Peel plate assembly

Abstract

Peel plates and plate assemblies are shown and described. In one embodiment, a peel plate assembly includes a semi-rigid plate, a removable cover seal, and an adhesive to provide a moisture vapor barrier. In particular embodiments, the moisture vapor barrier provides less than about one pound peel adhesion and a stiffness for reapplication without wrinkling. The result is a peel plate, assembly, and method for the enumerating a microorganism, when present, in a sample.

Claims

1. A peel plate assembly comprising: a. a semi-rigid plate having a recessed well that is sunken below an upper face, wherein a bottom wall of the recessed well on a lower horizontal plane below said upper face, a pair of proximate extensions extending radially and being offset from said lower horizontal plane of said recessed well and independent of said recessed well, and wherein each of said proximate extensions having a tab adjacent said upper face and protruding from said proximate extensions, and at least one raised platform protruding from said upper face and on an upper horizontal plane spaced offset from said lower horizontal plane of said recessed well and independent of said adjacent recessed well; b. a removable cover seal having a perimeter and aligned between said protruding proximate extensions and said protruding raised platform, thereby removably enclosing said recessed well; and c. an adhesive aligned between said pair of proximate extensions and between said removable cover seal and said upper face, and wherein said adhesive provides a moisture vapor barrier along said perimeter around said recessed well in an enclosed position and less than one pound peel adhesion.

2. The peel plate assembly of claim 1, wherein said adhesive comprises a solvent acrylic aligned along a perimeter of said recessed well.

3. The peel plate assembly of claim 2, wherein said adhesive adapted to be removed from said upper face without staining.

4. The peel plate assembly of claim 2, wherein said adhesive adapted to be removed from said upper face without ghosting.

5. The peel plate assembly of claim 1, wherein said assembly comprises about 0.1 to about 0.3 inches of adhesive.

6. The peel plate assembly of claim 5, wherein said assembly comprises about 0.125 inches of adhesive.

7. The peel plate assembly of claim 1, wherein said recessed well includes a grid of a plurality of vertical lines and a plurality of intersecting horizontal lines.

8. The peel plate assembly of claim 1, wherein said recessed well being adapted to receive about five milliliters of sample liquid.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) Embodiments of the disclosure will be better understood by a reading of the Description of Embodiments along with a review of the drawings, in which:

(2) FIG. 1 is a top perspective view of one embodiment of an improved culture peel plate according to the disclosure;

(3) FIG. 2 is a bottom perspective view of the culture plate introduced in FIG. 1;

(4) FIG. 3 is a side perspective view of the culture plate introduced in FIG. 1;

(5) FIG. 4 is one example of a diagnostic procedure utilizing a culture peel plate introduced in FIG. 1:

(6) FIG. 5 is another example of a diagnostic procedure utilizing a culture peel plate introduced in FIG. 1:

(7) FIG. 6 is a further example of a diagnostic procedure utilizing a culture peel plate introduced in FIG. 1:

(8) FIG. 7 is yet another example of a diagnostic procedure utilizing a culture peel plate introduced in FIG. 1:

(9) FIG. 8 is one alternative example of a diagnostic procedure utilizing a culture peel plate introduced in FIG. 1: and

(10) FIG. 9 is a top perspective view of one embodiment of a plurality of stacked culture plates according to the disclosure.

DESCRIPTION OF EMBODIMENTS

(11) In the following description, like reference characters designate like or corresponding parts throughout the several views. Also in the following description, it is to be understood that such terms as forward, rearward, left, right, upwardly, downwardly, and the like are words of convenience and are not to be construed as limiting terms.

(12) FIG. 1 introduces one example of a culture device peel plate 10 for enumerating and/or detecting a microorganism from a sample. The peel plate 10 is a semi-rigid waterproof plate onto which sample may be applied to enumerate microorganisms and the like. As seen in FIG. 1, one example of the peel plate 10 includes a recessed well 12, a distal raised platform 28, and opposing proximate extensions 22 having proximate tabs 18 to support stacked plates as shown and described herein. The upper face 14 of the plate typically has a top periphery 32 around the raised platform. The recessed well 12 includes a sunken wall 24 below the upper face 14. As shown in FIG. 1, the recessed well may include a grid, for instance having vertical line 40 and intersecting horizontal line 42 components useful for colony counting. In particular examples, the grid is on the rear surface. In alternative examples, the grid may be printed in a variety of ways, including inkjet printing, pad printing and the like. Regardless of the printing type, the grid is typically visible through the generally transparent culture device to the front surface and/or rear surface. The plate 10 is also typically transparent material so as to enable observation from the outside, including any of the grids shown and described herein.

