4,4-diphenylpiperidine compounds or pharmaceutically acceptable salts thereof, pharmaceutical compositions and uses thereof

Abstract

The invention belongs to the field of medicine and chemical industry and relates to a 4,4-diphenylpiperidine compound or a pharmaceutically acceptable salt thereof, a pharmaceutical composition comprising the same and uses thereof. In particular, the invention relates to a compound of Formula I or a pharmaceutically acceptable salt thereof, and to a pharmaceutical composition comprising the compound or a pharmaceutically acceptable salt thereof. In the present invention, the compound or pharmaceutically acceptable salt thereof and the pharmaceutical composition have significant activity in blocking an N-type calcium channel, and have good pharmacokinetic properties, can effectively relieve pain, and have a potential as a new medicament for prevention or treatment of pain, stroke, cerebral ischemia, alcohol addiction, alcoholism, kidney disease, addictive disorder caused by analgesic or tolerance disorder caused by analgesic. ##STR00001##

Claims

1. A compound of Formula I or a pharmaceutically acceptable salt thereof: ##STR00020## wherein: g is selected from the group consisting of methylene, carbonyl, methyleneacyl, ethylideneacyl, 1,3-propylideneacyl, 1,2-propylideneacyl, 1,4-butylideneacyl, 1,3-butylideneacyl and 1,2-butylideneacyl; R is selected from the group consisting of substituted phenyl, thienyl and benzoxazolinonyl; the substituted phenyl is substituted with one or more substituents, the substituent is selected from the group consisting of methoxy and dimethylamino.

2. The compound of Formula I or a pharmaceutically acceptable salt thereof according to claim 1, which is selected from the group consisting of the following compounds and pharmaceutically acceptable salts thereof: 6-(3-(4,4-diphenylpiperidinyl)-propionyl)benzoxazolin-2-one; 6-(3-(4,4-diphenylpiperidinyl)-acetyl)benzoxazolin-2-one; 1-(4-dimethylaminobenzyl)-4,4-diphenylpiperidine; 1-(3,4,5-trimethoxybenzyl)-4,4-diphenylpiperidine; 1-(2-methylthienyl)-4,4-diphenylpiperidine.

3. A pharmaceutical composition, comprising the compound or a pharmaceutically acceptable salt thereof according to claim 1.

4. A method of blocking or inhibiting a N-type calcium ion channel in vivo or in vitro, comprising the step of: administering to a subject in need thereof an effective amount of the compound or a pharmaceutically acceptable salt thereof according to claim 1.

5. A method for treatment of a pain, a stroke, a cerebral ischemia, an alcohol addiction, an alcoholism, a renal disease, an addictive disorder caused by an analgesic drug or a tolerable disorder caused by an analgesic drug, comprising the step of: administering to a subject in need thereof an effective amount of the compound or a pharmaceutically acceptable salt thereof according to claim 1.

6. The pharmaceutical composition according to claim 3, further comprising a pharmaceutically acceptable excipient.

7. A method of blocking or inhibiting a N-type calcium ion channel in vivo or in vitro, comprising the step of: administering to a subject in need thereof an effective amount of the pharmaceutical composition according to claim 3.

8. A method for treatment of a pain, a stroke, a cerebral ischemia, an alcohol addiction, an alcoholism, a renal disease, an addictive disorder caused by an analgesic drug or a tolerable disorder caused by an analgesic drug, comprising the step of: administering to a subject in need thereof an effective amount of the pharmaceutical composition according to claim 3.

9. The method according to claim 5, wherein, the pain is a postoperative pain, a migraine, a visceral pain or a neuropathic pain.

10. The method according to claim 5, wherein, the renal disease is an acute renal failure, a chronic renal failure or a renal insufficiency.

11. The method according to claim 8, wherein, the pain is a postoperative pain, a migraine, a visceral pain or a neuropathic pain.

12. The method according to claim 8, wherein, the renal disease is an acute renal failure, a chronic renal failure or a renal insufficiency.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) In order to make the content of the present invention more easily understood, the present invention will be further described in detail in combination with the specific embodiments and the accompanying drawings of the invention below, in which:

(2) FIG. 1 shows the concentrations of Compound (1) in plasmas of 6 rats in Test Example 4 of the present invention;

(3) FIG. 2 shows the concentrations of Compound (1) in rats' plasmas after intravenous administration and oral administration in Test Example 4 of the present invention.

SPECIFIC MODELS FOR CARRYING OUT THE INVENTION

Example 1

Synthesis of 6-(3-(4,4-diphenylpiperidinyl)-propionyl) benzoxazolin-2-one (Compound (1))

(4) ##STR00014##

(5) 1.89 g (0.01 mol) of N-benzyl-4-piperidone was weighed and dissolved in 20 mL of dry benzene, added with 20 mL of trifluoromethanesulfonic acid under stirring, and stirred for 4 hours at room temperature. The reaction liquid was poured into ice water, added with 40% sodium hydroxide aqueous solution to adjust pH to 10-12, extracted for 3 times each with 30 mL of dichloromethane. The extracts were combined and washed first with 30 mL of saturated sodium chloride solution and second with 30 mL of water, then added with an appropriate amount of anhydrous sodium sulfate and stood overnight for drying, filtered to remove the drying agent, and evaporated under reduced pressure to remove solvent and to give 3.24 g of white solid (4,4-diphenyl-1-benzylpiperidine, Compound (6)), yield 99%.

