Experimental Device and Experimental Method for Studying Quantitative Relationship between Spawning of Parent Fish and Stimulation of Flow velocity

Abstract

A experimental device is specifically an annular water tank formed by a first arc-shaped water tank, a first special-shaped water tank, a second arc-shaped water tank and a second special-shaped water tank which are sequentially connected head to tail, and the outer perimeter is 70 m to 110 m, wherein the inner walls of both the first special-shaped water tank and the second special-shaped water tank are flat, both of the widths between the outer walls and the inner walls are gradually increased from the two ends to the middle, and water flow pushing equipment which are capable of enabling the maximum water flow velocity in the experimental device to reach a preset value are respectively placed in the first special-shaped water tank and the second special-shaped water tank.

Claims

1-9. (canceled)

10. An experimental device for studying a quantitative relationship between spawning of parent fish and stimulation of flow velocity, wherein the experimental device is specifically an annular water tank formed by a first arc-shaped water tank, a first special-shaped water tank, a second arc-shaped water tank and a second special-shaped water tank which are sequentially connected head to tail, and the outer perimeter is 70 m to 110 m, wherein the inner walls of both the first special-shaped water tank and the second special-shaped water tank are flat, both of the widths between the outer walls and the inner walls are gradually increased from the two ends to the middle, and water flow pushing equipment which are capable of enabling the maximum water flow velocity in the water tank to reach a preset value are respectively placed in the first special-shaped water tank and the second special-shaped water tank; the outer wall of the first special-shaped water tank consists of a first inclined wall, a first straight wall and a second inclined wall which are sequentially connected, the first straight wall is parallel to the inner wall of the first special-shaped water tank, the first inclined wall is connected with the outer wall of the first arc-shaped water tank, and the second inclined wall is connected with the outer wall of the second arc-shaped water tank; and the outer wall of the second special-shaped water tank consists of a third inclined wall, a second straight wall and a fourth inclined wall which are sequentially connected, the second straight wall is parallel to the inner wall of the second special-shaped water tank, the third inclined wall is connected with the outer wall of the first arc-shaped water tank, and the fourth inclined wall is connected with the outer wall of the second arc-shaped water tank.

11. The experimental device for studying a quantitative relationship between spawning of parent fish and stimulation of flow velocity according to claim 10, wherein the outer wall radiuses of both the first arc-shaped water tank and the second arc-shaped water tank are 7 m to 12 m, the widths are 0.5 m to 1.2 m and the depths are 1 m to 2 m; and the lengths of the inner walls of both the first special-shaped water tank and the second special-shaped water tank are 11 m to 20 m, the widths are 1 m to 2.5 m and the depths are 1 m to 3 m.

12. The experimental device for studying a quantitative relationship between spawning of parent fish and stimulation of flow velocity according to claim 11, wherein the included angle 1 between the first inclined wall and the inner wall of the first special-shaped water tank satisfies 5130; the included angle 2 between the second inclined wall and the inner wall of the first special-shaped water tank satisfies 5230; the included angle 3 between the third inclined wall and the inner wall of the second special-shaped water tank satisfies 5330; and the included angle 4 between the fourth inclined wall and the inner wall of the second special-shaped water tank satisfies 5430.

13. The experimental device for studying a quantitative relationship between spawning of parent fish and stimulation of flow velocity according to claim 10, wherein a first baffle is arranged along a length direction in the first special-shaped water tank, and the two ends of the first baffle are respectively connected to the outer wall of the first special-shaped water tank by adoption of fish stopping nets; and a second baffle is arranged along a length direction in the second special-shaped water tank, and the two ends of the second baffle are respectively connected to the outer wall of the second special-shaped water tank by adoption of fish stopping nets.

14. The experimental device for studying a quantitative relationship between spawning of parent fish and stimulation of flow velocity according to claim 13, wherein a first flow pusher is placed in an area surrounded by the first baffle, the outer wall of the first special-shaped water tank and the fish stopping nets, and a second flow pusher is placed in an area surrounded by the second baffle, the outer wall of the second special-shaped water tank and the fish stopping nets.

15. The experimental device for studying a quantitative relationship between spawning of parent fish and stimulation of flow velocity according to claim 14, wherein a first submersible pump is arranged at the junction of the first special-shaped water tank and the first arc-shaped water tank, a second submersible pump is arranged at the junction of the first special-shaped water tank and the second arc-shaped water tank, a third submersible pump is arranged at the junction of the second special-shaped water tank and the second arc-shaped water tank, a fourth submersible pump is arranged at the junction of the second special-shaped water tank and the first arc-shaped water tank, and the submersible pumps and the flow pushers are the water flow pushing equipment.

