COMPOSITION FOR RESTORATION OF TISSUE INCLUDING BIODEGRADABLE POLYMERIC COPOLYMER

Abstract

The present invention provides a composition for restoration of tissue including a biodegradable polymeric copolymer which is obtained by polymerizing a hydrophobic biodegradable polymer and a hydrophilic biodegradable polymer. The composition of the present invention has excellent tissue restoration effects because of high collagen production rate without occurrence of nodules even when injected directly into the dermis, unlike the conventional composition for restoration of tissue.

Claims

1. A composition for restoration of tissue comprising a biodegradable polymeric copolymer obtained by polymerizing a hydrophobic biodegradable polymer and a hydrophilic biodegradable polymer.

2. The composition for restoration of tissue according to claim 1, wherein the biodegradable polymeric copolymer is obtained by polymerizing a hydrophobic biodegradable polymer and a hydrophilic biodegradable polymer in a weight ratio of 10:20 to 10:50.

3. The composition for restoration of tissue according to claim 1, wherein the hydrophobic biodegradable polymer is polycaprolactone.

4. The composition for restoration of tissue according to claim 1, wherein the hydrophilic biodegradable polymer is methoxy polyethyleneglycol or polyethyleneglycol.

5. The composition for restoration of tissue according to claim 1, wherein the copolymer has a particle diameter of 50 nm to 300 nm.

6. The composition for restoration of tissue according to claim 1, wherein the copolymer has a number average molecular weight of 2,500 to 5,000 g/mol.

7. The composition for restoration of tissue according to claim 1, wherein the copolymer is included in an amount of 0.1 to 30% (w/w) in an aqueous solution.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0022] The above and other objects, features and other advantages of the present invention will be more clearly understood from the following detailed description taken in conjunction with the accompanying drawings, in which:

[0023] FIG. 1 is vial photographs showing an injectable formulation including a product of Comparative Example 1, and compositions including products of Comparative Example 2 and Example, respectively;

[0024] FIG. 2 is a graph showing results of analyzing particle diameters of nanoparticles of methoxy polyethyleneglycol-polycaprolactone copolymer according to Example of the present invention;

[0025] FIG. 3 is photographs showing results of comparing properties after storage of the injection compositions containing the products of Comparative Example 2 and Example, respectively, at 37° C. for 24 hours;

[0026] FIG. 4 is photographs showing a site of SD white rat filled with the product of Example, that is, the injectable formulation of the present invention after injecting the same in Experimental Example 1 of the present invention;

[0027] FIG. 5 shows histological test results for the sites injected with the products of Comparative Example 1 and Example, respectively, in Experimental Example 1 of the present invention;

[0028] FIG. 6 is photographs showing collagen formed in the skin tissues at the injection sites of Comparative Example 1 and Example, respectively; and

[0029] FIG. 7 is a graph quantitatively illustrating the amounts of collagen formed in the skin tissues at the injection sites of Comparative Example 1 and Example, respectively.

DETAILED DESCRIPTION OF THE INVENTION

[0030] Hereinafter, the present invention will be described in detail by the following examples and experimental examples.

[0031] However, the following examples and experimental examples are only illustrative of the present invention, and the content of the present invention is not limited thereto.

COMPARATIVE EXAMPLE 1

Polycaprolactone-Carboxymethylcellulose Copolymer Microparticles

[0032] ELLANSE M (manufacturer: AQTIS Medical, vendor: JW Foreign Pharmaceuticals) in an aqueous solution, which includes polycaprolactone polymer microparticles and carboxymethyl cellulose in a weight ratio of 3:7, is an injectable formulation (“injection”) consisting of polycaprolactone particles having an average particle diameter of about 50 μm.

COMPARATIVE EXAMPLE 2

Formulation of Methoxy Polyethyleneglycol-Polycaprolactone Copolymer Particles

[0033] After polymerizing 10 g of monomethoxy polyethyleneglycol (molecular weight: 1,000 g/mol) and 20 g of ε-caprolactone monomer in a weight ratio of 10:20 at 120° C. for 12 hours under 100 μl of stannous octoate catalyst, the product was cooled at room temperature to obtain a methoxy polyethyleneglycol-polycaprolactone copolymer having a molecular weight of 3,000 g/mol.

[0034] Distilled water at room temperature was added to the above prepared copolymer, followed by stirring and mixing the same so as to form nanoparticles by the polymer through self-assembly, and thereby obtaining a solution of copolymer at 30% (w/w) in an aqueous solution.

