Ginkgolide B derivative and preparation method and use thereof

Abstract

Disclosed are a compound as shown in formula I or formula II or a pharmaceutically acceptable salt thereof, wherein R.sub.1 is selected from pyrazinyl or substituted pyrazinyl; and R.sub.2 is selected from pyrazinyl or substituted pyrazinyl, phenyl or substituted phenyl, alkyl or substituted alkyl.

Claims

1. A compound as shown in formula IIb or a pharmaceutically acceptable salt thereof: ##STR00023## wherein R.sub.32, R.sub.33, R.sub.34 and R.sub.35 are H simultaneously; and wherein R.sub.31 is selected from a C.sub.2-C.sub.6 ester group or ester group substituted alkyl as shown in formula A: ##STR00024## wherein R.sub.311 and R.sub.312 are separately or simultaneously selected from C.sub.2-C.sub.6 ester group or C.sub.1-C.sub.6 alkyl, and R.sub.311 and R.sub.312 are not C.sub.1-C.sub.6 alkyl simultaneously.

2. The compound or pharmaceutically acceptable salt thereof according to claim 1, characterized in that the pharmaceutically acceptable salt is selected from organic acid salts or inorganic acid salts.

3. A pharmaceutical composition, comprising the compound or pharmaceutically acceptable salt thereof according to claim 1 as an active ingredient, and comprising pharmaceutically acceptable accessories or auxiliary ingredients.

4. A pharmaceutical composition, comprising the compound or pharmaceutically acceptable salt thereof according to claim 2 as an active ingredient, and comprising pharmaceutically acceptable accessories or auxiliary ingredients.

Description

DETAILED DESCRIPTION

(1) Raw materials and equipment used in specific embodiments of the present invention are all known products and are purchased from commercially available products.

1. Acetylsalicylic acid: Chengdu Kelong Chemical Reagent Factory, LN 2013101601

2. Preparation of 3,5,6-trimethylpyrazine-2-formic Acid

(2) 10 g (73.42 mmol) of tetramethyl pyrazine is weighed and added into a 250 ml three-necked bottle, 100 ml of water is added into the three-necked bottle, heating is carried out to 35-40 C., 11.6 g of KMnO.sub.4 is added into the three-necked bottle with stirring, and a reaction is carried out for a period of 10 h while the temperature is preserved. Extraction with ethyl acetate is carried out, drying is carried out, and then, a solvent is removed to obtain 6.2 g of light-yellow solid, wherein the yield is 50.82%.

3. Preparation of S(+)-2-(2-chlorophenyl)-2-(4,5,6,7-tetrahydrothieno[3,2-c]pyridin-5)acetic acid

(3) 20 g of clopidogrel is weighed and added into a 500 ml three-necked bottle, 150 ml of methanol and 100 ml of 2 mol/L sodium hydroxide are added into the three-necked bottle, dissolving is carried out under stirring, then, heating is performed to 50 C., a reaction is carried out for a period of 1 h, depressurizing is performed to remove the methanol, then, 100 ml of water is added to dilute, cooling is performed to 0-10 C., the pH is adjusted to be acidic with hydrochloric acid, then, 150 ml of ethyl acetate is added, extracting and drying are performed, and then, the solvent is removed to obtain 13.2 g of off-white solid, wherein the yield is 69%.

4. Preparation of 2-bromomethyl-3,5,6-trimethylpyrazine

(4) Ligustrazine (20.0 g, 0.147 mol), NBS (26.8 g, 0.151 mol), a catalyst benzoyl peroxide (0.058 g, 0.232 mmol) and a solvent CCl.sub.4 (75.0 mL) are sequentially added into a 250 ml three-necked bottle, irradiating with an incandescent lamp is performed, heating is performed to 75 C. in an oil bath, and a reaction is performed for a period of 10 hours. Filtering is performed to remove the solvent from filter liquor to obtain a concentrate. Purification is performed by column chromatography to obtain 15.8 g of light-yellow solid, i.e., 2-bromomethyl-3,5,6-trimethylpyrazine, wherein the yield is 50%.

5. Preparation of 2-(1-acetoxylamyl)benzoic Acid

(5) 12.4 g (65 mmol) of butylphthalide is dissolved in a 500 mL single-necked flask containing 100 mL of methanol, 100 mL of 2 mol/L NaOH solution is added, stirring is performed for a period of 1 h at a temperature of 50 C., reduced pressure distillation is performed to remove the methanol, then, 100 mL of distilled water is added to dilute, cooling is performed to 5 C., the pH is adjusted to be 2 to 3 with 5% hydrochloric acid with stirring, extraction is performed with ethyl ether, drying is performed, and then, the ethyl ether is removed at a low temperature to obtain a white solid. 20 mL of triethylamine, 2.5 g of DMAP and 200 mL of dichloromethane are separately added into the white solid in a 500 mL three-necked bottle, stirring is performed to dissolve at a temperature of 10 C. to 0 C., then, 11 mL of acetyl chloride is dropwise added, and stirring is performed for a period of 5 h while preserving the temperature. 100 mL of water is added, stirring is performed for a period of 0.5 h at room temperature, an organic layer is separated out, drying is performed, then, the solvent is removed, and purifying is performed to obtain 6.35 g of white solid, wherein the yield is 38.96%.

(6) Similarly, 3,5,6-trimethylpyrazine-2-formic acid, 2-bromomethyl-3,5,6-trimethylpyrazine and 2-(1-acetoxylamyl)benzoic acid all can be purchased from the market.

Embodiment 1: Preparation of Compound BZ

(7) ##STR00018##

(8) 5.0 g (11.78 mmol) of GB and 2.55 g (15.32 mmol) of 3,5,6-trimethylpyrazine-2-formic acid are weighed and dissolved in acetonitrile, and stirring and mixing are performed in an ice bath. Then, 0.29 g (2.36 mmol) of 4-dimethylaminopyridine (DMAP) and 3.17 g (16.49 mmol) of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC.HCl) are added, stirring is performed for a period of 1 hour in the ice bath, then, the mixture is subjected to a reaction for a period of 6 h at a temperature of 20 C., rotary evaporation is performed to remove the solvent, a coarse product is dissolved with ethyl acetate, the product is washed twice with 5% NaHCO.sub.3, and then the product is washed once with a saturated sodium chloride solution. An organic phase is collected, drying, filtering and concentrating are performed, and separating and purifying are performed to obtain 2.80 g of white solid BZ in all, wherein the yield is 41.48%, and the HPLC purity is 99.80%.

(9) LC-MS: 573.2[M+H.sup.+], 595.2[M+Na.sup.+].

