METHODS FOR THE DETECTION OF AUTOLOGOUS BLOOD-DOPING

Abstract

The present invention relates to the identification of peptides, and the corresponding proteins, that can be used in methods for the detection of autologous blood doping. More specifically, the invention relates to methods comprising tryptic digestion of samples of isolated red blood cell (RBC), specifically isolated RBC cytosol, followed by peptide mapping using liquid chromatography tandem-mass spectroscopy (LC-MS/MS). The methods according to the invention which enable detection of increased levels of certain peptides in samples from subjects that have been subjected to autologous blood doping, compared to samples from non-doped control subjects.

Claims

1. A method for detection of autologous blood-doping with cryopreserved blood in a subject, said method comprising the step: i) identifying whether said subject has or has not been autologous blood doped based on differences in level of one or more specific peptides between an isolated red blood cell cytosol sample prepared from a blood sample from said subject compared to the level of the same specific peptides from an isolated red blood cell cytosol sample prepared from a reference blood sample, such levels having been determined by generation of a proteolytic peptide map for each of the blood sample and the reference blood sample.

2. A method as claimed in claim 1 further comprising performing before step (i) one or more of the steps of: x) determining the level of one or more specific peptide in the isolated red blood cell cytosol sample prepared from the blood sample obtained from said subject; y) determining the level of one or more specific peptide in an isolated red blood cell cytosol sample prepared from a reference blood sample; z) determining any difference in level of said one or more specific peptides compared to the level of the same specific peptides in a reference blood sample.

3. A method as claimed in either of claim 1 or 2, said method comprising the steps: a) generating a proteolytic peptide map of an isolated red blood cell cytosol sample prepared from a blood sample obtained from said subject; b) determining the levels of one or more specific peptide identified in the peptide map obtained in step a) c) determining the difference in level of said one or more specific peptides compared to the level of the same specific peptides in reference peptide maps obtained from an isolated red blood cell cytosol sample prepared from a reference blood sample; and d) identifying whether said subject has or has not been autologous blood doped with cryopreserved blood based on differences in the level of said specific peptides compared to the level of the same specific peptides in said reference peptide maps.

4. A method as claimed in any previous claim wherein the reference blood sample is from a non-doped subject.

5. The method according to any previous claim wherein said one or more specific peptides is or are one or more peptides derived from one or more of the proteins listed in Table 3.

6. The method according to any previous claim wherein said one or more specific peptides is or are one or more peptides selected from the list of peptides comprising SEQ ID Nos: 1-78.

7. The method according to any previous claim wherein said one or more specific peptides is or are 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, or 78 peptides selected from the list of peptides comprising SEQ ID Nos: 1-78.

8. The method according to any previous claim wherein said one or more specific peptides is or are one or more peptides derived from one or more of the proteins selected from: Threonine-tRNA ligase, cytoplasmic; 26S proteasome non-ATPase regulatory subunit 4; Isoform 2 of T-complex protein 1 subunit theta; Tubulin beta-4B chain UV excision repair protein RAD23 homolog B; 26S proteasome non-ATPase regulatory subunit 13; Isoform 2 of Ubiquitin-like modifier-activating enzyme 1; Isoform 3 of Calpastatin; Uroporphyrinogen-III synthase; and Casein kinase II subunit alpha.

9. The method according to any previous claim wherein said one or more specific peptides is or are one or more peptides consisting of the amino acid sequence of one of the peptides SEQ ID Nos: 1, 7, 8, 9, 10, 12, 14, 22, 24, 34, and 78.

10. The method according to any previous claims wherein the step of generating the proteolytic peptide map comprises protease digestion that is performed using one or more of the proteases selected from trypsin, chymotrypsin, Lys-C, Gly-C, Asp-N, Arg-C, papain.

11. The method according to claim 10 wherein the protease digestion is performed by trypsin digestion.

12. The method according to any previous claim wherein the peptide mapping is performed by the combination of liquid chromatography (LC) with mass spectrometry (MS), and preferably wherein the MS is tandem mass spectrometry (MS/MS).

13. Use of a peptide selected from the list of peptides comprising the peptides SEQ ID Nos: 1-78 in a method as described in any one of the preceding claims for detection of autologous blood-doping with cryopreserved blood.

14. A peptide consisting of the amino acid sequence of one the peptides SEQ ID Nos:1, 7, 8, 14, and 34.

15. A kit comprising one or more of the peptides according to claim 14.

16. A method for the identification of biomarkers for the detection of autologous blood-doping with cryopreserved blood, said method comprising the steps; i) identifying differences in the level of one or more peptides between an isolated red blood cell cytosol sample prepared from blood samples obtained from one or more subjects having received an infusion of autologous blood and an isolated red blood cell cytosol sample prepared from blood samples obtained from one or more control subjects not having received an infusion of autologous blood, such levels having been identified following generation a proteolytic peptide map of the blood samples; and ii) identifying peptides being present in significant different levels, as a biomarker for autologous blood doping with cryopreserved blood.

17. A method as claimed in claim 16, said method comprising the steps; i) generating proteolytic peptide maps of an isolated red blood cell cytosol sample prepared from blood samples obtained from one or more individuals having received an infusion of autologous blood, ii) generating proteolytic peptide maps of an isolated red blood cell cytosol sample prepared from blood samples obtained from one or more control individuals not having received an infusion of autologous blood, iii) identifying differences in the level of one or more peptides in the peptide maps obtained in step i) compared to peptide maps obtained in step ii), and iv) identifying peptides being present in significant different levels, and the corresponding proteins, as a biomarker for autologous blood doping with cryopreserved blood.

18. The method according to claim 16 or 17, wherein said cytosol is depleted of hemoglobin or has a reduced level of hemoglobin.

Description

DESCRIPTION OF THE INVENTION

[0012] The present inventors have now developed a method comprising tryptic digestion of samples of isolated red blood cells (RBC), specifically isolated RBC cytosol samples, followed by peptide mapping using liquid chromatography tandem-mass spectroscopy (LC-MS/MS). The methods according to the invention enable detection of increased levels of certain peptides in samples from subjects that have been subjected to autologous blood doping, compared to samples from non-doped control subjects.

[0013] These peptides, and the related proteins, have, in themselves, utility as biomarkers for the detection of autologous blood-doping.

[0014] As discussed above, autologous blood doping requires storage of donated blood for a period of time that requires the use of cryopreservation and freeze storage. The present invention is therefore aimed at detecting changes that occur in the red blood cell proteins during the freeze-thawing cycle and using them to identify samples that have undergone that process, including identifying blood samples from subjects that have undergone autologous blood doping.

