Lecithin compositions and methods of making and using such lecithin compositions

Abstract

The present invention is directed towards compositions comprising a lecithin; a sugar alcohol, a sugar, or combinations of any thereof; and an alcohol. Compositions comprising a water soluble surfactant are further disclosed. Methods of separating oil from a mixture are disclosed. Methods of dispersing an oil are further disclosed. Compositions comprising a water-dilutable microemulsion are disclosed. Methods of making chewing gum are disclosed. Methods of recovering oil from a mixture are further disclosed.

Claims

1. A microemulsion comprising: a lecithin; a sugar alcohol; a cosurfactant selected from the group consisting of polysorbates, sorbitan esters, polyglyceril esters, sucrose esters, alkyl polyglucosides, alkyl polyglycosides, poloxamers, an ethylene oxide/propylene oxide block copolymer, ethoxylated fatty acids, ethoxylated monoglycerides, sodium dodecyl sulfate (SDS), sodium lauryl sulfate (SLS), quillaja, rhamnolipids, sophorolipids, and combinations of any thereof; a water soluble alcohol; a plant oil; and water.

2. The microemulsion of claim 1, wherein the lecithin is selected from the group consisting of crude lecithin, fluid lecithin, deoiled lecithin, fractionated lecithin, high phosphatidyl choline (PC) fraction lecithin, acetylated lecithin, hydrolyzed lecithin, hydroxylated lecithin, lecithin comprising enzyme modified phospholipids, and combinations of any thereof.

3. The microemulsion of claim 1, wherein the sugar alcohol comprises 3-12 carbon atoms.

4. The microemulsion of claim 3, wherein the sugar alcohol comprises a solution having from about 55% of the sugar alcohol to about 85% of the sugar alcohol.

5. The microemulsion of claim 1, wherein the sugar alcohol is present in an amount of at least 5% by weight of the microemulsion.

6. The microemulsion of claim 1, wherein the water soluble alcohol is selected from the group consisting of methanol, ethanol, propanol, butanol, and combinations of any thereof.

7. The microemulsion of claim 1, wherein the water soluble alcohol is present in an amount of less than 10% by weight of the microemulsion.

8. The microemulsion of 1, further comprising a sugar.

9. The microemulsion of claim 8, wherein the sugar is selected from the group consisting of corn syrup, high fructose corn syrup, glucose, fructose, sucrose, and combinations of any thereof.

10. The microemulsion of claim 6, wherein the water soluble alcohol is the ethanol.

11. A water soluble surfactant blend in the form of a microemulsion comprising: a cosurfactant selected from the group consisting of polysorbates, sorbitan esters, polyglyceril esters, sucrose esters, alkyl polyglucosides, alkyl polyglycosides, poloxamers, an ethylene oxide/propylene oxide block copolymer, ethoxylated fatty acids, ethoxylated monoglycerides, sodium dodecyl sulfate (SDS), sodium lauryl sulfate (SLS), quillaja, rhamnolipids, sophorolipids, and combinations of any thereof; about 5-60% of a lecithin; about 5-60% of a sugar alcohol; less than 15% of a water soluble alcohol; a plant oil; and water.

12. The water-soluble surfactant blend of claim 11, wherein the lecithin is the deoiled lecithin or the fluid lecithin.

13. The water-soluble surfactant blend of claim 11, wherein the sugar alcohol is selected from the group consisting of sorbitol, erythritol, xylitol, mannitol, lactitol, malitol, isomalt, and combinations of any thereof.

14. The water-soluble surfactant blend of claim 11, wherein a ratio of the sugar alcohol to the alcohol is from about 60:40 to about 90:10.

15. The water-soluble surfactant blend of claim 11, wherein the water soluble alcohol is ethanol.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) FIG. 1 shows a picture of embodiments of compositions of the present invention.

(2) FIG. 2 shows a picture of additional embodiments of a composition of the present invention.

(3) FIG. 3a shows a picture of additional embodiments of compositions of the present invention.

(4) FIG. 3b shows a picture of additional embodiments of compositions of the present invention.

(5) FIG. 4 shows a picture of additional embodiments of compositions of the present invention.

(6) FIG. 5a shows a picture of additional embodiments of compositions of the present invention.

(7) FIG. 5b shows a picture of additional embodiments of compositions of the present invention.

(8) FIG. 6 shows a picture of embodiments of compositions resulting from methods of the present invention.

(9) FIG. 7 shows a picture of embodiments of compositions resulting from methods of the present invention.

(10) FIG. 8 shows a picture of embodiments of compositions resulting from methods of the present invention.

(11) FIG. 9 shows a picture of embodiments of compositions resulting from methods of the present invention.

(12) FIG. 10 shows a picture of embodiments of compositions resulting from methods of the present invention.

DETAILED DESCRIPTION OF THE INVENTION

(13) In one embodiment, the present invention is directed towards compositions comprising a lecithin; a surfactant; and an oil. In further embodiments, the compositions comprise silica. In yet further embodiments, the compositions comprise a sugar alcohol, a sugar, or combinations of any thereof. In still further embodiments, the compositions comprise an alcohol.

(14) In another embodiment, the present invention is directed towards compositions comprising a lecithin; a sugar alcohol, a sugar, or combinations of any thereof; and an alcohol.