(10) .sup.1H-NMR (DMSO, 400 MHz): 1.02 (s, 9H, t-Bu), 1.13-1.19 (d, 3H, 14-Me), 1.75-1.79 (dd, 1H, 8-H), 1.84-1.89 (d, 1H, 7-H), 2.16-2.21 (q, 1H; 7-H), 2.49-2.58 (dd, 6H, 2CH.sub.3-pyrazine), 2.76 (s, 3H, CH.sub.3-pyrazine), 2.86-2.91 (q, 1H, 14-H), 4.15-4.18 (q, 1H, 1-H), 4.72-4.74 (d, 1H, 2-H), 5.46-5.47 (d, 1H, 1-OH), 6.33 (s, 1H, 10-H), 6.50 (s, 1H, 6-H), 6.57 (s, 1H, 12-H), 6.89-6.90 (d, 1H, 3-OH).

Embodiment 2: Preparation of Compound BA

(11) ##STR00019##

(12) 5.0 g (11.78 mmol) of GB and 2.76 g (15.32 mmol) of acetylsalicylic acid are weighed and dissolved in acetonitrile, and stirring and mixing are performed in an ice bath. Then, 0.29 g (2.36 mmol) of 4-dimethylaminopyridine (DMAP) and 3.17 g (16.49 mmol) of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC.HCl) are added, stirring is performed for a period of 1 hour in the ice bath, then, the mixture is subjected to a reaction for a period of 4 h at a temperature of 30 C., rotary evaporation is performed to remove the solvent, a coarse product is dissolved with ethyl acetate, the product is washed twice with 5% NaHCO.sub.3, and then the product is washed once with saturated sodium chloride. An organic phase is collected, drying, filtering and concentrating are performed, and separating and purifying are performed to obtain 2.52 g of white solid BA in all, wherein the yield is 36.52%, and the HPLC purity is 99.13%.

(13) MS: 609.16[M+Na.sup.+], C.sub.29H.sub.30NaO.sub.13.

(14) .sup.1H-NMR (CDCl.sub.3, 400 MHz): 1.08 (s, 9H, t-Bu), 1.24-1.26 (d, 3H, 14-Me), 1.99-2.03 (dd, 1H, 8-H), 2.16 (s, 3H, CH.sub.3CO), 2.24 (d, 1H, 7-H), 2.30-2.37 (q, 1H, 7-H), 3.01-3.09 (q, 1H, 14-H), 4.08-4.13 (q, 1H, 1-H), 4.20-4.22 (d, 1H, 2-H), 4.63-4.67 (d, 1H, 1-OH), 5.64-5.65 (s, 1H, 10-H), 6.14 (s, 1H, 6-H), 6.21 (s, 1H, 12-H), 6.91-7.99 (ddd, 4H, Ar), 10.03 (s, 1H, 3-OH).

Embodiment 3: Preparation of Compound BL

(15) ##STR00020##

(16) 5.0 g (11.78 mmol) of GB and 4.70 g (15.32 mmol) of S(+)-2-(2-chlorophenyl)-2-(4,5,6,7-tetrahydrothieno[3,2-c]pyridin-5)acetic acid are weighed and dissolved in acetonitrile, and stirring and mixing are performed in an ice bath. Then, 0.29 g (2.36 mmol) of 4-dimethylaminopyridine (DMAP) and 3.17 g (16.49 mmol) of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC.HCl) are added, stirring is performed for a period of 1 hour in the ice bath, and then, the mixture is subjected to a reaction for a period of 5 h at a temperature of 25 C. Rotary evaporation is performed to remove the solvent, a coarse product is dissolved with ethyl acetate, the product is washed twice with 5% NaHCO.sub.3, and then the product is washed once with saturated sodium chloride. An organic phase is collected, drying, filtering and concentrating are performed, and separating and purifying are performed to obtain 3.46 g of white solid BL in all, wherein the yield is 41.09%, and the HPLC purity is 99.58%.

(17) LC-MS: 714.3[M+H.sup.+], 736.0[M+Na.sup.+].

(18) .sup.1H-NMR (CDCl.sub.3, 400 MHz): 1.05 (s, 9H, t-Bu), 1.25-1.36 (d, 3H, 14-Me), 1.78-1.83 (dd, 1H, 8-H), 2.00-2.04 (m, 4H, CH.sub.2CH.sub.2), 2.28-2.35 (d, 1H, 7-H), 2.82 (q, 1H, 7-H), 2.94-3.01 (q, 1H, 14-H), 3.86-3.87 (d, 2H, CH.sub.2), 4.09 (s, 1H, CHCO), 4.11-4.14 (q, 1H, 1-H), 4.17-4.18 (d, 1H, 2-H), 4.51-4.53 (d, 1H, 1-OH), 5.41-5.43 (s, 1H, 10-H), 5.43-5.45 (d, 1H, 6-H), 6.08 (s, 1H, 12-H), 6.23 (s, 1H, 3-OH), 6.67-6.69 (dd, 1H, CHS), 7.15-7.18 (dd, 1H, CHCHS), 7.36-7.68 (m, 4H, Ar).

Embodiment 4: Preparation of Compound BM

(19) ##STR00021##

(20) 2.0 g of ginkgolide B is dissolved in 50 ml of acetonitrile, 0.96 g of 2-bromomethyl-3,5,6-trimethylpyrazine (1.2 eq), 6.81 g of potassium carbonate (10 eq) and a small amount of KI catalyst are sequentially added, and a reaction is performed at a temperature of 60 C. until the raw material ginkgolide B reacts completely. Cooling is performed to room temperature, filtering is performed, and then, filter liquor is subjected to rotary evaporation to obtain a light-yellow oily matter. Column-chromatography purification (V petroleum ether:V ethyl acetate=2:1) is performed to obtain 1.05 g of white solid, i.e., BM, wherein the yield is 39.92%, and the HPLC purity is 98.25%.

(21) LC-MS: 559.3[M+H.sup.+], 581.3[M+Na.sup.+].

(22) .sup.1H-NMR (DMSO, 400 MHz): 1.14 (s, 9H, t-Bu), 1.16-1.20 (d, 3H, 14-Me), 1.80-1.85 (dd, 2H, 7-H), 2.15-2.8 (t, 1H, 8-H), 2.42 (s, 3H, CH.sub.3), 2.49-2.53 (d, 6H, CH.sub.3), 2.82-2.88 (q, 1H, 14-H), 4.17-4.19 (d, 1H, 1-H), 4.71-4.73 (d, 1H, 2-H), 4.94-4.98 (d, 1H, 1-OH), 5.41 (s, 1H, CH.sub.2), 5.42 (s, 1H, 10-H), 5.43-5.45 (d, 1H, 6-H), 5.54 (s, 1H, 12-H), 6.23 (s, 1H, 3-OH).