[0015] The inventors have identified that levels of individual tryptic peptides generated during the proteolytic digestion are different between doped and non-doped blood (as seen, for example, in LC/MS-MS measurements after digestion). Without wishing to be bound by theory, the inventors believe that the cryopreservation, freeze storage and/or thawing process may cause structural changes in certain proteins in the red blood cells, for example red blood cell cytosolic proteins. These structural changes appear to remain even after re-infusion and so appear to alter the accessibility of certain cleavage sites for proteolytic enzymes, such as accessibility to certain trypsin sites. This in turn can create the changes in levels of individual tryptic peptides generated during the proteolytic digestion. Therefore, the changes in the levels of certain peptides in the tryptic map is indicative of changes in the structure of the related proteins.

[0016] Accordingly, the present invention provides methods for the detection of autologous blood-doping in a subject, said method comprising detection of differences in the levels (also referred to as the amounts) of peptides obtained from blood samples, including red blood cells, following generation of a proteolytic peptide map of red blood cell proteins, preferably red blood cell cytosolic proteins.

[0017] In one aspect of the invention there is provided a method for detection of autologous blood-doping in a subject, said method comprising the step: [0018] i) identifying whether said subject has or has not been autologous blood doped based on differences in level of one or more specific peptides between a blood sample from said subject compared to the level of the same specific peptides from a reference blood sample, such levels having been determined by generation of a proteolytic peptide map for each of the blood sample and the reference blood sample.

[0019] Peptide mapping of biological samples has been described in numerous publications including, for example in [Saraswathy et al. Protein Identification by Peptide Mass Fingerprinting. In Concepts and Techniques in Genomics and Proteomics, 2011. Woodhead Publishing Ltd.; and in Cottrell, Protein identification using MS/MS data. J Proteomics 2011. 74(10), 1842-1851.]

[0020] In one option the method may further comprise performing, before step (i), one or more of the steps of [0021] x) determining the level of one or more specific peptide in the blood sample obtained from said subject; [0022] y) determining the level of one or more specific peptide in a reference blood sample; [0023] z) determining any difference in level of said one or more specific peptides compared to the level of the same specific peptides in a reference blood sample.

[0024] The method may comprise the following steps: [0025] a) generating a proteolytic peptide map of a blood sample obtained from said subject; [0026] b) determining the levels of one or more specific peptide identified in the peptide map obtained in step a); [0027] c) determining the difference in level of said one or more specific peptides compared to the level of the same specific peptides in reference peptide maps obtained from a reference blood sample; and [0028] d) identifying whether said subject has or has not been autologous blood doped based on differences in the level of said specific peptides compared to the level of the same specific peptides in said reference peptide maps.

[0029] Preferably, the reference blood sample is from a non-doped subject. Preferably, reference blood samples are collected from multiple non-doped subjects of different age, gender and ethnicity, both athletes and non-athletes.

[0030] In another aspect of the invention provides methods for detection of autologous blood-doping in a subject, said method comprising the steps: [0031] a) generating a proteolytic peptide map of a blood sample obtained from said subject; [0032] b) determining the level of one or more specific peptides identified in the peptide map obtained in step a); [0033] c) determining the difference in level of said one or more specific peptides compared to the level of the same specific peptides in reference peptide maps obtained from a reference population of non-doped subjects; and [0034] c) identifying said subject as being autologous blood doped based on differences in the level of said specific peptides compared to the level of the same peptides in said reference peptide maps.

[0035] In a further aspect of the invention there is provided a method for determining the difference in level of one or more specific peptides in a blood sample from a subject, the method comprising: [0036] a) generating a proteolytic peptide map of the blood sample obtained from said subject; [0037] b) determining or measuring the levels of one or more specific peptide identified in the peptide map obtained in step a); and, [0038] c) determining and/or reporting the difference in level of said one or more specific peptides compared to the level of the same specific peptides in reference peptide maps obtained from a reference population of subjects.

[0039] In a yet further aspect of the invention, there is provided a method for determining and/or reporting the difference in level of one or more specific peptides in a blood sample from a subject, the method comprising: comparing the levels of one or more specific peptides in a peptide map generated by proteolytic digestion of the blood sample obtained from said subject, to the level of the same specific peptides in reference peptide maps obtained from a reference population of subjects, thereby determining and/or reporting the difference in level of said one or more specific peptides.

[0040] In an alternative aspect of the invention there is provided a method for detecting autologous blood-doping in a subject, said method comprising: [0041] a) generating a proteolytic peptide map of red blood cells (RBCs) or cytosol thereof, wherein the RBCs are isolated from a blood sample obtained from said subject; [0042] b) determining the levels of one or more specific peptide identified in the peptide map obtained in step a); [0043] c) determining the difference in level of said one or more specific peptides compared to the level of the same specific peptides in reference peptide maps obtained from a reference population of non-doped subjects; and, [0044] d) reporting said subject as being autologous blood doped based on differences in the level of said specific peptides compared to the level of the same specific peptides in said reference peptide maps.

[0045] In a yet further aspect of the invention there is provided a method for detecting autologous blood-doping in a subject, said method comprising: [0046] a) isolating red blood cells (RBCs) or cytosol thereof, from a blood sample obtained from said subject; [0047] b) generating a proteolytic peptide map of said RBCs or cytosol thereof; [0048] c) determining and/or measuring the levels of one or more specific peptide identified in the peptide map obtained in step a), for ascertaining the difference in level of said one or more specific peptides compared to the level of the same specific peptides in reference peptide maps obtained from a reference population of subjects; and, [0049] d) reporting said subject as being autologous blood doped based on differences in the level of said specific peptides compared to the level of the same specific peptides in said reference peptide maps.

[0050] In the aspects of the invention described above, one or more of the following embodiments may apply.

[0051] In one embodiment, the reference blood sample is from a non-doped subject. Preferably, reference blood samples are collected from multiple non-doped subjects of different age, gender and ethnicity, both athletes and non-athletes.

[0052] In one embodiment, the difference in level of each said specific peptide compared to the level of the same peptide in said reference peptide maps can be more than 10%, such as more than 20%, 30%, 40%, 50%, such as preferably more than 60%, 70%, 80%, 90%, even more preferably more than 100%, 150%, or even more preferably than 200%. The amount of an increase can be can be more than 10%, such as more than 20%, 30%, 40%, 50%, such as preferably more than 60%, 70%, 80%, 90%, even more preferably more than 100%, 150%, or even more preferably than 200%. The amount of a decrease can be more than 10%, such as more than 20%, 30%, 40%, 50%, such as preferably more than 60%, 70%, 80%, 90%, up to an including a 100% decrease i.e. a complete absence of the peptide.

[0053] In one embodiment said specific peptides can be one or more peptides derived from one or more of the proteins listed in Table 3.

[0054] In one embodiment said specific peptides can be one or more peptides selected from the list of peptides comprising the peptides SEQ ID Nos: 1-78.

