COMPOSITION

Abstract

The present invention relates to compositions for use and formulation as weight management products and in particular fermented dairy foodstuffs. The compositions generally comprise two or more of the following components: a) a microbiome modifying component; b) a satiety modifying component; and c) a metabolic modifying component and at least one of the following: d) Streptococcus thermophilus CBS 139100 and Lactobacillus delbrueckii subsp. bulgaricus CBS 139099 microbial strains or mutant strains or fragments or fractions thereof; e) a combination of Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus microbial strains which together, or individually, are capable of secreting polysaccharides having high levels of galactosamine and no or low levels of rhamnose and glucuronic acid when grown on a dairy substrate; or f) polysaccharides formed of: i) 15-25% galactosamine; ii) 45-60% galactose; and iii) 20-30% glucose. The compositions may also be useful in the management or treatment of obesity, elevated cholesterol, diabetes, hypertension or heart disease.

Claims

1. A composition comprising two or more of the following components: a) a microbiome modifying component; b) a satiety modifying component; and c) a metabolic modifying component, and at least one of following: d) Streptococcus thermophilus CBS 139100 and Lactobacillus delbrueckii subsp. bulgaricus CBS 139099 microbial strains or fragments or fractions thereof; e) a combination of Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus microbial strains which together, are capable of secreting polysaccharides having high levels of galactosamine and no or low levels of rhamnose and glucuronic acid when grown on a dairy substrate; or f) polysaccharides formed of: i) 15-25% galactosamine; ii) 45-60% galactose; and iii) 20-30% glucose.

2. The composition of claim 1, wherein the composition comprises polysaccharides having high levels of galactosamine and no or low levels of rhamnose and glucuronic acid.

3. The composition of claim 1, wherein the polysaccharides comprise 45-60% galactose, 20-30% glucose and 15-25% galactosamine, based on the total weight of secreted exopolysaccharides.

4. The composition of claim 1, wherein the composition comprisesStreptococcus thermophilus CBS 139100 and Lactobacillus delbrueckii subsp. bulgaricus CBS 139099 microbial strains.

5. The composition of claim 1, wherein the composition comprises f) and further comprises Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus microbial strains.

6. (canceled)

7. (canceled)

8. (canceled)

9. The composition of claim 1, wherein the microbiome modifying component comprises a growth medium, a selective microbial growth inhibitor, a micro-biocidal compound or a combination thereof.

10. The composition of claim 8, wherein the growth medium comprises a prebiotic.

11. (canceled)

12. (canceled)

13. The composition of claim 1, wherein the microbiome modifying component comprises a prebiotic; the satiety modifying component comprises dietary fibre and the metabolic modifying component comprises chromium.

14. The composition of claim 13, wherein the dietary fibre comprises glucomannan and/or a prebiotic comprising one or more selected from: inulin, fructooligosaccharides (FOS), galactooligosaccharides (GOS), -gluco-oligosaccharides, a low gas producing prebiotic and combinations thereof.

15. The composition of claim 13, wherein the composition comprises the dietary fibre in the range of 0.5 to 15 g.

16. The composition of claim 13, wherein the composition comprises chromium in the range of 50 to 1000 g.

17. The composition of claim 13, wherein the composition comprises a prebiotic in the range of 3 to 30 g.

18. A composition comprising a combination of: a) glucomannan and chromium; b) glucomannan, FOS and chromium; c) glucomannan and FOS; or d) glucomannan and a low gas producing prebiotic.

19. The composition of claim 18, wherein the composition is grown on a dairy substrate, is formed as part of a dairy substrate or product or is used as a feedstock, ingredient or additive for use with a fermented or fermentable dairy foodstuff.

20. The composition of claim 19, wherein the fermented or fermentable dairy foodstuff is selected from one or more of the following: yoghurt, cheese, crme fraiche, sour cream, buttermilk, kefir, acidophilus milk, koumiss, filmjlk and viili.

21. The composition of claim 19, wherein the composition further comprises one or more ingredients selected from: sweeteners, stabilisers, acidity regulators, water, flavourings, fats, emulsifiers, plant and/or vegetable extracts, vitamins, minerals, phytochemicals, antioxidants, and combinations thereof.

22. The composition of claim 18, wherein the composition comprises: a) 50 to 90% fermented or fermentable dairy food stuff comprising and/or fermented using Streptococcus thermophilus CBS 139100 and Lactobacillus delbrueckii subsp. bulgaricus CBS 139099; b) 1 to 10% sweetener; c) 5 to 20% GOS; d) up to 1.5% glucomannan; e) up to 0.010% chromium chloride; and optionally f) 5 to 30% fruit preparation.

