Safening Rice Against Group 15 Herbicides

Abstract

The present invention provides methods of reducing injury to rice and achieving weed control by treating the rice with both a safener and a microencapsulated Group 15 herbicide. Herein, the inventors demonstrate that the tolerance of fenclorim-safened rice to the Group 15 herbicide acetochlor is substantially enhanced when this herbicide is applied as a microencapsulated formulation, particularly when it is applied preemergence or delayed preemergence. Rice plants produced by these methods and seeds produced by said rice plants are also provided.

Claims

1. A method of growing rice comprising treating the rice seed with a safener and applying an herbicide to the soil, wherein the herbicide is a Group 15 herbicide, and wherein the herbicide is microencapsulated.

2. The method of claim 1, wherein the herbicide is selected from the group consisting of acetochlor, metolachlor, dimethenamid, and alachlor.

3. The method of claim 1, wherein the herbicide is applied prior to or after the rice is planted.

4. The method of claim 3, wherein the herbicide application is preemergence or delayed preemergence.

5. The method of claim 1, wherein the herbicide is applied at a rate of at least 250 grams active ingredient per hectare.

6. The method claim 5, wherein the herbicide is applied at a rate of at least 1,260 grams active ingredient per hectare.

7. The method of claim 1, wherein the safener is fenclorim.

8. The method of claim 1, wherein the safener is applied to the rice seeds prior to planting.

9. The method of claim 8, wherein the rice seed is coated with the safener prior to planting.

10. The method of claim 1, wherein the rice is Oryza sativa L.

11. The method of claim 1, wherein weeds are controlled by the herbicide.

12. The method of claim 11, wherein the herbicide controls barnyardgrass and/or weedy rice.

13. The method of claim 11, wherein the rate of weed control is at least 50%.

14. The method of claim 1, wherein the method leads to reduced crop injury and/or reduced stand reduction caused by the herbicide as compared to crops treated with the herbicide alone.

15. The method of claim 14, wherein the rate of crop injury is no more than 20% and/or the rate of stand reduction is no more than 20%.

16. The method of claim 1, wherein a commercially acceptable rate of weed control and a commercially acceptable rate of crop injury are simultaneously attained.

17. The method of claim 1, wherein the herbicide is acetochlor, wherein the safener is fenclorim, and wherein the safener is applied to the rice seeds prior to planting.

18. The method of claim 1, wherein the rice is grown in cool and moist environmental conditions.

19. A rice plant produced by the method of claim 1.

20. A seed produced by the rice plant of claim 19.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0009] FIG. 1 is a set of photographs of a greenhouse experiment comparing weedy rice and cultivated rice to which 1,050 grams active ingredient per hectare of microencapsulated (ME) acetochlor (Warrant, Bayer CropScience, St. Louis, Mo.) was applied at the same time the seeds were planted with cultivated rice alone or cultivated rice seeds treated with the safener provided herein when the acetochlor was added to the soil three weeks prior to planting. A decreased amount of injury was observed when the herbicide was applied prior to planting and also when the seeds were pre-treated with safener.

[0010] FIG. 2 is a bar graph showing the results of the greenhouse experiment of FIG. 1 as a percentage of injury to the plants.

[0011] FIG. 3 is a bar graph showing the percent rice injury 21 days after treatment (DAT) with a combination of the safener fenclorim and the herbicide ME acetochlor, applied preemergence. Acetochlor, formulated as an emulsifiable concentrate (EC; dark gray bars) or microencapsulatated (ME; light gray bars), was applied at rates of 313, 632, 1,262 and 2,524 grams active ingredient per hectare.

[0012] FIG. 4 is a bar graph showing the percent rice injury 21 days after treatment (DAT) with a combination of the safener fenclorim and the herbicide acetochlor, applied delayed preemergence four days after planting. Acetochlor, formulated as an emulsifiable concentrate (EC; dark gray bars) or microencapsulatated (ME; light gray bars), was applied at rates of 313, 632, and 1,262 grams active ingredient per hectare.

[0013] FIG. 5 is a set of photographs of acetochlor-treated rice (left), nontreated rice (middle), and rice that were treated with a fenclorim seed treatment followed by acetochlor (right) four weeks after application. The ME acetochlor was applied delayed-preemergence.

DETAILED DESCRIPTION

[0014] In accordance with the present invention, it has been discovered that treating rice with both a safener and a microencapsulated Group 15 herbicide reduces rice injury to commercially acceptable levels while still providing effective weedy rice control. In the Examples, rice is treated with a combination of the safener fenclorim and a microencapsulated formulation of the Group 15 herbicide acetochlor. Importantly, the inventors show that application of either fenclorim or microencapsulated acetochlor alone provides only partial protection for rice. Thus, combination of these two treatments is required to achieve adequate tolerance to commercial use rates of Group 15 herbicides.

[0015] The present invention provides methods of growing rice, which involve treating the rice with a combination of a safener and a microencapsulated Group 15 herbicide.

