Pesticide composition containing thidiazuron and polyglutamic acid

Abstract

A pesticide composition, preparation method and use thereof; the composition having a synergism effect, including effective components A and B, wherein the active component A is thidiazuron, the active component B is polyglutamic acid, and the weight ratio of the component A to the component B is 1:(0.01-200).

Claims

1. A pesticide composition having a synergism effect, comprising two active components A and B, wherein the active component A is thidiazuron and the active component B is polyglutamic acid, and the weight ratio of the active component A to the active component B is 1:(0.01-200).

2. The composition according to claim 1, wherein the weight ratio of the active component A to the active component B is 1:(0.011-180).

3. The composition according to claim 2, wherein the weight ratio of the active component A to the active component B is (1:0.0125-180).

4. The composition according to claim 1, wherein the composition is prepared into a dosage form permissible in pesticide from the active components and a pesticide adjuvant.

5. The composition according to claim 4, wherein the dosage form is a dispersible oil suspending agent, wettable powder, water dispersible granules or an aqueous solution.

6. The composition according to claim 4, wherein the pesticide adjuvant is selected from one or more of a carrier, a solvent, a dispersant, a wetting agent, a tackifier, a thickener, an adhesive, a surfactant and a fertilizer.

7. A method comprising applying the pesticide composition according to claim 1 to plants or seeds that will produce the plants so as to promote or regulate growth of the plants.

8. A method comprising applying the pesticide composition according to claim 2 to plants or seeds that will produce the plants so as to promote or regulate growth of the plants.

9. A method comprising applying the pesticide composition according to claim 3 to plants or seeds that will produce the plants so as to promote or regulate growth of the plants.

10. A method comprising applying the pesticide composition according to claim 4 to plants or seeds that will produce the plants so as to promote or regulate growth of the plants.

11. A method comprising applying the pesticide composition according to claim 5 to plants or seeds that will produce the plants so as to promote or regulate growth of the plants.

12. The composition according to claim 1, wherein the polyglutamic acid is γ-polyglutamic acid.

Description

DETAILED DESCRIPTION

(1) In order to make the purposes, technical schemes and advantages of the present invention clearer, the present invention is further described in detail below in combination with examples. It should be understood that the specific examples described herein are only used for explaining the present invention, instead of limiting the present invention. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be regarded as within the protection scope of the present invention.

(2) The percent in all formulae of the examples below is weight percent (converted into percent). All technological processes of various preparations of the composition of the present invention are prior art, and can be changed somewhat according to different conditions.

(3) I. Examples for Preparation of Dosage Forms

(4) (I) Processing and Examples of Dispersible Oil Suspending Agent

(5) Components such as a dispersant, a wetting agent, a thickener and water were uniformly mixed according to a proportion of a formula; first, an emulsifier, the dispersant, the wetting agent, a stabilizer and a dispersion medium were added into a reaction kettle, and mixed and dispersed uniformly; and an antifreezing agent and the thickener were added into the reaction kettle in a high speed shearing state, and active components polyglutamic acid and thidiazuron were sanded in the presence of a zirconium bead grinding medium to prepare the dispersible oil suspending agent containing polyglutamic acid and thidiazuron.

Example 1: 2.025% Thidiazuron⋅Polyglutamic Acid Dispersible Oil Suspending Agent

(6) 2% of thidiazuron, 0.025% of polyglutamic acid, 6% of a diphenyl phenol polyoxyethylenepolyformaldehyde condensation compound, 2% of isopropanol, 1% of ammonium sulfate, 15% of alkylbenzenenaphthalenesulfonate and the balance methyl oleate, totaling 100%.

Example 2: 6.6% Thidiazuron⋅Polyglutamic Acid Dispersible Oil Suspending Agent

(7) 6% of thidiazuron, 0.6% of polyglutamic acid, 5% of castor oil polyoxyethylene ether, 3% of alkylphenolpolyoxyethyleneether phosphate, 15% of a polyoxyethylenepolyoxypropylene block copolymer, 4% of calcium dodecylbenzenesulfonate, 10% of attapulgite, 5% of organic bentonite, 5% of 2,6-di-tert-butyl p-cresol and the balance maize oil, totaling 100%.