23. A method for treatment or management of weight, elevated cholesterol, diabetes, hypertension or heart disease in a subject comprising providing the subject with the composition of claim 1.

24. (canceled)

25. (canceled)

26. (canceled)

27. (canceled)

28. (canceled)

29. (canceled)

30. (canceled)

31. (canceled)

32. The method of claim 23 wherein the method provides for the management of weight.

33. The method of claim 23 wherein the method provides for the treatment of diabetes.

Description

EXAMPLES

[0143] The figures accompanying the below examples are as follows:

[0144] FIG. 1 is a graph showing the viscosity, laser scattering, UV absorption at 280 nm and differential refractive index of the exopolysaccharides of yoghurt 16;

[0145] FIG. 2 is a graph showing molar mass plotted against time for yoghurts 14b and 16;

[0146] FIG. 3 is a graph showing hydrodynamic radius plotted against time for yoghurts 14b and 16;

[0147] FIG. 4 is a graph showing intrinsic viscosity plotted against time for the yoghurts 14b and 16;

[0148] FIG. 5 is a graph showing an Rh conformation plot with hydrodynamic radius plotted against molar mass for yoghurts 14b and 16; and

[0149] FIG. 6 shows photographs of three jars of yoghurt prepared according to the recipes outlined in the yoghurt Example A, in which (moving left to right) the yoghurt products produced were (1) fruit on the bottom, (2) fruit mixed in as pieces and (3) fruit mixed in homogeneous.

[0150] Experiments were conducted to prepare a weight management formulation in the form of yoghurt. Example 1A describes the formation of a base yoghurt which was then used to prepare a finished weight management yoghurt as outlined in Example 1B.

Example 1AInitial Yoghurt Component

[0151] Yoghurts were prepared using the bacterial strains Streptococcus thermophilus (NIZO2274=Cell Deposit Ref: CBS 139100) and Lactobacillus delbrueckii subsp. bulgaricus (NIZO2118=Cell Deposit Ref: CBS 139099). Placebo yoghurts were also prepared using the bacterial strains Streptococcus thermophilus NIZO115 and Lactobacillus delbrueckii subsp. bulgaricus NIZO191; Streptococcus thermophilus NIZO131 and Lactobacillus delbrueckii subsp. bulgaricus NIZO191; and Streptococcus thermophilus NIZO131 and Lactobacillus delbrueckii subsp. bulgaricus NIZO194. The ingredients fermented by the bacterial cultures were 96.3% skimmed milk (w/w) and 3.7% cream (w/w). After formation of the yoghurts the pH and viscosity of the yoghurts was measured.

[0152] As shown in table 2 below yoghurts prepared using Streptococcus thermophilus NIZO2274 and Lactobacillus delbrueckii subsp. bulgaricus NIZO2118 had a greater viscosity than the placebo yoghurts. The yoghurts prepared using Streptococcus thermophilus NIZO2274 and Lactobacillus delbrueckii subsp. bulgaricus NIZO2118 also had a greater viscosity than yoghurts which included known thickening agents such as k-carrageenan, cekol (cellulose gum), guar (gum) and pectin.

TABLE-US-00002 TABLE 2 pH after cultivation Viscosity Sample Additive and cooling (sec postumus) S.therm NIZO115 + L.bulg. NIZO191 4.15 7 ISt concentrate 4.24 14 RR concentrate 4.27 38 S.therm NIZO131 + L.bulg. NIZO191 4.13 14 S.therm NIZO131 + L.bulg. NIZO194 4.15 43 S.therm NIZO2274 + L.bulg. NIZO2118 4.33 83 S.therm NIZO131 + L.bulg. NIZO194 0.1% K-car 4.28 19 S.therm NIZO131 + L.bulg. NIZO194 0.25% cekol 4.28 13 S.therm NIZO131 + L.bulg. NIZO194 0.25% guar 4.27 11 S.therm NIZO131 + L.bulg. NIZO194 0.25% pectin 4.16 46 S.therm NIZO115 + L.bulg. NIZO191 4.15 6 (6/6) RR concentrate 4.24 47 (48/46) S.therm NIZO131 + L.bulg. NIZO191 4.14 19 (18/19) S.therm NIZO131 + L.bulg. NIZO194 4.28 55 (55/54) S.therm NIZO2274 + L.bulg. NIZO2118 4.42 69 (69/68) S.therm NIZO131 + L.bulg. NIZO194 0.25% pectin 4.26 30 (30/30)

