COMPOSITIONS AND METHODS FOR HAIR AND SCALP TREATMENT

Abstract

The present invention provides a mixture for the use of synergic treating scalp and hair-disorders, the mixture comprises at least one first compound having premixing specific rotation value, Formula I, peaks within RTs 8.23-8.82 min., at least one second compound, having a Formula II, and RTs 12.67, 12.84, 13.14 and 18.14 min., and at least one third compound having a Formula III and RTs 11.52, 11.59, 15.53, 17.21 and 18.15 min; wherein the mixture is characterized by post mixture specific rotation value Formula IV and by GCMS first, second and third peaks of retention time 11.55, 11.94 and 12.69 min., respectfully; wherein the mixture is characterized by the first peak (11.55 min) to the second peak (11.94 min) ratio between 1:7 to 1:2; the second peak to the third peak (12.69 min.) ratio between 1:4 to 3:2; and the first peak to the third peak ratio of between 1:12 to 1:3 as determined by GCMS peak areas; and further wherein the GCMS is Model GCD 1800, utilized under the following conditions: preparation for analysis was performed by basic extraction (pH 10) in dichloromethane, 3 times, ratio of 1 to 5 sample to solvent; acid extraction (pH 3) in dichloromethane, 3 times, ratio of 1 to 5 sample to solvent; consolidation of the extracts and evaporating in vacuum to a volume of 10 ml; injection of 2 microliters of the sample into the GCMS, using the following program: 60 degrees C. for 7 minutes, increasing at 15 degrees C. per minute to 320 degrees C., staying at 320 degrees C. for 15 minutes.

Claims

1. A synergic water based herbal mixture for the use in the treatment of scalp and hair-disorders of a mammalian subject, comprising at least three herbs, wherein first herb is at least one of Urtica diocia or Pipturus albidus, having premixing specific rotation value (PSRV) [].sub.D.sup.20=99 and GCMS 6 peaks within RTs 8.23-8.82 min., second herb is at least one of Thymus vulgaris or Salvia officinalis having a PSRV [].sub.D.sup.20=+33 and RTs 12.67, 12.84, 13.14 and 18.14 min., and third herb is at least one of Rosmarinus officinalis or Mentha viridis having a PSRV [].sub.D.sup.20=5 and RTs 11.52, 11.59, 15.53, 17.21 and 18.15 min; wherein said mixture is characterized by post mixture specific rotation value [].sub.D.sup.20=+18 and by GCMS first, second and third peaks of retention time 11.55, 11.94 and 12.69 min., respectively; wherein said mixture is characterized by said first peak (11.55 min) to said second peak (11.94 min) ratio between 1:7 to 1:2; said second peak to said third peak (12.69 min.) ratio between 1:4 to 3:2; and said first peak to said third peak ratio of between 1:12 to 1:3 as determined by GCMS peak areas; and further wherein said GCMS is Model GCD 1800, utilized under the following conditions: preparation for analysis was performed by basic extraction (pH=10) in dichloromethane, 3 times, ratio of 1 to 5 sample to solvent; acid extraction (pH=3) in dichloromethane, 3 times, ratio of 1 to 5 sample to solvent; consolidation of the extracts and evaporating in vacuum to a volume of 10 ml; injection of 2 microliters of the sample into the GCMS, using the following program: 60 degrees C. for 7 minutes, increasing at 15 degrees C. per minute to 320 degrees C., staying at 320 degrees C. for 15 minutes.

2. The mixture of claim 1, wherein first peak area of first peak to second peak to third peak ratio is from 5:17:45 and 5:8:18.

3. The mixture of claim 1, wherein said mixture comprises at least one compound selected from the group consisting of water, ethanol, anti-bacterial agents, moisturizers, hydration agents, penetration agents, preservatives, viscosity-controlling agents anesthetics, vitamins, anti-itch actives, botanical extracts, color agents, enzymes, conditioning agents, darkening or lightening agents, nutrients, micronutrients, fragrances, glitters, hair pigment additives, humectants, mica, minerals, oils, polyphenols, silicones or derivatives thereof, sun-blocks, surfactants, vitamins, waxes, solvents, rheology modifiers, suspending agent, thickeners, hair health actives, anti-dandruff mixtures, anti-oxidants, pigments, abrasives, absorbents, biological actives, buffering agents, chelating agents, opacifying agents, pH adjusters, chelates, perfumes, brighteners, sensates, attractants, dyes, bleaches and any combination thereof.

4. The mixture of claim 1, wherein said mixture is provided in a form selected from a group consisting of liquid, solution, capsule, gel, dissolving capsule, capsule comprising a filter, infusion, cream, ointment, electroporation formulation, foam, gel, suspension, emulsion, lotion, microspheres, microbeads, mud, oil, paste, ointment, patch, powder, roller stick, soap, surfactant, spray, shampoo conditioner, a rinse-off conditioner, a tablet, a pill or a granulated powder, nutraceutical, a functional food, a dietary supplement, a food supplement, a pharmaceutical product or a cosmetic product topical, subcutaneous, dermal, oral, and any combination thereof.

5. The mixture according to claim 1, wherein said treatment comprises: applying said mixture on said mammalian head, and massaging said mixture in scalp of said mammalian subject.

6. The mixture according to claim 1, wherein said mammalian subject is selected from a group consisting of humans, monkeys, cats, cows, dogs, gerbils, goats, guinea pigs, hamsters, horses, mice, prairie dogs, rabbits, rats, sheep and squirrels.

7. The mixture according to claim 1, wherein said treatment is selected from a group consisting of inducing new hair growth, stimulating new hair growth, increasing hair growth, preventing hair regression, cleansing the hair, cleansing the scalp, beautifying the hair, promoting attraction, altering appearance of hair, and any combination thereof.

8. The mixture according to claim 1, wherein said mixture is produced by a step of admixing said mixtures with at least one member of a group consisting of water, ethanol, one or more anti-bacterial agents, moisturizers and hydration agents, penetration agents, preservatives, viscosity-controlling agents anesthetics, vitamins, anti-itch actives, botanical extracts, color agents, enzymes, conditioning agents, darkening or lightening agents, fragrances, glitters, hair pigment additives, humectants, mica, minerals, oils, polyphenols, silicones or derivatives thereof, sun-blockers, surfactants, vitamins, micronutrients, nutrients, waxes, solvents, rheology modifiers, suspending agents, thickeners, hair health actives, anti-dandruff actives, anti-oxidants, pigments, abrasives, absorbents, biological actives, buffering agents, chelating agents, opacifying agents, pH adjusters, chelates, perfumes, brighteners, sensates, attractants, dyes, bleaches and any combination thereof.

9. The mixture according to claim 1, wherein said mixture is further produced, in the form selected from a group consisting of liquid, solution, capsule, gel, dissolving capsule, capsule comprising of filter, infusion, cream, ointment, electroporation formulation, emulsion, foam, gel, suspension, emulsion, lotion, microspheres, microbeads, mud, oil, paste, ointment, patch, powder, roller stick, soap, surfactant, spray, shampoo conditioner, a rinse-off conditioner, and any combination thereof.

10. The mixture according to claim 1, wherein said scalp and hair-disorders of a mammalian subject are selected from the group consisting of alopecia, accidental injury, damage to hair follicles, surgical trauma, burn wound, radiation or chemotherapy treatment site, incisional wound, donor site wound from skin transplant and ulcer; a desire to modify physical appearance, and any combination thereof; or to enhance one or more hair properties selected from the group consisting of brilliance, fullness, gloss, glow, length, luster, patina, sheen, shine, thickness, volume and any combination thereof.