(6) 3.24 g of the 4,4-diphenyl-1-benzylpiperidine (Compound (6)) prepared above was dissolved in 100 mL of toluene, added with 20 mL of ethyl chloroformate, heated and refluxed for 8 hours reaction. After evaporating the solvent under reduced pressure, 100 mL of distilled water and 8 mL of 40% sodium hydroxide aqueous solution were added to the residue, and the reaction was performed under heating and reflux for 24 hours. The solvent was evaporated under reduced pressure, and 100 mL of distilled water was added thereto, and extraction was performed for 3 time each with 40 mL of dichloromethane. The extracts were combined and washed first with 30 mL of saturated sodium chloride solution and second with 30 mL of water, added with an appropriate amount of anhydrous sodium sulfate and stood overnight for drying, filtered to remove the drying agent, and evaporated under reduced pressure to remove solvent and to give 1.81 g of white solid, which was 4,4-diphenylpiperidine, m.p.: 148 C.-150 C., yield: 76.0%. .sup.1H-NMR (CDCl.sub.3, ppm) : 7.30-7.34 (m, 4H), 7.20-7.23 (m, 6H), 3.21 (t, 4H, J=5.32 Hz), 2.68 (t, 4H, J=5.32 Hz).

(7) 0.47 g (0.002 mol) of the 4,4-diphenylpiperidine and 0.45 g (0.002 mol) of 6-(3-chloropropionyl)benzoxolin-2-one prepared above were dissolved in 30 mL of acetone, added with 0.22 g (0.002 mol) of triethylamine, and stirred at room temperature for 24 hours. After suction filtration, the resulted solid was washed with diethyl ether and water, and dried to obtain 0.52 g of white solid (Compound (1)), m.p.: 236 C. (decomposition), yield: 61.0%. .sup.1H-NMR (DMSO-d.sub.6, ppm) : 7.79-7.81 (m, 2H), 7.10-7.34 (m, 11H), 3.12 (t, 2H, J=7.00 Hz), 2.41-2.58 (m, 10H). MS [M+H].sup.+: 427.3.

Example 2

Synthesis of 6-(3-(4,4-diphenylpiperidinyl)-acetyl)benzoxazolin-2-one (Compound (2))

(8) ##STR00015##

(9) 0.47 g (0.002 mol) of 4,4-diphenylpiperidine (prepared by referring to the method of Example 1) and 0.45 g (0.002 mol) of 6-(2-chloroacetyl)benzoxazolin-2-one were taken and dissolved in 30 mL of acetone, added with 0.22 g (0.002 mol) of triethylamine, and stirred at room temperature for 24 hours. After suction filtration, the resulted solid was washed with diethyl ether and water, and dried to obtain 0.44 g of white solid (Compound (2)), yield: 52.0%. .sup.1H-NMR (DMSO-d.sub.6, ppm) : 7.79-7.81 (m, 2H), 7.10-7.34 (m, 11H), 3.76 (s, 2H), 2.40-2.53 (m, 8H). MS[M+H].sup.+: 425.2.

Example 3

Synthesis of 1-(4-dimethylaminobenzyl)-4,4-diphenylpiperidine (Compound (3))

(10) ##STR00016##

(11) 0.47 g (0.002 mol) of 4,4-diphenylpiperidine (prepared by referring to the method of Example 1) and 0.30 g (0.002 mol) of 4-dimethylaminobenzaldehyde were weighed and dissolved in 25 mL of dry dichloromethane, stirred at room temperature for 1 hour; 0.53 g (0.0025 mol) of sodium triacetoxyborohydride was added thereto under the cooling of ice water bath, and further stirred for 30 minutes in ice water bath, and then warmed to room temperature and continuously stirred for 8 hours. The reaction mixture was added with 20 mL of dichloromethane, and washed with saturated sodium hydrogencarbonate aqueous solution, saturated sodium chloride aqueous solution and water 30 mL each. The dichloromethane layer was separated, added with an appropriate amount of anhydrous sodium sulfate and stood overnight for drying, filtered to remove drying agent, evaporated under reduced pressure to remove solvent, and separated by silica column to obtain 0.49 g of white solid (Compound (3)), m.p.: 128 C.-130 C., yield: 66.1%. .sup.1H-NMR (CDCl.sub.3, ppm) : 7.12-7.29 (m, 12H), 6.68 (d, 2H, J=8.68 Hz), 3.34 (s, 2H), 2.48-2.51 (brs, 8H). MS [M+H].sup.+: 371.0.

Example 4

Synthesis of 1-(3,4,5-trimethoxybenzyl)-4,4-diphenylpiperidine hydrochloride (Compound (4) hydrochloride)

(12) ##STR00017##

(13) 0.47 g (0.002 mol) of 4,4-diphenylpiperidine (prepared by referring to the method of Example 1) and 0.19 g (0.002 mol) of 3,4,5-trimethoxybenzaldehyde were weighed, and the synthesis was performed by referring to the method of Example 3 to give a pale yellow oily substance (Compound (4)), the salifying was performed with diethyl ether solution of hydrogen chloride to obtain 0.46 g of white solid (Compound (4) hydrochloride), m.p.: 235 C.-237 C., yield: 55.1%. .sup.1H-NMR (CDCl.sub.3, ppm) : 12.51 (br, 1H), 7.44-7.48 (t, 2H, J=7.56 Hz), 7.03-7.46 (m, 10H), 3.91 (s, 6H), 3.85 (s), 3H), 3.45 (t, 2H), 2.13 (t, 2H), 2.71 (t, 4H). MS [M+H].sup.+: 418.6.

Example 5

Synthesis of 1-(2-methylthienyl)-4,4-diphenylpiperidine (Compound (5))

(14) ##STR00018##

(15) 0.47 g (0.002 mol) of 4,4-diphenylpiperidine (prepared by referring to the method of Example 1) and 0.22 g (0.002 mol) of 2-thiophenaldehyde were weighed, and the synthesis was performed by referring to the method of Example 3 to give 0.56 g of white solid (Compound (5)), yield: 84.1%. .sup.1H-NMR (DMSO-d.sub.6, ppm) : 6.88-7.41 (m, 11H), 3.54 (s, 2H), 2.41 (m, 8H). MS [M+H].sup.+: 334.4.