[0153] As shown in table 3 after 5 days storage at 4 C. the yoghurt prepared using Streptococcus thermophilus NIZO2274 and Lactobacillus delbrueckii subsp. bulgaricus NIZO2118 had a greater viscosity than the placebo yoghurt. The placebo yoghurt also had a coarse texture whereas the yoghurt prepared using Streptococcus thermophilus NIZO2274 and Lactobacillus delbrueckii subsp. bulgaricus NIZO2118 had a smooth texture.

TABLE-US-00003 TABLE 3 Viscosity (sec postumus) Yoghurt (20 C.) pH Structure S.therm NIZO2274 + L.bulg. NIZO2118 77 4.30 Smooth S.therm NIZO115 + L.bulg. NIZO191 7 4.01 Coarse, with pieces

[0154] The exopolysaccharides secreted by the placebo culture Streptococcus thermophilus NIZO115 and Lactobacillus delbrueckii subsp. bulgaricus NIZO191 and by strains Streptococcus thermophilus NIZO2274 and Lactobacillus delbrueckii subsp. bulgaricus NIZO2118 were characterised. The placebo yoghurt prepared using strains Streptococcus thermophilus NIZO115 and Lactobacillus delbrueckii subsp. bulgaricus NIZO191 was labelled Yoghurt 14b, SATIN 1A. The yoghurt prepared using Streptococcus thermophilus NIZO2274 and Lactobacillus delbrueckii subsp. bulgaricus NIZO2118 was labelled Yoghurt 16, SATIN 5A. Tables 4, 5 and 6 below show the exopolysaccharide amounts, percentages and ratios in the yoghurts.

[0155] Tables 4, 5 and 6 show the differences in the complements of exopolysaccharides secreted by the different bacterial strains. Yoghurt 16 comprises a greater amount of galactosamine, galactose and glucose than yoghurt 14b. Yoghurt 14b comprises more rhamnose, mannose and glucuronic acid than yoghurt 16.

TABLE-US-00004 TABLE 4 Amount Amount Amount Amount Total Amount Galacto- Amount Gluco- Amount Amount Amount Amount Galactu- Glucu- mono- Rhamnose samine Arabinose samine Galactose Glucose Mannose Xylose ronic acid ronic acid sacch. Begin End ED_1 ED_1 ED_1 ED_1 ED_1 ED_1 ED_1 ED_1 ED_1 ED_1 ED_1 Sample Peak Peak [mg/g [mg/g [mg/g [mg/g [mg/g [mg/g [mg/g [mg/g [mg/g [mg/g [mg/g Name [min] [min] EPS] EPS] EPS] EPS] EPS] EPS] EPS] EPS] EPS] EPS] EPS] A10, AH, 25,0 27,0 197 0 0 0 15 171 65 0 0 85 533 Yoghurt 14b, SATIN 1A, fraction 3 A11, AH, 25,0 27,0 0 166 0 0 402 200 4 0 0 0 771 Yoghurt 16, SATIN 5A, fraction 3

TABLE-US-00005 TABLE 5 Amount Amount Amount Amount Total Amount Galacto- Amount Gluco- Amount Amount Amount Amount Galactu- Glucu- mono- Begin End Rhamnose samine Arabinose samine Galactose Glucose Mannose Xylose ronic acid ronic acid sacch. Sample Peak Peak ED_1 ED_1 ED_1 ED_1 ED_1 ED_1 ED_1 ED_1 ED_1 ED_1 ED_1 Name [min] [min] [%] [%] [%] [%] [%] [%] [%] [%] [%] [%] [%] A10, AH, 25,0 27,0 37 0 0 0 2,7 32,1 12,2 0 0 16 100 Yoghurt 14b, SATIN 1A, fraction 3 A11, AH, 25,0 27,0 0 21,5 0 0 52,1 25,9 0,5 0 0 0 100 Yoghurt 16, SATIN 5A, fraction 3

