A METHOD OF CONTROLLING HERBICIDE RESISTANT WEEDS

Abstract

The present invention relates to a method for effectively controlling herbicide resistant weeds such as Spermacoce verticillate. The present invention provides a method of reducing the population Spermacoce verticillate weeds by applying sequentially a herbicide combination to target said weeds.

Claims

1. A method of controlling herbicide resistant weeds by applying sequentially to a locus a combination of herbicides comprising, (iii) a first application comprising a combination of glyphosate and/or one or more herbicides, and (iv) a second application comprising a combination of glufosinate and/or one or more herbicides, wherein the second application of the combination of herbicides is applied atleast seven days after the first application.

2. The method as claimed in claim 1, wherein the herbicide resistant weed is Spermacoce verticillate.

3. The method as claimed in claim 1, wherein the weeds are resistant to herbicides selected from the group comprising 5-enolpyruvylshikimate-3-phosphate synthase inhibiting herbicides and growth regulating herbicides.

4. The method as claimed in claim 3, wherein the 5-enolpyruvylshikimate-3-phosphate synthase inhibiting herbicide is glyphosate.

5. The method as claimed in claim 3, wherein the growth regulating herbicides are selected from the group comprising dichlorprop (2,4-DP), methylchlorophenoxypropionic acid, 2-methyl-4-chlorophenoxyacetic acid and the like.

6. The method as claimed in claim 1, wherein the first application comprises a herbicide combination comprising glyphosate and triclopyr.

7. The method as claimed in claim 1, wherein glufosinate is applied as a salt selected from the group comprising potassium, sodium, ammonium, trimethylsulfonium, monoethanol ammonium, isopropylammonium, and triazolamine salt.

8. The method as claimed in claim 1, wherein the glufosinate herbicide is L-glufosinate.

9. The method as claimed in claim 8, wherein the glufosinate herbicide is L-glufosinate ammonium.

10. The method as claimed in claim 1, wherein the second application comprises applying a combination of glufosinate and one or more herbicides selected from flumioxazin, imazethapyr, metolachlor and carfentrazone to a locus.

11. The method as claimed in claim 1, wherein the second application is applied at least 14 days after the first application.

12. The method as claimed in claim 1, wherein the second application is applied at least 30 days after the first application.

13. The method as claimed in claim 1, wherein the concentration of one or more herbicides of the first application is ranging from 500 to 1500 g/ha.

14. The method as claimed in claim 1, wherein the concentration of glufosinate is ranging from 200 to 800 g/ha.

15. The method as claimed in claim 1, wherein the concentration of one or more herbicides of the second application is ranging from 10 to 1200 g/ha.

16. The method as claimed in claim 1, wherein the herbicide combinations are applied at a rate of 0.1 to 10 L/ha.

17. The method as claimed in claim 16, wherein the herbicide combinations are applied at a rate of 1 to 5 L/ha.

18. A method of controlling Spermacoce verticillate weeds resistant to herbicides comprising (i) a first application comprising a combination of glyphosate and/or one or more herbicides, and (ii) a second application comprising a combination of glufosinate and/or one or more herbicides, wherein the second application of the combination of herbicides is applied atleast 14 days after the first application.

19. The method of controlling Spermacoce verticillate weeds resistant to herbicides as claimed in claim 18, said method comprising (i) a first application comprising a combination of glyphosate and triclopyr. (ii) a second application comprising a combination of glufosinate and/or one or more herbicides selected from flumioxazin, imazethapyr, metolachlor and carfentrazone. wherein the second application of the combination of herbicides is applied atleast 14 days after the first application.

20. The method of controlling Spermacoce verticillate weeds resistant to herbicides as claimed in claim 18, said method comprising (i) a first application comprising a combination of glyphosate and triclopyr, and (ii) a second application comprising glufosinate wherein the second application of the combination of herbicides is applied atleast 14 days after the first application.

21. The method of controlling Spermacoce verticillate weeds resistant to herbicides as claimed in claim 18, said method comprising (i) a first application comprising a combination of glyphosate and triclopyr, and (ii) a second application comprising glufosinate and flumioxazin, wherein the second application of the combination of herbicides is applied atleast 14 days after the first application.

22. The method of controlling Spermacoce verticillate weeds resistant to herbicides as claimed in claim 18, said method comprising, (i) a first application comprising a combination of glyphosate and triclopyr, and (ii) a second application comprising glufosinate and metolachlor, wherein the second application of the combination of herbicides is applied atleast 14 days after the first application.

23. The method of controlling Spermacoce verticillate weeds resistant to herbicides as claimed in claim 18, said method comprising, (iii) a first application comprising a combination of glyphosate and triclopyr, and (iv) a second application comprising glufosinate, imazethapyr and carfentrazone, wherein the second application of the combination of herbicides is applied atleast 14 days after the first application.

24. A kit-of-parts comprising a first component of a combination of glyphosate and triclopyr, and a second component of a combination of glufosinate or its salts and/or one or more herbicides selected from flumioxazin, imazethapyr, metolachlor and carfentrazone.

25. The kit-of-parts as claimed in claim 24, wherein said kit-of-parts effectively controls herbicide resistant Spermacoce verticillate weeds.

Description

DESCRIPTION OF PRESENT INVENTION

[0029] For the purposes of the following detailed description, it is to be understood that the invention may assume various alternative variations, except where expressly specified to the contrary. Moreover, other than in any operating examples, or where otherwise indicated, all numbers expressing, for example, quantities of materials/ingredients used in the specification are to be understood as being modified in all instances by the term about. The term about used to qualify the amounts of active components shall be interpreted to mean approximately or reasonably close to and any statistically insignificant variations therefrom.

[0030] As used herein, the terms comprising including, having, containing, involving, and the like are to be understood to be open-ended, i.e., to mean including but not limited to.

[0031] In any aspect or embodiment described hereinbelow, the phrase comprising may be replaced by the phrases consisting of or consisting essentially of or consisting substantially of. In these aspects or embodiment, the combination or composition described includes or comprises or consists of or consists essentially of or consists substantially of the specific components recited therein, to the exclusion of other ingredients or excipients not specifically recited therein.

[0032] The term herbicide, as used herein, shall mean an active ingredient that kills, controls or otherwise adversely modifies the growth of undesired plants.

[0033] As used herein, a herbicidally effective or vegetation controlling amount is an amount of active ingredient that causes a herbicidal effect, i.e., an adversely modifying effect and includes deviations from natural development, killing, regulation, desiccation, retardation.

