PREPARATION METHOD AND USE METHOD OF TEA DREG BEING DIRECTLY USED AS ADDITIVE THAT PROMOTES HEALTHY GROWTH OF ANIMAL

Abstract

Hormesis model (i.e. a toxicant excitation effect or a low dose promoting effect) is firstly used to study the relationship between the amount and efficacy of tea seed meal in feed industry. The amount of the tea seed meal as feed additive for the low dose promoting effect is determined to be 0.25-4 kg per ton of complete feed in the monogastric animal feed. The use method and the amount of the tea seed meal as feed additive can improve animal growth performance, such as increase daily weight gain, improve feed conversion ratio and decrease mortality.

Claims

1-10. (canceled)

11. A method comprising administering a feed additive to a monogastric animal, wherein the feed additive comprises tea seed meal.

12. The method according to claim 11, wherein the tea seed meal is administered at the amount of 0.25-4 kg per ton of complete feed.

13. The method according to claim 11, wherein the tea seed meal is administered at the amount of 0.5-2 kg per ton of complete feed.

14. The method according to claim 11, wherein the tea seed meal is administered at the amount of 0.8-1 kg per ton of complete feed.

15. The method according to claim 11, wherein the monogastric animal comprises a bird, a pig, a dog, or a cat and the bird comprises a chickens, a duck, a turkey, a quail, or a pigeon.

16. The method according to claim 11, wherein: the tea seed meal comprises a byproduct of oil-extracted Camellia plant seeds; the tea seed meal is not subjected to any processing for detoxification purposes; and the Camellia plant seeds comprises Camellia oleifera seeds, tea leaf seeds, or a combination thereof.

17. The method according to claim 11, wherein the tea seed meal comprises tea saponin at the amount of 8-25 wt %.

18. The method according to claim 11, wherein the tea seed meal comprises tannin at the amount of 0.5-7 wt %.

19. The method according to claim 11, wherein the active ingredients of the Tea seed meal comprises tea saponin, tannin, tea polyphenol, flavonoid compounds, and ceramide.

20. The method according to claim 11, wherein the tea seed meal is in a grass, grassless, powder, granular, or cake-like form.

21. A method comprising administering a feed additive to an animal, wherein the feed additive comprises tea seed meal.

22. The method according to claim 21, wherein the tea seed meal is administered at the amount of 0.25-4 kg per ton of complete feed.

23. The method according to claim 21, wherein the tea seed meal is administered at the amount of 0.5-2 kg per ton of complete feed.

24. The method according to claim 21, wherein the tea seed meal is administered at the amount of 0.8-1 kg per ton of complete feed.

Description

DETAILED DESCRIPTION

[0047] A large number of literature, combined with working experience, several points of the invention are summarized as follows:

[0048] First Part, there are four possible main reasons of technical prejudice against Tea seed meal in the feed industry. [0049] 1. The Tea seed meal is generally considered to be the agricultural and sideline products with certain toxicity and is not suitable for being directly used in the feed; the understanding is based on the existing experience and animal test cases, and indeed, the understanding and understanding of the so-called toxic are general and fuzzy, and still stay in toxic and non-toxic traditional cognitive stages. According to the traditional poison risk model, the toxicity of the Tea seed meal is indeed possible to belong to a threshold model instead of a linear non-threshold model. Because the feed industry technician has little knowledge and accurate cognition on the Hormesis model, scientific judgment cannot be carried out; therefore, it is difficult to identify the toxicity model of the Tea seed meal from another angle (such as improving the growth performance of the animals), and can also belong to the Hormesis model, namely the dual-phase dose effect model. [0050] 2. In addition, the Tea seed meal is generally considered to be toxic and cannot be directly used by people in the feed industry; and the main reason is that tea seed meal is used as feed raw materials. The deep analysis is as follows: 1). The Tea seed meal, the soybean meal, the rapeseed meal and the like are all by-products of an edible oil plant; 2) All meal byproduct of plant oil contain high level of crude proteins, 3) The price of the Tea seed meal is cheaper, only about 2000 RMB per ton, and is easy to be purchased; therefore, a person skilled in the feed industry is easy to treat it as a feed raw material, and once it is used as a feed raw material, the dosage of the starting point is 1-3%, that is, 10-30 kg of Tea seed meal is added per ton feed, and at least 5 kg of per ton feed (such as 0.5%, 0.75 and 1%); and if Tea seed meal as feed raw materials, which is not detoxicated in advance, there is a significant side effect when Tea seed meal used as feed raw material, such as significant reduction in feed intake, slower growth of animal, that is, the commonly-known Tea seed meal is toxic, and meanings Tea seed meal cannot be used in the feed directly at all. [0051] 3. Tea seed meal cannot be directly used in animal feed, but a person skilled in the art tends to remember that the price of the Tea seed meal is cheap, the content of crude protein is high, and the Tea seed meal is easy to be purchased. Therefore, a person skilled mostly try to make Tea seed meal detoxified or attenuated (because of the anti-nutritional factors such as tea saponin, tannin and the like, e.g., remove tea saponin or fermentation detoxified), then the detoxified Tea seed meal can be used to instead of other more expensive feed raw materials (like soybean meal) in feed, so that the cost is reduced. The detoxified Tea seed meal used as a feed raw material and the detoxified Tea seed meal can be added in the feed about 5-10%, and allowed more amounts added in the feed, the animal test effect of this method can be accepted, the cost can be reduced, and therefore, the method has a certain market. In particular, after tea saponin (high added value) is extracted, residual (detoxification) is carried out, The sale price of the detoxified Tea seed meal is still approximately 2000 RMB per ton. and The price gap between Tea seed meal and detoxificated Tea seed meal is small and not obvious; the factory of Tea seed meal has more driving force and enthusiasm to extract tea saponin with high added value, so that the Tea seed meal becomes two types of products, namely tea saponin and detoxificated Tea seed meal, the economic benefit is obviously increased, the manufacturer disputes and emulates, a large amount of detoxified Tea seed meal (i.e., the residue after tea saponin extracted) is marketed and used as a feed raw material, the problem of Tea seed meal used in feed is solved in a certain extent, and the problem of