TABLE-US-00006 TABLE 6 Amount Amount Amount Amount Amount Galacto- Amount Gluco- Amount Amount Amount Amount Galactu- Glucu- Begin End Rhamnose samine Arabinose samine Galactose Glucose Mannose Xylose ronic acid ronic acid Sample Peak Peak ED_1 ED_1 ED_1 ED_1 ED_1 ED_1 ED_1 ED_1 ED_1 ED_1 Name [min] [min] [ratio] [ratio] [ratio] [ratio] [ratio] [ratio] [ratio] [ratio] [ratio] [ratio] A10, AH, 25,0 27,0 14 0 0 0 1 12 4 0 0 6 Yoghurt 14b, SATIN 1A, fraction 3 A11, AH, 25,0 27,0 0 1 0 0 2 1 0 0 0 0 Yoghurt 16, SATIN 5A, fraction 3

[0156] Size exclusion chromatography with refractive index, UV (280 nm, protein, polyphenols), viscosity and multi angle laser light scattering (MALLS) detection was used to determine the concentration, molar mass (Mw), radius Rh(w) and intrinsic viscosity [n]w of yoghurts 14b and 16.

[0157] The columns used were TSK gel PWXL Guard+TSK gel G6000 PWXL+TSK gel G5000 PWXL. The eluent (mobile phase) was 100 mM sodium nitrate, NaNO.sub.3, +0.02% sodium azide, NaN3. The flow rate was 0.500 mL/min. The column temperature was 35 C. The temperature used for the determination of the laser scattering, viscosity and refractive index was 35 C. The injection volume was 200 l. The freeze dried samples (0.05 g) were weighed into a 20 ml bottle. 2.0 ml of eluent was added to the bottle and the sample was stirred overnight (16 hours) at room temperature. An ultrasonic bath was not used as this would break down the exopolysaccharides. The solutions were centrifuged for 10 minutes at 20,000 g in a 2 ml centrifuge tube. The solution was then filtered using a 0.22 m filter (Millex-GV, low protein binding Durapore, PVDF, Cat no.:SLGVX13NL (Millipore)). 200 l of the filtrated supernatant was then injected into the size exclusion chromatography column. The runtime injector was 131 minutes. The detection of the refractive index can indicate dissolved substances, minerals, lactose, proteins, casein micelles and solids content.

[0158] FIG. 1 shows the viscosity, light scattering, refractive index and UV absorption at 280 nm of yoghurt 16.

[0159] FIG. 2 shows yoghurt 16 has a greater molar mass compared to the placebo yoghurt 14b. FIG. 3 and table 9 show yoghurt 16 has a greater hydrodynamic radius compared to the placebo yoghurt 14b. FIG. 4 and table 9 show yoghurt 16 has a greater intrinsic viscosity than the placebo yoghurt 14b.

[0160] FIG. 5 shows the relationship between the hydrodynamic radius and molar mass for yoghurts 16 and 14b.

[0161] The figures show that yoghurt 16 prepared using strains Streptococcus thermophilus NIZO2274 and Lactobacillus delbrueckii subsp. bulgaricus NIZO2118 has a greater viscosity compared to the placebo yoghurt. These figures demonstrate that the amount and type of exopolysaccharides secreted by strains Streptococcus thermophilus NIZO2274 and Lactobacillus delbrueckii subsp. bulgaricus NIZO2118 produce a more viscous yoghurt.

Example 1BFinished Yoghurt Product

[0162] A yoghurt product was prepared in order to investigate the potential of using a composition according to the invention in a weight management yoghurt product. In particular, the formulation, blending and organoleptic properties were investigated.

[0163] Three EFSA (European Food Safety Authority) approved ingredients, GOS prebiotic, glucomannan and chromium were added to a yoghurt produced via a common yoghurt protocol as outlined above in Example 1A using a unique combination of strains of Streptococcus thermophilus NIZO2274=NCIMB 700859=Cell Deposit Ref: CBS 139100) and Lactobacillus delbrueckii subsp. bulgaricus (NIZO2118=Cell Deposit Ref: CBS 139099) in order to assess product acceptability via a panel (n=4). Different formulations were tested and two types were considered acceptable in terms of taste, texture/consistency and mouth feel.

[0164] In the recipe, the yoghurt was prepared separately using the 2 strains: Streptococcus thermophilus and Lactobaccillus bulgaricus and the combination of these 2 strains led to a high viscosity yoghurt (69 sec posthumus).

[0165] To the standard ingredients, GOS prebiotic, glucomannan and chromium were added via the fruit. A serving size of 150 g was used which contained per serving size 6 g of prebiotic, 1 g of glucomannan, 130 g chromium.