[0034] The terms plants and vegetation include, but are not limited to, germinant seeds, emerging seedlings, plants emerging from vegetative propagules, and established vegetation.

[0035] The terms undesirable vegetation, harmful plants, unwanted plants, weeds and weed species, as used herein, are synonyms.

[0036] The term locus as used herein shall denote the vicinity of a desired crop in which weed control, typically selective weed control is desired. The locus includes the vicinity of desired crop plants wherein the weed infestation has either emerged or is yet to emerge.

[0037] The term crop shall include a multitude of desired crop plants or an individual crop plant growing at a locus.

[0038] A used herein, a reference to Group 4 herbicides includes references to synthetic auxins which are chemicals disrupting plant cell growth in newly forming stems and leaves (malformed growth). These herbicides include phenoxy-carboxylic acids (e.g., 2,4-dichlorophenoxyacetic acid); dichlorprop (2,4-DP), methylchlorophenoxypropionic acid, 2-methyl-4-chlorophenoxyacetic acid and the like. benzoic acids (e.g., dicamba); pyridine acids (e.g., picloram, clopyralid); quinoline carboxylic acids (e.g., quinclorac); and pyrimidine carboxylic acid (e.g. aminocyclopyrachlor).

[0039] A used herein, a reference to Group 9 herbicides includes references to inhibitors of 5-enolpyruvylshikimate-3-phosphate synthase (EPSP). These chemicals inhibit amino acid synthesis through the shikimate pathway

[0040] Each of the aspect described above may have one or more embodiments.

[0041] Each of the embodiments described hereinafter may apply to one or all the aspects described hereinabove. These embodiments are intended to be read as being preferred features of one or all the aspects described hereinabove. Each of the embodiments described hereinafter applies to each of the aspects described hereinabove individually.

[0042] Weed resistance or tolerance to herbicides has been increasing in Brazil and around the world, and new ways of using the available products is essential to manage this problem. Spermacoce verticillate (S. verticillate) has been reported as one of the important weeds in annual and perennial crops in the North and Midwest of Brazil, largely due to their tolerance to 2,4-D synthetic auxin herbicides and glyphosate that are the main herbicides used at broadleaves burndown. Therefore, the control becomes insufficient with a single application of these herbicides alone or in mixtures such as with glyphosate and 2,4-D.

[0043] Some herbicides are efficient for controlling Spermacoce verticillate, but this efficiency is restricted only to the early stages of development (4 to 6 leaves), adult plants with more than 6 leaves showing side shoots and in the flowering stage are not satisfactorily controlled even after single or sequential applications of herbicides. Farmers have been facing this problem and have no alternative methods to control this weed (S. verticillate) when it reaches the later stages of development, as S. verticillata is relatively resistant to many of the most common herbicides including 2,4-D, MCPA, atrazine and metolachlor. The present invention is aimed to solve this problem.

[0044] Surprisingly, it has been found by the present inventors that a sequential application of a combination of glufosinate and/or one or more herbicides after an application of glyphosate and/or one or more herbicides is used to effectively control S. verticillate, a weed which grows in main agricultural areas and that is tolerant/resistant to main herbicides used for burndown, for example glyphosate, 2,4-D and other auxin herbicides.

[0045] The present method results in an effective control of herbicide resistant weeds at the locus of the desirable crop plants when the combination of herbicides is applied in a sequential manner. The resulting phytotoxicity usually seen with application of glyphosate and glufosinate or combinations thereof for controlling weeds is also absent when these herbicides are applied in a sequential manner.

[0046] According to the present invention, there is provided an effective and quick method to control S. verticillate weeds. The method comprises applying herbicides in a sequential application which efficiently controls this weed.

[0047] In an embodiment, the present invention provides preferred combinations, compositions and methods thereof. The methods of the invention include a method of controlling weeds at a locus by applying to the locus the combination or the composition, or a method of increasing yield in a crop by application of the combination or composition, or a method of improving the plant health by application at the locus of the plant the combination or the composition. The embodiments described herein describe the preferred embodiments of all these possible combinations, compositions and methods of the invention.

[0048] In an aspect the present invention relates to herbicidal combinations. The invention also relates to methods and uses for controlling undesirable vegetation, particularly in crop fields.

[0049] In another aspect the present invention provides an effective control of herbicide-resistant weeds, wherein the herbicide resistant weeds are resistant to at least one herbicide belonging to Group 9 and Group 4.

[0050] Typically, the target weeds of present invention are tolerant to glyphosate, auxin herbicides and the like.

[0051] The present invention provides a method for controlling herbicide resistant weeds, particularly, Spermacoce verticillate.

[0052] In a preferred embodiment the herbicide tolerant weed is Spermacoce verticillate.

[0053] In another aspect, the invention provides a highly effective and quick method for controlling Spermacoce verticillate that is tolerant to herbicides belonging to Group 9 and Group 4.

[0054] In another aspect, the invention provides a highly effective and quick method for controlling Spermacoce verticillate that is tolerant to glyphosate (Group 9) and auxin herbicides (Group 4) such as phenoxy-carboxylic acids (e.g., 2,4-dichlorophenoxyacetic acid); benzoic acids (e.g., dicamba); pyridine acids (e.g., picloram, clopyralid); quinoline carboxylic acids (e.g., quinclorac); and pyrimidine carboxylic acid (e.g. aminocyclopyrachlor).

[0055] In an embodiment, the present invention provides a method of controlling herbicide resistant weeds by applying sequentially to a locus a combination of herbicides comprising [0056] (i) a first application comprising a combination of glyphosate and/or one or more herbicides, and [0057] (ii) a second application comprising a combination of glufosinate and/or one or more herbicides.

[0058] The present invention provides that an application of glyphosate and/or one or more herbicides at a locus of a crop, followed by an application of glufosinate and/or one or more herbicides at a locus of the said crop, provides for an excellent control of Spermacoce verticillate weeds.

[0059] In an embodiment, the preferred glufosinate herbicide is L-glufosinate.

[0060] Accordingly, the present inventors have surprisingly found that an application of a combination of herbicides particularly, an application of glyphosate and triclopyr, followed by an application of glufosinate or mixtures of glufosinate provides for an excellent control of S. verticillate weed.

[0061] In an aspect, the weeds are resistant to herbicides selected from the group comprising 5-enolpyruvylshikimate-3-phosphate synthase inhibiting herbicides and growth regulating herbicides.

[0062] In an embodiment, the weeds are resistant to a 5-enolpyruvylshikimate-3-phosphate synthase inhibiting herbicide, glyphosate.

