COMPOUNDS INCLUDING A MUTANT KRAS SEQUENCE AND A LIPID AND USES THEREOF

Abstract

The invention features a compound including a mutant KRAS sequence and a lipid, where the mutant KRAS sequence is conjugated to the lipid by a linker, and (i) the linker includes one or more polyethylene glycol blocks, (ii) the lipid is 1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE), and (iii) the mutant KRAS sequence comprises or consists of the amino acid sequence selected from the group consisting of SEQ ID NOs:1-7 and 22-30. The invention features a composition including one or more compounds of the invention and a pharmaceutically acceptable carrier. The invention also features a method of treating a cancer in a human patient, the method including administering the composition to the patient. Further, the invention features a kit comprising the compound.

Claims

1. A compound comprising a mutant KRAS sequence and a lipid, wherein the mutant KRAS sequence is conjugated to the lipid by a linker, and (i) the linker comprises one or more polyethylene glycol blocks, (ii) the lipid is 1,2-distearoyi-sa-glycero-3-ohosphoethanolarnine (DSPE), and (iii) the mutant KRAS sequence consists of an amino acid sequence selected from the group consisting of CYKLVVVGADGVGKSALTI (SEQ ID NO:1), CYKLVVVGAVGVGKSALTI (SEQ ID NO:2), CYKLVVVGARGVGKSALTI (SEQ ID NO:3), CYKLVVVGAAGVGKSALTI (SEQ ID NO:4), CYKLVVVGASGVGKSALTI (SEQ ID NO:5), CYKLVVVGACGVGKSALTI (SEQ ID NO:6), CYKLVVVGATGVGKSALTI (SEQ ID NO:22), and CYKLVVVGAGDVGKSALTI (SEQ ID NO:7),

2. The compound of claim 1, wherein the mutant KRAS sequence consists of an amino acid sequence selected from the group consisting of YKLVVVGADGVGKSALTI (SEQ ID NO:23), YKLVVVGAVGVGKSALTI (SEQ ID NO:24), YKLVVVGARGVGKSALTI (SEQ ID NO:25), YKLVVVGAAGVGKSALTI (SEQ ID NO:26), YKLVVVGASGVGKSALTI (SEQ ID NO:27), YKLVVVGACGVGKSALTI (SEQ ID NO:28), YKLVVVGATGVGKSALTI (SEC) ID NO:29), and YKLVVVGAGDVGKSALTI (SEQ ID NO:30).

3. The compound of claim 1 or 2, wherein the mutant KRAS sequence, at its N-terminus, is conjugated to the linker through a cysteine-maleimide linkage.

4. The compound of claim 1 or 2, wherein linker comprises 48 repeat units of polyethylene glycol.

5. The compound of claim 1 or 2, wherein the mutant KRAS sequence, at its N-terminus, is conjugated to the following structure: ##STR00020##

6. A composition comprising one or more compounds of claim 1 or 2, and a pharmaceutically acceptable carrier.

7. The composition of claim 6, wherein the composition comprises (1) a compound comprising the amino add sequence YKLVVVGADGVOKSALTI (SEQ ID NO:23), (2) a compound comprising the amino add sequence YKLVVVGAVGVGKSALTI (SEQ ID NO:24), (3) a compound comprising the amino acid sequence YKLVVVGARGVGKSALTI (SEQ ID NO:25), (4) a compound comprising the amino add sequence YKLVVVGAAGVGKSALTI (SEQ ID NO:26), (5) a compound comprising the amino acid sequence YKLVVVGASGVGKSALTI (SEQ ID NO:27), (6) a compound comprising the amino acid sequence YKLVVVGACGVGKSALTI (SEQ ID NO:28) or a compound comprising the amino acid sequence YKLVVVGATGVGKSALTI (SEQ ID NO:29), and (7) a compound comprising the amino acid sequence YKLVVVGAGDVGKSALTI (SEQ ID NO:30).

8. The composition of claim 6, wherein the composition comprises (1) a compound comprising the amino acid sequence CYKLVVVGADGVGKSALTI (SEQ ID NO:1), (2) a compound comprising the amino acid sequence CYKLVVVGAVGVGKSALTI (SEQ ID NO:2), (3) a compound comprising the amino acid sequence GYKLVVVGARGVGKSALTI (SEQ ID NO:3), (4) a compound comprising the amino acid sequence CYKLVVVGAAGVGKSALTI (SEQ ID NO:4), (5) a compound comprising the amino acid sequence CYKLVVVGASGVGKSALTI (SEQ ID NO:5), (6) a compound comprising the amino acid sequence GYKLVVVGACGVGKSALTI (SEQ ID NO:6) or a compound comprising the amino acid sequence CYKLVVVGATGVGKSALTI (SEQ ID NO:22), and (7) a compound comprising the amino acid sequence GYKLVVVGAGDVGKSALTI (SEQ ID NO:7).

