Device and method of microbiology waste containment

Abstract

A microbiology waste containment apparatus and method are provided to prevent toxic slide staining chemicals from being dumped down a drain during slide staining and thus entering publicly owned water treatment works. A primary containment vessel is provided having side walls and an open top area, and a funnel is provided in communication with the open top area. A secondary containment vessel is in fluid communication with the funnel. When microbiology waste is generated above the open top area, the microbiology waste is collected by the funnel and conducted to the secondary containment vessel. The secondary vessel may be disposed internally to the primary vessel, or it may be external to the primary vessel. In the case of the internal secondary vessel, the entire apparatus may be portable and thus appropriate for field or military work.

Claims

1. A portable method of containing microbiology waste generated during a microbiology slide staining procedure, comprising the steps of: i) providing a portable carriable primary containment vessel having side walls and an open top area; ii) placing a funnel in the open top area of the primary containment vessel, the funnel dimensioned to fit within and rest atop the open top area; iii) providing a secondary containment vessel in fluid communication with the funnel; iv) performing a microbiology slide staining procedure above the open top area, the microbiology slide staining procedure generating microbiology waste; v) collecting the microbiology waste generated by said performing step iv) by the funnel; and vi) conducting the waste collected by the funnel in said collecting step v) to the secondary containment vessel.

2. A method of containing microbiology waste generated during a microbiology slide staining procedure according to claim 1, wherein the secondary containment vessel is an inner containment vessel and the method further comprises the step of disposing the inner containment vessel within the primary containment vessel.

3. A method of containing microbiology waste generated during a microbiology slide staining procedure according to claim 1, wherein the secondary containment vessel is an external containment vessel and the method further comprises the step of disposing the external containment vessel outside of the primary containment vessel.

4. A method of containing microbiology waste generated during a microbiology slide staining procedure according to claim 1, further comprising the step of providing a slide staining support disposed in one of a) the open top area, or b) the funnel, adapted to support slides being prepared with chemicals that become microbiology waste following the slide staining procedure.

5. A method of containing microbiology waste generated during a microbiology slide staining procedure according to claim 1, the funnel being dimensioned substantially the same size as the open top area.

6. A method of containing microbiology waste generated during a microbiology slide staining procedure according to claim 1, wherein the secondary containment vessel includes an inner containment vessel disposable within the primary containment vessel.

7. A method of containing microbiology waste generated during a microbiology slide staining procedure according to claim 1, further comprising the steps of: providing a lid lockable onto the primary containment vessel; and providing at least one handle disposed on an exterior of at least one of a) the lid, or b) the primary containment vessel, wherein the primary containment vessel is carriable and portable.

8. A method of containing microbiology waste generated during a microbiology slide staining procedure according to claim 1, wherein the funnel comprises flanges adapted to engage a lip in the primary containment vessel just below the open top area.

9. A method of containing microbiology waste generated during a microbiology slide staining procedure according to claim 8, further comprising the step of adjusting the lengths of the flanges to accommodate primary containment vessels of different sizes.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) FIGS. 1A-B: Lateral and dorsal view of a funnel, showing positions of drains, dowells, and brackets, in accordance with the invention.

(2) FIG. 2: Dorsal and lateral view of alternative rounded edge funnel brackets, in accordance with the invention.

(3) FIG. 3: Dorsal and lateral view of alternative square edge funnel brackets, in accordance with the invention.

(4) FIGS. 4A-B: Lateral and dorsal views, respectively, of inner waste containment vessel, in accordance with the invention.

(5) FIG. 5: Lateral view of outer containment vessel, in accordance with the invention.

(6) FIG. 6: Side sectional view of funnel and inner containment vessel connected to each other and disposed within the outer containment vessel, in accordance with the invention.

(7) FIG. 7: Side sectional view of funnel disposed within the outer containment vessel and connected to larger external containment vessel, in accordance with the invention.

(8) FIG. 8: Schematic depicting dorsal and side signage for the device, especially the outer containment vessel.

