MICROBIAL COMPOSITION AND MICROORGANISM-BASED METHOD OF MANUFACTURING AND TREATING OF ANIMAL FARMS USING OF THE SAME
20230046446 · 2023-02-16
Inventors
Cpc classification
C12R2001/125
CHEMISTRY; METALLURGY
International classification
Abstract
A microbial composition for improving the conditions in animal farms comprising: a slow-releasing carrier base having a first predetermined percentage by weight (w/w %); and a mixture of microorganisms identified in a 16S rRNA gene sequence so that the composition is capable of denitrification, feces decomposition, and hydrogen sulfide (H.sub.2S) oxidation.
Claims
1. A microbial composition, comprising: a slow-releasing carrier base having a first predetermined percentage by weight (w/w %); and a mixture of microorganisms identified in a 16S rRNA gene sequence so that said microbial composition is capable of denitrification, feces decomposition, and hydrogen sulfide (H.sub.2S) oxidation.
2. The microbial composition of claim 1 wherein said mixture of microorganisms further comprises: Bacillus spp., Paenibaccilus spp., and Lactobacillus spp.
3. The microbial composition of claim 2 wherein said Bacillus spp. further comprise: Bacillus favisporus, Bacillus stratosphericus, Bacillus aryabhattai, Baccillus subtilis, Bacillus mensentericus, Bacillus pumilus, Bacillus xiamenensis, Bacillus megaterium, Bacillus altitudinis, Bacillus velezensis, Bacillus amyloliquefaciens, Bacillus coagulans, Bacillus thuringiensis, Bacillus firmus, Baccillus subtilis, Bacillus mensentericus, Bacillus pumilus, Bacillus coagulans, Bacillus thuringiensis, Bacillus licheniformis and Bacillus firmus; wherein a count of each bacterial species is 10.sup.10-10.sup.13 colony-forming unit (CFU) per kilogram (CFU/kg).
4. The microbial composition of claim 2 wherein said Lactobacillus spp. further comprise: Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus caseii, Lactobacillus fermentum, Lactococcus lactis, Lactobacillus rhamnosus, Lactobacillus reuteri, Lactobacillus paracasei, Lactobacillus helviticus, and Lactobacillus grasseri; wherein a count of each bacterial species is 10.sup.10-10.sup.13 colony-forming unit (CFU) per kilogram (CFU/kg).
5. The microbial composition of claim 2 wherein said Paenibacillus spp. further comprise: Paenibacillus glycanilyticus, Paenibacillus pectinilyticus, Paenibacillus polymyxa, Paenibacillus xylanexedens, Paenibacillus xylanexedens, Paenibacillus amylolyticus, Paenibacillus glucanolyticus, Paenibacillus aceti, Paenibacillus antibioticophila, Paenibacillus cellulosilyticus, Paenibacillus humicus, and Paenibacillus xanthanilyticus; wherein a count of each bacterial species is 10.sup.10-10.sup.13 colony-forming unit (CFU) per kilogram (CFU/kg).
6. The microbial composition of claim 2 further comprising Enterococcus spp.
7. The microbial composition of claim 6 wherein said Enterococcus spp. further comprise Enterococcus faecium and Enterococcus faecalis; wherein a count of each bacterial species is 10.sup.10-10.sup.13 colony-forming unit (CFU) per kilogram (CFU/kg).
8. The microbial composition of claim 2 further comprising Rhodococcus spp. Rhodobacter spp., Nitrosomonas spp., Nitrobacter spp., Paracoccus spp.
9. The microbial composition of claim 7 further comprising Paracoccus denitrificans, Pichia anomala, Saccharomyces cerevisiae, Rhodotorula glutinis, Pediococcus acidilactici, Streptococcus thermophilus, and Fictibacillus phophorivorans; wherein a count of each bacterial species is 10.sup.10-10.sup.13 colony-forming unit (CFU) per kilogram (CFU/kg).
10. The microbial composition of claim 9 further comprising: Aspergiullus oryzae, Aspergillus nigrer, Streptomyces spp., Paecillomyces spp., Metarhizium spp., and Beauveria sp; wherein a count of each fungi species is 10.sup.10-10.sup.13 colony-forming unit (CFU) per kilogram (CFU/kg).
11. A microorganism-based method for improving living conditions in animal farms, comprising: (a) determining whether a target animal farm is an aquatic farm; (b) if said target animal farm is not said aquatic farm and is a swiftlet bird house, identifying beneficial microorganism from samples of said swiftlet bird house; (c) identifying beneficial microorganisms capable of eliminating said pathogenic agents of said swiftlet bird house using a 16S rRNA sequence; (d) synthesizing said beneficial microorganisms for said swiftlet bird house; and (e) encapsulating said antagonistic microorganisms of said swiftlet bird house with a slow releasing carrier to obtain a first composition.
12. The microorganism-based method of claim 11, wherein in said step (a) if said target animal farm house is a dry-land animal farm then repeating said steps (b) to (e) to obtain a second composition.
13. The microorganism-based method of claim 11 further comprising: (e) if said target animal farm is said aquatic farm, repeating steps (b) to (e) to obtain a third composition; and (f) mixing said third composition to a daily food portion; and (g) feeding said aquatic farms with a mixture of said third composition and said daily food portion; wherein said slow releasing carrier is a zeolite.
14. The microorganism-based method of claim 11 wherein said first composition further comprises: a slow-releasing carrier having a first predetermined CFU/kg; and a mixture of microorganisms identified in a 16S rRNA gene sequence so that said composition is capable of denitrification, feces decomposition, and hydrogen sulfide (H.sub.2S) oxidation.
15. The microorganism-based method of claim 14 wherein said mixture of microorganisms further comprises: Bacillus spp further comprising: Bacillus favisporus, Bacillus stratosphericus, Bacillus aryabhattai, Baccillus subtilis, Bacillus mensentericus, Bacillus pumilus, Bacillus xiamenensis, Bacillus megaterium, Bacillus altitudinis, Bacillus velezensis, Bacillus amyloliquefaciens, Bacillus coagulans, Bacillus thuringiensis, Bacillus firmus, Baccillus subtilis, Bacillus mensentericus, Bacillus pumilus, Bacillus coagulans, Bacillus thuringiensis, and Bacillus firmus whose CFU/kg for each species is 10.sup.10-10.sup.13; Lactobacillus spp further comprising: Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus caseii, Lactobacillus fermentum, Lactococcus lactis, Lactobacillus rhamnosus, Lactobacillus reuteri, Lactobacillus paracasei, Lactobacillus helviticus, and Lactobacillus grasseri whose CFU/kg for each species is 10.sup.10-10.sup.13; and Paenibacillus spp. further comprising Paenibacillus glycanilyticus, Paenibacillus pectinilyticus, Paenibacillus polymyxa, Paenibacillus xylanexedens, Paenibacillus xylanexedens, Paenibacillus amylolyticus, Paenibacillus glucanolyticus, Paenibacillus aceti, Paenibacillus antibioticophila, Paenibacillus cellulosilyticus, Paenibacillus humicus, and Paenibacillus xanthanilyticus whose CFU/kg for each species is 10.sup.10-10.sup.13.
16. The microorganism-based method of claim 15 wherein said first composition further comprises: Enterococcus faecium and Enterococcus faecalis whose count for each species is CFU/kg is 10.sup.10-10.sup.13; Rhodococcus spp., Rhodobacter spp., Nitrosomonas spp., Nitrobacter spp., Paracoccus spp.; and Paracoccus denitrificans, Pichia anomala, Saccharomyces cerevisiae, Rhodotorula glutinis, Pediococcus acidilactici, Streptococcus thermophilus, and Fictibacillus phophorivorans whose count for each species is CFU/kg is 10.sup.10-10.sup.13.
17. The microorganism-based method of claim 16 wherein said second composition further comprises: a slow-releasing carrier base having a first predetermined CFU/kg; and a mixture of bacteria identified in a 16S rRNA gene sequence so that said composition is capable of denitrification, feces decomposition, and hydrogen sulfide (H.sub.2S) oxidation.
18. The microorganism-based method of claim 17 wherein said mixture of microorganisms further comprises: Bacillus spp. further comprising: Bacillus favisporus, Bacillus stratosphericus, Bacillus aryabhattai, Baccillus subtilis, Bacillus mensentericus, Bacillus pumilus, Bacillus xiamenensis, Bacillus megaterium, Bacillus altitudinis, Bacillus velezensis, Bacillus amyloliquefaciens, Bacillus coagulans, Bacillus thuringiensis, Bacillus firmus, Baccillus subtilis, Bacillus mensentericus, Bacillus pumilus, Bacillus coagulans, Bacillus thuringiensis, and Bacillus firmus whose CFU/kg for each species is 10.sup.10-10.sup.13; Lactobacillus spp. further comprising: Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus caseii, Lactobacillus fermentum, Lactococcus lactis, Lactobacillus rhamnosus, Lactobacillus reuteri, Lactobacillus paracasei, Lactobacillus helviticus, and Lactobacillus grasseri whose CFU/kg for each species is 10.sup.10-10.sup.13; and Paenibacillus spp. further comprising Paenibacillus glycanilyticus, Paenibacillus pectinilyticus, Paenibacillus polymyxa, Paenibacillus xylanexedens, Paenibacillus xylanexedens, Paenibacillus amylolyticus, Paenibacillus glucanolyticus, Paenibacillus aceti, Paenibacillus antibioticophila, Paenibacillus cellulosilyticus, Paenibacillus humicus, and Paenibacillus xanthanilyticus whose CFU/kg for each species is 10.sup.10-10.sup.13.
19. The microorganism-based method of claim 18 wherein said second composition further comprises Enterococcus faecium and Enterococcus faecalis whose count for each species is CFU/kg is 10.sup.10-10.sup.13; Rhodococcus spp., Rhodobacter spp., Nitrosomonas spp., Nitrobacter spp., Paracoccus spp.; and Paracoccus denitrificans, Pichia anomala, Saccharomyces cerevisiae, Rhodotorula glutinis, Pediococcus acidilactici, Streptococcus thermophilus and Fictibacillus phophorivorans whose count for each species is CFU/kg is 10.sup.10-10.sup.13.
20. The microorganism-based method of claim 19 wherein said second composition further comprises: Aspergiullus oryzae, Aspergillus nigrer, Streptomyces spp., Paecillomyces spp., Metarhizium spp., and Beauveria spp whose count for each species is CFU/kg is 10.sup.10-10.sup.13.
Description
BRIEF DESCRIPTION OF THE DRAWINGS
[0039] The accompanying drawings, which are incorporated in and form a part of this specification, illustrate embodiments of the invention and, together with the description, serve to explain the principles of the invention.