9. The composition of claim 6, wherein the composition further comprises a compound consisting of the nucleotide sequence 5-TCGTCGTTTTGTCGTTTTGTCGTT-3 (SEQ ID NO:8), which, at its 5 end, is bonded or linked by a linker to the following lipid: ##STR00021## or a salt thereof, wherein X is O or S.

10. The composition of claim 6, wherein the composition comprises 700 g of each compound.

11. The composition of claim 9, wherein the nucleotide sequence is bonded to the lipid.

12. A method of treating a cancer in a human patient, the method comprising administering the composition of claim 6 the patient.

13. The method of claim 12, wherein the method further comprises administering an adjuvant.

14. The method of claim 13, wherein the adjuvant comprises a CpG nucleotide sequence.

15. The method of claim 14, wherein the CpG nucleotide sequence comprises 5-TCGTCGTTTTGTCGTTTTGTCGTT-3 (SEQ ID NO: 8).

16. The method of claim 12, wherein 0.1 mg, 0.5 mg, or 2.5 mg of the adjuvant is administered.

17. The method of claim 12, wherein the method further comprises administering to the patient a compound consisting of the nucleotide sequence 5-TCGTCGTTTTGTCGTTTTGTCGTT-3 (SEQ ID NO:8), which, at its 5 end, is bonded or linked by a linker to the following lipid: ##STR00022## or a salt thereof, wherein X is O or S.

18. The method of claim 17, wherein the nucleotide sequence is bonded to the lipid.

19. The method of claim 17, wherein 0.1 mg, 0.5 mg, or 2.5 mg of the compound consisting of the nucleotide sequence 5-TCGTCGTTTTGTCGTTTTGTCGTT-3 (SEQ ID NO:8), which, at its 5 end, is bonded or linked by a linker to the following lipid: ##STR00023## or a salt thereof, wherein X is O or S, is administered.

20. The method of claim 12, wherein the cancer is a pancreatic cancer, a lung cancer, or a colorectal cancer.

21. The method of claim 12, wherein all internucleoside groups connecting the nucleosides in 5-TCGTCGTTTTGTCGTTTTGTCGTT-3 (SEQ ID NO:8) are phosphorothioates.

22. A kit comprising (i) a compound of claim 1 or 2, and (ii) a compound consisting of the nucleotide sequence 5-TCGTCGTTTTGTCGTTTTGTCGTT-3 (SEQ ID NO:8), which, at its 5 end, is bonded or linked by a linker to the following lipid: ##STR00024## or a salt thereof, wherein X is O or S.

Description

DESCRIPTION OF THE DRAWINGS

[0042] FIG. 1 shows that amphiphile-KRas (aKRas), in combination with amphiphile-CpG (aCpG), activated splenocytes, while soluble KRas, in combination with aCpG, and the untreated and soluble KRas and polyiC (pIC) control groups, did not.

[0043] FIG. 2 shows that both aKRas G12R and G12V, in combination with aCpG, activated splenocytes, while neither soluble KRas G12R nor G12V in combination with aCpG, and the untreated and soluble KRas and pIC control groups, did not.

[0044] FIG. 3 shows a KRas-specific CD8+T-cell response for mice immunized with the combination of aKRas G12D and aCpG, but not for soluble KRas G12D in combination with CpG or soluble wild-type KRas in combination with CpG.

[0045] FIG. 4 shows a KRas-specific CD8+T-cell response (9 mers) and CD8+ or CD4+T-cell response (18 mer) for mice immunized with the combination of aKRas G12D and aCpG, but not for soluble KRas G12D in combination with CpG or the untreated control.

[0046] FIG. 5 shows amphiphile-KRas (Amph-KRAS) and amphilpile-CpG (Amph-CpG) vaccines elicit superior endogenous T-cell responses compared to soluble KRas vaccines, as indicated by increased cytokine production by splenocytes in a cytokine bead array. Soluble KRAS G12D =YKLVVVGADGVGKSALTI (SEQ ID NO: 23); Amph-KRAS G12D =DSPE-amido-dPEG24-amido-dPEG24-CYKLVVVGADGVGKSALTI (SEQ ID NO: 1); KRAS G12D 18-mer =YKLVVVGADGVGKSALTI (SEQ ID NO:23); CBA=Cytokine bead array; IFN=interferon; IL=interleukin; KRAS =Kirsten Rat Sarcoma.

[0047] FIG. 6 shows the structure of the amphiphile-peptide G12D 4-21 (SEQ ID NO:1).

[0048] FIG. 7 shows the structure of amphiphile-CpG-7909. All sugars are deoxyribose. All internucleoside linkages are phosphorothioate. The structure is presented as the sodium salt.