Example, Formulation of Methoxy Polyethyleneglycol-Polycaprolactone Copolymer Nanoparticles

[0035] After polymerizing 21 g of monomethoxy polyethyleneglycol (molecular weight: 1,000 g/mol) and 10 g of ε-caprolactone monomer in a weight ratio of 21:10 at a temperature of 100° C. for 12 hours under 100 μl of stannous octoate catalyst, the product was cooled at room temperature to obtain a methoxy polyethyleneglycol-polycaprolactone copolymer having a molecular weight of 3,100 g/mol. Thereafter, distilled water at room temperature was added to the prepared copolymer, followed by mixing the same so as to form nanoparticles by the copolymer through self-assembly, and thereby obtaining a solution of copolymer at 30% (w/w) in an aqueous solution. The copolymer particles contained in the aqueous solution had an average particle diameter of 162 nm.

EXPERIMENTAL EXAMPLE 1

Comparison Between Formulations of Methoxy Polyethyleneglycol-Polycaprolactone Copolymer Nanoparticles Depending on Temperature

[0036] After storing the products of Example and Comparative Example 2, respectively, at 37° C. for 24 hours, properties depending on temperature were compared, and results thereof are shown in FIG. 3. As a result of the experiment, the formulation of methoxy polyethyleneglycol-polycaprolactone copolymer nanoparticles with a high proportion of hydrophobic polymer according to Comparative Example 2 showed formation of precipitates due to recombination of particles, and irregular particles were formed. However, the formulation of methoxy polyethyleneglycol-polycaprolactone copolymer nanoparticles of Example did not produce precipitates.

EXPERIMENTAL EXAMPLE 2

Assessment of Tissue Restoration Effects of the Aqueous Solution of Methoxy Polyethyleneglycol-Polycaprolactone Copolymer Nanoparticles

[0037] In order to assess the tissue restoration effects of the present invention, an experiment was performed as follows.

[0038] An injection including the product of Comparative Example 1, which is 0.1 ml of microparticle composition, was used as a control group, while an injection including the product of Example, which is 0.1 ml of methoxy polyethyleneglycol-polycaprolactone copolymer nanoparticle composition, was used as an experimental group.

[0039] For an experiment of injecting the injection, SD white rat (SD Rat, Lion Bio Co., Ltd., Korea) was used. Each rat was anesthetized with isoflurane gas (Hana Pharm Co., Ltd., Korea) before injection. Once the sedation was clearly observed, the animal was placed on a surgical table in a supine position, and the claws were tied up with elastic strips. A 2×1 cm surface of the rat's scalp was depilated.

[0040] The panning technique was used to inject the material into the scalp at a uniform pressure using a disposable insulin syringe 26G. An injection part was selected at one place on the head, followed by injection (FIG. 4).

[0041] Three (3) months after the injection, more than 2×1 cm.sup.2 of the injection site was dissected to completely remove a part in which all nodular sites from the skin having nodules formed thereon and even to the upper layer of the superficial muscle are included.

[0042] Three (3) months after the injection, scalp tissues at the injection site of the SD white rat are shown in FIG. 5.

[0043] As a result of the experiment, when visually observing the extracted skin tissue 3 months after application of the test material, the nodules due to the injection were detected in the scalp tissue of the control group while the nodules due to the injection were not observed in the experimental group.

[0044] Further, the injection sites of the experimental group and the control group were subjected to histological examination. Particularly, the skin of the injection site of the SD white rat was extracted and subjected to the histological examination. As a result of the examination, it was observed that nanoparticles were mutually bound and existed at the injection site due to absorption of aqueous solution in the tissue, and further were present along with collagen. These results are shown in FIG. 6.

EXPERIMENTAL EXAMPLE 3

Quantitative Evaluation of Collagen Production

[0045] In order to quantitatively determine a change in collagen of Experimental Example 2, the scalp tissues of SD white rat at 4 weeks, 8 weeks and 12 weeks after the injection of the test material were dissected and obtained.

[0046] A weight of the scalp tissue was quantified, and a 10-fold amount of 0.5 M acetic acid solution was added, stirred at 4° C. for 18 hours and centrifuged (15,000 g, 60 minutes), in order to collect a supernatant till reaching a total volume of 100 μl. After adding 1 ml of sircol dye reagent to combine collagen with the dye reagent and centrifuging the same (10,000 g, 10 minutes), 1 ml of alkali reagent was added to the obtained precipitate, followed by measuring absorbance at 540 nm using a soluble collagen assay kit (Sircol™, Biocolor Ltd., Ireland) thus to determine a quantitative amount of collagen in each tissue, and the compared results are shown in FIG. 7.

[0047] As a result of the experiment, the control group injected with the product of Comparative Example 1 showed slight increase in collagen from 0.51 mg/g at 4 weeks to 0.6 mg/g at 8 weeks, while the amount was very little changed at 12 weeks. On the other hand, the experimental group injected with the product of Example was confirmed that the collagen was increased from 0.88 mg/g at 4 weeks to 1.22 mg/g at 12 weeks by 0.34 mg/g.