Test Example 1

Analgesic Effect of the Compounds of the Present Invention

(16) 1. Experimental purposes: To determine the analgesic activity of Compound (4) hydrochloride and Compounds (1), (2), (3), (5) in a mouse acetic acid writhing model.

(17) 2. Experimental materials: Kunming mice (18-22 g), half male and half female, were provided by the Experimental Animal Center of the Academy of Military Medical Sciences.

(18) 3. Experimental methods and results:

(19) The mice were weighed and labeled, 10 mice in each group (half male and half female), the mice were divided into 7 groups, including 5 experimental groups (using Compound (4) hydrochloride, Compound (1), (2), (3), (5), respectively), positive control group (using NMED-160) and negative control group (using normal saline). Among them, NMED-160 used in the positive control group was a selective small molecule N-type calcium ion channel blocker, which had entered the phase II clinical trial and could be synthesized by referring to document.sup.[11], and the structural formula thereof is as follows:

(20) ##STR00019##

(21) The mice were intragastrically administered with drug (30 mg/kg). After 40 minutes, 0.4 mL of 0.6% (v/v) acetic acid was intraperitoneally injected. After 5 minutes, the number of mouse writhings in the subsequent 15 minutes was recorded. The inhibition rate of the drug on the acetic acid writhing in mice was calculated according to the following formula, and the analgesic effect of the drug was evaluated. The results are shown in Table 2.

(22) wherein:

(23) $\mathrm{Writhing\_Inhibition}\_\mathrm{Rate}\left(\%\right)=\frac{A-B}{A}100\%$

(24) A represents the number of writhings in the negative control group;

(25) B represents the number of writhings in the experimental groups or the positive control group.

(26) TABLE-US-00002 TABLE 2 Analgesic results Writhing inhibition Number of animals per Drug rate (%) group (mouse) NMED-160 51.75 10 Compound (1) 58.77 10 Compound (2) 54.66 10 Compound (3) 23.17 10 Compound (4) 39.68 10 hydrochloride Compound (5) 46.87 10

(27) Table 2 shows that the compound and the pharmaceutically acceptable salt thereof according to the present invention have significant analgesic effects; wherein the analgesic effects of Compounds (1) and (2) are comparable to that of NMED-160.

Test Example 2

N-Type Calcium Ion Channel Current Suppressing Activity of the Compound of the Present Invention

(28) 1. Experimental Purpose:

(29) To determine the current suppressing effects of Compound (4) hydrochloride and Compounds (1), (2), (3), (5) of the present invention on N-type calcium ion channel (.sub.1B/.sub.1b/2.sub.) transiently expressed in Xenopus oocytes.

(30) 2. Experimental Materials:

(31) Xenopus oocytes.

(32) N-type calcium ion channel cDNAs plasmid .sub.1B (GeneBank accession no. AF055477)/.sub.2 (AF286488)/.sub.1b (L06110), rabbit .sub.1C (X15539), human .sub.1A (NM000068), rat .sub.1E (NM 009782) and rat HERG (U04270).

(33) 3. Experimental Method:

(34) (1) Amplification of Different Subunit cDNAs of Calcium Channel:

(35) E. coli competent cells containing .sub.1B, .sub.2, .sub.1b plasmids (cDNAs plasmids) were separately placed in LB solution (100 mL) containing ampicillin (50 g/mL), and shaken at 37 C., 200 rpm for 12 to 17 hours, for next day use.

(36) (2) Extraction of Plasmids:

(37) 4 mL of the above culture of E. coli was centrifuged for 5 minutes in a tabletop centrifuge (12000 g), the supernatant was discarded, and the tube was placed upside down on a paper towel to absorb the remaining culture solution. 250 L of cell suspension solution was added to the above tube, vortexed or blown to fully suspend the cells, and the suspended cells were transferred to a 5 mL sterilized centrifuge tube. 250 L of cell lysis solution was added thereto and the tube was inverted 4 times to mix well. The incubation time was approximately 1 to 5 minutes. Additionally, 10 L of alkaline protease solution was added thereto and the tube was inverted 4 times to mix well, and incubation was performed for 5 minutes at room temperature. The alkaline protease was capable of inactivating nucleases and other proteins released during cell lysis that affected the quality of the isolated plasmids. Then, 350 L of neutralizing solution was added to the centrifuge tube and the tube was rapidly inverted 4 times to mix well, and the centrifuge tube was subjected to centrifugation at the maximum speed (14,000 g) for 10 minutes at room temperature.

(38) About 850 L of the clear lysate from the above centrifugation was transferred to the prepared spin-column without agitating or transferring any white precipitate along with the supernatant. Centrifugation was performed at room temperature for 1 minute at the maximum speed with centrifuge, the centrifuge tube was taken from the collection tube, the liquid in the collection tube was discarded, and the spin-column was reinserted into the collection tube. 750 L of the column washing solution previously diluted with 95% (w/w) ethanol was added thereto. Centrifugation was performed at room temperature for 1 minute at the maximum speed with centrifuge, the centrifuge tube was taken from the collection tube, the liquid in the collection tube was discarded, and the spin-column was reinserted into the collection tube. 250 L column washing solution was added, and the washing was repeated once. Centrifugation was performed at room temperature for 2 minutes at the maximum speed with centrifuge.

(39) The spin-column was transferred to a sterilized 1.5 mL centrifuge tube, which must be handled carefully without transferring the washing solution along with the spin-column. 100 L of nuclease-free water was added to elute plasmid DNA (optionally waited for 2 minutes for complete dissolution), and centrifugation was performed at the maximum speed for 1 minute at room temperature with centrifuge. After elution, the spin-column was taken out and discarded. The centrifugal liquids were combined and stored at 20 C. for later use and the concentration was measured.

(40) (3) Isolation and Culture of Xenopus oocytes:

(41) Surgical instruments were immersed in 75% (w/w) ethanol for 30 minutes and taken out to dry. Number 5 thread was sterilized in boiling water for 10 minutes.

