NON-ELICITED DEDIFFERENTIATED LAVANDULA ANGUSTIFOLIA PLANT CELLS, EXTRACTS THEREOF AND COSMETIC USES THEREOF

Abstract

The present invention relates to non-elicited dedifferentiated plant cells of a plant of the species Lavandula angustifolia, or extracts thereof, and also to the cosmetic composition comprising same, to the uses thereof and to cosmetic treatment processes comprising the application of said composition to the skin for improving and/or reinforcing the barrier function, and also for improving the moisturization of the skin.

Claims

1. Non-elicited dedifferentiated plant cells of a plant of the species Lavandula angustifolia, or extracts thereof.

2. The cells, or extracts thereof, as claimed in claim 1, characterized in that said extracts are chosen from aqueous and organic extracts, or extracts obtained by mixing water with at least one organic extraction solvent that is miscible with water in all proportions said extracts optionally being in the form of dry extracts.

3. The cells, or extracts thereof, as claimed in claim 1, characterized in that said extracts of non-elicited dedifferentiated cells of a plant of the species Lavandula angustifolia are chosen from: aqueous extracts of the intracellular medium, aqueous-alcoholic extracts of the intracellular medium, or organic extracts of the intracellular medium; said extracts optionally being in the form of dry extracts; or aqueous extracts of insoluble constituents of said cells, aqueous-alcoholic extracts of insoluble constituents of said cells, or organic extracts of insoluble constituents of said cells; said extracts optionally being in the form of dry extracts; said insoluble constituents of said cells being chosen from insoluble intracellular constituents, pectocellulose walls, cell membranes, and mixtures thereof.

4. The cells, or extracts thereof, as claimed in claim 1, characterized in that they are obtained from plant material obtained from part(s) of a plant of the species Lavandula angustifolia, said plant part(s) being one or more whole organs of the plant chosen from the leaves, the stalks, the flowers, the petals, the sepals, the seeds or the roots, or one or more fragments of said organ(s) of the plant cultivated in vivo or wild.

5. The cells, or extracts thereof, as claimed in claim 1, characterized in that they are obtained from plant part(s) chosen from the leaves or fragment(s) of leaves of the plant Lavandula angustifolia.

6. The cells, or extracts thereof, as claimed in claim 1, characterized in that they are obtained via a process comprising the following steps: i. providing one or more parts of a plant of the species Lavandula angustifolia; ii. cultivating said plant part(s) provided in step i. in a culture medium comprising at least one plant hormone, so as to generate dedifferentiated cells; and iii. recovering the dedifferentiated cells obtained at the end of step ii.; iv. optionally, extracting the dedifferentiated cells recovered in step iii.; said process not comprising a step of eliciting said dedifferentiated cells.

7. The cells, or extracts thereof, as claimed in claim 6, characterized in that said culture medium in step ii. is an aqueous culture medium comprising: at least one plant hormone such as 1-naphthaleneacetic acid, kinetin and mixtures thereof; and at least one salt optionally in hydrated form chosen from NH.sub.4NO.sub.3; KNO.sub.3; CaCl.sub.2 MgSO.sub.4; KH.sub.2PO.sub.4; MnSO4 ; ZnSO.sub.4; KI; Na.sub.2MoO.sub.4; CuSO.sub.4; Na.sub.2EDTA; FeSO.sub.4 ; and mixtures thereof; and at least one carbon source; and optionally at least one compound chosen from myoinositol, nicotinic acid, pyridoxine HCl, thiamine HCl, and mixtures thereof; and optionally polyvinylpyrrolidone.

8. The cells, or extracts thereof, as claimed in claim 1, characterized in that they are obtained by cultivation of plant part(s) from the species Lavandula angustifolia, in an aqueous culture medium comprising at least NH.sub.4NO.sub.3; KNO.sub.3; CaCl.sub.2.2H.sub.2O; MgSO.sub.4; KH.sub.2PO.sub.4; MnSO4.4H.sub.2O; ZnSO.sub.4.7H.sub.2O; KI; Na.sub.2MoO.sub.4.2H.sub.2O; CuSO.sub.4.5H.sub.2O; Na.sub.2EDTA.2H.sub.2O; FeSO.sub.4.7H.sub.2O; myoinositol; nicotinic acid; pyridoxine HCl; thiamine HCl; naphthaleneacetic acid; kinetin; sucrose, and optionally polyvinylpyrrolidone.

