Antimicrobial composition having efficacy against endospores

Abstract

A sporicidal composition has a moderately low pH and includes at least one oxidizing acid and the dissociation product of at least one inorganic oxidizing agent. Very high effective solute concentrations can enhance the efficacy of the composition. Embodiments of the composition can be applied to a surface and allowed to absorb into the endospore, ultimately killing at least some of those bacteria in mature endospore form. The surface being treated can be an inanimate surface, particularly a hard surface, or a medical device.

Claims

1. A sporicidal composition having a pH of from 1.5 to 4, inclusive, and an effective solute concentration of from 1.5 to 9 Osm/L, inclusive, said composition comprising, on a per liter basis: a) a solvent component having a .sub.p value less than 15.6 MPa.sup.1/2 that comprises 1) 50-500 mL water and 2) at least one organic liquid, and b) a solute component that comprises 1) dissociation product of an oxidizing acid having a pK.sub.a value of greater than 3 and a standard potential of at least +0.5 V, 2) dissociation product of from 4 to 20 g of an electrolyte oxidizing agent having a standard potential of at least +1.5 V, and 3) dissociation product of at least one non-oxidizing electrolyte.

2. The sporicidal composition of claim 1 wherein said solute component further comprises wetting agent.

3. The sporicidal composition of claim 2 wherein said oxidizing acid is the reaction product of an organic acid and a peroxide.

4. The sporicidal composition of claim 2 having a pH of no more than 3 and an effective solute concentration of at least 2 Osm/L.

5. The sporicidal composition of claim 2 wherein said wetting agent comprises anionic surfactant.

6. The sporicidal composition of claim 5 wherein said wetting agent further comprises nonionic surfactant.

7. The sporicidal composition of claim 1 wherein said oxidizing acid is the reaction product of an organic acid and a peroxide.

8. The sporicidal composition of claim 1 having a pH of no more than 3 and an effective solute concentration of at least 2 Osm/L.

9. The sporicidal composition of claim 1 wherein said at least one organic liquid comprises a glycol ether.

10. The sporicidal composition of claim 1 wherein said at least one organic liquid is a glycol ether.

11. The sporicidal composition of claim 1 having an effective solute concentration of at least 3 Osm/L.

12. The sporicidal composition of claim 1 wherein said solvent component has a .sub.p value of from 13.5 to 15.5 MPa.sup.1/2.

13. A method for treating a surface susceptible to the presence of one or more types of endospores, said method comprising contacting said surface with a sporicidal composition having a pH of from 1.5 to 4, inclusive, and an effective solute concentration of from 1.5 to 9 Osm/L, inclusive, said composition comprising, on a per liter basis: a) a solvent component having a .sub.p value less than 15.6 MPa.sup.1/2 that comprises 1) 50-500 mL water and 2) at least one organic liquid, and b) a solute component that comprises 1) dissociation product of an oxidizing acid having a pK.sub.a value of greater than 3 and a standard potential of at least +0.5 V, 2) dissociation product of from 4 to 20 g of an electrolyte oxidizing agent having a standard potential of at least +1.5 V, and 3) dissociation product of at least one non-oxidizing electrolyte.

14. A sporicidal composition having a pH of no more than 3 and an effective solute concentration of at least 2 Osm/L, said composition comprising, on a per liter basis: a) a solvent component having a .sub.p value of from 13.5 to 15.5 MPa.sup.1/2, that consists of 1) 50-500 mL water and 2) at least one organic liquid that comprises glycol ether, and b) a solute component that comprises 1) dissociation product of an oxidizing acid having a pK.sub.a value of greater than 3 and a standard potential of at least +0.5 V, said oxidizing acid being the reaction product of an organic acid and a peroxide, 2) dissociation product of from 4 to 20 g of an electrolyte oxidizing agent having a standard potential of at least +1.5 V, 3) dissociation product of at least one non-oxidizing electrolyte, and 4) a wetting agent that comprises anionic surfactant.

15. The sporicidal composition of claim 14 wherein said electrolyte oxidizing agent has a standard potential of at least +2.0 V.

16. The sporicidal composition of claim 15 wherein said wetting agent further comprises nonionic surfactant.

17. The sporicidal composition of claim 15 wherein said solute component comprises 12.56 g of said electrolyte oxidizing agent.

18. The sporicidal composition of claim 14 wherein said wetting agent further comprises nonionic surfactant.

19. The sporicidal composition of claim 18 wherein said solute component comprises 12.56 g of said electrolyte oxidizing agent.

20. The sporicidal composition of claim 14 wherein said solute component comprises 12.56 g of said electrolyte oxidizing agent.

Description

DETAILED DESCRIPTION OF ILLUSTRATIVE EMBODIMENTS

(1) The sporicidal composition is described first in terms of its properties and components, many of which are widely available and relatively inexpensive, and then in terms of certain uses.

(2) The solvent component of the composition typically includes a significant amount of water. Relative to its overall volume, a composition often includes up to 20%, 25%, 30%, 33%, 35%, 40%, 45%, 50%, or even 55% (all v/v). On a per liter basis, a composition often includes from 50 to 500 mL, commonly from 75 to 475 mL, more commonly from 100 to 450 mL, usually from 125 to 425 mL, typically from 150 to 400 mL, and most typically 25050 mL water. The water need not be specially treated (e.g., distilled and/or deionized), although preference certainly can be given to water that does not interfere with the intended antimicrobial effect of the composition.