TABLE-US-00007 Formulation Standard GLU GLU/Chro/prebiotic g/100 g g/100 g g/100 g yoghurt 75 74 67 sucrose 5 5 5 chromiumchloride 1% 0, 0087 glucomannan 0, 67 0, 67 GOS 56% 7, 1 fruitpreparation 20 20 20

[0166] In these recipes, the same type of yoghurt was used. However, if desired, the protein and sucrose content of the yoghurt may be adjusted to optimise the protein and desired sweetness.

[0167] The yoghurt was prepared using the common yoghurt protocol. To the yoghurt a strawberry fruit preparation was added. In comparison to the standard recipe, GOS prebiotic, glucomannan and/or chromium were mixed with the strawberry fruit preparation with no issues. After mixing in the glucomannan, the strawberry fruit preparation became very viscous/thick within 30 min which was consequently used to prepare the fruited yoghurt in three ways after addition of the ingredients. [0168] Direct mixing of the fruit preparation and yoghurt to a homogeneous product; [0169] Directly adding the fruit preparation on the bottom to stiffen, before pouring the yoghurt on top; and [0170] Stiffening the strawberry preparation and gently mixing in the yoghurt to maintain pieces.

[0171] With reference to FIG. 6, three types of yoghurt were prepared and tested: (1) fruit on the bottom, (2) fruit mixed in as pieces and (3) fruit mixed in homogeneous. Upon a short organoleptic assessment of the product type by consumer type panel (n=4) the different preparations revealed different sensations: [0172] (Type 1) The key effect of the addition of the glucomannan to the fruit was that although the visual impression was that it was a tough gel, it was actually very smooth, viscous and easy to smoothen in the mouth. Yoghurt flavour was recognizable. [0173] (Type 2) The presence of fruit pieces (or on the bottom and mixed in with the yoghurt portion) provided a more fruity sensation of the product. Yoghurt flavour was recognizable. [0174] (Type 3) The homogeneous product was evaluated as gluey like/sticky and coating the mouth with a less attractive flavour.

[0175] All yoghurt types had a thickness which was expected for a stirred yoghurt type. The sweetness of the yoghurt types differed. The yoghurt type 1 was regarded as less sweet compared to type 3.

[0176] The combination of GOS, glucomannan, chromium in the levels tested could produce an acceptable product when used by addition via a fruit preparation. The samples containing ingredients in the fruit preparation and provided as fruit on the bottom and/or as pieces in the product (type 1 and type 2) were considered the best and acceptable in terms of taste, texture/consistency and mouth feel.

Example 2Worked Examples

[0177] The following yoghurt products are worked examples of yoghurts which may be produced in accordance with the present invention.

[0178] A yoghurt product may be produced in-line with that described with Example 1B Finished Yoghurt Product as detailed above. However, rather than adding all of the standard ingredients (GOS prebiotic, glucomannan and chromium) via the fruit, only two of these ingredients are added to the yoghurt (either: GOS prebiotic+glucomannan; GOS prebiotic+chromium; or glucomannan and chromium).

[0179] Formulations for the worked examples are follows:

TABLE-US-00008 Component g/100 g Yoghurt 75 Sucrose 5 Chromiumchloride 1% 0.010 GOS 56% 10 Fruit preparation 20

Formulation 2A

[0180]

TABLE-US-00009 Component g/100 g Yoghurt 75 Sucrose 5 Glucomannan 1.5 GOS 56% 9 Fruit preparation 20

Formulation 2B

[0181]

TABLE-US-00010 Component g/100 g Yoghurt 75 Sucrose 5 Chromiumchloride 1% 0.0075 Glucomannan 1 Fruit preparation 20

Formulation 2C

[0182] As with Example 1B, if desired, the protein and sucrose content of the yoghurt may be adjusted to optimise the protein and desired sweetness.

[0183] The forgoing embodiments are not intended to limit the scope of the protection afforded by the claims, but rather to describe examples of how the invention may be put into practice.

Indications of Deposited Biological Material

[0184] A deposition of biological material by NIZO Food Research BV, of Kernhemseweg 2, 6718 ZB EDE, The Netherlands, was made at Centraalbureau voor Schimmelcultures, Uppsalalaan 8, P.O. Box 85167, 3508 AD UTRECHT, The Netherlands for the purposes of filing one or more patent applications. The Centraalbureau voor Schimmelcultures is a recognised International Depository Authority (IDA) under the Budapest Treaty and the deposition of biological material was made on the same terms as those laid down in the Treaty. Each of the strains deposited at the Centraalbureau voor Schimmelcultures have been assigned a number along with the prefix CBS.