[0055] Said specific peptides can be 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, or 78 peptides selected from the list of peptides comprising SEQ ID Nos: 1-78.

[0056] In one preferred embodiment said specific peptides can be one or more peptides selected from the list of peptides comprising SEQ ID Nos: 1, 2, 7, 8, 9, 10, 12, 14, 15, 19, 22, 23, 24, 31, 34, 51, 77, 78.

[0057] Preferably, said specific peptides can be 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, or 18 peptides from the list of peptides SEQ ID Nos: 1, 2, 7, 8, 9, 10, 12, 14, 15, 19, 22, 23, 24, 31, 34, 51, 77, 78.

[0058] In one preferred embodiment said specific peptides can be one or more peptides selected from the list of peptides comprising SEQ ID Nos: 1, 7, 8, 9, 12, 14, 22, 24, 78.

[0059] Preferably, said specific peptides can be 1, 2, 3, 4, 5, 6, 7, 8, or 9 peptides from the list of peptides SEQ ID Nos: 1, 7, 8, 9, 12, 14, 22, 24, 78.

[0060] Preferably, said specific peptides can be 5 to 10 peptides selected from the list of peptides comprising SEQ ID Nos: 1-78, or 5 to 10 peptides selected from the list of peptides comprising SEQ ID Nos: 1, 2, 7, 8, 9, 10, 12, 14, 15, 19, 22, 23, 24, 31, 34, 51, 77, 78, or 5 to 9 peptides selected from the list of peptides comprising SEQ ID Nos: 1, 7, 8, 9, 12, 14, 22, 24, 78.

[0061] Preferably the blood sample to be analyzed in the methods according to the invention is a red blood cell sample obtained from the subject to be tested. Even more preferably the blood sample to be analyzed is an isolated red blood cell cytosol sample obtained from the subject to be tested.

[0062] Accordingly, in one embodiment the methods according to the invention can comprise the step of isolating red blood cells from a blood sample obtained from the subject to be tested, prior to the step comprising protease digestion of the obtained red blood cells. Isolation of red blood cells can be performed using any laboratory technique for such isolation, including centrifugation.

[0063] Accordingly, in another embodiment the methods according to the invention can comprise the step of isolating red blood cells from a blood sample obtained from the subject to be tested, and a further step comprising isolation of the red blood cell cytosolic fraction, prior to the step comprising protease digestion of the obtained red blood cytosol. The red blood cell cytosolic fraction can be prepared by hypotonic lysis of red blood cells and subsequent removal of the red blood cell membranes by centrifugation.

[0064] Accordingly, in another embodiment the methods according to the invention can comprise the step of isolating red blood cells from a blood sample obtained from the subject to be tested, and a further step comprising isolation of the red blood cell cytosolic fraction, and yet another step comprising depletion of the cytosolic fraction of hemoglobin, prior to the step comprising protease digestion of the obtained red blood cytosol.

[0065] The blood sample, the isolated red blood sample, or the isolated red blood cell cytosol sample to be tested can further be supplemented with known amounts of one or more reference protein or reference peptide. One or more reference peptides can contain one or more amino acids containing a stable heavy isotope label providing the labelled peptide with a defined increase in molecular weight. The isotope label can be .sup.13C, or .sup.15N.

[0066] The reference peptides can be one or more of the peptides selected from the list of peptides comprising SEQ ID Nos: 1-78.

[0067] Preferably the proteolytic peptide map is a tryptic peptide map, i.e. the proteolytic digestion of the sample to be tested is performed by trypsin digestion. The proteolytic digestion can also be performed by using one or more of the proteases selected from trypsin, chymotrypsin, Lys-C, Gly-C, Asp-N, Arg-C, papain. [Saraswathy et al., 2011. (supra)]

[0068] The peptide mapping can be performed by the combination of liquid chromatography (LC) with mass spectrometry (MS), preferably the MS is tandem mass spectrometry (MS/MS). The liquid chromatography can be high-performance liquid chromatography (HPLC), also known as high pressure liquid chromatography, ultra performance (pressure) liquid chromatography (UPLC), or ultra-high performance (pressure) liquid chromatography (UHPLC). [Fekete et al. Current and future trends in UHPLC. Trends Anal Chem 2014. 63, 2-13].

[0069] One aspect of the invention provides an isolated peptide selected from the list of peptides comprising the peptides SEQ ID Nos. 1-35, and 37-78.

[0070] Preferably the isolated peptide is selected from the list of peptides comprising the peptides SEQ ID Nos: 1, 2, 7, 8, 9, 10, 12, 14, 15, 19, 22, 23, 24, 31, 34, 51, 77, and 78.

[0071] One aspect of the invention provides a kit comprising 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 70, 71, 71, 73, 74, 75, 76, 77 or 78 peptides from the list of peptides SEQ ID Nos: 1-78.

[0072] One aspect of the invention provides a kit comprising 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 70, 71, 71, 73, 74, 75, 76, 77, or 78 of the peptides from the list of peptides SEQ ID Nos: 1-78 for use in a method according to the invention.

[0073] Preferably the kits of the invention have one or more of the following optional features: [0074] (1) the peptides are stored in individual containers; [0075] (2) combinations of peptides are stored in individual containers; [0076] (3) the peptides are stored in solution; [0077] (4) the peptides are lyophilized; [0078] (5) the peptides are provided in an amount of 450 fmol up to 60000 fmol; [0079] (6) the peptides are, when stored in solution, provided in a concentration of 0.025 M to 10000 M.

[0080] The kit may further comprise peptides of non-human origin for quality and performance assurance, e.g. peptides with proteolytic sites for performance control of the proteolytic step.

[0081] Another aspect of the invention provides a method for the identification of biomarkers for the detection of autologous blood-doping, said method comprising the steps; [0082] i) identifying differences in the level of one or more peptides between blood samples obtained from one or more subjects having received an infusion of autologous blood and from blood samples obtained from one or more control subjects not having received an infusion of autologous blood, such levels having been identified following generation a proteolytic peptide map of the blood samples; and [0083] ii) identifying peptides being present in significant different levels, as a biomarker for autologous blood doping.

[0084] Preferably, the proteolytic peptide map is generated from red blood cells obtained from the blood sample(s).

[0085] Such a method may comprise the steps; [0086] i) generating proteolytic peptide maps of red blood cells prepared from blood samples obtained from one or more individuals having received an infusion of autologous blood, [0087] ii) generating proteolytic peptide maps of red blood cells prepared from blood samples obtained from one or more control individuals not having received an infusion of autologous blood, [0088] iii) identifying differences in the level of one or more peptides in the peptide maps obtained in step i) compared to peptide maps obtained in step ii), and [0089] iv) identifying peptides being present in significant different levels, and the corresponding proteins, as biomarker for autologous blood doping.