(3) The solvent component of the composition often includes at least one organic liquid, and, in some embodiments, preference is given to those organic liquids with .sub.p values lower than that of water (.sub.p16.0 MPa.sup.1/2). Where at least one organic liquid is present in the solvent component, the .sub.p value of the overall solvent component generally is less than 16.0, no more than 15.6, no more than 15.2, no more than 15.0, no more than 14.6 or no more than 14.0 MPa.sup.1/2. In some embodiments, the .sub.p value of the overall solvent component can range from 13.1 to 15.7 MPa.sup.1/2, from 13.3 to 15.6 MPa.sup.1/2, from 13.5 to 15.5 MPa.sup.1/2, and even from 13.7 to 15.4 MPa.sup.1/2.

(4) The organic liquid(s) often is/are present at concentrations of up to 60%, commonly 5 to 50%, more commonly 10 to 45%, even more commonly 15 to 40%, and typically 20 to 35% (all w/v, based on total volume of solvent component).

(5) The amount of a given organic liquid (or mixture of organic liquids) to be added to water can be calculated using formula (I) if a targeted .sub.p value is known. Similarly, a projected .sub.p value can be calculated using formula (I) if the amount of organic liquid(s) and their individual .sub.p values are known.

(6) The solvent component can consist of, or consist essentially of, only water or only one or more organic liquids, with preference being given to mixtures of species of the same genus of organic liquids, e.g., two ethers or two alcohols rather than one ether and one alcohol. In certain preferred embodiments, the solvent component can consist of, or consist essentially of, water and an organic liquid, preferably one having a .sub.p value less than 15.5 MPa.sup.1/2. In yet other embodiments, the solvent component can consist of, or consist essentially of, water and two or more organic liquids, with the resulting solvent component having .sub.p value that can be calculated using formula (I); again, preference is given to mixtures of species of the same genus of organic liquids, e.g., two ethers or two alcohols rather than one ether and one alcohol.

(7) With respect to organic liquids that can be employed in the solvent component, those which are miscible with one another and/or water are preferred. Non-limiting examples of potentially useful organic liquids include ketones such as acetone, methyl butyl ketone, methyl ethyl ketone and chloroacetone; acetates such as amyl acetate, ethyl acetate and methyl acetate; (meth)acrylates and derivatives such as acrylamide, lauryl methacrylate and acrylonitrile; aryl compounds such as benzene, chlorobenzene, fluorobenzene, toluene, xylene, aniline and phenol; aliphatic alkanes such as pentane, isopentane, hexane, heptane and decane; halogenated alkanes such as chloroform, methylene dichloride, chloroethane and tetrachloroethylene; cyclic alkanes such as cyclopentane and cyclohexane; and polyols such as ethylene glycol, diethylene glycol, propylene glycol, hexylene glycol, and glycerol. When selecting such organic liquids for use in the solvent component of the composition, possible considerations include avoiding those which contain a functional group that will react with the acid(s) and optionally, salt(s) employed in the composition and favoring those which possess higher regulatory pre-approval limits.

(8) Preferred organic liquids include ethers and alcohols due to their low tissue toxicity and environmentally friendliness. These can be added at concentrations up to the solubility limit of the other ingredients in the composition.

(9) Ether-based liquids that can be used in the solvent component include those defined by the following general formula
R.sup.1(CH.sub.2).sub.xOR.sup.2[O(CH.sub.2).sub.z].sub.yZ(II)
where x is an integer of from 0 to 20 (optionally including, where 2x20, one or more points of ethylenic unsaturation), y is 0 or 1, z is an integer of from 1 to 4, R.sup.2 is a C.sub.1-C.sub.6 linear or branched alkylene group, R.sup.1 is a methyl, isopropyl or phenyl group, and Z is a hydroxyl or methoxy group. Non-limiting examples of glycol ethers (formula (II) compounds where Z is OH) that can be used in the solvent component are set forth below in Table 2.

(10) TABLE-US-00002 TABLE 2 Representative glycol ethers, with formula (II) variables and .sub.p values ~.sub.p R.sup.1 x R.sup.2 y z (MPa.sup.1/2) ethylene glycol monomethyl ether CH.sub.3 0 (CH.sub.2).sub.2 0 9.2 ethylene glycol monoethyl ether CH.sub.3 1 (CH.sub.2).sub.2 0 9.2 ethylene glycol monopropyl ether CH.sub.3 2 (CH.sub.2).sub.2 0 8.2 ethylene glycol monoisopropyl ether (CH.sub.3).sub.2CH 0 (CH.sub.2).sub.2 0 8.2 ethylene glycol monobutyl ether CH.sub.3 3 (CH.sub.2).sub.2 0 5.1 ethylene glycol monophenyl ether C.sub.6H.sub.5 0 (CH.sub.2).sub.2 0 5.7 ethylene glycol monobenzyl ether C.sub.6H.sub.5 1 (CH.sub.2).sub.2 0 5.9 diethylene glycol monomethyl ether CH.sub.3 0 (CH.sub.2).sub.2 1 2 7.8 diethylene glycol monoethyl ether (DGME) CH.sub.3 1 (CH.sub.2).sub.2 1 2 9.2 diethylene glycol mono-n-butyl ether CH.sub.3 3 (CH.sub.2).sub.2 1 2 7.0 propylene glycol monobutyl ether CH.sub.3 3 (CH.sub.2).sub.3 0 4.5 propylene glycol monoethyl ether CH.sub.3 1 (CH.sub.2).sub.3 0 6.5 propylene glycol monoisobutyl ether (CH.sub.3).sub.2CH 1 (CH.sub.2).sub.3 0 4.7 propylene glycol monoisopropyl ether (CH.sub.3).sub.2CH 0 (CH.sub.2).sub.3 0 6.1 propylene glycol monomethyl ether CH.sub.3 0 CH.sub.2CH(CH.sub.3) 0 6.3 propylene glycol monophenyl ether C.sub.6H.sub.5 0 CH.sub.2CH(CH.sub.3) 0 5.3 propylene glycol monopropyl ether (PGME) CH.sub.3 2 CH.sub.2CH(CH.sub.3) 0 5.6 triethylene glycol monomethyl ether CH.sub.3 0 (CH.sub.2).sub.2 2 2 7.6 triethylene glycol monooleyl ether CH.sub.3 17* (CH.sub.2).sub.2 2 2 3.1 *includes unsaturation at the 9 position

(11) Cyclic and C.sub.1-C.sub.16 acyclic (both linear and branched, both saturated and unsaturated) alcohols, optionally including one or more points of ethylenic unsaturation and/or one or more heteroatoms other than the alcohol oxygen such as a halogen atom, an amine nitrogen, and the like, can be employed as an organic liquid in the solvent component of the composition. Non-limiting examples of representative examples are compiled in the following table.