[0063] In another embodiment, the Spermacoce verticillate weeds are resistant to growth regulating herbicides selected from the group comprising auxin herbicides (Group 4) such as phenoxy-carboxylic acids (e.g., 2,4-dichlorophenoxyacetic acid); benzoic acids (e.g., dicamba); pyridine acids (e.g., picloram, clopyralid); quinoline carboxylic acids (e.g., quinclorac); and pyrimidine carboxylic acid (e.g. aminocyclopyrachlor).

[0064] In an embodiment, the first application comprises a combination of glyphosate and one or more herbicides selected from glyphosate and one or more herbicides of growth regulating herbicides.

[0065] In an embodiment, the first application comprises a combination of glyphosate and triclopyr.

[0066] In an embodiment the herbicide applied sequentially is glufosinate or its salts.

[0067] In an embodiment, the herbicide applied sequentially is L-glufosinate or its salts.

[0068] Preferably, the herbicide sequentially applied comprises L-glufosinate in its free form or in salt form, in particular potassium, sodium, ammonium, trimethylsulfonium, monoethanol ammonium, isopropylammonium, triazolamine salt or any other agrochemically acceptable salt. Preferably, glufosinate ammonium is used as selective herbicide.

[0069] In an embodiment, the preferred glufosinate herbicide applied sequentially is L-glufosinate or L-glufosinate ammonium and/or one or more herbicides.

[0070] In another aspect, the present invention provides a method of controlling weeds resistant to herbicides wherein said weeds are treated by applying concurrently or sequentially, herbicidally effective amounts of at least two herbicides to target weeds.

[0071] In another aspect, the present invention provides a method of controlling weeds resistant to herbicides, wherein said weeds are controlled by applying to a locus of crops, concurrently or sequentially, a composition comprising a herbicidally effective amount of a combination of herbicides.

[0072] In an embodiment, the present invention provides a method of controlling weeds resistant to herbicides belonging to Group 4 and Group 9, the method comprising applying glyphosate and/or one or more herbicides at a locus of a crop with a sequential application of glufosinate and/or one or more herbicides to effectively control herbicide resistant Spermacoce verticillate weeds.

[0073] Preferably, the method comprises applying glyphosate and triclopyr with a sequential application of L-glufosinate or glufosinate and one or more herbicides to effectively control herbicide resistant weed, which is Spermacoce verticillate.

[0074] In an embodiment, the second application comprises a combination of L-glufosinate or its salts and one or more herbicides selected from the group comprising flumioxazin, imazethapyr, metolachlor and carfentrazone.

[0075] In one preferred embodiment, the second application comprises a combination of L-glufosinate ammonium and one or more herbicides selected from the group comprising flumioxazin, imazethapyr, metolachlor and carfentrazone.

[0076] In an embodiment the method of present invention significantly controls the herbicide resistant weeds within a period of 10 to 30 days.

[0077] In an embodiment the method comprises applying a herbicidally effective amount of the combination of herbicides within a period of 10 to 30 days.

[0078] In an embodiment the method comprises applying a herbicidally effective amount of the combination of herbicides concurrently or sequentially within a period of 10 to 30 days, preferably within a period of 10 to 15 days.

[0079] According to the method of the present invention adult plants of Spermacoce verticillate that are resistant to herbicides, particularly herbicides of Group 4 and Group 9 is controlled with the application of glyphosate and triclopyr followed by an application of glufosinate after 10 to 30 days of the first application, preferably within a period of 10 to 15 days.

[0080] According to the method of the present invention adult plants of Spermacoce verticillate that are resistant to herbicides, particularly herbicides of Group 4 and Group 9 is controlled with the application of glyphosate and triclopyr followed by an application of L-glufosinate or its salts after 10 to 30 days of the first application, preferably within a period of 10 to 15 days.

[0081] According to the method of the present invention, adult plants of Spermacoce verticillate that are resistant to herbicides, particularly herbicides of Group 4 and Group 9 are controlled with an application of glyphosate and triclopyr followed by an application of a combination of herbicides selected from one or more of glufosinate and flumioxazin after 10 to 30 days, preferably within a period of 10 to 15 days.

[0082] According to the method of the present invention, adult plants of Spermacoce verticillate that are resistant to herbicides, particularly herbicides of Group 4 and Group 9 are controlled with an application of glyphosate and triclopyr followed by an application of a combination of herbicides selected from one or more of L-glufosinate or its salts and flumioxazin after 10 to 30 days, preferably within a period of 10 to 15 days.

[0083] According to the method of the present invention, adult plants of Spermacoce verticillate that are resistant to herbicides, particularly herbicides of Group 4 and Group 9 are controlled with an application of glyphosate and triclopyr followed by an application of a combination of herbicides selected from one or more of glufosinate and metolachlor after 10 to 30 days, preferably within a period of 10 to 15 days.

[0084] According to the method of the present invention, adult plants of Spermacoce verticillate that are resistant to herbicides, particularly herbicides of Group 4 and Group 9 are controlled with an application of glyphosate and triclopyr followed by an application of a combination of herbicides selected from one or more of L-glufosinate or its salts and metolachlor after 10 to 30 days, preferably within a period of 10 to 15 days.

[0085] According to the method of the present invention, adult plants of Spermacoce verticillate that is resistant to herbicide is controlled with the application of glyphosate and triclopyr followed by application of a combination of herbicides selected from glufosinate, imazethapyr and carfentrazone after 10 to 30 days, preferably within a period of 10 to 15 days.

[0086] According to the method of the present invention, adult plants of Spermacoce verticillate that is resistant to herbicide is controlled with the application of glyphosate and triclopyr followed by application of a combination of herbicides selected from L-glufosinate or its salts, imazethapyr and carfentrazone after 10 to 30 days, preferably within a period of 10 to 15 days.

[0087] According to the method of the present invention adult plants of Spermacoce verticillate that is resistant to herbicide is controlled with the application of glyphosate and triclopyr followed by application of herbicides selected from glufosinate and carfentrazone after 10 to 30 days, preferably within a period of 10 to 15 days.

[0088] According to the method of the present invention adult plants of Spermacoce verticillate that is resistant to herbicide is controlled with the application of glyphosate and triclopyr followed by application of herbicides selected from L-glufosinate or its salts and carfentrazone after 10 to 30 days, preferably within a period of 10 to 15 days.

[0089] According to the method of the present invention adult plants of Spermacoce verticillate that is resistant to herbicide is controlled with the application of glyphosate and triclopyr followed by an application of herbicides selected from glufosinate and imazethapyr after 10 to 30 days, preferably within a period of 10 to 15 days.