DETAILED DESCRIPTION OF THE INVENTION

[0049] The invention provides compounds that can be used in therapeutic methods. The compounds of the invention each include a mutant KRAS sequence (e.g., any one of SEQ ID NOS:1-7 and 22; see Table 1, below, or any one of SEQ ID NOS:23-30, see Table 2, below) and a lipid (e.g., 1,2-distearoyi-sn-glycero-3-phosphoethanolarnine (DSPE)). These moieties (the mutant KRAS sequence and the lipid) are linked to one another by a linker, for example, a linker including one or more polyethylene glycol blocks. In one example, the linker is:

##STR00005##

[0050] In a specific example, a mutant KRAS sequence (e.g., any one of SEQ ID NOS:1-7 or 22, or any one of SEQ ID NOS:23-30) is bonded at its N-terminus through a Cys residue to a linker, and the linker is bonded to a lipid, where the linker is:

##STR00006##

[0051] and the lipid is:

##STR00007##

[0052] or a salt thereof.

[0053] The invention also provides compositions that include one or more compounds of the invention, together with a pharmaceutically acceptable carrier or diluent. Optionally, the compositions can include seven different compounds as described above, where each of the seven different compounds includes a different sequence selected from SEQ ID NOS:1-7 and 23 or SEQ ID NOS:23-30. Compositions including subsets (e.g., 2, 3, 4, 5, or 6) of these compounds are also included in the invention. Different compounds included in the compositions of the invention can optionally each include the same or different KRAS sequences, linkers, and/or lipids.

[0054] The compositions of the invention can be used in methods to induce immune responses to the KRAS sequences of the compounds. Accordingly, the compositions can be referred to as immunogenic or vaccine compositions. The compositions can thus optionally include or be administered with one or more adjuvants. In one example, the adjuvant used can include a CpG oligonucleotide (e.g., 5-TCGTCGTTTTGTCGTTTTGTCGTT-3; SEQ ID NO:8; CpG-7909) that, at its 5 end, is bonded or linked by a linker to a lipid, such as the following:

##STR00008##

[0055] or a salt thereof, where X is O or S. Preferably, X is S.

[0056] The CpG oligonucleotide may be directly bonded to the lipid. Alternatively, the linker bonded to the CpG oligonucleotide and to the lipid may be GG. In the CpG oligonucleotide, all internucleoside groups are phosphorothioates (e.g., all internucleoside groups in the compound may be phosphorothioates).

[0057] The compounds and compositions of the invention can be used in therapeutic methods. In particular, the KRAS sequences of the compounds can induce an immune response to KRAS, which is expressed in certain cancer cells. Accordingly, the invention provides methods of treating cancer in a subject (e.g., a human patient) by administering one or more compounds or compositions of the invention to the subject. The invention also includes methods of inducing an immune response against KRAS in a subject (e.g., a human patient) by administering one or more compounds or compositions of the invention to the subject. In various examples, the cancer is selected from the group consisting of pancreatic cancer, lung cancer, and colorectal cancer. Optionally, the methods of the invention can further include administering a compound or composition of the invention in combination with a second (or further) different approach to treatment.

[0058] The invention also provides kits that each contain, for example, a first vessel that includes one or more compounds of the invention, optionally together with a second vessel that includes an adjuvant, such as an adjuvant as described herein.

TABLE-US-00001 TABLE1 (SEQIDNOS:1-7and22) Amph- Peptide Peptide Name Sequence MW(Da) Residues MW(Da) 1 G12D4-21 CYKLVVVGADGVGKSALTI 1893 19 5049 2 G12V4-21 CYKLVVVGAVGVGKSALTI 1877 19 5033 3 G12R4-21 CYKLVVVGARGVGKSALTI 1934 19 5090 4 G12A4-21 CYKLVVVGAAGVGKSALTI 1849 19 5005 5 G1254-21 CYKLVVVGASGVGKSALTI 1865 19 5021 6 G12C4-21 YKLVVVGACGVGKSALTI 1778 19 4934 or or G12T4-21 CYKLVVVGATGVGKSALTI 19 7 G13D4-21 CYKLVVVGAGDVGKSALTI 1790 19 5049

[0059] For each of the Amph-Peptide referred to in Table 1, the lipid poly(ethylene glycol) moiety was conjugated via a maleimide (MAL) -cysteine coupling to the peptide using the following synthon:

[0060] DSPE-amido-dPEG24-amido-dPEG24-MAL

TABLE-US-00002 TABLE2 (SEQIDNOS:23-30) Peptide Name Sequence MW(Da) Residues 1 G12D4-21 YKLVVVGADGVGKSALTI 1790 18 2 G12V4-21 YKLVVVGAVGVGKSALTI 1774 18 3 G12R4-21 YKLVVVGARGVGKSALTI 1831 18 4 G12A4-21 YKLVVVGAAGVGKSALTI 1746 18 5 G1254-21 YKLVVVGASGVGKSALTI 1762 18 6 G12C4-21 YKLVVVGACGVGKSALTI 1778 18 or or G12T4-21 YKLVVVGATGVGKSALTI 18 7 G13D4-21 YKLVVVGAGDVGKSALTI 1790 18

[0061] A compound of the invention may be prepared from a mutant KRAS polypeptide, a linker precursor, and a lipid using methods known in the art. In some variations, a linker precursor is first linked to a lipid, e.g., as follows:

##STR00009##

[0062] Amidation reaction conditions are known in the art, for example, typical amidation conditions include the use of reagents, such as EDC/DMAP, HATU/HOAt, or HBTU/HOAt. Alternatively, the carboxylate may be replaced with an O-succinimide ester, pentrafluorophenyl ester, or tetrafluorophenyl ester. The product of this reaction may then be reacted with a mutant KRAS polypeptide, e.g., as follows:

##STR00010##

[0063] The 1,4-addition reaction may be carried out in an appropriate solvent, e.g., a polar organic solvent or water.