(13) FIG. 1 further shows the proximate end of the peel plate 10 includes an access indent 20 with opposing proximate extensions 22 between rounded corners 38. Typically, the proximate extensions 22 include proximate tabs 18 for alignment, including, but not limited to, layering and stacking plates.

(14) FIGS. 2 and 3 show a bottom and side view, respectively, of one example of a peel plate 10 having a raised edge 30 extending above the lower face 16 to define the raised platform 28. Typically, the peel plate has a distal thickness to support any of the elements and testing procedures shown and described herein.

(15) FIG. 4 introduces certain diagnostic steps for enumerating microorganisms, and the like, from a sample. For instance, the peel plate 10 may be placed on a substantially level surface. The peel tab 52 may be lifted concurrently while pressure is applied to the raised platform 28 with the user's fingers, or the like. As next shown in FIG. 5, the tab 52 is lifted vertically upwards and away to expose the culture media 54. In particular the culture media is any of the dried media culture disc shown and described herein. As shown in FIG. 5, the dried media culture disc 54 secured in the recessed well 12 is generally exposed, but the rear portion of the cover remains affixed to a portion of the upper face 14 of the peel plate 10. For instance, the cover remains affixed to the distal portion of the peel plate 10.

(16) FIG. 6 shows one example of dispensing sample 100 to the recessed well. As shown, the peel plate 10 remains on a flat surface and sample 100 is vertically dispensed to the dried media culture disc 54. In particular examples, the sample is expelled from a pipet 102, or similar laboratory tool, rapidly with substantial force and within about two to about three seconds.

(17) FIG. 7 shows one example of the seal and the adhesive forming the removable moisture barrier as shown and described herein. The adhesive is typically aligned between the cover seal and the upper face 14. For instance, the adhesive may be aligned on the cover seal to provide adhesive strength and stiffness, for instance to allow reapplication of the seal about the plate without wrinkling to expose internal elements as understood by those skilled in the art having the benefit of this disclosure.

(18) In certain examples, the adhesive comprises a solvent acrylic to align with corresponding plate elements, for instance along at least a perimeter of recessed well 12. The removable moisture barrier, including but not limited to the adhesive, may remove from the upper face 14 without staining. Similarly, the removable moisture barrier, including but not limited to the adhesive, may remove from the upper face 14 without ghosting. Further, examples include about 0.1 to about 0.3 inches of adhesive, and Applicants have discovered unexpected benefits of a moisture barrier with about 0.125 inches of adhesive.

(19) In some examples, the adhesive bond between the seal and the upper face 14 reduces moisture loss from recessed well 12 to less than about one percent to about three percent during incubation. For instance, the adhesive bond reduces moisture loss to less than about two percent during incubation. In particular examples, the adhesive is a clear, white, transparent, or the like, ultra removable adhesive and provides improved weather ability and ultra violet resistance.

(20) In particular examples, one or more of the edges of the perimeter of the cover seal may include adhesives as shown and described herein to align with corresponding plate surfaces, for instance by pressing around the edges of the plate to ensure a proper seal. In certain examples, wrinkling the cover may be minimized, or eliminated, by slightly pulling the cover forward while re-applying to the plate.

(21) FIG. 8 shows an alternative embodiment for testing filtered water, wherein the sample is filtered through a mixed cellulose filter membrane 200 in recessed well 212. In particular examples, an aspirator under partial vacuum, a syringe, or the like filters the sample. In these examples, the adhesive cover is lifted to pre-wet a filter with sterile water, or the like before reapplying the adhesive cover.