[0090] According to the method of the present invention adult plants of Spermacoce verticillate that is resistant to herbicide is controlled with the application of glyphosate and triclopyr followed by an application of herbicides selected from L-glufosinate or its salts and imazethapyr after 10 to 30 days, preferably within a period of 10 to 15 days.

[0091] The combination may be applied to the locus of the weeds in herbicidally effective amounts. The selection of the appropriate effective amounts depends on the density of weed infestation, weather patterns, crop health and many other factors.

[0092] In an embodiment, glufosinate or glyphosate or their salts may be used in an amount of from about 1 g/ha to about 5000 g/ha.

[0093] In an embodiment, glufosinate or its salts may be used in an amount from about 5 g/ha to about 800 g/ha.

[0094] In an embodiment, glufosinate or its salts may be used in an amount about 100 g/ha to 400 g/ha.

[0095] In an embodiment, L-glufosinate or its salts may be used in an amount from about 5 g/ha to about 800 g/ha.

[0096] In an embodiment, the concentration of one or more herbicides of the second application is ranging from 10 to 1200 g/ha.

[0097] In an embodiment, the concentration of one or more herbicides of the first application is ranging from 500 to 1500 g/ha.

[0098] In an embodiment, the herbicides of the present invention may be applied with at least one other active ingredient selected from the group consisting of herbicides, insecticides, fungicides, biological agents, plant growth activators, fertilizers or combinations thereof.

[0099] The compositions may further comprise at least one agrochemically acceptable excipient.

[0100] The dosage level of the individual herbicides may be defined by the inherent efficacy of the compound, the pest pressure, soil type, the weather, the formulation type and many other indeterminate variables. These standard dosage levels are fixed by the regulatory authorities.

[0101] These compositions can be applied by spraying onto the weeds to be treated. The application dose will of course depend on the nature of the crop and the weed to be treated. For example, these herbicide combinations, compositions can be applied at a rate of 0.1 to 10 L/ha, preferably from 0.5 to 8 L/ha, and even more preferably from 1 to 5 L/ha, typically 1 L/ha to 3 L/ha of crop to be treated.

[0102] In an embodiment, the individual components of the combination of the present invention may be applied to the locus either simultaneously or sequentially, such that glyphosate and mixture as well as glufosinate and mixture as mentioned herein above may be applied in a tank mix or as a pre-mixed composition within a period of 10 to 30 days of each other.

[0103] The herbicidal compositions of the present disclosure can be in any conventional agriculturally useful form, for example, in the form of a ready-to-use formulation, or in the form of a tank mix.

[0104] In an embodiment, the herbicidal compositions of the present disclosure are tank mixes.

[0105] In an embodiment, combination of the present invention may be applied either pre or post emergent. The advantage of the combination is surprisingly good residual effects, when applied in post-emergent leading to quick control of weeds.

[0106] In an embodiment, the composition of the present invention may contain agriculturally acceptable adjuvants, carriers, diluents, emulsifiers, fillers, antifoaming agents, thickening agents, anti-freezing agents, freezing agents etc. The compositions may be either solid or liquids. They can be solids, such as, for example, dusts, granules, water-dispersible granules, microcapsules or wettable powders, or liquids, such as, for example, emulsifiable concentrates, solutions, emulsions or suspensions, ZC formulations. They can also be provided as a pre-mix or tank mixes.

[0107] Suitable agricultural adjuvants and carriers may include, but are not limited to, crop oil concentrates; methylated seed oils, emulsified methylated seed oil, nonylphenol ethoxylate; benzylcocoalkyldimethyl quaternary ammonium salt; blend of petroleum hydrocarbon, alkyl esters, organic acid, and anionic surfactant; C.sub.9-C.sub.11 alkylpolyglycoside; phosphated alcohol ethoxylate; natural primary alcohol (C.sub.12-C.sub.16) ethoxylate; di-sec-butylphenol EO-PO block copolymer; polysiloxane-methyl cap; nonylphenol ethoxylate, urea ammonium nitrate; tridecyl alcohol (synthetic) ethoxylate (8EO); tallow amine ethoxylate; PEG(400) dioleate-99, alkyl sulfates, such as diethanolammonium lauryl sulfate; alkylarylsulfonate salts, such as calcium dodecylbenzenesulfonate; alkylphenol-alkylene oxide addition products, such as nonylphenol-C.sub.18 ethoxylate; alcohol-alkylene oxide addition products, such as tridecyl alcohol-C.sub.16 ethoxylate; soaps, such as sodium stearate; alkyl-naphthalene-sulfonate salts, such as sodium dibutylnaphthalenesulfonate; dialkyl esters of sulfosuccinate salts, such as sodium di(2-ethylhexyl)sulfosuccinate; sorbitol esters, such as sorbitol oleate; quaternary amines, such as lauryl trimethylammonium chloride; polyethylene glycol esters of fatty acids, such as polyethylene glycol stearate; block copolymers of ethylene oxide and propylene oxide; salts of mono and dialkyl phosphate esters; vegetable or seed oils such as soybean oil, rapeseed/canola oil, olive oil, castor oil, sunflower seed oil, coconut oil, corn oil, cottonseed oil, linseed oil, palm oil, peanut oil, safflower oil, sesame oil, tung oil and the like; and esters of the above vegetable oils, and in certain embodiments, methyl esters.

[0108] Suitable liquid carriers that may be employed in a composition of the present invention may include water or organic solvents. The organic solvents include, but are not limited to, petroleum fractions or hydrocarbons such as mineral oil, aromatic solvents, paraffinic oils, and the like; vegetable oils such as soybean oil, rapeseed oil, olive oil, castor oil, sunflower seed oil, coconut oil, corn oil, cottonseed oil, linseed oil, palm oil, peanut oil, safflower oil, sesame oil, tung oil and the like; esters of the above vegetable oils; esters of monoalcohols or dihydric, trihydric, or other lower polyalcohols (4-6 hydroxy containing), such as 2-ethyl hexyl stearate, n-butyl oleate, isopropyl myristate, propylene glycol dioleate, di-octyl succinate, di-butyl adipate, di-octyl phthalate and the like; esters of mono, di and polycarboxylic acids and the like. Organic solvents include, but are not limited to toluene, xylene, petroleum naphtha, crop oil, acetone, methyl ethyl ketone, cyclohexanone, trichloroethylene, perchloroethylene, ethyl acetate, amyl acetate, butyl acetate, propylene glycol monomethyl ether and diethylene glycol monomethyl ether, methyl alcohol, ethyl alcohol, isopropyl alcohol, amyl alcohol, ethylene glycol, propylene glycol, glycerine, N-methyl-2-pyrrolidinone, N,N-dimethyl alkylamides, dimethyl sulfoxide.