(42) Xenopus laevis was buried in crushed ice for about 40 minutes for anesthetization. The anesthetized Xenopus laevis was taken out and placed on the tiled crushed ice in an abdomen up position, and its head and limbs were buried with crushed ice. Its lower abdomen skin was sterilized with alcohol cotton ball, then picked up (at left or right side of the middle) with a needle, and cut with a pair of ophthalmic scissors to form a small opening of about 1 cm. The muscle layer was cut in the same way (carefully not to damage any internal organs, and oocytes could be seen after cutting the muscle layer). Lobules with a size of 1 cm.sup.3 were taken out with tweezers and scissors, and placed in a pre-prepared culture dish containing OR-2 (containing penicillin), and the muscle layer and the skin layer were sutured, respectively.

(43) The oocytes were transferred into a sterilized glass tube and washed repeatedly with OR-2 solution until the residual blood was washed away. Collagenase solution was added thereto and shaken for about 1 hour, and then fresh collagenase solution was replaced and the shaking was continued for about 1 hour (most of the isolated or single cells could be seen at this time).

(44) After removing the digestive solution, the cells were washed 5-6 times with OR-2 solution, transferred to a culture dish containing ND-96 solution to select phase V mature cells and place them into ND-96 solution, and stored in a biochemical incubator for use at 1 C., and the solution was replaced once per day.

(45) (4) Injection of Calcium Channel Plasmid:

(46) Plasmids .sub.1B, .sub.2, .sub.1b were injected into Xenopus oocytes in concentration ratio of 1:1:1, and the total volume of the three plasmids injected into per cell was about 46 nL. The injected cells were placed in ND-96 medium at 18 C. for 48 hours, then the expressed current was recorded.

(47) (5) Current Record:

(48) Perfusion administration method was adopted with a flow rate of 3 mL/min and a test drug concentration of 10 M. Using two-electrode voltage clamp technique, the cell was clamped at 100 mV, stepped at 10 mV, depolarized to +60 mV, and the current was recorded.

(49) The experimental results of the N-type calcium ion channel current suppression experiment are shown in Table 3.
Current inhibition rate (%)=100(current amplitude after administrationcurrent amplitude before administration)/current amplitude before administration

(50) TABLE-US-00003 TABLE 3 Experimental results of compounds inhibiting N-type calcium ion channel currents (10 M) Drug Current inhibition rate (%) Cell number Compound (1) 70.6 3 Compound (2) 65.6 3 Compound (3) 42.3 3 Compound (4) 54.1 3 hydrochloride Compound (5) 45.3 3

(51) The above results indicate that the compounds and the pharmaceutically acceptable salt thereof according to the present invention have strong inhibitory effects on N-type calcium ion channel.

Test Example 3

Current-Inhibition Activity of the Compounds of the Present Invention in P/Q-Type Calcium Channel, Herg Channel, Sodium Channel, and Potassium Channel

(52) (1) Enzyme Digestion Linearization of P/Q-Type and Herg Channel DNAs

(53) 1. The subunits of P/Q-type calcium channel (.sub.2, .sub.1b, .sub.1B, .sub.1E, .sub.1A, .sub.1C) and Herg channel DNA were from the same source as in. Test Example 2, and the enzyme digestion was carried out according to the following system:

(54) TABLE-US-00004 Hpa I 1 L 10 MBuffer 2 L DNA(.sub.2) 1 g Sterile water added to total volume of 20 L; Xba I 1 L 10 M Buffer 2 L DNA(.sub.1b) 1 g Sterile water added to total volume of 20 L; Not I 1 L 10 M Buffer 2 L DNA(.sub.1B) 1 g Sterile water added to total volume of 20 L; Xba I 1 L 10 M Buffer 2 L DNA(.sub.1E) 1 g Sterile water added to total volume of 20 L; Xba I 1 L 10 M Buffer 2 L DNA(.sub.1A) 1 g Sterile water added to total volume of 20 L; Xba I 1 L 1 M Buffer 2 L DNA(.sub.1C) 1 g Sterile water added to total volume of 20 L; Xba I 1 L 10 M Buffer 2 L DNA(Herg) 1 g Sterile water added to total volume of 20 L;

(55) the plasmid DNAs were subjected to enzyme digestion according to the above reaction systems, and incubated at 37 C. for 1 hour.

(56) 2. DNA Purification and RNA Enzymes Removal:

(57) a. To the plasmid DNA sample that had been enzyme digested, 0.1 times volume of 10 proteinase K buffer, 0.1 times volume of 5% SDS solution, 20 mg/mL proteinase K (final concentration was 100 g/mL) were added, and incubation reaction was performed at 37 C. for 1 hour;

(58) b. The above reaction mixture was transferred to a clean 1.5 mL eppendorf tube, added with an equal volume of Tris-balanced phenol:chloroform:isoamyl alcohol (25:24:1), shaken for 1 minute, centrifuged at 12000 rpm for 2 minutes at room temperature (20-25 C.) (if the organic and inorganic phases were not sufficiently separated, centrifugation was repeated for an extended period of time);

(59) c. The upper aqueous phase was carefully transferred to another clean 1.5 mL eppendorf tube, the interfacial layer between the two phases and the organic phase were discarded;

(60) d. An equal volume of chloroform:isoamyl alcohol (24:1) was added thereto, and centrifuged at 12000 rpm for 2 minutes at room temperature (20-25 C.);

(61) e. The upper aqueous phase was carefully transferred to another clean 1.5 mL eppendorf tube, added with 0.1 times volume of 3M sodium acetate (pH 5.2) and 2.5 times volume of 95% (W/W) ethanol, shaken to mix well, and stood at 20 C. overnight (12-16 hours);

(62) f. Centrifugation was performed at 12000 rpm for 40 minutes at a low temperature (<4 C.);

(63) g. The supernatant was carefully removed without agitating the sediment, and all droplets attached to the tube wall were sucked out with a sucker;

(64) h. To the sediment obtained by the previous step, half-tube of 70% (w/w) ethanol was added, shaken to mix well, and centrifuged at 12000 rpm for 2 minutes at low temperature (<4 C.);

(65) i. The supernatant was carefully removed, and the previous step was repeated;

(66) j. The 1.5 mL eppendorf tube was kept in open state at room temperature (20-25 C.) until the residual liquid was evaporated;

(67) k. An appropriate volume of Nuclease-free water was added thereto with rinsing the tube wall thoroughly, mixed well to completely dissolve the template DNA, measured to determine the concentration value, and stored at 20 C. for later use.

