SLIDE CLEANER

Abstract

The disclosure describes a slide cleaner apparatus. The apparatus comprises a cleaning chamber configured to receive a cell counting slide, at least one fluid inlet arranged to substantially align with an inlet on a cell counting slide when located in the chamber, and configured to feed cleaning fluid into the cleaning chamber and slide, and at least one fluid outlet configured to remove cleaning fluid from the cleaning chamber and slide. The disclosure also extends to a method of cleaning a cell counting slide.

Claims

1. A slide cleaner apparatus comprising a cleaning chamber configured to receive a cell counting slide, at least one fluid inlet arranged to substantially align with an inlet on a cell counting slide when located in the chamber, and configured to feed cleaning fluid into the cleaning chamber and slide, and at least one fluid outlet configured to remove cleaning fluid from the cleaning chamber and slide.

2. A slide cleaner apparatus according to claim 1, wherein the cleaning chamber defines a maximum length of between 50 mm and 200 mm, a maximum width of between 10 mm and 100 mm and a depth of between 1 mm and 20 mm.

3. A slide cleaner apparatus according to claim 1, wherein the at least one fluid inlet is disposed in the base of the chamber and/or is substantially semi-circular shape and/or comprises a fluid injector configured to feed cleaning fluid into the cleaning chamber and slide, and the injector extends between 0.01 mm and 3 mm above the base of the chamber.

4. (canceled)

5. (canceled)

6. A slide cleaner apparatus according to claim 1, wherein the apparatus comprises two inlets, and each inlet is arranged to substantially align with an inlet on a cell counting slide when located on the chamber.

7. A slide cleaner apparatus according to claim 1, wherein at least one fluid outlet is arranged to substantially align with an outlet on the cell counting slide and/or is disposed in the base of the chamber and/or is a substantially rectangular shape.

8. (canceled)

9. (canceled)

10. A slide cleaner apparatus according claim 1, wherein the apparatus comprises two outlets, and each outlet is arranged to substantially align with an outlet on a cell counting slide when located on the chamber.

11. A slide cleaner apparatus according to claim 1, wherein the base of the chamber comprises a silicon lining, optionally wherein the silicon lining has a Shore A hardness of between 5 and 40 at 20 C.

12. (canceled)

13. A slide cleaner apparatus according to claim 1, wherein the slide cleaner comprises a cover configured to removably cover the cleaning chamber, and a slide when located in the chamber, optionally wherein the cover comprises at least one projection disposed on an underside of the cover, such that when the cover is in the closed configuration the or each projection contacts a slide when located in the chamber.

14. A slide cleaner apparatus according to claim 13, wherein the apparatus comprises locking means configured to lock the cover in the closed configuration, optionally wherein the locking means comprises a stud disposed on the cover and a further stud disposed on a locking point, wherein the studs are disposed substantially adjacent to each other when the cover is in the closed configuration, wherein both of the studs comprise a magnet.

15. (canceled)

16. (canceled)

17. A slide cleaner apparatus according to claim 13, wherein the apparatus comprises a sensor configured to detect if a slide is disposed in the cleaning chamber and the cover is in the closed configuration.

18. A slide cleaner apparatus according to claim 17, wherein the sensor comprises: a projection movable between a first, extended position in which the projection extends above the base of the chamber and a second, retracted position in which the projection lies substantially flush with the base of the chamber; and biasing means configured to bias the sensor into the first, extended position; and the projection is configured to move into the second, retracted position when a slide is placed in the chamber and the cover is moved into the closed configuration, optionally wherein in the first, extended position, the projection extends a height above the base of the chamber which is less than the depth of the chamber.

19. (canceled)

20. A slide cleaner apparatus according to claim 18, wherein the apparatus comprises ejection means configured to eject a slide from the chamber, optionally wherein the ejection means comprises the sensor, and the projection is movable to a third, further extended configuration, in which the projection extends a height above the base of the chamber which is greater than the depth of the chamber.

21. (canceled)

22. A slide cleaner apparatus according to claim 1, wherein the slide cleaner further comprises a cleaning fluid reservoir configured to store cleaning fluid therein, a cleaning fluid conduit extending between the cleaning fluid reservoir and the at least one fluid inlet, and configured to transport cleaning fluid from the cleaning fluid store to the or each inlet, and a cleaning fluid pump configured to pump cleaning fluid from the cleaning fluid reservoir, along the cleaning fluid conduit to the or each inlet and/or wherein the slide cleaner comprises a waste fluid reservoir configured to store waste fluid therein, a waste fluid conduit extending between the or each outlet and the waste fluid reservoir, and configured to transport fluid from the or each outlet to the waste fluid reservoir, and a vacuum pump configured to draw fluid from the or each outlet, along the waste fluid conduit and into the waste fluid reservoir.