[0109] Solid carriers that may be employed in the compositions of the present invention may include but are not limited to attapulgite, pyrophyllite clay, silica, kaolin clay, kieselguhr, chalk, diatomaceous earth, lime, calcium carbonate, bentonite clay, Fuller's earth, talc, cottonseed hulls, wheat flour, soybean flour, pumice, wood flour, walnut shell flour, lignin, cellulose etc.

[0110] Other exemplary additives which can be used in the compositions provided herein include, but not limited to, at least one compatibilizers, antifoaming agents, sequestering agents, neutralizing agents and buffers, corrosion inhibitors, coloring agents, odorants, spreading agents, permeation aids, fixing agents, dispersants, thickeners, freezing point depressants, anti-microbial agents etc.

[0111] In another aspect, method may further comprise applying at least one adjuvant or at least one safener.

[0112] In accordance with present invention the method of control of target weeds may be carried out by spraying the suggested tank mixes, or the individual herbicides may be formulated as a kit-of-parts containing various components that may be mixed as instructed prior to spraying.

[0113] The herbicidal combinations of the present invention maybe used to target weeds selected from the group comprising Echinochloa crus-galli, Setaria viridis, Setaria faberi, Setaria glauca, Setaria geniculata, Digitaria ciliaris, Digitaria sanguinalis, Digitaria horizontalis, Digitaria insularis, Eleusine indica, Poa annua, Alospecurus aequalis, Alopecurus myosuroides, Avena fatua, Sorghum halepense, Sorghum vulgare, Agropyron repens, Lolium multiflorum, Lolium perenne, Lolium rigidum, Bromus secalinus, Bromus tectorum, Hordeum jubatum, Aegilops cylindrica, Phalaris arundinacea, Phalaris minor, Apera spica-venti, Panicum dichotomiflorum, Panicum texanum, Panicum maximum, Brachiaria platyphylla, Brachiaria ruziziensis, Brachiaria plantaginea, Brachiaria decumbens, Brachiaria brizantha, Brachiaria humidicola, Cenchrus echinatus, Cenchrus pauciflorus, Eriochloa villosa, Pennisetum setosum, Chloris gayana, Eragrostis pilosa, Rhynchelitrum repens, Dactyloctenium aegyptium, Ischaemum rugosum, Oryza sativa, Paspalum notatum, Paspalum maritimum, Pennisetum clandestinum, Pennisetum setosum or Rottboellia cochinchinensis.

[0114] The herbicidal combinations of the present invention maybe used to target weeds among the crops such as corn, rice, wheat, barley, rye, oat, sorghum, cotton, soybean, peanut, buckwheat, beet, rapeseed, sunflower, sugar cane, tobacco, etc.; vegetables: solanaceous vegetables such as eggplant, tomato, pimento, pepper, potato, etc., cucurbit vegetables such as cucumber, pumpkin, zucchini, water melon, melon, squash, etc., cruciferous vegetables such as radish, white turnip, horseradish, kohlrabi, Chinese cabbage, cabbage, leaf mustard, broccoli, cauliflower, etc., asteraceous vegetables such as burdock, crown daisy, artichoke, lettuce, etc, liliaceous vegetables such as green onion, onion, garlic, and asparagus, ammiaceous vegetables such as carrot, parsley, celery, parsnip, etc., chenopodiaceous vegetables such as spinach, Swiss chard, etc., lamiaceous vegetables such as Perilla frutescens, mint, basil, etc, strawberry, sweet potato, Dioscorea japonica, colocasia, etc., flowers, foliage plants, turf grasses, fruits: pome fruits such apple, pear, quince, etc, stone fleshy fruits such as peach, plum, nectarine, Prunus mume, cherry fruit, apricot, prune, etc., citrus fruits such as orange, lemon, rime, grapefruit, etc., nuts such as chestnuts, walnuts, hazelnuts, almond, pistachio, cashew nuts, macadamia nuts, etc. berries such as blueberry, cranberry, blackberry, raspberry, etc., vines, kaki fruit, olive, plum, banana, oil palm, coffee, date palm, coconuts, etc., trees other than fruit trees; tea, mulberry, flowering plant, trees such as ash, birch, dogwood, Eucalyptus, Ginkgo biloba, lilac, maple, Quercus, poplar, Judas tree, Liquidambar formosana, plane tree, zelkova, Japanese arborvitae, fir wood, hemlock, juniper, Pinus, Picea, and Taxus cuspidate, etc.

[0115] The target weeds are selected from family of Spermacoce weeds.

[0116] Preferably the target weed is Spermacoce verticillate.

[0117] The method of controlling Spermacoce verticillate weeds according to the present invention wherein said method comprises applying concurrently or subsequently or sequentially herbicide combinations to target weeds.

[0118] In an embodiment, the adult plants of Spermacoce verticillate which is resistant to herbicides like glyphosate and auxin herbicide like 2,4 D, can be effectively controlled by sequential application of glyphosate and at least 7 days, typically about 10 to 15 days later application of L-glufosinate or its salts and/or one or more herbicides with at least one other herbicide to target herbicide resistant weeds.

[0119] In an embodiment, the adult plants of Spermacoce verticillate which is resistant to herbicides like glyphosate and growth regulating herbicide like 2,4 D, can be effectively controlled by sequential application of glyphosate and at least 7 days, typically about 10 to 15 days later application of L-glufosinate or its salts and/or one or more herbicides with at least one other herbicide to target herbicide resistant weeds.

[0120] Therefore, in an embodiment, the present invention provides a method of controlling weeds at a locus, the method comprising: [0121] (a) applying glyphosate and triclopyr at the target locus; and [0122] (b) within about 10 to about 15 days, applying glufosinate to the locus.

[0123] In another embodiment, the present invention provides a method of controlling weeds at a locus, the method comprising: [0124] (a) applying glyphosate and triclopyr at the target locus; and [0125] (b) within about 10 to about 15 days, applying combination of glufosinate with another herbicide to the locus.

[0126] In another embodiment, the present invention provides a method of controlling weeds at a locus, the method comprising: [0127] (a) applying glyphosate and triclopyr to the locus; and [0128] (b) applying glufosinate to the locus, wherein glufosinate is applied at least 7 days after the application of glyphosate and triclopyr.