[0040]
[0041]
[0042]
[0043]
[0044]
DETAILED DESCRIPTION OF THE INVENTION
[0045] Reference will now be made in detail to the preferred embodiments of the invention, examples of which are illustrated in the accompanying drawings. While the invention will be described in conjunction with the preferred embodiments, it will be understood that they are not intended to limit the invention to these embodiments. On the contrary, the invention is intended to cover alternatives, modifications and equivalents, which may be included within the spirit and scope of the invention as defined by the appended claims. Furthermore, in the following detailed description of the present invention, numerous specific details are set forth in order to provide a thorough understanding of the present invention. However, it will be obvious to one of ordinary skill in the art that the present invention may be practiced without these specific details. In other instances, well-known methods, procedures, components, and circuits have not been described in detail so as not to unnecessarily obscure aspects of the present invention.
[0046] One embodiment of the invention is now described with reference to
[0047] Next at step 102, all pathogenic microorganisms that cause problems in the selected animal farms are identified. In practice, step 102 is realized by taking samples from existing animal farms to analyze for pathogenic causes. Factors analyzed in step 102 include but not limited to sound, temperature, wind, humidity, smell, lighting, feces, and nest materials. In some aspects of the present invention, step 102 is also performed by sensors such as Internet of Things (IoT). For example, excess amounts of hydrogen sulfide (H.sub.2S), ammonia (NH.sub.3), Carbon monoxide (CO), or Carbon dioxide (CO.sub.2), etc. can be sensed by a wide variety of sensing technologies for gas detection including electrochemical gas sensors, semiconductor gas sensors, moisture absorbing material sensors, and infrared sensors.
[0048] At step 103, after the problems have been thoroughly understood, all beneficial organisms including antagonistic microorganism and agents that can solve the problems in step 102 and ameliorate the total living conditions of animal farms are identified and synthesized. In many aspects of the present invention, the two main problems that adversely affect the swiftlet bird houses are hydrogen sulfide (H.sub.2S) and ammonia (NH.sub.3). These two problems are found in more 95% of the swiftlet bird houses. This is because of the bird drops or guano and the lack of sunlight thereof similar to that of dark caves or the sewages. The synthesizing process of step 102 is described in more details in the Experiment section.
TABLE-US-00001 TABLE 1 Some Examples of Beneficial Microorganisms That Improve the Living Conditions of the Swiftlet Bird Houses No. Microorganism Description 1 Paenibacillus Isolated from catfish pond sediment. Identified by favisporus the 16S rRNA sequence. Used in nitrogen cycle to clean up ammonia (NH.sub.3). 2 Bacillus Isolated from catfish pond sediment. Identified by stratosphericus the 16S rRNA sequence. Used to hydrolysis starch, biodegradable cellulose. 3 Paenibacilus Isolated from catfish pond sediment. Identified by typhea the 16S rRNA sequence. 4 Bacillus Isolated from catfish pond sediment. Identified by aryabhattai: the 16S rRNA sequence.Used to hydrolysis starch, biodegradable cellulose. 5 Fictibacillus Isolated from catfish pond sediment, Identified by phosphorivorans the 16S rRNA sequence. Used for phosho soluble. 6 Bacillus Isolated from catfish pond sediment. Used to xiamenensis hydrolysis protein. Used to generate healthy environment for swiftlets. 7 Bacillus Isolatede from shrimp pond sediment. Identified megaterium by the 16S rRNA sequence. Used for phosho soluble. 8 Bacillus Isolated from agriculture soil. Identified by the 16S altitudinis rRNA sequence. Used to clean up excess metal ions. Used to hydrolysis starch. 9 Bacillus Isolated from catfish pond sediment. Identified by velezensis the 16S rRNA sequence. Used to hydrolysis starch, protein; promoting healthy biosphere for swiftlets, facilitating pairing up and nesting 10 Bacillus Isolated from rhizosphere soil of legum. Identified amylolique- by the 16S rRNA sequence. Used to hydrolysis faciens starch, biosynthesis biofloculant. 11 Baccillus Isolated from many sources. Biosynthesis enzyme subtilis amylase, protease, lipase. Protease, bacteriocine 12 Baccillus Isolated from sediment shrimp ponds. Biosyntheis licheniformis protease, lipase to degradation organic material promoting healthy biosphere for swiftlets, facilitating pairing up and nesting 13 Bacillus Isolated from sediment shrimp ponds. Biosyntheis mensentericus amylase breakdown starch lipase to degradation organic material promoting healthy biosphere for swiftlets, facilitating pairing up and nesting 14 Bacillus Isolated from sediment shrimp ponds. pumilus Biosynthesis enzyme amylase, biofluculant. Rapid degradation of organic material. 15 Bacillus Isolated from food lactic fermentation. Lactic coagulans fermentation and biosynthesis bacteriocine 16 Bacillus Isolated from soil. Biosynthesis endotoxin to kill thuringiensis pests. 17 Bacillus firmus Isolated from soil. Biosynthesis protease 18 Bacillus Isolated from soil. Biosynthesis enzyme cellulase, aryabhattai tao biofluculant 19 Lactobacillus Isolated from food fermentation. Identification by acidophillus Biochemical tests. Biosynthesis bacteriocine and lactic fermentation for acid lactic against pathogen bacteria and mold. 20 Lactobacillus Isolated from pickle. Identification by Biochemical plantarum tests. Biosynthesis bacteriocine and lactic fermentation for acid lactic against pathogen bacteria and mold 21 Lactobacillus Isolated from natural fermentation milk. caseii Identification by Biochemical tests. Biosynthesis bacteriocine and Biosynthesis bacteriocine and lactic fermentation for acid lactic against pathogen bacteria and mold 22 Lactobacillus Isolated from food fermentation lactic. fermentum Identification by Biochemical tests. Biosynthesis bacteriocine and lactic fermentation for acid lactic against pathogen bacteria and mold 23 Streptococcus Isolated from food fermentation lactic. thermophilus Identification by Biochemical tests. Biosynthesis bacteriocine and lactic fermentation for acid lactic against pathogen bacteria and mold 24 Pediococcus Isolated from food fermentation lactic. acidilactici Identification by Biochemical tests. Biosynthesis bacteriocine and lactic fermentation for acid lactic against pathogen bacteria and mold 25 Lactococcus Isolated from food fermentation lactic. lactis Identification by Biochemical tests. Biosynthesis bacteriocine and lactic fermentation for acid lactic against pathogen bacteria and mold 26 Lactobacillus Isolated from food fermentation lactic. rhamnosus Identification by Biochemical tests. Biosynthesis bacteriocine and lactic fermentation for acid lactic against pathogen bacteria and mold 27 Lactobacillus Isolated from food fermentation lactic. reuteri Identification by Biochemical tests. Biosynthesis bacteriocine and lactic fermentation for acid lactic against pathogen bacteria and mold 28 Lactobacillus Isolated from food fermentation lactic. paracasei Identification by Biochemical tests. Biosynthesis bacteriocine and lactic fermentation for acid lactic against pathogen bacteria and mold 29 Lactobacillus Isolated from food fermentation lactic. helviticus Identification by Biochemical tests. Biosynthesis bacteriocine and lactic fermentation for acid lactic against pathogen bacteria and mold 30 Lactobacillus Isolated from food fermentation lactic. grasseri Identification by Biochemical tests. Biosynthesis bacteriocine and lactic fermentation for acid lactic against pathogen bacteria and mold 31 Enterococcus Isolated from sediment shrimp ponds. faecium Identification by Biochemical tests. Lactic fermentation for acid lactic against pathogen bacteria and mold 32 Enterococcus Isolated from sediment shrimp ponds. faecalis Identification by Biochemical tests. Lactic fermentation for acid lactic against pathogen bacteria and mold 33 Bifidobacterium Isolated from 3 days old baby meconium. longum Identification by Biochemical tests. Lactic fermentation for acid lactic against pathogen bacteria and mold 34 Bifidobacterium Isolated from 3 days old baby meconium. lactis Identification by Biochemical tests. Lactic fermentation for acid lactic against pathogen bacteria and mold 35 Bifidobacterium Isolated from human fermentation milk. breve Identification by Biochemical tests. Lactic fermentation for acid lactic against pathogen bacteria and mold 36 Bacillus clausii Isolated from soil. Identification by Biochemical tests. 37 Rhodopseudo- Isolated from waste water. Identification by monas Biochemical tests. Used in the hydrogen sulfide palustris (H2S) removal, promoting healthy biosphere for swiftlets, facilitating pairing up and nesting 38 Paenibacillus Isolated from sediment shrimp ponds. spp. Identification by Biochemical tests. Used in the NH3 oxidizing, promoting healthy biosphere for swiftlets, facilitating pairing up and nesting 39 Paenibacillus Isolated from fish pond. Identified by the 16S glycanilyticus rRNA sequence. 40 Paenibacillus Isolated from fish pond. Identified by the 16S pectinilyticus rRNA sequence. 41 Paenibacillus Isolated from fish pond. Identified by the 16S polymyxa rRNA sequence. 42 Paenibacillus Isolated from fish pond. Identified by the 16S xylanexedens rRNA sequence. 43 Paenibacillus Isolated from soil. tr{grave over (ô)}ng. Identified by the 16S endophyticus rRNA sequence. 44 Paenibacillus Isolated from soil casava starch factory. Identified amylolyticus by the 16S rRNA sequence. 45 Paenibacillus Isolated from soil casava starch factory. Identified glucanolyticus by the 16S rRNA sequence. 46 Paenibacillus Isolated from soil casava starch factory. Identified aceti by the 16S rRNA sequence. 47 Paenibacillus Isolated from soil. Identified by the 16S rRNA antibioticophila sequence. 48 Paenibacillus Isolated from soil casava starch factory. Identified cellulosilyticus by the 16S rRNA sequence. 49 Paenibacillus Isolated from fish pond. Identified by the 16S humicus rRNA sequence. 50 Paenibacillus Isolated from fish pond. Identified by the 16S xanthanilyticus rRNA sequence. 51 Paenibacillus Isolated from fish pond. Identified by the 16S chitinolyticus rRNA sequence. 52 Rhodococcus Isolated from sediment shrimp ponds. Identified spp. by the 16S rRNA sequence. Used in the hydrogen sulfide (H.sub.2S) removal, promoting healthy biosphere for swiftlets, facilitating pairing up and nesting 53 Rhodobacter Isolated from sediment shrimp ponds. Identified spp. by the 16S rRNA sequence. Used in the hydrogen sulfide (H.sub.2S) removal, promoting healthy biosphere for swiftlets, facilitating pairing up and nesting 54 Nitrosomonas Isolated from sediment shrimp ponds. Identified spp. by the 16S rRNA sequence. Used in nitrogen cycle to clean up ammonia (NH.sub.3). 55 Nitrobacter Isolated from sediment shrimp ponds. Identified spp. by the 16S rRNA sequence. Used in nitrogen cycle to clean up nitrite (N—NO.sub.2). 56 Paracoccus Isolated from sediment shrimp ponds. Identified denitrificans by the 16S rRNA sequence. Used in the hydrogen sulfide (H.sub.2S) removal, NH3 oxidizing, promoting healthy biosphere for swiftlets, facilitating pairing up and nesting
[0049] Continuing with step 103, in some aspects of the present invention, some functional yeasts and additional bacteria are added to the mixture of step 102.