(15) In another embodiment, the present invention is directed towards water soluble surfactant blends comprising about 0-60% of a cosurfactant; about 5-60% of a lecithin; about 5-60% of a sugar alcohol, a sugar, or combinations of any thereof; and less than about 15% of an alcohol.

(16) In another embodiment, the present invention is directed towards methods of recovering oil from a mixture, comprising adding a composition to the mixture; wherein the composition comprises a lecithin cosurfactant blend comprising lecithin and a cosurfactant, the cosurfactant having a hydrophilic-lipophilic balance value of between 12 and 16 and the blend having a hydrophilic-lipophilic balance value of between 10 and 18.

(17) In another embodiment, the present invention is directed towards methods of separating oil from a mixture comprising adding the composition or the water soluble surfactant blend of the embodiments of this invention to the mixture.

(18) In another embodiment, the present invention is directed towards methods of dispersing an oil comprising adding the composition or the water soluble surfactant blend of the previous embodiments to the oil.

(19) In another embodiment, the present invention is directed towards water-dilutable microemulsions comprising the composition or the water soluble surfactant blend of the previous embodiments and a compound selected from the group consisting of a soy methyl ester, limonene, an oil, and combinations of any thereof.

(20) In another embodiment, the present invention is directed towards methods of making chewing gum comprising combining the composition or the water soluble surfactant blend of the previous embodiments with a flavoring, thus forming a flavoring blend, and adding the flavoring blend to a gum base.

(21) In another embodiment, the present invention is directed towards methods of recovering oil from a mixture, comprising adding a composition to the mixture, wherein the composition comprises a lecithin cosurfactant blend comprising 50-90% lecithin and a cosurfactant, the cosurfactant having a hydrophilic-lipophilic balance value of between 12 and 16 and the blend having a hydrophilic-lipophilic balance value of between 10 and 18.

(22) In another embodiment, the present invention is directed towards compositions comprising a surfactant and a compound selected from the group consisting of lecithin, an oil, silica, and combinations of any thereof.

(23) In a further embodiment, the sugar alcohol or the sugar is a solution. In still further embodiments, the compositions of the present invention are liquid.

(24) Microemulsions are clear, isotropic, thermodynamically stable liquid mixtures including oil, water, and a surfactant (cosurfactant). The water phase may contain salt(s) and/or other ingredients. Microemulsions may be prepared from a large number of components. In contrast to ordinary emulsions, microemulsions form upon simple mixing of the components and do not require high shear conditions. In ternary systems, such as microemulsions, where two immiscible phases (water and oil) are present next to the surfactant phase, the surfactant molecules form a monolayer at the interface between oil and water, with the hydrophobic tails of the surfactant molecules dissolved in the oil phase and the hydrophilic head groups in the aqueous phase. Comparable to the binary systems (water/surfactant or oil/surfactant), self-assembled structures of different morphologies can be obtained ranging from (inverted) spherical and cylindrical micelles to lamellar phases and bi-continuous microemulsions. A water-in-oil microemulsion is an optically transparent mixture including oil, water, and surfactant. Water droplets are in a continuous oil phase stabilized by surfactant.

(25) The present invention contemplates many types of surfactant and cosurfactants. The surfactant or cosurfactant may comprise one or more cationic surfactants, one or more anionic surfactants, one or more nonionic surfactants, biobased emulsifiers, or combinations of any thereof. In various embodiments, the surfactant or cosurfactant or combinations may have a hydrophilic-lipophilic balance ranging from 10.0 to 18.0.

(26) Cationic surfactants suitable for use in the disclosed compositions and methods include, but are not limited to, fatty amine salts; fatty alkyl quaternary amines including primary, secondary, and tertiary amines; ester amines and the corresponding ethoxylated ester amines; and combinations of any thereof.

(27) Anionic surfactants suitable for use in the disclosed compositions and methods include, but are not limited to, sodium and potassium salts of straight-chain fatty acids, polyoxyethylenated fatty alcohol carboxylates, linear alkyl benzene sulfonates, alpha olefin sulfonates, sulfonated fatty acid methyl ester, aryl alkanesulfonates, sulfosuccinate esters, alkyldiphenylether(di)sulfonates, alkylnaphthalenesulfonates, isoethionates, alkylether sulfates, sulfonated oils, fatty acid monoethanolamide sulfates, polyoxyethylene fatty acid monoethanolamide sulfates, aliphatic phosphate esters, nonylphenolphosphate esters, sarcosinates, fluorinated anionics, anionic surfactants derived from oleochemicals, and combinations of any thereof.