Tea seed meal used in feed is no longer urgent. [0052] 4. In addition, from the view of price, the Tea seed meal is very cheap, is only 2000 RMB per ton, which is cheaper than the raw materials, such as soybean meal and the like; therefore, the Tea seed meal is classified into raw materials, such as soybean meal, rapeseed meal and the like; in fact, on the large premise, the functional positioning of the Tea seed meal is wrong, and the Tea seed meal should not be taken/classified as a feed raw material; The function position and classification of Tea seed meal is wrong, and the usage method of Tea seed meal is naturally prone to one error.

[0053] Second Part, a three-pronged analysis reveals comprehensively the prejudice against the Tea seed meal in the feed industry: [0054] 1. A deep and careful analyses of the Tea seed meal determines a plurality of unique active ingredient characteristics contained in the Tea seed meal, corresponding effective content characteristics and efficacy characteristics (such as sterilization, bacteriostasis, anti-inflammation, oxidation resistance, immunity improvement and the like.). The three features are completely more consistent with the main technical features of the feed additive; although according to several surface features of the Tea seed meal (meal, by-products of the edible oil plant, more crude protein is contained, the price is also low.) The Tea seed meal is classified as a feed raw material class and it is also reasonable; however, comprehensive evaluation is carried out, and the functional feed additive class is preferred according to the main salient characteristics and the comparison advantages of the Tea seed meal, instead of the feed raw material class, and is more suitable. Tea seed meal should not be fuzzy positioning and wrong positioning. [0055] 2. From the view of Inner essence and active ingredients content of The Tea seed meal, which has the effects of sterilizing and inhibiting bacteria, diminishing inflammation, killing viruses, resisting oxidation and improving immunity. From the view of practical application effect of the patent, the Tea seed meal does have the effect of promoting the growth of livestock and poultry, increasing the daily gain, improving the FCR, and reducing the death rate of the disease; therefore, the Tea seed meal is a remarkable and plant source feed additive, can partially replace or completely replace antibiotic growth promoter (AGP) and other planting feed additive in the market, and the Tea seed meal is a real cheap and green feed additive. [0056] 3. A deeply understanding of the toxic excitement effect (Hormesis) is needed. After the Hormesis model is analyzed, after the main action mechanism of the active ingredients in the Tea seed meal is analyzed, the majority of tea saponin is not absorbed by the intestinal tract, mainly acts on the surface of the cell membrane, enhances the permeability of the cell membrane, and promotes the absorption of nutrient substances. After actual working experience, it is confirmed that the toxicity model of the Tea seed meal in animal feed is a Hormesis model, which is also referred to as a biphasic dose effect model, that is, when a high dose (as in the broilers trial reported by Sun yanfa), namely the use amount of the Tea seed meal in feed is 0.5%, 0.75%, 1%) is harmful to the organism/animal, and the low dose (as in the embodiment of the present patent) is 0.025%-0.4% is beneficial. The relationship of effect and amount of such poison material is also referred to as a U-type curve or a low dose promotion effect mode. The invention needs to protect the claim that Tea seed meal as feed additive and the dosage range of the Tea seed meal in feed, low-dose promotion Effect, that is, the range of the Lowest effective dose of the theoretical Lowest effective dose (NOAEL) to the non-visible harmful dose (NOAEL), which is equivalent to namely dosage range is 250 grams to 4 kilograms of the Tea seed meal added/supplemented per ton animal feed in the patent.