(12) TABLE-US-00003 TABLE 3 Representative alcohols, with .sub.p values ~.sub.p (MPa.sup.1/2) 2-propenol 10.8 1-butanol 5.7 t-butyl alcohol 5.1 4-chlorobenzyl alcohol 7.5 cyclohexanol 4.1 2-cyclopentenyl alcohol 7.6 1-decanol 10.0 2-decanol 10.0 2,3-dichloropropanol 9.2 2-ethyl-1-butanol 4.3 ethanol 8.8 2-ethyl-hexanol 3.3 isooctyl alcohol 7.3 octanol 3.3 methanol 12.3 oleyl alcohol 2.6 1-pentanol 4.5 2-pentanol 6.4 1-propanol 6.8 2-propanol (IPA) 6.1

(13) For further information on organic liquid-containing solvent components, the interested reader is directed to U.S. Pat. Publ. No. 2016/0073628.

(14) The composition is acidic, more particularly having a pH of no more than 4, and certain embodiments can have a pH of no more than 3.8, 3.7, 3.6, 3.5, 3.4, 3.3, 3.2, 3.1, 3.0, 2.9, 2.8, 2.7, 2.6, 2.5, 2.4, 2.3 or even 2.2. The composition has a pH of at least 1.5, generally at least 1.75, and typically at least 2.0. Ranges of pH values employing each of the lower limits in combination with each of the upper limits are envisioned. Embodiments of the composition can have pH values of 2.751.15, 2.701.05, 2.651.0, 2.600.75, 2.550.60, 2.500.55 and 2.450.45.

(15) Acidity can be achieved by adding to the solvent component (or vice versa) one or more acids. Strong (mineral) acids such as HCl, H.sub.2SO.sub.4, H.sub.3PO.sub.4, HNO.sub.3, H.sub.3BO.sub.3, and the like or organic acids, particularly organic polyacids, may be used. Examples of organic acids include monoprotic acids such as formic acid, acetic acid and substituted variants (e.g., hydroxyacetic acid, chloroacetic acid, dichloroacetic acid, phenylacetic acid, and the like), propanoic acid and substituted variants (e.g., lactic acid, pyruvic acid, and the like), any of a variety of benzoic acids (e.g., mandelic acid, chloromandelic acid, salicylic acid, and the like), glucuronic acid, and the like; diprotic acids such as oxalic acid and substituted variants (e.g., oxamic acid), butanedioic acid and substituted variants (e.g., malic acid, aspartic acid, tartaric acid, citramalic acid, and the like), pentanedioic acid and substituted variants (e.g., glutamic acid, 2-ketoglutaric acid, and the like), hexanedioic acid and substituted variants (e.g., mucic acid), butenedioic acid (both cis and trans isomers), iminodiacetic acid, phthalic acid, and the like; triprotic acids such as citric acid, 2-methylpropane-1,2,3-tricarboxylic acid, benzenetricarboxylic acid, nitrilotriacetic acid, and the like; tetraprotic acids such as prehnitic acid, pyromellitic acid, and the like; and even higher degree acids (e.g., penta-, hexa-, heptaprotic, etc.). Where a tri-, tetra-, or higher acid is used, one or more of the carboxyl protons can be replaced by cationic atoms or groups (e.g., alkali metal ions), which can be the same or different.

(16) Because of the nature of some of the defenses resulting from the various layers present in endospores, the composition must include at least one oxidizing acid. Many oxyacids, such as perchloric, chloric, chlorous, hypochlorous, persulfuric, sulfuric, sulfurous, hyposulfurous, pyrosulfuric, disulfurous, thiosulfurous, pernitric, nitric, nitrous, hyponitrous, perchromic, chromic, dichromic, permanganic, manganic, perphosphoric, phosphoric, phosphorous, hypophosphorus, periodic, iodic, iodous, etc., are considered to be oxidizing acids. Organic oxidizing acids include, but are not limited to, peracetic acid, peroxalic acid and diperoxalic acid.

(17) Preferred oxidizing acids are those which have relatively high pK.sub.a values (i.e., are not considered to be particularly strong acids) and positive standard potentials (E.sup.0.sub.red). The former permits production of a composition that has a pH value that is not too low, i.e., below 1.5, preferably not below 1.75, more preferably not below 2, and most preferably not below 2.2, so that the composition can be used without extreme protective measures by those charged with handling and applying them to surfaces and/or destroying components of articles to be treated. A positive standard potential permits the acid to have sufficient oxidizing capacity to permit overcoming or avoidance of certain endospore defenses such as, for example, oxidation of disulfide linkages and protein polymers on the endospore coat, which allows the outer spore coat to be breached.

(18) Preferred pK.sub.a values are greater than 1, greater than 1.5, greater than 2, greater than 2.5, greater than 3, greater than 3.5, greater than 4, greater than 4.5, greater than 5, and even greater than 5.5. Acids with lower pK.sub.a values can be used if other steps are taken to ensure compliance with required or desired properties of the composition such as pH range (discussed above) and effective solute concentration (discussed below).