(28) Nonionic surfactants suitable for use in the disclosed compositions and methods include, but are not limited to, sorbitan monostearate, polyoxyethylene ester of rosin, polyoxyethylene dodecyl mono ether, polyoxyethylene-polyoxypropylene block copolymer, polyoxyethylene monolaurate, polyoxyethylene monohexadecyl ether, polyoxyethylene monooleate, polyoxyethylene mono(cis-9-octadecenyl)ether, polyoxyethylene monostearate, polyoxyethylene monooctadecyl ether, polyoxyethylene dioleate, polyoxyethylene distearate, polyoxyethylene sorbitan monolaurate polyoxyethylene sorbitan monooleate, polyoxyethylene sorbitan monopalmitate, polyoxyethylene sorbitan monostearate, polyoxyethylene sorbitan trioleate, polyoxyethylene sorbitan tristearate, polyglycerol ester of oleic acid, polyoxyethylene sorbitol hexastearate, polyoxyethylene monotetradecyl ether, polyoxyethylene sorbitol hexaoleate, fatty acids, tall-oil, sorbitol hexaesters, ethoxylated castor oil, ethoxylated soybean oil, rapeseed oil ethoxylate, ethoxylated fatty acids, ethoxylated fatty alcohols, ethoxylated polyoxyethylene sorbitol tetraoleate, glycerol and polyethylene glycol mixed esters, alcohols, polyglycerol esters, monoglycerides, sucrose esters, alkyl polyglycosides, polysorbates, fatty alkanolamides, polyglycol ethers, derivatives of any thereof, and combinations of any thereof.

(29) In still further embodiments, the surfactant or cosurfactant is selected from the group consisting of polysorbates, sorbitan esters, polyglyceril esters, sucrose esters, alkyl polyglucosides, alkyl polyglycosides, polaxmers, PLURONIC brand ethylene oxide/propylene oxide block copolymer, ethoxylated fatty acids, ethoxylated monoglycerides, propylene glycol, polyethylene glycol esters, sodium dodecyl sulfate (SDS), sodium lauryl sulfate (SLS), quillaja, rhamnolipids, sophorolipids, derivatives of any thereof, and combinations of any thereof. In preferred embodiments, the surfactant or cosurfactant is the alkyl polyglycosides.

(30) In further embodiments, the surfactant or cosurfactant is present in an amount of about 0-60% by weight of the composition. In preferred embodiments, the surfactant or cosurfactant is present in an amount about 40-60% by weight of the composition. In more preferred embodiments, the surfactant or cosurfactant is present in an amount of about 50% by weight of the composition.

(31) Lecithin is a versatile emulsifier. Commercial lecithin has a hydrophilic-lipophilic balance (HLB) in a range of 4-10, depending on whether the lecithin is a fluid containing oil or is deoiled. There are many modified lecithins, including fractionated lecithin, lecithin with enzyme modified phospholipids, acetylated lecithin, hydrolyzed lecithin, and hydroxylated lecithin. Lecithin in its various forms has dispersion properties but is not completely water soluble. Table 1, below, shows the dispersion rating and emulsification capacity of various types of lecithin. In Table 1, a rating of 1=poor and a rating of 5=excellent.

(32) TABLE-US-00001 TABLE 1 Dispersion and Emulsifying Capacity of Soy Lecithin Dispersion Emulsification Type of Lecithin Rating Capacity Standard fluid lecithin 1 2 Standard deoiled - powder 0 2 High phosphatidyl choline fraction 0 4 Acetylated fluid/oil free 4 4 Hydrolyzed fluid/oil free 4 5 Hydroxylated fluid/oil free 5 5

(33) Lecithin is comprised of four main phospholipids: phosphatidyl choline (PC), phosphatidyl ethanolamine (PE), phosphatidyl inositol (PI), and phosphatidic acid (PA). Each of these phospholipids has unique functionality, and some applications desire one specific phospholipid for its properties. In such cases, fractionation is required. Alcohol fractionation of lecithin is known. This fractionation is based on the differences in solubility of the phospholipids in alcohol. Fluid and deoiled lecithin have different soluble fractions in 100% ethanol. In Table 2, below, the alcohol fractionation of both crude soy lecithin and deoiled soy lecithin are shown.

(34) TABLE-US-00002 TABLE 2 Alcohol Fractionation of Soy Lecithin Phosphatidyl Triglycerides Choline (PC) Yield (%) (%) (%) Crude Lecithin 39 15 Soluble Fraction: Ethanol (100%) 60 22 26 Ethanol (95%) 48 33 15 Ethanol (90%) 23 46 12 Insoluble Fraction: Ethanol (95%) 38 7 85 Deoiled Lecithin 2.5 24 Soluble Fraction: Ethanol (100%) 2.5 52 28 Insoluble Fraction: Ethanol (100%) 2.5 12 72

(35) The present invention contemplates using many types of lecithin, including crude lecithin, fluid lecithin, deoiled lecithin, fractionated lecithin, high phosphatidyl choline (PC) fraction lecithin, acetylated lecithin, hydrolyzed lecithin, hydroxylated lecithin, lecithin comprising enzyme modified phospholipids, and combinations of any thereof. In preferred embodiments, the lecithin is deoiled lecithin or fluid lecithin.

(36) In further embodiments, the lecithin is present in an amount from about 5% to about 60% by weight of the composition. In preferred embodiments, the lecithin is present in an amount from about 5% to about 25% by weight of the composition. In certain preferred embodiments, the lecithin is present in an amount of about 40% by weight of the composition.

(37) The present invention contemplates many types of sugar alcohols, including sugar alcohols comprising 3-12 carbon atoms. In further embodiments, the sugar alcohol is selected from the group consisting of sorbitol, erythritol, xylitol, mannitol, lactitol, maltitol, isomalt, and combinations of any thereof. In preferred embodiments, the sugar alcohol is sorbitol.