TABLE-US-00002 No. Microorganisms Description 1 Pichia anomala Isolated from waste water vegetable oil factory. Identified by biochemial test. Biosyntheis antimold factors. Biosyntheis ethyl acetate promoting healthy biosphere for swiftlets. 2 Saccharomyces Isolated from stater yeast cookies cerevisiae fruit wastes. Identified by biochemial test. Alcohool fermentation. 3 Rhodotorula Isolated from fruit waste. Identification by glutinis Biochemical tests. Alcohol fermentation.
[0050] Next, at step 104, the beneficial organisms including bacteria and yeasts are then encapsulated in a slow-releasing carrier such as nanozeolite, maize starch, cassava starch, and zeolite. These slow-releasing carriers (base) have pores and channels within their crystal structure impregnated with the above beneficial bacteria and yeasts listed in Table 1 and Table 2.
[0051] The process 100 disclosed above achieved the following objectives:
[0052] Developed a formulation of microorganisms including bacteria and yeasts that improve the living conditions for a particular farm animals using biotechnology approach.
[0053] Facilitate swiftlets to pair up and to nest.
[0054] Eliminate ammonia (NH.sub.3) in swiftlet feces.
[0055] Eliminate hydrogen sulfide (H.sub.2S).
[0056] Reduce the bad odor lingering in the bird houses.
[0057] Next referring to
[0058] At step 201, a slow-releasing carrier is prepared. In many aspects of the present invention, neozolites or the likes such as nanozeolites, zeolites, cassava starch, maize starch, etc. was selected to be the slow-releasing carrier because of its advantageous physical and chemical characteristics. In some embodiments of the present invention, nanozeolites were prepared by using a simple co-precipitation method. At first, sodium silicate solution (73.3 g/100 ml of distilled water) and 8.33 ml of ethylene glycol were mixed in a three-necked round bottom flask. Further, the flask was fitted with a reflux condenser and dropping funnels. Then, the mixture was stirred for 30 min with a magnetic stirrer at a temperature of 50-60° C. Next, a pre-prepared aluminum sulfate solution (26.23 g/83 ml) and sodium hydroxide solution (10 g/83 ml) were added dropwise while stirring and maintaining the same temperature. After completing the reaction, the pH level of the solution was neutralized. Later, the sample was filtered, dried at 105° C. and finally was annealed at 650° C. to obtain the desired porous grey colored zeolite. In various embodiments of the present invention, step 201 is realized by grinding and sieving zeolites to obtain nanozeolites.
[0059] At step 202, a mixture of beneficial microorganisms to animal farms are prepared, In many aspects of the present invention, beneficial microorganisms include both antagonistic and preventive microorganisms that not only eliminate all pathogens, toxic chemicals such as ammonium, hydrogen sulfide, nitrites but also improve all living conditions in the animal farms. In many aspects of the present invention, these microorganisms were mixed using a specific percentage by weight (% w/w) or colony-forming unit per kilogram (CFU/kg) or CFU per liter (CFU/I). Table 3 below lists the beneficial microorganisms including bacteria and yeasts used for swiftlet bird houses.
TABLE-US-00003 TABLE 1 Lists of Beneficial Microorganisms and Their Relative Percentage Weight (% w/w) or CFU/kg. No. Microorganism Quantity (CFU/kg) 1 Paenibacillus favisporus 10.sup.10-10.sup.13 2 Bacillus stratosphericus 10.sup.10-10.sup.13 3 Paenibacilus typhea 10.sup.10-10.sup.13 4 Bacillus aryabhattai: 10.sup.10-10.sup.13 5 Fictibacillus phosphorivorans 10.sup.10-10.sup.13 6 Bacillus xiamenensis 10.sup.10-10.sup.13 7 Bacillus megaterium 10.sup.10-10.sup.13 8 Bacillus altitudinis 10.sup.10-10.sup.13 9 Bacillus velezensis 10.sup.10-10.sup.13 10 Bacillus amyloliquefaciens 10.sup.10-10.sup.13 11 Baccillus subtilis 10.sup.10-10.sup.13 12 Baccillus licheniformis 10.sup.10-10.sup.13 13 Bacillus mensentericus 10.sup.10-10.sup.13 14 Bacillus pumilus 10.sup.10-10.sup.13 15 Bacillus coagulans 10.sup.10-10.sup.13 16 Bacillus thuringiensis 10.sup.10-10.sup.13 17 Bacillus firmus 10.sup.10-10.sup.13 18 Bacillus aryabhattai 10.sup.10-10.sup.13 19 Lactobacillus 10.sup.10-10.sup.13 acidophillus 20 Lactobacillus plantarum 10.sup.10-10.sup.13 21 Lactobacillus caseii 10.sup.10-10.sup.13 22 Lactobacillus fermentum 10.sup.10-10.sup.13 23 Streptococcus thermophilus 10.sup.10-10.sup.13 24 Pediococcus acidilactici 10.sup.10-10.sup.13 25 Lactococcus lactis 10.sup.10-10.sup.13 26 Lactobacillus rhamnosus 10.sup.10-10.sup.13 27 Lactobacillus reuteri 10.sup.10-10.sup.13 28 Lactobacillus paracasei 10.sup.10-10.sup.13 29 Lactobacillus helviticus 10.sup.10-10.sup.13 30 Lactobacillus grasseri 10.sup.10-10.sup.13 31 Enterococcus faecium 10.sup.10-10.sup.13 32 Enterococcus faecalis 10.sup.10-10.sup.13 33 Bifidobacterium longum 10.sup.10-10.sup.13 34 Bifidobacterium lactis 10.sup.10-10.sup.13 35 Bifidobacterium breve 10.sup.10-10.sup.13 36 Bacillus clausii 10.sup.10-10.sup.13 37 Rhodopseudomonas palustris 10.sup.10-10.sup.13 38 Paenibacillus spp. 10.sup.10-10.sup.13 39 Paenibacillus glycanilyticus 10.sup.10-10.sup.13 40 Paenibacillus pectinilyticus 10.sup.10-10.sup.13 41 Paenibacillus polymyxa 10.sup.10-10.sup.13 42 Paenibacillus xylanexedens 10.sup.10-10.sup.13 43 Paenibacillus endophyticus 10.sup.10-10.sup.13 44 Paenibacillus amylolyticus 10.sup.10-10.sup.13 45 Paenibacillus glucanolyticus 10.sup.10-10.sup.13 46 Paenibacillus aceti 10.sup.10-10.sup.13 47 Paenibacillus antibioticophila 10.sup.10-10.sup.13 48 Paenibacillus cellulosilyticus 10.sup.10-10.sup.13 49 Paenibacillus humicus 10.sup.10-10.sup.13 50 Paenibacillus xanthanilyticus 10.sup.10-10.sup.13 51 Paenibacillus chitinolyticus 10.sup.10-10.sup.13 52 Rhodococcus spp. 10.sup.10-10.sup.13 53 Rhodobacter spp. 10.sup.10-10.sup.13 54 Nitrosomonas spp. 10.sup.10-10.sup.13 55 Nitrobacter spp. 10.sup.10-10.sup.13 56 Paracoccus denitrificans 10.sup.10-10.sup.13 57 Pichia anomala 10.sup.10-10.sup.13 58 Saccharomyces cerevisiae 10.sup.10-10.sup.13 59 Rhodotorula glutinis 10.sup.10-10.sup.13
[0060] It is noted that the list of yeasts and bacteria in Table 3 is for swiftlet bird house. The composition for other type of animal farms with different bacteria/yeast mixture of different percentage by weight (% w/w) is within the scope of the present invention.
[0061] At step 203, the bacteria and yeasts listed in step 202 are encapsulated in the slow-releasing carriers such as nanozeolite to form the composition (solution/formula). The composition (solution/formula) was prepared by impregnating a mixture of bacteria into nanozeolites. In some embodiments of the present invention, the sample was multiplied using a Polymerase Chain Reaction (PCR) or the likes, which was then separated using a Flotweg decanter centrifuges machine with a 0.2 μm filter screen. The Room-temperature nanozeolite were mixed with water and the mixture of bacteria and yeasts were added and the composition was stirred for 3 hours. Finally, the solution was filtered, cold-dried, or vacuum dried at the temperature less than 40° C. and blended at 12,000 rpm to get fine ground particles of composition (solution/formula) that was then stored in an airtight container to stay dry. Every kilo of the powder composition is mixed with 100 liters of water before use.
[0062] Next referring to
[0063] At step 301, a specific type of animal farm is selected. In many aspects of the present invention, animal farms of step 301 include but not limited to swiftlet (Aerodramus fuciphagus, Aerodramus maximus, and Aerodramus spodiopygius) bird houses, poultry farms, enclosed cattle farms, fish and shrimp farms in aquatic ponds and nurseries.
[0064] At step 302, whether the animal farms are aquatic farms (aquafarms) or other type of aquatic surgeries and/or ponds are determined. Step 302 is implemented by determining whether the animals are aquatic creatures such as fishes, eels, frogs, and shrimps.
[0065] At step 303, if the farm animals are not aquatic, an appropriate composition (solution/formula) is selected, In many aspects of the present invention, the formula as described in method 200 is used for swiftlet bird houses.