(68) 3. In Vitro Transcription of Calcium Ion Channel Subunits and HERG Channel cDNA into cRNA.sup.[13,14]:

(69) The reactants were added in the following order of the reaction systems:

(70) Linearized templates of .sub.1A, .sub.1b, .sub.2, .sub.1E, .sub.1A, .sub.1C subunit and Herg channel subunit;

(71) T7 Transcription 5Buffer 20 L;

(72) rNTPs (25 mM ATP, CTP, UT, each 7.5 L+3 mM GTP, 0.6 L+nuclease-free water, 6.9 L);

(73) Linear DNA templates (5-10 g in total)+nuclease-free water 32.5 L;

(74) Ribom7 hat analog, 40 mM, 7.5 L;

(75) MixE, 10 L.

(76) a. The reaction mixture was taken out from the incubator, added with RQ1 Rnase-Free Dnase (in a ratio of 1 u enzyme per g template DNA), and the incubation reaction was performed at 37 C. for 15 minutes;

(77) b. An equal volume of water-balanced phenol:chloroform:isoamyl alcohol (25:24:1) was added thereto, shaken for 1 minute, and centrifuged at 12,000 rpm for 2 minutes at room temperature (20-25 C.) (if the organic and inorganic phases were not fully separated, the centrifugation was repeated for an extended period of time);

(78) c. The upper aqueous phase was carefully transferred to another clean 1.5 mL eppendorf tube, added with an equal volume of chloroform:isoamyl alcohol (24:1), and centrifuged at 12000 rpm for 2 minutes at room temperature (20-25 C.);

(79) d. The supernatant was carefully transferred to another clean 1.5 mL eppendorf tube, added with 0.1 times volume of 3M sodium acetate (pH 5.2) and 2.5 times volume of 95% (w/w) ethanol, shaken to mix well, and stood at 20 C. overnight (12-16 hours);

(80) e. Centrifugation was performed at 12,000 rpm for 40 minutes at low temperature (<4 C.);

(81) f. The supernatant was carefully removed without agitating the sediment, and all droplets attached to the tube wall were sucked out with a sucker;

(82) g. To the sediment obtained in the previous step, half-tube of 70% (w/w) ethanol was added;

(83) h. The supernatant was carefully removed, and the previous step was repeated;

(84) i. The 1.5 mL eppendorf tube was kept in open state at room temperature (20-25 C.) until the residual liquid was evaporated, an appropriate volume of nuclease-free water was added with rinsing the tube wall thoroughly, mixed well to completely dissolve mRNA, and stored at 70 C. for later use.

(85) 4. Injection of Xenopus oocytes:

(86) After drawing the special injection needle with two-step electrode drawing instrument, the needle was used to puncture a clean thin tissue paper in order to enlarge the tip diameter of the needle, and polished with polishing instrument to make the needle tip smooth and flat. The diameter of the needle tip was preferably 6-10 m. Prior to installing the injection needle, Light mineral oil was injected into the needle to lubricate the tube wall. Based on the concentrations of the original subunit cRNAs, the injection concentrations were adjusted to approximately 2 ng/nL and separately mixed (1:1:1). 1 L of the mixed cRNA was carefully dropped onto a clean parafilm, and the cRNA was indrawn into the injection needle by using a microinjector (Note: the contamination of RNase should be prevented; the indrawing of air into the injection needle should be prevented; both of which could affect the expression of cRNA in the oocytes). The selected healthy mature oocytes of phases V and VI were placed in a Petri dish with a mesh (which prevented the cells from sliding in the dish) at the bottom. The 3D micromanipulator was carefully adjusted so that the needle tip touched cell surface. If the cell was in good condition, a resistance would be felt when the needle tip touched the cell, and wrinkles formed by the tension on the cell membrane would be seen. The needle tip should not puncture the cell too deeply, and it was advisable to just pass the needle tip through the cell membrane. 46.5 nl of cRNA was injected into the oocyte, and the cell swelled slightly after the injection. After waiting for 30 s, the injection needle was pulled out and observed if any cell contents spilled, and if so, discarded the cell. In addition, if the needle tip was easily pierced into the cell without breakthrough feeling, it indicated that the cell was tension-free and in poor condition, meaning that the cell might not be able to clamp to the desired potential, and the cell should be discarded as well. The injected oocytes were placed in ND-96 solution and incubated for 48 hours in a biochemical incubator (18 C.) (ND-96 solution was replaced everyday), and the expressions of P/Q-type calcium ion channel current and Herg channel current were recorded separately.

(87) 5. Recording Extracellular Fluid and Intracellular Fluid of Calcium Channel Expressed by Xenopus:

(88) Extracellular fluid (mM): BaCl.sub.2 5, N-methyl-D-glucamine 50, KCl 5, HEPES 5, adjusted to pH 7.4 with methanesulfonic acid.

(89) The intracellular fluid was 3 mM KCl. Preparation of BAPTA: BAPTA was dissolved in 10 mM Hepes and the pH was adjusted to 7.2 with CsOH.