Example 3: 10.2% Thidiazuron⋅Polyglutamic Acid Dispersible Oil Suspending Agent

(8) 0.2% of thidiazuron, 10% of polyglutamic acid, 15% of a polyoxyethylenepolyoxypropylene block copolymer (EO-PO block polymer), 3% of calcium dodecylbenzenesulfonate, 3% of castor oil polyoxyethylene ether, 2% of polyvinyl alcohol, 4% of aluminum-magnesium silicate, 2% of 2,6-di-tert-butyl p-cresol and the balance mineral oil, totaling 100%.

Example 4: 20.25% Thidiazuron⋅Polyglutamic Acid Dispersible Oil Suspending Agent

(9) 0.25% of thidiazuron, 20% of polyglutamic acid, 3% of alkylphenolpolyoxyethylene ether phosphate, 5% of a polyoxyethylenepolyoxypropylene block copolymer (EO-PO block copolymer), 3% of calcium dodecylbenzenesulfonate, 2% of white carbon black, 4% of organic bentonite, 4% of 2,6-di-tert-butyl p-cresol, 2% of glycerinum and the balance castor oil, totaling 100%.

(10) (II) Processing and Examples of Water Dispersible Granules

(11) The active components A and B were mixed with adjuvants and fillers uniformly according to proportions of the formulae; the mixture was crushed by an air flow to form wettable powder; then a certain amount of water was added to mix, extrude and prill; and drying and screening were conducted to prepare a water dispersible granule product.

Example 5: 3.06% Thidiazuron⋅Polyglutamic Acid Water Dispersible Granules

(12) 3% of thidiazuron, 0.06% of polyglutamic acid, 5% of lauryl sodium sulfate, 3% of white carbon black, 3% of alkyl sulfonate, 2% of bentonite, 3% of 2-bromo-2-nitryl-propyl-1,3-diol and the balance kaolin, totaling 100%.

Example 6: 24% Thidiazuron⋅Polyglutamic Acid Water Dispersible Granules

(13) 12% of thidiazuron, 12% of polyglutamic acid, 6% of ammonium sulfate, 5% of diatomite and the balance bentonite, totaling 100%.

Example 7: 13% Thidiazuron⋅Polyglutamic Acid Water Dispersible Granules

(14) 0.5% of thidiazuron, 12.5% of polyglutamic acid, 4% of sodium alkylbenzenesulfonate, 4% of lauryl calcium sulfate, 5% of white carbon black, 5% of calcium bicarbonate and the balance light calcium carbonate, totaling 100%.

(15) (III) Processing and Examples of Wettable Powder

(16) The active components thidiazuron and polyglutamic acid were fully and proportionally mixed with various adjuvants and fillers and the like, and the mixture was crushed by an ultrafine pulverizer to obtain the wettable powder.

Example 8: 1.01% Thidiazuron⋅Polyglutamic Acid Wettable Powder

(17) 1% of thidiazuron, 0.01% of polyglutamic acid, 5% of lignosulfonate, 6% of nekal, 4% of bentonite and the balance white carbon black, totaling 100%.

Example 9: 5.2% Thidiazuron⋅Polyglutamic Acid Wettable Powder

(18) 5% of thidiazuron, 0.2% of polyglutamic acid, 4% of nekal, 2% of Chinese honey locust powder, 3% of lauryl sodium sulfate, 2% of a molecular sieve and the balance kaolin, totaling 100%.

Example 10: 11% Thidiazuron⋅Polyglutamic Acid Wettable Powder

(19) 1% of thidiazuron, 10% of polyglutamic acid, 6% of sodium lignosulfonate, 4% of calcium dodecyl sulfonate, 2% of diatomite and the balance white carbon black, totaling 100%.