Herbicides

[0016] As used herein, the term herbicide is used to refer to a substance used to destroy or inhibit unwanted vegetation. Where an herbicide is referenced generically herein by name, unless otherwise restricted, that herbicide includes all commercially available forms known in the art such as salts, esters, free acids and free bases, as well as stereoisomers thereof. Herbicides are commonly used as emulsifiable concentrates (EC). However, in the present invention, the principal herbicide is also utilized in a microencapsulated (ME) formulation in which herbicide molecules are protected from degradation processes by a porous, polymer shell. See for example U.S. Pat. No. 9,877478, which is incorporated herein by reference in its entirety. When exposed to soil moisture (e.g., by an activating rainfall), the polymer shell dissolves and allows a slow release of herbicide. The delayed release of herbicide allows time for rice to imbibe soil water during germination and grow uninhibited for a period following application. In addition, a gradual release of herbicide over time allows the herbicide to provide longer residual control of targeted weeds relative to the EC formulation. In the present application and elsewhere, the inventors have demonstrated that rice is more tolerant to ME herbicide formulations than to EC, likely due to the potential for immediate absorption of herbicide from the EC formulation following rainfall (Weed Technology (2018) 33:239-245).

[0017] In microencapsulated formulations, the release rate of the core material can be controlled through selection of several parameters including: the shell wall composition, the weight ratio of the herbicide to shell wall material, the core material components, the mean microcapsule particle size, process conditions such as mixing shear and time, and combinations thereof. In some formulations, a diluent, such as a solvent, may be added to change the solubility characteristics of the core material as to increase or decrease the release rate of the active ingredient from the microcapsule. The diluent may be selected from essentially any of those known in the art, as long as it is compatible with the core material and shell. In some formulations, the core material may comprise a blend of a first and second population of a particulate microencapsulated herbicide provide a multi-modal (e.g., bimodal) release rate. Additional ingredients may be added to the core material to improve its properties. Exemplary ingredients include, without limitation, thickeners, stabilizers, anti-packing agents, drift control agents, biocides or preservatives, antifreeze agents, and antifoam agents.

[0018] Group 15 herbicides are very-long-chain fatty acid (VLCFA)-inhibitors. Accordingly, the terms Group 15 herbicide, VLCA-inhibitor, and VLCFA-inhibiting herbicide are used interchangeably throughout the present application. These soil-applied herbicides are primarily absorbed through seedling shoots and roots where they inhibit cell development and cell division. Only five weed species worldwide are resistant to VLCFA-inhibiting herbicides, which suggests there is a lower risk for resistance to this class of herbicides relative to other rice herbicides. VLCFA-inhibiting herbicides include the chloroacetanilide chemical family (e.g., acetochlor, alachlor, metolachlor, dimethenamid, pethoxamid, pretilachlor, butachlor) and the isoxazoline chemical family (e.g., pyroxasulfone).

[0019] While the VLCFA-inhibitors pretilachlor and butachlor are commonly used in Asian rice production, they provide only marginal weed control. Thus, in preferred embodiments, the herbicide used in the present invention provides weed control at commercially acceptable levels. Suitable herbicides include, without limitation, acetolachlor, metolachlor and dimethenamid. In particularly preferred embodiments, the herbicide acetochlor is utilized. Acetochlor is a widely used VLCFA-inhibitor belonging to the chloroacetamide family. Currently, in the United States, it is labeled for use in corn, cotton, soybean, and grain sorghum. Acetochlor is generally applied preemergence for control of annual grasses and small-seeded broadleaves. Acetochlor is commercially available in a microencapsulated (ME) formulation (Warrant, Monsanto Company, St. Louis, Mo.).

[0020] In the methods of the present invention, the Group 15 herbicide may be applied either prior to or after the rice is planted. In some embodiments, the herbicide is applied to a field 1-250 days prior to planting. In other embodiments, the herbicide is applied after planting, and the application may be either preemergence or postemergence. Compared to preemergent plants, emergent plants are typically relatively unaffected by seedling growth inhibitor herbicides. For that reason, prior art practice has been to apply herbicides after crop emergence, but before weed emergence. Postemergence treatments include early postemergence (EPOST) application, as well as application at the spiking, one- to two-leaf, or three- to four-leaf stage. In some embodiments, the herbicide application is preemergence. Preemergence refers to anytime during the interval from the planting of a crop plant up to, but not including, emergence of the crop plant (i.e., before cracking or spiking). Preemergence treatment includes both the treatment of the crop area before sowing (i.e., preplant incorporation), and the treatment of the sown crop areas in which the plants have not yet emerged. Notably, the activity of herbicides such as acetochlor is highly dependent upon rainfall, and greater potential for rice injury exists at earlier application timings. Thus, in other embodiments the Group 15 herbicide is applied delayed preemergence. Delayed preemergence generally refers to the time at least 4 days after planting and as many as 14 days after planting, when seed has imbibed moisture and sprouted but prior to emergence of the seedlings.