(38) In further embodiments, the sugar alcohol comprises a solution having from about 55% of the sugar alcohol to about 85% of the sugar alcohol.

(39) In further embodiments, the sugar alcohol is present in an amount of at least about 5% by weight of the water soluble composition. In yet further embodiments, the sugar alcohol to the alcohol ratio is from about 60:40 to about 90:10.

(40) The present invention contemplates many types of sugars, including corn syrup, high fructose corn syrup, glucose, fructose, sucrose, and combinations of any thereof.

(41) The present invention contemplates many types of alcohols, selected from the group consisting of C1-C4 alcohols including methanol, ethanol, propanol, and butanol; water soluble alcohols; and combinations of any thereof. In preferred embodiments, the alcohol is ethanol. In further embodiments, the alcohol is present in an amount of less than about 10% by weight of the composition.

(42) In further embodiments, the composition comprises silica. In other embodiments, the silica is hydrophilic.

(43) In further embodiments, the composition is water soluble.

(44) In further embodiments, the composition is used to recover: corn oil in a corn to ethanol process, sorghum oil in a sorghum to ethanol process, or combinations thereof.

(45) In further embodiments, the composition is used as a base concentrate to make a microemulsion. In yet further embodiments, the composition is used to form a microemulsion with oil, a flavor, a color, an antioxidant, a bioactive, and combinations of any thereof.

(46) In further embodiments, the composition is used to disperse an oil. In still further embodiments, the oil is selected from the group consisting of mint oil, vegetable oils, essential oils, and combinations of any thereof. In preferred embodiments, the oil is mint oil.

(47) In further embodiments, the composition comprising a surfactant and a compound selected from the group consisting of lecithin, an oil, silica, and combinations of any thereof, comprises an oil selected from the group consisting of canola oil, plant oils, vegetable oils, essential oils, and combinations of any thereof. In preferred embodiments, the oil is canola oil.

(48) In further embodiments, the water soluble surfactant blends of the present invention are combined with a compound selected from the group consisting of soy methyl esters, limonene, mint oil, and combinations of any thereof.

(49) In further embodiments, the methods of separating oil from a mixture contemplate the mixture selected from the group consisting of whole stillage, thin stillage, syrup, feed, water, and combinations of any thereof. In still further embodiments, the mixture comprises the oil.

(50) In further embodiments, the gum base of the present invention comprises an emulsifier.

(51) The present invention contemplates many co-solvents, including short chain fatty alcohols; acids; esters; sugar alcohols including sorbitol, glycerol, ethylene glycol, propylene glycol; triglycerides; diglycerides; food grade mineral oils; plant oils; and combinations of any thereof.

(52) In preferred embodiments, the present invention contemplates methods of recovering oil from a mixture, comprising adding a composition to the mixture, wherein the composition comprises 80% by weight lecithin, 10% by weight ethoxylated monoglycerides, 2% by weight propylene glycol, and 8% by weight soybean oil. In further embodiments, the methods of recovering oil from a mixture further comprises centrifuging the mixture.

(53) The invention is further explained by the following non-limiting examples.

Example 1: Solubility of Lecithin

(54) When ethanol is slowly diluted in water, with the addition of lecithin, the nature of lecithin fractionation changes. In this example, deoiled lecithin was added to a water:ethanol mixture (70:30 ratio), and a very viscous dispersion was obtained. Water and ethanol have the ability to form azeotropes, and the azeotropic mixtures have properties different from water or ethanol. When the water in the mixture was replaced with a 70% sorbitol solution, the deoiled lecithin formed a completely clear, water soluble solution. Therefore, the fractionation of lecithin in alcohol can be reversed by the addition of a sorbitol solution.

Example 2: Fractionation of Lecithin

(55) Based on the dielectric constant values for ethanol (=25), water (=80), and a 70% sorbitol solution (=62), as well as the results of Example 1, at a theoretical dielectric constant of =52, a complete dissolution of lecithin leading to a clear, water soluble solution should be achieved. Common polar solvents were tested to determine if they would indeed give rise to a water soluble lecithin. Surprisingly, DMSO, which has the closest dielectric constant to the theoretical dielectric constant of =52, resulted in totally insoluble lecithin and fractionation. The solvents tested are summarized along with their dielectric constants in Table 3, below. Therefore, dielectric constant alone is not determinative of the ability of a solvent to produce water soluble lecithin.

(56) TABLE-US-00003 TABLE 3 Solvents Which Failed to Produce Water Soluble Lecithin Solvent Dielectric Constant () Propylene Glycol 32 Glycerol 46 DMSO 50 Corn Syrup 56

Example 3: Solubility of Sorbitol in Ethanol

(57) Sugar alcohols can become heavily supersaturated upon cooling. When water is slowly replaced with ethanol, there can be a significant change in the maximum concentration at which the solute is completely soluble. In this example, blends of a 70% sorbitol solution and ethanol were made. The sorbitol/ethanol blends were found to be completely clear at a ratio of 70:30. Blends with greater than 30% ethanol were found to have crystallization of the sorbitol.

