nutrient content, and has high cultivation yield. It is a development prospects species with characteristics such as good traits.

[0161] The above is only a preferred embodiment of the present invention, but the scope of the present invention protection is not limited thereto, and for any person skilled in the art within the technical scope disclosed by the present invention, the technology according to the present invention equivalent replacements or modifications of the present invention and its concepts are intended to be included within the scope of the present invention.