23. A slide cleaner apparatus according to claim 1, wherein the cleaning fluid comprises water and between 0.01 and 20 wt % of a surfactant.

24. (canceled)

25. A slide cleaner apparatus according to claim 1, wherein the slide cleaner comprises control means configured to run a wash cycle on the apparatus and activation means configured to send a signal to the control means, and thereby initiate the wash cycle, optionally wherein the apparatus is configured to run an automatic wash cycle which comprises a duration of between 0.001 and 120 seconds.

26. (canceled)

27. A method of cleaning a cell counting slide, the method comprising: placing a cell counting slide into a cleaning chamber; substantially aligning an inlet of the slide with a cleaning fluid inlet in the chamber; feeding cleaning fluid through the cleaning fluid inlet, into the cleaning chamber and the inlet of the slide and allowing the cleaning fluid to flow through the slide; and removing fluid from the cleaning chamber and slide via a cleaning fluid outlet in the chamber.

28. A method according to claim 27, wherein discharging a cleaning fluid into the chamber comprises discharging a cleaning fluid into the chamber for between 1 and 3 seconds and/or removing fluid from the cleaning chamber comprises removing fluid from the cleaning chamber and slide simultaneously to discharging a cleaning fluid into the chamber.

29. (canceled)

30. A method according to claim 27, wherein discharging a cleaning fluid into the chamber comprises discharging a cleaning fluid into the chamber between 1 and 30 times wherein each time is separated by a pre-determined interval, optionally wherein the pre-determined interval is between 0.5 and 3 seconds.

31. (canceled)

32. A method according to claim 30, wherein the method comprises removing fluid from the cleaning chamber during the or each pre-determined interval.

33. A method according to any one of claim 27, wherein after cleaning fluid has been discharged into the chamber the method comprises drying the slide by removing fluid from the cleaning chamber for between 0.5 and 30 seconds, optionally wherein drying the slide comprises feeding air into the inlets for between 0.5 and 30 seconds under a positive pressure.

34. (canceled)

Description

[0070] Embodiments of the present invention will now be described, by way of example only, with reference to the accompanying drawings, in which:

[0071] FIG. 1 is a front view of a slide cleaner according to an embodiment of the present invention;

[0072] FIG. 2 is a side perspective view of the slide cleaner of FIG. 1;

[0073] FIG. 3 shows two embodiments of a cell counting slide which can be cleaned using a slide cleaner, FIG. 3a is a Countess cell counter slide; FIG. 3b is a Bio-Rad TC20 cell counter slide and; FIG. 3c is Luna cell counter slide;

[0074] FIG. 4 is a perspective view of a slide support platform which forms part of the slide cleaner of FIGS. 1 and 2;

[0075] FIG. 5 is a top plan view of the slide support platform of FIG. 4;

[0076] FIG. 6 is a top plan view of an alternative slide support platform;

[0077] FIG. 7 is a schematic diagram of the slide cleaner;

[0078] FIG. 8 is a schematic diagram illustrating the timings of a suitable wash cycle; and

[0079] FIG. 9 is a front view of an alternative slide cleaner.

[0080] A slide cleaner apparatus 2 is shown in FIGS. 1 and 2, and is used to clean (i.e. wash and dry) a cell counting slide 100, three embodiments of which are shown in FIG. 3. It will be appreciated that the shape of the slides vary. The apparatus 2 shown in FIG. 1 is designed for use with a Countess cell counter slide 100a, as shown in FIG. 3a. However, as explained below, the apparatus can be modified to be used with other slides, such as a Bio-Rad TC20 cell counter slide 100b or a Luna cell counter slide 100c, as shown in FIGS. 3b and 3c. The apparatus 2 comprises a main body 4 which is substantially cuboid in shape. Disposed on a top face 6 of the body 4 is a substantially cuboid platform 8, which is shown in detail in FIG. 4. The platform 8 comprises a first flange 10 which extends from a front face 12 of the platform 8, and second flange (not shown) which extends from a back face (not shown) of the platform 8. Screws 14 extend through the flanges 10 and into the body 4, thereby fixing the platform 8 to the body 4.