[0021] The effective amount of microcapsules to be applied to an agricultural field is dependent upon the identity of the encapsulated herbicide, the release rate of the microcapsules, the crop to be treated, and environmental conditions, especially soil type and moisture. Generally, application rates of herbicides, such as, for example, acetochlor, are on the order of about 0.1, 0.2, 0.5, 1, 2, 3, 4 or 5 kilograms of herbicide per hectare, or ranges thereof, such as from 0.1 to 5 kilograms per hectare, 0.2 to 4 kilograms per hectare, from 0.25 to 2 kilograms per hectare, or from 0.5 to 1 kilograms per hectare. Suitably, an application rate from about 0.25 to about 2 kilograms per hectare is used. In some embodiments, the herbicide is applied at a rate of at least 250 grams active ingredient per hectare. In other embodiments, the herbicide is applied at a rate of at least 1,260 grams active ingredient per hectare.

[0022] The methods may be applied in combination with additional herbicides. Applying several herbicides with distinct mechanisms of action may be useful, for example, for treating fields with herbicide-resistant weeds such as barnyardgrass. Exemplary co-herbicides include, without limitation, ACCase inhibitors (e.g., aryloxyphenoxypropionics), enolpyruvyl shikimate-3-phosphate synthaste (EPSPS) inhibitors (e.g., glyphosate), glutamine synthetase inhibitors (e.g., glufosinate), synthetic auxins (e.g., aromatic acid, phenoxy and pyridine herbicides), photosystem II (PS II) inhibitors (e.g., ureas and triazines), ALS or AHAS inhibitors (e.g., sulfonylureas, triazolopyrimidines and imidazolinones), photosystem I (PS I) inhibitors (e.g., paraquat), protoporphyrinogen oxidase (PPO) inhibitors (e.g., diphenyl ethers, phenyl pyrazoles, aryl triazones and oxadiazoles), mitosis inhibitors (e.g., anilide, amide, certain organophosphorus and carbanilate herbicides), cellulose inhibitors (e.g., nitrile and oxazole herbicides), oxidative phosphorylation uncouplers, dihydropteroate synthase inhibitors, fatty acid and lipid biosynthesis inhibitors (e.g., thiocarbamate and certain organophosphorus herbicides), auxin transport inhibitors (e.g., amide and urea herbicides) and carotenoid biosynthesis inhibitors (e.g., isoxazolidinone, benzoylcyclohexanedione and benzoylpyrazole herbicides), salts and esters thereof, and mixtures thereof. In some embodiments of the present invention, the one or more co-herbicides are not encapsulated.

Safeners

[0023] As used herein, the word safener is used to refer to compounds that antagonize the harmful effects of an herbicide on cultivated plants. Safeners were formerly referred to as antidotes, and these terms may be used interchangeably. Preferably, these compounds protect cultivated plants without noticeably influencing the herbicide's action on the weeds that it is meant to control. The methods of the present invention may utilize any safener that protects rice plants against injury from a Group 15 herbicide. In preferred embodiments, the safener is fenclorim (4,6-dichloro-2-phenyl-pyrimidine).

[0024] Safeners are most effective when applied prior to or simultaneously with the herbicides whose injury they prevent. Depending on their properties, safeners can be used for pretreating the seeds of the cultivated plants (dressing seeds or seedlings), can be incorporated in the soil (e.g., in the furrow) before or after sowing seeds, or else applied alone or together with the herbicide (e.g., as a tank mixture) before or after emergence of the plants. Thus, treatment of the plant or seeds with the safener can be carried out independently of the time of application of the herbicide, or alternatively, the treatment can be carried out simultaneously. In preferred embodiments, the safener is applied to the seeds prior to planting (i.e., by coating the seeds with the safener).

[0025] The rate of application in which the safener is applied in relation to the herbicide depend largely on the mode of application. When the safener is applied as a field treatment, either alone or as a tank mixture with the herbicide, the ratio of safener to herbicide is usually from 1:100 to 10:1, but more typically from 1:5 to 8:1. However, when safeners are applied as a seed dressing, much smaller amounts per hectare of crop area are required than when they are applied later. For seed dressing, 0.1 to 10 g of safener is usually required per kg of seeds, with the preferred amount being from 0.1 to 3 g per kg seed.

[0026] The safener may be used in an unmodified form or as a composition with conventional adjuvants and carriers. Safeners may be formulated in any known manner, for example, as emulsifiable concentrates, directly sprayable or dilutable solutions, dilute emulsions, wettable powders, soluble powders, dusts, granulates, and also encapsulations in e.g. polymer substances. The compositions can also contain further ingredients such as stabilizers, antifoams, viscosity regulators, binders, adhesives, as well as fertilizers or other active compounds, in order to attain special effects.

