Chemoenzymatic synthesis of trehalose analogs

Abstract

The present invention provides methods of synthesizing trehalose analogues; methods of detecting mycobacteria, and trehalose analogues.

Claims

1. A compound according to formula (III) or (IV) ##STR00010## wherein X is S, Se, PH, P(O)H, or P(O)OH; R.sup.1, R.sup.2 and R.sup.3 are independently H, F, OR, SR, SCOR, OSO.sub.3.sup., SeR, SeCOR, N.sub.3, CN, NC, C.sub.1-6 alkyl, C.sub.2-6 alkenyl, or C.sub.2-6 alkynyl; R.sup.4 is OH, NHR, SH, or SeH; and each R is independently H, C.sub.1-6 alkyl, C.sub.2-6 alkenyl or C.sub.2-6 alkynyl.

2. The compound according to claim 1 that is ##STR00011##

3. The compound according to claim 1, wherein the compound has formula (III).

4. The compound according to claim 1, wherein the compound has formula (IV).

5. The compound according to claim 1, wherein X is S.

6. The compound according to claim 5, wherein the compound has formula (III).

7. The compound according to claim 5, wherein the compound has formula (IV).

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) FIG. 1A, FIG. 1B, FIG. 1C, and FIG. 1D show the synthesis of trehalose analogues from Glc analogues and UDP-Glc by TreT from T. tenax. In FIG. 1A, FIG. 1B, FIG. 1C, and FIG. 1D yields were determined by HPLC and N.D. indicates not detected.

(2) FIG. 2A and FIG. 2B. FIG. 2A shows the experimental workflow for single-day probe synthesis, metabolic labeling, and imaging of M. smegmatis, and in FIG. 2B fluorescence microscopy analysis of 6-TreAz-treated (or untreated) M. smegmatis wild type, sugC mutant, and sugC::sugC complement, indicating that trehalose analogue probes can accumulate in mycobacteria via the trehalose-specific transporter SugABC-LpqY. TL, transmitted light. Scale bars, 5 m.

(3) FIG. 3A, FIG. 3B, FIG. 3C, FIG. 3D, and FIG. 3E. FIG. 3A shows the experimental workflow for assessing uptake of fluoro-trehalose analogues by M. smegmatis and in FIG. 3B, FIG. 3C, FIG. 3D, and FIG. 3E gas chromatograms for analysis of fluoro-trehalose analogue uptake in M. smegmatis wild type, sugC mutant, and sugC::sugC complement, indicating that trehalose analogue probes can accumulate in mycobacteria via the trehalose-specific transporter SugABC-LpqY.

(4) FIG. 4 shows the one-pot metabolic labeling.

DETAILED DESCRIPTION

(5) Before any embodiments of the invention are explained in detail, it is to be understood that the invention is not limited in its application to the details of construction and the arrangement of components set forth in the following description or illustrated in the following drawings. The invention is capable of other embodiments and of being practiced or of being carried out in various ways.

(6) The present invention provides chemoenzymatic methods for synthesizing trehalose analogues using a trehalose synthase (TreT) enzyme. In nature, TreT converts glucose and UDP-glucose into trehalose in a single step. The present invention uses analogues of glucose and/or UDP-glucose in the TreT reaction to generate trehalose analogues. It is expected that the methods of the present invention will be useful for rapidly and efficiently preparing trehalose analogues as potential anti-mycobacterial compounds, compounds for imaging/detection of mycobacteria, and non-degradable biopreservation/bioprotection compounds, among other applications in the biological and materials sciences. For example, these methods could be used for transforming easily accessible radiolabeled glucose analogues, e.g. [.sup.14C]-labeled glucose or [.sup.18F]-labeled glucose, into the corresponding [.sup.14C]- or [.sup.18F]-labeled trehalose analogues for investigating or imaging mycobacteria and other trehalose-containing organisms. These types of trehalose analogues are virtually inaccessible using current synthesis methods or are exceedingly expensive to purchase. The methods of the present invention will provide a simple, fast (1 hour), and high-yielding (up to 99% yield) route to synthesizing trehalose analogues for various applications.

(7) Definitions

(8) As used herein, the term alkyl refers to a branched, unbranched, or cyclic hydrocarbon having, for example, from 1 to 6 carbon atoms or from 1 to 4 carbon atoms. Examples include, but are not limited to, methyl, ethyl, 1-propyl, 2-propyl, 1-butyl, 2-methyl-1-propyl, 2-butyl, 2-methyl-2-propyl (t-butyl), 1-pentyl, 2-pentyl, 3-pentyl, 2-methyl-2-butyl, 3-methyl-2-butyl, 3-methyl-1-butyl, 2-methyl-1-butyl, 1-hexyl, 2-hexyl, 3-hexyl, 2-methyl-2-pentyl, 3-methyl-2-pentyl, 4-methyl-2-pentyl, 3-methyl-3-pentyl, 2-methyl-3-pentyl, 2,3-dimethyl-2-butyl, 3,3-dimethyl-2-butyl, and the like. The alkyl can be unsubstituted or substituted. The alkyl can also be optionally partially or fully unsaturated. As such, the recitation of an alkyl group includes both alkenyl and alkynyl groups. The alkyl can be a monovalent hydrocarbon radical, as described and exemplified above, or it can be a divalent hydrocarbon radical (i.e., alkylene).