[0090] In an alternative aspect of the invention, there is provided a method for identifying proteins having modified expression level post autologous blood-doping, said method comprising: comparing the level of one or more peptides in peptide maps generated by proteolytic digestion of red blood cells (RBCs) or cytosol thereof prepared from blood samples obtained from one or more individuals having received an infusion of autologous blood, to the level of the same specific peptides in reference peptide maps generated by proteolytic digestion of red blood cells (RBCs) or cytosol thereof prepared from blood samples obtained from one or more individuals not having received an infusion of autologous blood, thereby identifying peptides having modified expression level post autologous blood-doping.

[0091] Such method may alternatively comprise the steps: [0092] i) generating proteolytic peptide maps of red blood cells (RBCs) or cytosol thereof prepared from blood samples obtained from one or more individuals having received an infusion of autologous blood, [0093] ii) generating proteolytic peptide maps of RBCs or cytosol thereof prepared from blood samples obtained from one or more control individuals not having received an infusion of autologous blood, [0094] iii) identifying differences in the level of one or more peptides in the peptide maps obtained in step i) compared to peptide maps obtained in step ii), and [0095] iv) identifying peptides being present in significant different levels, thereby identifying the corresponding proteins as having modified expression level post autologous blood-doping.

[0096] The above two aspects of the invention may include one or more of the following embodiments.

[0097] Preferably the red blood sample to be analyzed is an isolated red blood cell cytosol sample.

[0098] Preferably the cytosol sample has been depleted of hemoglobin or has a reduced level of hemoglobin.

[0099] Preferably the proteolytic peptide map is a tryptic peptide map, i.e. the proteolytic digestion of the proteins is performed by trypsin digestion. The proteolytic digestion can also be performed by using one or more of the proteases selected from trypsin, chymotrypsin, Lys-C, Gly-C, Asp-N, Arg-C, papain.

[0100] The peptide mapping can be performed by the combination of liquid chromatography (LC) with mass spectrometry (MS), preferably the MS is tandem mass spectrometry (MS/MS). The liquid chromatography can be high-performance liquid chromatography (HPLC), also known as high pressure liquid chromatography, ultra performance (pressure) liquid chromatography (UPLC), or ultra-high performance (pressure) liquid chromatography (UHPLC).

[0101] The kits and methods of the invention as described in the various aspects above may include or can allow for the identification of peptides of interest, whereby samples of known peptides allow the testing lab to positively identify the corresponding peptides in the sample from the subject and the control sample.

[0102] Therefore, the methods may comprise the additional step of identifying peptides of interest by comparison to reference peptides.

[0103] In one embodiment, such identification can be performed by the combination of liquid chromatography (LC) with mass spectrometry (MS), preferably the MS is tandem mass spectrometry (MS/MS). The liquid chromatography can be high-performance liquid chromatography (HPLC), also known as high pressure liquid chromatography, ultra performance (pressure) liquid chromatography (UPLC), or ultra-high performance (pressure) liquid chromatography (UHPLC).

DEFINITIONS

[0104] By subject we mean an individual animal. The animal may be a mammal, including a human, a horse or a dog. The subject is typically, but not exclusively, involved in competitive and/or professional sports.

[0105] By blood donation we mean the collection of blood from the subject for storage and later re-infusion into the subject. We do not mean the taking of a small blood sample for testing purposes. Typically, donation applies to volumes of 50 mL-500 mL. A blood sample would more typically be a volume of approximately 50 L-5 mL.

[0106] By autologous blood transfusion or re-infusion we mean the re-introduction of stored blood previously obtained from the subject back into the same subject.

[0107] By blood sample we include samples of blood including but not limited to whole blood samples, and isolated blood cells.

[0108] By difference in level we mean either an increased or decreased amount compared to the other sample, for example an increase in the amount of a particular peptide in the test sample compared to the reference sample; or alternatively a decrease in the amount of a particular peptide in the test sample compared to the reference sample. The amount of an increase can be can be more than 10%, such as more than 20%, 30%, 40%, 50%, such as preferably more than 60%, 70%, 80%, 90%, even more preferably more than 100%, 150%, or even more preferably than 200%. The amount of a decrease can be more than 10%, such as more than 20%, 30%, 40%, 50%, such as preferably more than 60%, 70%, 80%, 90%, up to an including a 100% decrease i.e. a complete absence of the peptide.

[0109] By proteolytic peptide map we mean the identification of the various peptides in a sample following proteolytic break down of proteins into such smaller peptides. The map typically takes the form of peptides being defined by mass and/or sequence. [Thiede et al. Peptide mass fingerprinting. Methods 2005. 35, 237-247].

EXAMPLES

Material & Methods

[0110] Subjects

[0111] In total, 14 individuals participated in the study. Seven healthy women and seven healthy men were recruited for donation, one unit for women and two units for men, where one unit corresponds to 450 mL blood.

[0112] Study Design

TABLE-US-00001 TABLE 1 Time line N = 7 Men; Week 7 Women 0 1 2 3 4 5 6 7 8 9 VO.sub.2MAX T Blood sampling .box-tangle-solidup. .box-tangle-solidup. .box-tangle-solidup. .box-tangle-solidup..circle-solid..circle-solid. .circle-solid. .circle-solid. .circle-solid. .circle-solid. Blood Donation D D RBC re-infusion R State of Clean Donated Doped Transfusion group Time lines showing blood donation and re-infusion, blood sampling and VO.sub.2MAX testing for the discovery phase study. In total, 7 men and 7 women have participated, and 10 samples (.box-tangle-solidup. clean sample, .circle-solid. doped sample) and 3 bag-samples were taken from each person. D; Donation of one unit (450 mL) blood (for women at week 3 only). R; Re-infusion of RBC. T; Testing of physical performance.

[0113] Blood Sampling, Donation and Transfusion

[0114] After an initial treadmill running test of VO.sub.2MAX, one blood sample was taken each week for three weeks to establish individual baseline values. On week 2 and 3, one unit (450 mL) whole blood was donated by all men, and all women donated 450 mL on week 3 only. Blood sampling, donation, cryopreservation of donated blood and re-infusion was all performed at the Centre for Apheresis and stem cell handling, Karolinska University Hospital, Huddinge, Sweden. Blood donation followed standard hospital procedures in Sweden regarding illnesses, staying abroad, tattoos or drugs. Red blood cells were isolated and processed for cryopreservation as described elsewhere [Hutt et al. Transfusion of cryopreserved human red blood cells into healthy humans is associated with rapid extravascular hemolysis without a proinflammatory cytokine response. Transfusion. 2013; 53(1):28-33.) and RBCs stored at 80 C. for 3-4 weeks, unit one and two, respectively, before RBC re-infusion. Re-infusion of both units of washed RBC took place three weeks after donation of the first unit. Blood samples (14.5 mL EDTA) were taken.