[0027] The safener formulations are prepared in known manner, e.g. by mixing and/or grinding the active ingredients with extenders, e.g. solvents, solid carriers and, where appropriate, surface-active compounds (surfactants). Suitable safener solvents include without limitation: aromatic hydrocarbons, preferably the fractions containing 8 to 12 carbon atoms, e.g. xylene mixtures or substituted naphthalenes, phthalates such as dibutyl phthalate or dioctyl phthalate, aliphatic hydrocarbons such as cyclohexane, or paraffins, alcohols and glycols and their ethers and esters, such as ethanol, ethylene glycol, ethylene glycol monomethyl or monoethyl ether; ketones such as cyclohexanone, strongly polar solvents such as N-methyl-2-pyrrolidone, dimethyl sulfoxide or dimethyl formamide; as well as epoxidised vegetable oils such as epoxidised coconut oil or soybean oil; or water. Suitable solid carriers used e.g. for dusts and dispersible powders are normally natural mineral fillers such as calcite, talcum, kaolin, montmorillonite or attapulgite. In order to improve the physical properties it is also possible to add highly dispersed silicic acid or highly dispersed absorbent polymers. Suitable granulated adsorptive carriers are porous types, for example pumice, broken brick, sepiolite or bentonite; and suitable nonsorbent carriers are materials such as calcite or sand. In addition, a great number of pregranulated materials of inorganic or organic nature can be used, e.g. especially dolomite or pulverized plant residues. Depending on the nature of the safener to be formulated, suitable surface-active compounds are nonionic, cationic and/or anionic surfactants having good emulsifying, dispersing and wetting properties. The surfactants customarily employed in such formulations are described e.g. in the following publications: McCutcheon's Detergents and Emulsifiers Annual, MC Publishing Corp., Ringwood, N.J., 1979; Sisely and Wood, Encyclopedia of Surface Active Agents, Chemical Publishing Co. Inc., New York, 1964.

[0028] At the molecular level, herbicides and their respective safeners are usually quite similar. Thus, safeners may act either as bioregulators that influence the amount of an herbicide that reaches its target site in an active form or as antagonists of herbicides at a similar site of action. While some safeners reduce the amount of herbicide reaching its site of action by reducing the rate of its uptake and/or translocation, most currently developed safeners function by enhancing the rate of metabolic detoxification. Safeners belonging to several chemical classes (i.e., phenylpyrimidines, dichloroacetamides, oxime ethers, and thiazoles) are thought to protect plants against injury from chloroacetanilide herbicides (one class of Group 15 herbicides) by enhancing the conjugation of these herbicides to the reduced form of the thiol glutathione. After an herbicide is conjugated with glutathione in the cytoplasm, it is sequestered in a vacuole for degradation, resulting in its detoxification. Glutathione S-transferase enzymes (GSTs) catalyze conjugation of glutathione to various substrates. Thus, these safeners may function either by elevating the levels of reduced glutathione or by inducing the activity of GSTs.

Application

[0029] The methods of the present invention may be used to grow any cultivated rice variety. Cultivated rice is typically from the genus Oryza, and most commonly from the species Oryza sativa and Oryza glaberrima. The rice utilized in the present invention may include both non-traited rice and traited rice varieties. As used herein, the term traited rice is used to refer to rice with an herbicide resistance trait. Commonly used traited rice varieties include imidazolinone-resistant (Clearfield, BASF Corporation, Research Triangle Park, N.C.) rice and quizalofop-resistant (Provisia, BASF Corporation, Research Triangle Park, N.C.) rice. While these traited varieties are exceptionally popular, extensive use of certain inhibitors used with these varieties and poor adherence to stewardship guidelines has quickly led to the development of resistance among several weed populations. Thus, the methods of the present invention, which utilize an herbicide with a distinct site of action, are especially useful for growing traited varieties plagued by resistant weeds. Specific cultivars that may be used with the methods provided herein include, but are not limited to, rice cultivar Diamond, Titan, FP 7521, FP 7321, PVL01, and PVL02.

[0030] The rice treated with the methods of the present invention may be planted using several techniques. In the United States, rice production is broadly categorized as either dry-seeded or water-seeded. In the dry-seeded system, rice is sown into a prepared seedbed with a grain drill or by broadcasting the seed and incorporating it with a disk or harrow. Moisture for seed germination is then provided by irrigation or rainfall. In preferred embodiments, the safener is applied to the rice seeds as a dusting or concentrated formulation, and the seeds are planted by a dry-seeded method. In contrast, in water-seeded systems, rice seed is soaked for 12 to 36 hours to initiate germination, and the seed is broadcast by airplane into a flooded field. The seedlings emerge through a shallow flood, or the water may be drained from the field for a short period of time to enhance seedling establishment. Thus, in embodiments in which a water-seeded system are utilized, the safener may be applied in the soaking solution used to initiate germination.

[0031] Further, the rice treated with the methods of the present invention may be grown in unfavorable environmental conditions. Specifically, the inventors have demonstrated that the methods may be used in cool, moist conditions (see Example 5). Thus, in some embodiments, the rice is grown in cool and moist environmental conditions. Temperatures ranging from 10-15 C. can result in cold damage to rice plants, depending on the reproductive stage of the plant. Thus, a climate with night temperatures that average between 10 C. and 16 C. and/or day temperatures that average between 20 C. and 27 C. may be considered cool for growing rice. As used herein, moist conditions for growing rice include those in which soil moisture averages 70%-90%.

[0032] The experiment was conducted within a growth chamber at 13.8 C. (night) and 23.8 C. (day) using a 10-hour night and 14-hour day photoperiod. Diamond rice was planted in pots filled to the same weight of soil to maintain 80% soil moisture throughout the experiment. Treatments consisted of an application of ME acetochlor at 0 and 1,050 g ai ha.sup.1 and a fenclorim seed treatment of 0 and 2.5 g ai kg.sup.1 seed. Data were analyzed using R and subjected to ANOVA. Means were separated using Fisher's LSD (=0.05).