(9) The term alkenyl refers to a monovalent branched or unbranched partially unsaturated hydrocarbon chain (i.e. a carbon-carbon, sp.sup.2 double bond). In some embodiments, an alkenyl group can have from 2 to 6 carbon atoms, or 2 to 4 carbon atoms. Examples include, but are not limited to, ethylene or vinyl, allyl, cyclopentenyl, 5-hexenyl, and the like. The alkenyl can be unsubstituted or substituted.

(10) The term alkynyl refers to a monovalent branched or unbranched hydrocarbon chain, having a point of complete unsaturation (i.e. a carbon-carbon, sp triple bond). In some embodiments, the alkynyl group can have from 2 to 6 carbon atoms, or 2 to 4 carbon atoms. This term is exemplified by groups such as ethynyl, 1-propynyl, 2-propynyl, 1-butynyl, 2-butynyl, 3-butynyl, 1-hexynyl, 2-hexynyl, 3-hexynyl, and the like. The alkynyl can be unsubstituted or substituted.

(11) The term halo refers to fluoro, chloro, bromo, and iodo. Similarly, the term halogen refers to fluorine, chlorine, bromine, and iodine.

(12) As used herein, the term substituted is intended to indicate that one or more (e.g., 1, 2, 3, 4, or 5; in some embodiments 1, 2, or 3; and in other embodiments 1 or 2) hydrogens on the group indicated in the expression using substituted is replaced with a selection from the indicated group(s), or with a suitable group known to those of skill in the art, provided that the indicated atom's normal valency is not exceeded and that the substitution results in a stable compound. Suitable indicated groups include, e.g., alkoxy, oxo, halo, haloalkyl, hydroxy, hydroxyalkyl, amino, alkylamino, dialkylamino, nitro, and cyano. Additionally, the suitable indicated groups can include, e.g., X, R, O.sup., OR, SR, S.sup., NR.sub.2, NR.sub.3, NR, CX.sub.3, CN, OCN, SCN, NCO, NCS, NO, NO.sub.2, N.sub.2, N.sub.3, C(O)R, C(O)X, C(O)OR, where each X is independently a halogen (halo): F, Cl, Br, or I; and each R is independently H or alkyl. As would be readily understood by one skilled in the art, when a substituent is oxo (O), or the like, then two hydrogen atoms on the substituted atom are replaced.

(13) Synthesis

(14) In some embodiments, the present invention provides a method for synthesizing trehalose analogues comprising contacting a glucose analogue with a trehalose synthase, a magnesium salt, and a monosaccharide donor. In some embodiments, the present invention provides trehalose analogues quickly, e.g. in about 1 hour, in a high yield, e.g. up to 99%, in a single step from readily available glucose analogues.

(15) ##STR00002##

(16) In some embodiments, the monosaccharide donor is a nucleotide diphosphate monosaccharide, such as UDP-glucose, ADP-glucose, GDP-glucose, UDP-galactose, or GDP-mannose. For example, the nucleotide diphosphate may be uridine diphosphate (UDP), guanosine diphosphate (GDP), or adenosine diphosphate (ADP) and the monosaccharide may be glucose, galactose, allose, or mannose. In some embodiments, the monosaccharide may be labeled, e.g. with a radioactive or other isotope, such as .sup.11C, .sup.13C, .sup.14C, .sup.2H, .sup.3H, .sup.15O, .sup.18O, .sup.13N, .sup.15N, .sup.35S, .sup.18F and .sup.125I. In other embodiments, the monosaccharide donor is glucosyl fluoride.

(17) The magnesium salt is suitably magnesium chloride. The reaction may further comprise a buffer, such as HEPES, Tris, or other common biological buffers. In some embodiments, the reaction is performed at a pH of about 7 to about 7.5.

(18) In some embodiments, the methods of the present invention may provide a yield of at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 97%, at least about 98%, or at least about 99%. In some embodiments, the methods of the present invention may be performed in less than about 1 hour, less than about 2 hours, less than about 3 hours, less than about 6 hours, less than about 12 hours, or less than about 24 hours.

(19) In some embodiments, the trehalose synthase is thermostable. In some embodiments, the trehalose synthase is unidirectional, meaning that it does not degrade trehalose. In some embodiments, the trehalose synthase is from Thermoproteus tenax. Suitable trehalose synthases include, but are not limited to, those in Thermoccus litoralis, Pyrococcus horikoshii, Thermoproteus tenax, Rubrobacter xylanphilus, Pimelobacter sp., Thermus aquaticus, Sulfolobus sp. The trehalose synthase may be isolated and purified from E. coli by techniques known to one of ordinary skill in the art.

(20) In some embodiments, the glucose analogue is a monosaccharide. The analogue may be a natural sugar, such as mannose, galactose, or allose, or a non-natural sugar, such as a modified glucose, modified mannose, modified galactose, or a modified allose. In some embodiments, the glucose analogue is modified at the 2-, 3-, 4- and/or 6-positions. In some embodiments, the glucose analogue may contain stereochemical modifications. Certain glucose analogues suitable for use in the present invention contain both substituent modifications and stereochemical modifications. In some embodiments, the glucose analogue contains one or more of the following modifications: a stereochemical modification; a halo substituent, such as fluoro or chloro; a deoxy modification; an azido substituent; a hydroxyl substituent; a ring sulfur; an isotope.