(19) Preferred E.sup.0.sub.red values are those which are at least +0.20 V, at least +0.25 V, at least +0.33 V, at least +0.40 V, at least +0.50 V, at least +0.60 V, at least +0.67 V, at least +0.75 V, at least +0.80 V, at least +0.90 V, at least +1.00 V, at least +1.10 V, at least +1.20 V, or even at least +1.25 V.

(20) Some oxidizing acids are not particularly stable in aqueous solutions. Accordingly, providing a composition with an oxidizing acid prepared in vitro can be advantageous. For example, in one preferred embodiment, to a solvent component of a composition can be provided acetic acid and hydrogen peroxide which, when contacted, reversibly form peracetic acid.

(21) The amount of any given acid employed can be determined from the target pH of a given composition and the pK.sub.a value(s) of the chosen acids in view of the type and amounts of compound(s), if any, utilized to achieve the desired effective solute concentration in the composition. (More discussion of osmolarity and the types of osmolarity-adjusting compounds appears below.)

(22) Also present in the solute component of the composition is an electrolyte oxidizing agent that does not contain any active hydrogen atoms when subjected to a Zerewitinoff determination. Non-limiting examples of potentially useful electrolyte, preferably inorganic, oxidizing agents include compounds which include anions such as manganate, permanganate, peroxochromate, chromate, dichromate, peroxymonosulfate, and the like. (Some of these electrolytes can impact pH, so a composition formulated to have a given pH might require adjustment after addition of the oxidizing agent(s).) Preferred are those compounds having E.sup.0.sub.red values of at least +1.25 V, preferably at least +1.5 V, more preferably at least +1.75 V, even more preferably at least +2.0 V and most preferably at least +2.25 V.

(23) Electrolyte oxidizing agents generally can be added at up to their individual solubility limits, although the maximum amount generally is on the order of 30 g per liter of total composition. Exemplary ranges of electrolyte oxidizing agent(s) are 2 to 25 g/L, 3 to 21 g/L, 4 to 18 g/L, 5 to 16 g/L and 6 to 14 g/L. Exemplary amounts of electrolyte oxidizing agent(s) are 17.512 g/L, 159 g/L, 12.56 g/L and 103 g/L.

(24) Once the acid(s) and oxidizing agent(s) are added to a solvent component that contains water (or vice versa), each at least partially dissociates.

(25) A composition that includes only a solvent component and a solute component that consists, or consists essentially of, one or more oxidizing acids and one or more electrolyte oxidizing agents can have efficacy against endospores, i.e., can result in some or all endospores being rendered incapable of returning to a vegetative state. Nevertheless, a composition that includes a solute component which includes additional subcomponents can have enhanced efficacy in certain circumstances.

(26) In certain embodiments, the effective solute concentration of the composition can be relatively high. Often, efficacy increases as effective solute concentration (osmolarity) increases. The presence of an abundance of solutes ensures that a sufficient amount are present to induce a high osmotic pressure across the cortical membrane, leading to lysis.

(27) This efficacy is independent of the particular identity or nature of individual compounds of the solute component, although smaller molecules are generally more effective than larger molecules due to solvent capacity (i.e., the ability to (typically) include more small molecules in a given amount of solvent component than an equimolar amount of larger molecules) and ease of transport across cortical membranes.

(28) Any of a number of solutes can be used to increase the composition osmolarity.

(29) One approach to achieving increased osmolarity of the composition is by adding large amounts of non-oxidizing electrolytes, particularly ionic compounds (salts); see, e.g., U.S. Pat. No. 7,090,882. Like the oxidizing acid and inorganic oxidizing agent, non-oxidizing electrolytes dissociate upon being introduced into a solvent component that includes water.

(30) Where one or more organic acids are used in the composition, another approach to increasing osmolarity without increasing the pH of the composition past a desired target involves inclusion of salt(s) of one or more the acid(s) or the salt(s) of one or more other organic acids. Such compounds, upon dissociation, increase the effective amount of solutes in the composition without greatly impacting the molar concentration of hydronium ions while, simultaneously, providing a buffer system in the composition.

(31) For example, where the composition includes an acid, a fraction up to a many fold excess (e.g., 3 to 10, at least 5 or even at least 8) of one or more salts of that (or another) acid also can be included. The identity of the countercation portion of the salt is not believed to be particularly critical, with common examples including ammonium ions and alkali metals. Where a polyacid is used, all or fewer than all of the H atoms of the carboxyl groups can be replaced with cationic atoms or groups, which can be the same or different. For example, mono-, di- and trisodium citrate all constitute potentially useful buffer precursors, whether used in conjunction with citric acid or another organic acid. However, because trisodium citrate has three available basic sites, it has a theoretical buffering capacity up to 50% greater than that of disodium citrate (which has two such sites) and up to 200% greater than that of sodium citrate (which has only one such site).

(32) Regardless of how achieved, the effective solute concentration of the composition is at least 1.0 Osm/L, generally at least 1.25 Osm/L, often at least 1.5 Osm/L, commonly at least 1.75 Osm/L, more commonly at least 2.0 Osm/L, typically at least 2.25 Osm/L, more typically at least 2.5 Osm/L. (As points of comparison, in biological applications, a 0.9% (by wt.) saline solution, which is 0.3 Osm/L, typically is considered to be have moderate tonicity, while a 3% (by wt.) saline solution, which is 0.9 Osm/L, generally is considered to be hypertonic.) In some embodiments, the composition has an effective solute concentration of at least 3.0, at least 3.25, at least 3.5, at least 3.75, or even at least 4.0 Osm/L, with the upper limit being defined by the solubility limit of the solutes in the solvent component; in some embodiments, the upper limit of effective solute concentration can range as high as 4.5, 4.75, 5.0, 5.25, 5.5, 5.75, 6.0, 6.25, 6.5, 6.75, 7.0, 7.2, 7.4, 7.6, 7.8, 8.0, 8.2, 8.4, 8.6, 8.8 or even 9 Osm/L. Effective solute concentration ranges involving combinations of any of the lower and upper limits set forth in this paragraph also are envisioned. The effective solute concentrations of compositions according to the present invention, which are intended to be effective against (i.e., lethal to) endospores, generally are higher than those described in U.S. Pat. Nos. 8,940,792 and 9,314,017 as well as U.S. Pat. Publ. Nos. 2010/0086576, 2013/0272922, 2013/0079407 and 2016/0073628, all of which are directed generally against planktonic bacteria and biofilms.