[0033] The methods of the present invention may be useful for controlling a wide variety of weeds, i.e., plants that are considered a nuisance or a competitor of commercially important crop plants. Examples of weeds that may be controlled according to the method of the present invention include, but are not limited to, barnyardgrass (Echinochloa crus-galli) and other weed species within the Echinochloa genus, crabgrasses within the genus Digitaria, Palmer amaranth (Amaranthus palmeri) and other weed species within the Amaranthus genus, common purslane (Portulaca oleracea) and other weed species in the Portulaca genus, Chenopodium album and other Chenopodium spp., Setaria lutescens and other Setaria spp., Solanum nigrum and other Solanum spp., Lolium multiflorum and other Lolium spp., Brachiaria platyphylla and other Brachiaria spp., Conyza canadensis and other Conyza spp., and Eleusine indica. In certain preferred embodiments, the weed species is Oryza sativa L. var. sylvatica (weedy rice) or Oryza sativa L. (red rice).

[0034] As further used herein, weed control refers to any observable measure of control of plant growth, which can include one or more of the actions of (1) killing, (2) inhibiting growth, reproduction or proliferation, and (3) removing, destroying, or otherwise diminishing the occurrence and activity of plants. Weed control can be measured by any of the various methods known in the art. For example, weed control can be determined as a percentage as compared to untreated plants following a standard procedure wherein plant mortality and growth reduction is visually assessed by one skilled in the art. Control may be defined, for instance, in terms of mean plant weight reduction or percentage of plants that fail to emerge following preemergence herbicide application. A commercially acceptable rate of weed control varies with the weed species, degree of infestation, environmental conditions, and the associated crop plant. Commercially effective weed control may be defined as the destruction (or inhibition) of at least about 50%, 55%, 60%, 65%, 70%, 75%, 80%, or even at least 85%, or even at least 90%. Although it is generally preferable from a commercial viewpoint that 80% or more of the weeds be destroyed, commercially acceptable weed control can occur at much lower destruction or inhibition levels, particularly with some very noxious, herbicide-resistant plants. Advantageously, the herbicidal microcapsules used in accordance with the present invention achieve commercially acceptable weed control in the time period of from application to 3 weeks, 4 weeks, 5 weeks, 6 weeks, 7 weeks, 8 weeks, 9 weeks, 10 weeks, 11 weeks, or even 12 weeks after application of the herbicide microcapsules.

[0035] Crop injury can be measured by any means known in the art, such as those described above for weed control determination. A commercially acceptable rate of crop injury for the present invention likewise varies with the crop plant species. Typically, a commercially acceptable rate of crop injury is defined less than about 20%, 18%, 16%, 15%, 13%, 12%, 11%, 10% or even less than about 5%. The methods of the present invention limit crop injury to a commercially acceptable rate as measured from about 24 hours (about 1 DAT) after application to three weeks (about 21 DAT).

[0036] The present disclosure is not limited to the specific details of construction, arrangement of components, or method steps set forth herein. The compositions and methods disclosed herein are capable of being made, practiced, used, carried out and/or formed in various ways that will be apparent to one of skill in the art in light of the disclosure that follows. The phraseology and terminology used herein is for the purpose of description only and should not be regarded as limiting to the scope of the claims. Ordinal indicators, such as first, second, and third, as used in the description and the claims to refer to various structures or method steps, are not meant to be construed to indicate any specific structures or steps, or any particular order or configuration to such structures or steps. All methods described herein can be performed in any suitable order unless otherwise indicated herein or otherwise clearly contradicted by context. The use of any and all examples, or exemplary language (e.g., such as) provided herein, is intended merely to facilitate the disclosure and does not imply any limitation on the scope of the disclosure unless otherwise claimed. No language in the specification, and no structures shown in the drawings, should be construed as indicating that any non-claimed element is essential to the practice of the disclosed subject matter. The use herein of the terms including, comprising, or having, and variations thereof, is meant to encompass the elements listed thereafter and equivalents thereof, as well as additional elements. Embodiments recited as including, comprising, or having certain elements are also contemplated as consisting essentially of and consisting of those certain elements.

[0037] Recitation of ranges of values herein are merely intended to serve as a shorthand method of referring individually to each separate value falling within the range, unless otherwise indicated herein, and each separate value is incorporated into the specification as if it were individually recited herein. For example, if a concentration range is stated as 1% to 50%, it is intended that values such as 2% to 40%, 10% to 30%, or 1% to 3%, etc., are expressly enumerated in this specification. These are only examples of what is specifically intended, and all possible combinations of numerical values between and including the lowest value and the highest value enumerated are to be considered to be expressly stated in this disclosure. Use of the word about to describe a particular recited amount or range of amounts is meant to indicate that values very near to the recited amount are included in that amount, such as values that could or naturally would be accounted for due to manufacturing tolerances, instrument and human error in forming measurements, and the like. All percentages referring to amounts are by weight unless indicated otherwise.