16. An experimental method for studying a quantitative relationship between spawning of parent fish and stimulation of flow velocity, wherein the method performs studying by adopting the experimental device according to claim 1, and specifically comprises: (1) injecting water into the experimental device to enable the water level in the tank to meet an experimental requirement, and removing impurities and harmful substances in the water; (2) transferring parent fish samples into the experimental device to adapt for 6 hours or more; (3) in a range of velocities suitable for the parent fish to survive, alternately selecting a plurality of velocity respectively as maximum flow velocity for each working condition; (4) according to set working conditions, starting water flow pushing equipment to enable the maximum flow velocity of a water flow to reach a preset value, executing for 2 hours to 2 days under each working condition, respectively recording the spawning situation of the parent fish under each working condition, simultaneously monitoring water quality and parent fish behaviors in real time, cleaning in time or changing water when the water quality is lower than a preset value, stopping the water flow pushing equipment to suspend the experiment when the parent fish are exhausted, and continuing the experiment after the parent fish renew physical strength; and (5) performing sorting to obtain the spawning situation of the parent fish under the stimulation of maximum flow velocity under each working condition.

17. The experimental method for studying a quantitative relationship between spawning of parent fish and stimulation of flow velocity according to claim 16, wherein the total number of the working conditions is four, and the maximum flow velocity under each working condition and combinations of water flow pushing equipment that need to be started in order to reach the maximum flow velocity are specifically shown in the following table: TABLE-US-00004 Working Maximum flow condition velocity Combination way of water flow pushing equipment 1 1.0 m/s First submersible pump + third submersible pump or second submersible pump + fourth submersible pump 2 1.2 m/s First submersible pump + third submersible pump + first flow pusher + second flow pusher or second submersible pump + fourth submersible pump + first flow pusher + second flow pusher 3 1.4 m/s First submersible pump + second submersible pump + third submersible pump + fourth submersible pump 4 1.6 m/s First submersible pump + second submersible pump + third submersible pump + fourth submersible pump + first flow pusher + second flow pusher

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0022] FIG. 1 is a top structural schematic view of an experimental device in the present invention.

[0023] FIG. 2 is cross-sectional views of a-a and b-b in FIG. 1.

[0024] FIG. 3 is a schematic view of flow field zoning of the experimental device in the present invention.

[0025] FIG. 4 is an actual structural view of the experimental device in the present invention.

[0026] FIG. 5 is a schematic view of parent fish.

[0027] FIG. 6 is a schematic view of results of a first group of experiments.

[0028] FIG. 7 is a schematic view of results of a second group of experiments.

DETAILED DESCRIPTION OF THE INVENTION

[0029] FIG. 1 illustrates one embodiment of an experimental device provided by the present invention. The experimental device is specifically an annular water tank formed by a first arc-shaped water tank 100, a first special-shaped water tank 200, a second arc-shaped water tank 300 and a second special-shaped water tank 400 which are sequentially connected head to tail, the radiuses R of the outer walls of the first arc-shaped water tank 100 and the second arc-shaped water tank 300 are 7 m to 12 m, the widths are 0.5 m to 1.2 m and the depth are 1 m to 2 m; the lengths L of the inner walls of the first special-shaped water tank 200 and the second special-shaped water tank 400 are 11 m to 20 m, the widths are 1 m to 2.5 m, the depths are 1 m to 3 m, and the outer perimeter of the annular water tank is 70 m to 110 m. The annular water tank may be designed symmetrically or asymmetrically, that is, the perimeters of the first arc-shaped water tank 100 and the second arc-shaped water tank 300 are not the same, or the perimeters of the first special-shaped water tank 200 and the second special-shaped water tank 400 are not the same, both of which can realize the complex flow field. However, the symmetrical design has a better effect, and control and experimental studies can be better performed. The following description is made by adoption of the symmetrical design as an example.

[0030] As illustrated in FIG. 1, the inner walls of both the first special-shaped water tank 200 and the second special-shaped water tank 400 are flat, and both of the widths between the outer walls and the inner walls are gradually increased from the two ends to the middle, which enables the outer walls of the first special-shaped water tank 200 and the second special-shaped water tank 400 to be roughly isosceles trapezoidal. Specifically, the outer wall of the first special-shaped water tank 200 consists of a first inclined wall 201, a first straight wall 202 and a second inclined wall 203 which are sequentially connected, the first straight wall 202 is parallel to the inner wall of the first special-shaped water tank 200, the first inclined wall 201 is connected with the outer wall of the first arc-shaped water tank 100, and the second inclined wall 203 is connected with the outer wall of the second arc-shaped water tank 300, wherein a tangent connection is most preferably. The second special-shaped water tank 400 and the first special-shaped water tank 200 are symmetrically designed. Understandably, the outer walls of the first special-shaped water tank 200 and the second special-shaped water tank 400 may also be isosceles triangular, i.e., there is no first straight wall and no second straight wall.