(9) FIGS. 9 and 10: Showing relative position of stain solutions located in the outer containment vessel, either next to the interior waste containment vessel (FIG. 9) or in the containment vessel when the outer (extrinsic) waste container is in use (FIG. 10).

(10) FIGS. 11 A-B: Lateral and dorsal views, respectively, of lid_which covers the vessel and can function as containment for the microscope slides prior to being stained or as a staging area for processed microscope slides.

(11) FIGS. 12A-B is a schematic of a fully self-contained and portable microbiology staining and waste containment kit in accordance with the invention. FIG. 12A shows the kit with the lid open, and FIG. 12B shows the kit with the lid closed.

DETAILED DESCRIPTION OF THE INVENTION AND DRAWINGS

(12) Description will now be given with reference to the attached FIGS. 1-12. It should be understood that these figures are exemplary in nature and in no way serve to limit the scope of the invention, which is defined by the claims appearing hereinbelow. All dimensions, materials, and the like are also exemplary in nature and can be replaced with other dimensions, materials, and the like without departing from the scope of the invention where practical and advantageous.

Components

(13) Primary outer containment vessel 10 (see FIG. 5). A plastic box product preferably, in one embodiment, of the dimensions 18 long (outside), 12 wide (outside), 13 Outer height and 12 inner height with a lip around the top 1 distally, and the 1 up creating a rim 11. One or more sealable exit ports 12 (one shown in FIG. 7) are provided for plastic tubing in the center (6.5) of the width side, and 6 from the bottom, one on either side. The exit ports are to accommodate tubing, e.g., 5/16 ID tubing, which may be fed to a larger (e.g., 5 Gallon) external labeled waste vessel (see FIG. 7). The port gives laboratories that generate larger volume the option of conveniently capturing that volume. The tube, space permitting, could be fed to an even larger container, as allowable by local and federal authorities. For example, at the time of this writing, EPA regulations allow a maximum of 55 gallon of waste in a Satellite Accumulation Area for a period of no more than one year. Many states, such as New York, are more stringent and only allow accumulation for 180 days. The City of New York Fire Code allows no more than a total of 30 Gallons of flammable liquids per laboratory space, including both product and waste.

(14) The interior 14 of the outer containment vessel 10 has sufficient room for the stain solutions 6, 7, and 8 (see FIGS. 9 and 10), which should be labeled on the top of the bottle as to contents by the user. The interior space also allows the manipulation of the drain and fill valves that leave and enter the two interior components.

(15) Each Length side of the outer containment vessel will preferably be labeled with appropriate warnings, such as shown in FIG. 8 and including optionally the following:

(16) Microbiology Containment and Satellite Accumulation Area.

(17) Please keep closed when not in use.

(18) Full waste containers must be brought to the Central (Main) Accumulation Area within 72 hours.

(19) No waste may be stored for more than ______ days (as per state and/or federal regulations).

(20) Label stating contents (Methanol Stain) and start date.

(21) Warning labels may be placed on other locations of outer containment vessel 10.

(22) Lid 20 (see FIGS. 11A-B): Lid 20 is made to fit with this modified commercially available product. This cover closes the entire unit and can function as containment when placed upside down on a flat surface. The inner components can be placed upon the upside down lid during maintenance operations. The top of the lid shall be labeled appropriately, e.g. as follows:

(23) Microbiology Containment and Satellite Accumulation Area

(24) Please keep closed when not in use.

(25) Full waste containers must be brought to the Central (Main) Accumulation Area within 72 hours.

(26) No waste may be stored for more than [X] days (as per state and/or federal regulations).

(27) Label stating contents (Methanol Stain) and start date.

Secondary Containment Vessel Option 1

(28) Inner Waste Accumulation Vessel 30 (see FIGS. 4A-B): This is to enable microbiology laboratories with small volume and or limited space to accumulate waste into a reusable container that can be decanted into a larger vessel in the Central (Main) Accumulation Area.