(90) (2) Primary Cultured Hippocampal Neurons

(91) 1. Culture of Hippocampal Neurons:

(92) Newborn Wistar rats were taken and sterilized with 75% (w/w) ethanol. The brain was taken under aseptic condition, and the hippocampus was peeled off and placed in an anatomical solution in an ice bath. The hippocampus was cut into tissue blocks of 1-2 mm.sup.3, digested with an anatomical solution containing 0.25% trypsin for 30 minutes at 37 C., and then the digested tissue blocks were transferred to a planting solution to terminate digestion, and a pipette with an appropriate caliber (having tip diameter of 2 mm) was used to blow and beat the cells in the planting solution so that the cells were evenly dispersed to prepare a cell suspension, and a small amount of the suspension was taken to count the cells in a trypan blue dye. An appropriate amount of planting solution was added, the cells were inoculated in a 35 mm culture dish previously coated with polylysine at a density of 110.sup.5/mL, and placed in a 10% carbon dioxide incubator at 36 C. overnight, the culture medium was replaced after 24 hours, and the planting solution was changed to 2 mL of culture solution. Hereafter, the culture solution was changed once every three days at half volume, and the cultured cells during the period of 12-15 days were used for patch clamp experiments. In order to inhibit non-neuronal hyperproliferation, an appropriate amount of cytarabine was added into the medium on the third day of culture (6 L of cytarabine stock solution was added to each dish to reach a final concentration of 3 g/mL).

(93) 2. Recording Intra-Electrode Fluid and Extracellular Fluid of Hippocampal Neuron Current:

(94) The recording fluids used for whole-cell recording were mainly intra-electrode fluid and extracellular fluid.

(95) Recording intra-electrode fluid components (mM): KCl 140, HEPES 10, EGTA 10, and the pH was adjusted to 7.2-7.4.

(96) Components of extracellular fluid (mM): NaCl 140, KCl, 5, MgCl.sub.2 1, HEPES 10, Glucose 10, CaCl.sub.2 3, and the pH was adjusted to 7.2-7.4 with NaOH. If the experiment required blocking sodium current, 1 M tetrodotoxin (TTX) could be added to the extracellular fluid. If the experiment required blocking potassium current and only CsCl could not completely block potassium current, tetraethylammonium (TEA) or 4-aminopyridine (4-AP) could be added to the intra-electrode fluid and the extracellular fluid.

(97) The measurement results are shown in Table 4.

(98) TABLE-US-00005 TABLE 4 Current inhibition results for P/Q-type calcium channel, Herg channel, sodium channel and potassium channel TTX Voltage- P/Q-type sensitive gated calcium Herg sodium potassium Drug (10 M) channel channel channel channel Compound (1) Compound (2) Compound (3) Compound (4) hydrochloride Compound (5) indicates that the compound had no effect on this channel current.

(99) The experimental results show that the compound and pharmaceutically acceptable salt thereof according to the present invention have no blocking or inhibiting effect on P/Q-type calcium channel, Herg channel, TTX-sensitive sodium channel and voltage-gated potassium channel.

(100) Therefore, the compounds and pharmaceutically acceptable salt thereof according to the present invention, are capable of specifically inhibiting an N-type calcium ion channel.

Test Example 4

Rapid Evaluation of Pharmacokinetics in Rats

(101) 1. Materials and Instruments:

(102) Compound (1) was dissolved in 25% (w/v) aqueous solution of hydroxypropyl--cyclodextrin;

(103) Megestrol: internal standard substance, purchased from the Inspection Institute of China;

(104) Hydroxypropyl--cyclodextrin: purchased from Xi'an Deli Biochemical Co., Ltd., prepared as a 25% (w/v) aqueous solution of hydroxypropyl--cyclodextrin;

(105) Acetonitrile, methanol: chromatographically pure, purchased from Fisher Company;

(106) Pure water;

(107) Adult SD rats, 180-220 g in body weight, male, purchased from the Animal Center of the Academy of Military Medical Sciences, Animal Certificate SCXK-(Army)-2012-0004;

(108) Liquid chromatography-mass spectrometer (LC-MS/MS), triple quadrupole cascade LC-MS/MS (API 5000);

(109) LDZ5-2 type centrifuge (Beijing Medical Centrifuge Factory);

(110) MicroCL21R high speed refrigeration centrifuge (Thermo Company, USA);

(111) JJ-1 type precision booster electric mixer (Changzhou Guohua Electric Co., Ltd.);

(112) XW-80A type vortex mixer (Shanghai Qingpu Huxishi Instrument Factory);

(113) Sartorius BS110S Electronic Analytical Balance (Beijing Sartorius Balance Co., Ltd.), Libror EB-330D Electronic Analytical Balance (Shimadzu, Japan).

(114) 2. Drawing Standard Curve:

(115) 1 mg/mL mother solution of Compound (1) was prepared with acetonitrile, and gradually diluted to obtain standard solutions with a series of concentrations; multiple portions of blank rat plasma were taken at 0.05 mL, separately added with 0.005 mL standard solutions of the series of concentrations and 0.3 mL of acetonitrile containing internal-standard substance megestrol (10 ng/mL), resulting in that, the final concentrations of Compound (1) were 1 ng/mL, 2 ng/mL, 5 ng/mL, 20 ng/mL, 100 ng/mL, 200 ng/mL, 500 ng/mL, 1000 ng/mL, 2000 ng/mL, respectively, then vortexed for 1 minute on a vortex mixer, centrifuged for 10 minutes (14000 g), about 0.15 mL of the supernatant was taken and placed in an injection vial, and injected 5 L into LC-MS/MS for analysis.