[0115] Blood Collection

[0116] 3.5 mL venous blood was drawn from each subject and collected in BD Vacationer Blood Collection Tubes containing EDTA (BD). Tubes were slowly turned 20 times and kept at room temperature for 30 minutes after which they were kept cold until further analysis.

[0117] Preparation of Erythrocytes from Fresh Blood

[0118] Lysis buffer (5 mM phosphate buffer with 1 mM EDTA, pH 7.6) and wash buffer (5 mM phosphate buffer with 1 mM EDTA and 150 mM NaCl, pH 7.6) were prepared one day before use. On day of use protein inhibitor tablets (Artnr. 05056489001, Roche Applied Science) were added to buffers (1 tablet/50 mL lysis buffer, 1 tablet/100 mL wash buffer). 35 mL cold wash buffer was added to centrifuge tubes (Beckman 50 mL). 1 mL blood was transferred to centrifuge tubes with buffer, mixed gently and centrifuged (Beckman Coulter, Avanti J-20 XP, JA17 or 25.50 rotor) at 1300g, 8 C. for 10 min. The plasma and buffy coat fractions were carefully removed and discarded. 1.0 mL of the RBC fraction was transferred to clean centrifuge tubes and 35 mL cold ice wash buffer was added. Centrifuge tubes were gently shaken until the RBC's were dissolved. Samples were centrifuged at 8 C. and 1500g, 10 min, with slow brake-settings (same centrifuge as above). 33 mL supernatant was discarded, 33 mL wash buffer added and the pellet was dissolved by gentle shaking. The centrifugation and cleaning steps were repeated twice. After the last centrifugation as much supernatant as possible (without disturbing the pellet) was removed.

[0119] Preparation of RBC Cytosol

[0120] RBC pellet was dissolved in 20 mL ice cold lysis buffer and shaken 30 min at 4 C. Samples were centrifuged at 25 000g, 15 min, at 8 C. with slow brake-settings (Beckman Coulter, Avanti J-20 XP, JA17 or 25.50 rotor). 15 mL supernatant (collected from middle of fraction) was transferred to clean Centrifuge tubes. The centrifugation step was repeated once. 1.5 mL supernatant was aliquoted to four 2.0 mL micro tubes (2.0 mL Clear MAXYclear, Axygen).

[0121] Protein Concentration Measurements

[0122] All protein concentration measurements were performed using the Bradford assay. Coomassie Protein Assay reagent (Thermo Fischer) was prepared and used as described by the manufacturer's instructions (Thermo Fischer, art. no. #23200). Following a 15 min incubation the absorbance at 595 nm for bovine serum albumin standard samples (ranging from 100-600 ng/l) and RBC cytosol samples were measured in triplicate on a Multiskan GO plate reader (Thermo Fischer). Concentration of samples was calculated from the linear fit of the standard curve.

[0123] Removal of Hemoglobin

[0124] Hemoglobin depletion was performed using HemoVoid resin, buffers and filter tubes as described by the manufacturer (Biotech Support Group) using a standard bench-top centrifuge. To filter tubes containing 15 mg HemoVoid resin 200 pl, HemoVoid binding buffer (HVBB) was added and tubes were mixed end-over-end for 5 min at room temperature. Tubes were centrifuged 5 min at 825g to remove HVBB and the wash was thereafter repeated one more time. To filter tubes 300 l HVBB was added together with 300 l RBC cytosol sample and tubes were mixed end-over-end for 15 min at room temperature. Tubes were centrifuged 8 min in room temperature, at 6,000 rpm. The flow-through was discarded and sample loading was repeated one more time using 300 l additional RBC cytosol sample in the same way. HemoVoid resin was washed with 500 l HemoVoid wash buffer (HVWB) for 5 min at room temperature on an end-over-end rotator, followed by centrifugation as above and discarding of flow-through. The resin was similarly repeated two additional times before performing sample elution by addition of 150 l HemoVoid elution buffer (HVEB), end-over-end mixing for 15 min at room temperature and finally centrifugation for 4 min in room temperature at 10,000 rpm.

[0125] Trypsin Digestion and Clean-Up

[0126] Equivalent amounts of samples were transferred to 0.8 mL 96-well format plates (Waters) for trypsin digestion and C18 cleanup. Assuming an average protein MW of 30 kDa for the hemoglobin depleted RBCc fractions, pre-digest internal peptide standards solubilized in Milli-Q grade (MQ)-water were added to samples at a final molar ratio of 1:100. Samples were then first reduced for 60 min at 60 C. in 5.1 mM dithiothreitol (Bio-Rad) and thereafter alkylated for 30 min at 22 C. by addition of iodoacetamide (Bio-Rad) to a final concentration of 25 mM. Trypsin Gold (Promega) was added to a final ratio of 0.54 U per pg protein sample (approximately a 1:30 (w/w) ratio) and samples were incubated for 16 h at 37 C. In order to stop the reaction and acidify samples (to a final pH of 3-4) for C18 binding acetonitrile and trifluoroacetic acid (TFA) were added to final concentrations of 5% (v/v) acetonitrile and 1% (v/v) TFA.

[0127] Clean-up of trypsin digest samples was performed in 96-well format at room temperature with a Positive Pressure 96 unit (Waters) using 10 mg bed weight Hypersep C18 plates (Thermo Fischer) according to the manufacturer's instructions. In brief, the resin was washed twice with 500 l 50% (v/v) methanol and thereafter equilibrated twice with 5% (v/v), 0.5% (v/v) TFA. Samples were applied to the resin and the flow-through was after collection applied again to the resin. The resin was washed twice with 500 l 5% (v/v), 0.5% (v/v) TFA and tryptic peptides were finally eluted into new 0.8 mL 96-well plates (Waters) in 100 l 70% (v/v) acetonitrile. Acetonitrile was evaporated in a SpeedVac and dried samples were solubilized for 2 h at room temperature in 100 l MQ-water before storage at 80 C.