Embodiment 5: Preparation of Compound BD

(23) ##STR00022##

(24) 5.0 g (11.78 mmol) of GB and 3.83 g (15.32 mmol) of 2-(1-acetoxylamyl)benzoic acid are weighed and dissolved in acetonitrile, and stirring and mixing are performed in an ice bath. Then, 0.29 g (2.36 mmol) of 4-dimethylaminopyridine (DMAP) and 3.17 g (16.49 mmol) of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC.HCl) are added, stirring is performed for a period of 1 hour in the ice bath, then, the mixture is subjected to a reaction for a period of 5 h at a temperature of 25 C., rotary evaporation is performed to remove the solvent, a coarse product is dissolved with ethyl acetate, the product is washed twice with 5% NaHCO.sub.3, and then the product is washed once with saturated sodium chloride. An organic phase is collected, drying, filtering and concentrating are performed, and separating and purifying are performed to obtain 2.90 g of white solid BD in all, wherein the yield is 37.46%, and the HPLC purity is 98.14%.

(25) LC-MS: 679.2[M+Na.sup.+].

(26) .sup.1H-NMR (CDCl.sub.3, 400 MHz): 0.87-0.91 (t, 3H, CH.sub.3), 1.12 (s, 9H, t-Bu), 1.27-1.29 (d, 3H, 14-Me), 1.32-1.36 (dd, 2H, 7-H), 1.45-1.49 (t, 1H, 8-H), 1.75-1.92 (m, 4H, CH.sub.2CH.sub.2), 1.98 (s, 5H, COCH.sub.3, CH.sub.2CH.sub.3), 2.37-2.39 (q, 1H, 14-H), 3.03-3.09 (q, 2H, 1-H, 2-H), 4.37-4.39 (d, 1H, 1-OH), 4.54-4.56 (d, 1H, 10-H), 5.50-5.51 (d, 1H, 6-H), 5.89-5.94 (m, 1H, CHCH.sub.2), 6.13 (s, 1H, 12-H), 6.23 (s, 1H, 3-OH), 7.32-7.38 (m, 2H, ArH), 7.46-7.48 (d, 1H, ArH), 7.50-7.54 (d, 1H, ArH).

Embodiment 6: Preparation of BZ Methanesulfonate

(27) 2.0 g of BZ is added into 50 mL of acetone, heating is performed to a temperature of 40-50 C. with stirring, 0.44 g of methanesulfonic acid is slowly dropwise added into a solution after the solution is clarified, the solution is cooled after dropwise adding is completed, stirring is performed for a period of 30 min, and filtering and drying are performed to obtain 1.85 g of BZ methanesulfonate, wherein the yield is 79.06%.

Embodiment 7: Preparation of BM Methanesulfonate

(28) 0.9 g of BM is added into 30 mL of acetone, heating is performed to a temperature of 40-50 C. with stirring, 0.19 g of methanesulfonic acid is slowly dropwise added into a solution after the solution is clarified, the solution is cooled after dropwise adding is completed, stirring is performed for a period of 30 min, and filtering and drying are performed to obtain 0.82 g of BM methanesulfonate, wherein the yield is 81.56%.

Embodiment 8: Preparation of BZ Hydrochloride

(29) 1.0 g of BZ is added into 30 mL of anhydrous ethyl alcohol, heating is performed to a temperature of 60-70 C. with stirring, an ethanol solution of hydrogen chloride (with the content of 30-40%) is slowly dropwise added into a solution after the solution is clarified until the pH of the solution is about 3, cooling is performed after the dropwise adding is completed, the material is allowed to stand to crystallize so as to separate out a white solid, filtering is performed, and then drying is performed to obtain 0.72 g of BZ hydrochloride, wherein the yield is 67.92%.

Embodiment 9: Preparation of BM Hydrochloride

(30) 1.5 g of BM is added into 50 mL of anhydrous ethyl alcohol, heating is performed to a temperature of 60-70 C. with stirring, an ethanol solution of hydrogen chloride (with the content of 30-40%) is slowly dropwise added into a solution after the solution is clarified until the pH of the solution is about 3, cooling is performed after the dropwise adding is completed, the material is allowed to stand to crystallize so as to separate out a white solid, filtering is performed, and then drying is performed to obtain 0.95 g of BM hydrochloride, wherein the yield is 59.38%.

(31) Beneficial effects of the present invention are described below in a test example manner.

Text Example 1: Water Solubility Test

(32) Measuring method: samples are ground to obtain fine powder, target compounds are quantitatively weighed, the weighed compounds are added into purified water, and the solubility of the compounds is investigated by ultrasonics. A result is as shown in a table 1.

(33) TABLE-US-00001 TABLE 1 Solubility of each target compound in water Compound Solubility in water (mg/mL) Ginkgolide B (GB) 0.11 BM 0.25 BM methanesulfonate 4.20 BM hydrochloride 4.65 BZ 0.30 BZ methanesulfonate 5.02 BZ hydrochloride 5.10 BA 0.22 BL 0.20 BD 0.25

(34) The result shows that water solubility of ginkgolide B derivatives and salts thereof is obviously improved compared with that of ginkgolide B.

Text Example 2: Pharmacological Test of Ginkgolide B Derivatives on Pressure Lowering of Primary Hypertension

(35) The primary hypertension is a genetic heterogeneity disease, and the hypertension is generated by a plurality of genes with weak effects through joint action and is affected by a series of environmental factors. A pathologic process of the hypertension often relates to artery blood vessel wall thickening, myocardial fibrosis, left ventricular hypertrophy and nephro-angiosclerosis, the injury to hypertension target organs is caused, the incidence rate and death rate of cardiovascular and cerebrovascular incidents and nephropathy are obviously increased, and thus, the hypertension is extremely dangerous to human Therefore, reasonable control of blood pressure has a very important clinical significance.

(36) Spontaneously hypertensive rats (SHR) have many similarities with human primary hypertension, including hereditary character, pathogenesis process, occurrence of hypertension complication, and thus, the SHR rats are reputed as optimal animal models for researching the human primary hypertension and have been extensively applied to experimental researches on hypertension pathogenesis and curative effect of anti-hypertension drugs. Therefore, the SHR rats are selected as test animals in the test.

(37) 1. Test Materials

(38) 1.1 Test drugs: BZ, BA, BL, BM, BD and GB, purity>98%.

(39) 1.2 Positive control drug: Captopril Tablets, 25 mg/tablet, Shanghai pukang pharmaceutical Co., Ltd., Lot Number: 101003.

(40) 1.3 Test instruments: BP-6 non-invasive animal blood pressure measuring system (Chengdu Taimeng Technology Co., Ltd.); FA1004 electronic analytical balance (Shanghai Precision Scientific Instrument Co., Ltd.).

(41) 1.4 Test animals: 112 SHR rats with the age of 14 weeks, wherein the female and male SHR rats are half and half, have the body weight of (200-250) g and are provided by Beijing Vital River Laboratory Animal Technology Co., Ltd., and a laboratory animal production license number is SCXK (Beijing) 2007-001; 8 healthy Wistar rats with the same weeks of age, wherein the female and male Wistar rats are half and half, have the body weight of (200-250) g and are provided by animal center of Nanjing medical college, and a laboratory animal production license number is SCXK (Suzhou) 2008-0004.