9. A cosmetic composition comprising, in a physiologically acceptable medium, said cells and/or extracts thereof as defined in claim 1.

10. The composition as claimed in claim 9, in which said dedifferentiated plant cells, and/or extracts thereof, are used in an amount representing from 0.01% to 40% by weight of solids relative to the total weight of the composition containing them.

11. A cosmetic treatment process comprising the application of the composition as defined in claim 9 to the skin for improving and/or reinforcing the cutaneous barrier function of the skin.

12. A cosmetic treatment process comprising the application of the composition as defined in claim 9 to the skin for improving the moisturization of the skin, for preventing and/or treating roughness or the microrelief and/or for improving the radiance of the complexion and/or for improving the suppleness of the skin.

13. A cosmetic treatment process comprising the application of the composition as defined in claim 9 to the skin for improving and/or reinforcing the protection of the skin against external attack.

14. A cosmetic treatment process comprising the application of the composition as defined in claim 9 to the skin for treating the cosmetic signs of skin dryness.

15. A cosmetic treatment process comprising applying to the skin non-elicited dedifferentiated plant cells of a plant of the species Lavandula angustifolia, or extracts thereof, as defined in claim 1, for improving and/or reinforcing the cutaneous barrier function of the skin.

16. A cosmetic treatment process comprising applying to the skin non-elicited dedifferentiated plant cells of a plant of the species Lavandula angustifolia, or extracts thereof, as defined in claim 1, for improving and/or reinforcing the protection of the skin against external attack.

17. A cosmetic treatment process comprising applying to the skin non-elicited dedifferentiated plant cells of a plant of the species Lavandula angustifolia, or extracts thereof, as defined in claim 1, for improving the moisturization of the skin, for preventing and/or treating roughness or the microrelief and/or for improving the radiance of the complexion and/or for improving the suppleness of the skin.

18. A cosmetic treatment process comprising applying to the skin non-elicited dedifferentiated plant cells of a plant of the species Lavandula angustifolia, or extracts thereof, as defined in claim 1, for treating the cosmetic signs of skin dryness.

19. A cosmetic treatment process comprising the application of the composition as defined in claim 10 to the skin for improving and/or reinforcing the cutaneous barrier function of the skin.

20. A cosmetic treatment process comprising the application of the composition as defined in claim 10 to the skin for improving the moisturization of the skin, for preventing and/or treating roughness or the microrelief and/or for improving the radiance of the complexion and/or for improving the suppleness of the skin.

Description

FIGURES

[0192] [FIG. 1] HPLC chromatogram of the extract of non-elicited dedifferentiated plant cells of the species Lavandula angustifolia obtained according to Example 1a (according to the invention) versus the extract of UV-elicited dedifferentiated plant cells of the species Lavandula angustifolia obtained according to example 2 (outside the invention).

EXAMPLES

Example 1

[0193] Example 1a—Production of an aqueous extract of the intracellular medium of non-elicited dedifferentiated cells of Lavandula angustifolia (cultivated in Drôme, France)—according to the invention

[0194] Collection and then decontamination of the aerial parts in calcium hypochlorite (50 g/l containing 60% of active chlorine or 40 g/l) for 30 minutes. Successive rinsing in three baths of sterile osmosed water (5 minutes/bath).

[0195] Chopping of the aerial parts into explants, collecting only the leaves.

[0196] The leaves are cultivated on the culture medium shown in table 1 below on agar in light and darkness.

TABLE-US-00001 TABLE 1 COMPOSITION OF THE CULTURE MEDIUM mg/l NH.sub.4NO.sub.3 1650  KNO.sub.3 1900  CaCl.sub.2•2H.sub.2O 440 MgSO.sub.4   180.8 KH.sub.2PO.sub.4 170 MnSO.sub.4•4H.sub.2O   22.3 ZnSO.sub.4•7H.sub.2O    8.6 KI    0.83 Na.sub.2MoO.sub.4•2H.sub.2O    0.25 CuSO.sub.4•5H.sub.2O     0.025 Na.sub.2 EDTA•2H.sub.2O   37.3 FeSO.sub.4•7H.sub.2O   27.8 Myoinositol 100 Nicotinic acid    0.5 Pyridoxine HCl (B.sub.6)    0.5 Thiamine HCl (B.sub.1)    0.1 PVP (polyvinylpyrrolidone) 200 Naphthaleneacetic acid  1 Kinetin     Sucrose 20 000   Water qs 1 L

[0197] Following the successive subculturings in the presence of culture medium, dedifferentiated plant cells which can be cultivated in a fermenter were obtained. The parameters used for the controlling the cultivation in a bioreactor are the following: [0198] T°: 27° C.; [0199] Aeration: pO.sub.2 30%, regulated by the sterile air entering and/or via stirring while avoiding any shear stress on the cells. [0200] Stirring: 150 rpm.