[0064] Alternatively, a compound of the invention may be prepared as follows. A linker precursor may be first linked to a lipid, e.g., as follows:

##STR00011##

[0065] The product may then be reacted with an amine group (e.g., N-terminal amine) in the mutant KRAS polypeptide to produce a compound of the invention:

##STR00012##

[0066] A CpG oligonucleotide may be bonded directly or linked by a linker to the lipid. These compounds may be produced using the ordinary phosphoramidite chemistry known in the art. In some examples, the CpG oligonucleotide or CpG oligonucleotide that, at its 5 end, is bonded to GG may be reacted with the following compound:

##STR00013##

[0067] to produce an intermediate, which upon oxidation with (e.g., phosphite oxidation methods known in the art, e.g., a sulfurizing agent, such as 3-((N,N-dimethylaminomethylidene)amino)-3H-1,2,4-dithiazole-5-thione) and hydrolysis of the cyanoethyl group may produce a compound consisting of CpG oligonucleotide that, at its 5 end, is bonded or linked by a linker to the lipid:

##STR00014##

[0068] or a salt thereof,

[0069] wherein X is O or S.

Dosing

[0070] The dose of each KRAS peptide administered can be in the range of 100 to 5000 g per peptide (e.g., 700 g/peptide, which would yield a peptide dose of 4900 g for a group of 7 peptides).

[0071] If an adjuvant is administered with the KRAS peptide, the dose of the adjuvant can be, on a body weight basis, as high as 0.48 mg/kg. Exemplary dosing regimens are shown below in Table 3.

TABLE-US-00003 TABLE 3 Adjuvant Dosing (e.g. Amph-CpG-7909; FIG. 6) Peptide Dose 1 0.1 mg 700 g for (low) each of 7 peptides Dose 2 0.5 mg 700 g for (medium) each of 7 peptides Dose 3 2.5 mg 700 g for (high) each of 7 peptides

[0072] In order that this invention be more fully understood, the following examples are set forth. These examples are for the purpose of illustration only and are not to be construed as limiting the scope of the invention in any way.

EXAMPLES

Example 1

Amphiphile KRas G12D Elicits an Immune Response

[0073] The efficacy of soluble-KRas (KRas) or amphiphile-KRas (aKRas) (FIG. 6) mutant sequences and an amphiphile-CpG adjuvant (aCpG) to elicit an immune response was determined. In these experiments five-times as much aCpG as amphiphile-KRas was used because CpG escalation experiments suggested that this dose may be more efficacious.

[0074] The experimental design included the following 4 groups of C57BL/6 mice immunized with the listed compounds (n=5 for each group):

[0075] 1. KRas G12D+aCpG

[0076] 2. aKRas G12D+aCpG

[0077] 3. KRas G12D+pIC

[0078] 4. No immunization

[0079] PolyIC (pIC) was used as a benchmark adjuvant control.

[0080] The adjuvant, as well as peptide stock, solutions were dissolved in H.sub.2O. Final injections were diluted with 1 PBS (phosphate buffered saline).

[0081] The aKRas peptides used were 18 mer sequences of mutant sequences with a G12D substitution (amino acids 4-21 of wild-type 4 YKLVVVGAGGVGKSALTI (SEQ ID NO:9)). 20 g in 100 l were used for each injection.

[0082] The aCpG sequence used was the CpG1826 sequence (5-tccatgacgttcctgacgtt-3; SEQ ID NO:10) with two guanines added at the 5 end (5-gg tccatgacgttcctgacgtt-3; SEQ ID NO:11) at a concentration of 5 nmol for each 100 l injection. CpG1826 is an optimal mouse sequence while CpG7909 is optimal for humans and poorly active in mice. CpG1826 and CpG7909 are in the same CpG class (class B) and generally have similar activity profiles in their respective species.

[0083] 50 g of pIC were used for each 100 l injection.

[0084] Primer immunizations were given subcutaneously (s.c.) into the tail base (day 0) with one booster dose after 2 weeks (day 14). ELISpot analysis for IFN on splenocytes was performed, using standard protocols, 8 days after booster dose administration (day 21). Splenocytes (10.sup.6 cells/well) were activated with 5 g/well of G12D sequence 18 mer (aa4-21) .fwdarw.YKLVVVGADGVGKSALTI (SEQ ID NO:1). As shown in FIG. 1, aKRas in combination with aCpG activated splenocytes while soluble KRas in combination with aCpG, and the untreated and soluble KRas and pIC control groups, did not.