11. A kit for providing a mixture for use in the treatment of hair or scalp disorders in a mammalian subject , inducing or stimulating new hair growth, increasing hair growth or preventing hair regression in a mammalian subject, cleansing the hair, cleansing the scalp, beautifying the hair, promoting attraction and altering the appearance of hair and any combination thereof said kit comprising: a synergic water based herbal mixture comprising at least three herbs, wherein first herb is at least one of Urtica diocia or Pipturus albidus having premixing specific rotation value (PSRV) [].sub.D.sup.20=99 and GCMS 6 peaks within RTs 8.23-8.82 min., second herb is at least one of Thymus vulgaris or Salvia officinalis having a PSRV [].sub.D.sup.20=+33 and RTs 12.67, 12.84, 13.14 and 18.14 min., and third herb is at least one of Rosmarinus officinalis or Mentha viridis having a PSRV [].sub.D.sup.20=5 and RTs 11.52, 11.59, 15.53, 17.21 and 18.15 min; wherein said mixture is characterized by post mixture specific rotation value [].sub.D.sup.20=+18 and by GCMS first, second and third peaks of retention time 11.55, 11.94 and 12.69 min., respectively; wherein said mixture is characterized by said first peak (11.55 min) to said second peak (11.94 min) ratio between 1:7 to 1:2; said second peak to said third peak (12.69 min.) ratio between 1:4 to 3:2; and said first peak to said third peak ratio of between 1:12 to 1:3 as determined by GCMS peak areas; and further wherein said GCMS is Model GCD 1800, utilized under the following conditions: preparation for analysis was performed by basic extraction (pH=10) in dichloromethane, 3 times, ratio of 1 to 5 sample to solvent; acid extraction (pH=3) in dichloromethane, 3 times, ratio of 1 to 5 sample to solvent; consolidation of the extracts and evaporating in vacuum to a volume of 10 ml; injection of 2 microliters of the sample into the GCMS, using the following program: 60 degrees C. for 7 minutes, increasing at 15 degrees C. per minute to 320 degrees C., staying at 320 degrees C. for 15 minutes.

12. The kit of claim 11, wherein peak area of first peak to second peak to third peak ratio is from 5:17:45 to 5:8:18.

13. The kit of claim 11, wherein said application means is selected from the group consisting of, brushes, combs, controlled-release matrices, fabrics, pumps, sprayers, especially aerosol spray dispensers, self-pressurized spray dispensers and non-aerosol spray dispensers, sachets, tea bags, paper tea bags, corn-made tea bags, fabric tea bags, pouches, bags, aluminum bags, cloth bags, plastic bags, filtered bottles, bottles with stainless steel filters, bottles with plastic filters, stainless steel filters, plastic filters, droppers, capsules, balls, compressed cubes, filter, corn paper filters, a filter cloths, organic filters, paper filters, ampoules, pouches, flasks, rollers, plastic rollers, plastic rollers and stainless steel, wands, or a container selected from the group consisting of bottles, cans, tubes, vials, dispensers, sachets, tea bags, paper tea bags, corn-made tea bags, fabric tea bags, pouches, bags, aluminum bags, cloth bags, plastic bags, filtered bottles, bottles with stainless steel filters, bottles with plastic filters, and any combination thereof.

14. The kit of claim 11, wherein said mixture further comprises at least one member of a group consisting of water, ethanol, anti-bacterial agents, moisturizers and hydration agents, penetration agents, preservatives, viscosity-controlling agents anesthetics, vitamins, anti-itch actives, botanical extracts, color agents, enzymes, conditioning agents, darkening or lightening agents, nutrient, micronutrient, fragrance, glitter, hair pigment additives, humectants, mica, minerals, oils, polyphenols, silicones or derivatives thereof, sun-blocks, surfactants, vitamins, waxes, solvents, rheology modifiers, suspending agent, thickeners, hair health actives, anti-dandruff actives, anti-oxidants, pigments, abrasives, absorbents, biological actives, buffering agents, chelating agents, opacifying agents, pH adjusters, chelates, perfumes, brighteners, sensates, attractants, dyes, bleaches and any combination thereof.

15. The kit of claim 11, wherein said mixture is provided in the form selected from a group consisting of liquid, solution, capsule, gel, dissolving capsule, capsule comprising of filter, infusion, cream, ointment, electroporation formulation, emulsion, foam, gel, suspension, emulsion, lotion, microspheres, microbeads, mud, oil, paste, ointment, patch, powder, roller stick, soap, surfactant, spray, shampoo conditioner, a rinse-off conditioner, and any combination thereof.

16. The kit of claim 11, wherein said mixture is configured to be administrable in a manner selected from a group consisting of topical, subcutaneous, dermal, oral, tablet, a pill or a granulated powder, nutraceutical, a functional food, a dietary supplement, a food supplement, a pharmaceutical product or a cosmetic product and any combination thereof.

17. The kit of claim 11, wherein said hair or scalp disorders are selected from the group consisting of alopecia, accidental injury, damage to hair follicles, surgical trauma, burn wound, radiation or chemotherapy treatment site, incisional wound, donor site wound from skin transplant and ulcer; a desire to modify physical appearance, or any combination thereof.

18. The kit of claim 11, wherein said mixture configured to enhance one or more hair properties selected from the group consisting of brilliance, fullness, gloss, glow, length, luster, patina, sheen, shine, thickness, volume and any combination thereof.

19. A method for preparation of a synergic water based herbal mixture for scalp or hair treatment, wherein said method comprises steps of: mixing at least three herbs, wherein first herb is at least one of Urtica diocia or Pipturus albidus having premixing specific rotation value (PSRV) [].sub.D.sup.20=99 and GCMS 6 peaks within RTs 8.23-8.82 min., second herb is at least one of Thymus vulgaris or Salvia officinalis having a PSRV [].sub.D.sup.20=+33 and RTs 12.67, 12.84, 13.14 and 18.14 min., and third herb is at least one of Rosmarinus officinalis or Mentha viridis having a PSRV [].sub.D.sup.20=5 and RTs 11.52, 11.59, 15.53, 17.21 and 18.15 min.; thereby producing a mixture; and immersing said mixture in boiling water for 10 to 180 minutes; wherein said mixture is characterized by post mixture specific rotation value [].sub.D.sup.20=+18 and by GCMS first, second and third peaks of retention time 11.55, 11.94 and 12.69 min., wherein said mixture is characterized by said first peak (11.55 min) to said second peak (11.94 min) ratio between 1:7 to 1:2; said second peak to said third peak (12.69 min.) ratio between 1:4 to 3:2; and said first peak to said third peak ratio of between 1:12 to 1:3 as determined by GC peak areas; and further wherein utilizing GCMS, Model GCD 1800 under the following conditions: preparation for analysis was performed by basic extraction (pH=10) in dichloromethane, 3 times, ratio of 1 to 5 sample to solvent; acid extraction (pH=3) in dichloromethane, 3 times, ratio of 1 to 5 sample to solvent; consolidation of the extracts and evaporating in vacuum to a volume of 10 ml; injection of 2 microliters of the sample into the GCMS, using the following program: 60 degrees C. for 7 minutes, increasing at 15 C per minute to 320 degrees C., staying at 320 degrees C. for 15 minutes.

20. The method according to claim 19, wherein said step of immersing is followed by boiling said mixture and then cooking the boiled mixture for additional up to 60 minutes in 40 C. to 80 C.

Description

BRIEF DESCRIPTION OF THE FIGURES

[0085] The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention.

[0086] FIG. 1 Photos of a female (Female #1), treated with the composition of the current invention. Photos (A) were taken 5 hours before shampooing compared to photos taken 5 hours following 18 times of shampooing (B), of the same female individual. Photos were taken in a back view (A1, B1), a side view (A2, B2), and from an overhead view (A3, B3);

[0087] FIG. 2a Micrographs of hair of Female #1, taken under a light microscope at a 25-fold magnification. Hairs A and B were taken from an individual before use of the composition, while hairs C and D were taken following treatment of said composition for about a month. The micrographs were taken by Kaps Som 62 dental microscope with Sony Next camera connected by TV tube.

[0088] FIG. 2b Micrographs of hair of Female #1, taken under a light microscope at a 100-fold magnification. Hairs A and B were taken from an individual before use of the composition, while hairs C and D were taken following treatment of said composition for 18 times of shampooing, about a month.