[0201] The batchwise production lasts about 10 days. The culture medium obtained is then separated from the dedifferentiated cells by filtration through a gauze with a porosity of 50 μm.

[0202] On conclusion of the filtration step, said cells obtained are milled using a high-pressure homogenizer (2000 bar) in the presence of water in a [cells/water] mass ratio of 1/1. The particles in suspension are then removed by filtration at 10 000×G for 30 minutes at 4° C. followed by filtration of the supernatant using a 0.7 μm Whatman cellulose filter, so as to obtain an aqueous extract of the intracellular medium.

[0203] The aqueous extract thus obtained is dried by lyophilization under the following conditions: step of freezing the sample at −40° C., followed by sublimation by placing under vacuum (<1 mbar) at 20° C., and then secondary desiccation achieved at a lower pressure of 100 μbar by means of elimination of air injection into the system; leading to a dry extract.

[0204] Example 1b—Production of an alcoholic extract of the intracellular medium of non-elicited dedifferentiated cells of Lavandula angustifolia—according to the invention

[0205] On conclusion of the filtration step through a 50 μm gauze in example 1, said cells obtained are milled using a high-pressure homogenizer (2000 bar) in the presence of ethanol: the [cells/solvent] ratio is 1/1. The particles in suspension are then removed by filtration at 10 000×G for 30 minutes at 4° C. followed by filtration of the supernatant using a 0.7 μm Whatman cellulose filter, so as to obtain an alcoholic extract of the intracellular medium.

[0206] The extract thus obtained is dried in two steps: [0207] by concentrating on a rotary evaporator at 50° C. followed by redissolving in water; and then [0208] by lyophilization under the following conditions: step of freezing the sample at −40° C., followed by sublimation by placing under vacuum (<1 mbar) at 20° C., and then secondary desiccation achieved at a lower pressure of 100 μbar by means of elimination of air injection into the system; leading to a dry extract.

[0209] Example 1c—Production of a hydrolyzate of pectocellulose walls and of membranes of non-elicited dedifferentiated cells of Lavandula angustifolia—according to the invention

[0210] On conclusion of the filtration step through a 50 μm gauze in example 1, said cells obtained are milled using a high-pressure homogenizer (2000 bar) in the presence of water in a [cells/water] mass ratio of 1/1. The particles in suspension or debris are then recovered by centrifugation at 10 000×G for 30 minutes at 4° C.

[0211] A first step of enzymatic hydrolysis of the pellet obtained is performed with carbohydrases, this step enabling the hydrolysis of the cellulose compounds: [0212] suspension of the wall debris in a solution buffered at pH 4 (50 mM citrate/phosphate buffer) to a debris/buffered solution proportion of 10/100. [0213] hydrolysis of the wall debris by adding an enzymatic solution of carbohydrases (Viscozyme L from Novozyme) in an enzymatic solution/buffered debris suspension proportion ranging from 2.5/100. This mixture is placed at the optimum working temperature of the carbohydrases, at 50° C., and with stirring at 150 rpm for a period of 90 minutes.

[0214] A second step of protein hydrolysis with proteases is then performed: [0215] adjustment of the pH of the reaction mixture to the preferential pH for proteases, at 8, by simple addition of a concentrated basic solution such as potassium hydroxide. [0216] hydrolysis of the wall debris by adding an enzymatic solution of protease (Alcalase 2.4 L from Novozyme) in an enzymatic solution/buffered debris suspension proportion ranging from 2.5/100. This mixture is placed at the optimum working temperature of the proteases, at 50° C., and with stirring at 150 rpm for a period of 90 minutes.

[0217] To complete these hydrolysis steps, the hydrolysis product undergoes inactivation of the enzymes at 90° C. for 15 minutes. followed by separation of the remaining particles in suspension from the hydrolysis product obtained from the preceding two hydrolysis steps by centrifugation (10 000×G, 4° C., 30 minutes).