TABLE-US-00004 TABLE 4 List of Beneficial Microorganism formula used in Fowl and Cattle Farms. Quantity No. Microorganism Description (CFU/kg) 1 Paenibacillus Isolated from catfish pond 10.sup.10-10.sup.13 favisporus sediment. Identified by the 16S rRNA sequence. Used in nitrogen cycle to clean up ammonia (NH.sub.3). 2 Bacillus Isolated from catfish pond 10.sup.10-10.sup.13 stratosphericus sediment. Identified by the 16S rRNA sequence. Used to hydrolysis starch, biodegradable cellulose. 3 Peanibacilus Isolated from catfish pond 10.sup.10-10.sup.13 typhea sediment. Identified by the 16S rRNA sequence. 4 Bacillus Isolated from catfish pond 10.sup.10-10.sup.13 aryabhattai: sediment. Identified by the 16S rRNA sequence. Used to hydrolysis starch, biodegradable cellulose. 5 Fictibacillus Isolated from catfish pond 10.sup.10-10.sup.13 phosphorivorans sediment, Identified by the 16S rRNA sequence.. 6 Bacillus Isolated from catfish pond 10.sup.10-10.sup.13 xiamenensis sediment. Used to hydrolysis protein. Used to generate healthy environment for cattle, poultry barns. 7 Bacillus Isolatede from shrimp pond 10.sup.10-10.sup.13 megaterium sediment. Identified by the 16S rRNA sequence. Used for phosho soluble. 8 Bacillus altitudinis Isolated from agriculture soil. 10.sup.10-10.sup.13 Identified by the 16S rRNA sequence. Used to clean up excess metal ions. Used to hydrolysis starch. 9 Bacillus velezensis Isolated from catfish pond 10.sup.10-10.sup.13 sediment. Identified by the 16S rRNA sequence. Used to hydrolysis starch, protein; promoting healthy biosphere for poultry, cattle barns. 10 Bacillus Isolated from rhizosphere soil 10.sup.10-10.sup.13 amyloliquefaciens of legum. Identified by the 16S rRNA sequence. Used to hydrolysis starch, biosynthesis biofloculant. 10 Baccillus subtilis Isolated from many sources. 10.sup.10-10.sup.13 Biosynthesis enzyme amylase, protease, lipase. Protease, bacteriocine 13 Baccillus Isolated from sediment shrimp 10.sup.10-10.sup.13 licheniformis ponds. Biosyntheis protease, lipase to degradation organic material promoting healthy biosphere for poultry, cattle barns. 13 Bacillus Isolated from sediment shrimp 10.sup.10-10.sup.13 mensentericus ponds. Biosyntheis amylase breakdown starch, lipase to degradation organic material promoting healthy biosphere for poultry, cattle barns. 14 Bacillus pumilus Isolated from sediment shrimp 10.sup.10-10.sup.13 ponds. Biosynthesis enzyme amylase, biofluculant. Rapid degradation of organic material in poultry, cattle barns. 15 Bacillus coagulans Isolated from food lactic 10.sup.10-10.sup.13 fermentation. Lactic fermentation and biosynthesis bacteriocine 16 Bacillus Isolated from soil. Biosynthesis 10.sup.10-10.sup.13 thuringiensis endotoxin to kill pests. 17 Bacillus firmus Isolated from soil. Biosynthesis 10.sup.10-10.sup.13 protease 18 Bacillus Isolated from soil. Biosynthesis 10.sup.10-10.sup.13 aryabhattai enzyme cellulase, seaweeds biofluculant 19 Lactobacillus Isolated from food 10.sup.10-10.sup.13 acidophillus fermentation. Identification by Biochemical tests. Biosynthesis bacteriocine and lactic fermentation for acid lactic against pathogen bacteria and mold. 20 Lactobacillus Isolated from pickle. 10.sup.10-10.sup.13 plantarum Identification by Biochemical tests. Biosynthesis bacteriocine and lactic fermentation for acid lactic against pathogen bacteria and mold 21 Lactobacillus Isolated from natural 10.sup.10-10.sup.13 caseii fermentation milk. Identification by Biochemical tests. Biosynthesis bacteriocine and Biosynthesis bacteriocine and lactic fermentation for acid lactic against pathogen bacteria and mold 22 Lactobacillus Isolated from food fermentation 10.sup.10-10.sup.13 fermentum lactic. Identification by Biochemical tests. Biosynthesis bacteriocine and lactic fermentation for acid lactic against pathogen bacteria and mold 23 Streptococcus Isolated from food fermentation 10.sup.10-10.sup.13 thermophilus lactic. Identification by Biochemical tests. Biosynthesis bacteriocine and lactic fermentation for acid lactic against pathogen bacteria and mold 24 Pediococcus Isolated from food fermentation 10.sup.10-10.sup.13 acidilactici lactic. Identification by Biochemical tests. Biosynthesis bacteriocine and lactic fermentation for acid lactic against pathogen bacteria and mold 25 Lactococcus lactis Isolated from food fermentation 10.sup.10-10.sup.13 lactic. Identification by Biochemical tests. Biosynthesis bacteriocine and lactic fermentation for acid lactic against pathogen bacteria and mold 26 Lactobacillus Isolated from food fermentation 10.sup.10-10.sup.13 rhamnosus lactic. Identification by Biochemical tests. Biosynthesis bacteriocine and lactic fermentation for acid lactic against pathogen bacteria and mold 27 Lactobacillus Isolated from food fermentation 10.sup.10-10.sup.13 reuteri lactic. Identification by Biochemical tests. Biosynthesis bacteriocine and lactic fermentation for acid lactic against pathogen bacteria and mold 28 Lactobacillus Isolated from food fermentation 10.sup.10-10.sup.13 paracasei lactic. Identification by Biochemical tests. Biosynthesis bacteriocine and lactic fermentation for acid lactic against pathogen bacteria and mold 29 Lactobacillus Isolated from food fermentation 10.sup.10-10.sup.13 helviticus lactic. Identification by Biochemical tests. Biosynthesis bacteriocine and lactic fermentation for acid lactic against pathogen bacteria and mold 30 Lactobacillus Isolated from food fermentation 10.sup.10-10.sup.13 grasseri lactic. Identification by Biochemical tests. Biosynthesis bacteriocine and lactic fermentation for acid lactic against pathogen bacteria and mold 31 Enterococcus Isolated from sediment shrimp 10.sup.10-10.sup.13 faecium ponds. Identification by Biochemical tests. Lactic fermentation for acid lactic against pathogen bacteria and mold 32 Enterococcus Isolated from sediment shrimp 10.sup.10-10.sup.13 faecalis ponds. Identification by Biochemical tests. Lactic fermentation for acid lactic against pathogen bacteria and mold 33 Bifidobacterium Isolated from 3 days old baby 10.sup.10-10.sup.13 longum meconium. Identification by Biochemical tests. Lactic fermentation for acid lactic against pathogen bacteria and mold 34 Bifidobacterium Isolated from 3 days old baby 10.sup.10-10.sup.13 lactis meconium. Identification by Biochemical tests. Lactic fermentation for acid lactic against pathogen bacteria and mold 35 Bifidobacterium Isolated from human 10.sup.10-10.sup.13 breve fermentation milk. Identification by Biochemical tests. Lactic fermentation for acid lactic against pathogen bacteria and mold 36 Bacillus clausii Isolated from soil. Identification 10.sup.10-10.sup.13 by Biochemical tests. 37 Rhodopseudomonas Isolated from waste water. 10.sup.10-10.sup.13 palustris Identification by Biochemical tests. Used in the hydrogen sulfide (H.sub.2S) removal, promoting healthy biosphere for poultry, cattle barns. 38 Paenibacillus spp. Isolated from sediment shrimp 10.sup.10-10.sup.13 ponds. Identification by Biochemical tests. Used in the NH3 oxidizing, bio-degradation organic material, promoting healthy biosphere for poultry, cattle barns. 39 Paenibacillus Isolated from fish pond. 10.sup.10-10.sup.13 glycanilyticus Identified by the 16S rRNA sequence. Bio-degradation organic material. 40 Paenibacillus Isolated from fish pond. 10.sup.10-10.sup.13 pectinilyticus Identified by the 16S rRNA sequence. Bio-degradation organic material. 41 Paenibacillus Isolated from fish pond. 10.sup.10-10.sup.13 polymyxa Identified by the 16S rRNA sequence. Bio-degradation organic material. 42 Paenibacillus Isolated from fish pond. 10.sup.10-10.sup.13 xylanexedens Identified by the 16S rRNA sequence. Bio-degradation organic material. 43 Paenibacillus Isolated from soil. trông. 10.sup.10-10.sup.13 endophyticus Identified by the 16S rRNA sequence. Bio-degradation organic material. 44 Paenibacillus Isolated from soil casava 10.sup.10-10.sup.13 amylolyticus starch factory. Identified by the 16S rRNA sequence. Bio- degradation organic material. 45 Paenibacillus Isolated from soil casava 10.sup.10-10.sup.13 glucanolyticus starch factory. Identified by the 16S rRNA sequence. Bio- degradation organic material. 46 Paenibacillus aceti Isolated from soil casava 10.sup.10-10.sup.13 starch factory. Identified by the 16S rRNA sequence. Bio- degradation organic material. 47 Paenibacillus Isolated from soil. Identified by 10.sup.10-10.sup.13 antibioticophila the 16S rRNA sequence. Bio- degradation organic material. 48 Paenibacillus Isolated from soil casava 10.sup.10-10.sup.13 cellulosilyticus starch factory. Identified by the 16S rRNA sequence. 49 Paenibacillus Isolated from fish pond. 10.sup.10-10.sup.13 humicus Identified by the 16S rRNA sequence. Bio-degradation organic material. 50 Paenibacillus Isolated from fish pond. 10.sup.10-10.sup.13 xanthanilyticus Identified by the 16S rRNA sequence. Bio-degradation organic material. 51 Paenibacillus Isolated from fish pond. 10.sup.10-10.sup.13 chitinolyticus Identified by the 16S rRNA sequence. Bio-degradation organic material. 52 Rhodococcus spp. Isolated from sediment shrimp 10.sup.10-10.sup.13 ponds. Identified by the 16S rRNA sequence. Used in the hydrogen sulfide (H.sub.2S) removal. promoting healthy biosphere for poultry, cattle barns. 53 Rhodobacter spp. Isolated from sediment shrimp 10.sup.10-10.sup.13 ponds. Identified by the 16S rRNA sequence. Used in the hydrogen sulfide (H.sub.2S) removal. promoting healthy biosphere for poultry, cattle barns. 54 Nitrosomonas spp. Isolated from sediment shrimp 10.sup.10-10.sup.13 ponds. Identified by the 16S rRNA sequence. Used in nitrogen cycle to clean up ammonia (NH.sub.3). 55 Nitrobacter spp. Isolated from sediment shrimp 10.sup.10-10.sup.13 ponds. Identified by the 16S rRNA sequence. Used in nitrogen cycle to clean up nitrite (N—NO.sub.2). 56 Paracoccus Isolated from sediment shrimp 10.sup.10-10.sup.13 denitrificans ponds. Identified by the 16S rRNA sequence. Used in the hydrogen sulfide (H.sub.2S) removal. NH3 oxidizing, promoting healthy biosphere for poultry, cattle barns. 57 Pichia anomala Isolated from waste water 10.sup.10-10.sup.13 vegetable oil factory. Identified by biochemical test. Biosynthesis antimold factors. Biosynthesis ethyl acetate promoting healthy biosphere for poultry, cattle barns.. 58 Saccharomyces Isolated from yeast cookies, 10.sup.10-10.sup.13 cerevisiae fruit waste. Identified by biochemical test. Alcohol fermentation. 59 Rhodotorula Isolated from fruit waste. 10.sup.10-10.sup.13 glutinis Identification by Biochemical tests. Alcohol fermentation. 60 Aspergiullus oryzae Isolated from soya 10.sup.10-10.sup.13 fermentation process, Identification by morphology. 61 Aspergillus nigrer Isolated from lime, 10.sup.10-10.sup.13 Identification by morphology. 62 Streptomyces spp. Isolated from forest, 10.sup.10-10.sup.13 Identification by morphology. 63 Paecillomyces spp. Isolated from agriculture soi1. 10.sup.10-10.sup.13 Identification by morphology. 64 Metarhizium spp. Isolated from agriculture soi1. 10.sup.10-10.sup.13 Identification by morphology. 65 Beauveria spp. Isolated from agriculture soi1. 10.sup.10-10.sup.13 Identification by morphology.