Example 2

Amphiphile KRas G12R and G12V Elicit an Immune Response

[0085] The efficacy of additional soluble-KRas (KRas) or amphiphile-KRas (aKRas) mutant sequences and an amphiphile-CpG adjuvant (aCpG) or soluble CpG (CpG) to elicit an immune response was determined.

[0086] The experimental design included the following 7 groups of C57BL/6 mice immunized with the listed compounds (n=5 for each group):

[0087] 1. KRas G12R+CpG

[0088] 2. KRas G12V+CpG

[0089] 3. KRas G12R+pIC

[0090] 4. KRas G12V+pIC

[0091] 5. aKRas G12R+aCpG

[0092] 6. aKRas G12V+aCpG

[0093] 7. No immunization

[0094] The adjuvant as well as peptide stock solutions are dissolved in H.sub.2O. Final injections are diluted with 1 PBS.

[0095] The aKras peptides used were 18 mer sequences of mutant sequences with G12R/V substitutions (SEQ amino acids 4-21 of wild-type .fwdarw.YKLVVVGAGGVGKSALTI (SEQ ID NO:9)). 20 g in 100 l were used for each injection.

[0096] The aCpG sequence used was the CpG1826 sequence (5-tccatgacgttcctgacgtt-3; SEQ ID NO:10) with two guanines added at the 5 end (5-gg tccatgacgttcctgacgtt-3; SEQ ID NO:11) at a concentration of 5 nmol for each 100 l injection.

[0097] 50 g of pIC were used for each 100 linjection.

[0098] Primer immunizations were given subcutaneously (s.c.) into the tail base with one booster dose after 2 weeks (d14).

[0099] ELISpot analysis for IFN on splenocytes was performed, using standard protocols, 7 days after booster dose administration (d21). Splenocytes (10.sup.6 cells/well) were activated with 5 g/well of either: the G12V sequence 18 mer (aa4-21) .fwdarw.YKLVVVGAVGVGKSALTI (SEQ ID NO:2) or the G12R sequence 18 mer (aa4-21) .fwdarw.YKLVVVGARGVGKSALTI (SEQ ID NO:3).

[0100] As shown in FIG. 2, both aKRas G12R and G12V in combination with aCpG activated splenocytes while neither soluble KRasG12R nor G12V in combination with aCpG, and the untreated and soluble KRas and pIC control groups, did not.

Example 3

Amphiphile KRas G12D Elicits a CD8+T-cell Immune Response

[0101] In wild-type B6 mice it is difficult to elicit a Kras-specific CD8+T-cell response. B6 mice transgenic for the human HLA gene, A2.1, were immunized either with the soluble or amphiphile-conjugated forms of the KRas G12D antigen, as well as adjuvant (CpG or aCpG). The A2.1 allele is known to mount responses to the KRas peptides in humans.

[0102] The experimental design included the following 4 groups of B6 HLA-A2.1 Tg mice immunized with the listed compounds (n=5 for each group):

[0103] 1. KRas wild-type+CpG

[0104] 2. KRas 12D+CpG

[0105] 3. aKRas 12D+aCpG

[0106] 4. No immunization

[0107] The adjuvant as well as peptide stock solutions were dissolved in H.sub.2O. Final injections were diluted with 1 PBS.

[0108] The aKRas peptides used were 18 mer sequences of mutant sequences with a G12D substitution (amino acids 4-21 of wild-type .fwdarw.YKLVVVGAGGVGKSALTI (SEQ ID NO:9)). 20 g in 100 l were used for each injection.

[0109] The aCpG sequence used was the CpG1826 sequence (5-tccatgacgttcctgacgtt-3; SEQ ID NO:10) with two guanines added at the 5 end (5-gg tccatgacgttcctgacgtt-3; SEQ ID NO:11) at a concentration of 5 nmol for each 100 l injection.

[0110] The vaccine was administered subcutaneously to female mice bilaterally at the tail base at 50 l per side. Two booster doses were given in two-week intervals.

[0111] Intracellular cytokine staining (ICS) analysis for IFN on peripheral blood mononuclear cells (PBMCs) and ELISpot analysis for IFN on splenocytes was performed, using standard protocols, 7 days after the second and third booster dose, respectively.

[0112] PBMCs/Splenocytes (10.sup.6 cells/well and 210.sup.6 cells/well) were activated with 5 g/well of either SEQ ID NO:1 or one of SEQ ID NOS:12-20 as shown below:

[0113] Specific mutant peptides are given only to the mice that were immunized with the corresponding 18 mer.

[0114] FIG. 3 shows a KRas-specific CD8+T-cell response for mice immunized with the combination of aKRas G12D and aCpG, but not for soluble KRas G12D in combination with CpG or soluble wild-type KRas in combination with CpG.