[0129] In another embodiment, the present invention provides a method of controlling weeds at a locus, the method comprising: [0130] (a) applying glyphosate and triclopyr to the locus; and [0131] (b) applying glufosinate to the locus, wherein glufosinate is applied at least 14 days after the application of glyphosate and triclopyr.

[0132] In another embodiment, the present invention provides a method of controlling weeds at a locus, the method comprising: [0133] (a) applying glyphosate and triclopyr to the locus; and [0134] (b) applying glufosinate to the locus, wherein glufosinate is applied at least 21 days after the application of glyphosate and triclopyr.

[0135] In another embodiment, the present invention provides a method of controlling weeds at a locus, the method comprising: [0136] (a) applying glyphosate and triclopyr to the locus; and [0137] (b) applying glufosinate to the locus, wherein glufosinate is applied at least 28 days after the application of glyphosate and triclopyr.

[0138] In another embodiment, the present invention provides a method of controlling weeds at a locus, the method comprising: [0139] (a) applying glyphosate and triclopyr to the locus; and [0140] (b) applying L-glufosinate to the locus, wherein L-glufosinate is applied at least 7 days after the application of glyphosate and triclopyr.

[0141] In another embodiment, the present invention provides a method of controlling weeds at a locus, the method comprising: [0142] (a) applying glyphosate and triclopyr to the locus; and [0143] (b) applying glufosinate and flumioxazin to the locus, wherein glufosinate and flumioxazin is applied at least 7 days after the application of glyphosate and triclopyr.

[0144] In another embodiment, the present invention provides a method of controlling weeds at a locus, the method comprising: [0145] (a) applying glyphosate and triclopyr to the locus; and [0146] (b) applying glufosinate and flumioxazin to the locus, wherein glufosinate and flumioxazin is applied at least 14 days after the application of glyphosate and triclopyr.

[0147] In another embodiment, the present invention provides a method of controlling weeds at a locus, the method comprising: [0148] (a) applying glyphosate and triclopyr to the locus; and [0149] (b) applying glufosinate and flumioxazin to the locus, wherein glufosinate and flumioxazin is applied at least 21 days after the application of glyphosate and triclopyr.

[0150] In another embodiment, the present invention provides a method of controlling weeds at a locus, the method comprising: [0151] (a) applying glyphosate and triclopyr to the locus; and [0152] (b) applying glufosinate and flumioxazin to the locus, wherein glufosinate and flumioxazin is applied at least 28 days after the application of glyphosate and triclopyr.

[0153] In another embodiment, the present invention provides a method of controlling weeds at a locus, the method comprising: [0154] (a) applying glyphosate and triclopyr to the locus; and [0155] (b) applying L-glufosinate and flumioxazin to the locus, wherein L-glufosinate and flumioxazin is applied at least 7 days after the application of glyphosate and triclopyr.

[0156] In another embodiment, the present invention provides a method of controlling weeds at a locus, the method comprising: [0157] (a) applying glyphosate and triclopyr to the locus; and [0158] (b) applying glufosinate and metolachlor to the locus, wherein glufosinate and metolachlor is applied at least 7 days after the application of glyphosate and triclopyr.

[0159] In another embodiment, the present invention provides a method of controlling weeds at a locus, the method comprising: [0160] (a) applying glyphosate and triclopyr to the locus; and [0161] (b) applying glufosinate and metolachlor to the locus, wherein glufosinate and metolachlor is applied at least 14 days after the application of glyphosate and triclopyr.

[0162] In another embodiment, the present invention provides a method of controlling weeds at a locus, the method comprising: [0163] (a) applying glyphosate and triclopyr to the locus; and [0164] (b) applying glufosinate and metolachlor to the locus, wherein glufosinate and metolachlor is applied at least 21 days after the application of glyphosate and triclopyr.

[0165] In another embodiment, the present invention provides a method of controlling weeds at a locus, the method comprising: [0166] (a) applying glyphosate and triclopyr to the locus; and [0167] (b) applying glufosinate and metolachlor to the locus, wherein glufosinate and metolachlor is applied at least 28 days after the application of glyphosate and triclopyr.

[0168] In another embodiment, the present invention provides a method of controlling weeds at a locus, the method comprising: [0169] (a) applying glyphosate and triclopyr to the locus; and [0170] (b) applying L-glufosinate and metolachlor to the locus, wherein L-glufosinate and metolachlor is applied at least 7 days after the application of glyphosate and triclopyr.

[0171] In another embodiment, the present invention provides a method of controlling weeds at a locus, the method comprising: [0172] (a) applying glyphosate and triclopyr to the locus; and [0173] (b) applying combination of glufosinate, imazethapyr and carfentrazone to the locus, wherein glufosinate, imazethapyr and carfentrazone is applied at least 7 days after the application of glyphosate and triclopyr.

[0174] In another embodiment, the present invention provides a method of controlling weeds at a locus, the method comprising: [0175] (a) applying glyphosate and triclopyr to the locus; and [0176] (b) applying combination of glufosinate, imazethapyr and carfentrazone to the locus, wherein glufosinate, imazethapyr and carfentrazone is applied at least 14 days after the application of glyphosate and triclopyr.

[0177] In another embodiment, the present invention provides a method of controlling weeds at a locus, the method comprising: [0178] (a) applying glyphosate and triclopyr to the locus; and [0179] (b) applying a combination of glufosinate, imazethapyr and carfentrazone to the locus, wherein glufosinate, imazethapyr and carfentrazone is applied at least 21 days after the application of glyphosate and triclopyr.

[0180] In another embodiment, the present invention provides a method of controlling weeds at a locus, the method comprising: [0181] (a) applying glyphosate and triclopyr to the locus; and [0182] (b) applying a combination of glufosinate, imazethapyr and carfentrazone to the locus, wherein glufosinate, imazethapyr and carfentrazone is applied at least 28 days after the application of glyphosate and triclopyr.

[0183] In another embodiment, the present invention provides a method of controlling weeds at a locus, the method comprising: [0184] (a) applying glyphosate and triclopyr to the locus; and [0185] (b) applying combination of L-glufosinate, imazethapyr and carfentrazone to the locus, wherein L-glufosinate, imazethapyr and carfentrazone is applied at least 7 days after the application of glyphosate and triclopyr.

[0186] In another embodiment, the present invention provides a method of controlling weeds at a locus, the method comprising: [0187] (a) applying a combination comprising glyphosate and triclopyr to the locus; and [0188] (b) applying a combination comprising glufosinate and carfentrazone to the locus, wherein glufosinate and carfentrazone is applied at least 7 days after the first application.