Example 4: Solubility of Lecithin in Mixtures

(58) In this example, blends of a sugar alcohol or a sugar were made with ethanol, and 5% by weight deoiled lecithin was added. Deoiled lecithin (5% by weight) was dissolved in a 75:25 ratio of sorbitol/ethanol (made using a 70% sorbitol solution), which resulted in a clear solution. (FIG. 1, vial labeled Sorbitol). The 70% sorbitol solution was replaced with a 70% sucrose solution, and 5% by weight deoiled lecithin was added to a ratio of 75:25 sucrose/ethanol (made using the 70% sucrose solution), which resulted in a clear solution. (FIG. 1, vial labeled Sucrose). The 70% sorbitol solution was replaced with a 70% high fructose corn syrup solution (HFCS, from ADM), and 5% by weight deoiled lecithin was added to a ratio of 75:25 HFCS/ethanol (made using the 70% HFCS solution), which resulted in a clear solution. (FIG. 1, vial labeled HFCS).

Example 5: Comparison of Water Solubility of Lecithin in Mixtures

(59) In this example, 5% by weight a blend of fluid lecithin with a high HLB was added to a 70:30 water/ethanol mixture, and the resulting mixture was compared to a mixture wherein 5% by weight a blend of fluid lecithin with a high HLB was added to a 70:30 sorbitol/ethanol mixture (made using a 70% sorbitol solution). (FIG. 2). Fluid lecithin was found to be more soluble (slightly cloudy appearance) in the 70:30 sorbitol/ethanol mixture (made using a 70% sorbitol solution) as compared to the fluid lecithin in the 70:30 water/ethanol mixture (opaque appearance). (FIG. 2). The presence of sorbitol in a lecithin mixture/dispersion lowers the viscosity of the mixture, which could be exploited in making concentrated dispersions of fluid lecithin which typically have a very high viscosity.

Example 6: Comparison of Water Solubility of Lecithin in Mixtures

(60) In this example, 25% by weight deoiled lecithin (Yelkinol AC, from ADM) was added to a 75:25 water/ethanol mixture (FIG. 3a), and the resulting mixture was compared to a solution wherein 25% by weight deoiled lecithin was added to a 75:25 sorbitol/ethanol solution (made using a 70% sorbitol solution). (FIG. 3b). On the left of FIG. 3a, an opaque water/ethanol (75:25) mixture with the addition of 25% by weight deoiled lecithin is shown. On the right of FIG. 3a, an opaque dispersion made with the water/ethanol (75:25) mixture with the addition of 25% by weight deoiled lecithin is shown. On the left of FIG. 3b, a clear sorbitol/ethanol (75:25) solution made using a 70% sorbitol solution, with the addition of 25% by weight deoiled lecithin is shown. On the right of FIG. 3b, a dispersion made with the sorbitol/ethanol (75:25) solution (made using a 70% sorbitol solution), with the addition of 25% by weight deoiled lecithin is shown. Addition of the sugar alcohol solution reversed the fractionation of lecithin to produce a clear, water soluble product. Further, comparisons of the dispersions shown in FIG. 3a and FIG. 3b show that the presence of sorbitol results in relatively clear dispersions.

Example 7: Water Solubility of Lecithin in Mixtures with Addition of Alkyl Polyglycosides

(61) The effects of the addition of high HLB surfactants to deoiled lecithin in a sorbitol/ethanol solution were studied. Alkyl polyglycosides (APG) based on natural fatty alcohol C8-C14 (Glucopon 425, from BASF) was used as a cosurfactant. A composition was made comprising 50% by weight APG (Glucopon 425), 21% by weight deoiled lecithin (Yelkinol AC), 21% by weight sorbitol (70% sorbitol solution), and 9% by weight ethanol. The resulting composition was a completely clear, flowable liquid with high water solubility. (FIG. 4). On the left of FIG. 4, a clear composition is shown comprising 50% by weight APG, 21% by weight deoiled lecithin, and 30% sorbitol/ethanol solution (70:30) made using a 70% sorbitol solution. On the right of FIG. 4, a relatively clear dispersion made using the composition pictured on the left is shown. The addition of the sugar alcohol solution reversed the fractionation of the lecithin to produce a clear, water soluble product.

Example 8: Water Solubility of Lecithin in Mixtures with Smaller Addition of Alkyl Polyglycosides

(62) A composition was made comprising 40% by weight deoiled lecithin (Yelkinol AC, from ADM), 36% by weight sorbitol (70% sorbitol solution), 18% by weight ethanol, and 6% by weight APG (Glucopon 425, from BASF). The product was completely clear. (FIG. 5a). On the left of FIG. 5a, the clear composition of this example is shown. On the right of FIG. 5a, a dispersion made using the composition of this example is shown. Incorporating a higher concentration of deoiled lecithin is a challenge, as a better dispersed product has better functionality. The composition of this example is fluid, which allows an ease of dispersion and maximizes its functionality.

Example 9: Solubility of Higher Concentration of Lecithin in Mixtures

(63) When a higher concentration of lecithin is used in sorbitol/ethanol-containing compositions, the resulting compositions are very viscous and can be readily diluted in propylene glycol (PG), with product clarity maintained. The composition from Example 8 comprising 40% by weight deoiled lecithin (Yelkinol AC, from ADM), 36% by weight sorbitol (70% sorbitol solution), 18% by weight ethanol, and 6% by weight APG (Glucopon 425, from BASF), was easily soluble in PG. Therefore, PG can be used as a diluent to make more concentrated lecithin products comprising as high as 50% deoiled lecithin, when a sugar alcohol and an alcohol are present in the composition. However, PG alone does not solubilize deoiled lecithin. Generally, the sorbitol/ethanol mixture has been found to improve the solubility of insoluble carbohydrates.