[0038] No admission is made that any reference, including any non-patent or patent document cited in this specification, constitutes prior art. In particular, it will be understood that, unless otherwise stated, reference to any document herein does not constitute an admission that any of these documents forms part of the common general knowledge in the art in the United States or in any other country. Any discussion of the references states what their authors assert, and the applicant reserves the right to challenge the accuracy and pertinence of any of the documents cited herein. All references cited herein are fully incorporated by reference, unless explicitly indicated otherwise. The present disclosure shall control in the event there are any disparities between any definitions and/or description found in the cited references.

[0039] The following examples are meant only to be illustrative and are not meant as limitations on the scope of the invention or of the appended claims. The present invention also provides rice plants grown by the methods disclosed herein, as well as seeds produced by these rice plants.

EXAMPLES

[0040] In previous studies, we determined rice tolerance to the Group 15 herbicide acetochlor is improved by providing this herbicide in a microencapsulated (ME), slow-release formulation (Weed Technology (2018) 33:239-245). In the following Example, rice was treated with a combination of a ME acetochlor and the safener fenclorim. Fenclorim was applied as a seed treatment, and ME acetochlor was applied either preemergence or delayed preemergence.

Example 1

Greenhouse Study

[0041] To determine if timing of herbicide application or any other process would enable the use of Group 15 herbicides on rice, we performed several greenhouse experiments. FIG. 1 is a set of photographs of a greenhouse experiment demonstrating that the Group 15 herbicide acetochlor was able to effectively reduce weedy rice growth when applied at planting. However, when microencapsulated (ME) acetochlor was applied to rice crops at planting, it caused a great deal of damage to the rice (over 80% injury), as reported in FIG. 2. In contrast, when acetochlor was applied three weeks prior to planting, the injury to the rice crop was reduced to about 50%, which is still too much injury for commercial use. Finally, cultivated rice seeds were treated with the safener fenclorim and the ME acetochlor herbicide was applied to the soil three weeks prior to planting. In this experiment, the injury to the rice was only about 10% (see FIG. 2), which would be acceptable for commercial use.

Example 2

Field Study

Materials and Methods:

[0042] Field experiments were conducted to assess the tolerance of fenclorim-safened rice to different rates, timings, and formulations of acetochlor. All experiments were conducted in a silt loam soil. Prior to experimentation, seeds of the rice cultivar, Diamond, were coated with fenclorim at 0, 0.25, or 2.5 grams per kilogram of seed (g kg seed.sup.1) using standard procedures. Experiments were setup using a split-plot design with acetochlor rate and formulation as the main plot and fenclorim treated seeds as the subplot. Treatments were replicated four times. Each plot was 2.2 m wide by 1.5 m long and surrounded by a 1.5 m border. In each plot, rice seeds were hand planted at 22 seeds per 0.3 m in furrows spaced 15 cm apart created by a 10-row cone drill planter. Seeds treated with fenclorim at 0, 0.25, and 2.5 g kg seed.sup.1 were planted into rows 2 and 3, 5 and 6, and 8 and 9, respectively.

Results:

Treatment 1: Acetochlor Applied Preemergence

[0043] Acetochlor formulated as an emulsifiable concentrate (EC, Harness) or microencapsulated (ME, Warrant) was applied preemergence at the equivalent acetochlor rates of 313, 632, 1,262 and 2,524 grams active ingredient per hectare (g ai ha.sup.1). Applications were made using a CO.sub.2-pressurized backpack sprayer fitted with a 110015 AIXR nozzles calibrated to deliver 140 liters per hectare (L ha.sup.1) at 4.8 kilometers per hour (km hr.sup.1). Afterwards, plots were irrigated using an overhead lateral irrigation system to incorporate herbicides into the soil solution. At 21 days post-treatment, rice injury was recorded and the results are presented in FIG. 3.

Treatment 2: Acetrochlor Applied Delayed Preemergence

[0044] Acetochlor formulated as an emulsifiable concentrate (EC, Harness) or microencapsulatated (ME, Warrant) was applied delayed preemergence at the equivalent acetochlor rates of 313, 632, and 1,262 g ai ha.sup.1. Applications were made using a CO.sub.2-pressurized backpack sprayer fitted with a 110015 AIXR nozzles calibrated to deliver 140 L ha.sup.1 at 4.8 km hr.sup.1. After rice seeds were planted, the plots were irrigated overhead with a lateral irrigation system to promote rice germination. Herbicide treatments were applied four days later, and herbicides were incorporated into soil solution by a natural rain event two days later. The coleoptile of rice plants was beginning to protrude from the soil at this time (spiking stage). At 21 days post-treatment, rice injury was recorded and the results are presented in FIG. 4.

Conclusions:

[0045] Rice treated with a combination of a microencapsulated acetochlor and the safener fenclorim displayed increased herbicide tolerance, enabling the robust Group 15 herbicide acetochlor to be used to control grass weeds such as weedy rice. While this combination treatment is effective both when the acetochlor is applied preemergence and when it is applied delayed preemergence, the most dramatic reduction in rice injury was observed with the delayed application.

