COMPOSITION FOR RELIEVING STRESS AND ENHANCING IMMUNITY AND PREPARATION METHOD THEREOF

Abstract

The invention belongs to the technical field of health food and particularly relates to a composition for relieving stress and enhancing immunity and a preparation method thereof. The composition for relieving stress and enhancing immunity is calculated in parts by weight, 100-300 parts of a radix curcuma extract, 100-250 parts of a sea cucumber extract, 100-250 parts of a chlorella extract, 50-150 parts of a cranberry extract, 100-300 parts of a ginseng extract, 80-150 parts of a coconut oil extract and 100-200 parts of a palm oil extract. The composition can significantly enhance the body's immunity, relieve mental stress and improve the level of human health. In addition, the composition of the present invention is suitable for long-term use and has a small adverse reaction.

Claims

1. A composition for relieving stress and enhancing immunity, characterized in, the composition for relieving stress and enhancing immunity is calculated in parts by weight, 100-300 parts of a turmeric extract, 100-250 parts of a sea cucumber extract, 100-250 parts of a chlorella extract, 50-150 parts of a cranberry extract, 100-300 parts of a ginseng extract, 80-150 parts of a coconut oil extract, and 100-200 parts of a palm oil extract.

2. The composition for relieving stress and enhancing immunity according to claim 1, characterized in, the composition for relieving stress and enhancing immunity is calculated in parts by weight, further comprising 50-100 parts of lecithin and 50-80 parts of 5-hydroxytryptophan.

3. The composition for relieving stress and enhancing immunity according to claim 1, characterized in, the composition for relieving stress and enhancing immunity is calculated in parts by weight, 150-250 parts of a turmeric extract, 100-200 parts of a sea cucumber extract, 150-200 parts of a chlorella extract, 80-100 parts of a cranberry extract, 150-250 parts of a ginseng extract, 100-150 parts of a coconut oil extract, 150-200 parts of a palm oil extract, 50-100 parts of lecithin and 50-80 parts of 5-hydroxytryptophan.

4. The composition for relieving stress and enhancing immunity according to claim 2, characterized in, the composition for relieving stress and enhancing immunity is calculated in parts by weight, 150-250 parts of a turmeric extract, 100-200 parts of a sea cucumber extract, 150-200 parts of a chlorella extract, 80-100 parts of a cranberry extract, 150-250 parts of a ginseng extract, 100-150 parts of a coconut oil extract, 150-200 parts of a palm oil extract, 50-100 parts of lecithin and 50-80 parts of 5-hydroxytryptophan.

5. A method for preparing a composition for relieving stress and enhancing immunity according to claim 1, characterized in, mixing a turmeric extract, a sea cucumber extract, a chlorella extract, a cranberry extract, a ginseng extract, a coconut oil extract, and a palm oil extract in proportion according to the above ratio, then adding formula-proportioned lecithin and 5-hydroxytryptophan and mixing uniformly to obtain a target composition for relieving stress and enhancing immunity.

6. A method for preparing a composition for relieving stress and enhancing immunity according to claim 5, characterized in, the turmeric extract is prepared by pulverizing a turmeric medicinal material, extracting the turmeric medicinal material with a mixed solution of 50%-95% lower alcohol solution and n-hexane, the extraction time is 1-3 hours, the extraction temperature is 50 C.-85 C., and the extraction times are 2-3 times; collecting a concentrated extract, adding a 80%-90% lower alcohol solution having a volume of 0.2-0.4 times of the concentrated extract, stirring uniformly and allowing to stand for 12-24 hours, centrifuging and collecting crystals; washing the crystals again with a 80%-95% lower alcohol solution, and centrifuging to obtain crystals; drying the crystals at a low temperature to obtain a turmeric extract; The sea cucumber extract is prepared by washing a sea cucumber with cold water, adding cold purified water at 4 C. to the sea cucumber and sealing, and soaking in cold storage at 4 C. for 48 hours; then homogenizing, freezing at 20 C. until completely frozen, then thawing at room temperature, centrifuging at 4 C. and collecting a supernatant and a precipitate; filtering the supernatant through a sterile membrane with a pore size of 0.25 m, and storing a filtrate at 4 C. for use; freezing and thawing the resulting precipitate again by adding water, centrifuging and collecting a supernatant; blending the two supernatants and concentrating under reduced pressure, filtering and lyophilizing a concentrated sea cucumber extract to obtain a sea cucumber extract; The chlorella extract is prepared by pulverizing a chlorella, dispersing uniformly with water having a weight of 10-12 times of the chlorella to obtain a dispersion; centrifuging and separating the dispersion, taking a supernatant, filtering, decolorizing and lyophilizing to obtain a chlorella extract.