Example 10: Solubility of Lecithin in Mixtures with Addition of Alkyl Polyglycosides

(64) In this example, the addition of alkyl polyglycosides to the clear lecithin in sorbitol/ethanol solution (made using a 70% sorbitol solution) was tested. A blend including 50% alkyl polyglycosides (Glucopon 425), 21% deoiled lecithin, 21% sorbitol (70% solution), and 9% ethanol was found to be easily water soluble. This blend was used as a surfactant base concentrate to make microemulsions with soy methyl ester, limonene, their blends, and mint oil. This blend could have an application in chewing gum. Specifically, the desire for a long-lasting mint flavor has been a challenge to the chewing gum industry. Deoiled lecithin is currently used in gum bases. Use of the water soluble lecithin of the present invention could be advantageous, as addition of the water soluble lecithin of the present invention to sorbitol present in the flavoring portion of the chewing gum will act synergistically with the emulsifier already present in the gum base.

Example 11: Solubility of Lecithin in Mixtures with Addition of Sucrose Esters

(65) In addition to alkyl polyglycosides (APG), there are other high HLB surfactants, including food-grade sucrose esters, which can be added to a composition comprising deoiled lecithin and a sorbitol/ethanol solution. In this example, the surfactant used was a 50% active solution of sucrose esters of fatty acids (stearate/palmitate) (HLB=11) (Sisterna SP 50, from Sisterna). A composition comprising 50% by weight sucrose esters (Sisterna SP 50), 21% by weight deoiled lecithin (Yelkinol AC, from ADM), 21% by weight sorbitol (70% sorbitol solution), and 9% by weight ethanol was made. The resulting composition was a completely clear, low viscosity liquid with high water solubility. The addition of the sugar alcohol solution was found to reverse the fractionation of the lecithin to produce a clear, water soluble product. Therefore, sucrose esters can be used in a surfactant concentrate to make microemulsions, notably without polysorbates.

Example 12: Oil Recovery Using Water Soluble Lecithin Mixture

(66) The blend from Example 7 (50% alkyl polyglycosides (Glucopon 425, from BASF), 21% deoiled lecithin, 21% sorbitol (70% solution), and 9% ethanol) was tested in syrup from a corn dry mill process for oil recovery. Typically, polysorbates (i.e. Polysorbate 80) are used commercially for oil recovery, and therefore Polysorbate 80 was compared to the blend from Example 7. The blend from Example 7 was superior even to the neat alkyl polyglycosides (APG) (Glucopon 425, from BASF). (FIG. 6).

(67) FIG. 6 shows four samples of thin stillage from an ethanol dry grind process, each with either no additive or a different type of corn oil recovery additive. The additive concentration was 200 ppm based on a total weight of 40 g of syrup for the three samples containing additive (either Glucopon 425, Polysorbate 80, or the composition of Example 7). The samples were well mixed and placed in a water bath at 85 C. for 20 minutes. The samples were removed from the bath and centrifuged at 4500 rpm for 4 minutes. The oil recovery from the samples containing additive were compared to the control (no additive).

(68) The vial on the far left of FIG. 6 has no additive and has a small oil layer on the top. The vial second from the left has the additive alkyl polyglycosides (Glucopon 425, from BASF) and has a greater amount of corn oil separated as a top layer compared to the sample with no additive. The vial third from the left has the additive Polysorbate 80 (polyoxyethylene (20) sorbitan monooleate) and has a similar amount of corn oil separated as a top layer compared to the sample with Glucopon 425. The vial on the far right has the additive from Example 7 (50% alkyl polyglycosides (Glucopon 425), 21% deoiled lecithin, 21% sorbitol (70% solution), and 9% ethanol) and has a greater amount of corn oil separated as a top layer compared to either the sample with Glucopon 425 or the sample with Polysorbate 80. Therefore, use of the blend from Example 7 provided the advantages of being 100% bio-based with no petroleum-based derivatives as well as greater yield of corn oil separated from the ethanol dry grind process.

Example 13: Oil Recovery Using Lecithin Mixture Comprising Ethoxylated Monoglycerides and PG

(69) In addition to Example 12, there are other high HLB surfactants that can be added to lecithin to produce a composition useful as an additive in the oil recovery process. A lecithin-cosurfactant blend was prepared by mixing: 80% by weight lecithin (from ADM), 10% by weight ethoxylated monoglycerides (from BASF), 2% by weight propylene glycol, and 8% by weight soybean oil. The blend was mixed at 50 C. under constant stirring for between 30 minutes and 60 minutes, thus producing an amber, transparent lecithin-cosurfactant blend, which can be used as an emulsifier.