(116) Quality control sample: The mother solution of Compound (1) (1 mg/mL) was diluted stepwise into three standard solutions of low, medium and high concentrations. A plurality of blank rat plasma were taken at 0.05 mL, separately added with 0.005 mL standard solutions of low, medium and high concentrations and 0.3 mL of acetonitrile containing internal-standard substance megestrol (10 ng/mL), resulting in that the final concentrations of Compound (1) were 2 ng/mL, 50 ng/mL, 1600 ng/mL, respectively, which were used as quality control samples of Compound (1) (repeated 3 samples for each concentration, n=3), vortexed for 1 minute on vortex mixer, centrifuged for 10 minutes (14000 g), about 0.15 mL of the supernatant was taken and placed in injection vial, and injected 5 L into LC-MS/MS for analysis.

(117) Operation Conditions of Liquid Chromatography:

(118) Column: Kromasil 100-5 C18, Dim 502.1 mm, (Sweden);

(119) Mobile phase composition: mobile phase A was water (containing 0.1% formic acid), mobile phase B was acetonitrile (containing 10 mM ammonium acetate and 0.1% formic acid), and the gradient elution procedure was shown in Table 5.

(120) TABLE-US-00006 TABLE 5 Mobile phase gradient elution procedure Flow rate Mobile phase Mobile phase Time (min) (mL/min) A (%) B (%) 0.5 0.6 90 10 2 0.6 5 95 2.5 0.6 5 95 2.6 0.6 90 10 4 0.6 90 10

(121) MS/MS Quantitative Analysis Conditions:

(122) The ionization mode was electrospray (ESI) condition, positive ion detection;

(123) The detected m/z values of Compound (1) and the internal standard substance were 427.168 and 385.2, respectively. Selective ion (SRM) method was used for detection: the fragment ion of the m/z 427.168 was m/z 250.1, and CE was 18; and the fragment ion of the internal standard substance m/z 385.2 was m/z 267.3, and CE was 25.

(124) A standard curve was drawn for the measured peak areas relative to the concentrations of Compound (1), the linear range was 1 ng/mL to 2000 ng/mL, the lowest detection line was 1 ng/mL, and the linearity was good. The precision of the detected concentrations of the quality control samples and the accuracy between them and the true concentrations were all within 15%, indicating that the detection method was accurate and reliable.

(125) 3. Rapid Evaluation of PK in Rats:

(126) Six adult SD rats were randomly numbered, and divided into 2 groups to receive intravenous injection (i.v., 1.85 mg/kg) and oral administration (p.o., 11 mg/kg) of Compound (1), respectively; blood samples were collected at 0.1 mL before administration and 2 minutes, 5 minutes, 15 minutes, 30 minutes, 60 minutes, 2 hours, 4 hours, 7 hours and 24 hours after administration, placed in anticoagulation tubes, centrifuged for 15 minutes (3500 rpm) to separate plasma, and 0.05 mL plasma samples were accurately taken and frozen in a refrigerator at 30 C. for later tests.

(127) To the 0.05 mL rat plasma sample, 0.3 mL of acetonitrile containing the internal standard substance megestrol (100 ng/mL) and 0.005 mL of blank acetonitrile were added, vortexed for 1 minute on a vortex mixer, and centrifuged for 10 minutes (14,000 g), about 0.15 mL of the supernatant was taken and placed into an injection vial, injected 5 L to LC-MS/MS for detection and analysis by referring to the method of the above section 2. In combination with the standard curve obtained in the above section 2, the concentrations of Compound (1) in 6 rats at different time points were calculated, as shown in FIG. 1, in which, 1 # to 3 # were of i.v. administration, and 4 # to 6 # were of p.o. administration. The results of 1 # to 3 # were averaged, the results of 4 # to 6 # were averaged, and the relationship curves of the average concentrations of Compound (1) in the rats of i.v. administration and in the rats of p.o. administration against time were obtained and shown in FIG. 2. The Winnolin pharmacokinetic software was used to analyze the measured data and calculate the main pharmacokinetic parameters, including the area under curve (AUC), clearance rate (CL), apparent distribution volume (V), terminal elimination half life (t.sub.1/2) and mean residence time (MRT) for the intravenous injection, as well as the area under curve (AUC), terminal elimination half life (t.sub.1/2), mean residence time (MRT), peak concentration (C.sub.max), time to peak (T.sub.max) and biological force (F) for the oral administration. The obtained pharmacokinetic parameters are shown in Table 6.

(128) TABLE-US-00007 TABLE 6 Pharmacokinetic parameters after intravenous injection (1.85 mg/kg) and oral administration (11 mg/kg) of Compound (1) in rats (n = 3) Compound (1) Intravenous injection Oral administration Parameter Unit (1.85 mg/kg) (11 mg/kg) AUC g/L*h 750.1 98.2 1674.4 222.4 MRT h 3.87 0.18 5.6 0.46 t.sub.1/2 h 5.0 0.88 5.4 1.5 C.sub.max ng/mL 187.7 28.5 T.sub.max h 1.33 0.58 CL L/h/kg 2.38 0.29 V L/kg 12.39 3.17 F % 44.7 5.93

(129) The results of pharmacokinetic study in rats showed that the in vivo average clearance rate of Compound (1) after intravenous administration was 2.38 L/h/kg, which was slightly lower than rat liver blood flow (3.3 L/h/kg).sup.[15], suggesting that Liver metabolism had about 70% effect on the clearance of compound (1) in vivo; the stable apparent distribution volume V was 12.39 L/kg, which was much larger than the volume of rat plasma (0.03 L/kg).sup.[15] and the total water volume in rat (0.6 L/kg).sup.[15], suggesting that most of the compound was mainly distributed in extravascular tissues, and the probability of exposure in target tissues was also large; the average time to peak after oral administration was 1-2 h, suggesting that the intestinal absorption of the compound was good; as compared with the in vivo drug exposure amount after oral administration, the average bioavailability was 44.7%, the metabolic properties were good. In summary, the compound as an analgesic drug has good pharmacokinetic properties in rats, and further researches including the drug distribution in target sites of brain tissue under steady state conditions can be carried out.

Test Example 5

Evaluation of the Central Nervous System Distribution

(130) 1. Materials and Instruments:

(131) Identical to those of Test Example 4.