[0066] At step 304, one kilogram of composition (solution/formula) powder is mixed well with 100 liters of water.
[0067] At step 305, the solution is applied thoroughly to the animal farms. In various aspects of the present invention, the microorganism solution is sprayed directly onto the surrounding walls of the animal farms. In some aspects of the present invention, the animal farm is cleaned and ridded of droppings and unwanted materials such as feathers and dusts. Then, the solution in step 304 is sprayed on the floors and the surrounding walls of the animal farms. In case of the swiftlet bird houses, bird drops or guano are removed, the grounds, the ceilings, and the surroundings on every floor are scrubbed cleaned and then the mixture of step 304 are sprayed thoroughly. Then, the symbiotic relationships between the beneficial organisms including antagonistic and other environment improving yeasts and bacteria listed in Table 1 to Table 5 above and the pathogenic agents are allowed to happen. In the nitrogen cycle, the following reactions will occur:
2NH.sub.3+H.sub.2O.fwdarw.2NH.sub.4
NH.sub.4.sup.++2e.fwdarw.NH.sub.2OH.fwdarw.NO.sub.2
NO.sub.2.sup.−+½O.sub.2—.fwdarw.NO.sub.3
[0068] On the other hand, in swiftlet bird houses and other farm animals, the level of hydrogen sulfide (H.sub.2S) levels may be as high as 100 ppb. As noted in Tables above, some listed bacterium are sulfide oxidizing bacteria (SOB). The oxidation reaction occurs in the following chemical reaction: H.sub.2S+½O.sub.2.fwdarw.SO.sub.4.sup.2−+2H.sup.30 .
[0069] At step 306, if the animal farm is an aquafarm for fishes, eels, or the likes, step 306 is performed in two stages. At step 306A, an appropriate formula for aquafarms is selected, similar to step 303. Ingredients of the farm animals applicable to aquafarms are shown in Table 5 below.
TABLE-US-00005 TABLE 5 Formula for Aquatic Culture (Aquaculture) No. Microorganism Description 1 Paenibacillus Isolated from catfish pond sediment. Identified favisporus by the 16S rRNA sequence. Used in nitrogen cycle to clean up ammonia (NH.sub.3). 2 Bacillus Isolated from catfish pond sediment. Identified stratosphericus by the 16S rRNA sequence. Used to hydrolysis starch, biodegradable cellulose. 3 Peanibacilus Isolated from catfish pond sediment. Identified typhea by the 16S rRNA sequence. 4 Bacillus Isolated from catfish pond sediment. Identified aryabhattai: by the 16S rRNA sequence. Used to hydrolysis starch, biodegradable cellulose. 5 Fictibacillus Isolated from catfish pond sediment, Identified phosphorivarans by the 16S rRNA sequence. 6 Bacillus Isolated from catfish pond sediment. Used to xiamenensis hydrolysis protein. Used to generate healthy environment for aquaculture. 7 Bacillus Isolatede from shrimp pond sediment. Identified megaterium by the 16S rRNA sequence. Used for phosphor soluble. 8 Bacillus Isolated from agriculture soil. Identified by the altitudinis 16Sr RNA sequence. Used to clean up excess metal ions. Used to hydrolysis starch. 9 Bacillus Isolated from catfish pond sediment. Identified velezensis by the 16S rRNA sequence. Used to hydrolysis starch, protein; promoting healthy biosphere for aquaculture. 10 Bacillus Isolated from rhizosphere soil of legumes. amylolique- Identified by the 16S rRNA sequence. Used to faciens hydrolysis starch, biosynthesis biofloculant. 11 Baccillus Isolated from many sources. Biosynthesis enzyme subtilis amylase, protease, lipase. Protease, bacteriocine 12 Baccillus Isolated from sediment shrimp ponds. licheniformis Biosynthesis protease, lipase to degradation organic material promoting healthy biosphere for aquaculture. 13 Bacillus Isolated from sediment shrimp ponds. mensentericus Biosynthesis amylase breakdown starch lipase to degradation organic material promoting healthy biosphere for aquaculture. 14 Bacillus pumilus Isolated from sediment shrimp ponds. Biosynthesis enzyme amylase, biofluculant. Rapid degradation of organic material. 15 Bacillus Isolated from food lactic fermentation. Lactic coagulans fermentation and biosynthesis bacteriocine 16 Bacillus Isolated from soil. Biosynthesis endotoxin to thuringiensis kill pests. 17 Bacillus firmus Isolated from soil. Biosynthesis protease 18 Bacillus Isolated from soil. Biosynthesis enzyme aryabhattai cellulase, seaweed biofluculant 19 Lactobacillus Isolated from food fermentation. Identification acidophillus by Biochemical tests. Biosynthesis bacteriocine and lactic fermentation for acid lactic against pathogen bacteria and mold. 20 Lactobacillus Isolated from pickle. Identification by plantarum Biochemical tests. Biosynthesis bacteriocine and lactic fermentation for acid lactic against pathogen bacteria and mold 21 Lactobacillus Isolated from natural fermentation milk. caseii Identification by Biochemical tests. Biosynthesis bacteriocine and Biosynthesis bacteriocine and lactic fermentation for acid lactic against pathogen bacteria and mold 22 Lactobacillus Isolated from food fermentation lactic. fermentum Identification by Biochemical tests. Bio- synthesis bacteriocine and lactic fermentation for acid lactic against pathogen bacteria and mold 23 Streptococcus Isolated from food fermentation lactic. thermophilus Identification by Biochemical tests. Bio- synthesis bacteriocine and lactic fermentation for acid lactic against pathogen bacteria and mold 24 Pediococcus Isolated from food fermentation lactic. acidilactici Identification by Biochemical tests. Bio- synthesis bacteriocine and lactic fermentation for acid lactic against pathogen bacteria and mold 25 Lactococcus Isolated from food fermentation lactic. lactis Identification by Biochemical tests. Bio- synthesis bacteriocine and lactic fermentation for acid lactic against pathogen bacteria and mold 26 Lactobacillus Isolated from food fermentation lactic. rhamnosus Identification by Biochemical tests. Bio- synthesis bacteriocine and lactic fermentation for acid lactic against pathogen bacteria and mold 27 Lactobacillus Isolated from food fermentation lactic. reuteri Identification by Biochemical tests. Bio- synthesis bacteriocine and lactic fermentation for acid lactic against pathogen bacteria and mold 28 Lactobacillus Isolated from food fermentation lactic. paracasei Identification by Biochemical tests. Bio- synthesis bacteriocine and lactic fermentation for acid lactic against pathogen bacteria and mold 29 Lactobacillus Isolated from food fermentation lactic. helviticus Identification by Biochemical tests. Bio- synthesis bacteriocine and lactic fermentation for acid lactic against pathogen bacteria and mold 30 Lactobacillus Isolated from food fermentation lactic. grasseri Identification by Biochemical tests. Bio- synthesis bacteriocine and lactic fermentation for acid lactic against pathogen bacteria and mold 31 Enterococcus Isolated from sediment shrimp ponds. faecium Identification by Biochemical tests. Lactic fermentation for acid lactic against pathogen bacteria and mold 32 Enterococcus Isolated from sediment shrimp ponds. faecalis Identification by Biochemical tests. Lactic fermentation for acid lactic against pathogen bacteria and mold 33 Bifidobacterium Isolated from 3 days old baby meconium. longum Identification by Biochemical tests. Lactic fermentation for acid lactic against pathogen bacteria and mold 34 Bifidobacterium Isolated from 3 days old baby meconium. lactis Identification by Biochemical tests. Lactic fermentation for acid lactic against pathogen bacteria and mold 35 Bifidobacterium Isolated from human fermentation milk. breve Identification by Biochemical tests. Lactic fermentation for acid lactic against pathogen bacteria and mold 36 Bacillus clausii Isolated from soil. Identification by Biochemical tests. 37 Rhodo- Isolated from waste water. Identification by pseudomonas Biochemical tests. Used in the hydrogen sulfide palustris (H.sub.2S) removal. promoting healthy biosphere for aquaculture. 38 Paenibacillus Isolated from sediment shrimp ponds. spp. Identification by Biochemical tests. Used in the NH3 oxidizing, promoting healthy biosphere for aquaculture. 39 Paenibacillus Isolated from fish pond. Identified by the glycanilyticus 16S rRNA sequence. 40 Paenibacillus Isolated from fish pond. Identified by the pectinilyticus 16S rRNA sequence. 41 Paenibacillus Isolated from fish pond. Identified by the polymyxa 16S rRNA sequence. 42 Paenibacillus Isolated from fish pond. Identified by the xylanexedens 16S rRNA sequence. 43 Paenibacillus Isolated from soil. tr ng. Identified by the endophyticus 16S rRNA sequence. 44 Paenibacillus Isolated from soil casava starch factory. amylolyticus Identified by the 16S rRNA sequence. 45 Paenibacillus Isolated from soil casava starch factory. glucanolyticus Identified by the 16S rRNA sequence. 46 Paenibacillus Isolated from soil casava starch factory. aceti Identified by the 16S rRNA sequence. 47 Paenibacillus Isolated from soil. Identified by the 16S rRNA antibioticophila sequence. 48 Paenibacillus Isolated from soil casava starch factory. cellulosilyticus Identified by the 16S rRNA sequence. 49 Paenibacillus Isolated from fish pond. Identified by the humicus 16S rRNA sequence. 50 Paenibacillus Isolated from fish pond. Identified by the xanthanilyticus 16S rRNA sequence. 51 Paenibacillus Isolated from fish pond. Identified by the chitinolyticus 16S rRNA sequence. 52 Rhodococcus Isolated from sediment shrimp ponds. Identified spp. by the 16S rRNA sequence. Used in the hydrogen sulfide (H.sub.2S) removal. promoting healthy biosphere for aquaculture 53 Rhodobacter Isolated from sediment shrimp ponds. Identified spp. by the 16S rRNA sequence. Used in the hydrogen sulfide (H.sub.2S) removal, promoting healthy biosphere for aquaculture. 54 Nitrosomonas Isolated from sediment shrimp ponds. Identified spp. by the 16S rRNA sequence. Used in nitrogen cycle to clean up ammonia (NH.sub.3). 55 Nitrobacter spp. Isolated from sediment shrimp ponds. Identified by the 16S rRNA sequence. Used in nitrogen cycle to clean up nitrite (N—NO.sub.2). 56 Paracoccus Isolated from sediment shrimp ponds. Identified denitrificans by the 16S rRNA sequence. Used in the hydrogen sulfide (H.sub.2S) removal, NH3 oxidizing, promoting healthy biosphere for aquaculture. 57 Pichia anomala Isolated from waste water vegetable oil factory. Identified by biochemical test. 58 Saccharomyces Isolated from yeast cookies, fruit wastes. cerevisiae Identified by biochemical test. 59 Rhodotorula Isolated from fruit waste. Identification by glutinis Biochemical tests.