[0185] NIZO Food Research BV has authorised the Applicant to refer to the deposited biological material in this patent application and has provided their unreserved and irrevocable consent to the deposited material being made available to the public as from the date of filing of the patent application, including any subsequently filed patent application claiming priority therefrom.

[0186] The deposited biological referred to in this application is as follows: [0187] Name: Centraalbureau voor Schimmelcultures [0188] Address: Uppsalalaan 8 [0189] P.O. Box 85167 [0190] 3508 AD UTRECHT [0191] The Netherlands [0192] Date: 25 Nov. 2014 [0193] Accession Number: CBS 139099 [0194] Description: Lactobacillus delbrueckii subsp.bulgaricus [0195] and [0196] Name: Centraalbureau voor Schimmelcultures [0197] Address: Uppsalalaan 8 [0198] P.O. Box 85167 [0199] 3508 AD UTRECHT [0200] The Netherlands [0201] Date: 25 Nov. 2014 [0202] Accession Number: CBS 139100 [0203] Description: Streptococcus thermophilus

LIST OF REFERENCES

[0204] Abdollahi M, Farshchi A, Nikfar S, Seyedifar M. Effect of chromium on glucose and lipid profiles in patients with type 2 diabetes; a meta-analysis review of randomized trials. J Pharm Pharm Sci. 2013; 16(1):99-114. [0205] ABRAMS, S. A., GRIFFIN, I. J., HAWTHORNE, K. M. & ELLIS, K. J. 2007. Effect of prebiotic supplementation and calcium intake on body mass index. J Pediatr, 151, 293-8. [0206] ACHANTA, K., ARYANA, K. J., BOENEKE, C. A. 2007. Fat free plain set yogurts fortified with various minerals. Food Sci Tech, 40, 424-429. [0207] ANDERSON, R. A. 1998a. Chromium, glucose intolerance and diabetes. J Am Coll Nutr, 17, 548-55. [0208] ANDERSON, R. A. 1998b. Effects of chromium on body composition and weight loss. Nutr Rev, 56, 266-70. [0209] ARCHER, B. J., JOHNSON, S. K., DEVEREUX, H. M. & BAXTER, A. L. 2004. Effect of fat replacement by inulin or lupin-kernel fibre on sausage patty acceptability, post-meal perceptions of satiety and food intake in men. Br J Nutr, 91, 591-9. [0210] ATTENBURROW, M. J., ODONTIADIS, J., MURRAY, B. J., COWEN, P. J. & FRANKLIN, M. 2002. Chromium treatment decreases the sensitivity of 5-HT2A receptors. Psychopharmacology (Berl), 159, 432-6. [0211] BATES B, LENNOX A, BATES C et al. (2011) National Diet and Nutrition Survey. Headline results from years 1 and 2 (combined) of the Rolling Programme (2008/2009-2009/10). Food Standards Agency & Department of Health, London. [0212] BOHM, A., KAISER, I., TREBSTEIN, A. & HENLE, T. 2005. Heat-induced degradation of inulin. European Food Research and Technology, 220, 466-471. [0213] CANI, P. D., JOLY, E., HORSMANS, Y. & DELZENNE, N. M. 2006. Oligofructose promotes satiety in healthy human: a pilot study. Eur J Clin Nutr, 60, 567-72. [0214] CANI, P. D., HOSTE, S., GUIOT, Y. & DELZENNE, N. M. 2007. Dietary non-digestible carbohydrates promote L-cell differentiation in the proximal colon of rats. Br J Nutr, 98, 32-7. [0215] CANI, P. D., LECOURT, E., DEWULF, E. M., SOHET, F. M., PACHIKIAN, B. D., NASLAIN, D., DE BACKER, F., NEYRINCK, A. M. & DELZENNE, N. M. 2009. Gut microbiota fermentation of prebiotics increases satietogenic and incretin gut peptide production with consequences for appetite sensation and glucose response after a meal. Am J Clin Nutr, 90, 1236-43. [0216] CARABIN, I. G. & FLAMM, W. G. 1999. Evaluation of safety of inulin and oligofructose as dietary fiber. Regul Toxicol Pharmacol, 30, 