[0089] The micrographs were taken by light microscope Olympus B.H-2, connected to an Olympus camera, using DP70 software.

[0090] FIG. 2c Micrographs of hair of Female #1, taken under a light microscope at a 200-fold magnification. Hairs A and B were taken from an individual before use of the composition, while hairs C and D were taken following treatment of said composition 18 times of shampooing, about a month.

[0091] The micrographs were taken by light microscope Olympus B.H-2, connected to an Olympus camera, using DP70 software.

[0092] FIG. 2d Micrographs of hair of Female #1, taken under a light microscope at a 400-fold magnification. Hairs A and B were taken from an individual before use of the composition, while hairs C and D were taken following treatment of said composition 18 times of shampooing, about a month.

[0093] The micrographs were taken by light microscope Olympus B.H-2, connected to an Olympus camera, using DP70 software.

[0094] FIG. 3 Micrographs of hair of Female #2, taken under a light microscope at a 100-fold (A), a 200-fold (B), a 400-fold (C) magnification. Hairs A and B and C were taken from an individual following treatment of said composition for about seven months.

[0095] The micrographs were taken by light microscope Olympus B.H-2, connected to an Olympus camera, using DP70 software.

[0096] FIG. 4 A GC-MS chromatogram of the mixture according to one embodiment of the invention.

[0097] FIGS. 5A and 5B A GC-MS chromatogram of the first compound according to one embodiment of the invention.

[0098] FIG. 6 A GC-MS chromatogram of the second compound according to one embodiment of the invention.

[0099] FIG. 7 A GC-MS chromatogram of the third compound according to one embodiment of the invention.

[0100] FIG. 8A-FIG. 8C A kit according to one to one embodiment of the invention.

[0101] FIG. 9 A map of hair location on the scalp.

[0102] FIG. 10 An electron microscope micrograph of hair the vertex area, of Female #3.

[0103] FIG. 11 An electron microscope micrograph of hair the rear area, of Female #3.

[0104] FIG. 12 An electron microscope micrograph of hair the front area, of Female #3.

[0105] FIG. 13 An electron microscope micrograph of hair the parietal area, of Female #3.

[0106] FIG. 14 An electron microscope micrograph of hair the nape area, of Female #3.

[0107] FIG. 15 An electron microscope micrograph of hair the temporal area, of Female #3.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0108] Reference will now be made in detail to the preferred embodiments of the present invention, examples of which are illustrated in the accompanying drawings. The current invention discloses, in a non-limiting manner, a hair and scalp treating, water based herbal mixture for the use of synergic treating scalp and hair-disorders (hereinafter mixture) which comprises, inter alia, at least one first herb having premixing specific rotation value (PSRV) [].sub.D.sup.20=99 and GCMS 6 peaks within RTs 8.23-8.82 min., at least one second herb, having a PSRV [].sub.D.sup.20=+33 and RTs 12.67, 12.84, 13.14 and 18.14 min., and at least one third herb having a PSRV [].sub.D.sup.20=5 and RTs 11.52, 11.59, 15.53, 17.21 and 18.15 min; wherein said mixture is characterized by post mixture specific rotation value [].sub.D.sup.20=+18 and by GCMS first, second and third peaks of retention time 11.55, 11.94 and 12.69 min., respectively; wherein said mixture is characterized by said first peak (11.55 min) to said second peak (11.94 min) ratio between 1:7 to 1:2; said second peak to said third peak (12.69 min.) ratio between 1:4 to 3:2; and said first peak to said third peak ratio of between 1:12 to 1:3 as determined by GCMS peak areas; and further wherein said GCMS is Model GCD 1800, utilized under the following conditions: preparation for analysis was performed by basic extraction (pH=10) in dichloromethane, 3 times, ratio of 1 to 5 sample to solvent; acid extraction (pH=3) in dichloromethane, 3 times, ratio of 1 to 5 sample to solvent; consolidation of the extracts and evaporating in vacuum to a volume of 10 ml; injection of 2 microliters of the sample into the GCMS, using the following program: 60 degrees C. for 7 minutes, increasing at 15 degrees C. per minute to 320 degrees C., staying at 320 degrees C. for 15 minutes.

[0109] The term about refers to any value being up to 20% lower or greater the defined measure. GC conditions are defined below.

[0110] The current invention also discloses aforesaid hair and scalp treating mixture, wherein peaks area of Group A to Group B to Group C ratio is about (3-7):(10-22):(30-60) in general, and more specifically, about 5:17:45.

[0111] It is in the scope of the invention wherein materials of the first compound (GROUP A) are one or more materials selected from a group consisting of 2-Borneol or Bingpian or (1R,2S,4R)-rel-1,7,7-TriMethylbicyclo[2.2.1]heptan-2-ol or Bornel (crystal) or Endo-1,7,7-trimethyl-bicyclo[2.2.1]heptan-2-ol 2-Camphanol or 1,7,7-Trimethylbicyclo(2.2.1)heptan-2-ol or endo-; 1,7,7-trimethyl-endo-Bicyclo[2.2.1]heptan-2-ol or 2-Bornanol, endo- or 507-70-0 OR (1S-endo)-1,7,7-Trimethylbicyclo(2.2.1)heptan-2-ol Or 000464-45-9 or ()-Borneol or 1-Bornyl alcohol; Borneol, (1S,2R,4S)-()-; 1-2-Bornanol or 1-2-Camphanol; 1-Borneol; L-Borneol or Linderol or NGAI camphor or Bicyclo(2.2.1)heptan-2-ol, 1,7,7-trimethyl-, (1S,2R,4S)- OR 1192-62-7 or 2-Acetylfuran or 1-(2-furanyl)ethanone or Furan, 2-acetyl-; acetylfuran; Ethanone, 1-(2-furanyl)- or 2-Furyl methyl ketone; 2-Acetylfurane or 1-(2-furanyl)ethanone (acetylfuran) or (2-furanyl)-1-ethanone or 1-(2-Furanyl)-ethanone (2-acetylfuran) or furyl methyl ketone or 1-(2-Furyl)ethanone or Ketone, 2-furyl methyl; Methyl 2-furyl ketone.

[0112] It is also in the scope of the invention wherein materials of the second compound (GROUP B) are one or more materials selected from a group consisting of 2-PINEN-4-ONE or 4,6,6-TRIMETHYLBICYCLO[3.1.1]HEPT-3-EN-2-ONE or VERBENONE or 4,6,6-trimethyl-bicyclo[3.1.1]hept-3-en-2-on or Berbenone or 2-Pinen-4-one, 4,6,6- or Trimethylbicyclo[3.1.1]hept-3-en-2-one or 2,6,6-Trimethylbicyclo[3.1.1]hepta-2-ene-4-one or Pina-2-ene-4-one or 80-57-9 OR 503-93-5 OR Eucarvone OR 2,6,6-Trimethyl-2,4-cycloheptadien-1-one OR 2,4-cycloheptadien-1-one, 2,6,6-trimethyl- OR 2,6,6-trimethyl-2,4-cycloheptadien-1-one OR 2,6,6-trimethylcyclohepta-2,4-dien-1-one OR 2,4-cycloheptadien-1-one-2,6,6-trimethyl OR 73657-70-2 OR 2-Pyridinecarbonitrile,1,2,5,6-tetrahydro-1,3,4-trimethyl- OR NSC 375118 OR 2-Pyridinecarbonitrile, 1,2,5,6-tetrahydro-1,3,4-trimethyl- or 1,4,5-trimethyl-3,6-dihydro-2H-pyridine-6-carbonitrile OR 1,4,5-trimethyl-3,6-dihydro-2H-pyridine-6-carbonitrile.