(42) 2. Test Method

(43) 2.1 Grouping and administration of animals: 112 SHR rats (the female and male SHR rats are half and half, and the female rats are not pregnant) with the body weight of 200-250 g are taken, the rats are enabled to adapt to a laboratory for a period of 5 d, blood pressure is measured once at a day to enable the rats to adapt to the environment and detect stimulation. Then, weighing and numbering are carried out, and the SHR rats are equally divided into 14 groups randomly (each group comprises 8 rats), i.e., an SHR rat model control group, a positive control group (captopril, 27 mg/kg), a BZ large-dose group (120 mg/kg/d), a BZ small-dose group (60 mg/kg/d), a BA large-dose group (120 mg/kg/d), a BA small-dose group (60 mg/kg/d), a BL large-dose group (120 mg/kg/d), a BL small-dose group (60 mg/kg/d), a BM large-dose group (120 mg/kg/d), a BM small-dose group (60 mg/kg/d), a BD large-dose group (120 mg/kg/d), a BD small-dose group (60 mg/kg/d), a GB large-dose group (120 mg/kg/d) and a GB small-dose group (60 mg/kg/d). In addition, 8 healthy Wistar rats are taken as a normal control group. Intragastric administration is performed on the rats of each drug group separately according to the above-mentioned doses by 1 mL/100 g body weight, intragastric administration is performed on the rats of the normal control group and the rats of the SHR rat model control group with an equal volume of distilled water, and administration is performed continuously for 8 weeks.

(44) 2.2 General state: during observation, conditions such as mind, water drinking, food intake, bowel movement, hair color, body weight and range of motion of rats are observed. Irritable degrees of the rats are divided into III levels: a level I means that the rats are free of obvious response when necks of the rats are seized; a level II means that the rats scream and startle when the necks of the rats are seized; and a level III means that the rats bite or rats of same cages frequently fight when the necks of the rats are seized.

(45) 2.3 Blood pressure measuring: caudal artery systolic pressure of rats is measured with a Bp-6 noninvasive blood pressure measuring system. Under the condition that the rats are calm, the systolic pressure is continuously measured for 3 times, and the average value of the measured systolic pressure is taken as a pressure measuring result. Observation indexes: {circle around (1)} single-administration pressure lowering effect: blood pressure of the rats is measured before administration and blood pressure conditions are measured 30 min, 60 min, 90 min and 120 min after first administration; and {circle around (2)} continuous-administration pressure lowering condition: blood pressure is separately measured 30 min after administration at 1 d, 7 d, 13 d, 19 d, 25 d, 35 d, 49 d and 56 d, the blood pressure is repeatedly measured for 3 times at a day, and the average value of obtained values is taken as a final blood pressure value.

(46) 2.4 Data statistical method: a test data result is represented by meanstandard deviation (xs), one-factor variance analysis check is carried out by adopting a software SPSS15, and P<0.05 shows that the difference has a remarkable statistics significance.

(47) 3 Test Result

(48) 3.1 Influence on general state of spontaneously hypertensive rats: the rats of the normal control group are normal in food intake, drinking and motion conditions, sensitive in response, healthy and glossy in hair color, good in mental state and free of any unusual response; the rats of the SHR model group gradually present that back hair is fluffy, clustered and dim, the appetite is reduced, the body weight is reduced, the mind is dispirited and irritable, and the rats are prone to the behaviors of intense resisting, attacking and so on during administration and blood pressure detection, and irritable degrees are mostly changed to a level II and a level III from a level I; and after single administration of ginkgolide B and derivatives thereof, behavioral general state observations of rats of each group are free of obvious difference from those of the model control group. After 2 weeks of continuous-administration treatment, mental states are obviously changed for the better, emotions are relatively stable, the food intake is obviously increased, and the body weight is increased faster. The food intake and body weight of rats of each group are weighed once per week, the food intake of the SHR model group is obviously reduced, the body weight of the SHR model group is reduced, and the SHR model group has remarkable difference (P<0.05 or P<0.01) compared with the normal control group; for high-dose groups of the ginkgolide B and derivatives thereof, the food intake and body weight of the SHR rats can be remarkably increased, and the high-dose groups have remarkable difference (P<0.05 or P<0.01) from the SHR model control group; and results are shown in table 2 and table 3.