(29) In one embodiment, the dimensions of vessel 30 are: 17 Length, 7 width, and 4 height. The dorsal surface 32 preferably has a handle 34 located 11 from the filling end, a vent 35 about 2 on center from the vent end, and a fill port 36 with a valve cap 38 3 on center from the filling end. The filling end is connected to the funnel 40 via a piece of 5/16 inner diameter (ID) tubing 31 to the fill port 36. A separate solid cap (not shown) is supplied to seal the collection vessel so that another one can be put into place. Another valved cap (not shown) can be used to decant the waste into a larger container in the Central Accumulation Area. This container 30 shall be labelled as to contents with a date within regulatory limits. When this container 30 is full, container 30 (or the contents of container 30), are supposed to be removed to the Central Accumulation Area within 72 hours or the like.

(30) Microbiology Funnel 40 (see FIG. 1): The length of the exemplary funnel body is preferably 14 inches. In one embodiment, the width of the funnel body 6 and inches. On each width end is a 1-inch telescoping flange 42 to hold the funnel on/in the upper lip portion 11 of the outer containment vessel 10. It is to fit so that funnel 40 may be slid width-wise in the containment box 10 easily with minimal forward/backward motion. The attachment flanges 42 are adjustable to give the user options. In the preferred embodiment, flanges 42 are made from a stiff wire or coated wire-type structure. An alternative flange 42 with rounded edges is shown in FIG. 2, and an alternative flange 42 with square support surfaces is shown in FIG. 3. Flanges 42 and 42 are optionally interchangeable with flange 42 to give the user options of applications, such as using different containerization, among other things. As another alternative, funnel 40 is simply dimensioned to sit atop outer containment vessel 10 without adjustable flanges, e.g., either with fixed/permanent flanges or with no flanges per s.

(31) In one embodiment, the maximum depth of funnel 40 is preferably 2 inches at the fill end, centered on 4 inches lengthwise. From the point of 4 inches from the back funnel wall, the floor of the funnel ramps up to 1 inches at the other end, for a distance of Run 10 inches, a Hypotenuse 11 inches, with a drop of 1 inch from the higher end to the drain end. At each end, slightly recessed from the funnel lip ( inch), are brackets 44 located 1 inches width at the widest on center to hold two parallel dowels 46 for supporting microscope slides during staining procedures. Although the dowels may be round in section, preferably they have a flattened upper surface so as to provide greater surface area of support for the slides and deter slipping of the slides during staining. Alternatively, slide support dowels 46 may be attachable to outer containment vessel 10 above funnel 40. Additionally, the upper surface of dowels 46 may be provided with a high coefficient of friction (e.g., a non-slip and non-reactive material, or the same material but made rough, etc.) to deter slipping of the slides during staining even further. This may not be necessary as most slideracks are smooth and the weight of the glass is sufficient to keep the slide relatively immobile, even when they are being sprayed with staining material. In any case, dowels 46 are preferably removable for cleaning and replacement purposes. The top of the dowels is inches recessed from the top of the funnel. The dowels need be no more than inches in diameter.

(32) The funnel drain 48 is located 2 inches on center from the deep end. The drain port feeds into a ball valve tube 49 (see FIG. 6). The ball valve drain tube 49 is attached to plastic tubing 31 which feeds into either the inner waste containment vessel 30 or through one of the ports 12 (located on either narrow side) into a larger, contained, waste accumulation vessel.

(33) This device is preferably manufactured from injection molded chemically resistant polypropylene or a similar polymeric or otherwise non-reactive material.

(34) In operation, the invention is used as follows.

(35) Place the entire apparatus 5 on a flat surface.

(36) Remove the lid 20 from the primary containment vessel 10 and place upside down (open side up) on a flat surface. The upside down lid 20 may be utilized as containment for the working slides or any other material utilized in the staining process not contained in the outer containment vessel 10. Assemble the drain cap on the funnel and put the valve in the closed position, with the valve handle across the flow direction.

Option 1 (see FIG. 6)

(37) Using 5/16 ID tubing, connect the funnel valve to the fill valve on the inner waste collection vessel.