[0031] Water flow pushing equipment that can enable the maximum flow velocity to reach a preset value are also placed in the first special-shaped water tank 200 and the second special-shaped water tank 400, which may be a combination of flow pushers and submersible pumps. As illustrated in FIG. 1, a first baffle 204 is arranged along a length direction in the first special-shaped water tank 200, the two ends of the first baffle 204 are respectively connected to the outer wall of the first special-shaped water tank 200 by adoption of fish stopping nets 205, and a first flow pusher 501 is placed in a surrounded area; and a second baffle 404 is arranged along a length direction in the second special-shaped water tank 400, the two ends of the second baffle 404 are respectively connected to the outer wall of the second special-shaped water tank 400 by adoption of fish stopping nets 405, a second flow pusher 502 is placed in a surrounded area, and the depth of the bottom of the pusher is about 1 m deeper than that of the outside of the baffle, as illustrated in FIG. 2. Herein, the first baffle 204 and the second baffle 404 may be cement walls or finished plates, and each flow pusher has blades and may hurt fish during rotation. Therefore, the fish stopping nets and the baffles are adopted to for separation, the length l of the first baffle satisfies l=k*L, and L represents the length of the inner wall of the first special-shaped water tank, k=0.6-0.9; the length of the second baffle is equal to the length of the first baffle, and as verified by simulation analysis and experiments, the baffle with this length can provide a complex flow field more suitable for studies on spawning. In addition, if the flow pushers do not reach the preset maximum flow velocity, submersible pumps may also be additionally arranged. Specifically, a first submersible pump 601 is arranged at the junction of the first special-shaped water tank 200 and the first arc-shaped water tank 100, a second submersible pump 602 is arranged at the junction of the first special-shaped water tank 200 and the second arc-shaped water tank 300, a third submersible pump 603 is arranged at the junction of the second special-shaped water tank 400 and the first arc-shaped water tank 100, and a fourth submersible pump 604 is arranged at the junction of the second special-shaped water tank 400 and the first arc-shaped water tank 100. The submersible pumps have no blades and thus do not injure the fish. It should be noted that the baffles and fish stopping nets may be removed if water flow pushing equipment that does not injure the fish is placed in the special-shaped water tanks.

[0032] The included angle 1 between the first inclined wall 201 and the inner wall of the first special-shaped water tank 200 satisfies 5130; the included angle 2 between the second inclined wall 203 and the inner wall of the first special-shaped water tank 200 satisfies 5230; and the second special-shaped water tank 400 is symmetrically arranged. The design of the included angles between the inclined walls and the inner walls of the special-shaped water tanks can reduce the area of water, and can also create a variety of flow velocity zones.

[0033] The principle that the device generates a complex flow field is as follow: as illustrated in FIG. 3, the water tank is divided into eight zones, with four zones on the left and other four zones on the right, which are symmetrical in pairs, wherein A and A are backflow zones; B and B are experimental zones; and C, C, D and D are slow flow zones. Because of the contact between the special-shaped water tank and the arc-shaped water tank, some backflows are produced in zones A and A, the flow velocity is small and these zones may be used as fish rest zones; C and C are the inlet zones of the flow pushers, the area is large, the flow velocity is also relatively small, and these zones may also be used as fish rest zones; B and B are in the arc-shaped water tanks, there is no obstruction and the flow velocity reaches the maximum; and due to flow division, the flow velocity in D and D is slower than that in B and B.

[0034] Experiments on spawning of parent fish will be performed based on the annular water tank:

[0035] 1. The parameters of the annular water tank were configured as follows: The outer diameter of the arc-shaped water tanks was 18 m, the length of the inner walls of the special-shaped water tanks was 15 m, the outer perimeter of the water tank was 87.33 m, the inner perimeter was 81.90 m, the width was 0.74 m and the depth was 1.70 m. For the actual situation, see FIG. 4.

[0036] 2. Preparation for experimental fish: Experimental fish were mature grass carp parent fish, as illustrated in FIG. 5, they were purchased in two batches, during the on-site acceptance period, they were identified by professionals, the gonad development period of the purchased parent fish was at stage IV or above, and they had spawning conditions. In the first batch, totally 23 parent fish (15 females and 8 males) were purchased, wherein 4 female fish escaped from the net during the temporary cultivation period, and the number of the actual experimental fish was 19 (11 females and 8 males); and in the second batch, totally 20 parent fish (10 females and 10 males) were purchased. In order to eliminate the influence of the transport process on fish, the experimental fish were domesticated in an artificial lake before the experiment started. The quality of water in the lake was excellent and the dissolved oxygen was sufficient, which could ensure the basic living environment of the fish.