[0070] At step 306B where step 304 is skipped, the selected formula for aquafarms in Table 5 is applied directly to the aquatic farms.
[0071] Thus, process 300 of the present disclosure achieves the following objectives:
[0072] (a) Eliminate the excess toxic ammonia (NH.sub.3) and convert them into nitrate (NO.sub.3.sup.−) that are beneficial to plants and animals since plants and animals cannot get nitrogen (N.sub.2) directly from the environment. They get the essential nitrogen from nitrate ions (NO.sub.3.sup.−).
[0073] (b) Eliminate hydrogen sulfide (H.sub.2S) trapped in the bird house;
[0074] (c) Get rid of the bad odors lingering in the bird houses;
[0075] (d) Decompose swiftlets guano.
[0076] Next referring to
[0077] Swiftlet bird house 400 typically has a rectangular structure with a plurality of floors (levels) 401-403. A roof 404 covers the top the rectangular structure. A set of foundation and footings 405 are used to support bird house 400 from the bottom side. A first ground (platform) on ground floor 401 may be covered with a first removable rug 406. A second ground (platform) on first floor 402 may be covered with a second removable rug 407. A second ground (platform) on second floor 403 may be covered with a third removable rug 408. First to third removable rug 406 to 408 may be made of graphene or guano-doped graphene. During clean-up, these removable rugs 406-408 can be removed to collect the guano. A small window 409—usually very narrow—is for exit and entrance of the swiftlets. A computer box 411 is placed either inside or away from bird house 400 contains speaker controller and/or a computer to receive information from sensors 421 that carefully placed to collect the bio information. A series of speakers 412 are placed on the surrounding walls to attract the swiftlets. Sensors 421 are served as crowd-sensing including temperatures sensors, cameras, air/gas detectors, chemical, mirrors, and bacteria sensors. Sensors 421 send information to a central processing unit (CPU) (not shown) inside computer box 411. In some embodiments, sensors 421 send information to a portable devices such as smart phones, laptops, or tablets.
[0078] Finally, referring to
[0079] At step 401, a formula for swiftlet bird houses composition (solution/formula) is prepared. In many aspects of the present invention, the composition (solution/formula) contains the exact percentage weigh (% w/w) since overabundance of a particular bacteria may be detrimental to swiftlet bird house 400. Step 401 is realized by the composition (solution/formula) containing the components as listed in Table 3. In some aspects of the present invention, the composition (solution/formula) is freeze-dried to create a powder formula.
[0080] At step 402, every kilo of the composition (solution/formula) is mixed with 100 liters of water. In some aspects of the present invention gel and liquid form of the composition are used.
[0081] At step 403, the swiftlet bird house is sprayed thoroughly with the mixture of step 402 above. In practice, step 403 is realized by cleaning up swiftlet bird house 400 and sprayed the surrounding walls of ground floor 401, first floor 402, and second floor 403 as well as ceiling and roof 404. In some aspects of the present invention, the animal farms can be newly built or existing buildings made of either cement, bricks, or woods. The compositions in the form of gel, liquid, solid, and/or power are within the scope of the present invention.
[0082] In summary, the novel compositions of the present invention as disclosed above achieve the following objectives:
[0083] A total microorganism solution (solution/formula) to improve the living conditions in animal farm houses, especially swiftlet bird houses.
[0084] a microorganism composition (solution/formula) capable of decomposing swiftlet droppings (guano) in the bird houses to prevent toxic gases and other polluting agents from accumulating.
[0085] a microorganism composition (solution/formula) capable of helping said bird's nests to be healthy white color;
[0086] a microorganism composition (solution/formula) capable of helping swiftlets to stay healthy, increasing the number of bird nests whose fibers are sturdy, flexible, long-stranded, and unbroken.
[0087] a microorganism composition (solution/formula) capable of preventing insects such as aunts, mites, and bugs from killing the swiftlet newly born chicks;
[0088] a microorganism composition (solution/formula) capable of preventing fungi from growing on the nest supporting wooden bars, on the surrounding walls, and on the floors.
[0089] a microorganism composition (solution/formula) capable of preventing parasites from growing from the accumulated swiftlet's droppings (guano) that can adversely affect the swiftlets and their nests.
[0090] a microorganism composition (solution/formula) capable of attracting the swiftlets to come dwelling inside the bird houses;
[0091] a microorganism composition (solution/formula) capable of keeping the swiftlets from leaving the bird houses;
[0092] a microorganism composition (solution/formula) capable of causing the swiftlets to pair up and to nest in the bird houses;
[0093] a microorganism composition (solution/formula) capable of making the newly born swiftlet chicks to feel comfortable in their nests;
[0094] a microorganism composition (solution/formula) capable of keeping the swiftlet chicks to stay and to nest in their bird houses where they were born;
[0095] a microorganism composition (solution/formula) capable of increasing the number of bird's nests per bird house;
[0096] a microorganism composition (solution/formula) capable of shortening the edible bird's nests (EBN) harvesting time;
[0097] a microorganism composition (solution/formula) capable of lengthening the durability and the longevity of the bird houses;
[0098] a microorganism composition capable (solution/formula) of stimulating the reproduction of the swiftlets;
[0099] a microorganism composition (solution/formula) capable of eliminating the new cement odors of a newly built bird house;
[0100] a microorganism composition (solution/formula) capable of eliminating the smells of new woods inside the bird houses that dispel the swiftlets;
[0101] a microorganism composition (solution/formula) capable of eliminating the rotten smells of the molds and fungi in the old swiftlet houses, improving the living conditions of the existing and old swiftlet houses;
[0102] a microorganism composition (solution/formula) capable of quickly stabilizing the quality air inside newly built bird houses;
[0103] a microorganism composition (solution/formula) capable of reducing stresses to swiftlets;
[0104] a microorganism composition (solution/formula) capable of recreating a healthy and conducive environment to the swiftlets;
[0105] a microorganism composition (solution/formula) capable of eliminating hydrogen sulfide (H.sub.2S) and nitrite (NO.sub.3.sup.−), ammonia (NH.sub.3), and nitrate (NO.sub.2.sup.−) accumulated over time inside the bird houses;
[0106] a microorganism composition (solution/formula) capable of increasing the nutritional values and proteins in the edible bird's nests (EBN).
[0107] a microorganism composition (solution/formula) capable of decomposing animal manure after being collected from animal farms such as barns, bird houses, fowl houses to minimize pollution to the surrounding environment and the final product can be used as a safe fertilizer for trees, plants, and the green environment.
[0108] The foregoing description details certain embodiments of the invention. It will be appreciated, however, that no matter how detailed the foregoing appears in text, the invention can be practiced in many ways. As is also stated above, it should be noted that the use of particular terminology when describing certain features or aspects of the invention should not be taken to imply that the terminology is being re-defined herein to be restricted to including any specific characteristics of the features or aspects of the invention with which that terminology is associated. The scope of the invention should therefore be construed in accordance with the appended claims and any equivalents thereof.
EXAMPLE
Preparation of the Microorganism Solutions/Formula/Compositions
[0109] An exemplary nanozeolite was prepared by using a simple co-precipitation method. At first, sodium silicate solution (73.3 g/100 ml of distilled water) and 8.33 ml of ethylene glycol were mixed in a three-necked round bottom flask. Further, the flask was fitted with a reflux condenser and dropping funnels. Then, the mixture was stirred for 30 min with a magnetic stirrer at a temperature of 50-60° C. Next, a pre-prepared aluminum sulfate solution (26.23 g/83 ml) and sodium hydroxide solution (10 g/83 ml) were added dropwise while stirring and maintaining the same temperature. After completing the reaction, the pH level of the solution was neutralized. Later, the sample was filtered, dried at 105° C. and finally was annealed at 650° C. to obtain the desired porous grey colored zeolite.
[0110] In commercial practice, zeolite materials were purchased and grinded into nanozeolites using ball milling apparatus and sieving machines.
[0111] The beneficial organisms including bacteria and yeasts were selected in accordance with the Tables 1-5 above.
[0112] The beneficial organisms were extracted as described in Tables 1-5 above. The sample was multiplied using a Polymerase Chain Reaction (PCR) or the like, which was then separated using a Flotweg decanter centrifuges machine with a 0.2 μm filter screen. The composition (solution/formula) was prepared by impregnating a mixture of bacteria into nanozeolites. Room-temperature nanozeolites were mixed with water and the mixture of bacteria were added and the composition was stirred for 3 hours. Finally, the solution was filtered, cold-dried, or vacuum dried at the temperature less than 40° C. and blended at 12,000 rpm to get fine ground particles of composition (solution/formula) that was then stored in an airtight container to stay dry. Every kilo of the powder composition is mixed with 100 liters of water before use.
[0113] Other forms of base materials such as zeolite, maize starch, cassava starch, or starches from tubers were also used.
[0114] The mixture of base material and the selected beneficial microorganisms can be either in the form of liquid, dried powder, or gel. To obtain the liquid form, the mixture was emulsified with 10% to 20% sucrose.
[0115] The result mixture was called Biomicronest™, Micronest™, Biomicroer™, Bioenhancer™, Bioaquaer™, Biostimuler™, Biophobobionest™, and Organiqua™.
Experiments
Experiment 1: Hydrogen Sulfide (H.SUB.2.S) Reduction
[0116] Some bacteria listed in Table 1-Table 5 express strong hydrogen sulfide (H.sub.2S) reduction capabilities. In the fowl and swiftlet bird houses, the observed samples of hydrogen sulfide (H.sub.2S) were 1-10 ppm. More than 10 times the amount of H.sub.2S found in swiftlet houses (122 mg/liter) were mixed in vitro with bacteria in Table 1-Table 5. The following results were reported:
[0117] The first H.sub.2S sample in column 1 of Table 6 without any H.sub.2S reducing bacteria was used for the purpose of comparison. Those samples infested with bacteria from Tables 1-5 showed that the amount of H.sub.2S reduced over the period of 48 hours was almost 80% faster than the compared sample free of bacteria.