7. A method for preparing a composition for relieving stress and enhancing immunity according to claim 5, characterized in, the cranberry extract is prepared by pulverizing a cranberry, extracting with an acidic methanol, extracting at 80-100 C. for 10-20 minutes, and then drying into a powder; The ginseng extract is prepared by pulverizing a ginseng, soaking in water, vacuum distilling, stopping distillation when a volume of a distillate is half of the amount of added water, and collecting the distillate; and then continuously adding water to a liquid, vacuum distilling, stopping distillation when a volume of a distillate is half of the amount of added water, and collecting the distillate; blending the two distillates to obtain a ginseng extract.

8. A method for preparing a composition for relieving stress and enhancing immunity according to claim 5, characterized in, the coconut oil extract is prepared by repeatedly extracting a natural coconut oil by alcohol, and lyophilizing an extract to obtain a coconut oil extract; The palm oil extract is prepared by repeatedly extracting a natural palm oil by alcohol and lyophilizing an extract to obtain a palm oil extract.

9. A formulation for relieving stress and enhancing immunity, characterized in, the composition according to claim 1 is prepared as an active ingredient together with pharmaceutically acceptable adjuvants to obtain a forming agent; wherein, a content of the active ingredient is 16 wt %-40 wt %.

10. A formulation for relieving stress and enhancing immunity, characterized in, the composition according to claim 2 is prepared as an active ingredient together with pharmaceutically acceptable adjuvants to obtain a forming agent; wherein, a content of the active ingredient is 16 wt %-40 wt %.

11. A formulation for relieving stress and enhancing immunity, characterized in, the composition according to claim 3 is prepared as an active ingredient together with pharmaceutically acceptable adjuvants to obtain a forming agent; wherein, a content of the active ingredient is 16 wt %-40 wt %.

12. A formulation for relieving stress and enhancing immunity, characterized in, the composition according to claim 4 is prepared as an active ingredient together with pharmaceutically acceptable adjuvants to obtain a forming agent; wherein, a content of the active ingredient is 16 wt %-40 wt %.

13. A formulation for relieving stress and enhancing immunity, characterized in, the composition according to claim 5 is prepared as an active ingredient together with pharmaceutically acceptable adjuvants to obtain a forming agent; wherein, a content of the active ingredient is 16 wt %-40 wt %.

14. The formulation for relieving stress and enhancing immunity according to claim 1, characterized in, a dosage form of the formulation is a tablet, a capsule, a granule, a pill or a powder.

Description

DESCRIPTION OF THE EMBODIMENTS

[0037] The technical solutions of the present invention are further described below in conjunction with the embodiments. The described embodiments are only a part of the embodiments of the present invention, but the scope of protection of the present invention is not limited thereto, and any changes or substitutions based on the present invention belong to the scope of protection of the present invention.

[0038] The composition for relieving stress and enhancing immunity according to the present invention comprises in parts by weight, 100-300 parts of a turmeric extract, 100-250 parts of a sea cucumber extract, 100-250 parts of a chlorella extract, 50-150 parts of a cranberry extract, 100-300 parts of a ginseng extract, 80-150 parts of a coconut oil extract, and 100-200 parts of a palm oil extract.

[0039] The raw material further comprises 50-100 parts by weight of lecithin and 50-80 parts by weight of 5-hydroxytryptophan.

[0040] The composition for relieving stress and enhancing immunity comprises in parts by weight, 150-250 parts of a turmeric extract, 100-200 parts of a sea cucumber extract, 150-200 parts of a chlorella extract, 80-100 parts of a bilberry extract, 150-250 parts of a ginseng extract, 100-150 parts of a coconut oil extract, 150-200 parts of a palm oil extract, 50-100 parts of lecithin, and 50-80 parts of 5-hydroxytryptophan.