(21) In some embodiments, the glucose analogue may contain one or more isotopes, such as, but not limited to, .sup.11C, .sup.13C, .sup.14C, .sup.2H, .sup.3H, .sup.15O, .sup.18O, .sup.13N, .sup.15N, .sup.35S, .sup.18F and .sup.125I. In some embodiments, one or more of the isotopes may be a radioactive isotope. In some embodiments, an isotope may be located at any position in the glucose analogue. For example, the ring may contain an isotope. In some embodiments, an isotope may be found in one or more of R.sup.1, R.sup.2, R.sup.3 and R.sup.4. In some embodiments, an isotope may be located in the ring and in one or more of R.sup.1, R.sup.2, R.sup.3 and R.sup.4.

(22) In some embodiments, the glucose analog may be further substituted by a detectable moiety that is detectable upon exposure to an external stimulus. In some embodiments, the detectable moiety may be a reactive chemical handle (e.g. a biorthoganol tag such as azido, alkynyl, or cylcopropenyl), a fluorophore, a luminescent moiety (e.g. a chemiluminescent moiety, a thermoluminescent moiety, or an electroluminescent moiety), or a phosphorescent moiety. Suitable fluorophores include, but are not limited to, fluoresceins, xanthenes, cyanines, naphthalenes, coumarins, oxadiazoles, pyrenes, oxazines, acridines, arylmethines, Alexa Fluors and tetrapyrroles. In some embodiments, the detectable moiety is attached to the glucose analogue through a reactive chemical moiety such as azido, alkynyl or cyclopropenyl.

(23) The glucose analogue may suitably be a compound of Formula (I):

(24) ##STR00003##
wherein

(25) X is O, S, Se, PH, P(O)H, or P(O)OH;

(26) R.sup.1, R.sup.2 and R.sup.3 are independently selected from H, halo, OR, NR.sub.2, NR.sub.3.sup.+, NHCOR, SR, SCOR, OPO.sub.3.sup.2, SeR, SeCOR, N.sub.3, CN, NC, C.sub.1-6 alkyl, C.sub.2-6 alkenyl, or C.sub.2-6 alkynyl;

(27) R.sup.4 is OH, NHR, SH, or SeH; and

(28) each R is independently selected from H, C.sub.1-6 alkyl, C.sub.2-6 alkenyl or C.sub.2-6 alkynyl.

(29) The glucose analogue may suitably be a compound of Formula (II):

(30) ##STR00004##
wherein

(31) X is O, S, Se, PH, P(O)H, or P(O)OH;

(32) R.sup.1, R.sup.2 and R.sup.3 are independently selected from H, halo, OR, NR.sub.2, NR.sub.3.sup.+, NHCOR, SR, SCOR, OPO.sub.3.sup.2, SeR, SeCOR, N.sub.3, CN, NC, C.sub.1-6 alkyl, C.sub.2-6 alkenyl, or C.sub.2-6 alkynyl;

(33) R.sup.4 is OH, NHR, SH, or SeH; and

(34) each R is independently selected from H, C.sub.1-6 alkyl, C.sub.2-6 alkenyl or C.sub.2-6 alkynyl.

(35) As described herein, all glucose and trehalose analogues include all possible isotopic substitutions. For example, H may be in any isotopic form, including .sup.1H, .sup.2H (D), and .sup.3H (T); C may be in any isotopic form, including .sup.12C, .sup.13C, and .sup.14C; O may be in any isotopic form, including .sup.16O and .sup.18O; and the like.

(36) Exemplary substrates and their products are shown in FIGS. 1A-D.

(37) Use of Trehalose Analogues

(38) The present invention also provides a simple method for the synthesis and use of chemical probes to detect live mycobacteria, e.g., Mycobacterium tuberculosis, and possibly other trehalose-containing organisms/pathogens. In some embodiments, this method is performed without purification of the enzymatic reaction. This is referred to as one-pot metabolic labeling. One-pot metabolic labeling capitalizes on the biocompatible nature of enzymatic reactions. After performing the enzymatic reaction as described above, the reaction mixture containing the trehalose analogue product can be directly added to a sample containing live mycobacteria (or possibly other trehalose-containing organisms/pathogens) with no or minimal processing or purification of the enzymatic reaction (as shown in Scheme 2). The mycobacteria (or possibly other trehalose-containing organisms/pathogens) take up the trehalose analogue from the aqueous enzymatic reaction mixture, leading to labeling of the cell. If the trehalose analogue is detectable (e.g., .sup.14C-, .sup.18F-, .sup.3H, or N.sub.3-labeled or labeled with a reactive chemical handle, a fluorophore, a luminescent moiety or a phosphorescent moiety), then mycobacteria (or possibly other trehalose-containing organisms/pathogens) are readily detected using traditional analytical methods known to one skilled in the art, such as positron emission topography (PET), autoradiographic analysis, scintillation detection, magnetic resonance imaging (MRI), nuclear magnetic resonance (NMR), x-ray photography, computed tomography (CT), single photon emission computed tomography (SPECT) and fluorescence microscopy. In some embodiments, one-pot metabolic labeling provides trehalose analogue synthesis, metabolic labeling, and imaging of mycobacteria in about 1 hour, or about 2 hours or about 3 hours or about 5 hours or about 10 hours. This one-pot approach is useful for rapidly synthesizing and administering trehalose analogues to mycobacteria (or possibly other trehalose-containing organisms/pathogens). See FIG. 4.