TABLE-US-00006 TABLE 6 H.sub.2S Reduction Capabilities of Bacteria Used in the Present Invention Remaining H.sub.2S in Samples Overtime (mg/l) No. Observed Samples 0 h 6 h 24 h 30 h 48 h 1 Sample free of Bacteria 112.sup.a ± 0.00 112.sup.e ± 0.00 80.sup.e ± 0.00 48.sup.d ± 0.00 48.sup.e ± 0.00 2 Xanthomonas spp. 144.sup.c ± 0.00 80.sup.a ± 0.00 48.sup.c ± 0.00 40.sup.c ± 0.04 48.sup.e ± 0.00 3 Xanthomonas bromi 112.sup.a ± 0.00 112.sup.e ± 0.00 48.sup.c ± 0.00 48.sup.d ± 0.00 32.sup.c ± 0.07 4 Rhothia amarae 128.sup.b ± 0.07 112.sup.e ± 0.00 48.sup.c ± 0.00 48.sup.d ± 0.00 48.sup.e ± 0.00 5 Rhodopseudomonas 112.sup.a ± 0.00 104.sup.d ± 0.04 48.sup.c ± 0.00 16.sup.a ± 0.00 16.sup.a ± 0.00 palustris 6 Paracoccus denitrificans 112.sup.a ± 0.00 88.sup.b ± 0.04 64.sup.d ± 0.07 40.sup.c ± 0.04 16.sup.a ± 0.00 7 Pseudomonas spp. 144.sup.c ± 0.00 96.sup.c ± 0.07 48.sup.c ± 0.00 32.sup.b ± 0.07 32.sup.c ± 0.07 8 Pseudomonas spp. 112.sup.a ± 0.00 112.sup.e ± 0.00 48.sup.c ± 0.00 40.sup.c ± 0.04 48.sup.e ± 0.00 9 Xanthomonas spp. 112.sup.a ± 0.00 80.sup.a ± 0.00 48.sup.c ± 0.00 48.sup.d ± 0.00 48.sup.e ± 0.00 10 Rhodococcus spp. 112.sup.a ± 0.00 96.sup.c ± 0.07 24.sup.a ± 0.04 16.sup.a ± 0.00 16.sup.a ± 0.00 11 Xanthomonas spp. 144.sup.c ± 0.00 112.sup.e ± 0.00 80.sup.e ± 0.00 48.sup.d ± 0.00 40.sup.d ± 0.04 12 Paracoccus denitrificans 112.sup.a ± 0.00 80.sup.a ± 0.00 40.sup.b ± 0.04 40.sup.c ± 0.04 24.sup.b ± 0.04
Experiment 2: Ammonia Oxidation
[0118] Some bacteria listed in Table 1-Table 5 express strong nitrifying capabilities. In the fowl and swiftlet bird houses, the amount of ammonia in guano was accumulated to toxic level that would cause swiftlets to leave their nests. The initial amount of ammonia of 10 mg/liter were mixed in vitro with bacteria in Table 1-Table 5. The following results were reported:
[0119] The first ammonia (NH.sub.4.sup.+) sample in column 1 of Table 6 without any nitrifying bacteria was used for the purpose of comparison. Those samples infested with bacteria from Tables 1-5 showed that the amount of initial ammonia reduced over the period of 48 hours was almost 90% faster than the compared sample free of nitrifying bacteria.
TABLE-US-00007 TABLE 7 TOTAL AMMONIA NITROGEN (TAN) OXIDATION CAPABILITIES Time (Hr) 0 24 48 72 96 120 Bacteria Amount of NH.sub.4.sup.+ (mg/l) Compared 10.7.sup.a ± 0.01 10.3.sup.r ± 0.01 10.2.sup.r ± 0.01 10.1.sup.q ± 0.00 9.8.sup.o ± 0.01 9.8.sup.t ± 0.01 Sample Free of Microorganisms Pseudomonas 10.2.sup.a ± 0.00 3.8.sup.cd ± 0.01 2.9.sup.efgh ± 0.00 2.2.sup.bc ± 0.00 2.0.sup.ghi ± 0.01 1.8.sup.mn ± 0.01 spp. Pantoea 9.92.sup.a ± 0.00 4.8.sup.gh ± 0.01 3.1.sup.fghi ± 0.00 2.5.sup.cd ± 0.00 1.9.sup.ghi ± 0.01 1.7.sup.l ± 0.01 agglomerans Klebsiella spp. 10.2.sup.a ± 0.01 7.0.sup.o ± 0.01 3.9.sup.jk ± 0.00 2.7.sup.ef ± 0.00 2.3.sup.hij ± 0.01 2.3.sup.p ± 0.01 Nitrosomonas 10.1.sup.a ± 0.01 4.6.sup.gh ± 0.02 4.3.sup.k ± 0.01 3.9.sup.jk ± 0.00 3.5.sup.m ± 0.01 3.3.sup.s ± 0.01 marina Nitrosomonas 10.4.sup.a ± 0.01 1.7.sup.a ± 0.01 1.2.sup.a ± 0.01 0.8.sup.a ± 0.00 0.8.sup.a ± 0.01 0.7.sup.c ± 0.01 marina Paenibacillus 10.2.sup.a ± 0.01 2.7.sup.b ± 0.02 2.2.sup.bc ± 0.01 1.7.sup.b ± 0.00 1.3.sup.bc ± 0.01 1.0.sup.de ± 0.01 pasadenensis Aeromnas sp. 10.1.sup.a ± 0.01 2.6.sup.b ± 0.02 1.9.sup.b ± 0.01 1.2.sup.b ± 0.00 1.1.sup.b ± 0.01 1.1.sup.ef ± 0.01 Pseudomonas 10.4.sup.a ± 0.01 5.5.sup.b ± 0.02 3.4.sup.hij ± 0.01 2.0.sup.b ± 0.01 1.5.sup.cd ± 0.01 1.2.sup.f ± 0.01 spp. Bacillus sp. 10.2.sup.a ± 0.02 3.6.sup.c ± 0.03 2.6.sup.cdef ± 0.01 1.0.sup.a ± 0.01 0.8.sup.a ± 0.01 0.8.sup.cd ± 0.01 Pseudomonas 10.1.sup.a ± 0.03 3.9.sup.cde ± 0.04 2.4.sup.cd ± 0.01 1.3.sup.b ± 0.02 1.1.sup.b ± 0.02 0.1.sup.a ± 0.01 spp. Paracoccus 10.1.sup.a ± 0.02 4.3.sup.ef ± 0.03 2.2.sup.bc ± 0.01 1.5.sup.b ± 0.01 1.1.sup.b ± 0.01 1.0.sup.de ± 0.01 denitrificans Kocuria 10.4.sup.a ± 0.02 5.1.sup.hij ± 0.04 3.3.sup.h ± 0.01 1.2.sup.b ± 0.02 1.0.sup.b ± 0.02 0.2.sup.a ± 0.01 rhizophila Pseudomonas 10.2.sup.a ± 0.03 2.4.sup.b ± 0.04 2.2.sup.bc ± 0.01 1.9.sup.bc ± 0.02 1.8.sup.gh ± 0.02 1.9.sup.no ± 0.01 spp. Ochrobactrum 10.1.sup.a ± 0.04 5.5.sup.jkl ± 0.06 2.6.sup.cdef ± 0.02 2.0.sup.bc ± 0.04 1.8.sup.gh ± 0.02 1.7.sup.l ± 0.01 spp. Burkholderia 10.2.sup.a ± 0.04 6.0.sup.mn ± 0.05 3.1.sup.fghi ± 0.02 2.7.sup.ef ± 0.03 2.4.sup.hi ± 0.02 2.3.sup.p ± 0.01 spp. Solibacillus 9.91.sup.a ± 0.04 4.1.sup.de ± 0.05 2.7.sup.defg ± 0.02 1.9.sup.bc ± 0.03 1.7.sup.gh ± 0.02 1.6.sup.jk ± 0.01 kalamii Proteus spp. 10.2.sup.a ± 0.01 5.0.sup.ghi ± 0.01 2.4.sup.bcde ± 0.01 1.7.sup.bc ± 0.00 1.5.sup.cd ± 0.01 1.5.sup.j ± 0.01 Paenibacillus 10.2.sup.a ± 0.01 6.7.sup.o ± 0.01 2.6.sup.cdef ± 0.01 2.2.sup.cd ± 0.00 2.0.sup.ghi ± 0.01 1.6.sup.jk ± 0.01 typhae Bacillus spp. 10.4.sup.a ± 0.01 5.3.sup.jkl ± 0.02 2.7.sup.def ± 0.01 1.9.sup.c ± 0.00 1.8.sup.gh ± 0.01 1.8.sup.lm ± 0.01 Paenibacillus 10.4.sup.a ± 0.01 6.2.sup.n ± 0.02 2.9.sup.efgh ± 0.01 2.4.sup.cd ± 0.01 2.0.sup.ghi ± 0.01 1.7.sup.l ± 0.01 aceti Paenibacillus 10.1.sup.a ± 0.02 5.7.sup.klm ± 0.04 3.6.sup.ij ± 0.01 3.2.sup.ghi ± 0.01 2.9.sup.jkl ± 0.02 2.5.sup.qr ± 0.01 glucanolyticus Paenibacillus 10.2.sup.a ± 0.02 5.8.sup.lmn ± 0.04 3.3.sup.ghi ± 0.01 2.9.sup.fg ± 0.02 2.8.sup.jkl ± 0.02 2.6.sup.q ± 0.01 lautus Paracoccus 10.9.sup.a ± 0.03 7.1.sup.o ± 0.04 4.6.sup.klm ± 0.02 3.0.sup.fg ± 0.02 2.9.sup.jkl ± 0.02 2.3.sup.p ± 0.01 denitrificans Nitrosomonas 10.1.sup.a ± 0.03 3.2.sup.cd ± 0.05 2.3.sup.cd ± 0.02 1.5.sup.b,c ± 0.02 0.9.sup.a ± 0.02 0.1.sup.a ± 0.01 spp. Nitrospira sp. 10.8.sup.a ± 0.04 6.7° ± 0.06 4.6.sup.klm ± 0.02 4.3.sup.lm ± 0.04 3.6.sup.mn ± 0.02 2.5 ± 0.01 Nitrosococcus 10.8.sup.a ± 0.04 2.8.sup.cd ± 0.06 2.2.sup.bc ± 0.02 1.6.sup.bc ± 0.04 1.4.sup.cd ± 0.02 0.7.sup.c ± 0.01 halophilus Rothia 10.9.sup.a ± 0.05 7.2° ± 0.07 5.l.sup.lmn ± 0.02 3.7.sup.hi ± 0.06 2.1.sup.hi ± 0.02 0.5.sup.b ± 0.01 endophytica Nitrobacter 10.7.sup.a ± 0.05 6.8° ± 0.07 3.3.sup.ghi ± 0.02 2.1.sup.cd ± 0.06 1.9.sup.ghi ± 0.02 1.4.sup.ij ± 0.01 winogradskyi Nitrosomonas 10.6.sup.a ± 0.05 4.0.sup.cde ± 0.08 3.7.sup.ij ± 0.02 2.8.sup.fg ± 0.08 1.9.sup.ghi ± 0.03 1.6.sup.jk ± 0.01 spp. Paenibacillus 10.9.sup.a ± 0.06 5.9.sup.mn ± 0.09 2.3.sup.cd ± 0.03 2.1.sup.cd ± 0.1 1.3.sup.bc ± 0.03 0.6.sup.bc ± 0.01 durus Nitrobacter 10.4.sup.a ± 0.07 8.5.sup.p ± 0.11 5.6.sup.mno ± 0.03 2.6.sup.ef ± 0.14 1.5.sup.bc ± 0.03 1.3.sup.i ± 0.01 spp. Paenibacillus 9.83.sup.a ± 0.07 5.6.sup.kl ± 0.11 4.3.sup.kl ± 0.03 3.1.sup.ghi ± 0.14 2.4.sup.hij ± 0.03 1.2.sup.f ± 0.01 favisporus Paenibacillus 10.1.sup.a ± 0.08 7.8.sup.op ± 0.11 6.2.sup.op ± 0.03 5.4.sup.mn ± 0.16 3.9.sup.op ± 0.04 2.7.sup.q ± 0.01 endophyticus Paenibacillus 9.79.sup.a ± 0.02 8.4.sup.p ± 0.02 7.5.sup.pq ± 0.01 6.3.sup.no ± 0.01 2.5.sup.ij ± 0.01 0.9.sup.d ± 0.01 glycanilyticus Nitrobacter 9.87.sup.a ± 0.03 7.6.sup.op ± 0.04 5.3.sup.lmn ± 0.02 3.6.sup.hij ± 0.02 2.9.sup.jkl ± 0.02 1.2.sup.f ± 0.01 winogradskyi Paenibacillus 10.3.sup.a ± 0.06 9.2.sup.q ± 0.09 8.6.sup.qr ± 0.03 6.5.sup.op ± 0.09 3.1.sup.kl ± 0.03 1.6.sup.jk ± 0.01 pectinilyticus
Experiment 3: Feces Decomposition Bioactivities of Beneficial Microorganisms
[0120] The beneficial microorganisms listed in Tables 1-5 above were tested for their feces decomposing bioactivities. Animal fecal samples taken from animal farms contain casein, carbohydrates, lipid, and cellulose. These samples were mixed with the beneficial microorganisms listed in Tables 1-5 above and the results were recorded in Tables 8-11 below.
