FLEXIBLE PAPER-BASED SURFACE-ENHANCED RAMAN SCATTERING SUBSTRATE AND METHOD FOR PREPARING SAME

Abstract

Disclosed are a flexible paper-based surface-enhanced Raman scattering (SERS) substrate and a method of preparing the same, which relate to detection of trace organics. This invention deposits a film of silver nanoparticles on a carrier such as filter paper by oblique-angle deposition to prepare the SERS substrate, which can be used for the detection of trace organics. Silver nanoparticles are closely arranged on the flexible paper, allowing the substrate for good SERS effect due to the interparticle coupling effect. Moreover, compared to the traditional rigid and brittle substrates, the substrate prepared herein can be readily used in the detection of residual organics on complex surfaces in various liquid or solid conditions by dipping, dropwise adding or wiping due to the use of a flexible and absorbent carrier.

Claims

1. A flexible paper-based surface-enhanced Raman scattering substrate, comprising: a layer of silver nanoparticles; and a fiber paper; wherein the layer of silver nanoparticles is deposited on and tightly bound to the fiber paper by electron beam evaporation oblique-angle deposition.

2. The substrate of claim 1, wherein the layer of silver nanoparticles deposited on the fiber paper has a thickness corresponding to a crystal oscillator parameter range of 500-1300 nm.

3. A method of preparing the flexible paper-based surface-enhanced Raman scattering substrate of claim 1, comprising: depositing silver on the fiber paper by electron beam evaporation oblique-angle deposition while rotating the fiber paper to produce the substrate.

4. A method of preparing the flexible paper-based surface-enhanced Raman scattering substrate of claim 2, comprising: depositing silver on the fiber paper by electron beam evaporation oblique-angle deposition while rotating the fiber paper to produce the substrate.

5. The method of claim 3, further comprising: fixing the fiber paper flatly on a holder of an electron beam evaporation deposition system at room temperature; choosing the silver as target material, and vacuumizing a reaction chamber of the electron beam evaporation deposition system to 310.sup.5310.sup.4 Pa; adjusting an angle between a normal direction of the holder and an incident direction of an evaporated beam to 85-88; and rotating the fiber paper at 2-10 rpm; and depositing the layer of silver nanoparticles on the fiber paper, wherein the layer of silver nanoparticles has a thickness corresponding to a crystal oscillator parameter range of 500-1300 nm.

6. The method of claim 4, further comprising: fixing the fiber paper flatly on a holder of an electron beam evaporation deposition system at room temperature; choosing the silver as target material, and vacuumizing a chamber of the electron beam evaporation deposition system to 310.sup.5310.sup.4 Pa; adjusting an angle between a normal direction of the holder and an incident direction of an evaporated beam to 85-88; and rotating the fiber paper at 2-10 rpm; and depositing the layer of silver nanoparticles on the fiber paper, wherein the layer of silver nanoparticles has a thickness corresponding to a crystal oscillator parameter range of 500-1300 nm.

7. The method of claim 3, wherein the fiber paper is filter paper, absorbent paper or printing paper.

8. The method of claim 4, wherein the fiber paper is filter paper, absorbent paper or printing paper.

9. The method of claim 3, wherein the fiber paper is rotated at 2-10 rpm.

10. The method of claim 4, wherein the fiber paper is rotated at 2-10 rpm.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0027] FIG. 1a is a scanning electron microscope (SEM) image of a flexible paper-based surface-enhanced Raman scattering (SERS) substrate prepared in Example 1 of the invention.

[0028] FIG. 1b is a SERS spectrum showing the detection of a 10.sup.7 mol/L 4-mercaptobenzoic acid (4-MBA) solution using the flexible SERS substrate prepared in Example 1 of the invention.

[0029] FIG. 2 is a SERS spectrum showing the detection of trace rhodamine 6G (R6G) solid residue using a flexible SERS substrate prepared in Example 2 of the invention, where a 10.sup.6 mol/L R6G solution is dropped on a clean glass slide, and the glass slide is dried in ambient air and wiped with the pre-moistened flexible SERS substrate, and then the flexible SERS substrate is transferred to a portable Raman spectrometer for analysis.

[0030] FIG. 3 is a SERS spectrum showing the detection of trace 4-mercaptobenzoic acid (4-MBA) using a flexible SERS substrate prepared in Example 3 of the invention, where the surface of a cleaned apple is smeared with a 10.sup.5 mol/L 4-MBA solution, dried and wiped with the flexible SRES substrate, and then the SRES substrate is subjected to Raman spectroscopic measurement.

DETAILED DESCRIPTION OF EMBODIMENTS

[0031] This invention prepares a flexible surface-enhanced Raman scattering (SERS) substrate by depositing silver nanoparticles on a fiber paper through electron beam evaporation oblique-angle deposition, where the silver nanoparticles are arranged closely, allowing for excellent SERS performance. Thus, the flexible SERS substrate can be applied in the detection of trace organics. Moreover, due to the introduction of a flexible fiber paper which has good adsorption to liquid, the SERS substrate can be conveniently used in the detection of trace organics in liquids by dipping compared to the rigid SERS substrate. The residual liquids and solids on complex curved surfaces can also be rapidly detected and analyzed after wiped with the flexible SERS substrate.

[0032] The invention will be further described below with reference to the accompanying drawings and embodiments. These embodiments are illustrative of the invention and are not intended to limit the invention.

[0033] FIG. 1a is a scanning electron microscope (SEM) image of a flexible paper-based SERS substrate prepared in Example 1 of the invention, from which it can be clearly seen that silver nanoparticles are evenly distributed on the fiber paper. FIG. 1b is a SERS spectrum showing the detection of a 10.sup.7 mol/L 4-mercaptobenzoic acid (4-MBA) solution using the flexible SERS substrate prepared in Example 1, in which characteristic Raman scattering peaks of 4-MBA are clearly observed.