(33) Effective solute concentration can be calculated using known techniques or, if desired, measured using any of a variety of colligative property measurements such as boiling point elevation, freezing point depression, osmotic pressure and lowering of vapor pressure.

(34) Unlike many of the compositions described in the documents listed in the preceding paragraph, the present sporicidal composition does not require inclusion of surfactant in the solute component, although certain preferred embodiments include one or more wetting agents which include, but are not limited to, surfactants.

(35) Essentially any material having surface active properties in water can be employed, regardless of whether water is present in the solvent component of the composition, although those surface active agents that bear some type of ionic charge are expected to have enhanced antimicrobial efficacy because such charges, when brought into contact with a bacteria, are believed to lead to more effective bacterial membrane disruption and, ultimately, to cell leakage and lysis.

(36) Polar surfactants generally are more efficacious than non-polar surfactants, with ionic surfactants being most effective. For polar surfactants, anionic surfactants generally are the most effective, followed by zwitterionic and cationic surfactants, with smaller molecules generally being preferred over larger ones. The size of side-groups attached to the polar head can influence the efficacy of ionic surfactants, with larger size-groups and more side-groups on the polar head potentially decreasing the efficacy of surfactants.

(37) Potentially useful anionic surfactants include, but are not limited to, ammonium lauryl sulfate, dioctyl sodium sulfosuccinate, perflourobutanesulfonic acid, perfloruononanoic acid, perfluorooctanesulfonic acid, perfluorooctanoic acid, potassium laurylsulfate, sodium dodeylbenzenesulfonate, ladium laureth sulfate, sodium lauroyl sarcosinate, sodium myreth sulfate, sodium myreth sulfate, sodium pareth sulfate, sodium stearate, sodium chenodeoxycholate, N-lauroylsarcosine sodium salt, lithium dodecyl sulfate, 1-octanesulfonic acid sodium salt, sodium cholate hydrate, sodium deoxycholate, sodium dodecyl sulfate (SDS), sodium glycodeoxycholate, sodium lauryl sulfate (SLS), and the alkyl phosphates set forth in U.S. Pat. No. 6,610,314. SLS is a particularly preferred material.

(38) Potentially useful cationic surfactants include, but are not limited to, cetylpyridinium chloride (CPC), cetyl trimethylammonium chloride, benzethonium chloride, 5-bromo-5-nitro-1,3-dioxane, dimethyldioctadecylammonium chloride, cetrimonium bromide, dioctadecyldimethylammonium bromide, tretadecyltrimethyl ammonium borine, benzalkonium chloride (BK), hexadecylpyridinium chloride monohydrate and hexadecyltrimethylammonium bromide.

(39) Potentially useful nonionic surfactants include, but are not limited to, sodium polyoxyethylene glycol dodecyl ether, N-decanoyl-N-methylglucamine, digitonin, n-dodecyl -D-maltoside, octyl -D-glucopyranoside, octylphenol ethoxylate, polyoxyethylene (8) isooctyl phenyl ether, polyoxyethylene sorbitan monolaurate, and polyoxyethylene (20) sorbitan cholamidopropyl) dimethylammonio]-2-hydroxy-1-propane sulfonate, 3-[(3-cholamidopropyl) dimethylammonio]-1-propane sulfonate, 3-(decyldimethylammonio) propanesulfonate inner salt, and N-dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate.

(40) Potentially useful zwitterionic surfactants include sulfonates (e.g. 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate), sultaines (e.g. cocamidopropyl hydroxysultaine), betaines (e.g. cocamidopropyl betaine), and phosphates (e.g. lecithin).

(41) For other potentially useful materials, the interested reader is directed to any of a variety of other sources including, for example, U.S. Pat. Nos. 4,107,328, 6,953,772, 7,959,943, and 8,940,792.

(42) The amount(s) of wetting agent(s) to be added to the composition is limited to some extent by the target effective solute concentration and compatibility with other subcomponents of the solute component of the composition. The total amount of wetting agent present in the composition can range from at least 0.1%, from at least 0.25%, from at least 0.5%, from at least 0.75% or from at least 1% up to 5%, commonly up to 4%, more commonly up to 3%, and typically up to 2.5%. At times, maximum amounts of certain types of wetting agents, particularly surfactants, that can be present in a composition for a particular end use (without specific testing, review and approval) are set by governmental regulations.

(43) If more than one type of surfactant is employed, the majority preferably is an ionic surfactant, with the ratio of ionic-to-nonionic surfactant generally ranging from 2:1 to 10:1, commonly from 5:2 to 15:2, and typically from 3:1 to 7:1. Additionally, as is known in the art, a composition should not include surfactant types that are incompatible, e.g., anionic with cationic or zwitterionic with either anionic or cationic.

(44) The antimicrobial composition can include a variety of additives and adjuvants to make it more amenable for use in a particular end-use application with negatively affecting its efficacy in a substantial manner. Examples include, but are not limited to, emollients, fungicides, fragrances, pigments, dyes, defoamers, foaming agents, flavors, abrasives, bleaching agents, preservatives (e.g., antioxidants) and the like. A comprehensive listing of additives approved by the U.S. Food and Drug Administration is available as a zipped text file at http://www.fda.gov/Drugs/InformationOnDrugs/ucm113978.htm (link active as of filing date of this application).