[0057] Third part: ten points of the principles of the invention and the key points of the invention are summarized as follows: [0058] 1. Three technical features are summarized from the comparison advantages of Tea seed meal (common Tea seed meal containing 15% of tea saponin, and about 2% of tannin), namely rich and diverse active ingredient characteristics, effective content characteristics and actual efficacy characteristics, and it is determined that the Tea seed meal should be preferred as a functional feed additive, and the Tea seed meal should not be attributed to feed raw materials. [0059] 2. The tea seed meal is a treasure, and contains various natural active ingredients, such as tea saponin, and the tea polyphenol comprises tannin (proanthocyanidins)) flavone, ceramide, caffeine alkaloid and the like, and is rich in variety and high in content; the unique active components are naturally gathered together and possibly have the synergistic effect for animal natural growth, and the Tea seed meal do not need to be extracted or extracted with an organic solvent at all; the active ingredients in Tea seed meal are difficult to completely extract through a solvent; some of the active ingredients are reduced or lost completely in the extraction process. The Tea seed meal is directly added to the feed for use, all activity and nutrition are achieved, no ingredient loss or activity is reduced, all the effective components are fully utilized, and the unique biological activity is exerted. [0060] 3. According to the invention, it is believed that the main active ingredient is tea saponin in the Tea seed meal, tea saponin is excellent in non-ionic surface active agent, has a hydrophilic group and an oleophilic group, and has good stability; Tea saponin does not need to be absorbed into intestinal cells to play a role; (1) The tea saponin plays an active role in the intestinal cavity, has a very strong and stable emulsification effect and can promote digestion and absorption of grease; (2) Tea saponin can be combined with cholesterol on intestinal cell mucosa, activate the cell lipid raft, enhance the permeability of the cell membrane and promote the absorption of intestinal cells to nutrient substances; and (3) Tea saponin can activate intestinal immune related cells, particularly APC cells, macrophages T cells and the like, and enhance the immune response reaction. [0061] 4. Due to the fact that the tea saponin in the Tea seed meal is taken orally, the tea saponin in the Tea seed meal is not absorbed by the intestinal tract, and the tea saponin is discharged out of the body; therefore, after the corresponding sufficient effective dose of tea saponin is reached, the tea saponin is additionally added without a plurality of benefits, and a certain side effect is caused, such as the feed intake of the animal is decreased, and the intestinal cell mucosa is damaged; therefore, control the use amount of Tea seed meal in animal feed is very important. [0062] 5. The following is to accurately recognize and scientifically apply the poison excitation effect model (dual-phase dose effect model or U-shaped mode) of the Tea seed meal In the case of high dose, which is harmful to organisms, while low dose is beneficial to organisms; in combination with animal trial results of related Tea seed meal, scientific analysis, comprehensive research and judgment: the lowest effective dose (LOEL, Lowest Observed Effect Level), the Maximum Response Level (MRL), No observed effect level (NOEL) and the proportional relationship between the three dosage values. The average of NOAEL values is about 10-20 times the LOEL value; the Maximum Response Level average is about one-fifth of the NOAEL value. [0063] 6. The method described here comprises the following steps: comprehensively considering the content of active ingredients in the Tea seed meal, the use amount of the Tea seed meal in the feed, and the dialectical relationship among the use effects; scientifically and reasonably formulating the quality standard of the Tea seed meal product, and standardizing the usage amount and efficacy of the recommended usage in the feed. [0064] 7. Different tea seed varieties, different origin sources, different processing technologies and various active ingredients in the Tea seed meal are fluctuation and difference. In order to avoid the high level of effective component concentration in the finished product due to fluctuation of the raw material quality, scientific optimization matching of high and low raw materials can be carried out on the basis of detecting the quality standard of the product after each batch of raw materials is detected, so that the content of effective active ingredients in the semi-finished product is stable, the quality of the finished product is reliable, the carrier/excipient can also be selected for proportioning, and the standardization of the product is facilitated. [0065] 8. The key points of the invention are the prejudice that the technical bias of tea seed meal is corrected, that is, the Tea seed meal is toxic and cannot be directly used in the feed; meanwhile, the Tea seed meal is accurately positioned as a functional feed additive, and the protein in the Tea seed meal is not taken as a feed raw material; in the feed industry, a poison excitation effect model (double-phase dose effect model) is adopted for the Tea seed meal for the first time). The Tea seed meal can be accurately analyzed, and the relationship between the effective component content (such as the tea saponin content in the common Tea seed meal is about 15%), the different usage doses and the actual functional effect are accurately analyzed; the range of low-dosage use of the Tea seed meal in the complete feed for livestock and poultry is preliminarily determined to be 250 grams to 4 kilograms per ton feed. [0066] 9. According to the invention, the standard content of effective components in the Tea seed meal is scientifically set, the use amount is accurately and reasonably recommended, and the effect can be expected to be realized; the use safety of the Tea seed meal is safe, convenient and efficient, and all the effects of the Tea seed meal are fully exerted to a great extent; meanwhile, the product quality is kept stable and does not fluctuate; meanwhile, the production of the product can be standardized, specialized, large-scale and industrialized. [0067] 10. The Tea seed meal functional feed additive has extremely high practicability, and is very beneficial to realizing the upgrading and updating of the Tea seed meal industry; the Tea seed meal functional feed additive is accurately positioned, and the added value of the Tea seed meal functional feed additive is rapidly improved; the later Tea seed meal direct food additive, the Tea seed meal health product and the like are greatly facilitated; the practical application experience is laid for making a strong tea seed meal industry chain, and the practical application experience is laid for making the strong Tea seed meal industry chain.