[0128] Nano LC-MSMS Analysis

[0129] Samples to be analysed were transferred to LC vials (1 mL, TruView LCMS Certified Clear glass 1232 mm screw neck total recovery vial with cap and preslit PTFE/silicone septa, Prod. No. 186005663CV, Waters). Samples used for analysis were thawed and diluted to the desired concentration with MQ-water. Internal retention time standards (iRT, Biognosys), pre-LC internal standard and TFA were added to final concentrations of 8 fmol/l, 8 fmol/l and 0.1% (v/v) respectively. 5 L sample, corresponding to 50-400 ng peptide sample, was injected on a nanoACQUITY UltraPerformance UPLC (Waters), with full loop injection at a flow rate of 0.33 L/min. Peptides were first bound to a nanoEase M/Z Symmetry C18 Trap Column (100, 5 m, 180 m20mm, 2G, #186008821, Waters) and then separated on a nanoEase MZ HSS T3 column (15K psi, 100 , 1.8m, 75 m250mm, #186008818, Waters) using a column temperature of 40 C. and sample temperature of 8 C. Inorganic phase solvent (solvent A) consisted of 0.1% formic acid (FA) in water, organic phase solvent (solvent B) of 99.9% acetonitrile (w/v), 0.1% (v/v) FA, seal wash of 10% (w/v) acetonitrile, weak wash of 1% (w/v) acetonitrile, 0.1% (v/v) TFA, lock spray solution of 25% (w/v) acetonitrile, 0.1% (v/v) FA, 0.5% (v/v) Leu-Enk, 1.6% (v/v) Glu-Fib. Following an 8.5 minute step at solvent ratio 95% A/5% B, samples were separated on a 60 min gradient from 95% A/5% B to 60% A/40% B. Thereafter a 1.5 min gradient to 15% A/85% B was applied and held for 2.5 minutes before the column was finally washed for 30 mins with 95% A/5% B. Separated peptides were analysed by mass spectrometry using a nano-UPLC Synapt G2Si (Waters). The mass analyser was run in positive resolution mode, analytes were ionized by electrospray ionization (ES) using a mass range of 50-2000 Da, scan time of 0.5 s, sampling cone voltage 30 V. Data acquisition and initial data evaluation were performed using MassLynx software (ver. SCN901, Waters).

[0130] Data Processing and Analysis

[0131] Raw data processing, alignment, peptide identification, quantification and comparative analysis were performed using Progenesis QI for Proteomics (v.3.0.6039.34628, Waters), Proteinlynx Global Server (ver. 3.0.3, Waters) and Skyline daily (ver. 3.7.1.11208). For peptide identification a curated human proteome database was used (Swiss-Prot), acquired from Uniprot (www.uniprot.org) (ver. 10th August 2016) in FASTA format using the following identification parameters: missed cleavages 2, variable modifications carbamidomethylation of cysteine, N-terminal acetylation, oxidation of methionine, peptide tolerance 10 ppm, fragment tolerance 25 ppm, false discovery rate <2%, fragments per peptide 1, fragments per protein 3. Common contaminants and internal standards were added to the database. Additional multivariate statistical analysis was performed using SIMCATM (Umetrics AB).

Results

[0132] Identification of Biomarkers

[0133] The difference in the level of individual peptides in tryptic peptide maps in samples obtained from individuals having received autologous blood transfusion were compared to the level of the same specific peptides in reference tryptic peptide maps in samples obtained from a reference population of non-transfused control subjects.

[0134] Peptides with significant difference in levels are listed in Table 2 and the corresponding proteins are listed in Table 3.

[0135] These peptides, and the corresponding proteins, can be used as biomarkers in methods for the detection of autologous blood doping.

TABLE-US-00002 TABLE2 Specificpeptides Retention Retentiontime PeptideVariablemodifications time(min) window(min) Mass ([position]description) SEQIDNo. 69.17 0.41 1541.82 GAYIYNALIEFIR 1 57.33 0.60 1583.73 SIQFVDWCPTGFK 2 [8]CarbamidomethylC 48.99 0.37 1539.71 WELNSGDGAFYGPK 3 60.67 0.24 2669.33 VNNVVWDLDRPLEEDCTLELLK 4 [16]CarbamidomethylC 36.00 0.30 751.30 QAEEEF 5 58.54 0.22 1805.96 NAQLAQYNFILVVGEK 6 45.52 0.31 2319.09 AAAASAAEAGIATTGTEDSDDALLK 7 37.98 0.46 1371.74 AIADTGANVVVTGGK 8 56.41 0.24 1695.83 NSSYFVEWIPNNVK 9 41.61 0.33 1157.58 IDIDPEETVK 10 22.40 0.29 1382.67 DSSREASTSNSSR 11 52.88 0.42 1142.62 LAVNMVPFPR 12 22.86 0.41 940.52 TLPKSMHK 13 60.07 0.36 1386.77 ITVNEVELLVMK 14 62.88 0.27 2408.18 FDGALNVDLTEFQTNLVPYPR 15 69.16 0.39 1598.84 TPLLLMLGQEDRR 16 [1]AcetylN-terminal [6]OxidationM 28.57 0.33 1439.60 SYNKDLESAEER 17 37.47 0.34 1643.78 ITCLCQVPQNAANR 18 [3]CarbamidomethylC [5]CarbamidomethylC 48.05 0.33 1323.77 AVAVVVDPIQSVK 19 41.78 0.24 2156.08 TVGTPIASVPGSTNTGTVPGSEK 20 37.93 0.47 1465.68 QDEWIKFDDDK 21 [1]Methylation [6]Methylation 41.59 0.63 1615.79 QPAENVNQYLTDPK 22 42.62 0.45 812.51 NIILGGVK 23 36.51 0.85 1664.74 DTSQSDKDLDDALDK 24 36.51 0.53 1664.74 DTSQSDKDLDDALDK 24 64.39 0.36 1597.91 VWINTSDIILVGLR 25 22.50 0.31 837.36 YNADEAR 26 22.76 0.41 1408.61 EFQSPDEEMKK 27 [1]AcetylN-terminal 42.00 0.73 730.40 DAAITLK 28 39.33 0.43 1496.77 QTLQSEQPLQVAR 29 61.66 0.66 2023.10 DTYARWLPLGLGLNHLGK 30 33.77 0.27 659.34 VAEWR 31 43.88 0.58 1867.90 LALCLCMINFYHGGLK 32 [6]CarbamidomethylC [7]OxidationM 33.77 0.41 730.38 VAAEWR 33 40.96 0.30 1587.75 AVEAAELCLEQNNK 34 [8]CarbamidomethylC 62.70 0.85 1248.58 MEGPLSVFGDR 35 [1]AcetylN-terminal 45.79 0.41 1407.67 AVFDETYPDPVR 36 23.22 0.41 1564.64 AAEDDEDDDVDTKK 37 32.01 0.61 1120.54 GYLPSHYER 38 29.73 0.31 740.38 EFFNGK 39 53.60 0.29 1606.81 GFQEVVTPNIFNSR 40 31.83 0.53 1479.62 QCNRHYCWEK 41 [2]CarbamidomethylC [7]CarbamidomethylC 22.48 0.38 1223.65 VAAAETAKHQAK 42 36.27 0.15 1498.76 ARDAAEFELFFR 43 [2]Methylation [3]Methylation 33.79 0.26 931.46 QDADSLQR 44 51.79 0.42 1135.59 LGIRFCTNR 45 [6]CarbamidomethylC 23.22 0.38 1564.63 AAEDDEDDDVDTKK 37 22.76 0.34 1465.63 ETDSSSASAATPSKK 46 28.91 0.46 1407.61 SNTAGSQSQVETEA 47 38.30 0.29 1278.61 EDGQEYAQVIK 48 23.22 0.43 1621.66 RMTGSEFDFEEMK 49 [12]OxidationM 23.61 0.34 1092.46 MGHFTEEDK 50 22.21 0.26 1165.55 NEQESAVHPR 51 34.01 0.46 1733.85 TQTPPVSPAPQPTEER 52 40.62 0.22 628.39 LLDLR 53 30.39 0.41 959.47 HLTGEFEK 54 59.88 0.44 2320.15 KYFHAQLQLEQLQEENFR 55 27.65 0.24 69935 SMHLGR 56 33.77 0.21 659.34 VAEWR 31 27.05 0.44 1288.62 QLEDELAAMQK 57 [10]Methylation 33.22 0.34 1964.86 CGNCGPGYSTPLEAMKGPR 58 [1]Methylation [13]Methylation 23.23 0.34 1621.65 RMTGSEFDFEEMK 49 [12]OxidationM 49.06 0.33 936.45 SVQTFADK 59 [1]AcetylN-terminal 39.69 0.24 820.43 FVVDVDK 60 23.47 0.41 1186.60 REQQPSVTSR 61 28.99 0.46 1149.48 MNPNCARCGK 62 [5]CarbamidomethylC 46.26 0.31 1919.95 QVMVVPVGPTCDEYAQK 63 [11]CarbamidomethylC 44.61 0.41 1848.88 QYTSPEEIDAQLQAEK 64 34.49 0.22 898.44 VLEAGDAQP 65 36.46 0.26 1770.78 AEEADHEVLDQKEMK 66 32.78 0.49 1964.86 MCKQDPSVLHTEEMR 67 [1]GlycationN-Terminal 39.60 0.27 1551.69 YDSNSGGEREIQR 68 [1]AcetylN-terminal 46.49 0.33 1894.94 ELVFKEDGQEYAQVIK 69 54.36 0.26 3047.48 SLGSLPGSVVEANPNQRDPPLWDEIDSR 70 61.09 0.21 2466.09 MWDVSTGMCLMTLVGHDNWVR 71 [8]OxidationM 25.40 0.22 1024.52 VAEIEHAEK 72 33.53 0.27 1883.84 GDYHRYLAEFATGNDR 73 64.80 0.39 2460.13 DQELYFFHELSPGSCFFLPK 74 [15]CarbamidomethylC 37.94 0.56 1228.64 IHPTSVISGYR 75 45.31 0.60 869.53 LAVEAVLR 76 49.01 0.39 940.47 SLDNFFAK 77 59.70 0.37 1309.76 GGPNIITLADIVK 78