[0081] As shown in FIG. 2, the platform 8 defines a chamber 16, which comprises a generally cuboid recess in a top face 18 of the platform 8. The chamber 16 is sized to receive a cell counting slide 100, for instance a Countess cell counting chamber slide similar to the slide 100a shown in FIG. 3a. Such slides 100 are 25 mm75 mm1.65 mm, and the chamber 16 is 26 mm76 mm1.8 mm. The base of the chamber 16 is lined with silicon with a Shore A hardness of 27 at 20 C. for improving the seal with a slide 100 when inserted therein.

[0082] As shown in FIG. 4, two spaced apart outlets 26 defining rectangular slits with a length of 1.5 mm and a width of 6 mm are defined in the base of the chamber 16. The outlets 26 are disposed centrally between the left and right 23, 25 sides of the chamber, i.e. with a gap of 10 mm between the outlets 26 and either side 23, 25 of the chamber. The first outlet 26 is disposed 34 mm from a front side 22 of the chamber, the second outlet 26 is disposed 34 mm from a rear side 24 of the chamber, and the gap between the first and second outlets 26 is 4 mm.

[0083] A first inlet 27 is disposed between the outlets 26 and a front side 22 of the chamber 16, and a second inlet 27 is disposed between the outlets 26 and a back side 24 of the chamber 16. Both the inlets 27 define a semi-circular shape with a width of 6 mm and a length of 3 mm. The inlets 27 are disposed centrally between the left and right 23, 25 sides of the chamber, i.e. with a gap of 10 mm between the inlets 27 and either side 23, 25 of the chamber. The first inlet 27 is disposed 18 mm from the front side 22 of the chamber and 13 mm from the first outlet 26. The second inlet 27 is disposed 18 mm from the rear side 24 of the chamber and 13 mm from the second outlet 26. The inlets 27 comprise injectors 31 (see FIG. 5) configured to inject a cleaning liquid into the chamber 16. The injectors 31 extend about 0.5 mm above the base of the chamber 16. The cleaning liquid can comprise 1 wt % washing detergent in water and is stored in a cleaning liquid bottle 28 which is disposed in a bottle holder 29 on a platform 30 to the rear of the body 4, as shown in FIG. 1. When a pump 66 is activated it causes the cleaning liquid to flow along a tube 32 which extends between the bottle 28 and the injectors 31 for each inlet 27. This configuration is shown schematically in FIG. 7.

[0084] The outlets 26 comprise vacuum points configured to remove waste or spent washing fluid from the chamber 16. Waste washing fluid removed from the chamber 16 is transported by a tube 34 to a waste bottle 36 which is also disposed in a bottle holder 38 on the platform 30. A further tube 40 extends from the waste bottle 36, and when a vacuum pump 68 disposed on the further tube 40 is activated it causes gas to flow from the waste bottle 36, through the further tube 40 and into the atmosphere, thereby creating a negative pressure in the waste bottle 36.

[0085] A hinge 42 is fixed by screws 44 to one side 46 of the platform 8, and a cover 48 made from a clear perspex acrylic sheet is attached to the hinge 42. FIG. 1 shows the cover 48 disposed over the chamber 16 in a closed configuration, and FIG. 2 shows the cover 48 in an open configuration, allowing access to the chamber 16. Three projections 50 are disposed on the underside 52 of the cover 48. In the closed configuration, the projections 50 are disposed directly above the chamber 16, such that the central projection aligns with the outlets 26 and the two outer projections align with the inlets 27.

[0086] A neodymium magnet 54 is also disposed on the underside of the cover 48, and a column 56 extends upwards from the top face 6 of the body 4 to one right of the platform 8. A further neodymium magnet 58 is mounted at the top of the column 56. In the closed configuration, the magnet 54 on the cover 48 is disposed directly above the magnet 58.

[0087] Extending upwards from the base of the chamber 16, there is provided a moveable sensor 20 configured to sense if a slide 100 is disposed in the chamber 16 when the cover 48 is in the closed configuration. When no slide 100 is inserted in the chamber 16, the sensor 20 is disposed in a first, extended position, whereby the sensor extends about 1 mm upwardly from the base of the chamber 16. When a slide 100 is inserted into the chamber 16 and the cover 48 is moved to the closed configuration, then the projections 50 will push down on the slide 100 which in turn will push down on the sensor 20 and cause it to move into a second, retracted position where the top of the sensor 20 is level with the base of the chamber 16. Accordingly, this allows the apparatus 2 to sense when a slide 100 is disposed in the chamber 16 and the cover 48 is in the closed configuration. Due to a spring (not shown) biasing the sensor into the first, extended position, the weight of the slide 100 is insufficient to cause the sensor 100 to move to the second, retracted position if the cover 48 is open. Similarly, if the slide 100 is not present, then the sensor 20 will remain in the first, extended position even when the cover 48 is in the closed configuration.