Example 4

Analysis for Higher Antigen Concentration and Different Dose Intervals

[0115] The concentration of antigen was increased from 20 g to 50 g and bi-weekly (bw) or weekly (w) dose intervals were tested.

[0116] The experimental design included the following 5 groups of C57BL/6 mice immunized with the listed compounds (n=5 for each group):

[0117] 1. KRas 12D+CpG1826 (bw)

[0118] 2. KRas 12D+CpG1826 (w)

[0119] 3. aKRas 12D+aCpG1826 (bw)

[0120] 4. aKRas 12D+aCpG1826 (w)

[0121] 5. No immunization

[0122] The adjuvant as well as peptide stock solutions were dissolved in H.sub.2O. Final injections were diluted with 1 PBS.

[0123] The aKRas peptides used were 18 mer sequences of mutant sequences with a G12D substitution (amino acids 4-21 of wild-type .fwdarw.YKLVVVGAGGVGKSALTI (SEQ ID NO:9)). 50 g in 100 lwere used for each injection.

[0124] The aCpG sequence used was the CpG1826 sequence (5-tccatgacgttcctgacgtt-3; SEQ ID NO:10) with two guanines added at the 5 end (5-gg tccatgacgttcctgacgtt-3; SEQ ID NO:11) at a concentration of 5nmo1 for each 100 linjection.

[0125] Primer immunizations were given subcutaneously (s.c.) into the tail base with one booster dose after 2 weeks.

[0126] ELISpot analysis for IFN, and CBA (cytometric bead array) analysis for IL6, IL10, IL12, TNF, IFN, and MCP1 was performed on splenocytes, using standard protocols, 7 days after booster dose administration. Splenocytes (10.sup.6 cells/well) were activated with 5 g/well of either SEQ ID NO:1 or one of SEQ ID NOS:12-21 as shown below:

[0127] Specific mutant peptides were given only to the mice that were immunized with the corresponding 18 mer.

[0128] Separate plates were prepared for either short (9 mer and lOmer) or long (17 mer and 18 mer) peptide stimulations. Cells from No immunization control mice were stimulated with all stimuli.

[0129] FIG. 4 shows a KRas-specific CD8+T-cell response (9 mers) and CD8+or CD4+T-cell response (18 mer) for mice immunized with the combination of aKRas G12D and aCpG, but not for soluble KRas G12D in combination with CpG or the untreated control.

Example 5

Further Analysis of KRAS Amphiphiles

[0130] As described in the above Examples, the potential immunogenicity of a mutant KRAS peptide-amphiphile vaccine with aCpG adjuvant has been demonstrated in a C57BL/6 mouse model. These studies, and further studies, are summarized in the Table 4 and demonstrate that although mice historically mount poorer immunologic responses than humans to mutant KRAS peptides, due to species differences in MHC binding, [0131] subcutaneous administration of mutated KRAS Amph-Peptides (G12D (FIG. 6), G12R, and G12V) is effective at inducing KRAS-specific splenic T-cells in mice as identified by ELISPOT and cytokine bead array (FIG. 1, FIG. 2, FIG. 4, and FIG. 5) [0132] mutated KRAS Amph-Peptides (G12D, G12R, and G12V) combined with aCpG as the adjuvant are more effective at inducing KRAS-specific splenic T-cells than mutated KRAS peptides combined with either soluble CpG or polyinosinic:polycytidylic acid (poly IC) as the adjuvant (FIG. 1, FIG. 2, and FIG. 5) and [0133] mutated KRAS Amph--Peptides (G12D) combined with aCpG are more effective than soluble KRAS peptides combined with soluble CpG at eliciting both CD4 and CD8 T-cell responses as demonstrated through effector cytokine response upon restimulation with either long (18-mer) or minimal (9-mer) peptide epitopes (FIG. 4).