[0034] FIG. 2 is a Raman spectrum showing the detection of trace rhodamine 6G (R6G) using a flexible SERS substrate prepared in Example 2 of the invention, where a 10.sup.6 mol/L R6G solution is dropped on a clean glass slide, dried and wiped with the pre-moistened flexible SERS substrate; and then the flexible SERS substrate is transferred to a Raman spectrometer for analysis. In the spectrum, characteristic Raman scattering peaks of R6G are marked with corresponding wave number such as 1362 cm.sup.1 and 1509 cm.sup.1.

[0035] FIG. 3 is a SERS spectrum showing the detection of trace 4-MBA using a flexible SERS substrate prepared in Example 3 of the invention, where a surface of a cleaned apple is smeared with a 10.sup.5 mol/L 4-MBA solution, dried and wiped with the flexible SERS substrate, and then the SERS substrate is subjected to Raman spectroscopic measurement. In the SERS spectrum, the solid line represents the test result of the flexible SERS substrate after wiping 4-MBA. As we can see, the flexible SERS substrate proves to have good performance in the trace detection of residual organics on the curved surfaces.

Example 1

[0036] (1) A filter paper, an absorbent paper or a printing paper, was flatly fixed on a holder of an electron beam evaporation deposition system.

[0037] (2) High-purity silver was employed as target material, and a reaction chamber of the electron beam evaporation deposition system was vacuumized at room temperature to 1104 Pa.

[0038] (3) An angle between a normal direction of the holder and an incident direction of an evaporated beam was adjusted to 86, and the holder rotated at 2 rpm, thereby depositing a film of silver nanoparticles on the fiber paper by oblique-angle deposition to produce the SERS substrate, where the film of silver nanoparticles had a thickness according to a crystal oscillator parameter of 700 nm.

[0039] (4) A 10.sup.7 mol/L 4-MBA solution was prepared.

[0040] (5) The SERS substrate prepared through steps 1-3 was dipped into the 4-MBA solution prepared in step (4) and then transferred for use.

[0041] (6) The SERS substrate after absorbing 4-MBA solution in step (5) was analyzed by Raman spectrometer in which 785 nm laser was used.

[0042] FIG. 1a was a scanning electron microscope (SEM) image clearly showing the fiber paper deposited with the film of silver nanoparticles. The Raman spectroscopic detection results of the 10.sup.7 mol/L 4-MBA solution were shown in FIG. 1b, in which the characteristic Raman scattering peaks of 4-MBA were clearly observed.

Example 2

[0043] (1) A filter paper, an absorbent paper or a printing paper was flatly fixed on a holder of an electron beam evaporation deposition system.

[0044] (2) High-purity silver was chosen as target material, and a reaction chamber of the electron beam evaporation deposition system was vacuumized at the ambient temperature to 310.sup.4 Pa.

[0045] (3) An angle between a normal direction of the holder and an incident direction of an evaporated beam was adjusted to 85, and the holder rotated at 10 rpm, thereby depositing a film of silver nanoparticles on the fiber paper by oblique-angle deposition to produce the SERS substrate, where the film of silver nanoparticles had a thickness according to a crystal oscillator parameter of 1000 nm.

[0046] (4) A 10.sup.6 mol/L R6G solution was prepared.

[0047] (5) 20 L of the R6G solution prepared in step (4) was dropwise added on a clean glass slide and dried naturally.

[0048] (6) The SERS substrate prepared through steps 1-3 was moistened with deionized water, and then wiped the trace R6G residual in step (5).

[0049] (7) The SERS substrate after wiping the trace R6G residual in step (6) was analyzed by Raman spectrometer using 785 nm laser.

[0050] FIG. 2 was the SERS spectrum showing the detection of trace R6G using the flexible SERS substrate by dropwise adding and wiping, in which characteristic Raman scattering peaks of R6G were clearly observed, proving that the SERS substrate prepared herein well realized the detection of the R6G residue solid.

Example 3

[0051] (1) A filter paper, an absorbent paper or a printing paper was flatly fixed on a holder of an electron beam evaporation deposition system.

[0052] (2) High-purity silver was chosen as target material, and a reaction chamber of the electron beam evaporation deposition system was vacuumized at the ambient temperature to 110.sup.4 Pa.

[0053] (3) An angle between a normal direction of the hold and an incident direction of an evaporated beam was adjusted to 87, and the holder rotated at 2 rpm, thereby depositing a film of silver nanoparticles on the fiber paper by oblique-angle deposition to produce the SERS substrate, where the film of silver nanoparticles had a thickness according to a crystal oscillator parameter of 1300 nm.

[0054] (4) A 10.sup.5 mol/L 4-MBA solution was prepared.

[0055] (5) A trace amount of the 4-MBA solution prepared in step (4) was smeared to surfaces of a clean apple and dried.

[0056] (6) The SERS substrate prepared through steps 1-3 was moistened with absolute ethanol, and then wiped the trace 4-MBA in step (5).

[0057] (7) The SERS substrate after wiping the trace 4-MBA in step (6) was analyzed by Raman spectrometer in which 785 nm laser was used.

[0058] FIG. 3 showed the Raman spectroscopic detection results of the trace 4-MBA, in which the characteristic Raman scattering peaks of 4-MBA were clearly observed, proving that trace 4-MBA remained on apple surfaces after the drying treatment. The residues on curved surfaces can be rapidly and effectively detected after wiped with the flexible SERS substrate.

[0059] The technical solutions of this invention are illustrated in detail above with reference to the embodiments. Obviously, these embodiments are not intended to limit the invention. Any changes and replacements made by those skilled in the art without departing from the spirit of the invention should fall within the scope of the invention.