[0088] As explained in more detail below, the sensor is also configured to move to a third, further extended position whereby the sensor extends about 7 mm upwardly from the base of the chamber 16. This aids a user in removing a slide 100 from the chamber 16.

[0089] In use, a user inserts a slide 100 into the chamber 16 and then moves the cover 48 to the closed configuration, as shown in FIG. 1. When in position, the slide 100 is located such that inlets 104 in slide 100 align with wash liquid inlets 27, and outlets 102 in the slide 100 align with wash liquid outlets 26. The user then presses a start button 64 disposed in the body 4, which causes a controller disposed in the body 4 to initiate a wash cycle. First, the controller checks the position of the sensor 20, and when it finds that is in the second, retracted position, it will start a wash cycle. The controller will only start a wash cycle if the sensor is in the second, retracted position.

[0090] Upon starting the wash cycle, the controller activates the pumps 66, 68. The pump 66 feeds the cleaning liquid along pipe 32, out of the outlets 26 and into the chamber 16. The vacuum pump 68 feeds air from the waste bottle 36 through pipe 40 creating a negative pressure in the waste bottle 36. This causes air to flow through air inlets 70, chamber inlets 27 and slide inlets 104 into the cell chambers 106. The negative pressure also causes the air and cleaning fluid to continue to flow through the cell chambers 106, through the cell outlets 102 and chamber outlets 26, along pipe 34 and into the waste bottle 36. The stream of fluid dislodges cells and any waste liquid from the cell chamber 106. Furthermore, the negative pressure created inside the cell chambers 106 due to air being sucked out of the outlets 26, and a seal being created due to the slide 100 being pressed onto the silicon-lined base of the chamber 16, by projections 50 on the cover 48 when in a closed position and firmly holds the slide 100 in place.

[0091] After one second, the controller turns off the pump 66, but maintains the vacuum pump 68 in the on configuration.

[0092] This cycle of flushing the chambers 106 with the combination of air flow (vacuum) and cleaning fluid is repeated four more times, as shown in FIG. 8. The inventor has found that this is sufficient to clean the slide loft After the final cycle, the vacuum pump 68 is maintained in the on configuration, thereby flushing the chambers 106 with air to dry the slide 100, for a final 20 or 30 seconds. The controller then turns off the vacuum pump 68, thereby completing the wash cycle.

[0093] Once the wash cycle has been completed, a user may move the cover 48 to the open configuration, thereby causing the sensor 20 to move to the first, extended position due to the biasing force of the spring. A user may then press the start button 64 again. As before, this will cause the controller to check the position of the sensor 20, and when it finds that is in the first, extended position, it will cause the sensor to move to the third, further extended position thereby lifting one side of the slide 100 and aiding its removal from the chamber 16. The cleaned slide 100 may then be reused. The sensor 20 will remain in the third, further extended position while the user maintains pressure on the button 64, and once the user releases the button 64 the sensor will revert to the first, extended position.

[0094] Buttons 60 and 62 allow a user to control the apparatus manually. Accordingly, button 62 activates and deactivates both the vacuum pump 68 and cleaning fluid pump 66, for flushing the cell chamber 106 of the slide 100. Button 60 activates and deactivates only the vacuum pump 68, causing airflow in the cell chamber 106 of the slide 100, for drying purposes.

Alternative Embodiments

[0095] As mentioned above, the chamber 16 shown in FIGS. 4 and 5 and described above is configured to receive a Countess cell counter slide 100a, as shown in FIG. 3a. However, alternative slides have alternative dimensions and will require alightly different sized chambers. Accordingly, FIG. 6 shows a chamber 16 which is configured to receive a Bio-Rad TC20 cell counter slide 100b, as shown in FIG. 3b.

[0096] The chamber 16 shown in FIG. 6 has an irregular shape corresponding to the shape of the Bio-Rad TC20 cell counter slide 100b. The chamber has a maximum width of 26 mm, a maximum length of 76 mm and a depth of 2 mm.

[0097] As with the previously described chamber, the base of the chamber 16 shown in FIG. 6 is also lined with silicon with a Shore A hardness of 27 at 20 C., and a moveable sensor 20 is provided.