TABLE-US-00004 TABLE 4 Test Article ROA/Treatment Doses/Dose Volume Regimen Results 4 groups: ROA: SC ELISPOT (IFN) 1) KRAS G12D + CpG; Prime: Day 0 Results: 2) Amph-KRAS G12D + aCpG; Boost: Day 14 Amph-Peptide G12D + aCpG 3) KRAS G12D + Poly IC; produced superior splenic 4) No Treatment T-cell responses when KRAS peptides (amphiphilic or soluble) compared to either were dosed at 20 g; aCpG-1826 at 5 nmoL; soluble G12D + and Poly IC at 50 g; stock soluble CpG or Poly IC solutions were dissolved in water and final dose solutions in phosphate- buffered saline (PBS). Dose volume: 100 L administered divided as a SC injection on either side of the tail head (50 L per injection site) 7 groups: ROA: SC ELISPOT (IFN) 1) KRAS G12R + CpG; Prime: Day 0 Results: 2) KRAS G12V + CpG Boost: Day 14 KRAS peptide 3) KRAS G12R + Poly IC; sequences are more 4) KRAS G12V + Poly IC; immunogenic when 5) Amph-KRAS G12R + aCpG; conjugated to a lipid 6) Amph-KRAS G12V + aCpG; moiety than are 7) No Treatment soluble peptides KRAS peptides (amphiphilic or soluble) were dosed at 20 g; aCpG-1826 at 5 nmoL; and Poly IC at 50 g; stock solutions were dissolved in water and final dose solutions in PBS. Dose volume: 100 L administered divided as a SC injection on either side of the tail head (50 L per injection site) 5 groups: ROA: SC ELISPOT (IFN) and 1) KRAS G12D + CpG (bw); 2 doses (prime + boost) CBA Results: 2) KRAS G12D + CpG (w); were administered for the Amphiphiles elicited 3) Amph-KRAS G12D + aCpG (bw); animals treated endogenous CD8 4) Amph-KRAS G12D + aCpG (w); biweekly (bw) and 3 and CD4 T-cell 5) No treatment doses (prime + 2 boosts) responses against KRAS peptides (amphiphilic or soluble) were administered to the mutant KRAS in were dosed at 50 g; aCpG-1826 at 5 nmol; animals treated weekly C57BL/6 mice as stock solutions were dissolved in (w): assessed by both water and final dose solutions in PBS. Prime: Day 0 (w and bw) ELISPOT (IFN) Dose volume: 100 L administered Boost: Day 7 (w) and/or cytokine bead divided as a SC injection on either side Boost: Day 14 (w and bw) array (IFN, TNF and IL-6) of the tail head (50 L per injection site) CBA = cytometric bead array; ELISPOT = enzyme-linked immunospot; IFN = interferon gamma; IL-6 = interleukin 6; KRAS = Kirsten Rat Sarcoma; poly IC = polyinosinic:polycytidylic acid; ROA = route of administration; SC = subcutaneous; TNF = tumor necrosis factor alpha

Other Embodiments

[0134] While the invention has been described in connection with specific embodiments thereof, it will be understood that it is capable of further modifications and this application is intended to cover any variations, uses, or adaptations of the invention following, in general, the principles of the invention and including such departures from the present disclosure come within known or customary practice within the art to which the invention pertains and may be applied to the essential features hereinbefore set forth.

[0135] All publications, patents, and patent applications are herein incorporated by reference in their entirety to the same extent as if each individual publication, patent or patent application was specifically and individually indicated to be incorporated by reference in its entirety.

[0136] Some embodiments of the invention are within the following numbered paragraphs.

[0137] 1. A compound comprising a mutant KRAS sequence and a lipid, wherein the mutant KRAS sequence is conjugated to the lipid by a linker, and (i) the linker comprises one or more polyethylene glycol blocks, (ii) the lipid is 1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE), and (iii) the mutant KRAS sequence comprises or consists of an amino acid sequence selected from the group consisting of YKLVVVGADGVGKSALTI (SEQ ID NO:23), YKLVVVGAVGVGKSALTI (SEQ ID NO:24), YKLVVVGARGVGKSALTI (SEQ ID NO:25), YKLVVVGAAGVGKSALTI (SEQ ID NO:26), YKLVVVGASGVGKSALTI (SEQ ID NO:27), YKLVVVGACGVGKSALTI (SEQ ID NO:28), YKLVVVGATGVGKSALTI (SEQ ID NO:29), and YKLVVVGAGDVGKSALTI (SEQ ID NO:30).

[0138] 2. A compound comprising a mutant KRAS sequence and a lipid, wherein the mutant KRAS sequence is conjugated to the lipid by a linker, and (i) the linker comprises one or more polyethylene glycol blocks, (ii) the lipid is 1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE), and (iii) the mutant KRAS sequence comprises or consists of an amino acid sequence selected from the group consisting of CYKLVVVGADGVGKSALTI (SEQ ID NO:1), CYKLVVVGAVGVGKSALTI (SEQ ID NO:2), CYKLVVVGARGVGKSALTI (SEQ ID NO:3), CYKLVVVGAAGVGKSALTI (SEQ ID NO:4), CYKLVVVGASGVGKSALTI (SEQ ID NO:5), CYKLVVVGACGVGKSALTI (SEQ ID NO:6), CYKLVVVGATGVGKSALTI (SEQ ID NO:22), and CYKLVVVGAGDVGKSALTI (SEQ ID NO:7).

[0139] 3. The compound of paragraph 1 or 2, wherein the mutant KRAS sequence, at its N-terminus, is conjugated to the linker through a cysteine-maleimide linkage.

[0140] 4. The compound of any one of paragraphs 1-3, wherein linker comprises 48 repeat units of polyethylene glycol.

[0141] 5. The compound of paragraph 1 or 2, wherein the mutant KRAS sequence, at its N-terminus, is conjugated to the following structure:

##STR00015##

[0142] 6. A composition comprising one or more compounds of any one of paragraphs 1-5 and a pharmaceutically acceptable carrier.