(39) The present invention also provides a method of determining the presence of mycobacteria species in a subject or sample. In some embodiments, the method comprises adding a labeled trehalose analogue to an organism or sample. The presence of mycobacteria is then determined by detecting the labeled trehalose analogue. Without wishing to be bound by theory, the labeled trehalose analogue accumulates in mycobacteria via a trehalose-specific transport protein, such as SugABC-LpqY. In some embodiments, the trehalose analogue is labeled with an isotope, such as .sup.18F. In some embodiments, the trehalose analog is labeled with a detectable moiety such as a fluorophore, a luminescent moiety or a phosphorescent moiety.

(40) The methods of the present invention are useful in cells grown in culture medium, i.e., in vitro, or in cells within animals, e.g., living animals, i.e., in vivo. For research purposes, for measurements in cells in vivo, a trehalose analogue as described herein is administered, e.g., injected into the animal or added to an aqueous solution, e.g., water, or food consumed by the animal, to the animal. The methods of the present invention may be used in intact cells or in isolated organelles.

(41) Cells may be mammalian cells, including but not limited to human, simian, murine, canine, bovine, equine, feline, ovine, caprine or swine cells, or prokaryotic cells, or cells from two or more different organisms, or cell lysates or supernatants thereof. In certain aspects, the cell may be in an animal, or physiological fluid, e.g., blood, plasma, urine, mucous secretions or the like.

(42) In some embodiments, the sample is a biological sample such as tissue, cells, bodily fluid, sputum, cerebrospinal fluid, pericardial fluid, synovial fluid, ascitic fluid, blood, bone marrow, urine, or feces.

(43) Trehalose Analogues

(44) The present application also provides various trehalose analogues. In some embodiments, the trehalose analogue is a compound according to Formula (III) or Formula (IV):

(45) ##STR00005##
wherein

(46) X is O, S, Se, PH, P(O)H, or P(O)OH;

(47) R.sup.1, R.sup.2 and R.sup.3 are independently selected from H, halo, OR, NR.sub.2, NR.sub.3.sup.+, NHCOR, SR, SCOR, OPO.sub.3.sup.2, SeR, SeCOR, N.sub.3, CN, NC, C.sub.1-6 alkyl, C.sub.2-6 alkenyl, or C.sub.2-6 alkynyl;

(48) R.sup.4 is OH, NHR, SH, or SeH; and

(49) each R is independently selected from H, C.sub.1-6 alkyl, C.sub.2-6 alkenyl or C.sub.2-6 alkynyl;

(50) with the proviso that the analogue is not trehalose.

(51) Trehalose analogues contemplated by the present invention include, but are not limited to:

(52) ##STR00006## ##STR00007## ##STR00008##

(53) It is specifically understood that any numerical value recited herein (e.g., ranges) includes all values from the lower value to the upper value, i.e., all possible combinations of numerical values between the lowest value and the highest value enumerated are to be considered to be expressly stated in this application. For example, if a concentration range is stated as 1% to 50%, it is intended that values such as 2% to 40%, 10% to 30%, or 1% to 3%, etc., are expressly enumerated in this specification. These are only examples of what is specifically intended.

EXAMPLES

Example 1: Materials and Methods for Examples 2 and 3

(54) General Experimental Section: Materials and reagents were obtained from commercial sources without further purification. Most glucose analogues were obtained from Sigma-Aldrich (2-GlcAz, 2-DeoxyGlc, 6-GlcAz), CarboSynth (2-FluoroGlc, 3-DeoxyGlc, 3-FluoroGlc, 4-DeoxyGlc, 4-FluoroGlc, 6-DeoxyGlc, 6-FluoroGlc), or Santa Cruz Biotechnology (5-ThioGlc). 3-GlcAz and 4-GlcAz were chemically synthesized according to literature-reported procedures. Chromatographic analysis of reactions was performed using a Perkin Elmer Series 200 high-performance liquid chromatography (HPLC) system equipped with a Perkin Elmer Series 200 refractive index detector. High-resolution electrospray ionization (HR ESI) mass spectra were obtained in negative mode using a Waters LCT Premier XE using raffinose as the lock mass. .sup.1H NMR and .sup.13C NMR spectra were recorded on a Varian Inova 500 MHz NMR. Analytical TLC was performed on glass-backed silica gel 60 plates (thickness 250 m) from Dynamic Adsorbents and detected by charring with 5% H.sub.2SO.sub.4 in EtOH. Column chromatography was performed using flash-grade silica gel 32-63 m (230-400 mesh) from Dynamic Adsorbents.

(55) Cloning, Expression, and Purification of TreT:

(56) The treT gene encoding trehalose synthase from Thermoproteus tenax was codon-optimized for E. coli and synthesized by Life Technologies. The optimized treT sequence was cloned into the pBAD/His A vector using restriction sites KpnI and SacI on the N-terminus and C-terminus, respectively, and verified by sequencing. The resultant pBAD-HisA TreT plasmid encodes an N-terminal 6histidine tag on the protein. The plasmid was transformed into Top10 chemically competent E. coli and plated on LB agar containing 100 m/mL ampicillin.