(45) The composition's efficacy does not require the inclusion of an active antimicrobial agent (defined above) for efficacy, but such materials can be included in certain embodiments. Non-limiting examples of potentially useful active antimicrobial additives include C.sub.2-C.sub.8 alcohols (other than or in addition to any used as an organic liquid of the solvent component) such as ethanol, n-propanol, and the like; aldehydes such as gluteraldehyde, formaldehyde, and o-phthalaldehyde; formaldehyde-generating compounds such as noxythiolin, tauroline, hexamine, urea formaldehydes, imidazolone derivatives, and the like; anilides, particularly triclocarban; biguanides such as chlorhexidine and alexidine, as well as polymeric forms such as poly(hexamethylene biguanide); dicarboximidamides (e.g., substituted or unsubstituted propamidine) and their isethionate salts; halogen atom-containing or releasing compounds such as bleach, ClO.sub.2, dichloroisocyanurate salts, tosylchloramide, iodine (and iodophors), and the like; silver and silver compounds such as silver acetate, silver sulfadiazine, and silver nitrate; phenols, bis-phenols and halophenols (including hexachlorophene and phenoxyphenols such as triclosan); and quaternary ammonium compounds.

(46) The following tables provide ingredient lists for exemplary compositions according to the present invention, with amounts being provided in grams and with distilled water being added to bring the ingredients to a volume of 1 L.

(47) TABLE-US-00004 TABLE 4 Formulations for exemplary compositions Formulation 1 Formulation 2 salt of organic acid 5-25 10-20 organic acid 125-200 140-180 ionic surfactant 5-30 15-25 nonionic surfactant 0-5 1-3 H.sub.2O.sub.2 (30% by wt. in H.sub.2O) 175-325 200-300 inorganic oxidizing agent 4-20 6-12 organic liquid 175-450 225-375

(48) Various embodiments of the present invention have been provided by way of example and not limitation. As evident from the foregoing tables, general preferences regarding features, ranges, numerical limitations and embodiments are, to the extent feasible and as long as not interfering or incompatible, envisioned as being capable of being combined with other such generally preferred features, ranges, numerical limitations and embodiments.

(49) A composition according to the present invention is intended to be, and in practice is, aggressively antimicrobial. Its intended usages are in connection with inanimate objects such as, in particular, hard surfaces, particularly those commonly found in healthcare facilities.

(50) The composition can be applied to inanimate objects, particularly hard surfaces, in a variety of ways including pouring, spraying or misting, via a distribution device (e.g., mop, rag, brush, textile wipe, etc.), and the like.

(51) Alternatively, certain objects are amenable to being immersed in a composition. This is particularly true of medical equipment designed for use with multiple patients such as, for example, dialysis equipment, any of a variety of endoscopes, duodenoscopes, etc., endoscopic accessories such as graspers, scissors and the like, manual instruments such as clamps and forceps, laparoscopic surgical accessories, orthopedic and spinal surgery hardware such as clamps and jigs, and the like. Because the composition of the present invention has a more moderate [H.sup.+] than treatments such as peracetic acid and bleach, it can achieve disinfection, high level disinfection or even sterilization without negative effects such as, e.g., polymeric degradation, metal corrosion, glass or plastic etching, and the like.

(52) Once applied to a surface or object, the various ingredients of the composition act on any endospores present and avoid or break down their various defenses. The contact time necessary for a composition to treat endospores (i.e., ensure that they cannot return to a vegetative state) can vary widely depending on the particular composition and its intended end use.

(53) For example, embodiments of a composition intended to be applied to hard surfaces in a healthcare facility can achieve at least a 3.0, 3.2, 3.4, 3.6, 3.8, 4.0, 4.2, 4.4, 4.6, 4.8 or 5.0 log reduction after a contact time of more than 1200 seconds, no more than 1050 seconds, no more than 900 seconds, no more than 840 seconds, no more than 780 seconds, no more than 720 seconds, no more than 660 seconds, or even no more than 600 seconds. When tested in accordance with ASTM E2197-11, certain embodiments can achieve at least a 4.5 log reduction after a contact time of 600 seconds.

(54) These and/or other embodiments of a composition intended for use as a soaking bath for medical devices can achieve at least a 5.0, 5.1, 5.2, 5.3, 5.4, 5.5, 5.6, 5.7, 5.8, 5.9, or 6.0 log reduction after a contact time of 14,400 seconds, up to 10,800 seconds, up to 7200 seconds, up to 5400 seconds, and on the order of 3600 seconds. When tested in accordance with AOAC Official Method 966.04, certain embodiments can achieve a passing score after contact times as low as 1800 seconds.

(55) Embodiments of the sporicidal composition may be able to be classified as high level disinfectants or even as sterilants.

(56) After the composition has been allowed to contact a given object or surface for an appropriate time (in view of factors such as expected bacterial load, type of bacteria potentially present, importance of the object/surface, etc.), it can be left to evaporate or, preferably, rinsed away with water or a dilute saline solution.

(57) The following non-limiting, illustrative examples provide detailed conditions and materials that can be useful in the practice of the present invention. Throughout those examples, any reference to room temperature refers to 22 C.

EXAMPLES

Example 1 (Comparative): Peracetic Acid

(58) A widely recognized and recommended disinfection treatment where endospores are possible or suspected is application to the target surface and 10 minute contact time of 10% (w/v) peracetic acid. Accordingly, peracetic acid constitutes a good comparative for compositions of the present invention.