TABLE-US-00003 TABLE3 ProteinscorrespondingtothespecificpeptidesidentifiedinTable2 PeptideVariablemodifications SEQ ([position]description) IDNo. Protein Description GAYIYNALIEFIR 1 P26639|SYTC_HUMAN Threonine-tRNAligase,cytoplasmic SIQFVDWCPTGFK 2 P68363|TBA1B_HUMAN Tubulinalpha-1Bchain [8]CarbamidomethylC WELNSGDGAFYGPK 3 P26639|SYTC_HUMAN Threonine-tRNAligase,cytoplasmic VNNVVWDLDRPLEEDCTLELLK 4 P26639|SYTC_HUMAN Threonine-tRNAligase,cytoplasmic [16]CarbamidomethylC QAEEEF 5 P26639|SYTC_HUMAN Threonine-tRNAligase,cytoplasmic NAQLAQYNFILVVGEK 6 P26639|SYTC_HUMAN Threonine-tRNAligase,cytoplasmic AAAASAAEAGIATTGTEDSDDALLK 7 P55036|PSMD4_HUMAN 26Sproteasomenon-ATPaseregulatory subunit4 AIADTGANVVVTGGK 8 P50990- Isoform2ofT-complexprotein1 2|TCPQ_HUMAN subunittheta NSSYFVEWIPNNVK 9 P68371|TBB4B_HUMAN Tubulinbeta-4Bchain IDIDPEETVK 10 P54727|RD23B_HUMAN UVexcisionrepairproteinRAD23 homologB DSSREASTSNSSR 11 P78318|IGBP1_HUMAN Immunoglobulin-bindingprotein1 LAVNMVPFPR 12 P68371|TBB4B_HUMAN Tubulinbeta-4Bchain TLPKSMHK 13 Q96NW7- Isoform2ofLeucine-richrepeat- 2|LRRC7_HUMAN containingprotein7 ITVNEVELLVMK 14 Q9UNM6|PSD13_HUMAN 26Sproteasomenon-ATPaseregulatory subunit13 FDGALNVDLTEFQTNLVPYPR 15 P68363|TBA1B_HUMAN Tubulinalpha-1Bchain TPLLLMLGQEDRR 16 P13798|ACPH_HUMAN Acylamino-acid-releasingenzyme [1]AcetylN-terminal [6]OxidationM SYNKDLESAEER 17 O43242|PSMD3_HUMAN 26Sproteasomenon-ATPaseregulatory subuni3 ITCLCQVPQNAANR 18 P49558|SYAC_HUMAN Alanine-tRNAligase,cytoplasmic [3]CarbamidomethylC [5]CarbamidomethylC AVAVVVDPIQSVK 19 O00487|PSDE_HUMAN 26Sproteasomenon-ATPaseregulatory subunit14 TVGTPIASVPGSTNTGTVPGSEK 20 Q99460- Isoform2of26Sproteasomenon-ATPase 2|PSMD1_HUMAN regulatorysubunit1 QDEWIKFDDDK 21 P54579|UBP14_HUMAN Ubiquitincarboxyl-terminalhydrolase14 [1]Methylation [6]Methylation QPAENVNQYLTDPK 22 P22314- Isoform2ofUniquitin-likemodifier- 2|UBA1_HUMAN activatingenzyme1 NIILGGVK 23 P22314|UBA1_HUMAN Ubiquitin-likemodifier-activating enzyme1 DTSQSDKDLDDALDK 24 P20810|ICAL_HUMAN Isoform3ofCalpastatin VWINTSDIILVGLR 25 O14602|IF1AY_HUMAN Eukaryotictranslationinitiationfactor 1A,Y-chromosomal YNADEAR 26 O14602|IF1AY_HUMAN Eukaryotictranslationinitiationfactor 1A,Y-chromosomal EFQSPDEEMKK 27 O75533|SF3B1_HUMAN Splicingfactor3Bsubunit1 [1]AcetylN-terminal DAAITLK 28 Q07960|RHG01_HUMAN RhoGTPase-activatingprotein1 QTLQSEQPLQVAR 29 P35998|PRS7_HUMAN 26Sproteaseregulatorysubunit7 DTYARWLPLGLGLNHLGK 30 Q13200|PSMD2_HUMAN 26Sproteasenon-ATPaseregulatory subunit2 VAEWR 31 Q9Y230|RUVB2_HUMAN RubB-like2 LALCLCMINFYGHHLK 32 Q15042|RB3BP_HUMAN Rab3GTPase-activatingproteincatalytic [6]CarbamidomethylC subunit [7]OxidationM VAAEWR 33 Q02218- Isoform2of2-oxoglutarate 2|ODO1_HUMAN dehydrogenase,mitochondrial AVEAAELCLEQNNK 34 P10746|HEM4_HUMAN Uroporphyrinogen-IIIsynthase [8]CarbamidomethylC MEGPLSVFGDR 35 P17987|TCPA_HUMAN T-complexprotein1subunitalpha [1]AcetylN-terminal AVFDETYPDPVR 36 P49588|SYAC_HUMAN Alanine-tRNAligase,cytoplasmic AAEDDEDDDVDTKK 37 P06454- Isoform2ofProthymosinalpha 2|PTMA_HUMAN GYLPSHYER 38 