[0037] 3. Implementation Steps of Experiments

[0038] (1) A water pump was started to inject tap water into the annular water tank. When the water level in the water tank reached the experimental requirement, the pump was stopped to stop injecting water. The tap water in the water tank was kept still for 3 days to remove harmful substance, i.e. chlorine in the water. Water temperature and dissolved oxygen concentration were recorded every 3 hours in the period of being still, and impurities falling into the water tank were taken out of the water.

[0039] (2) The parent fish samples were transferred into the annular water tank after the experimental water was kept still for 3 days. In order to eliminate the influence of the transfer process on the fish, the fish were put into the water tank for 1 day before the experiment.

[0040] (3) Each group of experiments was started from the flow velocity set under the working condition 1, the flow velocity was accelerated to the working condition 2 after 1 day, and so on until the working condition 4. Water quality data were recorded every 2 hours during the experiment. When the fish were exhausted during the experiment, the water flow pushing equipment was stopped as soon as possible, and the experiment continued after the fish renewed their physical strength. During the experiment, spawns were collected every 6 hours, the duration of spawn collection was 10 minutes, and the number of spawns collected at each time was recorded. There were 30 flowmeters which were uniformly distributed in the annular water tank and used to measure the flow velocity under each working condition. The set working conditions were specifically as follows:

TABLE-US-00002 TABLE 1 Working Maximum flow condition velocity Combination way of water flow pushing equipment 1 1.0 m/s First submersible pump + third submersible pump or second submersible pump + fourth submersible pump 2 1.2 m/s First submersible pump + third submersible pump + first flow pusher + second flow pusher or second submersible pump + fourth submersible pump + first flow pusher + second flow pusher 3 1.4 m/s First submersible pump + second submersible pump + third submersible pump + fourth submersible pump 4 1.6 m/s First submersible pump + second submersible pump + third submersible pump + fourth submersible pump + first flow pusher + second flow pusher

[0041] (4) Affected by the temperature condition and the fatigue state of the fish at the later stage of the experiment, experiments were additionally performed aiming at the working conditions 3 and 4, the experiment time was adjusted to nighttime, the time for each working condition was 3 hours and the interval between the working conditions was 3 hours.

[0042] (5) The spawning situations of the parent fish under the stimulation of the maximum flow velocity under different working conditions were obtained.

[0043] 4. Experimental Results

[0044] Flow velocity test results: After calculation of the actually measured flow velocity, the distribution in each zone is shown in Table 2.

TABLE-US-00003 TABLE 2 Maximum Working flow velocity Zone flow velocity (m/s) condition (m/s) A and A B and B C and C D and D 1 1.0 0.262 0.983 0.590 0.580 2 1.20 0.497 1.227 0.731 0.344 3 1.40 0.488 1.417 0.856 0.591 4 1.60 0.731 1.603 1.100 0.300

[0045] During the two groups of experiments, it was observed that the spawning behavior of the parent fish occurred at flow velocity of 1.4 m/s and 1.6 m/s, and both spawns and egg membranes were collected, but the number and scale of spawns were different. In the first group of experiments, the number of egg membranes was 6 at 1.4 m/s, the number of egg membranes was 183 at 1.60 m/s, and it is speculated that the number of spawning parent fish was at least 1, which spawned at 1.60 m/s, accounting for 9% of the total sample size of female parent fish; and no spawning behavior was found under the stimulation of other flow velocity. In the second group of experiments, different spawning situations were observed under the stimulation of flow velocity of 1.4 m/s and 1.6 m/s, wherein the spawning situation was found after 2 hours of continuous stimulation of flow velocity of 1.4 m/s, totally 50 egg membranes were collected, and after field check, it was found that at least one female parent fish had the spawning behavior, accounting for 10% of all female fish samples; and under the stimulation of flow velocity of 1.6 m/s, a large-scale spawning situation occurred, 600 spawns were collected within 10 minutes, and it is speculated that the number of spawning parent fish is five, accounting for 40% of all female fish samples. The proportion of spawning parent fish under the stimulation of different flow velocity is illustrated in FIG. 6 and FIG. 7. The water temperature and maturity conditions of the parent fish in the first group of experiments were different from that in the second group of experiments, which resulted in different data results. However, it can still be concluded from the results that the spawning of the parent fish is closely related to the flow velocity, and that the spawning of the parent fish could only occur under the stimulation of specific water flow velocity.