[0113] It is yet also in the scope of the invention wherein materials of the third compound (GROUP C) are one or more materials selected from a group consisting of 1-Hydroxy-2-methyl-5-isopropylbenzene or 2-Hydroxy-1-methyl-4-(1-methylethyl)benzene; 2-hydroxy-4-(2-propyl)toluene or 2-hydroxy-p-cymen or 2-methyl-5-(1-methylethyl)-pheno or 2-para-cymenol; 2-p-Cymenol or 3-isopropyl-6-methyl-pheno or Carvacrol Or 499-75-2 OR 26967-65-7 OR Phenol, diethyl- OR Diethylphenol OR 2,3-diethylphenol; 2,5-diethylphenol; 3,5-diethylphenol Phenol, diethyl- OR Diethylphenol OR 89-83-8 OR Thymol OR p-Cymen-3-ol OR 2-Isopropyl-5-methylphenol; m-Thymol OR 2-Isopropyl-5-methylphenol (thymol); Isopropyl cresol OR 5-Methyl-2-(1-methylethyl)-phenol OR Phenol, 5-methyl-2-(1-methylethyl)- OR Thyme camphor OR 3-Hydroxy-p-cymene OR 3 -Methyl-6-isopropyl phenol OR 5-Methyl-2-isopropyl phenol OR 6-Isopropyl-m-cresol OR 6-Isopropyl-3-methylphenol OR m-Cresol, 6-isopropyl- OR p-Cymene, 3-hydroxy- OR Phenol, 2-isopropyl-5-methyl- OR Thymic acid OR 1-Hydroxy-5-methyl-2-isopropylbenzene OR 1-Methyl-3-hydroxy-4-isopropylbenzene OR 3-p-Cymenol OR 3-Hydroxy-1-methyl-4-isopropylbenzene OR 5-Methyl-2-isopropyl-1-phenol OR Isopropyl-m-cresol OR 2-Hydroxy-1-isopropyl-4-methylbenzene OR NSC 11215.

[0114] The present invention also discloses hair and scalp treating mixtures as defined in any of the above, wherein the mixture further comprises at least one member of a group consisting, inter alia, water, water-miscible alcohol such as ethanol, anti-bacterial agents, moisturizers and hydration agents, penetration agents, preservatives, viscosity-controlling agents anesthetics, vitamins, anti-itch actives, botanical extracts, color agents, enzymes, conditioning agents, darkening or lightening agents, nutrient, micronutrient, fragrance, glitter, hair pigment additives, humectants, mica, minerals, oils, polyphenols, silicones or derivatives thereof, sunblocks, surfactants, vitamins, waxes, solvents, rheology modifiers, suspending agent, thickeners, hair health actives, anti-dandruff actives, anti-oxidants, pigments, abrasives, absorbents, biological actives, buffering agents, chelating agents, opacifying agents, pH adjusters, chelates, perfumes, brighteners, sensates, attractants, dyes, bleaches and any combination thereof.

[0115] The present invention also discloses hair and scalp treating mixtures as defined in any one of the above, wherein the mixture comprises at least one preservative, inter alia a preservative named Geogard Ultra, comprising gluconolactone, calcium gluconate, sodium benzoate and sodium bicarbonate.

[0116] The present invention also discloses hair and scalp treating mixtures as defined in any of the above, wherein the mixture is provided in a form selected from a group consisting, inter alia, liquid, solution, capsule, gel, dissolving capsule, capsule comprising of filter, infusion, cream, ointment, electroporation formulation, emulsion, foam, gel, suspension, emulsion, lotion, microspheres, microbeads, mud, oil, paste, ointment, patch, powder, roller stick, soap, surfactant, spray, shampoo conditioner, a rinse-off conditioner, and any combination thereof.

[0117] The present invention also discloses hair and scalp treating mixtures as defined in any of the above, wherein the mixture is configured to be administrable in a manner selected from a group consisting, inter alia, a group comprising topical, subcutaneous, dermal, oral, tablet, a pill or a granulated powder, nutraceutical, a functional food, a dietary supplement, a food supplement, a pharmaceutical product or a cosmetic product and any combination thereof.

[0118] The present invention also discloses a method for treating hair and scalp of a mammalian subject. The method comprises, inter alia, steps of: obtaining a mixture comprising at least one compound selected from Group A having a GC retention time 11.55 min.; at least one compound selected from Group B having a GC retention time of 11.94 min.; and at least one compound selected from Group C having a GC retention time of 12.69 min.; wherein GC peak area of Group A to Group B ratio is ranging from about 1:7 to about 1:2; peak area of Group B to Group C ratio is ranging from about 1:4 to about 3:2; and peak area of Group A to Group C ratio is ranging from about 1:12 to 1:3. It is according to a specific embodiment of the invention wherein peaks area of Group A to Group B to Group C ratio is about (3-7):(10-22):(30-60) in general, and more specifically, about 5:17:45.

[0119] It is in the scope of the invention wherein aforesaid method further comprising step(s) selected from step (a) applying said mixture on said mammalian head, and step (b) massaging the mixture in scalp of the mammalian subject.

[0120] The term mammalian refers hereinafter to any member of a group consisting of humans, including males, females, toddlers and grown up persons, and other mammals such as monkeys, pets e.g., cats, cows, dogs, gerbils, goats, guinea pigs, hamsters, farm animals, e.g., horses, mice, prairie dogs, rabbits, rats, sheep, squirrels and a combination thereof.

[0121] It is in the scope of the invention wherein the method as defined above is provided useful for a treatment selected from a group consisting, inter alia, inducing and/or stimulating new hair growth, increasing hair growth or preventing hair regression, cleansing the hair, cleansing the scalp, beautifying the hair promoting attraction, altering the appearance of hair and any combination thereof.

[0122] It is also in the scope of the invention wherein the method as defined in any of the above is provided useful for producing a mixture by a step or set of steps of admixing mixtures with at least one material or diluent selected from a group consisting, inter alia, water, water miscible alcohol such as ethanol, one or more anti-bacterial agents, moisturizers and hydration agents, penetration agents, preservatives, viscosity-controlling agents anesthetics, vitamins, anti-itch actives, botanical extracts, color agents, enzymes, conditioning agents, darkening or lightening agents, fragrance, glitter, hair pigment additives, humectants, mica, minerals, oils, polyphenols, silicones or derivatives thereof, sun-blockers, surfactants, vitamins, micronutrients, nutrients, waxes, solvents, rheology modifiers, suspending agent, thickeners, hair health actives, anti-dandruff actives, anti-oxidants, pigments, abrasives, absorbents, biological actives, buffering agents, chelating agents, opacifying agents, pH adjusters, chelates, perfumes, brighteners, sensates, attractants, dyes, bleaches and any combination thereof.

[0123] It is also in the scope of the invention wherein the method as defined in any of the above comprises step(s) of providing said mixture in the form selected from a group consisting, inter alia, liquid, solution, capsule, gel, dissolving capsule, capsule comprising of filter, infusion, cream, ointment, electroporation formulation, emulsion, foam, gel, suspension, emulsion, lotion, microspheres, microbeads, mud, oil, paste, ointment, patch, powder, roller stick, soap, surfactant, spray, shampoo conditioner, a rinse-off conditioner, and any combination thereof.

[0124] It is also in the scope of the invention wherein the method as defined in any of the above comprising step(s) of administrating the mixture in a manner selected of a group consisting, inter alia, topically, subcutaneously, dermally, orally, and any combination thereof.

[0125] It is also in the scope of the invention wherein the method as defined in any of the above comprising step(s) of administrating said mixture in a manner selected of a group consisting of a tablet, a pill or a granulated powder, a nutraceutical, a functional food, a dietary supplement, a food supplement, a pharmaceutical product and a cosmetic product.

[0126] It is also in the scope of the invention wherein the method as defined in any of the above is provided useful for treating a condition selected from the group consisting, inter alia, alopecia, accidental injury, damage to hair follicles, surgical trauma, burn wound, radiation or chemotherapy treatment site, incisional wound, donor site wound from skin transplant and ulcer; a desire to modify physical appearance, and any combination thereof; or to enhance one or more hair properties selected from the group consisting of brilliance, fullness, gloss, glow, length, luster, patina, sheen, shine, thickness, volume and any combination thereof.