[0041] The method for preparing a composition for relieving stress and enhancing immunity according to the present invention, specifically includes:

[0042] The preparation of the turmeric extract comprises the steps of: pulverizing a turmeric medicinal material, extracting the turmeric medicinal material with a mixed solution of 50%-95% lower alcohol solution and n-hexane, the extraction time is 1-3 hours, the extraction temperature is 50 C.-85 C., and the extraction times are 2-3 times; collecting a concentrated extract, and adding a 80%-90% lower alcohol solution having a volume of 0.2-0.4 times of the concentrate, stirring uniformly and allowing to stand for 12-24 hours, centrifuging, and collecting crystals; washing the crystals again with a 80%-95% lower alcohol solution, and centrifuging to obtain crystals; drying the crystals at a low temperature, pulverizing, and sieving to obtain a turmeric extract;

[0043] The preparation of the sea cucumber extract comprises the steps of: washing a sea cucumber with cold water, adding cold purified water to the sea cucumber and sealing, a solid-liquid ratio of 1:5, and soaking in cold storage at 4 C. for 48 hours; then homogenizing the soaked sea cucumber using a homogenizer, and freezing at 20 C. until completely frozen, then thawing at room temperature, centrifuging at 4 C. and collecting a supernatant and a precipitate; filtering the supernatant through a sterile membrane with a pore size of 0.25 m, and storing a filtrate at 4 C. for use; freezing and thawing the resulting precipitate again by adding water, centrifuging and collecting a supernatant; blending the two supernatants and concentrating under reduced pressure, and filtering the concentrated sea cucumber extract through a sterile membrane with a pore size of 0.25 m and lyophilizing the filtrate in a lyophilizer to a water content of less than 3% to obtain a sea cucumber extract;

[0044] The preparation of the chlorella extract comprises the steps of: pulverizing a chlorella, dispersing uniformly with water having a weight of 10-12 times of the chlorella, sterilizing at 121 C. for 30 minutes, and cooling to room temperature to obtain a dispersion; centrifuging and separating the dispersion, taking a supernatant and filtering with diatomaceous earth; placing in a cold storage at 4 C. for 48 h, filtering again with diatomaceous earth, decolorizing with 200 mesh activated carbon for 30 min, filtering through a 0.22 m filterable membrane, lyophilizing the filtrate in a lyophilizer to a water content of less than 3% to obtain a chlorella extract.

[0045] The preparation of the cranberry extract comprises the steps of: pulverizing the cranberry, extracting with acidic methanol, extracting at 80-100 C. for 10-20 minutes, and then drying into a powder;

[0046] The preparation of the ginseng extract comprises the steps of: dicing a ginseng, refluxing and extracting 3 times with 75% ethanol having a volume of 4 times, blending the extracts, concentrating the ethanol to a syrupy thick liquid, adding ethanol with a volume percentage concentration of 95%, making the alcohol content reach to 80% (in weight percent), placing for 24 h, filtering and concentrating to a syrupy thick paste to obtain a ginseng extract.

[0047] The preparation of the coconut oil extract comprises the following steps of: adding 80% methanol to a natural coconut oil, extracting for 20 min, centrifuging at 5000 r/min for 30 min, collecting a supernatant, repeating the extraction 3 times, and blending the supernatants, removing the methanol solvent by rotary evaporation at 35 C. and lyophilizing to obtain a coconut oil extract.

[0048] The preparation of the palm oil extract comprises the following steps of: adding 80% methanol to a natural palm oil, extracting for 20 min, centrifuging at 5000 r/min for 30 min, collecting a supernatant, repeating the extraction 3 times, and blending the supernatants, removing the methanol solvent by rotary evaporation at 35 C. and lyophilized to give a palm oil extract.

[0049] The formulation of the composition for relieving stress and enhancing immunity according to the present invention is prepared by adding pharmaceutically acceptable adjuvants to the composition for relieving stress and enhancing immunity to prepare tablets, capsules, granules, pills and powder.

[0050] The use of the composition for relieving stress and enhancing immunity according to the present invention is the use of the composition for relieving stress and enhancing immunity in preparing a drug, food and health food for relieving human body stress and enhancing immunity.