(70) This emulsifier blend was used as an additive in corn oil recovery from an ethanol dry mill process. The emulsifier blend of Example 13 was compared to an additive of ethoxylated mono- and di-glycerides, which were both compared to a control (no additive). (FIG. 7). The additive concentration was 200 ppm based on a total weight of 40 g of syrup for the two samples containing additive (either ethoxylated mono- and di-glycerides or the composition of Example 13). The samples were well mixed and placed in a water bath at 85 C. for 20 minutes. The samples were removed from the bath and centrifuged at 4500 rpm for 4 minutes. The oil recovery from the samples containing additive were compared to the control (no additive).

(71) The vial on the left of FIG. 7 has no additive and has a small oil layer on the top. The vial in the middle has the additive ethoxylated mono- and di-glycerides and also has a small amount of corn oil separated as a top layer. The vial on the right has the additive from Example 13 (80% by weight lecithin (from ADM), 10% by weight ethoxylated monoglycerides (from BASF), 2% by weight propylene glycol, and 8% by weight soybean oil) and has a greater amount of corn oil separated as a top layer compared to the control and the sample with the ethoxylated mono- and di-glycerides. Therefore, use of the blend from Example 13 provided the advantage of greater yield of corn oil separated from the ethanol dry grind process.

Example 14: Solubility of Lecithin in Mixtures with Addition of Other Emulsifiers

(72) Instead of using alkyl polyglycosides in combination with a sorbitol/ethanol (75:25) solution made using a 70% sorbitol solution, the addition of 25% by weight deoiled lecithin combined with quillaj a (quillaj a) was used. Quillaja is a natural, generally recognized as safe (GRAS), food-grade surfactant rich in saponins. Quillaja is commonly used as an emulsifier in stabilizing emulsions in food and personal care industries, especially when clear systems are desired. Quillaja can be safely consumed, for example when used in beverage emulsions. In this example, a blend comprising 27% deoiled lecithin, 28% sorbitol (70% solution), and 12% ethanol was pre-made, and 33% Ultra Liquid (Quillaja extract, Desert King, CA) was added to make a clear solution. This solution blend can be used in making microemulsions to solubilize active ingredients, for example 1% weight/volume (w/v) beta carotene (beta carotene in soybean oil). The finished microemulsions can be delivered in a clear, water soluble beverage system. An advantage of the compositions of this example is that the use of any polysorbate has been eliminated.

Example 15: De-Emulsification Functionality of Emulsifiers and Emulsifier Blends

(73) Samples were prepared of the emulsifiers and emulsifier blends to be screened for use in corn oil recovery processes. Two 100 mL solutions of each emulsifier or emulsifier blend were preparedone solution at 200 ppm and the other solution at 400 ppm (the emulsifier or emulsifier blend, in water). These emulsifier solutions were heated to ensure solubility. 30 mL of the emulsifier solutions was combined with 0.5 g of oil (dye added), vortexed, and heated to 85 C. in a water bath to form emulsifier-oil blends. The emulsifier-oil blends were centrifuged at 3000 rpm for 3 minutes, and separation was observed. An oil layer formed on top of a water layer, and the amount of oil separated can be measured. The color of the water layer for each sample is a good indication of the de-emulsification ability of the emulsifiers used. Results for several of the emulsifiers and emulsifier blends are shown in FIG. 8. On the far left of FIG. 8, the emulsifier used was alkyl polyglycosides (Glucopon 425, from BASF). Second from the left of FIG. 8, the emulsifier used was alkyl polyglycosides (Glucopon 425, from BASF) with 2% silica. In the center of FIG. 8, the emulsifier used was commercial polysorbate chemistry (i.e. Polysorbate 80). Second from the right of FIG. 8, the emulsifier blend used was 49% alkyl polyglycosides (Glucopon 425, from BASF), 49% poly(ethylene glycol) mono oleate (PEG mono oleate), and 2% hydrophilic silica. On the far right of FIG. 8, the emulsifier blend used was 44% alkyl polyglycosides (Glucopon 425, from BASF), 44% PEG mono oleate, 10% canola oil, and 2 hydrophilic silica. All the samples shows in FIG. 8 showed volumetrically similar amounts of oil separated out of the emulsifier-oil blends (as prepared according to this example). Table 4, below, lists the emulsifiers and emulsifier blends prepared according to this example.

(74) TABLE-US-00004 TABLE 4 Emulsifiers and Emulsifier Blends Tested for De-emulsification Functionality Sample No. Emulsifier or Emulsifier Blend 1 Polysorbate Chemistry (Commercial additive) 2 Alkyl polyglycosides (Glucopon 425) 3 Glucopon 425 + 2% hydrophilic silica 4 85% Glucopon 425 + 15% lecithin 5 83% Glucopon 425 + 15% lecithin + 2% hydrophilic silica 6 75% Glucopon 425 + 15% lecithin + 8% Canola Oil + 2% hydrophilic silica 7 Glucopon 425 + 10% Soy methyl ester + 2% hydrophilic silica 8 Glucopon 425 + 10% Canola Oil + 2% hydrophilic silica 9 49% Glucopon 425 + 49% PEG Mono oleate + 2% hydrophilic silica 10 44% Glucopon 425 + 44% PEG Mono oleate 10% Canola Oil + 2% hydrophilic silica 11 44% Glucopon 425 + 44% PEG Mono dioleate 10% Canola Oil + 2% hydrophilic silica 12 44% Glucopon 425 + 44% PEG Mono tallate 10% Canola Oil + 2% hydrophilic silica 13 83% Glucopon 425 + 15% ethylene oxide/propylene oxide block copolymer (PLURONIC brand L 61, from BASF) + 2% hydrophilic silica