[0127] The invention further discloses a kit for either inducing or stimulating new hair growth, increasing hair growth or preventing hair regression in a mammalian subject, cleansing the hair, cleansing the scalp, beautifying the hair, promoting attraction and altering the appearance of hair. This kit comprises, inter alia, a mixture comprising at least one compound selected from Group A having a GC retention time 11.55 min.; at least one compound selected from Group B having a GC retention time of 11.94 min.; and at least one compound selected from Group C having a GC retention time of 12.69 min.; wherein GC peak area of Group A to Group B ratio is ranging from about 1:7 to about 1:2; peak area of Group B to Group C ratio is ranging from about 1:4 to about 3:2; and peak area of Group A to Group C ratio is ranging from about 1:12 to 1:3. It is according to a specific embodiment of the invention wherein peaks area of Group A to Group B to Group C ratio of the mixture in the kit is about (3-7):(10-22):(30-60) in general, and more specifically, about 5:17:45.

[0128] It is in the scope of the invention wherein the aforesaid kit comprises a mixture which is contained in a vessel. The vessel is selected from the group consisting, inter alia, containers, bottles, brushes, cans, combs, controlled-release matrices, fabrics, pumps, sprayers, especially aerosol spray dispensers, self-pressurized spray dispensers and non-aerosol spray dispensers, tubes, vials, a tea bag, a paper tea bad, a corn-made tea bag, a fabric tea bag, a dye, a pouch, a bag, an aluminum bag, a cloth bag, a plastic bag, a bottle, a filtered bottle, a bottle with stainless steel filter, a bottle with a plastic filter, a stainless steel filter, a plastic filter, a dropper, capsules, balls, compressed cubes, a filter, a corn paper filter, a filter cloth, an organic filter, a paper filter, an ampoule, a pouch, a flask, a roller, a plastic roller, a flask, a plastic roller and stainless steel, wands and combination thereof.

[0129] It is also in the scope of the invention wherein the kit as defined in any of the above comprising one or more mixtures, at least one mixture comprises or diluted with at least one member of a group consisting, inter alia, water, water-miscible alcohol such as ethanol, anti-bacterial agents, moisturizers and hydration agents, penetration agents, preservatives, viscosity-controlling agents anesthetics, vitamins, anti-itch actives, botanical extracts, color agents, enzymes, conditioning agents, darkening or lightening agents, nutrient, micronutrient, fragrance, glitter, hair pigment additives, humectants, mica, minerals, oils, polyphenols, silicones or derivatives thereof, sun-blocks, surfactants, vitamins, waxes, solvents, rheology modifiers, suspending agent, thickeners, hair health actives, anti-dandruff actives, anti-oxidants, pigments, abrasives, absorbents, biological actives, buffering agents, chelating agents, opacifying agents, pH adjusters, chelates, perfumes, brighteners, sensates, attractants, dyes, bleaches and any combination thereof.

[0130] It is also in the scope of the invention wherein the kit as defined in any of the above comprising a mixture which is provided in a form selected from a group consisting, inter alia, liquid, solution, capsule, gel, dissolving capsule, capsule comprising of filter, infusion, cream, ointment, electroporation formulation, emulsion, foam, gel, suspension, emulsion, lotion, microspheres, microbeads, mud, oil, paste, ointment, patch, powder, roller stick, soap, surfactant, spray, shampoo conditioner, a rinse-off conditioner, and any combination thereof.

[0131] It is also in the scope of the invention wherein the kit as defined in any of the above comprising a mixture which is configured to be administrable in a manner selected from a group consisting, inter alia, a group comprising topical, subcutaneous, dermal, oral, tablet, a pill or a granulated powder, nutraceutical, a functional food, a dietary supplement, a food supplement, a pharmaceutical product or a cosmetic product and any combination thereof.

[0132] It is also in the scope of the invention wherein the kit as defined in any of the above comprising a mixture configured to treat a mammalian subject which has a condition or conditions. The condition to be treated are selected from the group consisting, inter alia, of alopecia, accidental injury, damage to hair follicles, surgical trauma, burn wound, radiation or chemotherapy treatment site, incisional wound, donor site wound from skin transplant and ulcer; a desire to modify physical appearance, or any combination thereof.

[0133] It is also in the scope of the invention wherein the kit as defined in any of the above comprising a mixture configured to enhance one or more hair properties selected from the group consisting of brilliance, fullness, gloss, glow, length, luster, patina, sheen, shine, thickness, volume and any combination thereof.

[0134] According to one embodiment of the invention, mixture(s) of the current invention is/are a mixture of herbs, prepared as either infusion (following soaking in boiled water), a cooked product-after cooking in medium temperate, vapors or being provided as an extract, using either organic or inorganic solvents.

[0135] The term herbs refers hereinafter to one or more herbs, spices, plants, parts, mixtures, fresh, dry, intact used as is, chopped, milled, powder or extracts thereof. It is yet also in the scope of the invention wherein materials of GROUP D are one or more herbs selected from a group consisting of at least three herbs: the first herb is a member of the Urticaceae family, preferably first herb is at least one of Urtica diocia (nettle) or Pipturus albidus (mamaki); the second herb is a member of the Nepetoideae family, preferably second herb is at least one of Thymus vulgaris (thyme) or Salvia officinalis (sage); and the third herb is a member of the Lamiaceae family, preferably third herb is at least one of Rosmarinus officinalis (rosemary) or Mentha viridis (spearmint).

EXAMPLE 1

[0136] In one example, at least three of different herbs selected from Group D were mixed. The mixture included 46% of at least one first herb, 27% of at least one second herb and 27% of at least one third herb. Said mixture then boiled in 200 ml deionized water. The obtained extract mixture was analyzed: at least one compound selected from Group A having a GC retention time 11.55 min.; at least one compound selected from Group B having a GC retention time of 11.94 min.; and at least one compound selected from Group C having a GC retention time of 12.69 min.; wherein GC peak area of Group A to Group B ratio is ranging from about 1:7 to about 1:2; peak area of Group B to Group C ratio is ranging from about 1:4 to about 3:2. Peaks area of Group A to Group C ratio was ranging from about 1:12 to 1:3. The filtered extract, allowed to cool to room temperature, was introduced on scalps of various groups of female's and male's for about one hour. One group utilized aforesaid mixture in a liquid solution; Second group utilized aforesaid mixture in a gel form; third group utilized aforesaid mixture in a capsulated manner; forth group utilized aforesaid mixture in cream-like ointment; fifth group utilized aforesaid mixture in a water in oil emulsion; sixth group utilized aforesaid mixture as a foam; seventh group utilized aforesaid mixture in oil-miscible solution; eighth group utilized aforesaid mixture as a milled powder; ninth group utilized aforesaid mixture as (i) soap, (ii) shampoo, and (iii) conditioner. Hair condition (including alopecia's scoring) in those nine test groups was significantly better then controlled group that utilized two-herb based mixtures.

EXAMPLE 2

[0137] Preparation of an Infusion

[0138] No-rinse treatment The herbal mixture (about 10 g) was boiled in 350 ml water, with or without a filter. After about 90 minutes, liquid cools down and reaches room temperature. The color of the water turns dark brown. The cooled mixture was poured on wet hair (in the bath) after the overlap, both on the scalp and on the hair itself including hair ends. Scalp was massaged slightly and was not washed. The mixture is recommended to use after each hair shampooing, at least 3 times a week. The hair can be dried with a hairdryer.

[0139] Preparation of a Cooked Product

[0140] A mixture of the three different herbs selected from Group D (about 10 g, total weight) was used. GC peaks area of Group A to Group B to Group C ratio was about 5:17:45. The said mixture then boiled in either a tea-bag, filtered or in a non-filtered state, in 350 ml of water. When the water reaches a boiling temperature, lower the temperature and further heat (about 60 C.) another about 10 minutes. Wait for it to cool down. Filtration is possible (if filter was not used during cooking).