(49) TABLE-US-00002 TABLE 2 Influence on weekly food intake of SHR rats Week 0 1 2 3 4 5 6 7 8 Group Average dairy food intake g/d Normal control 18.5 19.3 20.4 21.0 21.5 23.2 25.0 26.7 28.1 group Model control group 18.2 16.5* 16.3* 16.6** 16.9** 17.4** 19.6** 21.2** 22.0** Positive control 18.4 20.0.sup..box-tangle-solidup. 19.1.sup..box-tangle-solidup. 19.5.sup..box-tangle-solidup. 19.9.sup..box-tangle-solidup. 21.4.sup..box-tangle-solidup..box-tangle-solidup. 23.8.sup..box-tangle-solidup..box-tangle-solidup. 24.6.sup..box-tangle-solidup..box-tangle-solidup. 26.3.sup..box-tangle-solidup..box-tangle-solidup. group GB high-dose group 18.3 20.6.sup..box-tangle-solidup..box-tangle-solidup. 19.5.sup..box-tangle-solidup. 20.6.sup..box-tangle-solidup..box-tangle-solidup. 21.4.sup..box-tangle-solidup..box-tangle-solidup. 22.8.sup..box-tangle-solidup..box-tangle-solidup. 24.6.sup..box-tangle-solidup..box-tangle-solidup. 26.8.sup..box-tangle-solidup..box-tangle-solidup. 28.2.sup..box-tangle-solidup..box-tangle-solidup. GB low-dose group 18.6 20.1.sup..box-tangle-solidup. 18.9.sup..box-tangle-solidup. 17.7 18.6.sup..box-tangle-solidup. 21.7.sup..box-tangle-solidup..box-tangle-solidup. 23.5.sup..box-tangle-solidup..box-tangle-solidup. 24.2.sup..box-tangle-solidup..box-tangle-solidup. 25.4.sup..box-tangle-solidup..box-tangle-solidup. BA high-dose group 18.1 19.8.sup..box-tangle-solidup. 20.1.sup..box-tangle-solidup..box-tangle-solidup. 20.8.sup..box-tangle-solidup..box-tangle-solidup. 21.6.sup..box-tangle-solidup..box-tangle-solidup. 23.0.sup..box-tangle-solidup..box-tangle-solidup. 24.5.sup..box-tangle-solidup..box-tangle-solidup. 25.9.sup..box-tangle-solidup..box-tangle-solidup. 27.8.sup..box-tangle-solidup..box-tangle-solidup. BA low-dose group 17.9 18.6.sup..box-tangle-solidup. 19.8.sup..box-tangle-solidup. 19.6.sup..box-tangle-solidup. 18.9.sup..box-tangle-solidup. 20.7.sup..box-tangle-solidup..box-tangle-solidup. 22.3.sup..box-tangle-solidup..box-tangle-solidup. 24.6.sup..box-tangle-solidup..box-tangle-solidup. 25.5.sup..box-tangle-solidup..box-tangle-solidup. BD high-dose group 18.3 20.1.sup..box-tangle-solidup. 20.6.sup..box-tangle-solidup..box-tangle-solidup. 21.0.sup..box-tangle-solidup..box-tangle-solidup. 21.5.sup..box-tangle-solidup..box-tangle-solidup. 22.8.sup..box-tangle-solidup..box-tangle-solidup. 23.9.sup..box-tangle-solidup..box-tangle-solidup. 25.4.sup..box-tangle-solidup..box-tangle-solidup. 26.9.sup..box-tangle-solidup..box-tangle-solidup. BD low-dose group 18.7 19.0.sup..box-tangle-solidup. 19.4.sup..box-tangle-solidup. 19.8.sup..box-tangle-solidup. 20.0.sup..box-tangle-solidup..box-tangle-solidup. 21.3.sup..box-tangle-solidup..box-tangle-solidup. 22.6.sup..box-tangle-solidup..box-tangle-solidup. 24.3.sup..box-tangle-solidup..box-tangle-solidup. 25.0.sup..box-tangle-solidup..box-tangle-solidup. BZ high-dose group 18.0 19.3.sup..box-tangle-solidup. 20.5.sup..box-tangle-solidup..box-tangle-solidup. 22.0.sup..box-tangle-solidup..box-tangle-solidup. 22.6.sup..box-tangle-solidup..box-tangle-solidup. 23.6.sup..box-tangle-solidup..box-tangle-solidup. 24.1.sup..box-tangle-solidup..box-tangle-solidup. 24.9.sup..box-tangle-solidup..box-tangle-solidup. 27.0.sup..box-tangle-solidup..box-tangle-solidup. BZ low-dose group 18.1 19.5.sup..box-tangle-solidup. 19.7.sup..box-tangle-solidup. 20.4.sup..box-tangle-solidup..box-tangle-solidup. 20.9.sup..box-tangle-solidup..box-tangle-solidup. 21.7.sup..box-tangle-solidup..box-tangle-solidup. 22.9.sup..box-tangle-solidup..box-tangle-solidup. 24.0.sup..box-tangle-solidup..box-tangle-solidup. 24.9.sup..box-tangle-solidup..box-tangle-solidup. BL high-dose group 18.6 20.0.sup..box-tangle-solidup. 20.8.sup..box-tangle-solidup..box-tangle-solidup. 21.9.sup..box-tangle-solidup..box-tangle-solidup. 22.7.sup..box-tangle-solidup..box-tangle-solidup. 23.5.sup..box-tangle-solidup..box-tangle-solidup. 24.6.sup..box-tangle-solidup..box-tangle-solidup. 25.1.sup..box-tangle-solidup..box-tangle-solidup. 26.7.sup..box-tangle-solidup..box-tangle-solidup. BL low-dose group 18.4 19.4.sup..box-tangle-solidup. 20.5.sup..box-tangle-solidup..box-tangle-solidup. 21.0.sup..box-tangle-solidup..box-tangle-solidup. 20.9.sup..box-tangle-solidup..box-tangle-solidup. 21.9.sup..box-tangle-solidup..box-tangle-solidup. 23.5.sup..box-tangle-solidup..box-tangle-solidup. 24.5.sup..box-tangle-solidup..box-tangle-solidup. 25.1.sup..box-tangle-solidup..box-tangle-solidup. BM high-dose group 18.5 19.9.sup..box-tangle-solidup. 20.7.sup..box-tangle-solidup..box-tangle-solidup. 22.6.sup..box-tangle-solidup..box-tangle-solidup. 22.9.sup..box-tangle-solidup..box-tangle-solidup. 23.7.sup..box-tangle-solidup..box-tangle-solidup. 24.4.sup..box-tangle-solidup..box-tangle-solidup. 25.8.sup..box-tangle-solidup..box-tangle-solidup. 27.6.sup..box-tangle-solidup..box-tangle-solidup. BM low-dose group 18.2 19.1.sup..box-tangle-solidup. 19.9.sup..box-tangle-solidup. 20.9.sup..box-tangle-solidup..box-tangle-solidup. 21.3.sup..box-tangle-solidup..box-tangle-solidup. 22.4.sup..box-tangle-solidup..box-tangle-solidup. 23.1.sup..box-tangle-solidup..box-tangle-solidup. 24.7.sup..box-tangle-solidup..box-tangle-solidup. 25.5.sup..box-tangle-solidup..box-tangle-solidup. Note: comparing the model control group with the normal control group, *P < 0.05, **P < 0.01; and Comparing each drug group with the model control group, .sup..box-tangle-solidup.P < 0.05, .sup..box-tangle-solidup..box-tangle-solidup.P < 0.01.