Q6P2Q9|PRP8_HUMAN Pre-mRNA-processing-splicingfactor8 EFFNGK 39 P11021|GRP78_HUMAN 78kDaglucose-regulatedprotein GFQEVVTPNIFNSR 40 P26639|SYTC_HUMAN Threonine-tRNAligase,cytoplasmic QCNRHYCWEK 41 Q9P0U4- Isoform2ofCXXC-typezincfiner [2]CarbamidomethylC 2|CXXC1_HUMAN protein1 [7]CarbamidomethylC VAAAETAKHQAK 42 P11532|DMD_HUMAN Dystrophin ARDAAEFELFFR 43 Q6XQN6|PNCB|HUMAN Nicotinatephosphoribosyltransferase [2]Methylation [3]Methylation QDADSLQR 44 P14324|FPPS_HUMAN Farnesylpyrophosphatesynthase LGIRFCTNR 45 [13798|ACPH_HUMAN Acylamino-acid-releasingenzyme4 [6]CarbamidomethylC ETDSSSASAATPSKK 46 P54578- Isoform2ofUbiquitincarboxy-terminal 2|UBP14_HUMAN hydrolase14 SNTASGSQSQVETEA 47 Q02790|FKVP4_HUMAN Peptidyl-prolylcis-transisomerase FKBP4 EDGQEYAQVIK 48 O14602|IF1AY_HUMAN Eukaryotictranslationinitiationfactor 1A,Y-chromosomal RMTGSEFDFEEMK 49 P50396|GDIB_HUMAN RabGDPdissociationinhibitorbeta [12]OxidationM MGHFTEEDK 50 P69891|HBG1_HUMAN Hemoglobinsubunitgamma-1 NEQESAVHPR 51 Q9UJU6|DBNL_HUMAN Drebrin-likeprotein TQTPPVSPAPQPTEER 52 Q14247|SRC8_HUMAN Srcsubstratecortactin LLDLR 53 Q8IZT6|ASPM_HUMAN Abnormalspindle-likemircocephaly- associatedprotein HLTGRFEK 54 P62826|RAN_HUMAN GTP-bindingnuclearproteinRan KYFHAQLQLEQLQEENFR 55 Q9UJC3|HOOK1_HUMAN ProteinHookhomolog1 SMHLGR 56 Q9Y570|PPME1_HUMAN Proteinphosphatasemethyltransferase1 QLEDEALAAMQK 57 P06753|TPM3_HUMAN Tropomyosinalpha-3chain [10]Methylation [1]Methylation 58 Q13228|SBP1_HUMAN Selenium-bindingprotein1 [13]Methylation SVQTFADK 58 P47756- Isoform2ofF-actin-cappingprotein [1]AcetylN-terminal 2|CAPZB_HUMAN subunitbeta FVVDVDK 60 P62195|PRS8_HUMAN Isoform2of26Sproteaseregulatory subunit8 REQQPSVTSR 61 P25686- Isoform2ofDanJhomologsubfamilyB 2|DNJB2_HUMAN member2 MNPNCARCGK 62 Q14847|LASP1_HUMAN LIMandSH3domainprotein1 [5]CarbamidomethylC QVMVVPVGPTCDEYAQK 63 P26639|SYTC_HUMAN Threonine-tRNAligase,cytoplasmic [11]CarbamidomethylC QYTSPEEIDAQLQAEK 64 Q13442|HAP28_HUMAN 28kDaheat-andacid-stable phosphoprotein VLEAGDAQP 65 Q5JRK9|GGEE3_HUMAN PutativeGantigenfamilyEmember3 AEEADHEVLDQKEMK 66 Q`4789- Isoform4ofGolginsubfamilyBmember1 4|GOGB1_HUMAN MCKQDPSVLHTEEMR 67 Q8NFI4|F10A5_HUMAN PutativeproteinFAM10A51 [1]GlycationN-terminal YDSNSGGEREIQR 68 P62191|PRS4_HUMAN 26Sproteaseregulatorysubunit4 [1]AcetylN-terminal ELVFKEDGQEYAQVIK 69 O14602|IF1AY_HUMAN Eukaryotictranslationinitiationfactor 1A,Y-chromosomal SLGSLPGSVVEANPNQRDPPLWDEIDSR 70 Q16864- Isoform2ofV-typeprotonATPase 2|VATE_HUMAN subunitF MWDVSTGMCLMTLVGHDNWVR 71 P43043|LIS1_HUMAN Platelet-activatingfactoracetyl- [8]OxidationM hydrolaseIBsubunitalpha VAEIEHAEK 72 P78371|TCPB_HUMAN T-complexprotein1subunitbeta GDYHRYLAEFATGNDR 73 P62258- IsoformSVof14-3-3proteinepsilon 2|1433E_HUMAN DQELYFFHELSPGSCFFLPK 74 P26639|SYTC_HUMAN Threonine-tRNAligase,cytoplasmic [15]CarbamidomethylC IHPTSVISGYR 75 P17987|TCPA_HUMAN T-complexprotein1subunitalpha LAVEAVLR 76 P78371|TCPB_HUMAN T-complexprotein1subunitbeta SLDNFFAK 77 Q9UKY7|CDV3_HUMAN ProteinCDV3homolog GGPNIITLADIVK 78 P68400|CSK21_HUMAN CaseinkinaseIIsubunitalpha