[0068] Supplemental Description: A plan after patent application file publication or patent granted authorization.

[0069] It is intended to apply for product registration or apply for listed in approved feed additives catalogues after the patent got some protection. The patent of the invention is to push and promote the progress of the entire science and technology, and has certain advantages and leading properties. The product, tea seed meal (including the technical scheme or the use scheme) of the invention is not approved as feed additive by official or not in the allowed directory, and it can be completely understood.

[0070] Under the premise of certain rights protection, various materials and reports will be prepared actively and submit files to administrative department for allow the use of tea seed meal as feed additive or obtain the license of feed additive by administrative permissions; and then actual investment is carried out (authorized production and or sales).

[0071] Followings are 6 technical advantages and technical effects of the invention. [0072] 1. The Tea seed meal is easily sourced, and can be produced rapidly; meanwhile, by-products of the vast tea farmers are efficiently utilized, the demand of tea seed meal in feed industry is larger, the Tea seed meal can be better sold to feed mill, meanwhile, the price of tea seed meal can be increased, and the income of tea growers can be improved. [0073] 2. The processing cost of the Tea seed meal powder is low or the zero cost. Some manufacturers of tea seed meal themselves produce the tea seed meal fine powder, only needs to be simply replaced into a feed grade label, and can be directly sold, and the technical scheme provided by the invention has almost zero cost. If the Tea seed cake/meal or the coarse grain Tea seed meal is processed into the tea seed meal fine powder, the production method is simple, zero emission in the production process is realized, and pollution is avoided; the Tea seed meal is fully utilized; only the common pulverizer is required to be crushed and cost of processed is low (about 80-200 RMB of crushing cost per ton), and almost no threshold, and is easy to learn and easy to popularize. The Tea seed meal is sold and used according to the feed additive in the feed, that is, the application of the Tea seed meal is expanded or changed, and the user/customer number is violently increased; the Tea seed meal directly as feed additive, which is an ingenious innovation; therefore, the amount of the Tea seed meal direct sold to the feed industry is increased, and the economic benefit of the manufacturer is obviously increased. [0074] 3. The method is convenient to use and low in use cost. The Tea seed meal product is directly added in the feed, does not need to be detoxified, can be directly low in dosage for a long time (e g, 1-2 kg per ton), is simple and convenient, is low in use cost (about 2-4 RMB per ton of cost), and has a remarkable disease-resistant growth-promoting effect and an exact effect. At the same time, other planting feed additives with more expensive price can be partially or completely replaced, so that the cost can be obviously saved; meanwhile, the use of growth-promoting antibiotics can be partially or completely replaced, and the residue and the pollution of antibiotics are reduced. [0075] 4. The Tea seed meal product can be widely used in animal feed, can be used for a long time, and has no pollution to the environment. [0076] 5. The Tea seed meal product has the effects of resisting bacteria, preventing diseases and promoting growth, and also has the effects of diminishing inflammation, resisting oxidation and enhancing the immunity of animals; the Tea seed meal product is purely natural and pollution-free, and is an extremely-promising alternative growth-promoting green product. According to the technical scheme of the invention, the technical scheme of the invention has the benefits that the multi-party benefits, the Tea seed meal manufacturer or the customer (feedmill or farm of raising animal) are easy to adopt, the technical scheme of the invention will bring many benefit for the multiple parties, therefore, large-area popularization and common use are easily obtained, and good economic benefits, ecological benefits and social benefits are achieved. [0077] 6. The invention scientifically and simply solves the technical problem of how to use Tea seed meal in feed. In the range of the recommended dosage of Tea seed meal as feed additive, tea seed meal can promote growth in livestock and poultry, the ADG (average daily gain) increased, the FCR improved, and the disease death rate reduced; meanwhile, the toxic and side effects of the Tea seed meal on livestock and poultry do not need to be worried.

[0078] The specific technical scheme of the invention is as follows (a preparation method and a use method):

[0079] The preparation method of the Tea seed meal product comprises the following steps: [0080] 1. The preparation method comprises the following steps: purchasing various kinds of Tea seed meal (including cakes, pieces, and particles), removing foreign matter, drying in the sun or by drying machine. [0081] 2. The preparation method comprises the following steps: carrying out mechanical crushing on the raw materials at room temperature, and crushing the raw materials into about 80-150 meshes; [0082] 3. The content of tea saponin and the content of tannin (the tea polyphenol) are detected according to the ethanol extraction weight method and recorded; different quality standards of the product (such as, no less than 15% of tea saponin and no less than 1.5% of tannin are set as standard grade products; the content of tea saponin is 10-15%, the tannin content is less than 1.5%, and the tea saponin content is a common grade product; if the tea saponin content is 10% or less, which be regarded as a secondary product). [0083] 4. Preparation method comprises the following steps: detecting items of other conventional components (such as crude protein and ash, etc.) and health indexes in a product, and recording the items. [0084] 5. After the reexamination is qualified, the label with the corresponding grade is pasted, and then packaged.

[0085] The application method of the Tea seed meal product in feed comprises the following steps:

[0086] The Tea seed meal standard-grade product (tea saponin content is not less than 15%, the tannin content is not less than 1.5%) is taken as a precondition and a foundation.

[0087] According to the biphasic dose effect model of the Tea seed meal Hormesis theory, the dosage range in the low-dose promotion effect of the Tea seed meal is the lowest effective dose (LOEL) from the theory to no visible harmful dose (NOAEL), and the NOAEL value is 10-20 times the LOEL value; the Optimal Dose is one-fifth of the NOAEL value.