(50) TABLE-US-00003 TABLE 3 Influence on weekly body weight of SHR rats Week 0 1 2 3 4 5 6 7 8 Group Body weight g Normal control 231.2 250.3 261.5 285.6 295.4 310.7 331.6 345.4 361.7 group Model control group 232.4 235.7* 240.6* 250.8** 255.7** 260.4** 265.7** 271.5** 275.6** Positive control 230.5 235.1 245.4.sup..box-tangle-solidup. 255.9.sup..box-tangle-solidup..box-tangle-solidup. 260.5.sup..box-tangle-solidup..box-tangle-solidup. 266.3.sup..box-tangle-solidup..box-tangle-solidup. 271.0.sup..box-tangle-solidup..box-tangle-solidup. 280.6.sup..box-tangle-solidup..box-tangle-solidup. 290.7.sup..box-tangle-solidup..box-tangle-solidup. group GB high-dose group 231.0 250.3.sup..box-tangle-solidup..box-tangle-solidup. 258.7.sup..box-tangle-solidup..box-tangle-solidup. 263.4.sup..box-tangle-solidup..box-tangle-solidup. 270.6.sup..box-tangle-solidup..box-tangle-solidup. 281.5.sup..box-tangle-solidup..box-tangle-solidup. 290.6.sup..box-tangle-solidup..box-tangle-solidup. 306.7.sup..box-tangle-solidup..box-tangle-solidup. 321.2.sup..box-tangle-solidup..box-tangle-solidup. GB low-dose group 230.8 235.6 243.1.sup..box-tangle-solidup. 250.2 258.9.sup..box-tangle-solidup. 262.4 268.7.sup..box-tangle-solidup. 280.6.sup..box-tangle-solidup..box-tangle-solidup. 290.1.sup..box-tangle-solidup..box-tangle-solidup. BA high-dose group 231.6 251.4.sup..box-tangle-solidup..box-tangle-solidup. 257.6.sup..box-tangle-solidup..box-tangle-solidup. 264.1.sup..box-tangle-solidup..box-tangle-solidup. 271.2.sup..box-tangle-solidup..box-tangle-solidup. 280.6.sup..box-tangle-solidup..box-tangle-solidup. 291.2.sup..box-tangle-solidup..box-tangle-solidup. 305.9.sup..box-tangle-solidup..box-tangle-solidup. 320.4.sup..box-tangle-solidup..box-tangle-solidup. BA low-dose group 228.5 233.6 244.8.sup..box-tangle-solidup. 251.4 257.3 263.7.sup..box-tangle-solidup. 269.6.sup..box-tangle-solidup. 281.8.sup..box-tangle-solidup..box-tangle-solidup. 292.8.sup..box-tangle-solidup..box-tangle-solidup. BD high-dose group 230.9 252.0.sup..box-tangle-solidup..box-tangle-solidup. 257.5.sup..box-tangle-solidup..box-tangle-solidup. 263.9.sup..box-tangle-solidup..box-tangle-solidup. 270.8.sup..box-tangle-solidup..box-tangle-solidup. 281.2.sup..box-tangle-solidup..box-tangle-solidup. 291.0.sup..box-tangle-solidup..box-tangle-solidup. 304.7.sup..box-tangle-solidup..box-tangle-solidup. 321.7.sup..box-tangle-solidup..box-tangle-solidup. BD low-dose group 229.2 234.5 247.1.sup..box-tangle-solidup..box-tangle-solidup. 252.2 257.2 262.4 270.4.sup..box-tangle-solidup. 280.2.sup..box-tangle-solidup..box-tangle-solidup. 290.5.sup..box-tangle-solidup..box-tangle-solidup. BZ high-dose group 268.9 249.4.sup..box-tangle-solidup..box-tangle-solidup. 256.7.sup..box-tangle-solidup..box-tangle-solidup. 262.4.sup..box-tangle-solidup..box-tangle-solidup. 268.9.sup..box-tangle-solidup..box-tangle-solidup. 280.7.sup..box-tangle-solidup..box-tangle-solidup. 291.3.sup..box-tangle-solidup..box-tangle-solidup. 305.4.sup..box-tangle-solidup..box-tangle-solidup. 320.9.sup..box-tangle-solidup..box-tangle-solidup. BZ low-dose group 231.0 235.7 242.5 251.6 259.4.sup..box-tangle-solidup. 263.6.sup..box-tangle-solidup. 269.1.sup..box-tangle-solidup..box-tangle-solidup. 276.8.sup..box-tangle-solidup..box-tangle-solidup. 288.0.sup..box-tangle-solidup..box-tangle-solidup. BL high-dose group 230.6 251.8.sup..box-tangle-solidup..box-tangle-solidup. 257.3.sup..box-tangle-solidup..box-tangle-solidup. 264.1.sup..box-tangle-solidup..box-tangle-solidup. 270.5.sup..box-tangle-solidup..box-tangle-solidup. 281.7.sup..box-tangle-solidup..box-tangle-solidup. 290.3.sup..box-tangle-solidup..box-tangle-solidup. 302.8.sup..box-tangle-solidup..box-tangle-solidup. 315.2.sup..box-tangle-solidup..box-tangle-solidup. BL low-dose group 230.9 236.4 243.1 252.8 260.9.sup..box-tangle-solidup..box-tangle-solidup. 265.8.sup..box-tangle-solidup..box-tangle-solidup. 270.6.sup..box-tangle-solidup. 277.3.sup..box-tangle-solidup..box-tangle-solidup. 285.4.sup..box-tangle-solidup..box-tangle-solidup. BM high-dose group 233.4 249.5.sup..box-tangle-solidup..box-tangle-solidup. 256.8.sup..box-tangle-solidup..box-tangle-solidup. 264.3.sup..box-tangle-solidup..box-tangle-solidup. 271.6.sup..box-tangle-solidup..box-tangle-solidup. 280.4.sup..box-tangle-solidup..box-tangle-solidup. 290.5.sup..box-tangle-solidup..box-tangle-solidup. 305.7.sup..box-tangle-solidup..box-tangle-solidup. 315.8.sup..box-tangle-solidup..box-tangle-solidup. BM low-dose group 231.5 236.8 245.7.sup..box-tangle-solidup..box-tangle-solidup. 253.4 258.3 263.5.sup..box-tangle-solidup. 271.3.sup..box-tangle-solidup..box-tangle-solidup. 281.6.sup..box-tangle-solidup..box-tangle-solidup. 292.5.sup..box-tangle-solidup..box-tangle-solidup. Note: comparing the model control group with the normal control group, *P < 0.05, **P < 0.01; and Comparing each drug group with the model control group, .sup..box-tangle-solidup.P < 0.05, .sup..box-tangle-solidup..box-tangle-solidup.P < 0.01.

(51) 3.2 Pressure Lowering Effect on Spontaneously Hypertensive Rats

(52) 3.2.1 Influence on Caudal Artery Systolic Pressure of Spontaneously Hypertensive Rats Caused by Single Administration

(53) A test result shows that the caudal artery pressure of the spontaneously hypertensive rats is obviously higher than that of the normal control group and has high-remarkable difference (P<0.01) from that of the normal control group; 30 min after single administration in each drug group, an obvious pressure lowering action on the spontaneously hypertensive rats is achieved, the systolic pressure has an obvious lowering trend at each time point after administration and has remarkable difference (P<0.05 or P<0.01) from that of the SHR model group, the pressure lowering action can be continued to 120 min after administration, and the blood pressure also has remarkable difference (P<0.05 or P<0.01) at 30 min, 60 min, 90 min and 120 min after administration compared with that before administration, referring to table 4.