[0098] Two spaced apart outlets 26 defining rectangular slits with a length of 1.5 mm and a width of 6 mm are defined in the base of the chamber 16. The first outlet 26 is disposed 11.5 mm from the left side 23 of the chamber 16 and 8.5 mm from the right side 25 of the chamber 16. Conversely, the second outlet 26 is disposed 8.5 mm from the left side 23 of the chamber 16 and 11.5 mm from the right side 25 of the chamber 16. The first outlet 26 is disposed 34 mm from a front side 22 of the chamber 16, the second outlet 26 is disposed 34 mm from a rear side 24 of the chamber 16, and the gap between the first and second outlets 26 is 5 mm.

[0099] A first inlet 27 is disposed between the outlets 26 and a front side 22 of the chamber 16, and a second inlet 27 is disposed between the outlets 26 and a back side 24 of the chamber 16. Both the inlets 27 define a semi-circular shape with a width of 6 mm and a length of 3 mm. Similarly to the outlets 26, the first inlet 27 is disposed 11.5 mm from the left side 23 of the chamber 16 and 8.5 mm from the right side 25 of the chamber 16 and the second inlet 26 is disposed 8.5 mm from the left side 23 of the chamber 16 and 11.5 mm from the right side 25 of the chamber 16. The first inlet 27 is disposed 19 mm from the front side 22 of the chamber and 14 mm from the first outlet 26. The second inlet 27 is disposed 19 mm from the rear side 24 of the chamber and 14 mm from the second outlet 26. As with the previously described chamber, the inlets 27 comprise injectors 31 configured to inject a cleaning liquid into the chamber 16.

[0100] While, not shown it will be appreciated that alternative chambers 16 could be provided for use with other slides, such as the Luna cell counter slide 100c.

[0101] It will be appreciated that in a further alternative embodiment, in addition to or instead of the vacuum pump 68, the apparatus may comprise a further pump configured to pump air into inlets 27. This pump could be activated for the whole of the wash cycle, or only for the drying phase.

Further Alternative Embodiment

[0102] A further alternative embodiment is shown in FIG. 9. The embodiment comprises a slide cleaner apparatus 2. Similar to the embodiment shown in FIG. 1, the apparatus 2 shown in FIG. 9 comprises a main body 4 which is substantially cuboid in shape. Disposed on a top face 6 of the body 4 is a substantially cuboid platform 8, comprising a first flange 10 which extends from a front face 12 of the platform 8, and second flange (not shown) which extends from a back face (not shown) of the platform 8. Screws 14 extend through the flanges 10 and into the body 4, thereby fixing the platform 8 to the body 4.

[0103] The platform 8 defines a chamber 16, which is similar to the chamber described above in relation to the embodiment shown in FIGS. 1 and 2. However, unlike the previously described embodiment, the base of the chamber 16 is lined with silicon with a Shore A hardness of 15 at 20 C. for improving the seal with a slide 100 when inserted therein.

[0104] As explained in more detail above, the chamber comprises outlets 26 which comprise vacuum points configured to remove waste or spent washing fluid from the chamber 16. Waste washing fluid removed from the chamber 16 is transported by a tube 34 to a waste bottle 36 which is also disposed in a bottle holder 38 on a platform 30. A further tube 40 extends from the waste bottle 36, and when a vacuum pump 68 disposed on the further tube 40 is activated it causes air to flow from the waste bottle 36, through the further tube 40 and into the atmosphere, thereby creating a negative pressure in the waste bottle 36.

[0105] In the illustrated embodiment, the further tube 40 comprises a hydrophobic filter 200. The filter 200 is a hydrophobic PTFE (polytetrafluoroethylene) filter with a pore size of 0.22 m. This filter 200 prevents any waste over flow from waste bottle 36 from entering into the vacuum pump 68.

[0106] As described in relation to the embodiment illustrated in FIGS. 1 and 2, a hinge 42 attaches a cover 48 to the platform 8. In the illustrated embodiment, two projections 50 are disposed on the underside 52 of the cover 48. In the closed configuration, the projections 50 are disposed directly above the inlets 27.

[0107] A neodymium magnet 54 is also disposed on the underside of the cover 48. Furthermore, in the embodiment illustrated in FIG. 9, a further neodymium magnet 202 is disposed in the platform 8. In the closed configuration, the magnet 54 on the cover 48 is disposed directly above the magnet 202 disposed in the platform 8.

[0108] The platform further comprises a bumper 204. The bumper 204 is the tip of a spring loaded slow motion piston, the outer layer of which comprises rubber. The bumper 204 prevents the cover 48 from slamming when closed.