[0051] The present invention is further described below with specific implementation embodiments:

Embodiment 1

[0052] 100 g turmeric extract, 100 g sea cucumber extract, 100 g chlorella extract, 50 g cranberry extract, 100 g ginseng extract, 80 g coconut oil extract, and 100 g palm oil extract were weighed, mixed the components uniformly, then, added 50 g lecithin and 50 g 5-hydroxytryptophan, and mixed uniformly to obtain a target composition for relieving stress and enhancing immunity.

Embodiment 2

[0053] 300 g turmeric extract, 250 g sea cucumber extract, 250 g chlorella extract, 150 g cranberry extract, 300 g ginseng extract, 150 g coconut oil extract, and 200 g palm oil extract were weighed, mixed the components uniformly, then, added 100 g lecithin and 80 g 5-hydroxytryptophan, and mixed uniformly to obtain a target composition for relieving stress and enhancing immunity.

Embodiment 3

[0054] 200 g turmeric extract, 175 g sea cucumber extract, 175 g chlorella extract, 100 g cranberry extract, 200 g ginseng extract, 115 g coconut oil extract, and 150 g palm oil extract were weighed, mixed the components uniformly, then, added 75 g lecithin and 65 g 5-hydroxytryptophan, and mixed uniformly to obtain a target composition for relieving stress and enhancing immunity.

Embodiment 4

[0055] 100 g turmeric extract, 250 g sea cucumber extract, 175 g chlorella extract, 100 g cranberry extract, 200 g ginseng extract, 150 g coconut oil extract, and 100 g palm oil extract were weighed, mixed the components uniformly, then, added 100 g lecithin and 80 g 5-hydroxytryptophan, and mixed uniformly to obtain a target composition for relieving stress and enhancing immunity.

Embodiment 5

[0056] 300 g turmeric extract, 100 g sea cucumber extract, 175 g chlorella extract, 50 g cranberry extract, 100 g ginseng extract, 115 g coconut oil extract, and 150 g palm oil extract were weighed, mixed the components uniformly, then, added 75 g lecithin and 50 g 5-hydroxytryptophan, and mixed uniformly to obtain a target composition for relieving stress and enhancing immunity.

Embodiment 6

[0057] 200 g turmeric extract, 100 g sea cucumber extract, 250 g chlorella extract, 150 g cranberry extract, 300 g ginseng extract, 80 g coconut oil extract, and 200 g palm oil extract were weighed, mixed the components uniformly, then, added 50 g lecithin and 50 g 5-hydroxytryptophan, and mixed uniformly to obtain a target composition for relieving stress and enhancing immunity.

Embodiment 7

[0058] 100 g turmeric extract, 250 g sea cucumber extract, 175 g chlorella extract, 50 g cranberry extract, 300 g ginseng extract, 115 g coconut oil extract, and 100 g palm oil extract were weighed, mixed the components uniformly, then, added 100 g lecithin and 75 g 5-hydroxytryptophan, and mixed uniformly to obtain a target composition for relieving stress and enhancing immunity.

Embodiment 8

[0059] 200 g turmeric extract, 100 g sea cucumber extract, 250 g chlorella extract, 100 g cranberry extract, 100 g ginseng extract, 150 g coconut oil extract, and 200 g palm oil extract were weighed, mixed the components uniformly, then, added 75 g lecithin and 80 g 5-hydroxytryptophan, and mixed uniformly to obtain a target composition for relieving stress and enhancing immunity.

Embodiment 9

[0060] 300 g turmeric extract, 250 g sea cucumber extract, 175 g chlorella extract, 50 g cranberry extract, 300 g ginseng extract, 150 g coconut oil extract, and 100 g palm oil extract were weighed, mixed the components uniformly, then, added 100 g lecithin and 80 g 5-hydroxytryptophan, and mixed uniformly to obtain a target composition for relieving stress and enhancing immunity.

Embodiment 10

[0061] Added pharmaceutically acceptable adjuvants into the composition for relieving stress and enhancing immunity prepared in Embodiment 1 to prepare a tablet, wherein, a content of the active ingredient was 40% by weight.

Embodiment 11

[0062] Added pharmaceutically acceptable adjuvants into the composition for relieving stress and enhancing immunity prepared in Embodiment 2 to prepare a capsule, wherein, a content of the active ingredient was 20% by weight.