(38) Examine the connections to ensure a liquid tight fit.

(39) Open the vent 35 in the back end (near the handle 34) of the inner collection vessel 30.

(40) Open the drain valve 49 on the bottom of the funnel and the fill valve 38 on top of the inner waste containment vessel 30. Label the collection (inner containment) vessel 30 as to content (for example Methanol Stain) with a start date within 180 days (or 1 year, if applicable). Label the working stain solutions 6, 7, 8 (product) dorsally as to content, in plain and clear language, and place in the outer containment box 10 in storage area 13 between the inner containment vessel 30 and the near outer containment vessel wall.

(41) Begin staining and monitor the inner containment vessel 30 so as not to overfill. The inner containment vessel 30 is preferably manufactured out of semi-opaque white plastic. As this will soon be opaque from the waste stain solution, the level may be monitored by observing the level in the container through the vent port, at the opposite end of the fill port on the vessel. (Optionally, a clear section may be formed in inner containment vessel 30.) Take care not to overfill, however if overfilling occurs, it is not a big problem because the waste material is still contained by the outer containment vessel 10.

(42) When full, decant inner containment vessel 30 into a larger container in the Central Accumulation Area or seal and replace the container after placing the full container in the Central (Main) Accumulation area. Label the container as to contents and date the container utilizing a Hazardous Waste Accumulation label. (Exemplary contents: Methanol Stain Solutions.)

Option 2 (see FIG. 7)

(43) Using 5/16 inner diameter tubing 31, run the tubing through one of the side ports 12 in the outer containment vessel 10 to an external waste container and connect to the funnel drain valve 49. If so desired, put another valve (not shown) on the drain end entering the collection container. Label the external waste container as to content and date within 180 days or 1 year if applicable. Preferably provide spill control containment in or on the external waste container. If the external waste container is a five gallon plastic pail, the outer containment vessel should be large enough to contain 5 gallons of liquid.

(44) Examine the connections from the funnel valve 49 and at the external waste container to ensure a liquid tight fit.

(45) Open the valves.

(46) Begin staining and monitor the waste container(s) so as not to overfill.

(47) When full, move the labeled full container in the Central (Main) Accumulation Area and replace with an empty labeled container.

(48) When complete or leaving the room, please make sure waste collection vessels are closed. Please make sure the drain valves are in the closed (cross stream) position. Please place the lid on the device.

(49) The invention is not limited to the above description. For example, the funnel, with either flange attachment, may be used over a different waste collection container or outer containment vessel as the Waste Generator so chooses. The generator should be mindful of containment with whichever option is used. Also, the wholly contained system having the inner secondary containment vessel is aptly designed for portable use, e.g., either to be carried from one location in a lab to another or from one lab to another, or for use in the field, e.g., during a rural disease outbreak, during wartime, etc. In such cases, as shown in FIGS. 12A-B, the lid will preferably lock to the outer containment vessel 10 via locking mechanism 60, and the device will include one or more handles 62, either disposed on the exterior of the lid, or on the exterior of the outer containment vessel, or the like. In the embodiment of FIG. 12, lid 20 may be hingedly attached to outer vessel 10 via hinges 64 so as not to get separated. Alternatively, the lid could be completely removable from vessel 10 and still lock thereto, e.g., via mason-jar-type clips, or the like. In either event, funnel 40 may be stored upside-down atop inner containment vessel 30 as shown in FIG. 12B. Funnel flanges 42 may telescope out of the way as mentioned above, or they may be hinged to fold out of the way, or be otherwise detachable and storable within vessel 10.

(50) Additionally, the invention includes a non-transitory computer readable medium having instructions for making the above apparatus. The methods of making the apparatus can include additive manufacturing such as 3D printing as well as any known or to be developed methods of manufacturing.

(51) Having described certain embodiments of the invention, it should be understood that the invention is not limited to the above description or the attached exemplary drawings. Rather, the scope of the invention is defined by the claims appearing below and includes any equivalents thereof as would be appreciated by one of ordinary skill in the art.