(54) TABLE-US-00004 TABLE 4 Influence on caudal artery systolic pressure of spontaneously hypertensive rats caused by single medication in each drug group (x s, n = 8) Systolic pressure/mmHg (1 mmHg = 0.133322 kPa) Group Before administration 30 min 60 min 90 min 120 min Normal control 125.32 4.65 126.44 4.52 125.74 4.89 124.65 5.81 125.30 5.25 group Model control group 165.31 6.78** 163.56 5.65** 163.23 6.34** 164.69 6.75** 164.28 4.86** Positive control 161.24 11.03 149.75 8.09.sup..box-tangle-solidup..box-tangle-solidup.# 140.95 9.50.sup..box-tangle-solidup..box-tangle-solidup.## 139.23 6.04.sup..box-tangle-solidup..box-tangle-solidup.## 134.41 8.31.sup..box-tangle-solidup..box-tangle-solidup.## group GB high-dose 167.49 13.09 151.46 16.27.sup..box-tangle-solidup.# 157.54 17.95 160.26 16.54 152.21 18.27.sup..box-tangle-solidup.# group GB low-dose 168.41 12.30 160.42 9.56 153.15 12.51.sup..box-tangle-solidup.# 155.16 11.26.sup.# 159.17 11.63 group BA high-dose 163.64 10.31 149.69 11.62.sup..box-tangle-solidup..box-tangle-solidup.# 155.68 17.35 161.30 15.64 158.25 15.61 group BA low-dose 165.35 11.62 161.36 9.52 159.62 14.25 152.56 11.26.sup..box-tangle-solidup.# 159.87 11.32 group BD high-dose 164.57 13.25 148.47 10.87.sup..box-tangle-solidup..box-tangle-solidup.## 151.75 10.68.sup..box-tangle-solidup.# 157.25 14.38 160.49 14.76 group BD low-dose 163.64 14.02 157.74 9.28 152.67 13.52.sup..box-tangle-solidup.# 151.60 12.04.sup..box-tangle-solidup.# 155.68 10.15 group BZ high-dose 166.24 12.14 150.00 9.15.sup..box-tangle-solidup..box-tangle-solidup.## 152.95 9.26.sup..box-tangle-solidup.# 156.28 13.95 169.36 10.63 group BZ low-dose 163.58 10.36 152.65 10.48.sup..box-tangle-solidup.# 153.05 16.39 151.14 10.87.sup..box-tangle-solidup.# 156.47 12.47 group BL high-dose 164.25 11.69 148.01 5.98.sup..box-tangle-solidup..box-tangle-solidup.## 151.36 9.45.sup..box-tangle-solidup.# 152.95 12.54.sup..box-tangle-solidup.# 151.98 9.26.sup..box-tangle-solidup.# group BL low-dose 165.68 16.35 158.36 10.54 154.68 12.48 152.74 12.58.sup..box-tangle-solidup.# 152.04 10.02.sup..box-tangle-solidup.# group BM high-dose 163.45 15.24 149.04 10.95.sup..box-tangle-solidup..box-tangle-solidup.## 151.58 9.85.sup..box-tangle-solidup.# 153.65 11.04.sup..box-tangle-solidup.# 151.00 9.85.sup..box-tangle-solidup.# group BM low-dose 166.27 14.01 156.21 9.68 152.06 9.78.sup..box-tangle-solidup.# 151.74 13.15.sup..box-tangle-solidup.# 152.52 15.06.sup..box-tangle-solidup.# group Note: comparing the model control group with the normal control group, **P < 0.01; comparing each drug group with the model control group, .sup..box-tangle-solidup.P < 0.05, .sup..box-tangle-solidup..box-tangle-solidup.P < 0.01; and compared with pre-administration, .sup.#P < 0.05, .sup.##P < 0.01.

(55) 3.2.2 Influence on Caudal Artery Systolic Pressure of Spontaneously Hypertensive Rats Caused by Continuous Administration

(56) A test result shows that the blood pressure of spontaneously hypertensive rats gradually rises along with increase of age and has high-remarkable difference (P<0.01) compared with that of the normal control group, and from 7th day to 56th day of the test, the blood pressure has remarkable difference (P<0.05 or P<0.01) compared with that before the test; each drug group can remarkably inhibit a pathological progress of blood pressure rise of the spontaneously hypertensive rats, both the systolic pressure and diastolic pressure are lowered to some extent at different time periods after continuous medication, pressure lowering processes of low-dose groups of all the drug groups present certain fluctuation, pressure lowering processes of high-dose groups present a relatively stable pressure lowering effect, and the pressure lowering processes have remarkable difference (P<0.05 or P<0.01) compared with that of a model group and before medication, referring to table 5.