Example 11: 25.25% Thidiazuron⋅Polyglutamic Acid Wettable Powder

(20) 0.25% of thidiazuron, 25% of polyglutamic acid, 4% of aluminum-magnesium silicate, 3% of sodium dodecyl sulfate, 7% of sodium alkylbenzenesulfonate, % of bentonite and the balance light sodium carbonate, totaling 100%.

(21) (IV) Processing and Examples of Aqueous Solution

(22) The active components A and B were mixed with adjuvants and fillers uniformly according to proportions of the formulae, and the mixture and water were stirred and mixed to obtain an aqueous solution product.

Example 12: 12.1% Thidiazuron⋅Polyglutamic Acid Aqueous Solution

(23) 0.1% of thidiazuron, 12% of polyglutamic acid, 3% of alkylarylpolyoxyethyleneether phosphate, 2% of organic silicon, 8% of urea, 4% of sodium dodecylbenzenesulfonate, 2.5% of propylene glycol and the balance water, totaling 100%.

Example 13: 14.1% Thidiazuron⋅Polyglutamic Acid Aqueous Solution

(24) 0.1% of thidiazuron, 14% of polyglutamic acid, 9% of dodecylbenzene sulfonic acid, 2% of sodium acetate, 15% of kaolin, 3% of ammonium hydroxide and the balance water, totaling 100%.

Example 14: 16.1% Thidiazuron⋅Polyglutamic Acid Aqueous Solution

(25) 0.1% of thidiazuron, 16% of polyglutamic acid, 3% of monostearate, 6% of polyalkyl silicon ethylene oxide, 4% of nonylphenolpolyoxyethylene ether, 3% of castor oil polyoxyethylene ether and the balance water, totaling 100%.

Example 15: 18.1% Thidiazuron⋅Polyglutamic Acid Aqueous Solution

(26) 0.1% of thidiazuron, 18% of polyglutamic acid, 5% of polyalkyl silicon ethylene oxide, 2% of glycerol, 1% of hydroxy propyl cellulose, 2% of sodium benzoate, 1.5% of a polyisosuccinic anhydride-polyethylene glycol copolymer and the balance water, totaling 100%.

Example 16: 20.1% Thidiazuron⋅Polyglutamic Acid Aqueous Solution

(27) 0.1% of thidiazuron, 20% of polyglutamic acid, 2% of dimethyl phthalate, 5% of allyl carbonate, 0.5% of N-n-octylpyrrolidone, 4% of monopalmitate, 3% of polyethylene glycol, 2% of hydroxyethyl cellulose and the balance water, totaling 100%.

Example 17: 22.1% Thidiazuron⋅Polyglutamic Acid Aqueous Solution

(28) 0.1% of thidiazuron, 22% of polyglutamic acid, 1% of hydroxy propyl cellulose, 4% of nonylphenolpolyoxyethylene ether, 3% of isopropylene glycol, 1.8% of a polyisosuccinic anhydride-polyethylene glycol copolymer and the balance water, totaling 100%.

(29) II. Pesticide Effect Verification Test

(30) (I) Bioassay Examples

(31) 1. Comprehensive Assay Test of Compounding Thidiazuron and Polyglutamic Acid on Growth Regulation of Indica

(32) Thidiazuron and polyglutamic acid were diluted with acetone according to a certain concentration and were sprayed to stems and leaves of crops, and influence of concentrations on growth of crops is assayed in the field, including comprehensive evaluation on plant height, spike number, setting percentage, disease-resistant condition, yield condition and the like of the crops.
Plant height control rate=(plant height of a clean water control group-processed plant height)/plant height of the clean water control group*100%
Spike number increase ratio=(processed plant spike number-plant spike number of a clean water control group)/plant spike number of the clean water control group*100%
Setting percentage=filled grain number per spike/total grain number per spike*100%
Prevention effect=(disease index of a clean water control group-processed disease index)/disease index of the clean water control group*100%
Yield per mu increase ratio=(processed yield per mu-yield per mu of a clean water control group)/yield per mu of the clean water control group*100%