[0189] In another embodiment, the present invention provides a method of controlling weeds at a locus, the method comprising: [0190] (a) applying a combination comprising glyphosate and triclopyr to the locus; and [0191] (b) applying a combination comprising glufosinate and carfentrazone to the locus, wherein glufosinate and carfentrazone is applied at least 14 days after the first application.

[0192] In another embodiment, the present invention provides a method of controlling weeds at a locus, the method comprising: [0193] (a) applying a combination comprising glyphosate and triclopyr to the locus; and [0194] (b) applying a combination comprising glufosinate and carfentrazone to the locus, wherein glufosinate and carfentrazone is applied at least 21 days after the first application.

[0195] In another embodiment, the present invention provides a method of controlling weeds at a locus, the method comprising: [0196] (a) applying a combination comprising glyphosate and triclopyr to the locus; and [0197] (b) applying a combination comprising glufosinate and carfentrazone to the locus, wherein glufosinate and carfentrazone is applied at least 28 days after the first application.

[0198] In another embodiment, the present invention provides a method of controlling weeds at a locus, the method comprising: [0199] (a) applying a combination comprising glyphosate and triclopyr to the locus; and [0200] (b) applying a combination comprising L-glufosinate and carfentrazone to the locus, wherein L-glufosinate and carfentrazone is applied at least 7 days after the first application.

[0201] In another embodiment, the present invention provides a method of controlling weeds at a locus, the method comprising: [0202] (a) applying a combination comprising glyphosate and triclopyr to the locus; and [0203] (b) applying a combination comprising glufosinate and imazethapyr to the locus, wherein glufosinate and imazethapyr is applied at least 7 days after the first application.

[0204] In another embodiment, the present invention provides a method of controlling weeds at a locus, the method comprising: [0205] (a) applying a combination comprising glyphosate and triclopyr to the locus; and [0206] (b) applying a combination comprising glufosinate and imazethapyr to the locus, wherein glufosinate and imazethapyr is applied at least 14 days after the first application.

[0207] In another embodiment, the present invention provides a method of controlling weeds at a locus, the method comprising: [0208] (a) applying a combination comprising glyphosate and triclopyr to the locus; and [0209] (b) applying a combination comprising glufosinate and imazethapyr to the locus, wherein glufosinate and imazethapyr is applied at least 21 days after the first application.

[0210] In another embodiment, the present invention provides a method of controlling weeds at a locus, the method comprising: [0211] (a) applying a combination comprising glyphosate and triclopyr to the locus; and [0212] (b) applying a combination comprising glufosinate and imazethapyr to the locus, wherein glufosinate and imazethapyr is applied at least 28 days after the first application.

[0213] In another embodiment, the present invention provides a method of controlling weeds at a locus, the method comprising: [0214] (a) applying a combination comprising glyphosate and triclopyr to the locus; and [0215] (b) applying a combination comprising L-glufosinate and imazethapyr to the locus, wherein L-glufosinate and imazethapyr is applied at least 7 days after the first application.

[0216] In an embodiment, the glyphosate herbicide is applied at a rate of about 3 L/ha along with triclopyr at a rate of 1.5 L/ha.

[0217] Generally single application of glyphosate herbicide at a rate of about 3 L/ha along with triclopyr at a rate of 1.5 L/ha provides less than 60% control of target weed.

[0218] In an embodiment, application of glyphosate herbicide at a rate of about 3 L/ha along with triclopyr at a rate of 1.5 L/ha and sequential application of glufosinate and glufosinate combinations provides more than 60% control of target weed. Typically, the present method of sequential application provides 70% control or 75% control or 80% control or 85% control or 90% control or 95% control of weed.

[0219] In an embodiment, the glyphosate herbicide is applied at about 3 L/ha along with triclopyr at a rate of 1.5 L/ha followed by an application of glufosinate at a rate of about 2 L/ha.

[0220] In an embodiment, the glyphosate herbicide is applied at about 3 L/ha along with triclopyr at a rate of 1.5 L/ha followed by an application of L-glufosinate at a rate of about 2 L/ha.

[0221] In an embodiment, the glyphosate herbicide is applied at about 3 L/ha along with triclopyr at a rate of 1.5 L/ha followed by an application of glufosinate and flumioxazin at a rate of about 1 L/ha.

[0222] In an embodiment, the glyphosate herbicide is applied at about 3 L/ha along with triclopyr at a rate of 1.5 L/ha followed by an application of L-glufosinate and flumioxazin at a rate of about 1 L/ha.

[0223] In an embodiment, the glyphosate herbicide is applied at a rate of about 3 L/ha along with triclopyr at 1.5 L/ha followed by an application of glufosinate, imazethapyr and carfentrazone at a rate of about 1.25 L/ha.

[0224] In an embodiment, the glyphosate herbicide is applied at a rate of about 3 L/ha along with triclopyr at 1.5 L/ha followed by an application of L-glufosinate, imazethapyr and carfentrazone at a rate of about 1.25 L/ha.

[0225] In an embodiment, the glyphosate herbicide is applied at about 3 L/ha along with triclopyr at a rate of 1.5 L/ha followed by an application of glufosinate and imazethapyr at a rate of about 1 L/ha.

[0226] In an embodiment, the glyphosate herbicide is applied at about 3 L/ha along with triclopyr at a rate of 1.5 L/ha followed by an application of L-glufosinate and imazethapyr at a rate of about 1 L/ha.

[0227] In an embodiment, the glyphosate herbicide is applied at about 3 L/ha along with triclopyr at a rate of 1.5 L/ha followed by an application of glufosinate and carfentrazone at a rate of about 1 L/ha.

[0228] In an embodiment, the glyphosate herbicide is applied at about 3 L/ha along with triclopyr at a rate of 1.5 L/ha followed by an application of L-glufosinate and carfentrazone at a rate of about 1 L/ha.

[0229] In another aspect the present invention provides a method of controlling herbicide resistant weeds such that, the method can be applied at pre or post emergence of weeds, thereby providing resistance management and complete control of the weeds.

[0230] The present invention thus provides a method for management of Spermacoce verticillate resistant to glyphosate and auxin herbicides, said method comprising sequentially applying the combinations of herbicides.

[0231] The present method can be used to control other tolerant or resistant weeds to glyphosate and 2,4-D, such as Conyza spp., Ipomeas spp, Spermacoce latifolia.

[0232] In another aspect the method of present invention provides effective controlling in herbicide resistant weeds such that, the method can be applied at pre or post emergence of weeds, thereby providing resistance management and complete control of the weeds.

