A PROCESS PHOTOMETER ARRANGEMENT

Abstract

Disclosed is a process photometer arrangement (10) with a photometric cuvette unit (20) comprising a cuvette body (24) defining a cuvette cavity (25) and a combined sample inlet/outlet opening (34), a photometer light inlet window (60) and a photometer light outlet window (62). The cuvette body (24) is movably supported by a cuvette body support element (88) and the cuvette unit (20) is provided with a cuvette vibration device (80) for vibrating the cuvette body (24) to thereby quickly and homogenously mix a liquid sample volume (31) within the cuvette cavity (25) without any separate mixing element within the cuvette cavity. This allows a short mixing action within a microfluidic cuvette.

Claims

1-15. (canceled)

16. A process photometer arrangement with a photometric cuvette unit comprising a cuvette body defining a cuvette cavity and a sample inlet opening, a sample outlet opening, a photometer light inlet window and a photometer light outlet window, wherein the cuvette body is movably supported by a cuvette body support element and the cuvette unit is provided with a cuvette vibration device for vibrating the cuvette body to thereby homogenously mix a liquid sample volume within the cuvette cavity.

17. The process photometer arrangement according to claim 16, wherein the cuvette vibration device generates a cuvette vibration frequency of 1 to 500 Hz.

18. The process photometer arrangement according to claim 16, wherein the cuvette vibration device generates a linear cuvette vibration, preferably with a nominal vibration amplitude of 0.1 to 5.0 mm.

19. The process photometer arrangement according to claim 16, wherein the cuvette body defines at least one inclined interior side wall being inclined with an inclination angle of between 150 and 750 with respect to a vertical.

20. The process photometer arrangement according to claim 16, wherein the cuvette body defines two opposite parallel vertical interior sidewalls and two opposite inclined interior side walls both being inclined to define a V-shaped funnel between the vertical interior sidewalls, wherein the liquid outlet opening is provided at the bottom of the V-shaped funnel.

21. The process photometer arrangement according to claim 16, wherein the cuvette vibration device is a linear horizontal vibration device and the linear horizontal vibration axis is substantially lying in the V-plane and parallel to the vertical interior sidewalls.

22. The process photometer arrangement according to claim 16, wherein the cuvette cavity is fluidically closed.

23. The process photometer arrangement according to claim 16, wherein the sample inlet opening and the sample outlet opening are defined by one single combined inlet and outlet opening.

24. The process photometer arrangement according to claim 16, wherein the cuvette body is provided with a venting opening for providing an air cushion above the sample volume in the cuvette cavity during the cuvette vibration caused by the vibration device.

25. The process photometer arrangement according to claim 16, wherein the cuvette vibration device is provided with an electromagnet which is driven by a frequency generator.

26. The process photometer arrangement according to claim 16, wherein the cuvette body is provided with a reagent inlet opening for directly applying a liquid reagent into the cuvette cavity.

27. The process photometer arrangement according to claim 16, wherein the cuvette body is provided with a flushing liquid opening.

28. The process photometer arrangement according to claim 16, wherein the cuvette cavity has a total volume of less than 0.5 ml.

29. The process photometer arrangement according to claim 16, wherein the photometer light inlet window and the photometer light outlet window is provided at sidewalls of the cuvette cavity.

30. The process photometer arrangement according to claim 16, comprising a control unit with a sample filling level control unit being connected to a photometer unit for controlling the liquid sample filling level within the cuvette cavity.

Description

[0026] One embodiment of the invention is described with reference to the enclosed drawings, wherein

[0027] FIG. 1 shows schematically a process photometer arrangement with a photometric cuvette unit including a cuvette vibration device in a horizontal cross-section I-I, and

[0028] FIG. 2 shows schematically the process photometer arrangement with the photometric cuvette unit in a vertical cross section II-II.

[0029] The figures show a microfluidic process photometer arrangement 10 for quantitatively determining the concentration of several different analytes of wastewater 12 in a waste water tank 12 of a waste water plant.

[0030] A raw liquid sample of the wastewater 12 is pumped by a sample pump 13 through an immersed sample filter element 11 to the process photometer arrangement 10. After the process photometer arrangement 10 has provided one or more photometric measurement actions, the liquid sample is pumped by a drain pump 15 from the process photometer arrangement 10 to a drain tank 14 where the measured liquid samples volume 14 are accumulated. If the used reagent quantity is not harmful for the environment, the liquid sample alternatively can be pumped back into the wastewater tank 12 after the photometric measurement actions. The process photometer arrangement 10 is a part of a closed-loop circuit to control one or more analyte of the waste water plant. The process photometer arrangement 10 works, in this embodiment, batch-wise.