268-82. [0217] CHEN, H. L., CHENG, H. C., LIU, Y. J., LIU, S. Y. & WU, W. T. 2006. Konjac acts as a natural laxative by increasing stool bulk and improving colonic ecology in healthy adults. Nutrition, 22, 1112-9. [0218] CHEN, H. L., CHENG, H. C., WU, W. T., LIU, Y. J. & LIU, S. Y. 2008. Supplementation of konjac glucomannan into a low-fiber Chinese diet promoted bowel movement and improved colonic ecology in constipated adults: a placebo-controlled, diet-controlled trial. J Am Coll Nutr, 27, 102-8. [0219] CHUA, M., BALDWIN, T. C., HOCKING, T. J. & CHAN, K. 2010. Traditional uses and potential health benefits of Amorphophallus konjac K. Koch ex N. E. Br. J Ethnopharmacol, 128, 268-78. [0220] DELZENNE, N. M., CANI, P. D., DAUBIOUL, C. & NEYRINCK, A. M. 2005. Impact of inulin and oligofructose on gastrointestinal peptides. Br J Nutr, 93 Suppl 1, S157-61. [0221] DEVEREUX, H. M., JONES, G. P., McCORMACK, L., & HUNTER, W. C. 2003. Consumer acceptability of low fat foods containing inulin and oligofructose. J Food Sc, 68, 1850-1854. [0222] DEPARTMENT OF HEALTH (1991): Dietary Reference Values for Food Energy and Nutrients for the United Kingdom. Report on Health and Social Subjects No. 41. HSMO, London [0223] DOI, K. 1995. Effect of konjac fibre (glucomannan) on glucose and lipids. Eur J Clin Nutr, 49 Suppl 3, S190-7. [0224] GENTA, S., CABRERA, W., HABIB, N., PONS, J., CARILLO, I. M., GRAU, A. & SANCHEZ, S. 2009. Yacon syrup: beneficial effects on obesity and insulin resistance in humans. Clin Nutr, 28, 182-7. [0225] HABIB, A. M., RICHARDS, P., ROGERS, G. J., REIMANN, F. & GRIBBLE, F. M. 2013. Co-localisation and secretion of glucagon-like peptide 1 and peptide YY from primary cultured human L cells. Diabetologia, 56, 1413-6. [0226] HARROLD, J. A., HUGHES, G. M., O'SHIEL, K., QUINN, E., BOYLAND, E. J., WILLIAMS, N. J. & HALFORD, J. C. 2013. Acute effects of a herb extract formulation and inulin fibre on appetite, energy intake and food choice. Appetite, 62, 84-90. [0227] HESS, J. R., BIRKETT, A. M., THOMAS, W. & SLAVIN, J. L. 2011. Effects of short-chain fructooligosaccharides on satiety responses in healthy men and women. Appetite, 56, 128-34. [0228] Hoeger W W, Harris C, Long E M, Hopkins DR. Four-week supplementation with a natural dietary compound produces favorable changes in body composition. Adv. Ther. 1998 September-October; 15 (5):305-14. [0229] HUEBNER, J., WEHLING, R. L., PARKHURST, A. & HUTKINS, R. W. 2008. Effect of processing conditions on the prebiotic activity of commercial prebiotics. International Dairy Journal, 18, 287-293. [0230] KROL, E., KREJPCIO, Z., BYKS, H., BOGDANSKI, P. & PUPEK-MUSIALIK, D. 2011. Effects of chromium brewer's yeast supplementation on body mass, blood carbohydrates, and lipids and minerals in type 2 diabetic patients. Biol Trace Elem Res, 143, 726-37. [0231] MARTINO, F., PUDDU, P. E., PANNARALE, G., COLANTONI, C., MARTINO, E., NIGLIO, T., ZANONI, C. & BARILLA, F. 2013. Low dose chromium-polynicotinate or policosanol is effective in hypercholesterolemic children only in combination with glucomannan. Atherosclerosis, 228, 198-202. [0232] MAYNARD, L. M., WISEMANDLE, W., ROCHE, A. F., CHUMLEA, W. C., GUO, S. S. & SIERVOGEL, R. M. 2001. Childhood body composition in relation to body mass index. Pediatrics, 107, 344-50. [0233] NIHON BIORESEARCH INC. HASHIMA LABORATORY, JAPAN. 