Embodiment 12

[0063] Added pharmaceutically acceptable adjuvants into the composition for relieving stress and enhancing immunity prepared in Embodiment 4 to prepare a granule, wherein, a content of the active ingredient was 30% by weight.

Embodiment 13

[0064] Added pharmaceutically acceptable adjuvants into the composition for relieving stress and enhancing immunity prepared in Embodiment 6 to prepare a pill, wherein, a content of the active ingredient was 25% by weight.

Embodiment 14

[0065] Added pharmaceutically acceptable adjuvants into the composition for relieving stress and enhancing immunity prepared in Embodiment 8 to prepare a powder, wherein, a content of the active ingredient was 18% by weight.

Embodiment 15

[0066] The composition for relieving stress and enhancing immunity prepared in Embodiment 2 was subjected to the following pharmacodynamic test:

[0067] Test animals: 80 clean-grade Kunming mice, half male and half female, having a weight of 18-22 g each.

[0068] Experimental reagent: the composition for relieving stress and enhancing immunity prepared in Embodiment 2 was prepared into a 6% suspension with distilled water for use.

[0069] Experimental method: the experimental animals were randomly divided into 4 groups, 20 in each group: the control group was intragastrically administered with normal saline 0.3 mL/each mouse per day, the experimental group was intragastrically administered with 0.3 mL suspension per day, the concentrations of the gastric perfusion suspensions of the 3 experimental groups were 3 g/(kg.Math.bw), 5 g/(kg.Math.bw), and 7 g/(kg.Math.bw), respectively, twice a day for 2 weeks. Each mouse was intraperitoneally injected with 1 mL of 20% chicken red blood cell suspension. After 30 minutes, the mice were sacrificed by cervical dislocation and fixed on the wax plate. The skin of the abdominal wall was cut from the median, 2 mL of normal saline was injected through the abdominal cavity, and the rat plate was rotated for 1 min to fully mix the normal saline and peritoneal fluid, and then aspirated 1 mL of the peritoneal washing solution, which were then averaged to drip on 2 sheets of slides, placed in an enamel box with wet gauze, and incubated in a 37 C. incubator for 30 min. After incubation, the slides were removed and rinsed in normal saline to remove the unpatched cells. After drying, it was fixed in a 1:1 acetone methanol solution for 20 min and dyed using the Giemsa dye solution for 3 min, then rinsed with distilled water, dried, and counted the macrophages under a microscope. Calculated the phagocytosis rate and the phagocytosis index using the formula.

Phagocytosis rate (%)=macrophages that phagocytose chicken red blood cells/counted macrophages100%, phagocytosis index=total number of the swallowed chicken red blood cells/counted macrophages.

[0070] The results are shown in Table 1.

TABLE-US-00001 TABLE 1 Effect of composition for relieving stress and enhancing immunity prepared by Embodiment 2 on phagocytosis of mouse peritoneal macrophages(X s) phagocytosis phagocytosis groups numbers rate (%) index Control group 20 39.3 9.5 0.52 0.23 Low-dose 20 48.4 10.1 1.37 0.31 experimental group 3 g/(kg .Math. bw) Medium-dose 20 59.8 9.5 1.86 0.29 experimental group 5 g/(kg .Math. bw) High-dose 20 68.7 11.6 1.63 0.43 experimental group 7 g/(kg .Math. bw) Note: compared with the control, P < 0.05.

[0071] The experimental results show that the low, medium and high dose groups of the suspension of the composition for relieving stress and enhancing immunity prepared in Embodiment 2 can effectively increase the phagocytosis rate and phagocytosis index of mouse macrophages, indicating that the obtained compositions for relieving stress and enhancing immunity can increase the phagocytic activity of phagocytic cells and enhance the phagocytosis of the mononuclear phagocytic system.

Embodiment 16

[0072] Experiments with compositions for relieving stress and enhancing immunity prepared in Embodiment 1, Embodiment 3, Embodiment 4, Embodiment 5, Embodiment 6, Embodiment 7, Embodiment 8, and Embodiment 9, respectively, were carried out, methods were same as shown in Embodiment 15, the results all showed that the compositions for relieving stress and enhancing immunity of the present invention can enhance the phagocytic function of mouse peritoneal macrophages.