(22) FIG. 9 illustrates one example of layered, or otherwise stacked, peel plates 10a, 10b with aligned proximate tabs 18a, 18b and aligned proximate extensions 22a, 22b as shown and described herein. The stacked arrangement may be incubated as understood by those skilled in the art having the benefit of this disclosure. For instance, the stacked plates may be incubated with their respective adhesive covers 50 down and grid sides aligning upward. Applicants have unexpectantly discovered the plates may be stacked by aligning two pillars and the rectangular platform without affecting plate heat transfer.

(23) General good laboratory practices and precautions should be observed for any of the microbial testing shown and described herein. In certain examples, the peel plate's limit of detection includes one colony forming unit per millimeter (CFU/mL) of sample per filtered sample. Generally, at the end of the diagnostic sequence, including after incubation, the plates may be observed for colony growth, for instance through the bottom side of the plates. In certain examples, one spot may represent one CFU. The sum of the spots may be reported as the CFU/mL or CFH/filtered-water-volume pre sample or sample dilution tested. In particular examples, the filtered samples may be viewed on both the bottom and from the top grid side of the filter, for instance after the adhesive cover is removed. Further, the filter membrane may be viewed under illumination and magnification as understood by those skilled in the art having the benefit of this disclosure to count additional colonies while not reducing TTC or producing other pigments. Still further examples include inserting the peel plate into a reader.

(24) In certain examples, a culture medium mixture may be applied to any of the peel plates shown and described herein The culture medium mixture may include an adhesive; a gelling agent; a bacterial nutritive ingredient; a fibrous material; a percent liquid organic solvent; and a cellulosic material. Typically, wherein upon drying or evaporation of the mixture on the device, the mixture forms a liquid absorbent matrix. The matrix may have a uniform distribution of bacterial growth media that is adapted to absorb and evenly diffuse a liquid sample throughout the absorbent matrix.

(25) In some examples, the gelling agent may be xanthan gum, tica gum, locust bean gum, guar gum, carrageenan, alginate, the like and a combination thereof. The adhesive agent may be hydroxypropylcellulose, hydroxylethylcellulose, solvent soluble cellulosics, the like and a combination thereof. The bacterial nutritive ingredient may comprise EC media. The bacterial nutritive ingredient may comprise plate count agar, plate count agar individual components, the like and a combination thereof. The bacterial nutritive ingredient may comprise yeast and mold growth media. For instance, the bacterial nutritive ingredient may comprise potato dextrose broth.

(26) In particular examples, the bacterial nutritive ingredient may comprise selective medium for growth of indicator organisms. For instance the bacterial nutritive ingredient may comprise growth promoters for bacteria such as coliform, E. coli, enterobacteriaceae, or pathogens. The bacterial nutritive ingredient may comprise growth promoters for pathogens, such as salmonella, listeria, or camphlobacter.

(27) In some examples, the organic solvent comprises ethanol, isopropyl alcohol, the like and a combination thereof. The alcohol percentage may be more than about 50%, including about 60-80%. The organic solvent may contain a percentage of water. The water percentage may be about 1-30%, greater than about 10%, about 15-20% or the like.

(28) The medium may further comprise a growth indicator color-developing agent. In some examples, the color-developing agent includes at least one of the following: triphenyltetrazolium chloride, 3-(p-iodophenyl)-2-(p-nitrophenyl)-5-phenyl-2H-tetrazolium chloride, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, 5-bromo-3-indolyl-beta.-D-galactoside, bromothymol blue, and neutral red. The color-developing agent may include triphenyltetrazolium chloride.

(29) In some examples, the organic solvent comprises a C1-C5 alcohol. In yet other examples, the organic solvent comprises 2-propanol. The medium mixture may include a selective agent. The selective agent may comprise SDS. For instance, the selective agent may comprise bile salts, deoxycholate, sodium citrate or other gram negative selective agents.

(30) Additional medium mixture examples include an enhancer. The enhancer may comprise isopropyl--D-thiogalactoside (IPTG) or another similar analogue to lactose. A color enhancer may comprise x-gal.