(57) To express the TreT protein, a 3 mL LB/ampicillin culture was inoculated with a single colony and grown overnight in a shaking incubator at 37 C. The following day, this 3 mL culture was used to inoculate a 600 mL culture of Terrific Broth with 100 m/mL ampicillin. Once the inoculated culture reached mid-log phase, it was induced with arabinose (final concentration of arabinose=1 mM) and grown in a shaking incubator overnight at 37 C.

(58) Cells were then spun at 4000g and the pellets resuspended in 20 mL of Equilibration/Lysis/Wash buffer (300 mM NaCl, 50 mM NaH.sub.2PO.sub.4, 20 mM imidazole). Resuspended pellets were sonicated using a Fisher Scientific Sonifier (345 s, 75% amplitude). Sonicated cells were spun at 15,000g for 20 minutes to clarify the lysate. Next, lysates were loaded on a 5 mL Bio-Rad Bio-scale Mini Profinity immobilized metal affinity chromatography (IMAC) cartridge at 5 mL/minute and washed until the A.sub.280 reached background levels. TreT protein was eluted using a gradient containing high imidazole buffer (300 mM NaCl, 50 mM NaH.sub.2PO.sub.4, 250 mM imidazole). Protein samples were concentrated in an Amicon Ultra 15 mL concentrator with a 10,000 molecular weight cutoff. Elution buffer was exchanged for 300 mM NaCl, 50 mM HEPES, pH 7. Protein samples were analyzed by SDS-PAGE to verify protein purity and concentration was assessed using UV-Vis spectroscopy.

(59) General Method for Enzymatic Reactions:

(60) Concentrated (10) stock solutions of the reaction reagents in 50 mM HEPES buffer (pH 7.4) included glucose analogues (100 mM), UDP-Glc (400 mM), and MgCl.sub.2 (200 mM). Microscale reactions (50 L) were performed by addition of 5 L of each reagent and an appropriate volume of HEPES buffer, followed by addition of TreT to a final concentration of 9.8 M to initiate the reaction. Reactions were incubated at 70 C. with gentle shaking for 1 h, after which an equal volume of ice-cold HPLC-grade acetone (50 L) was added to quench the reaction. After cooling at 20 C. for 1 h, reactions were centrifuged at 11,900 rpm for 20 min, and the supernatant was collected and directly analyzed by HPLC (column: Imtakt UK-Amino 25046 mm at 50 C.; mobile phase: isocratic elution with pre-mixed 80% acetonitrile in water; flow rate: 0.4 mL/min; detection: refractive index). Yields were calculated by comparing relative peak areas of substrates and products.

(61) .sup.1H NMR, .sup.13C NMR, and HR ESI MS Data for Semi-Preparative Reaction Products:

(62) Semi-preparative reactions were performed exactly as described above only in larger final volumes (1.5-2.0 mL). After incubation for 1 h at 70 C., reactions were quenched by addition of an equal volume of cold acetone, cooled at 20 C. for 1 h, and centrifuged at 11,900 rpm for 20 min. The supernatant was collected and concentrated in vacuum, and the resulting residue was resuspended in CH.sub.2Cl.sub.2/CH.sub.3OH (1:1) and passed through a silica plug using CH.sub.2Cl.sub.2/CH.sub.3OH (1:1) as the eluent. If necessary, further purification was accomplished by silica gel column chromatography using an elution solvent of CH.sub.2Cl.sub.2/CH.sub.3OH (2:1 or 2.5:1). After concentration, the purified products were redissolved in water, filtered (0.2 m), and concentrated to give white solids.

2-Deoxy-2-fluoro-,-D-trehalose (2-FluoroTre)

(63) From 3.6 mg 2-FluoroGlc, obtained 6.6 mg 2-FluoroTre (97%). .sup.1H NMR (500 MHz, D.sub.2O): 5.41 (d, J=3.5 Hz, 1H), 5.19 (d, J=3.5 Hz, 1H), 4.48 (ddd, J.sub.H,H=3.5, 9.5 Hz, J.sub.H,F=49 Hz, 1H), 4.10 (dt, J.sub.H,H=9.5 Hz, J.sub.H,F=13.5 Hz, 1H), 3.88-3.72 (m, 7H), 3.63 (dd, J=3.5, 10 Hz, 1H), 3.49 (t, J=9.0 Hz, 1H), 3.43 (t, J=10 Hz, 1H). .sup.13C NMR (125 MHz, D.sub.2O): 93.97, 91.15 (d, J.sub.C,F=22 Hz), 89.44 (d, J.sub.C,F=188 Hz), 72.54, 72.16, 71.05 (d, J.sub.C,F=17 Hz), 70.89, 69.51, 69.02, 68.96, 60.43, 60.22. HR ESI MS negative mode: calcd. for C.sub.12H.sub.21ClFO.sub.10 [M+Cl].sup. m/z, 379.0807; found, 379.0800.

