Antibacterial Cellulose Hydrogels and Preparation Method therefor

Abstract

The disclosure provides a preparation method for a novel biocompatible antibacterial bacterial cellulose Fmoc-F hydrogel, belonging to the technical field of biomedical gels. The preparation method is characterized by comprising the following steps of (1) preparing a bacterial cellulose (BC) homogenate, (2) mixing the bacterial cellulose with an Fmoc-F solution, and preparing the bacterial cellulose/Fmoc-F antibacterial hydrogel by means of in-situ chemical crosslinking. The preparation method has the advantages of rapid gelling reaction, simple reaction system, high gel plasticity and the like. Because of the bacterial cellulose, the antibacterial hydrogel prepared in the disclosure has high mechanical strength. Meanwhile, compared with traditional micro-molecular antibacterial materials, the bacterial cellulose/Fmoc-F antibacterial hydrogels prepared in the disclosure have broad-spectrum antibacterial effects and good biocompatibility.

Claims

1. A preparation method for an antibacterial BC-Fmoc-F hydrogel, comprising the following steps: (1) preparing a bacterial cellulose homogenate: crushing bacterial cellulose membranes with tissue homogenizer by means of mechanical homogenization so as to obtain the bacterial cellulose homogenate, centrifuging the obtained homogenate at 10000 rpm/min for 10 min to remove excessive water and the bacterial cellulose homogenate with a concentration of 10 mg/ml (dry weight) is obtained; (2) preparing a bacterial cellulose/Fmoc-L-phenylalanine hydrogel: at room temperature, weighing 120 mg of Fmoc-F precisely by analytic balance into 50 ml centrifuge tube, adding 4 ml of PB solution into each centrifuge tube for dissolution, adding 4 ml, 8 ml, 12 ml and 16 ml of the BC homogenate into the centrifuge tubes respectively, and then making up to 20 ml with the PB solution; shaking spirally for 1 min, then heating the mixture at 80 C. for 30 min in a water bath kettle, and then placing the mixture at room temperature for 6 h to obtain the BC-Fomc-F hydrogel, wherein the concentration of the Fmoc-F is 6 mg/ml, and the concentrations of the BC are respectively 2 mg/ml, 4 mg/ml, 6 mg/ml and 8 mg/ml.

2. The preparation method for the BC-Fmoc-F hydrogel according to claim 1, wherein the concentration of the bacterial cellulose suspension is 10 mg/ml.

3. The preparation method for the BC-Fmoc-F hydrogel according to claim 1, wherein the average length of the fiber of the bacterial cellulose suspension is 10-100 microns.

4. The preparation method for the BC-Fmoc-F hydrogel according to claim 1, wherein the concentrations of the BC are respectively 2 mg/ml, 4 mg/ml, 6 mg/ml and 8 mg/ml.

Description

BRIEF DESCRIPTION OF DRAWINGS

[0018] FIG. 1 shows an effect graph of the antibacterial BC-Fomc-F hydrogels prepared in the present disclosure.

[0019] FIG. 2 shows an experimental effect graph of the antibacterial BC-Fomc-F hydrogels on the inhibition zones of Bacillus subtilis and Staphylococcus aureus. (BC-0, BC-2, BC-4, BC-6 and BC-8 represent that the final concentrations of the BC are 0 mg/ml, 2 mg/ml, 4 mg/ml, 6 mg/ml and 8 mg/ml respectively.)

[0020] FIG. 3 shows a change-to-frequency graph of storage modulus (G) and loss modulus (G) of the antibacterial BC-Fomc-F hydrogels of the disclosure measured by rheometer. (BC-0, BC-2, BC-4, BC-6 and BC-8 represent the change-to-frequency graph of storage modulus (G) and loss modulus (G).)

DETAILED DESCRIPTION

[0021] In order to better understand the present disclosure, the following embodiments are provided to further illustrate the present disclosure but not to limit the present disclosure. Based on the embodiments, any other embodiment made by those skilled in the art without making any creative work shall fall within the scope of protection of the present disclosure.