[0141] Preparation of a Water-Washable Product

[0142] The three different herbs selected from Group D were provided in a mixture (about 10 g) and boiled in 350 ml water. The obtained extract was analyzed by means of GCMS and revealed 4 peaks, at RT=11.55, RT=11.94, RT=12.69 and RT=12.84. The peak areas measured were as follows: RT=11.55, area=5.25, height=25 mm; RT=11.94, area=17.18, height=41 mm; RT=12.69, area=44.89, height=90 mm; and RT=12.84, area=32.69, height=78 mm. The extract was then incorporated in both solvent-based and waterborne cosmetic preparations comprising, inter alia, one or more members of a group consisting of a water-miscible alcohol, such as ethanol, anti-bacterial agents, moisturizers and hydration agents, penetration agents, preservatives, viscosity-controlling agents anesthetics, vitamins, anti-itch actives, botanical extracts, color agents, enzymes, conditioning agents, darkening or lightening agents, nutrient, micronutrient, fragrance, glitter, hair pigment additives, humectants, mica, minerals, oils, polyphenols, silicones or derivatives thereof, sun-blocks, surfactants, vitamins, waxes, solvents, rheology modifiers, suspending agent, thickeners, hair health actives, anti-dandruff actives, anti-oxidants, pigments, abrasives, absorbents, biological actives, buffering agents, chelating agents, opacifying agents, pH adjusters, chelates, perfumes, brighteners, sensates, attractants, dyes, bleaches and any combination thereof.

[0143] Two Methods for Preparation of a Mixture Useful for Treating Scalp and Hair

[0144] First method comprising two steps: First step is admixing an effective measure of at least one first herb selected from Group D, an effective measure of at least one second herb selected from Group D, and an effective measure of at least one third herb selected from Group D; said mixture, when boiled, is characterized by GC retention time where at least one compound selected from Group A having a GC retention time 11.55 min.; at least one compound selected from Group B having a GC retention time of 11.94 min.; and at least one compound selected from Group C having a GC retention time of 12.69 min.; wherein GC peak area of Group A to Group B ratio is ranging from about 1:7 to about 1:2; peak area of Group B to Group C ratio is ranging from about 1:4 to about 3:2; and peak area of Group A to Group C ratio is ranging from about 1:12 to 1:3; and then:

[0145] Second step, immersing said mixture in boiling water for about 10 to about 180 minutes.

[0146] This method was provided useful, inter alia, for treating hair disorders, alopecia, accidental injury, damage to hair follicles, surgical trauma, burn wound, radiation or chemotherapy treatment site, incisional wound, donor site wound from skin transplant and ulcer.

[0147] Additionally or alternatively, the second method comprising three steps. Frist step of admixing an effective measure of at least one first herb selected from Group D, an effective measure of at least one second herb selected from Group D, and an effective measure of at least one third herb selected from Group D; said mixture, when boiled and cooked, is characterized by GC retention time where at least one compound selected from Group A having a GC retention time 11.55 min.; at least one compound selected from Group B having a GC retention time of 11.94 min.; and at least one compound selected from Group C having a GC retention time of 12.69 min.; wherein GC peak area of Group A to Group B ratio is ranging from about 1:7 to about 1:2; peak area of Group B to Group C ratio is ranging from about 1:4 to about 3:2; and peak area of Group A to Group C ratio is ranging from about 1:12 to 1:3.

[0148] Second step of immersing said mixture of in boiling water for about 10 to about 180 minutes; and boiling said mixture; and then

[0149] Third step of cooking the boiled mixture for additional up to 60 minutes in about 40 C. to about 80 C.

[0150] This method was provided useful for, inter alia, providing the treated hair disorders including improving brilliance, fullness, gloss, glow, length, luster, patina, sheen, shine, thickness, and volume.

EXAMPLE 3

[0151] Questionnaires and Results

[0152] Two cosmetic products were tested. The first cosmetic product comprises an effective measure of at least one first herb selected from Group D, an effective measure of at least one second herb selected from Group D, and an effective measure of at least one third herb selected from Group D; said mixture, when boiled, is characterized by GC retention time where at least one compound selected from Group A having a GC retention time 11.55 min.; at least one compound selected from Group B having a GC retention time of 11.94 min.; and at least one compound selected from Group C having a GC retention time of 12.69 min.; wherein GC peak area of Group A to Group B ratio is ranging from about 1:7 to about 1:2; peak area of Group B to Group C ratio is ranging from about 1:4 to about 3:2; and peak area of Group A to Group C ratio is ranging from about 1:12 to 1:3; and then immersing said mixture of (b) in boiling water for about 10 to about 180 minutes. The second cosmetic product comprises an effective measure of at least one compound selected from Group A having a GC retention time 11.55 min.; at least one compound selected from Group B having a GC retention time of 11.94 min.; and at least one compound selected from Group C having a GC retention time of 12.69 min.; wherein GC peak area of Group A to Group B ratio is ranging from about 1:7 to about 1:2; peak area of Group B to Group C ratio is ranging from about 1:4 to about 3:2; and peak area of Group A to Group C ratio is ranging from about 1:12 to 1:3; and then immersing said mixture of (b) in boiling water for about 10 to about 180 minutes.

[0153] A study was performed on 40 women who used the mixture defined above for about two weeks, overall about 18 times the mixture was used for each woman. The results of the questionnaires were very similar. They all answered the questionnaire after 10 times the product was used. The effects were already apparent after a small number of uses. All of them were satisfied with the mixture of the current invention. All the treated human subjects indicated a change in their hair condition, whether they had a specific problem or not. The mixture of the current invention treats a diseased hair, but also with healthy hair but thin, etc.

[0154] According to the detailed questionnaire of 10.sup.th time treatment and the longer examination (18.sup.th time treatment) of one female, the significant improvement in hair loss and overall hair appearance are evident after 5 washings (shampooing), but significant results can be seen after longer use. Once the treatment stops, the hair slowly returns to normal in terms of its symptoms and appearance.

[0155] The questionnaires included the aforementioned queries: Are the instructions for use clear enough? Is the use with the product simple or complicated? Can you characterize the hair smell following use? Did you feel any change in your hair following use with the product? How many treatments are needed for causing the change? What is the main contribution of the product, if any in the following characteristics: Oily hair, hair loss, thin hair, hair volume, feeling fuller hair? Would you like to use the product again? If the product is marketedwhat will be your weekly use? Would you like to use a daily dosage or a weekly dosage? Would you recommend other to use this product? How would you define the product? Any other comments/remarks? The results of the questionnaires are summarized in Table 1 and Table 2 below:

TABLE-US-00001 TABLE 1 The effect of the 10.sup.th time use mixture, according to the above questionnaires. The questionnaires were summarized and evaluated using various characteristics: Shining a shiner hair, a sense of sparkling hair, glamor. Volume a feeling that the hair is more swollen in the positive sense of the word. Instead of flat hair and close to the scalp and face, hair has more presence in terms of volume as a result of repeated use of the product. Feeling fuller hair there is a feeling that the hair is full in terms of the amount of hair on the head. Feeling that there is more hair on your head. A feeling of thickening of the hair there is a feeling that the hair itself is thicker, a feeling that the washing of the plants wraps the hair follicle and creates a kind of cover. Hair is stronger hair feels stronger, less broken, edges less split. The hair fiber is durable and stronger. Healthy hair there is a feeling that the hair is no longer broken easily, does not tear easily. The root is more durable and stronger. The hair itself is more durable. The new hair that grows also feels stronger and healthier. Scalp and hair are less oily scalp is much less oily than it was. A feeling of a clean scalp, both of the scales and of the fat the skin produces. You can wash less often a week and feel that the hair still looks good and still clean, without an oily appearance. Women who used to shampoo every day - while using the product dropped and 3-4 overlaps per week! Every other day. This is significant and facilitates the routine of life and cultivation much more. Reducing hair loss less hair is falling out. Hairs fall much less during the day when the hand on the hair and without any contact with the hair. We examined women with dramatic dropout problems and women with light hair loss. All reported significant improvement. Smell the product has a pleasant smell that lingers over time, a natural smell. The product nourishes any hair type (curly, smooth, corrugated, etc.)