2-Deoxy-,-D-trehalose (2-DeoxyTre)

(64) From 3.3 mg of 2-DeoxyGlc, obtained 6.2 mg 2-DeoxyTre (94%). .sup.1H NMR (500 MHz, D.sub.2O): 5.28 (d, J=3.0 Hz, 1H), 5.13 (d, J=3.5 Hz, 1H), 4.03 (m, 1H), 3.86-3.72 (m, 6H), 3.67 (m, 1H), 3.59 (dd, J=3.5, 9.5 Hz, 1H), 3.42 (t, J=9.0 Hz, 1H), 3.38 (t, J=9.0 Hz, 1H), 2.20 (dd, J=5.0, 13 Hz, 1H), 1.75 (dt, J=3.5, 13 Hz). .sup.13C NMR (125 MHz, D.sub.2O): 93.19, 92.30, 72.64, 72.61, 72.12, 70.90, 69.59, 67.88, 60.57, 60.43, 36.27. HR ESI MS negative mode: calcd. for C.sub.12H.sub.22ClO.sub.10 [M+Cl].sup. m/z, 361.0902; found, 361.0884.

3-Deoxy-3-fluoro-,-D-trehalose (3-FluoroTre)

(65) From 3.6 mg of 3-FluoroGlc, obtained 6.5 mg 3-FluoroTre (96%). .sup.1H NMR (500 MHz, D.sub.2O): 5.23 (t, J=3.5 Hz, 1H), 5.17 (d, J=4.0 Hz, 1H), 4.74 (dt, J.sub.H,H=9.5 Hz, J.sub.H,F=55 Hz, 1H), 3.92 (m, 1H), 3.87-3.73 (m, 7H), 3.63 (dd, J=3.5, 9.5 Hz, 1H), 3.43 (t, J=9.5 Hz, 1H). .sup.13C NMR (125 MHz, D.sub.2O): 94.27 (d, J.sub.C,F=178 Hz), 93.43 (d, J.sub.C,F=10.5 Hz), 93.35, 72.41, 72.14, 71.59 (d, J.sub.C,F=6.6 Hz), 70.88, 69.53, 69.45 (d, J.sub.C,F=20 Hz), 67.80 (d, J.sub.C,F=17 Hz), 60.40, 60.00. HR ESI MS negative mode: calcd. for C.sub.12H.sub.21ClFO.sub.10 [M+Cl].sup. m/z, 379.0807; found, 379.0794.

6-Azido-6-Deoxy-,-D-trehalose (6-TreAz)

(66) From 4.1 mg of 6-GlcAz, obtained 6.7 mg 6-TreAz (92%). .sup.1H NMR (500 MHz, D.sub.2O): 5.05 (d, J=4.5 Hz, 1H), 5.04 (d, J=4.0 Hz, 1H), 3.82 (ddd, J=2.5, 6.0, 10.5 Hz, 1H), 3.72-3.67 (m, 4H), 3.61 (dd, J=5.0, 12 Hz, 1H), 3.55-3.49 (m, 3H), 3.42 (dd, J=5.5, 13.5 Hz, 1H), 3.31 (t, J=9.5 Hz, 1H), 3.30 (t, J=9.0 Hz, 1H). .sup.13C NMR (125 MHz, D.sub.2O): 93.53, 93.33, 72.43, 72.23, 72.12, 70.91, 70.89, 70.86, 70.38, 69.57, 60.40, 50.78. HR ESI MS negative mode: calcd. for C.sub.12H.sub.21ClN.sub.3O.sub.10 [M+Cl].sup. m/z, 402.0915; found, 402.0922.

5-Deoxy-5-Thio-,-D-trehalose (5-ThioTre)

(67) From 3.9 mg of 5-ThioGlc, obtained 6.5 mg 5-ThioTre (92%). .sup.1H NMR (500 MHz, D.sub.2O): 5.41 (d, J=4.0 Hz, 1H), 4.98 (d, J=3.5 Hz, 1H), 3.93 (dd, J=3.0, 10 Hz, 1H), 3.89-3.77 (m, 6H), 3.75 (dd, J=5.0, 11 Hz, 1H), 3.67-3.63 (m, 2H), 3.44 (t, J=9.5 Hz, 1H), 3.13 (m, 1H). .sup.13C NMR (125 MHz, D.sub.2O): 93.48, 76.99, 74.76, 73.62, 73.53, 72.71, 72.24, 70.92, 69.63, 60.43, 59.90, 42.87. HR ESI MS negative mode: calcd. for C.sub.12H.sub.22ClO.sub.10S [M+Cl].sup. m/z, 393.0622; found, 393.0606.

(68) One-Pot Metabolic Labeling and Imaging of M. smegmatis:

(69) Cultures of M. smegmatis wild type, sugC mutant, or sugC::sugC complement were generated by inoculating a single colony from a freshly streaked agar plate (with appropriate antibiotic, if needed) into 3 mL Middlebrook 7H9 liquid medium supplemented with ADC (albumin, dextrose, and catalase), 0.5% glycerol, and 0.05% Tween-80 in a culture tube. Starter cultures were incubated at 37 C. with shaking until reaching log phase. Meanwhile, using the procedure described above, a 50 L TreT reaction employing 6-GlcAz (10 mM) as the substrate was carried out to synthesize 6-TreAz (+TreT sample). A control reaction lacking TreT was run in parallel (TreT control). After incubation at 70 C. for 1 h, the reactions were stopped by placing on ice. Given the observed quantitative conversion for this reaction (FIG. 1B, Entry 16), a 6-TreAz concentration of 10 mM was assumed in the +TreT sample. For the TreT control, a 6-GlcAz concentration of 10 mM was assumed. The aqueous reaction mixtures were directly diluted into M. smegmatis wild type, sugC mutant, or sugC::sugC complement growing in 7H9 liquid medium to a final azido sugar concentration of 25 M and a culture density of OD.sub.600=0.15. Control experiments in which bacterial strains were cultured only in 7H9 liquid medium were run in parallel. Bacteria were incubated at 37 C. for 4 h (final densities of all cultures were OD.sub.600=0.60-0.64), after which cells were pelleted (5000 rpm for 5 min) and washed three times with phosphate-buffered saline supplemented with 0.5% BSA (PBS-B), then fixed by treatment with 4% paraformaldehyde in PBS for 15 min. Fixed cells were pelleted and washed once with PBS-B, then reacted with alkyne-modified carboxyrhodamine 110 fluorophore (Click Chemistry Tools) via Cu-catalyzed azide-alkyne cycloaddition (CuAAC) according to Breidenbach et al., Proc. Natl. Acad. Sci. U.S.A. (2010) 108, 3141 and Swarts et al., J. Am. Chem. Soc. (2012) 134, 16123, the entire contents of both of which are incorporated herein by reference. Following CuAAC, cells were pelleted and washed three times with PBS-B. 10 uL of bacteria resuspended in PBS were spotted onto a microscope slide, lightly spread into a thin layer using the edge of a coverslip, and allowed to air dry in the dark. Fluoromount-G mounting medium (SouthernBiotech) was applied, then cover slips were placed over the sample and immobilized with adhesive. Microscopy was carried out using an EVOS FL (Life Technologies) inverted microscope equipped with a 1001.4 numerical aperture Plan-Apochromat oil immersion lens. Fluorescence imaging was performed using a GFP LED light cube (maximum excitation/emission=470/510 nm). Images were captured with a Sony ICX445 CCD camera and processed using the FIJI distribution of Image. Image acquisition and processing were performed identically for all samples.

(70) Genetic Complementation of the M. smegmatis sugC Mutant Strain:

(71) The sugC gene (MSMEG_5058) was amplified from genomic DNA of M. smegmatis mc.sup.2155 by PCR using the oligonucleotide pair

(72) TABLE-US-00001 (SEQIDNO:1) 5-TTTTTTTAATTAAATGGCCGAAATTGTGTTGGATCG-3 and (SEQIDNO:2) 5-TTTTTAAGCTTCACCCGGCGTCCGGCCCCGCCG-3
and cloned using the restriction enzymes PacI and HindIII (underlined) into the single-copy integrative plasmid pMV361(Apra)-PacI, a derivative of pMV361(Kan) containing an apramycin resistance gene and a unique Pad restriction site. The resulting plasmid pMV361(Apra)::MSMEG_5058 was then transformed by electroporation into the M. smegmatis sugC mutant yielding the complemented mutant strain M, smegmatis sugC::sugC with stable chromosomal integration of the plasmid into the mycobacteriophage L5 attB site, providing constitutive sugC gene expression from the HSP60 promoter. Solid medium containing 50 mg/L hygromycin and 10 mg/L apramycin was used for selection.

Example 2: Trehalose Analogue Synthesis

(73) Studies were performed to synthesize trehalose analogues using trehalose synthase TreT from the hyperthermophile Thermoproteus tenax, for example, as shown in Scheme 1 below. Specifically, scheme 1 shows the synthesis of trehalose from Glc and UDP-Glc by TreT from T. tenax.

(74) ##STR00009##

(75) TreT was expressed and purified from E. coli and screened for reactivity using a panel of monofunctionalized Glc analogues (FIGS. 1A, 1B, and 1C). The Glc analogues that were tested contained fluoro-, deoxy-, azido-, and stereochemical modifications occurring at all positions of the sugar ring, which afforded a systematic evaluation of TreT substrate specificity. Reactions were performed in 50 mM HEPES buffer (pH 7.4) containing 10 mM Glc analogue, 40 mM UDP-Glc, 20 mM MgCl.sub.2, and 9.8 M TreT (reaction volume 50 L). The reactions were incubated at 70 C. with gentle shaking for 1 h, quenched by addition of cold acetone, and analyzed by HPLC and high-resolution ESI mass spectrometry as described above in Example 1.

(76) The Glc analogues which were evaluated were well-tolerated by TreT (FIGS. 1A, 1B, and 1C). In most cases, the corresponding trehalose analogue products were generated in excellent yield after only 1 h, which highlighted the efficiency, rapidity, and generality of the method. Fluoro-, deoxy-, azido-, and stereochemical modifications of the Glc 2-, 3-, and 6-positions were generally accepted, except for azido substitution at the 2-position and inversion of the 3-OH group. Glc analogues bearing 4-position alterations were poor substrates (N.D.26% yield), indicating a strict specificity at this position. Finally, 5-thio-D-glucose, the sole 5-position-modified Glc analogue that was tested, was converted to 5-thio-trehalose in quantitative yield.