Example 3

Tolerance of Rice to Preemergence (PRE) and Delayed-Preemergence (DPRE) Applications of Microencapsulated (ME) Acetochlor

[0046] An experiment was conducted in the Spring of 2020 to determine if a fenclorim rice seed treatment provides enhanced rice crop tolerance to microencapsulated (ME) acetochlor under typical planting conditions. The rice cultivar Diamond was planted at 72 seeds m.sup.1 row. Rice plots were treated using standard small-plot research application practices. Treatments consisted of two application timings [preemergence (PRE) and delayed-preemergence (DPRE)], three rates of ME acetochlor (0, 1,260, and 1,890 g ai ha.sup.1), and a fenclorim seed treatment (0 and 2.5 g ai kg.sup.1 seed). Data were analyzed using R, subjected to analysis of variance (ANOVA), and means were separated using Fisher's LSD (=0.05).

TABLE-US-00001 TABLE 1 Stand reduction of Diamond rice caused by ME acetochlor relative to the nontreated check averaged over application timing and rate three weeks after treatment. Fenclorim SDTR.sup.a rate (g ai kg.sup.1 seed) Stand reduction (%) 0 30 a.sup.b 2.5 13 b .sup.aSeed treatment .sup.bDifferent letters within a column indicate a significant difference between treatments; means separated using Fisher's LSD at = 0.05

TABLE-US-00002 TABLE 2 Stand reduction of Diamond rice relative to the nontreated check 3 weeks after treatment. Application Acetochlor rate Fenclorim Stand timing (g ai ha.sup.1) SDTR.sup.a reduction (%) PRE 1,260 0 23 BC.sup.b 2.5 13 D 1,890 0 33 A 2.5 16 CD DPRE 1,260 0 34 A 2.5 13 D 1,890 0 28 AB 2.5 10 D .sup.aSeed treatment g ai kg.sup.1 seed .sup.bMeans within a column followed by the same letter are not significantly different based on Fisher's LSD at = 0.05

Conclusions:

[0047] The fenclorim seed treatment demonstrated safening effects in the form of reduced stand loss from ME acetochlor. Averaged over application timing and acetochlor rate, stand loss from acetochlor was reduced by greater than 50% (Table 1). Though insignificant, when comparing treatments with PRE and DPRE application timings as well as acetochlor rates, stand reduction was greater in plots without the seed treatment of fenclorim (Table 2). Stand reduction was no more than 16% for treatments including the fenclorim seed treatment with the PRE application timing and no more than 13% with the DPRE application timing.

Example 4

Tolerance of Multiple Rice Cultivars to ME Acetochlor Using a Fenclorim Seed Treatment.

[0048] An experiment was conducted in the Spring of 2020 to determine cultivar response to a fenclorim rice seed treatment and ME acetochlor. Two inbred rice cultivars (Diamond and Titan) and two hybrid cultivars (FP 7521 and FP 7321) were planted at 72 and 36 seeds m.sup.1 row, respectively. Rice plots were treated using standard small-plot research application practices. Treatments consisted of an application of ME acetochlor at 0 and 1,260 g ai ha.sup.1 and a fenclorim seed treatment of 0, 2.5, and 5.0 g ai kg.sup.1 seed. Cultivars were analyzed separately using R and subjected to ANOVA, and means were separated using Fisher's LSD (=0.05).

TABLE-US-00003 TABLE 3 Visual rice groundcover of four cultivars in response to ME acetochlor rate six weeks after crop emergence. ME Groundcover (%) acetochlor.sup.a SDTR.sup.b Diamond Titan FP 7521 FP 7321 0 0 87 A.sup.c 81 AB 97 A 74 A 2.5 80 AB 91 A 96 A 96 A 5.0 81 AB 96 A 93 AB 94 A 1,260 0 69 C 59 B 75 BC 74 A 2.5 73 BC 89 A 74 C 86 A 5.0 81 AB 89 A 85 ABC 73 A .sup.ag ai ha.sup.1 .sup.bFenclorim seed treatment (g ai kg.sup.1 seed) .sup.cMeans within a column followed by the same letter are not significantly different based on Fisher's LSD at = 0.05

TABLE-US-00004 TABLE 4 Injury and stand reduction for four rice cultivars caused by ME acetochlor three weeks after crop emergence. ME Injury (%) Stand reduction (%) acetochlor.sup.a SDTR.sup.b Diamond Titan FP 7521 FP 7321 Diamond Titan FP 7521 FP 7321 0 0 0 C.sup.c 0 B 0 B 0 B 0 D 0 B 0 B 0 C 2.5 6 BC 5 AB 1 B 2 B 15 CD 5 B 12 AB 6 BC 5.0 5 BC 3 B 4 B 3 B 17 CD 7 B 18 AB 26 AB 1,260 0 17 A 30 A 24 A 24 A 39 A 40 A 34 A 35 A 2.5 12 AB 4 AB 5 B 5 AB 33 AB 18 AB 16 AB 23 AB 5.0 5 BC 5 AB 5 B 10 AB 17 B 18 AB 18 AB 16 ABC .sup.ag ai ha.sup.1 .sup.bFenclorim seed treatment (g ai kg.sup.1 seed) .sup.cMeans within a column followed by the same letter are not significantly different based on Fisher's LSD at = 0.05

Conclusions:

[0049] Results from the cultivar study demonstrate the safening effect of the fenclorim seed treatment. Safening effects can be observed from the groundcover data at six weeks after treatment (Table 3). As the fenclorim seed treatment rate was increased, stand loss and injury generally decreased (Table 4). The fenclorim seed treatment provided a positive response in canopy closure of rice cultivar Titan without the acetochlor treatment. Furthermore, in plots treated with acetochlor, the fenclorim seed treatment improved canopy closure, indicative of the safening by the seed treatment for all cultivars.