1992. Study No. 823 1, Antigenicity Study of Propal A Using Bacteria [0234] OKETANI Y, ICHIKAWA K, ONO, C. 1991. Toxicity studies on glucomannan (1) acute toxicity in mice and rats. Journal of Applied Pharmacology, 27, 127-131. [0235] ONAKPOYA, I., POSADZKI, P. & ERNST, E. 2013. Chromium supplementation in overweight and obesity: a systematic review and meta-analysis of randomized clinical trials. Obes Rev, 14, 496-507. [0236] OVERDUIN, J., FRAYO, R. S., GRILL, H. J., KAPLAN, J. M. & CUMMINGS, D. E. 2005. Role of the duodenum and macronutrient type in ghrelin regulation. Endocrinology, 146, 845-50. [0237] PARNELL, J. A. & REIMER, R. A. 2009. Weight loss during oligofructose supplementation is associated with decreased ghrelin and increased peptide YY in overweight and obese adults. Am J Clin Nutr, 89, 1751-9. [0238] PETERS, H. P., BOERS, H. M., HADDEMAN, E., MELNIKOV, S. M. & QVYJT, F. 2009. No effect of added beta-glucan or of fructooligosaccharide on appetite or energy intake. Am J Clin Nutr, 89, 58-63. [0239] PITTLER, M. H., STEVINSON, C. & ERNST, E. 2003. Chromium picolinate for reducing body weight: meta-analysis of randomized trials. Int J Obes Relat Metab Disord, 27, 522-9. [0240] SALAS-SALVADO, J., FARRES, X., LUQUE, X., NAREJOS, S., BORRELL, M., BASORA, J., ANGUERA, A., TORRES, F., BULLO, M. & BALANZA, R. 2008. Effect of two doses of a mixture of soluble fibres on body weight and metabolic variables in overweight or obese patients: a randomised trial. Br J Nutr, 99, 1380-7. [0241] SOOD, N., BAKER, W. L. & COLEMAN, C. I. 2008. Effect of glucomannan on plasma lipid and glucose concentrations, body weight, and blood pressure: systematic review and meta-analysis. Am J Clin Nutr, 88, 1167-75. [0242] TARINI, J. & WOLEVER, T. M. 2010. The fermentable fibre inulin increases postprandial serum short-chain fatty acids and reduces free-fatty acids and ghrelin in healthy subjects. Appl Physiol Nutr Metab, 35, 9-16. [0243] VERHOEF, S. P., MEYER, D. & WESTERTERP, K. R. 2011. Effects of oligofructose on appetite profile, glucagon-like peptide 1 and peptide YY3-36 concentrations and energy intake. Br J Nutr, 106, 1757-62. [0244] VUKSAN, V., JENKINS, A. L., ROGOVIK, A. L., FAIRGRIEVE, C. D., JOVANOVSKI, E. & LEITER, L. A. 2011. Viscosity rather than quantity of dietary fibre predicts cholesterol-lowering effect in healthy individuals. Br J Nutr, 106, 1349-52. [0245] VUKSAN, V., JENKINS, D. J., SPADAFORA, P., SIEVENPIPER, J. L., OWEN, R., VIDGEN, E., BRIGHENTI, F., JOSSE, R., LEITER, L. A. & BRUCE-THOMPSON, C. 1999. Konjac-mannan (glucomannan) improves glycemia and other associated risk factors for coronary heart disease in type 2 diabetes. A randomized controlled metabolic trial. Diabetes Care, 22, 913-9. [0246] VUKSAN, V., SIEVENPIPER, J. L., OWEN, R., SWILLEY, J. A., SPADAFORA, P., JENKINS, D. J., VIDGEN, E., BRIGHENTI, F., JOSSE, R. G., LEITER, L. A., XU, Z. & NOVOKMET, R. 2000. Beneficial effects of viscous dietary fiber from Konjac-mannan in subjects with the insulin resistance syndrome: results of a controlled metabolic trial. Diabetes Care, 23, 9-14. [0247] YAZAKI, Y., FARIDI, Z., MA, Y., ALI, A., NORTHRUP, V., NJIKE, V. Y., LIBERTI, L. & KATZ, D. L. 2010. A pilot study of chromium picolinate for weight loss. J Altern Complement Med, 16, 291-9. [0248] YOSHIDA, M., VANSTONE, C. A., PARSONS, W. D., ZAWISTOWSKI, J. & JONES, P. J. 2006. Effect of plant sterols and glucomannan on lipids in individuals with and without type II diabetes. Eur J Clin Nutr, 60, 529-37