(59) Two peracetic acid solutions were prepared by adding distilled water to a flask containing peracetic acid. The concentration of one of the solutions was 5% (w/v) while that of the other was 10% (w/v). The calculated effective solute concentrations for these solutions were 1.97 and 3.95 Osm/L, respectively.

(60) To a 50 mL beaker was added 20 mL distilled water. A cleaned, rinsed and dried probe from a calibrated, temperature compensating pH meter was lowered into the beaker. Sequential aliquots of the peracetic acid solution then were added to the beaker, with pH readings after each. The titrations were performed at room temperature.

(61) The results of these titrations are shown below in Table 5.

(62) TABLE-US-00005 TABLE 5 Peracetic acid titrations 5% (w/v) 10% (w/v) Amt. acid, Amt. acid, mL pH mL pH 0 5.91 0 5.31 0.25 2.42 0.1 2.74 0.50 2.17 0.2 2.48 0.75 2.00 0.3 2.33 1.00 1.89 0.4 2.22 1.25 1.80 0.5 2.14 1.50 1.74 0.6 2.07 1.75 1.68 0.7 2.00 2.00 1.59 0.8 1.95 2.25 1.56 0.9 1.91 2.50 1.53 1.0 1.86 2.75 1.48 1.1 1.83 3.00 1.45 1.2 1.79 3.25 1.42 1.3 1.76 3.50 1.41 1.4 1.73 3.75 1.38 1.5 1.70 4.00 1.34 1.6 1.67 4.25 1.30 1.7 1.65 4.50 1.28 1.8 1.62 4.75 1.26 1.9 1.60 5.00 1.25 2.0 1.58

(63) The above data indicate, inter alia, that the pH of water is reduced to below 3 upon addition of even a tiny aliquot, i.e., less than 1% by volume, of either peracetic acid solution and that the addition of only 1 mL (5% by volume) of either solution has reduced the pH to below 2. Further, the asymptotic pH for either acid solution is on the order of 1.10.1.

(64) Further, U.S. EPA recommendations are for peracetic acid solutions of at least 2.5% (w/v) which, according to the tabulated data, has a pH of no more than 1.25.

(65) Thus, any worker performing this recommended disinfection procedure (i.e., application of a 2.5-10% peracetic acid solution) should employ the types of precautions appropriate for handling strong acids such as, e.g., protective gloves, protective eyewear, breathing masks, etc.

Example 2 (Comparative): Bleach

(66) Another widely recognized and recommended disinfection treatment where endospores are possible or suspected is application to the target surface and 10 minute contact time of a bleach solution.

(67) To a 50 mL beaker was added 5 mL of a bleach solution, i.e., 8.25% (w/v) sodium hypochlorite. A cleaned, rinsed and dried probe from a calibrated, temperature compensating pH meter was lowered into the beaker. Sequential aliquots of distilled water then were added to the beaker, with pH readings after each.

(68) The data from this titration are shown below in Table 6.

(69) TABLE-US-00006 TABLE 6 Titration of household bleach with water Water, [ClO.sup.], % mL (w/v) pH 0 8.25 12.56 2 5.89 12.46 4 4.58 12.33 6 3.75 12.23 8 3.17 12.14 10 2.75 12.06 12 2.43 12.00 14 2.17 11.94 16 1.96 11.89 18 1.79 11.84 20 1.65 11.79 22 1.53 11.74 24 1.42 11.70 25 1.38 11.68

(70) The data of this table indicate, inter alia, that an undiluted 8.25% bleach solution has a pH of almost 13 and that reducing the ClO.sup. concentration by three-fourths reduces this only to 11.9. Conversely, reducing the concentration from 8.25% to 5% (both w/v) results in the calculated effective solute concentration being reduced by almost half, i.e., from 2.28 to 1.34 Osm/L.

(71) Thus, any worker performing this recommended disinfection procedure (i.e., application of a bleach solution) should employ the types of precautions appropriate for handling strong bases, e.g., protective gloves, protective eyewear, breathing masks, etc.

Examples 3-10: In Vitro Time-to-Kill

(72) Efficacy of certain sporicidal compositions was performed against Clostridium difficile (ATCC #43598). In this testing, reduction of bacteria is determined by comparison against untreated controls (employing phosphate buffered saline as liquid) at various time test points, typically equal increments such as 15 minutes (900 seconds).

(73) A 9.9 mL aliquot of the solution to be tested was placed in a 20 mL test tube. A 0.1 mL volume of the test culture (10.sup.6 colony forming units (CFU) of C. Diff per mL when diluted) was added to the test tube, which then was vortexed. After a predetermined amount of contact time, 1.0 mL of the sample/test culture suspension was transferred into sterile test tubes containing 9.0 mL of an appropriate neutralization solution, followed by additional vortexing.

(74) Serial tenfold dilutions then were prepared by transferring 0.5 mL aliquots of test solution into 4.5 mL of neutralizing solution, with vortex mixing between dilutions. From these dilutions, duplicate 1.0 mL aliquots were spread-plated onto brain-heart agar plates, which then were incubated anaerobically at 352 C. for 72 hours.

(75) Following incubation, the colonies on the plates were counted, with counts in the 20 to 200 CFU range used in data calculations. The log reduction from this testing is performed by subtracting the CFU/mL recovered treatment value from the CFU/mL recovered control sample.

(76) A number of compositions were tested in this manner, with the time to achieve 6 log reductions in spores shown in the last column of Table 7 below. Each of the compositions was prepared based on a targeted 2.3 Osm/L effective solute concentration and a target pH of 4. (In the buffer system column, A represents acetic acid/sodium acetate, while C represents citric acid/trisodium citrate. In the oxidant column, PPOMS represents peroxymonosulfate, all at 0.22% (w/v), and PAA represents peracetic acid at the noted concentration.) Those compositions designated as employing BK as a surfactant included 0.21% (w/v), while those designated as employing SDS included 0.175% (w/v). For those compositions showing inclusion of an organic liquid, an isopropanol solution (70% in water) was employed.