[0088] In combination with the trial data in the embodiments of the invention, the use amount of the Tea seed meal is added from 250 grams to 4 kilograms of different gradient amounts per ton feed; and 250 grams (see the same LOEL value) to 4 kg (equal to NOAEL value), and the numerical proportion relationship of the two is close to 20 times; the numerical proportion relation is basically consistent with the Hormesis theory; meanwhile, the optimal dosage of the Tea seed meal confirmed by the trial is 1 kg, and the optimal dosage of the Tea seed meal is similar to that of the theoretical optimal dose (NOAEL 5), i.e., 4 kg/5=0.8 kg.

[0089] Therefore, the optimal dosage of the Tea seed meal standard product is 0.8-1 kg per ton of feed and the growth effect on livestock and poultry is best. If the Tea seed meal is used for preventing and treating the disease at a certain stage, the short-term dosage of tea seed meal (only for one to two weeks) is added, the suggested dosage of tea seed meal in feed is 2-4 kg per ton feed.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0090] The following example explains the present invention more concretely, but do not limit the range of the present invention.

Example 1. Tea Seed Meal Product Preparation Method

[0091] The preparation method comprises the following steps of:

[0092] 1.) Purchasing 20 kg Tea seed meal produced in Zhejiang province, China.

[0093] 2.) Carrying out mechanical crushing to obtain fine powder of about 120 meshes.

[0094] 3.) The tea saponin and tannin content in above tea seed meal are detected, the tea saponin is 15.6%, and the tannin content is 1.9%.

[0095] 4.) Labeling corresponding labels and marking detection/analysis values for later use.

Example 2. Broiler Growth Performance Trial

[0096] Trial design: selecting 1 day age AA broiler 900 with basically consistent size; randomly dividing into six groups, namely one control group and five test groups; each group of 150 birds. Control group (T0) The basic daily ration is fed; and the trial No. 1, 2, 3, 4 and 5 groups are respectively fed with the basic daily ration added the Tea seed meal product got in above embodiment 1, namely 250 g, 500 g, 1 kg, 2 kg, 4 kg/ton, referred to as T 1, T 2, T 3, T 4 and T 5 groups. The broiler chicken adopts a thick cushion material for flat breeding; the day lighting, the temperature and the illumination condition are basically consistent; all the broilers are same feeding and drinking water; and conventional management work is carried out during feeding. The basic daily ration is prepared by reference to the nutrition standard of the AA commodity broiler chicken, and is the corn soybean meal type daily ration and the crude protein 19.5 percent. The Trial period is 42 days, and the Trial results are as follows:

TABLE-US-00001 TABLE NO. 1 T0 T1 T2 T3 T4 T5 ADG 50.62 51.19 52.43 53.76 51.27 50.63 (grams/day) FCR 1.830 1.783 1.745 1.742 1.757 1.841 Number of 9 6 5 3 0 0 Death

[0097] The Trial results show that the ADG (daily weight gain) and the FCR of the T3 group reach a best level, that is, when the dosage of the Tea seed meal is 1 kg/ton, the effect is best, and the ADG is increased by 6.20% when compared with that of the control group; when the dosage reaches 4 kg/ton, the ADG and the FCR do not change obviously; however, as the dosage of the Tea seed meal is increased, the death number of the chickens is reduced.

Example 3. Pig Growth Performance Trial

[0098] The Trial design is as follows: 120-head healthy piglets are selected, the average body weight is about 15 kg, and piglets are randomly divided into six groups, each group of 20 head piglets. The control group (T0) is fed with basic daily ration; and No. 1, 2, 3, 4, and 5 groups are respectively fed with the basic daily ration added 250 g, 500 g, 1 kg, 2 kg, 4 kg/ton of Tea Seed Meal got in above embodiment 1, referred to as T1, T2, T3, T4 and T5. All trial pigs are same feed and drinking water, fed according to the conventional method, and the trial period is 30 days. The trial results are as follows:

TABLE-US-00002 TABLE NO. 2 T0 T1 T2 T3 T4 T5 ADG 510.3 514.8 529.4 536.7 519.4 503.8 (grams/day) FCR 2.058 2.061 1.953 1.864 1.892 2.045 number of 5 4 2 1 0 0 diarrhea

[0099] The trial results show that the ADG (Daily Weight Gain) and the FCR of the T3 group reach the best effect, and the ADG is increased by 5.06% when compared with the control group; when the dosage is above 4 kg/ton, the ADG and FCR are both poor, and the daily gain weight and the FCR have no significant difference when compared with control group (T0). However, as the dosage of the Tea seed meal is increased, the number of pig diarrhea is reduced.