Example 5

Tolerance of Rice to ME Acetochlor Under Cold and Moist Soil Conditions

[0050] An experiment was conducted in Fall of 2019 to determine tolerance of rice to ME acetochlor when treated with a fenclorim rice seed treatment under cold and moist environmental conditions. The experiment was conducted within a growth chamber at 13.8 C. (night) and 23.8 C. (day) using a 10-hour night and 14-hour day photoperiod. Diamond rice was planted in pots filled to the same weight of soil to maintain 80% soil moisture throughout the experiment. Treatments consisted of an application of ME acetochlor at 0 and 1,050 g ai ha.sup.1 and a fenclorim seed treatment of 0 and 2.5 g ai kg.sup.1 seed. Data were analyzed using R and subjected to ANOVA. Means were separated using Fisher's LSD (=0.05).

TABLE-US-00005 TABLE 5 Injury and stand reduction to rice caused by ME acetochlor in a growth chamber experiment under adverse conditions for rice emergence and growth. 3 WAA.sup.a 4 WAA Stand ME reduction.sup.d Injury Height Biomass acetochlor.sup.b SDTR.sup.c (%) (%) (cm) (%) 0 0 0 B.sup.e 0 B 14.1 A 100 A 2.5 3 B 0 B 13.7 A 99 A 1,050 0 38 A 32 A 11.1 B 68 B 2.5 4 B 3 B 13.8 A 105 A .sup.aWeeks after application .sup.brate in g ai ha.sup.1 .sup.cFenclorim seed treatment (g ai kg.sup.1-seed) .sup.dStand reduction, injury, and biomass were made relative to the 0 acetochlor and 0 fenclorim treatments .sup.eMeans within a column followed by the same letter are not significantly different based on Fisher's LSD at = 0.05

[0051] Under cool, moist conditions, the fenclorim seed treatment provided adequate safety to ME acetochlor (Table 5). In evaluations taken at three weeks after application (WAA) and four WAA, rice treated with both the fenclorim seed treatment and ME acetochlor was not significantly different from nontreated rice. FIG. 5 illustrates the safening effect observed in the growth chamber.

Example 6

Weed Control and Rice Tolerance with ME Acetochlor and a Fenclorim Seed Treatment.

[0052] An experiment was conducted in the Spring of 2020 to determine the control of barnyardgrass and weedy rice in cultivated rice to ME acetochlor when treated with a fenclorim rice seed treatment. The experiment was conducted under standard small-plot research practices. Diamond rice was planted at 72 seeds m.sup.1 row. Treatments consisted of an application of ME acetochlor at four application timings (preemergence, delayed-preemergence, spiking, and 1-leaf), three application rates (630, 1,260, and 1,890 g ai ha.sup.1), and a fenclorim seed treatment of 0 and 2.5 g ai kg.sup.1-seed. Data were analyzed using R and subjected ANOVA. Means were separated using Fisher's LSD (=0.05).

TABLE-US-00006 TABLE 6 Weedy rice and barnyardgrass control 3 WAA +/ 3 days. Application Acetochlor rate BYG.sup.b control WR.sup.c control timing (g ai ha.sup.1) (%) (%) Preemergence 630 56 CD.sup.d 36 CD 1,260 83 A 56 AB 1,890 86 A 60 AB Delayed- 630 73 ABC 47 BC Preemergence 1,260 88 A 71 A 1,890 86 A 63 AB Spiking 630 76 ABC 20 D 1,260 61 BCD 29 CD 1,890 80 AB 59 AB 1 - Leaf 630 58 CD 31 CD 1,260 43 D 26 D 1,890 69 ABC 56 AB .sup.a Seed treatment g ai kg.sup.1-seed .sup.bBarnyardgrass .sup.cWRWeedy rice or red rice .sup.dMeans within a column followed by the same letter are not significantly different based on Fisher's LSD at = 0.05

TABLE-US-00007 TABLE 7 Cultivated rice injury caused by ME acetochlor averaged over application timing three WAA +/ 3 days Acetochlor rate Fenclorim (g ai ha.sup.1) SDTR.sup.a Injury 630 0 17 BC.sup.b 2.5 8 C 1,260 0 38 A 2.5 16 BC 1,890 0 48 A 2.5 20 B .sup.aSeed treatment g ai kg.sup.1-seed .sup.bMeans within a column followed by the same letter are not significantly different based on Fisher's LSD at = 0.05

[0053] For PRE and DPRE applications of ME acetochlor (1,260 and 1,890 g ai ha.sup.1), the herbicide provided greater than 80% barnyardgrass control and greater than 50% weedy rice control (Table 6). In the absence of a fenclorim seed treatment, it would be impossible to safely remove weedy rice from cultivated rice. Additionally, the fenclorim seed treatment also reduced the level of injury observed from ME acetochlor applications, and the seed treatment did not negatively affect weed control (Table 7).