TABLE-US-00002 TABLE 2 The effect of the 18.sup.th time use mixture, according to the above questionnaires: The effect of 18.sup.th time treatment was as follows: The hair is brighter, the hair feels more alive, more pleasant and soft, the scales are gone, the scalp is no longer as greasy as it was, the hair is not oily, the hair feels stronger and fuller. I have thin, volume-less hair, the use of the brew makes my hair become much more volume. The more product users see these changes. Initial change can already be seen between first and third use. A significant change is already seen in the fifth use. In terms of hair loss, after 18 rinses with the product, when I move my hand in my hair I see there are far fewer hairs falling out. The smell of the product is very pleasant.

[0156] Reference is now made to FIG. 1 that depicts a female (Female #1), treated with the mixture of the current invention. Photos (A) were taken 5 hours before shampooing compared to photos taken 5 hours following 18 times of shampooing (B), of the same female individual. Photos were taken in a back view (A1, B1), a side view (A2, B2), and from an overhead view (A3, B3);

[0157] Reference is now made to FIG. 2a that depicts micrographs of hair of Female #1, taken under a light microscope at a 25-fold magnification. Hairs A and B were taken from an individual before use of the mixture, while hairs C and D were taken following treatment of said mixture for about a month. The micrographs were taken by Kaps Som 62 dental microscope with Sony Next camera connected by TV tube.

[0158] Reference is now made to FIG. 2b that depicts Micrographs of hair of Female #1, taken under a light microscope at a 100-fold magnification. Hairs A and B were taken from an individual before use of the mixture, while hairs C and D were taken following treatment of said mixture for 18 times of shampooing, about a month.

[0159] The micrographs were taken by light microscope Olympus B.H-2, connected to an Olympus camera, using DP70 software.

[0160] Reference is now made to FIG. 2c that depicts micrographs of hair of Female #1, taken under a light microscope at a 200-fold magnification. Hairs A and B were taken from an individual before use of the mixture, while hairs C and D were taken following treatment of said mixture 18 times of shampooing, about a month.

[0161] The micrographs were taken by light microscope Olympus B.H-2, connected to an Olympus camera, using DP70 software.

[0162] Reference is now made to FIG. 2d that depicts micrographs of hair of Female #1, taken under a light microscope at a 400-fold magnification. Hairs A and B were taken from an individual before use of the mixture, while hairs C and D were taken following treatment of said mixture 18 times of shampooing, about a month.

[0163] The micrographs were taken by light microscope Olympus B.H-2, connected to an Olympus camera, using DP70 software.

[0164] Reference is now made to FIG. 3 that depicts Micrographs of hair of Female #2, taken under a light microscope at a 100-fold (A), a 200-fold (B), a 400-fold (C) magnification. Hairs A and B and C were taken from an individual following treatment of said mixture for about seven months.

[0165] The micrographs were taken by light microscope Olympus B.H-2, connected to an Olympus camera, using DP70 software.

[0166] Reference is now made to FIG. 4 depicting A GC-MS chromatogram of the mixture according to one embodiment of the invention

EXAMPLE 4

[0167] GC parameters The infusion mixture of the current invention has been analyzed by Gas Chromatograph, GCMS, Model GCD 1800. Preparation for analysis was performed by Basic extraction (pH=10) in dichloromate, 3 times, ratio of 1 to 5 sample to solvent Acid extraction (pH=3) in dichloromethane, 3 times, ratio of 1 to 5 sample to solvent. Consolidation of the extracts and evaporating in vacuum to a volume of 10 ml. Injection of 2 microliters of the sample into the GCMS, using the following program: 60 C for 7 minutes, increasing at 15 C per minute to 320 C, staying at 320 C for 15 minutes.

[0168] GC parameters The infusion mixture of the current invention has been analyzed by Gas Chromatograph, GCMS, Model GCD 1800. Preparation for analysis was performed by Basic extraction (pH=10) in dichloromate, 3 times, ratio of 1 to 5 sample to solvent Acid extraction (pH=3) in dichloromethane, 3 times, ratio of 1 to 5 sample to solvent. Consolidation of the extracts and evaporating in vacuum to a volume of 10 ml. Injection of 2 microliters of the sample into the GCMS, using the following program: 60 C for 7 minutes, increasing at 15 C per minute to 320 C, staying at 320 C for 15 minutes.

[0169] Reference is now made to FIG. 4-7 depicting A GC-MS chromatogram of the mixture according to one embodiment of the invention

[0170] The GCMS Chromatogram, for the analyzed compounds of the first herb revealed 6 peaks, at RT=8.23, RT=8.36, RT=8.43, RT=8.50, RT=8.55 and RT=8.82. The peak areas measured were as follows: RT=8.43, area=6.39; RT=8.36, area=32.40; RT=8.43, area=22.85; RT=8.50, area=15.49; RT=8.55, area=6.63, and RT=8.82, area=16.27. GC chromatogram is depicted in FIGS. 5A and 5B.

[0171] The GCMS Chromatogram, for the analyzed compounds of the second herb revealed 4 peaks, at RT=12.67, RT=12.84, RT=13.14, and RT=18.14. The peak areas measured were as follows: RT=12.67, area=52.22; RT=12.84, area=27.21; RT=13.14, area=7.90; RT=18.14, and area=12.67. GC chromatogram is depicted in FIG. 6.

[0172] The GCMS Chromatogram, for the analyzed compounds of the third herb revealed 5 peaks, at RT=11.52, RT=11.89, RT=15.23, RT=17.21 and RT=18.15. The peak areas measured were as follows: RT=11.52, area=8.73; RT=11.89, area=37.95; RT=15.23, area=7.04; RT=17.21, area=12.32; RT=18.15, and area=33.96. GC chromatogram is depicted in FIG. 7.

[0173] The GCMS Chromatogram, analyzed following preparation of the infusion of the current invention (the mixture), revealed 4 peaks, at RT=11.55, RT=11.94, RT=12.69 and RT=12.84. The peak areas measured were as follows: RT=11.55, area=5.25, height=25 mm; RT=11.94, area=17.18, height =41 mm; RT=12.69, area=44.89, height=90 mm; and RT=12.84, area=32.69, height=78 mm. GC chromatogram is depicted in FIG. 4.

[0174] Having thus described a preferred embodiment, it should be apparent to those skilled in the art that certain advantages of the described method and apparatus have been achieved. It should also be appreciated that various modifications, adaptations, and alternative embodiments thereof may be made within the scope and spirit of the present invention. The invention is further defined by the following claims.

EXAMPLE 5

[0175] Chirality: Chirality test, for measuring the specific rotation values of the compounds and the mixture of the invention was performed using Autopol automatic polarimeter by Rudolph research analytical.

[0176] The measurements of the three compounds and their mixture reveled the following values: [0177] a. The first herb has a premixing specific rotation value of [].sub.D.sup.20=99; [0178] b. The second herb has a premixing specific rotation value of [].sub.D.sup.20=+33; [0179] c. The third herb has a premixing specific rotation value of [].sub.D.sup.20=5; and [0180] d. The mixture of herbs has a post mixture specific rotation value of [].sub.D.sup.20=+18.

[0181] In a preferred embodiment first herb is Urtica dioica (nettle) or Pipturus albidus (mamaki), second herb is Thymus vulgaris (thyme) or Salvia officinalis (sage) and third herb is Rosmarinus officinalis (rosemary) or Mentha viridis (spearmint).

EXAMPLE 6

[0182] A kit and use instructions: A preferred embodiment discloses the kit for treatment of scalp and hair is packed and contains: a clear bottle containing an infuser (FIG. 8A), and sealed sachets (FIG. 8B), each of the sachets includes a tea bag made of organza (or nylon). The tea bag includes about 7 grams of the mixture (FIG. 8C); see technical specification of the bag in Table 3.