[0022] A preparation method for an antibacterial BC-Fomc-F hydrogel, comprising the following steps:

[0023] (1) preparing a bacterial cellulose homogenate: crushing bacterial cellulose membranes by means of mechanical homogenization so as to obtain the bacterial cellulose homogenate, centrifuging the obtained homogenate for 10 min to remove excessive water and the bacterial cellulose homogenate with a concentration of 10 mg/ml (dry weight) is obtained;

[0024] (2) preparing the bacterial cellulose/Fmoc-L-phenylalanine (BC-Fomc-F) hydrogel: at room temperature, weighing 120 mg of Fmoc-F precisely by analytic balance into 50 ml centrifuge tube, adding 4 ml of PB solution into each centrifuge tube for dissolution, adding 4 ml, 8 ml, 12 ml and 16 ml of the BC homogenate into the centrifuge tubes respectively, and then making up to 20 ml with the PB solution; shaking spirally for 1 min, then heating the mixture at 80 C. for 30 min in a water bath kettle, and then placing the mixture at room temperature for 6 h to obtain the BC-Fomc-F hydrogel, wherein the concentration of the obtained Fmoc-F is 6 mg/ml, and the concentrations of the BC are respectively 2 mg/ml, 4 mg/ml, 6 mg/ml and 8 mg/ml.

Embodiment 1

[0025] A tissue homogenizer is used to crush bacterial cellulose membranes by means of mechanical homogenization so as to obtain the bacterial cellulose homogenate, then the obtained homogenate is centrifuged at 10000 rpm/min for 10 min to remove excessive water, and the bacterial cellulose homogenate with a concentration of 10 mg/ml (dry weight) is obtained.

[0026] At room temperature, 120 mg of Fmoc-F is precisely weighed out by an analytic balance into 50 ml centrifuge tube, 4 ml of PB solution is added into the centrifuge tube for dissolution, 4 ml of the BC homogenate is added into the centrifuge tube, and then the centrifuge tube is made up to 20 ml with the PB solution; the solution is shaked spirally for 1 min, then heated at 80 C. for 30 min in a water bath kettle and then placed at room temperature for 6 h to obtain the BC-Fomc-F hydrogel, wherein the concentration of the obtained Fmoc-F is 6 mg/ml, and the concentration of the BC is 2 mg/ml.

Embodiment 2

[0027] A tissue homogenizer is used to crush bacterial cellulose membranes by means of a mechanical homogenization so as to obtain the bacterial cellulose homogenate, then the obtained homogenate is centrifuged at 10000 rpm/min for 10 min to remove excessive water, and the bacterial cellulose homogenate with a concentration of 10 mg/ml (dry weight) is obtained.

[0028] At room temperature, 120 mg of Fmoc-F is precisely weighed out using an analytic balance into 50 ml centrifuge tube, 4 ml of PB solution is added into the centrifuge tube for dissolution, 8 ml of the BC homogenate is added into the centrifuge tube, and then the centrifuge tube is made up to 20 ml with the PB solution; the solution is shaked spirally for 1 min, then heated at 80 C. for 30 min in a water bath kettle and then placed at room temperature for 6 h to obtain the BC-Fomc-F hydrogel, wherein the concentration of the obtained Fmoc-F is 6 mg/ml, and the concentration of the BC is 4 mg/ml.

Embodiment 3

[0029] A tissue homogenizer is used to crush bacterial cellulose membranes by means of mechanical homogenization so as to obtain the bacterial cellulose homogenate, then the obtained homogenate is centrifuged at 10000 rpm/min for 10 min to remove excessive water, and the bacterial cellulose homogenate with a concentration of 10 mg/ml (dry weight) is obtained.

[0030] At room temperature, 120 mg of Fmoc-F is precisely weighed out using an analytic balance into 50 ml centrifuge tube, 4 ml of PB solution is added into the centrifuge tube for dissolution, 12 ml of the BC homogenate is added into the centrifuge tubes, and then the centrifuge tube is made up to 20 ml with the PB solution; the solution is shaked spirally for 1 min, then heated at 80 C. for 30 min in a water bath kettle and then placed at room temperature for 6 h to obtain the BC-Fomc-F hydrogel, wherein the concentration of the obtained Fmoc-F is 6 mg/ml, and the concentration of the BC is 6 mg/ml.

Embodiment 4

[0031] A tissue homogenizer is used to crush bacterial cellulose membranes by means of mechanical homogenization so as to obtain the bacterial cellulose homogenate, then the obtained homogenate is centrifuged at 10000 rpm/min for 10 min to remove excessive water, and the bacterial cellulose homogenate with a concentration of 10 mg/ml (dry weight) is obtained.

[0032] At room temperature, 120 mg of Fmoc-F is precisely weighed out using an analytic balance into 50 ml centrifuge tube, 4 ml of PB solution is added into the centrifuge tube for dissolution, 16 ml of the BC homogenate is added into the centrifuge tube, and then the centrifuge tube is made up to 20 ml with the PB solution; the solution is shaked spirally for 1 min, then heated at 80 C. for 30 min in a water bath kettle and then placed at room temperature for 6 h to obtain the BC-Fomc-F hydrogel, wherein the concentration of the obtained Fmoc-F is 8 mg/ml, and the concentration of the BC is 6 mg/ml.