[0218] In order to concentrate and to store the supernatant obtained from the centrifugation, it is dried by lyophilization under the following conditions: step of freezing the sample at −40° C., followed by sublimation by placing under vacuum (<1 mbar) at 20° C., and then secondary desiccation achieved at a lower pressure of 100 μbar by means of elimination of air injection into the system.

[0219] The product obtained is called the cell wall hydrolyzate; it is rich in saccharide compounds.

Example 2—Production of an Aqueous Extract of UV-Elicited Dedifferentiated cells of Lavandula angustifolia—Outside the Invention

[0220] During their cultivation in a production medium (cf. Table 1, culture medium composition), the cell lines of Lavandula angustifolia are elicited 10 days after being inoculated, with UV light (280-400 nm) using four Philips CLEO performance sunlamps (40-0-14/2.6) placed at a distance of 50 cm, in direct lighting above the cells for 15 hours.

[0221] This elicitation means obviously does not form any impurities in the cell culture. At the end of cultivation, i.e. 24 hours after eliciting, the plant cells are filtered through a gauze with a porosity of 50 μm to remove the remaining culture medium. On conclusion of the filtration step, a fresh biomass is thus obtained; said cells obtained are milled in a high-pressure homogenizer in the presence of water at 2000 bar so as to extract the intracellular medium of the cells. The extract thus obtained is dried by lyophilization under the following conditions: step of freezing the sample at −40° C., followed by sublimation by placing under vacuum (<1 mbar) at 20° C., and then secondary desiccation achieved at a lower pressure of 100 μbar by means of elimination of air injection into the system.

Example 3

[0222] Example 3a: analysis of the UPLC chromatographic profile of the extracts obtained according to example 1a (according to the invention) and according to example 2 (outside the invention)

[0223] A) Materials and Methods

[0224] In order to perform the pseudo-quantitative analysis of the extracts 1a and 2, they are prepared at identical concentrations and the solutions are doped with a standard absorbing in the UV range: caffeine.

[0225] The first step consists in dissolving the samples in water to give solutions at 5 g/L. The solutions thus generated are heated gently and subjected to ultrasound for 30 minutes.

[0226] The second step consists in preparing a solution of caffeine in water at a concentration of 0.17 g/L.

[0227] The final step consists in mixing 1.8 mL of sample solution (cell extracts 1a or 2) with 0.2 mL of the caffeine solution, homogenizing the mixtures and then filtering through a 0.45 μm filter and then a 0.2 μm filter.

[0228] The analysis is performed on an Acquity UPLC Additol H-Class system (Waters) comprising a diode array detector, a Corona detector (CAD) and a single quadrupole mass spectrometer.

[0229] Chromatography System [0230] Acquity UPLC BEH Shield RP18 column [0231] Length 50 mm [0232] Inside diameter 2.1 mm [0233] Column volume 0 ml [0234] Particle diameter 1.8 μm [0235] Mobile phase: A=Water 0.1% HCOOH; B=ACN (acetonitrile) 0.1% HCOOH [0236] Flow rate=0.5 mL/min [0237] Injection volume 2 μL [0238] Temperatures: Tcolumn=30° C.; Tsamples=20°C.

[0239] Gradient 1 is shown in Table 2 below.

TABLE-US-00002 TABLE 2 T (min.) % A % B P (psi) 0 99 10 1 99 10 5 70 30 6.5 0 100 7.5 0 100 8 99 10 10 99 10

[0240] Detection [0241] Diode array detector (DAD): the chromatogram is recorded over the 200-700 nm range. [0242] Corona charged aerosol detector (CAD): the pressure is set at P=35.1 psi which corresponds to a nitrogen flow rate set at D=1.21 bar. [0243] Mass spectrometer (MS): coupling of HPLC to mass spectrometry is performed with a single quadrupole mass spectrometer equipped with an electrospray ionization (ESI) source; the mass spectrometer operates simultaneously in positive and negative ionization mode over the 100-2000 amu mass range.

[0244] B) Results

[0245] The two chromatograms of the extracts 1a and 2 were superposed (see FIG. 1).

[0246] Among the 23 target compounds: [0247] 1 compound becomes depleted when the cells are elicited with UV, [0248] 6 compounds are not affected by the elicitation, [0249] 11 compounds become enriched when the cells are elicited with UV, [0250] 5 compounds are newly synthesized (not detected in the non-elicited sample, at the limits of the method).

[0251] Thus, the two extracts 1a and 2 differ from each other in their composition.