[0183] Directions for use: boil tap water; fill up the bottle (FIG. 8A) up to 200 ml with boiled water; open one sachet and take out the tea bag; insert the contents of the tea bag into the boiled water in the bottle; mix thoroughly, wait for 10 minutes, add tap water up to 500 ml; check if temperature is suitable for skin treatment; and then pour on scalp and hair (after the hair was shampooed and rinsed); dry with a towel; leave on scalp and hair until next shampooing.

[0184] Recommended frequency for use: at least twice a week, preferably three times a week.

EXAMPLE 7

[0185] The term leachable refers herein after being capable of being removed from a substance by a percolating liquid.

[0186] The term Miscibility/refers herein after to the property of two substances to mix in all proportions (that is, to fully dissolve in each other at any concentration), forming a homogeneous solution.

[0187] A preferred embodiment discloses a synergic water based herbal mixture for the use of treating scalp and hair-disorders, comprising at least three herbs, wherein the first herb is a member of the Urticaceae family, preferably first herb is at least one of Urtica diocia (nettle) or Pipturus albidus (mamaki), having premixing specific rotation value (PSRV) [].sub.D.sup.20=99 and GCMS 6 peaks within RTs 8.23-8.82 min.; the second herb is a member of the Nepetoideae family, preferably second herb is at least one of Thymus vulgaris (thyme) or Salvia officinalis (sage), having a PSRV=+33 and RTs 12.67, 12.84, 13.14 and 18.14 min.; and the third herb is a member of the Lamiaceae family, preferably third herb is at least one of Rosmarinus officinalis (rosemary) or Mentha viridis (spearmint), having a PSRV-5 and RTs 11.52, 11.59, 15.53, 17.21 and 18.15 min; wherein said mixture is characterized by post mixture specific rotation value and by GCMS first, second and third peaks of retention time 11.55, 11.94 and 12.69 min., respectively; wherein said mixture is characterized by said first peak (11.55 min) to said second peak (11.94 min) ratio between 1:7 to 1:2; said second peak to said third peak (12.69 min.) ratio between 1:4 to 3:2; and said first peak to said third peak ratio of between 1:12 to 1:3 as determined by GCMS peak areas; and further wherein said GCMS is Model GCD 1800, utilized under the following conditions: preparation for analysis was performed by basic extraction (pH=10) in dichloromethane, 3 times, ratio of 1 to 5 sample to solvent; acid extraction (pH=3) in dichloromethane, 3 times, ratio of 1 to 5 sample to solvent; consolidation of the extracts and evaporating in vacuum to a volume of 10 ml; injection of 2 microliters of the sample into the GCMS, using the following program: 60 degrees C. for 7 minutes, increasing at 15 degrees C. per minute to 320 degrees C., staying at 320 degrees C. for 15 minutes.

EXAMPLE 8

[0188] Electron Microscopy Analysis

[0189] The experimenter, female #3, is a female, age 24, who has thin, falling and oily hair; and also suffers of hair thinning. The experimenter was treated for a month, at least twice a week, and was photographed in two time points during the treatment period: [0190] a. Before the treatmentthe scalp was photographed at various directions. Hairs where taken from 6 different locations on the scalp (n=3 hairs for each location; see FIGS. 10-15). [0191] b. After using the mixture for about a month, hairs were taken from the same 6 locations on the scalp and were compared by the electronic microscope analysis.

[0192] The results show a significant (P value=5.47E-07, Student's T-test,), thickening of the hair after use of a month (see Table 4)

TABLE-US-00003 TABLE 4 Hair width before and after treatment (as determined by Electron microscopy) LOCATION 6 LOCATION 5 LOCATION 4 LOCATION 3 LOCATION 2 LOCATION 1 BEFORE TREATMENT (hair width m) 86.6 88.0 76.0 67.2 47.1 61.2 90.0 88.0 69.8 66.2 55.7 71.8 85.4 72.3 54.6 51.4 69.3 AVERAGE 71.6 87.3 88.0 72.7 62.7 51.4 67.4 SD 2.6 1.7 0.0 2.2 5.0 3.0 3.9 AFTER TREATMENT (hair width m) 106.3 112.6 91.3 72.3 76.6 108.0 97.3 104.1 88.7 72.6 89.5 105.7 82.7 102.6 73.5 90.7 74.5 AVERAGE 92.0 95.4 106.4 84.5 78.5 80.2 106.9 SD 5.5 8.4 3.8 6.8 7.5 5.7 0.9 t TEST 5.47E07

EXAMPLE 9

[0193] Synergy: In addition to evaluating the effects of the mixtures of the current invention, the effects of each individual herb comprising the mixtures were assessed as well in order to assess synergistic effects. Eight subjects, Female #3-10, aged 24-47, who suffer from thin, flat hair or hair loss were treated for a period of three weeks, either with a rosemary extract (Female #3), thyme extract (Female #4), nettle extract (Female #5), mamaki extract (Female #6), sage extract (Female #7), spearmint extract (Female #8), a mixture comprising thyme, rosemary and nettle (Mix 1, Female #9) or a mixture comprising sage, mamaki and spearmint (Mix 2, Female #10). Subjects washed their hair with the boiled extracts at least twice a week, and hairs from the frontal area of the scalp were sampled and analyzed in two different time points during the treatment period: [0194] a. Before the treatmenthairs from the frontal area of the scalp of each subject were collected and the hairs' width was measured under a microscope. [0195] b. After 3 weeks of using either the rosemary extract, thyme extract, nettle extract, mamaki extract, sage extract, spearmint extract or the mixtures of the current invention ({rosemary+nettle+thyme} or {sage+spearmint+mamaki}), hairs from the frontal area of the scalp of each subject were collected and the hairs' width was measured under a microscope.

[0196] The results show a significant increase in the hair's width only after using the mixtures of the current invention (see Table 5). The average hair's width increased by 62.62% for mixture 1 and by 41% for mixture 2, compared to untreated hair, whereas for thyme, sage, rosemary, mint, nettle and mamaki extracts the average increase was 1.03%, 0.67%, 6.93%, 5.15%, 10.86% and 4.13% respectively. Furthermore, the relative improvements observed after using the rosemary and nettle extracts were significant, but not as statistically significant as the results obtained after using the mixtures. These results confirm that the mixtures of the current invention have synergistic effects compared to using each herb separately, as their efficacy surpasses the total summing of the individual treatments.

TABLE-US-00004 TABLE 5 Hair width before and after treatment with each herb separately and the mixtures of the current invention (as determined by electron microscopy). BEFORE TREATMENT (hair width AFTER TREATMENT (hair width m) m) thyme % sage % rosemary % mint % sample 1 61.11 63.9 61.22 68.14 63.98 sample 2 64.6 65.7 63.55 69.36 72.56 sample 3 73.08 62.9 62.78 70.12 66.34 sample 4 63.59 67.89 71.89 72.34 68.87 sample 5 62.31 70.2 65.89 69.78 64.57 sample 6 65.37 63.5 67.41 67.37 73.85 AVG 65.01 65.68 1.03 65.45 0.67 69.52 6.93 68.36 5.15 SD 4.24 2.86 3.85 1.73 4.139 t-TEST 0.75 0.85 0.04 0.196 AFTER TREATMENT (hair width m) nettle % mamaki % MIX 1 % MIX 2 % sample 1 79.58 62.39 112.65 94.35 sample 2 74.32 64.75 104.18 92.87 sample 3 72.68 61.85 102.64 85.63 sample 4 65.48 74.59 106.47 89.42 sample 5 70.92 72.84 109.82 90.63 sample 6 69.45 69.76 98.56 96.95 AVG 72.07 10.86 67.70 4.13 105.72 62.62 91.64 41 SD 4.76 5.46 5.06 3.98 t-TEST 0.02 0.36 3.3E08 5.5E07 AVG = average, SD = standard deviation, the % represent the average increase in the hair's width. MIX 1 = a mixture comprising rosemary, thyme and nettle. MIX 2 = a mixture comprising sage, spearmint and mamaki.