[0031] The process photometer arrangement 10 is provided with a photometric cuvette unit 20 and with a cuvette vibration device 80. The photometric cuvette unit 20 is substantially defined by a transparent cuvette body 24 defining an interior cuvette cavity 25. The cuvette body 24 is at least partially shielded by a shielding body 22 to optically shield the cuvette cavity 25 against external light.

[0032] The cuvette cavity 25 is defined by two inclined interior sidewalls 26,27 being inclined with an inclination angle a of 45 with respect to a vertical axis and by two vertical interior sidewalls 28, 29 being parallel and opposite to each other. The inclined interior sidewalls 26,27 define a V-shaped funnel between the vertical interior sidewalls 28,29. One single combined liquid inlet/outlet opening 34 is provided at the bottom of the V-shaped funnel. At the top of the cuvette cavity 25, a venting opening 35 is defined by the cuvette body 24 for venting and flushing the cuvette interior cavity 25. The venting opening 35 is fluidically connected via a flexible line section 48 and a flushing pump 42 to a flushing liquid tank 40 comprising a flushing liquid 40 and alternatively via a venting pump 44 to an atmosphere opening 46. The cuvette cavity 25 has a total volume of 50 to 70 l.

[0033] Three reagent inlet openings 61,62,63 are provided at one vertical interior sidewall 29 for applying three different reagents 51,52,53 into the cuvette cavity 25. The liquid reagents 51,52,53 are stored in corresponding reagent tanks 51,52,53 defining a reagent unit 50 and can be pumped separately by corresponding reagent pumps 56,56,56 from the corresponding reagent tank 51,52,53 into the cuvette cavity 25.

[0034] The reagent lines are provided with flexible reagent line sections 58,58,58 for mechanically coupling the corresponding reagent line to the cuvette body 24 which is movably supported by two cuvette body support elements 88 which are elastic sheet spring elements.

[0035] The combined inlet/outlet opening 34 is connected to the pumps 13,15 by a flexible tube section 36.

[0036] The photometer arrangement 10 is provided with a spectrometric photometer unit 18 which is optically coupled by optical fiber elements 66, 66 and flexible fiber sections 64 to a photometer light inlet window 60 and a photometer light outlet window 62 at the vertical interior cuvette sidewalls 28, 29. The photometer light inlet window 60 and the photometer light outlet window 62 are arranged in-line to each other.

[0037] The photometer arrangement 10 is provided with a cuvette vibration device 80 vibrating the cuvette body 24 with a varying vibration frequency between 10 and 200 Hz and with a varying vibration amplitude of 1.0 to 3.0 mm in a horizontal linear vibration axis x. The cuvette vibration device 84 is provided with a static electromagnet 84 which is driven by a frequency generator 86 and with a permanent magnet 82 which is fixed to the cuvette body 24 which is elastically movable in the horizontal linear vibration axis x.

[0038] The photometer arrangement 10 is controlled by a control unit 16 comprising a sample filling level control unit 16 which is electronically connected to the photometer unit 18 for controlling the liquid sample filling level within the cuvette cavity 25. The control unit 16 substantially controls the pumps 13,15,42,44,56,56,56, the photometer unit 18 and the frequency generator 86 of the cuvette vibration device 80.

[0039] The process photometer arrangement 10 works batch-wise, so that a measurement sequence starts with the pump 13 pumping a raw liquid sample from the tank 12 to the cuvette cavity 25. The venting pump 44 pumps air from the cuvette cavity 25 to the atmosphere. The rising level of the raw liquid sample within the cavity 25 is controlled by the sample filling level control unit 16. The sample pumping action is stopped as soon as the liquid level within the cuvette cavity 25 is above the light inlet window 60 and the light outlet window 62 so that an air cushion 32 remains above the liquid volume 31 with in the cuvette cavity 25.

[0040] The control unit 16 activates the respective reagent pump 56 to pump the needed reagent liquid 51 into the cuvette cavity 25. Subsequently, the control unit 16 activates the cuvette vibration device 80 which generates a varying vibration frequency of 10 to 200 Hz with a varying vibration amplitude of 1 to 3 mm for about one minute. The prepared sample volume 30 within the cuvette cavity 25 is thereby continuously mixed. During the mixing action, the photometer 18 is working already and detects the shifting of the measurement value with a particular discrete measurement wavelength. As soon as the shifting of the measurement value stops, the vibration device 80 is stopped and a final absorption measurement is provided, and the final measurement value is stored.

[0041] After that, the liquid volume 30 with the cuvette cavity 25 is pumped out of the cavity 25 by the drain pump 15 until the cuvette cavity 25 is empty. Finally, the cuvette cavity 25 is cleaned by flushing the cuvette cavity 25 with a cleaning/flushing liquid 40 pumped by the corresponding pump 42 into the cuvette 25 and by finally pumping the flushing liquid to the drain tank 14. The cuvette unit 20 is now ready for the following measurement sequence.