TABLE-US-00008 TABLE 8 Casein Decomposing Capability of Beneficial Microorganisms The Radii of Bioactivities Microorganisms Decomposition(cm).sub.TB (U/ml) Bacillus megaterium 1.5.sup.b ± 0.02 14.13.sup.c ± 0.01 Aeromonas veronii 2.2.sup.d ± 0.03 22.5.sup.e ± 0.03 Fictibacillus 1.3.sup.a ± 0.01 8.54.sup.a ± 0.01 phosphorivorans Vogesella amnigena 1.2.sup.a ± 0.02 7.15.sup.a ± 0.02 Bacillus xiamenensis 1.3.sup.a ± 0.02 9.19.sup.b ± 0.01 Paenibacillus humicus 2.8.sup.e ± 0.03 24.97 ± 0.03 Jeotgalibacillus spp. 1.7.sup.c ± 0.01 14.02.sup.c ± 0.03 Pseudomonas spp. 1.7.sup.c ± 0.02 11.34.sup.bc ± 0.01 Lysinibacillus spp. 2.3.sup.d ± 0.03 21.96.sup.d ± 0.03
TABLE-US-00009 TABLE 9 The Radii of Carbohydrates Decomposition of the Beneficial Organisms The Radii of Bioactivities Microorganisms Decomposition(cm).sub.TB (U/ml) Bacillus pumilus 0.5.sup.c ± 0.02 5.1.sup.d ± 0.01 Bacillus amyloliquefaciens 3.6.sup.i ± 0.02 32.1.sup.i ± 0.03 Paenibacillus amylolyticus 0.7.sup.d ± 0.01 7.1.sup.e ± 0.03 Fictibacillus spp. 1.7.sup.f ± 0.02 14.8.sup.h ± 0.01 Aeromonas jandaei 0.3.sup.b ± 0.01 2.7.sup.c ± 0.03 Bacillus xiamenensis 0.8.sup.d ± 0.03 7.0.sup.e ± 0.02 Paenibacillus catalpae 0.9.sup.e ± 0.02 8.1.sup.f ± 0.01 Streptomyces sp. 0.9.sup.e ± 0.01 8.2.sup.f ± 0.02 Brevibacillus parabrevis 0.6.sup.c ± 0.02 6.0.sup.d ± 0.01 Bacillus spp. 0.9.sup.e ± 0.02 7.9.sup.e ± 0.02 Bacillus spp. 2.8.sup.h ± 0.03 13.2.sup.gh ± 0.01 Bacillus spp. 2.3.sup.g ± 0.02 12.1.sup.g ± 0.03 Bacillus sp. 0.6.sup.c ± 0.01 6.1.sup.d ± 0.01 Bacillus alritudinis 0.2.sup.b ± 0.03 1.7.sup.b ± 0.03 Bacillus velezensis 0.sup.a ± 0.02 1.1.sup.a ± 0.01 Bacillus spp. 0.3.sup.b ± 0.02 2.6.sup.c ± 0.02 Bacillus spp. 1.0.sup.e ± 0.01 11.2.sup.g ± 0.01
TABLE-US-00010 TABLE 10 Cellulase Bioactivities of Beneficial Microorganisms The Radii of Bioactivities Microorganisms Decomposition(cm).sub.TB (U/ml) Paenibacillus odorifer 2.1.sup.i ± 0.12 24.4.sup.i ± 0.35 Bacillus spp. 5.0.sup.k ± 0.21 32.1.sup.k ± 0.57 Paenibacillus catalpae 1.8.sup.f ± 0.15 19.4.sup.g ± 0.28 Paenibacillus 1.8.sup.f ± 0.14 22.5.sup.h ± 0.25 xylanexedens Bacillus stratosphericus 0.5.sup.a ± 0.16 8.1.sup.a ± 0.36 Paenibacillus 1.4.sup.d ± 0.35 16.3.sup.ef ± 0.64 cellulosilyticus Bacillus xiamenensis 1.4.sup.d ± 0.23 18.1.sup.fg ± 0.24 Acidovorax spp. 1.6.sup.e ± 0.32 13.2.sup.d ± 0.24 Fictibacillus 1.0.sup.ab ± 0.46 9.2.sup.a ± 0.24 phosphorivorans Bacillus aryabhattai 2.3.sup.j ± 0.23 22.1.sup.h ± 0.35 Bacillus spp. 1.4.sup.d ± 0.24 19.3.sup.g ± 0.27 Lysinibacillus spp. 2.4.sup.j ± 0.42 30.1.sup.j ± 0.48 Pantoea eucalypti 5.5.sup.l ± 0.43 47.3.sup.l ± 0.36 Paenibacillus 1.1.sup.b ± 0.24 9.2.sup.a ± 0.35 xylanexedens Bacillus spp. 1.9.sup.f ± 0.46 21.3.sup.h ± 0.25 Paenibacillus 1.2.sup.c ± 0.25 10.3.sup.c ± 0.53 xylanilyticus Stenotrophomonas 0.9.sup.a ± 0.37 8.5.sup.a ± 0.73 maltophilia Paenibacillus 1.3.sup.cd ± 0.24 15.1.sup.e ± 0.46 cellulositrophicus Paenibacillus mendelii 2.2.sup.i ± 0.25 19.2.sup.g ± 0.25 Paenibacillus 1.6.sup.e ± 0.23 17.1.sup.f ± 0.56 chitinolyticus Paenibacillus 2.2.sup.i ± 0.24 24.2.sup.i ± 0.56 pasadenensis
TABLE-US-00011 TABLE 11 Lipase Bioactivities of Beneficial Microorganisms The Radii of Bioactivities Microorganisms Decomposition(cm).sub.TB (U/ml) Paenibacillus catalpae 1.7.sup.e ± 0.46 11.43.sup.c ± 0.67 Bacillus stratosphericus 0.9.sup.a ± 0.32 5.76.sup.a ± 0.56 Xanthomonas spp. 2.2.sup.f ± 0.54 12.32c ± 0.72 Lysinibacillus fusiformis 2.6.sup.f ± 0.42 13.34.sup.d ± 0.45 Bacillus altitudinis 1.3.sup.c ± 0.23 6.13.sup.a ± 0.43 Paenibacillus polymyxa 2.9.sup.h ± 0.26 23.34.sup.h ± 0.43 Paenibacillus typhae 2.1.sup.ef ± 0.24 15.54.sup.e ± 0.67 Bacillus spp. 2.6.sup.f ± 0.45 13.32.sup.d ± 0.25 Paenibacillus 1.6.sup.d ± 0.23 15.75.sup.e ± 0.54 antibioticophila Paenibacillus 1.2.sup.b ± 0.15 13.32.sup.d ± 0.65 pasadenensis Geobacillus zalihae 1.1.sup.b ± 0.67 13.41.sup.d ± 0.34 Brevibacillus para brevis 1.2.sup.b ± 0.33 9.65.sup.b ± 0.54 Burkholderia cepacia 1.4.sup.c ± 0.65 6.23.sup.a ± 0.12 Bacillus spp. 1.6.sup.d ± 0.21 13.58.sup.d ± 0.54 Cytobacillus firmus 1.7.sup.e ± 0.21 16.53.sup.ef ± 0.24 Paenibacillus spp. 1.5.sup.cd ± 0.24 11.23c ± 0.55 Pseudomonas spp. 2.8.sup.g ± 0.21 21.64.sup.g ± 0.35 Paenibacillus herberti 1.6.sup.d ± 0.01 11.14.sup.c ± 0.24 Geobacillus 2.5.sup.ef ± 0.32 21.14.sup.g ± 0.25 thermocatenulatus Pseudomonas spp. 1.4.sup.c ± 0.21 17.54.sup.f ± 0.56 Bacillus spp. 1.6.sup.d ± 0.34 11.43.sup.c ± 0.32
Experiment 4
[0121] 500 swiftlet bird houses in Binh Thuan province, Bac Lieu City, and other areas near Ho Chi Minh City in Vietnam were tested with the beneficial microorganism described above. These swiftlet bird houses were either brand new or existing swiftlet bird houses. The test results showed a 100% improvement in these swiftlet bird houses. In the new bird houses, swiftlets were attracted and came in to nest in less than two hours. In the old bird houses, the odors were eliminated. Later on, the bird nests showed a brighter color than the old ones. Other results were also observed and listed below:
[0122] (a) Ammonia in the swiftlets' feces was oxidized and transformed to non-toxic organic nitrogen (N.sub.2);
[0123] (b) Hydrogen sulfide (H.sub.2S) was eliminated;
[0124] (c) Acrimonious odors from the swiftlets' guano were substantially reduced, improving the air quality essential to the nesting and the high-quality of the edible bird's nests (EBN);
[0125] (d) The swiftlets paired up and nested more than usual; and
[0126] (e) The bird's nests harvested had a healthy opaque color.
DESCRIPTION OF NUMERALS
[0127] 400 Swiftlet bird house
[0128] 401 Ground floor
[0129] 402 First Floor
[0130] 403 Second floor
[0131] 404 Roof
[0132] 405 Footing and Foundation
[0133] 406 Ground floor removable rug for containing guano
[0134] 407 First floor removable rug
[0135] 408 Second floor (top floor) removable rug
[0136] 409 Exit/entrance window
[0137] 411 Computer and speaker box
[0138] 412 Speakers
[0139] 421 Sensors