(33) TABLE-US-00001 TABLE 1 Summery of comprehensive assay of compounding thidiazuron and polyglutamic acid on growth regulation of indica Spike Setting Disease Number Percentage Control Spike Total filled Disease Plant Number Grain grain Index Production Height of Number number Setting of Prev- Increase Ratio Dosage Plant Control Single Increase of per Percen- Banded ention Output Increase Name (a.i.mg/ Height Rate Plant Ratio Spike spike tage Sclerotial Effect (667 Ratio of Pesticide kg) (cm) (%) (grains) (%) (grains) (grains) (%) Blight (%) M.sup.2) (%) Thi:Poly = 50 116.0 9.1 18.8 8.0 144.2 132.8 92.1 7.9 73.2 555.5 9.0 1:0.008 Thi:Poly = 50 114.5 10.2 19.1 9.8 148.2 138.6 93.5 6.8 76.9 565.5 11.0 1:0.01 Thi:Poly = 50 112.3 12.0 19.3 10.9 149.0 140.7 94.4 6.6 77.5 571.5 12.1 1:0.0125 Thi:Poly = 50 111.4 12.7 19.5 12.1 151.7 144.9 95.5 6.5 78.1 577.5 13.3 1:0.02 Thi:Poly = 50 110.1 13.8 19.7 13.2 153.6 148.1 96.4 6.2 78.9 584.0 14.6 1:0.04 Thi:Poly = 50 108.4 15.0 19.9 14.4 149.2 144.9 97.1 6.0 79.6 588.0 15.4 1:0.1 Thi:Poly = 50 107.6 15.7 20.1 15.5 154.8 151.4 97.8 5.7 80.6 593.5 16.5 1:1 Thi:Poly = 50 106.5 16.6 20.2 16.1 155.2 151.3 97.5 5.3 82.2 597.0 17.1 1:10 Thi:Poly = 50 107.6 15.7 19.8 13.8 153.6 148.5 96.7 5.4 81.6 593.0 16.4 1:25 Thi:Poly = 50 109.2 14.4 19.7 13.2 148.9 142.8 95.9 5.9 79.9 590.5 15.9 1:50 Thi:Poly = 50 110.1 13.8 19.5 12.1 152.7 145.2 95.1 6.1 79.4 587.0 15.2 1:80 Thi:Poly = 50 110.4 13.5 19.5 12.1 150.1 142.3 94.8 6.3 78.7 585.0 14.8 1:100 Thi:Poly = 50 111.3 12.8 19.3 10.9 149.8 141.3 94.3 6.4 78.4 583.5 14.5 1:120 Thi:Poly = 50 112.0 12.2 19.2 10.3 149.1 140.3 94.1 6.5 77.8 580.5 13.9 1:140 Thi:Poly = 50 113.1 11.3 19.1 9.8 148.7 138.9 93.4 6.8 77.0 577.0 13.2 1:160 Thi:Poly = 50 114.3 10.5 19.1 9.8 147.2 136.9 93.0 6.9 76.5 570.0 11.9 1:180 Thi:Poly = 50 115.1 9.8 19.0 9.2 146.8 135.4 92.2 7.1 75.8 568.0 11.5 1:200 Thi:Poly = 50 116.6 8.6 18.8 8.0 146.1 134.0 91.7 8.2 72.1 559.5 9.8 1:220 Thidiazuron 50 121.2 5.0 18.1 4.0 145.7 132.2 90.7 14.8 49.8 537.5 5.5 Polyglutainic 50 121.9 4.4 18.2 4.6 143.3 131.2 91.6 21.1 28.6 535.5 5.1 acid Clean water — 127.6 — 17.4 — 128.7 109.8 85.3 29.5 — 509.6 — control group (CK) Note: Thidiazuron is abbreviated as “Thi” and polyglutamic acid is abbreviated as “Poly” in the table. Pesticide application stage: blooming stage, in which the indica plant had not been or had been suffered from banded sclerotial blight. Pesticide application number of times: 2 times, 8 days as a pesticide application interval. Pesticide application mode: stem and leaf spraying. Investigation stage: later indica grouting stage. In the test, indexes such as plant height, spike number, disease index and setting number are processed mean values.