(77) TABLE-US-00007 TABLE 7 Buffer Org. liquid Time Example system Oxidant Surfactant (%, w/v) (min.) 3 A PPOMS BK 30 4 A PPOMS SDS 30 5 A PPOMS BK 10.0 60 6 C PAA (0.1%) BK 20.0 30 7 C PAA (1.0%) BK 20.0 30 8 A PPOMS BK 30 9 A PPOMS SDS 30 10 A PPOMS SDS 10.0 15

(78) The times shown in the foregoing table are better than those which can be achieved with most commercially available sporicidal products, which typically require 240 to 2160 minutes for C. Diff disinfection. Additionally, exposure to each of these compositions is far less dangerous than exposure to such commercial products.

Examples 11-22

(79) The data from Table 7 above seem to indicate that compositions employing an anionic surfactant (SDS) might provide better results than those employing a cationic surfactant (BK).

(80) To further investigate efficacy, additional compositions were prepared, each of which employed the same amount of SDS as was used in Examples 4 and 9-10 plus 0.02% (w/v) of a polysorbate-type nonionic surfactant. Each also included 250 g/L of a 30% H.sub.2O.sub.2 solution and 300 g/L of an organic liquid. The electrolyte oxidizing agent (EOA) for each composition was PPOMS. The citric acid-containing compositions included 140 g/L citric acid and 17.5 g/L trisodium citrate dihydrate (along with the noted amounts of NaCl to raise the effective solute concentration to a predetermined target), while the acetic acid-containing compositions included the noted amounts of acetic acid (AA) and sodium acetate (SA).

(81) Quantitative carrier testing was performed in substantial accord with ASTM standard E2197-11, with efficacy against spores being shown in the last columns of the following tables.

(82) TABLE-US-00008 TABLE 8a citric acid compositions Amt. NaCl Amt. EOA Organic Log Example Target pH (g/L) (g/L) liquid reduction 11 1.5 54.0 12.0 IPA 5.6 12 3.5 54.0 4.0 IPA 3.2 13 1.5 178.2 12.0 DGME <3 14 3.5 178.2 4.0 DGME <3 15 2.5 116.0 8.0 DGME 3.9 16 2.5 116.0 8.0 IPA 3.9

(83) TABLE-US-00009 TABLE 8b acetic acid compositions Amts. AA/SA Amt. EOA Organic Log Example Target pH (g/L) (g/L) liquid reduction 17 1.5 82.4/7.4 4.0 DGME 5.0 18 3.5 82.4/7.4 12.0 DGME 3.3 19 1.5 164.9/14.8 4.0 IPA 5.1 20 3.5 164.9/14.8 12.0 IPA 4.7 21 2.5 123.6/11.1 8.0 DGME 5.3 22 2.5 123.6/11.1 8.0 IPA 5.0

(84) Statistical analysis of the data from these tables suggested that the type of acid has the greatest impact on efficacy followed by, in order, the pH (lower being better), type of solvent, and effective solute concentration. The amount of electrolyte oxidizing agent appears to have a lesser effect.

Examples 23-31

(85) Using Example 22 as a center point (rerun below as Example 23), additional quantitative carrier tests were conducted on another round of prepared compositions in which the targeted pH (2.5), effective solute concentration (6.4 Osm/L) and amount of PPOMS (8 g/L) were held constant. The anionic surfactant was SDS, while the nonionic surfactant was a polysorbate. The organic liquid for each was a 70% (v/v) IPA solution.

(86) TABLE-US-00010 TABLE 9 Org. Anionic liquid H.sub.2O.sub.2 soln. surf. Nonionic surf. Log Example (g/L) (g/L) (g/L) (g/L) reduction 23 250 250 17.5 2.0 4.6 24 200 200 15.0 1.5 4.6 25 400 300 15.0 1.5 4.7 26 200 300 20.0 1.5 4.6 27 400 200 20.0 1.5 4.6 28 200 300 15.0 2.5 4.7 29 400 200 15.0 2.5 4.6 30 200 200 20.0 2.5 4.5 31 400 300 20.0 2.5 4.7

(87) Analysis of the data for Examples 11-31 indicated that pH and type of acid had the greatest impact, followed by effective solute concentration and type of solvent.

Examples 32-40

(88) Additional quantitative carrier testing was performed on compositions in which the pH (2.5) and effective solute concentration (6.4 Osm/L) were held constant. This set varied the amount of electrolyte oxidizing agent (PPOMS), the amount and type of solvent (with E representing absolute ethanol), the amount of hydrogen peroxide solution, and the amounts of anionic (SDS) and nonionic (polysorbate-type) surfactants.

(89) TABLE-US-00011 TABLE 10 Org. H.sub.2O.sub.2 Anionic Nonionic Exam- liquid soln. PPOMS surf. surf. Log ple (g/L) (g/L) (g/L) (g/L) (g/L) reduction 32 E, 250 200 12 15.0 1.5 4.7 33 E, 250 300 20 15.0 1.5 4.7 34 DGME, 250 300 12 20.0 1.5 4.5 35 DGME, 250 200 20 20.0 1.5 3.9 36 E, 350 300 12 15.0 2.5 3.8 37 E, 350 200 20 15.0 2.5 4.5 38 DGME, 350 200 12 20.0 2.5 4.6 39 DGME, 350 300 20 20.0 2.5 4.7 40 IPA, 300 250 16 17.5 2.0 4.7

(90) Analysis of this data suggests that, when type and amount of acid is held constant, the most statistically significant factors might be two-way combinations type of solvent, solvent concentration, and amount of electrolyte oxidizing agent.