[0252] Example 3b: evaluation of the effect of the extract of non-elicited dedifferentiated cells of Lavandula angustifolia on barrier function/moisturization markers obtained according to Example 1a (according to the invention) versus an extract outside the invention of UV-elicited dedifferentiated cells of Lavandula angustifolia obtained according to example 2 (outside the invention)

[0253] A) Materials and Methods

[0254] Cytotoxicity

[0255] Normal human epidermal keratinocytes were seeded in 96-well culture plates and then cultivated at 37° C. and 5% CO2 in culture medium for 24 hours. The medium was then replaced with culture medium containing or not containing (control) the test compounds (8 concentrations tested), and the cells were then incubated for 24 hours. All the conditions were performed in n=2. At the end of the incubation, the cell viability was measured by a standard test of measuring the mitochondrial activity with Alamar Blue®.

[0256] Analysis of the Expression of the Genes Associated with the Barrier Function/Moisturization by RT-qPCT

[0257] Normal human epidermal keratinocytes (NHEK) were seeded in 48-well culture plates and then cultivated in culture medium for 3 days at 37° C and 5% CO2 with renewal of the culture medium after the first 24 hours of cultivation. At the end of the incubation, the culture medium was replaced with test medium (supplemented with 1.5 mM CaCl2) not containing or containing (control) the test compounds, and the cells were then incubated for 24 hours. All the conditions were performed in n=2.

[0258] At the end of the treatments, the culture media were removed and the cells were rinsed twice with PBS (w/o CaCl2, w/o MgCl2). The total RNA was then isolated using a magnetic bead extraction kit and according to the supplier's recommendations (MagMAXTM-96 Total RNA Isolation Kit, Ambion). The RNA quantification and the quality control thereof were analyzed by means of Labchip GX (Perkin Elmer).

[0259] The expression of the selected transcripts was analyzed by quantitative PCR in two steps. First, the cDNAs were reverse-transcribed from the RNAs using the Quantitect® Reverse transcription kit (Qiagen) and according to the supplier's recommendations. The quantitative PCR experiments were then performed using a LightCycler® 480 Real-Time PCR System in a 384-well plate (Roche) and according to the SYBR®Green (Roche) incorporation technique. The primers used are shown in Table 2 below.

TABLE-US-00003 TABLE 3 Gene Ref. Genes Abbreviation ID Qiagen Epidermal cohesion Keratinocyte Aquaporin 3 AQP3 360 QT00212996 differentiation Moisturization Barrier Cornified layer Cornifelin CNFN 84518 QT00208453 function assembly Small proline- SPRR1A 6698 QT02448782 rich protein 1A GAG synthesis Hyaluronic acid HAS3 3038 QT00014903 synthase 3 Epidermal renewal Heparin-binding HBEGF 1839 QT01667610 EGF-like growth factor

[0260] B) Results

TABLE-US-00004 TABLE 4 Level of RNA expression following treatment of the Level of RNA expression NHEKs with the extract following treatment of the obtained according to NHEKs with the extract example 1a (extract obtained according to according to the example 2 (extract outside Markers invention) at 0.10 mg/l the invention) at 0.10 mg/l HBEGF 10.39 5.85 CNFN 10.09 4.52 SPRR1A 3.94 1.80 HAS3 2.87 1.55

[0261] The extract of non-elicited cells obtained according to Example 1a (extract according to the invention) markedly stimulated the expression of transcripts involved in assembly of the cornified layer (SPRR1A, CNFN), keratinocyte differentiation (AQP3) and epidermal renewal (HBEGF) compared with the extract of UV-elicited cells according to example 2 (extract outside the invention).

[0262] Thus, the extract 1a of non-elicited dedifferentiated cells from Lavandula angustifolia according to the invention proves to be particularly effective in preventing and treating dehydrated skin and improving and reinforcing the barrier function.

Example 4—Evaluation of the Moisturizing Potential on Isolated Stratum Corneum by Measurement with a Corneometer

[0263] A test was performed to evaluate the moisturizing potential of the extracts of the invention formulated in a vehicle (80%/20% water/n-propanol) in an amount of 5% by weight relative to the total weight of the composition.

[0264] The technique makes it possible to measure the dielectric capacitance of the stratum corneum (SC), which depends on the mean dielectric permittivity value of the tissue. The dielectric permittivity varies greatly with the amount of water contained in the SC.