Example 16: Oil Recovery Using Lecithin Mixture Comprising Ethoxylated Monoglycerides and Propylene Glycol

(75) In addition to Example 12, there are other high HLB surfactants that can be added to lecithin to produce a composition useful as an additive in an oil recovery process. A lecithin cosurfactant blend was prepared by mixing 56% by weight lecithin (ADM), 30% by weight ethoxylated monoglycerides (BASF), 11% by weight propylene glycol, and 3% by weight soybean oil, which mixing took place at 50 C. under constant stirring for between 30 minutes and 60 minutes, thus producing an amber, transparent lecithin cosurfactant blend. The blend may be used as an emulsifier.

Example 17. Oil Recovery Using Lecithin Mixture Comprising Ethoxylated Monoglycerides and Silica

(76) The blend from Example 16 was blended with 3% hydrophilic silica. This new blend was compared with the blend from Example 16 as a corn oil recovery additive. The additive concentration was 300 ppm based on a total weight of 40 g of CDS syrup (condensed distillers soluble syrup from an ethanol plant) for the three samples containing additive (Polysorbate 80, the blend of Example 16, the blend of Example 17). The samples were well mixed and placed in a water bath at 85 C. for 20 minutes. The samples were removed from the bath and centrifuged at 4500 rpm for 4 minutes. The oil recovery from the samples containing additive were compared to the control (no additive).

(77) The vial on the far left of FIG. 9 labeled control has no additive and has a small oil layer on the top. The vial second from the left of FIG. 9 labeled polysorbate chemistry has the additive Polysorbate 80 (polyoxyethylene (20) sorbitan monooleate) and has a small oil layer on the top. The vial third from the left of FIG. 9 labeled Example 16 has the additive from Example 16 (56% by weight lecithin (ADM), 30% by weight ethoxylated monoglycerides (BASF), 11% by weight propylene glycol, and 3% by weight soybean oil) and has a greater amount of corn oil separated as a top layer compared to either the vial labeled control or the vial labeled polysorbate chemistry. The vial on the far right of FIG. 9 labeled Example 17 contains the additive from Example 17 (the blend of Example 16, blended with 3% hydrophilic silica) and has a greater amount of corn oil separated as a top layer compared to any of the vial labeled control, the vial labeled polysorbate chemistry, and the vial labeled Example 16.

Example 18: Oil Recovery Using Lecithin Mixture Comprising PEG 400 Monooleate and Propylene Glycol

(78) In addition to Examples 12 and 16, there are other high HLB surfactants that can be added to lecithin to produce a composition useful as an additive in the oil recovery process. A lecithin cosurfactant blend was prepared by mixing 56% by weight lecithin (ADM), 30% by weight PEG 400 monooleate (BASF), 11% by weight propylene glycol, and 3% by weight soybean oil, which mixing took place at 50 C. under constant stirring for between 30 minutes and 60 minutes, thus producing an amber, transparent lecithin cosurfactant blend. The blend may be used as an emulsifier.

Example 19: Oil Recovery Using Lecithin Mixture Comprising PEG 400 Monooleate, Propylene Glycol, and Silica

(79) The blend from Example 18 was blended with 3% hydrophilic silica. This new blend was compared with the blend from Example 18 as a corn oil recovery additive. The additive concentration was 300 ppm based on a total weight of 40 g of CDS syrup (condensed distillers soluble syrup from an ethanol plant) for the three samples containing additive (Polysorbate 80, the blend of Example 16, the blend of Example 17). The samples were well mixed and placed in a water bath at 85 C. for 20 minutes. The samples were removed from the bath and centrifuged at 4500 rpm for 4 minutes. The oil recovery from the samples containing additive were compared to the control (no additive).

(80) The vial on the far left of FIG. 10 labeled control has no additive and has a small oil layer on the top. The vial second from the left of FIG. 10 labeled polysorbate chemistry has the additive Polysorbate 80 (polyoxyethylene (20) sorbitan monooleate) and has a small oil layer on the top. The vial third from the left of FIG. 10 labeled Example 18 has the additive from Example 18 (56% by weight lecithin (ADM), 30% by weight PEG 400 monooleate (BASF), 11% by weight propylene glycol, and 3% by weight soybean oil) and has a greater amount of corn oil separated as a top layer compared to either the vial labeled control or the vial labeled polysorbate chemistry. The vial on the far right of FIG. 10 labeled Example 19 contains the additive from Example 19 (the blend of Example 18, blended with 3% hydrophilic silica) and has a greater amount of corn oil separated as a top layer compared to any of the vial labeled control, the vial labeled polysorbate chemistry, and the vial labeled Example 18.

(81) The present invention has been described with reference to certain examples. However, it will be recognized by those of ordinary skill in the art that various substitutions, modifications, or combinations of any of the examples may be made without departing from the spirit and scope of the invention. Thus, the invention is not limited by the description of the examples, but rather by the appended claims as originally filed.