[0100] The Trial is summarized as follows: when the dosage of the Tea seed meal in the feed is 0.25-1 kg, the daily weight gain and the FCR are improved; When the dosage is increased to 4 kg, although the daily weight gain and the FCR are no obviously improved when compared with the control group (T0), the morbidity is obviously reduced, and this can be due to the fact that active substances such as tea saponin and tannin, flavone and the like contained in the Tea seed meal are increased along with the dosage. The sterilization ability is obviously enhanced, and therefore, the resistance of the animal is obviously enhanced, and the animal is not prone to sick or death. The use amount of the Tea seed meal in feed is preliminarily determined to be within 0.25 to 4 kilograms per ton through the above results, and the usage method is generally beneficial; the usage method also conforms to the low-dose promotion effect model of the Hormesis theory; and Tea seed meal is recommended to be added in feed for a long time, and the optimal dosage is 1 kg per ton. For preventing disease from occurring at a certain period, the usage amount of tea seed meal can be adjusted to 2-3 kg per ton in a short period feeding (such as one to two weeks). If the disease occurs, the addition of 4 kg of tea seed meal can be attempted, and this usage method has positive effect on preventing and treating diseases.

[0101] Meanwhile, the production method is simple and easy to operate; Some powdery products of tea seed meal do not need to be processed and can be directly used; The usage and production method disclosed by the invention has zero emission and full utilization, and belongs to green and safe health products; The usage method has the advantages of environment friendliness, ecological friendliness, no pollution discharge, no harmful residue during use, and sustainable development; The feed mill enterprises and farm both get benefit from this invention as Tea seed meal as good and cheap green feed additive, the growth-promoting and disease-prevention effect of Tea seed meal is exactly confirmed. The usage method is easy to popularize, and the advantages of being easy to popularize, capable of directly benefiting for tea growers and tea oil plants, also benefiting for vast farmers, turning waste into wealth, and being beneficial to human being.

[0102] The above-described embodiments are merely exemplary embodiments of the invention, and are used to explain the technical solutions and technical effects of the present invention and are not intended to cover all possible embodiments, nor to limit the invention.

[0103] The usage method disclosed by the invention has the advantages that technical prejudice of Tea seed meal in the feed industry are corrected, the Hormesis model is adopted, namely a low-dose promotion effect is adopted.

[0104] The belief of the tea seed meal is toxic and cannot be used in feed was thoroughly corrected, and a science-based concept is built that Tea seed meal as feed additive is beneficial at low dosage (the preferred dosage is 0.8-1 kg per ton feed). According to the invention of tea seed meal as feed additive, within the scope of the protection of the present invention (0.25 to 4 kg), any simple modifications and changes made to the present invention, such as addition of carrier dilution and the like, fall within the scope of the present invention protection.

[0105] At the same time, based on the embodiments of the invention and the direct obvious motivation, a person of ordinary skill in the art, on the premise of not making an inventive effort, defines the use dosage of the Tea seed meal as feed additive from 0.25 to 4 kilograms per ton of complete feed, and extends from the chicken and the pig feed to other single-stomach animal feed, such as ducks, turkeys, quails, birds and pigeons. The poultry includes corresponding meat poultry, laying-egg poultry and breeding birds, and pets (including dog and cats). In addition, other embodiments obtained by other equivalent or equivalent simple alternatives methods are all within the scope of protection of our invention.

[0106] All publications and patents cited in this specification are herein incorporated by reference as if each individual publication or patent were specifically and individually indicated to be incorporated by reference and are incorporated herein by reference to disclose and describe the methods and/or materials in connection with which the publications are cited. The citation of any publication is for its disclosure prior to the filing date and should not be construed as an admission that the present invention is not entitled to antedate such publication by virtue of prior invention. Further, the dates of publication provided may be different from the actual publication dates which may need to be independently confirmed. To the extent a definition of a term set out in a document incorporated herein by reference conflicts with the definition of a term explicitly defined herein, the definition set out herein controls.