[0265] The SC samples are conditioned at 75% relative humidity and at 25° C. before/during the measurements and the treatment. The capacitance measurement is performed using a Corneometer™ (Courage & Khazaka, Germany).

[0266] The test extracts, extracts 1a, 1b and 1c according to the invention or a moisturizing active agent such as glycerol, are dissolved in a water/n-propanol mixture (80/20) and the solution is deposited onto the SC at a rate of 10 μL/cm.sup.2followed by air-drying for a total duration of 4 hours.

[0267] A measurement is taken at T0, before the treatment. and a measurement Ttreat(4h) is taken after total drying of the treatment.

[0268] Each treatment is systematically compared with its control (vehicle) and with its T0.

[0269] Using at least two different batches of SC, four to five SC samples are measured per treatment.

[0270] The variation in the corneometer signal (HCM) after treatment is calculated first for each SC sample: DHCMi=HCMi(Ttreat)−HCMi(T0). The mean of the DHCMi(vehicle) variations is then calculated for the control samples (treated with the vehicle): this mean value is subtracted from all the DHCMi(active agent) and DHCMi(positive control) variations to correct for the systematic bias.

[0271] The following are measured for each sample i:

[0272] For the vehicle (control): DHCMi(veh)=HCMiveh(Ttreat)−HCMiveh(T0)

[0273] For the active agent: DHCMiactive agent=HCMiactive agent(Ttreat)−HCMiactive agent(T0)

[0274] For the positive control (glycerol): DHCMipositive control=HCMipositive control(Ttreat)−HCMipositive control(T0).

[0275] To correct for the systematic bias associated with the vehicle, the corrected value DHCMicorr. active agent is considered for the active agent according to: DHCMicorr. active agent=DHCMiactive agent−M(veh)

[0276] in which M(veh) corresponds to the mean of the DHCMi(veh) variations observed on the n vehicle control samples:

[00001]$\begin{array}{cc}\frac{1}{n}\underset{i=1}{\overset{n}{.Math.}}\mathrm{DHCMi}\left(\mathrm{veh}\right)& \left[\mathrm{Math}1\right]\end{array}$

[0277] To correct for the systematic bias associated with the vehicle, the corrected value DHCMicorr. positive control is considered for the positive control according to: DHCMicorr. positive control=DHCMipositive control−M(veh)

[0278] where M(veh) is as defined previously.

[0279] The DHCMicorr. positive control and DHCMicorr. active agent values are then normalized according to the following calculation:

% norm=[(DHCMicorr. positive control)/(M(positive control)−M(veh))]+100

norm=[(DHCMicorr. active agent)/(M(positive control)−M(veh))]+100

[0280] where M(veh) is as defined previously;

[0281] where M(positive control) corresponds to the mean of the DHCMipositive control variations observed on the n positive control samples:

[00002]$\begin{array}{cc}\frac{1}{n}\underset{i=1}{\overset{n}{.Math.}}\mathrm{DHCMi}\left(\mathrm{positive}\mathrm{control}\right)& \left[\mathrm{Math}2\right]\end{array}$

[0282] The % norm values that were obtained are reported in the table below for the extracts according to the invention tested at 5%, compared with glycerol at 5%:

TABLE-US-00005 TABLE 5 Extract 1a Extract 1b Extract 1c Products tested according to according to according to in a water/n- Vehicle 80% Glycerol the invention the invention the invention propanol water/20% at 5% by at 5% by at 5% by at 5% by mixture n-propanol weight weight weight weight % norm 0% 100% 116% 115% 190%

[0283] This test shows that the dielectric capacitance of the stratum corneum (SC) obtained with extract 1a or 1b is similar to that obtained with glycerol at the same concentration. In addition, the dielectric capacitance of the stratum corneum (SC) obtained with extract 1c is much better than that obtained with glycerol at the same concentration.

[0284] Unexpectedly, extracts 1a, 1b and 1c according to the invention allow good moisturization of the stratum corneum and thus of the skin. This moisturizing effect proves to be either similar to (extracts 1a and 1b) or greater than (extract 1c) that of glycerol.

Example 5—Cosmetic Composition

[0285] The following composition was prepared.

TABLE-US-00006 TABLE 6 Ingredients Amount (m/m) Extract of non-elicited dedifferentiated 0.01 cells from Lavandula angustifolia obtained according to Example 1a Xanthan gum 0.5 Preserving agents qs Water qs 100

[0286] The above composition was applied to the skin to reinforce the barrier function and/or to moisturize the skin.