[0233] In an aspect, the compositions of the present invention may be presented in the form of a multi-pack herbicidal product or as a kit-of-parts for treatment of targeted weeds.

[0234] In an embodiment, the present invention provides a kit-of-parts comprising a first component of a combination of glyphosate and triclopyr, and a second component of a combination of glufosinate and/or one or more herbicides selected from flumioxazin, imazethapyr, metolachlor and carfentrazone.

[0235] The kit-of-parts effectively controls herbicide resistant Spermacoce verticillate weeds.

[0236] The present invention therefore provides a method of controlling weeds thereby providing resistance management and synergistic control of the weeds. The present invention also provides an adequate method to achieve complete control of weeds, improving yields, while targeting resistance management.

[0237] The invention will now be described in more details with reference to the following examples. While the foregoing written description of the invention enables one of ordinary skill to make and use what is considered presently to be the best mode thereof, those of ordinary skill will understand and appreciate the existence of variations, combinations, and equivalents of the specific embodiment, method, and examples herein. The invention should therefore not be limited by the above described embodiment, method, and following examples, but by all embodiments and methods within the scope and spirit of the invention.

EXAMPLES

[0238] Trial Data for Evaluating Management of Spermacoce verticillate Weed

Example 1

[0239] Trials were carried out for evaluating the effect of herbicidal combinations on target weeds of Spermacoce verticillate resistant to herbicides belonging to Group 4 and Group 9. The evaluation for the damage on undesired weeds caused by the present compositions was carried out using a scale from 0 to 100%, compared to the untreated control plants. Here, 0 means no damage and 100 means complete destruction of weed. In the following experiments, the herbicidal activity for the herbicidal combinations was assessed till 28 days after treatment (application A).

[0240] In this trial, time T was application time, application A was performed first and at 15 days, application B was performed out in treatments with sequential application. The tested herbicides were sourced from the formulations readily available.

TABLE-US-00001 TABLE 1 Treatment Conc. Conc. active active First ingredient Rate Second ingredient Rates No. application (A) g/ha kg-L/ha application (B) g/ha kg-L/ha 1. Control 2. Glyphosate 1300 3 3. Triclopyr 720 1.5 4. Glyphosate + 1940 3.0 Triclopyr (A) 720 1.5 5. Glyphosate + 1940 3.0 Glufosinate 560 2 Triclopyr (A) 720 1.5 ammonium (B) 6. Glyphosate + 1940 3.0 Glufosinate 500 1.25 Triclopyr (A) 720 1.5 ammonium + 50 Flumioxazin (B) 7. Glyphosate + 1940 3.0 Glufosinate 340 1 Triclopyr (A) 720 1.5 ammonium + 85 imazethapyr + 85 carfentrazone (B)

[0241] The % weed control was observed at 14 days after the sequential application, i.e. 14 DA-B. The results were tabulated as hereunder:

TABLE-US-00002 TABLE 2 Treatment % weed control No. Treatment at 14 DA-B 1 Control 0 2. Glyphosate (A) 22 3. Triclopyr (A) 42.5 4 Glyphosate + 60 Triclopyr (A) 5 Glyphosate + Glufosinate 98.5 Triclopyr (A) ammonium (B) 6 Glyphosate + Glufosinate 94 Triclopyr (A) ammonium + Flumioxazin (B) 7 Glyphosate + Glufosinate 92 Triclopyr (A) ammonium + imazethapyr + carfentrazone (B)

[0242] The application of glyphosate and triclopyr, both individually and in combination do not efficiently control S. verticillate weeds. It was observed that S. verticillate was effectively controlled when sequential application of glufosinate, glufosinate+imazethapyr+carfentrazone, glufosinate+flumioxazin was used, after the application of glyphosate+triclopyr at 15 DA-A.

[0243] A single application (without a sequential application) the weed control was insufficient (about 60% and was not satisfactory).

[0244] Therefore, it was concluded that the present method causes an unexpected synergistic control of resistant Spermacoce verticillate weeds.

Example 2

[0245] Trials were carried out for evaluating the effect of herbicidal combinations on target weeds of Spermacoce verticillate resistant to herbicides belonging to Group 4 and Group 9, specifically, glyphosate and growth regulating herbicides. The evaluation for the damage on undesired weeds caused by the present compositions was carried out using a scale from 0 to 100%, compared to the untreated control plants. Here, 0 means no damage and 100 means complete destruction of weed. In the following experiments, the herbicidal activity for the herbicidal combinations was assessed till 42 days after the first application (A).

[0246] In this trial, time T was application time, application A was performed first and 15 days after this, application B was performed out in treatments with sequential application. The tested herbicides were sourced from the formulations readily available.

TABLE-US-00003 TABLE 3 Treatment Conc. Conc. active active First ingredient Rate Second ingredient Rates No. application (A) g/ha kg-L/ha application (B) g/ha kg-L/ha 1. Control 2. Glyphosate + 1440 3.0 Triclopyr (A) 720 1.5 3. Glyphosate + 1440 3.0 Glufosinate 560 2 Triclopyr (A) 720 1.5 ammonium (B) 4. Glyphosate + 1440 3.0 Glufosinate 400 1 Triclopyr (A) 720 1.5 ammonium + 100 imazethapyr + 10 carfentrazone (B) 5. Glyphosate + 1440 3.0 Glufosinate 500 1.25 Triclopyr (A) 720 1.5 ammonium + 50 Flumioxazin (B) 6. Glyphosate + 1440 3.0 Glufosinate 448 3.5 Triclopyr (A) 720 1.5 ammonium + 1050 Metolachlor (B)

[0247] The % weed control was observed 42 days after the first application, i.e. 42 DA-A. The results were tabulated as hereunder:

TABLE-US-00004 TABLE 4 Treatment % weed control No. Treatment at 42 DA-A 1 Control 0 2. Glyphosate + 30 Triclopyr (A) 3 Glyphosate + Glufosinate 71.3 Triclopyr (A) ammonium (B) 4 Glyphosate + Glufosinate 72.5 Triclopyr (A) ammonium + imazethapyr + carfentrazone (B) 5 Glyphosate + Glufosinate 73.8 Triclopyr (A) ammonium + Flumioxazin (B) 6 Glyphosate + Glufosinate 68.8 Triclopyr (A) ammonium + Metolachlor (B)

[0248] It was observed that S. verticillate was effectively controlled when a sequential application of glufosinate, glufosinate+imazethapyr+carfentrazone, glufosinate+flumioxazin and glufosinate+Metolachlor was used, after the application of glyphosate+triclopyr at 15 DA-A.