[0109] In use, a user inserts a slide 100 into the chamber 16 and then moves the cover 48 to the closed configuration. When in position, the slide 100 is located such that inlets 104 in slide 100 align with wash liquid inlets 27, and outlets 102 in the slide 100 align with wash liquid outlets 26. As explained in more detail below, the apparatus 2 comprises a toggle switch 206 which is biased in a central position.

[0110] If the user leaves the toggle switch 206 in the central position and press a start button 208 disposed in the body 4 thereby causing the apparatus to initiate a wash cycle. First, the controller checks the position of the sensor 20, and when it finds that is in the second, retracted position, it will start a wash cycle. The controller will only start a wash cycle if the sensor is in the second, retracted position.

[0111] Upon starting the wash cycle, the controller activates the pumps 66, 68. The pump 66 feeds the cleaning liquid along pipe 32, out of the outlets 26 and into the chamber 16.

[0112] The vacuum pump 68 feeds air from the waste bottle 36 through pipe 40 creating a negative pressure in the waste bottle 36. This causes air to flow through air inlets 70, chamber inlets 27 and slide inlets 104 into the cell chambers 106. The negative pressure also causes the air and cleaning fluid to continue to flow through the cell chambers 106, through the cell outlets 102 and chamber outlets 26, along pipe 34 and into the waste bottle 36. The stream of fluid dislodges cells and any waste liquid from the cell chamber 106. Furthermore, the negative pressure created inside the cell chambers 106 due to air being sucked out of the outlets 26, and a seal being created due to the slide 100 being pressed onto the silicon-lined base of the chamber 16, by projections 50 on the cover 48 when in a closed position and firmly holds the slide 100 in place.

[0113] After one second, the controller turns off the pump 66, but maintains the vacuum pump 68 in the on configuration.

[0114] This cycle of flushing the chambers 106 with the combination of air flow (vacuum) and cleaning fluid is repeated four more times, as shown in FIG. 8. The inventor has found that this is sufficient to clean the slide 100. After the final cycle, the vacuum pump 68 is maintained in the on configuration, thereby flushing the chambers 106 with air to dry the slide 100, for a final 20 or 30 seconds. The controller then turns off the vacuum pump 68, thereby completing the wash cycle.

[0115] If the user does not want to run an automated wash cycle as described above, they can run a manual wash or dry cycle by manipulating a toggle switch 206. If the user pushes the toggle switch 206 to the right this will cause the apparatus 2 to run a manual wash cycle. First the controller checks the position of the sensor 20, and if it finds that is in the second, retracted position, it will activates both of the pumps 66, 68. The controller will only activate the pumps 66, 68 if the sensor is in the second, retracted position. The pumps 66, 68 will continue to run, causing cleaning fluid and air to flow through the chamber 16, until the user releases the toggle switch 206. Once the toggle switch is released the controller will turn off both of the pumps 66, 68, and the toggle switch will return to the central position.

[0116] Alternatively, the user can push the toggle switch 206 to the left to cause the apparatus 2 to run a manual drying cycle. First the controller checks the position of the sensor 20, and if it finds that is in the second, retracted position, activates the vacuum pump 68.

[0117] The controller will only activate the vacuum pump 68 if the sensor is in the second, retracted position. The vacuum pump 68 will continue to run, causing air to flow through the chamber 16, until the user releases the toggle switch 206. Once the toggle switch is released the controller will turn off the vacuum pump 68, and the toggle switch will return to the central position.

[0118] Once the manual or autmoatic cycle has been completed, a user may move the cover 48 to the open configuration, thereby causing the sensor 20 to move to the first, extended position due to the biasing force of the spring. A user may then press the start button 208 again. As before, this will cause the controller to check the position of the sensor 20, and when it finds that is in the first, extended position, it will cause the sensor to move to the third, further extended position thereby lifting one side of the slide 100 and aiding its removal from the chamber 16. The slide 100 may then be reused. The sensor 20 will remain in the third, further extended position while the user maintains pressure on the button 208, and once the user releases the button 208 the sensor will revert to the first, extended position.

CONCLUSION

[0119] The inventor has found that it is possible to re-use supposedly disposable slides 100 at least fifteen times by cleaning them in the manner described above. This dramatically reduces the costs of using a cell counting machine. It also dramatically reduces the plastic biological wastage helping research laboratories become more ecological and environmentally friendly.

[0120] Furthermore, since the slides are not cleaned manually, the risk of a user being exposed to biologically hazardous material is dramatically reduced.