(34) It is found by the inventors through experiments that thidiazuron and polyglutamic acid play a good synergistic promoting role. The compounded thidiazuron and polyglutamic acid in different proportions are obvious in regulating growth of indica compared with individual thidiazuron and polyglutamic acid as well as the clean water control group. The compounded thidiazuron and polyglutamic acid has the main advantages that 1, thidiazuron as the plant growth regulator can promote plant cell division and plant growth, and in the process, rice needs to absorb more nutrients (such as fertilizers, amino acids, humic acid and other nutritional organic matters) from outside to meet the plant growth, and polyglutamic acid as a metabolite of beneficial bacteria can serve as a fertilizer to provide nutrients needed by thidiazuron in promoting growth of rice. Nutrient conduction and disease resistance and stress resistance of the rice plants are promoted by means of synergism effect of thidiazuron and polyglutamic acid. In the mature period of rice, thidiazuron and polyglutamic acid have an obvious enhancing effect to unit yield per mu, and the yield per mu increase ratio is up to 17.1%. 2, it is found in the test that polyglutamic acid itself also has a function of regulating plant growth and plays a complementary role with thidiazuron, so that the plant height of rice can be reduced effectively and the up-down nutrient conduction ability of the plant can be improved effectively. Furthermore, lodging resistance in rice growth can be improved by reducing the plant height. 3, thidiazuron used as a single dosage has certain disease resistance and stress resistance to plant growth while polyglutamic acid has certain immunity to crop diseases. Joint application of polyglutamic acid and thidiazuron has a good synergism effect. It can be seen from analysis in the above table that compared with the single dosage and the clean water control group, polyglutamic acid and thidiazuron have a good prevention effect to banded sclerotial blight of indica due to synergism effect between polyglutamic acid and thidiazuron.

(35) (II) Field Pesticide Effect Verification Test

(36) 1. Field Comprehensive Test of Mixing Thidiazuron and Polyglutamic Acid on Growth Regulation and Disease Resistance and Production Increase of Maize Crops

(37) Sorghum variety: Suyu 23.

(38) Pesticide application stage: a big horn stage of maize and a blooming stage of maize in which diseases such as Southern corn leaf blight are prone to occur.

(39) Pesticide application number of times: 2 times, 6 days asan interval. The examples of the preparation of the present invention are sprayed for three repeated times, and the result is the mean value.