(77) Selected reactions were run on a semi-preparative scale (5-10 mg) to evaluate the scalability of the method and to confirm product structure by NMR spectroscopy. Semi-preparative reactions were performed as described above and the products were readily purified by silica gel chromatography. Consistent with the small-scale results, 2-FluoroTre, 2-DeoxyTre, 3-FluoroTre, 6-TreAz, and 5-ThioTre were obtained in isolated yields of 92-97%. .sup.1H and .sup.13C NMR analysis established the product structures, including the assignment of 1,1-,-stereochemistry for newly formed glycosidic bonds (see Example 1).

Example 3: Single-Day Probe Synthesis, Metabolic Labeling, and Imaging

(78) To evaluate the applicability of the method described in Example 2 to imaging mycobacteria, a one-day experiment was performed that encompassed probe synthesis, metabolic labeling, and imaging of M. smegmatis, which is an avirulent model organism frequently used in tuberculosis (TB) research (FIG. 2A).

(79) First, TreT was used to convert commercially available 6-azido-6-deoxy-D-glucose (6-GlcAz) to 6-TreAz (FIG. 1B, Entry 16), an established chemical reporter for metabolic labeling of mycobacterial glycolipids. Next, the biocompatibility of enzymatic reactions was utilized by directly diluting the aqueous TreT reaction mixture into live M. smegmatis cell suspension, which is referred to herein as one-pot metabolic labeling. After incubation for 4 h, 6-TreAz-labeled mycobacteria were washed, fixed, and reacted with an azide-reactive fluorophore, alkyne-488, via Cu-catalyzed azide-alkyne cycloaddition (CuAAC).

(80) As shown in FIG. 2B, fluorescence microscopy revealed strong labeling of wild-type M. smegmatis that was treated with a reaction mixture containing 6-TreAz (+TreT). No fluorescence was observed when bacteria were treated with a reaction mixture lacking TreT (TreT), which consisted only of unreacted substrates.

(81) The same experiments were performed in M. smegmatis sugC, a mutant missing the trehalose transporter required for 6-TreAz uptake and labeling, as well as its complement, M. smegmatis sugC::sugC. Fluorescence was abolished in the sugC mutant and restored in the complement, confirming that 6-TreAz labeling proceeded via the trehalose transporter pathway.

(82) Additionally, direct treatment of M. smegmatis with TreT reaction mixture had no effect on bacterial growth or appearance. This operationally simple experiment provided a model for rapidly preparing and administering trehalose-based chemical probes. By contrast, traditional methods for synthesizing and purifying these compounds can require several weeks of work by a trained chemist in a well-equipped synthesis laboratory.

Example 4: Uptake of Fluoro Trehalose by the Trehalose Transporter in Mycobacteria

(83) Cultures of M. smegmatis wild type, sugC mutant, or sugC::sugC complement were generated by inoculating a single colony from a freshly streaked agar plate (with appropriate antibiotic, if needed) into 3 mL Middlebrook 7H9 liquid medium supplemented with ADC (albumin, dextrose, and catalase), 0.5% glycerol, and 0.05% Tween-80 in a culture tube. Starter cultures were incubated at 37 C. with shaking until reaching log phase. Cells were then cultured at 37 C. in the presence or absence of 2-, 3-, 4-, or 6-fluoro trehalose (100 M) in 7H9 liquid medium for 1 h, then centrifuged and washed (5000 rpm for 5 min) three times in ultrapure water. The washed cells were resuspended in ultrapure water and boiled for 4 h, then centrifuged. The supernatant was collected and dried on a speedvac to yield water-soluble cytosolic metabolites (e.g., trehalose and fluoro trehaloses, if present), which were subsequently trimethylsilyl (TMS)-derivatized using N-Methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA) in pyridine and analyzed by gas chromatography-mass spectrometry (GC-MS).

(84) FIGS. 3A-3D show gas chromatograms for uptake of purified 2-, 3-, 4-, and 6-fluoro trehalose. The corresponding fluoro trehalose derivative was observed in cytosolic extracts from wild-type M. smegmatis treated with 2-, 3-, and 6-fluoro trehalose, but not 4-fluoro trehalose. No uptake was observed for any analogues in the M. smegmatis sugC mutant, while uptake was restored for each analogue in the sugC::sugC complement. These data indicate that uptake of the fluoro trehalose analogues by mycobacteria is dependent on the trehalose transporter SugABC-LpqY. FIG. 3E shows gas chromatograms in a similar experiment, but in this case the 2-fluoro trehalose synthesized and administered using the rapid one-pot metabolic labeling method described above. Therefore, fluoro trehaloses can be rapidly synthesized by TreT in 60 minutes and immediately administered to mycobacteria, where they can selectively accumulate in the cytosol via the trehalose transporter.

(85) In summary, Examples 1-4 demonstrated that the thermostable trehalose synthase TreT from T. tenax converted a broad variety of Glc analogues into trehalose analogues in a single step in high yield (up to >99%) in 1 h. The TreT reaction was biocompatible and rapid (i.e., 1 h), and thus, allowed for administration of trehalose-based chemical probes to mycobacteria and imaging of mycobacteria with no effect on bacterial growth or appearance. Accordingly, probe synthesis, metabolic labeling, and imaging of the mycobacteria was accomplished in a single day.

(86) Various features and advantages of the invention are set forth in the following claims.