(132) 2. Drawing Standard Curves:

(133) This was carried out by referring to the method of section 2 in Test Example 4;

(134) In addition, the blank rat plasma was replaced with blank rat brain homogenate, and a standard curve of Compound (1) for rat brain homogenate was drawn.

(135) 3. Evaluation of Central Nervous System Distribution of Compound (1) in Plasma and Brain Homogenate of Rats:

(136) Compound (1) was prepared at a concentration of 4 mg/mL with a 25% (w/v) aqueous solution of hydroxypropyl- cyclodextrin. Three adult SD rats were randomly numbered, first injected intravenously with 0.4 mL Compound (1) solution, and then slowly instilled with the Compound (1) solution (0.4 mL/h) for 2 hours, respectively. Blood samples in volume of 0.1 mL were collected before the administration and 30 minutes, 60 minutes and 2 hours after the administration, respectively, and placed in anticoagulant tubes, centrifuged for 15 minutes (3500 rpm) to separate plasma, and 0.05 mL plasma samples were accurately taken, and frozen in a refrigerator at 30 C. for later test.

(137) After 2 hours of blood collection, the rats were executed by femoral artery bleeding, and the whole brain and spinal cord were taken, rinsed with physiological saline, placed on filter paper to suck water, and frozen in a refrigerator at 30 C. for later test.

(138) To the 0.05 mL of rat plasma sample, 0.3 mL of acetonitrile containing the internal standard substance megestrol (100 ng/mL) and 0.005 mL of blank acetonitrile were added, vortexed for 1 minute on a vortex mixer, and centrifuged for 10 minutes (14,000 g). About 0.15 mL of the supernatant was taken and placed in an injection vial, injected 5 L to LC-MS/MS for detection and analysis by referring to the method of Test Example 4. In combination with the standard curve obtained in the above section 2, the concentrations of Compound (1) in the rat plasma samples were calculated.

(139) The rat brain tissue samples and the rat spinal cord samples were weighed and added to normal saline at 1:4 (v/v) to prepare a homogenate. 0.05 mL of the homogenate was taken, added with 0.3 mL of acetonitrile containing the internal standard substance megestrol (100 ng/mL) and 0.005 mL of blank acetonitrile, fully vortexed for 1 minute on a vortex mixer, and centrifuged for 10 minutes (14000 g); about 0.15 mL of the supernatant was taken and placed into an injection vial, injected 5 L to LC-MS/MS for detection and analysis by referring to the method of Test Example 4. In combination with the standard curve obtained in above section 2, the concentrations of Compound (1) in rat brain tissue and spinal cord samples were calculated.

(140) TABLE-US-00008 TABLE 7 Concentrations of Compound (1) in rat plasma, rat brain tissue, rat spinal cord after administration for 2 hours (n = 3). Concentration Rat plasma Rat brain Rat spinal K.sub.p (ng/mL) tissue (ng/g) cord (ng/g) K.sub.p(b/p) K.sub.p(s/p) 270.7 47.4 808.3 233.8 998.3 393.5 3.0 0.71 3.7 1.3

(141) The results as shown in Table 3 indicated that after exposure in plasma, Compound (1) easily entered the brain tissue and the spinal cord through blood-brain barrier, and its contents in the brain tissue and in the spinal cord were 3-4 times of the content in plasma; when the compound was used as an analgesic drug of central target, this distribution characteristic was beneficial to its efficacy.

(142) It is apparent that the above-described examples are merely provided for illustrative purpose, and are not intended to limit embodiments. For those skilled in the art, other variations or modifications in various forms may be made in light of the above description. There is no need and no way to exhaust all of the embodiments. That is, obvious changes or variations resulting therefrom are still within the scope of the invention.

REFERENCE DOCUMENTS

(143) [1] Newton P M, Messing R O. Channels, 2009, 3(2): 77-81. [2] Tomoyuki KONDA, Azusa ENOMOTO, Akira TAKAHARA, and hiroshi YAMAMOTO, Effects of LN-Type Calcium Channel Antagonist, Cilnidipine on Progressive Renal Injuries in Dahl Salt-Sensitive Rats. Biol. Pharm. Bull. 2006, 29(5) :933-937. [3] Tomoyuki Konda, Azusa Enomoto, et al. The N- and L-Type Calcium Channel Blocker Cilnidipine Suppresses Renal Injury in Dahl Rats Fed a high-Sucrose Diet. an Experimental Model of Metabolic Syndrome Nephron Physiol, 2005, 101:1-13. [4] Ishikawa T, Nobukata h, et al. Effects of Ca.sup.2+ channel blocker cilnidipine in comparison with that of amLodipine and nifedipine on insulin resistance in Dahl S rats. Jpn Pharmacol Ther. 1999, 27, 53-60. [5] Yamamoto T, Takahara A. Current Topics in Med Chem., 2009, 9: 377-395. [6] Teodori. J Med Chem, 2004, 47: 6070-6081. [7] Knutsen. Bioorg Med Chem Lett, 2007, 17(3): 662-667. [8] Yamamoto. Bioorg Med Chem, 2006, 14: 5333-5339. [9] Yamamoto. Bioorg Med Chem Lett, 2008, 18(17): 4813-4816. [10] Tyagarajan. Bioorg Med Chem Lett, 2011, 21(17): 869-873. [11] Zamponi G W, et al. Bioorg Med Chem Lett, 2009, 19: 6467-6472. [12] Pajouheshh, et al. Bioorg Med Chem Lett, 2010, 20: 1378-1383. [13] Hou Yunde. Guide to Molecular Cloning Experiments (2.sup.nd Edition), Science Press, 2002. [14] Lai Maode. Medical Molecular Biology, People's Medical Publishing House, 1999. [15] Davis B, Morris T. Pharma Res, 1993, 10, 1093-1095.