(31) In particular examples, the fibrous material comprises Rayon, a Rayon derivative, the like or a combination thereof. The fibrous material may include cellulose acetate. Further, the fibrous material may comprise trilobal Rayon. In certain examples, the fibrous material comprises Rayon and wherein the Rayon has a weight of denier filament in the range of about 0.2 mm to about 1.0 mm. For instance the fibrous material comprises Rayon and wherein the Rayon has a denier per filament of about 0.8 to about 3.0.

(32) The selective bacterial nutritive ingredient may comprise components of EC media. The adhesive may comprise polyvinyl pyrrolidone. In other examples the adhesive comprises polyethylene oxide.

(33) In certain examples, the liquid sample is a liquid extract of solid, a partial solid, and a combination thereof.

(34) Another example of the present disclosure includes a colloidal slurry matrix free of a mesh or weave in any of the peel plates shown and described herein. For instance, in a peel plate device for detecting a microorganism, when present, in a liquid sample, a colloidal slurry matrix free of a mesh or weave may include an adhesive; a gelling agent; a bacterial nutritive ingredient; a plurality of Rayon fiber particles; a percent liquid organic solvent; and cellulosic material. Typically, the matrix is a liquid absorbent matrix having a uniform distribution of bacterial growth media that is generally adapted to absorb and evenly diffuse the liquid sample throughout the matrix.

(35) In certain examples, a culture medium and method for detecting microorganisms in a sample, such as a milk sample, a water sample or other food sample, comprising a mixture that can include an adhesive, a gelling agent, a bacterial nutritive ingredient, a fibrous material, a percent liquid organic solvent, and cellulosic material. Upon addition to a waterproof and flat device and being allowed to dry or evaporate the liquid, the mixture forms a bacterial growth media-infused, liquid absorbent and diffusing, matrix that is adhered to the base of the waterproof and flat device.

(36) The gelling agent can be, for example, one or more of tica gum, xanthan gum, locust bean gum, guar gum, carrageenan, or alginate. The adhesive, when present, can be, for example, one or more of KLUCEL (hydroxypropylcellulose), a solvent soluble cellulosic, for instance carboxymethylcellulose, polyvinyl pyrrolidone and polyethylene oxide. The culture medium bacterial nutritive ingredient can be, for example, EC media or one or more of its individual components thereof or other media and media components known in the art. In other examples, the bacterial nutritive ingredient can be plate count agar or its individual components. The culture medium can also include one or more growth indicator color-developing agents such as triphenyltetrazolium chloride, 3-(p-iodophenyl)-2-(p-nitrophenyl)-5-phenyl-2H-tetrazolium chloride, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, 5-Bromo-4-Chloro-3-Indolyl-B-D-glucuronide; 6-Chloro-3-Indolyl-B-D-galactopyranoside; 5-bromo-3-indolyl-.beta.-D-galactoside, bromothymol blue, and neutral red. The organic solvent can include one or more of ethanol and isopropyl alcohol and can also include, in some aspects, a percentage of water, for example 1-30% water, more than 10% water or in the range of 15-20% water.

(37) In other examples, the solvent can also include one or more alcohols, for example more than 50% or in the range of about 60% to about 80%. Useful alcohols include C1-C5 alcohols such as 2-propanol and ethanol. The waterproof flat plate can be made of, for example, plastic or glass and can be in a typical Petri dish configuration or in a clamshell-like configuration as shown in FIG. 1, and may also include PET plastics, polypropylene, polypropylene and the like. The culture medium can also include an enhancer such as IPTG or another similar analogue to lactose. The fibrous material can include materials such as Rayon or Rayon derivatives or similar materials such as trilobal Rayon, which can be used with a hydrophilic cellulose ester, such as cellulose acetate. The culture medium can also include one or more selective agents such as SDS, antibiotics, deoxycholate, citrate and/or bile salts.

(38) Numerous characteristics and advantages have been set forth in the foregoing description, together with details of structure and function. Many of the novel features are pointed out in the appended claims. The disclosure, however, is illustrative only, and changes may be made in detail, especially in matters of shape, size, and arrangement of parts, within the principle of the disclosure, to the full extent indicated by the broad general meaning of the terms in which the general claims are expressed. It is further noted that, as used in this application, the singular forms a, an, and the include plural referents unless expressly and unequivocally limited to one referent.