(40) TABLE-US-00002 TABLE 2 Field comprehensive test of mixing thidiazuron and polyglutamic acid on growth regulation of maize Stem Southern Preven- Thousand Dosage Plant Control Dia- Increase corn tion seed Increase Increase Name (a.i. Height Rate meter Ratio leaf Effect weight Ratio Output Ratio of Pesticide mg/kg) (cm) (%) (cm) (%) blight (%) (g) (%) (667M.sup.2) (%) Example 1: 2.025% 40 120.2 11.8 2.66 12.2 7.32 75.73 264.8 12.1 687.6 14.4 thidiazuron .Math. polyglutamic acid dispersible oil suspending agent (80:1) Example 2: 6.6% 40 116.2 14.7 2.77 16.8 6.32 79.04 271.2 14.8 705.0 17.3 thidiazuron .Math. polyglutamic acid dispersible oil suspending agent (10:1) Example 3: 10.2% 40 116.3 14.6 2.74 15.5 7.02 76.71 269.8 14.2 691.9 15.1 thidiazuron .Math. polyglutamic acid dispersible oil suspending agent (1:50) Example 4: 20.25% 40 116.9 14.2 2.72 14.6 7.23 76.02 267.1 13.1 686.9 14.3 thidiazuron .Math. polyglutamic acid dispersible suspending agent (1:80) Example 5: 3.06% 40 119.0 12.7 2.69 13.4 6.65 77.94 266.8 13.0 691.3 15.0 thidiazuron .Math. polyglutamic acid water dispersible granules (50:1) Example 6: 24% 40 114.7 15.9 2.81 18.7 6.09 79.81 273.5 15.8 710.0 18.1 thidiazuron.Math. polyglutamic acid water dispersible granules (1:1) Example 7: 13% 40 115.8 15.0 2.78 17.3 6.29 79.15 270.3 14.4 700.6 16.6 thidiazuron .Math. polyglutamic acid water dispersible granules (1:25) Example 8: 1.01% 40 121.6 10.8 2.63 11.1 7.60 74.80 262.0 10.9 677.6 12.7 thidiazuron .Math. polyglutamic acid wettable powder (100:1) Example 9: 5.2% 40 117.9 13.5 2.73 15.1 6.67 77.87 268.2 13.6 699.4 16.4 thidiazuron .Math. polyglutamic acid wettable powder (25:1) Example 10: 11% 40 113.7 16.6 2.84 19.7 5.89 80.45 274.2 16.1 711.2 18.3 thidiazuron .Math. polyglutamic acid wettable powder (1:10) Example 11: 25.25% 40 117.7 13.6 2.71 14.2 7.55 74.94 266.4 12.8 683.2 13.7 thidiazuron .Math. polyglutamic acid wettable powder (1:100) Example 12: 12.1% 40 118.7 12.9 2.70 13.8 7.73 74.35 265.2 12.3 682.0 13.5 thidiazuron .Math. polyglutamic acid aqueous solution (1:120) Example 13: 14.1% 40 120.3 11.7 2.69 13.5 7.82 74.07 263.4 11.5 680.7 13.2 thidiazuron .Math. polyglutamic acid aqueous solution (1:140) Example 14: 16.1% 40 121.8 10.6 2.66 12.3 8.12 73.06 261.7 10.8 678.2 12.8 thidiazuron .Math. polyglutamic acid aqueous solution (1:160) Example 15: 18.1% 40 122.1 10.4 2.65 11.9 8.31 72.44 260.8 10.4 675.7 12.4 thidiazuron .Math. polyglutamic acid aqueous solution (1:180) Example 16: 20.1% 40 122.7 10.0 2.64 11.5 8.93 70.40 260.3 10.2 674.5 12.2 thidiazuron .Math. polyglutamic acid aqueous solution (1:200) Example 17: 22.1% 40 124.1 9.0 2.61 10.1 9.62 68.10 257.6 9.1 663.5 10.4 thidiazuron .Math. polyglutamic acid aqueous solution (1:220) thidiazuron 40 129.0 5.4 2.50 5.4 14.74 51.11 249.0 5.4 637.9 6.1 polyglutamic acid 40 131.5 3.5 2.48 4.5 25.45 15.59 246.0 4.1 633.7 5.4 Clean water — 136.3 — 2.37 — 30.15 — 236.2 — 601.1 — control group (CK)

(41) It can be seen from experimental data in the table 2 that thidiazuron and polyglutamic acid have an obvious synergism effect, specifically: 1, maize plants are promoted to grow regularly, weak seedlings are promoted to be strong, big seedlings become dwarf, leaves are wide, thick and strongly green, chlorophyll is increased, the functional stage of the leaves is prolonged, the photosynthesis is enhanced, vigorous growing control and excessive growth of maize are controlled effectively, the stem diameters of maize are increased, nutrient conduction is promoted, and lodging of maize is prevented; 2, disease resistance and stress resistance of the maize plants are induced, so that the health benefit of the plants is improved; and 3, full seeds of maize are promoted, the thousand seed weight of maize seeds is improved, the per unit output of maize is improved obviously, and the unit yield per mu increase ratio is up to 18.3%.