(57) TABLE-US-00005 TABLE 5 Influence on caudal artery systolic pressure of spontaneously hypertensive rats caused by 8 weeks of continuous medication in each drug group (x s, n = 8) Systolic pressure/mmHg (1 mmHg = 0.133322 kPa) Group Before administration 1 d 7 d 13 d 19 d Normal control 125.32 4.65 126.30 5.10 124.28 4.78 125.97 6.54 125.77 5.85 group Model 165.31 6.78** 164.85 6.24** 165.95 5.37** 164.88 8.24** 163.71 7.19** control group Positive control 161.24 11.03 145.17 6.42.sup..box-tangle-solidup..box-tangle-solidup.# 142.05 7.24.sup..box-tangle-solidup..box-tangle-solidup.## 140.36 9.20.sup..box-tangle-solidup..box-tangle-solidup.## 138.39 7.26.sup..box-tangle-solidup..box-tangle-solidup.## group GB high-dose 167.49 13.09 150.63 10.25.sup..box-tangle-solidup.# 148.54 9.27.sup..box-tangle-solidup.# 141.74 8.26.sup..box-tangle-solidup..box-tangle-solidup.## 140.95 12.04.sup..box-tangle-solidup..box-tangle-solidup.## group GB low-dose 168.41 12.30 155.67 12.36.sup.# 153.46 10.74.sup..box-tangle-solidup.# 156.25 10.84 150.68 9.78.sup..box-tangle-solidup.# group BA high-dose 163.64 10.31 151.48 9.85.sup..box-tangle-solidup.# 150.95 9.68.sup..box-tangle-solidup.# 145.30 11.62.sup..box-tangle-solidup..box-tangle-solidup.## 142.39 12.49.sup..box-tangle-solidup..box-tangle-solidup.## group BA low-dose 165.35 11.62 155.29 10.98.sup.# 158.73 13.82 153.64 9.75.sup..box-tangle-solidup.# 154.85 9.74.sup.# group BD high-dose 164.57 13.25 150.49 15.34.sup..box-tangle-solidup.# 152.64 11.45.sup..box-tangle-solidup.# 147.33 13.42.sup..box-tangle-solidup..box-tangle-solidup.## 145.49 12.98.sup..box-tangle-solidup..box-tangle-solidup.## group BD low-dose 163.64 14.02 156.20 13.41 152.95 11.03.sup..box-tangle-solidup.# 155.74 15.25 153.01 14.04.sup..box-tangle-solidup.# group BZ high-dose 166.24 12.14 154.69 9.85.sup..box-tangle-solidup.# 153.74 10.24.sup..box-tangle-solidup.# 150.39 10.39.sup..box-tangle-solidup.# 149.72 9.83.sup..box-tangle-solidup..box-tangle-solidup.## group BZ low-dose 163.58 10.36 158.77 11.06 153.41 14.08.sup..box-tangle-solidup.# 151.38 9.47.sup..box-tangle-solidup.# 155.74 10.62 group BL high-dose 164.25 11.69 153.56 8.15.sup..box-tangle-solidup.# 152.74 10.25.sup..box-tangle-solidup.# 149.52 10.24.sup..box-tangle-solidup..box-tangle-solidup.## 149.05 11.09.sup..box-tangle-solidup..box-tangle-solidup.## group BL low-dose 165.68 16.35 154.08 11.54.sup..box-tangle-solidup.# 156.92 9.82 156.07 8.52 153.96 11.07.sup..box-tangle-solidup.# group BM high-dose 163.45 15.24 154.62 5.74 152.31 10.56.sup..box-tangle-solidup.# 151.58 12.06.sup..box-tangle-solidup.# 153.06 10.41.sup..box-tangle-solidup.# group BM low-dose 166.27 14.01 155.25 12.36.sup.# 154.85 10.49.sup..box-tangle-solidup.# 153.26 12.95.sup..box-tangle-solidup.# 156.74 10.36 group Systolic pressure/mmHg (1 mmHg = 0.133322 kPa) Group 25 d 35 d 49 d 56 d Normal control 125.41 6.27 126.17 5.70 124.25 8.14 126.14 7.59 group Model control 164.76 8.04** 165.26 7.35** 164.96 7.28** 164.37 8.92** group Positive control 145.02 7.30.sup..box-tangle-solidup..box-tangle-solidup.# 139.08 6.72.sup..box-tangle-solidup..box-tangle-solidup.## 135.47 8.47.sup..box-tangle-solidup..box-tangle-solidup.## 130.39 4.65.sup..box-tangle-solidup..box-tangle-solidup.## group GB high-dose 147.46 9.85.sup..box-tangle-solidup..box-tangle-solidup.# 142.33 10.38.sup..box-tangle-solidup..box-tangle-solidup.## 138.79 11.58.sup..box-tangle-solidup..box-tangle-solidup.## 135.28 7.48.sup..box-tangle-solidup..box-tangle-solidup.## group GB low-dose 155.65 10.57.sup.# 154.25 10.74.sup..box-tangle-solidup.# 150.56 10.42.sup..box-tangle-solidup.## 153.85 9.24.sup..box-tangle-solidup.# group BA high-dose 145.84 11.46.sup..box-tangle-solidup.# 143.67 115.62.sup..box-tangle-solidup..box-tangle-solidup.## 140.69 14.72.sup..box-tangle-solidup..box-tangle-solidup.## 139.88 13.04.sup..box-tangle-solidup..box-tangle-solidup.## group BA low-dose 151.36 10.58.sup..box-tangle-solidup.# 153.72 13.68.sup..box-tangle-solidup.# 151.70 13.10.sup..box-tangle-solidup.# 153.72 12.54.sup..box-tangle-solidup.# group BD high-dose 147.66 14.02.sup..box-tangle-solidup..box-tangle-solidup.## 145.61 9.47.sup..box-tangle-solidup..box-tangle-solidup.## 148.62 12.04.sup..box-tangle-solidup..box-tangle-solidup.## 142.15 13.14.sup..box-tangle-solidup..box-tangle-solidup.## group BD low-dose 152.30 10.45.sup..box-tangle-solidup.# 157.03 14.08 153.96 14.74.sup..box-tangle-solidup.# 154.57 12.48.sup..box-tangle-solidup.# group BZ high-dose 149.02 8.26.sup..box-tangle-solidup..box-tangle-solidup.## 151.72 10.47.sup..box-tangle-solidup.# 153.42 12.04.sup..box-tangle-solidup.# 148.62 9.73.sup..box-tangle-solidup..box-tangle-solidup.## group BZ low-dose 154.94 9.64.sup..box-tangle-solidup.# 150.95 5.04.sup..box-tangle-solidup.# 155.06 6.37 153.95 14.01.sup..box-tangle-solidup.# group BL high-dose 147.09 12.68.sup..box-tangle-solidup..box-tangle-solidup.## 147.52 10.64.sup..box-tangle-solidup..box-tangle-solidup.## 150.94 11.06.sup..box-tangle-solidup.# 149.76 12.63.sup..box-tangle-solidup..box-tangle-solidup.## group BL low-dose 154.57 9.74.sup..box-tangle-solidup.# 153.67 11.58.sup..box-tangle-solidup.# 149.68 10.42.sup..box-tangle-solidup..box-tangle-solidup.## 153.62 9.47.sup..box-tangle-solidup.# group BM high-dose 148.54 11.62.sup..box-tangle-solidup..box-tangle-solidup.## 149.36 10.75.sup..box-tangle-solidup..box-tangle-solidup.## 150.32 9.59.sup..box-tangle-solidup.# 149.78 11.64.sup..box-tangle-solidup..box-tangle-solidup.## group BM low-dose 154.75 10.39.sup..box-tangle-solidup.# 151.48 10.32.sup..box-tangle-solidup.# 151.17 14.05.sup..box-tangle-solidup.# 154.64 11.42.sup..box-tangle-solidup.# group Note: comparing the model control group with the normal control group, **P < 0.01; comparing each drug group with the model control group, .sup..box-tangle-solidup.P < 0.05, .sup..box-tangle-solidup..box-tangle-solidup.P < 0.01; and compared with pre-administration, .sup.#P < 0.05, .sup.##P < 0.01.

(58) The above-mentioned test data show that high-dose groups and low-dose groups of the ginkgolide B derivative and the ginkgolide B can be used for remarkably lowering the blood pressure value of the spontaneously hypertensive rats after single and continuous administration, and thus, the ginkgolide B derivative and the ginkgolide B have a remarkable pressure lowering action.

(59) In summary, the ginkgolide B derivative and salt thereof, provided by the present invention, have obvious improved water solubility compared with that of ginkgolide B, and thus, the defects of the ginkgolide B that the water solubility is poor, the bioavailability is low, and the drug effect cannot be brought into full play are excellently overcome. A drug effect test shows that the compound provided by the present invention has a remarkable therapeutic action on cardiovascular and cerebrovascular diseases and can be applied to the prevention and/or hypertension, cerebral apoplexy, coronary diseases, arrhythmia, heart failure, dyslipidemia, pulmonary vascular diseases, chronic kidney diseases and peripheral vascular diseases, etc., such as myocardial infarction, coronary artery diseases, atherosclerosis, left main diseases, bifurcation lesions, angina pectoris, thrombosis, pulmonary heart diseases, endocrinopathy heart diseases, anemic heart diseases, cardiac neurosis, nutritional metabolic heart diseases, aortic aneurysm, lower extremity atherosclerotic diseases, peripheral arterial diseases, intracranial aneurysm, arteriosclerotic aneurysm, ischemic cerebral apoplexy, hemorrhagic cerebral apoplexy, hyperlipidemia, arteriosclerosis, exercise-related sudden death, sudden cardiac death, apoplexy, hypotension, vascular thrombosis, pulmonary embolism, atrial fibrillation, hypertensive encephalopathy, hypertension with cerebral stroke, cerebral hemorrhage, cerebral thrombosis, cerebral embolism, cerebral infarction, cerebral arteritis, cerebral arteriosclerosis, lacunar infarction, vascular dementia, chronic kidney diseases, chronic cardiac insufficiency, gouty nephropathy, diabetic nephropathy and/or abnormal renal function, and thus, a new choice is provided for clinically preventing and/or treating the cardiovascular and cerebrovascular diseases.