[0143] 7. The composition of paragraph 6, wherein the composition comprises (1) a compound comprising the amino acid sequence YKLVVVGADGVGKSALTI (SEQ ID NO:23), (2) a compound comprising the amino acid sequence YKLVVVGAVGVGKSALTI (SEQ ID NO:24), (3) a compound comprising the amino acid sequence YKLVVVGARGVGKSALTI (SEQ ID NO:25), (4) a compound comprising the amino acid sequence YKLVVVGAAGVGKSALTI (SEQ ID NO:26), (5) a compound comprising the amino acid sequence YKLVVVGASGVGKSALTI (SEQ ID NO:27), (6) a compound comprising the amino acid sequence YKLVVVGACGVGKSALTI (SEQ ID NO:28) or a compound comprising the amino acid sequence YKLVVVGATGVGKSALTI (SEQ ID NO:29), and (7) a compound comprising the amino acid sequence YKLVVVGAGDVGKSALTI (SEQ ID NO:30).

[0144] 8. The composition of paragraph 6, wherein the composition comprises (1) a compound comprising the amino acid sequence CYKLVVVGADGVGKSALTI (SEQ ID NO:1), (2) a compound comprising the amino acid sequence CYKLVVVGAVGVGKSALTI (SEQ ID NO:2), (3) a compound comprising the amino acid sequence CYKLVVVGARGVGKSALTI (SEQ ID NO:3), (4) a compound comprising the amino acid sequence CYKLVVVGAAGVGKSALTI (SEQ ID NO:4), (5) a compound comprising the amino acid sequence CYKLVVVGASGVGKSALTI (SEQ ID NO:5), (6) a compound comprising the amino acid sequence CYKLVVVGACGVGKSALTI (SEQ ID NO:6) or a compound comprising the amino acid sequence CYKLVVVGATGVGKSALTI (SEQ ID NO:22), and (7) a compound comprising the amino acid sequence CYKLVVVGAGDVGKSALTI (SEQ ID NO:7).

[0145] 9. The composition of any one of paragraphs 6-8, wherein the composition further comprises a compound consisting of the nucleotide sequence 5-TCGTCGTTTTGTCGTTTTGTCGTT-3 (SEQ ID NO:8), which, at its 5 end, is bonded or linked by a linker to the following lipid:

##STR00016##

[0146] or a salt thereof,

[0147] wherein X is O or S.

[0148] 10. The composition of paragraph 6, wherein the composition comprises 700 g of each compound.

[0149] 11. A method of treating a cancer in a human patient, the method comprising administering the composition of any one of paragraphs 6-10 to the patient.

[0150] 12. The method of paragraph 11, wherein the method further comprises administering an adjuvant.

[0151] 13. The method of paragraph 12, wherein the adjuvant comprises a CpG nucleotide sequence.

[0152] 14. The method of paragraph 13, wherein the CpG nucleotide sequence comprises 5-TCGTCGTTTTGTCGTTTTGTCGTT-3 (SEQ ID NO: 8).

[0153] 15. The method of any one of paragraphs 12-14, wherein 0.1 mg, 0.5 mg, or 2.5 mg of the adjuvant is administered.

[0154] 16. The method of paragraph 10, wherein the method further comprises administering to the patient a compound consisting of the nucleotide sequence 5-TCGTCGTTTTGTCGTTTTGTCGTT-3 (SEQ ID NO:8), which, at its 5 end, is bonded or linked by a linker to the following lipid:

##STR00017##

[0155] or a salt thereof,

[0156] wherein X is O or S.

[0157] 17. The composition of paragraph 9 or the method of paragraph 16, wherein the nucleotide sequence is bonded to the lipid.

[0158] 18. The method of paragraph 16 or 17, wherein 0.1 mg, 0.5 mg, or 2.5 mg of the compound consisting of the nucleotide sequence 5-TCGTCGTTTTGTCGTTTTGTCGTT-3 (SEQ ID NO:8), which, at its 5 end, is bonded or linked by a linker to the following lipid:

##STR00018##

[0159] or a salt thereof,

[0160] wherein X is O or S,

[0161] is administered.

[0162] 19. The method of any one of paragraphs 11-16 or 18, wherein the cancer is a pancreatic cancer, a lung cancer, or a colorectal cancer.

[0163] 20. The method of any one of paragraphs 11-16 or 18, wherein all internucleoside groups connecting the nucleosides in 5-TCGTCGTTTTGTCGTTTTGTCGTT-3 (SEQ ID NO:8) are phosphorothioates.

[0164] 21. A kit comprising (i) a compound of any one of paragraphs 1-5, or a composition of any one of paragraphs 6-8, and (ii) a compound consisting of the nucleotide sequence 5-TCGTCGTTTTGTCGTTTTGTCGTT-3 (SEQ ID NO:8), which, at its 5 end, is bonded or linked by a linker to the following lipid:

##STR00019##

[0165] or a salt thereof,

[0166] wherein X is O or S.

[0167] Other embodiments are within the following claims.