Sample extraction support tool and sample extraction method

Abstract

The sample extraction support tool is placed on an extraction container having at least one well for accommodating a sample extracted from a test piece holding the sample in a microchannel provided therein with both ends open. The sample extraction support tool includes at least one test piece holding portion having an opening for guiding a sample extracted from the test piece to a bottom of the well and configured to hold the test piece at a position where the test piece is not in contact with the sample which is extracted from the test piece and is stored on the bottom of the well, and in a state where one end of the microchannel is directed to the bottom of the well.

Claims

1. A sample extraction tool comprising a test piece and a sample extraction support tool, wherein the test piece has a longitudinal direction and includes a microchannel for holding a sample, which is parallel to the longitudinal direction of the test piece, of which both ends open, the sample extraction support tool is used while being placed on an extraction container having at least one well for accommodating the sample extracted from the test piece, and the sample extraction support tool comprises at least one test piece holding portion, the test piece holding portion has an opening for guiding the sample extracted from the test piece to a bottom of the well, and is configured to hold the test piece at a position where the test piece is not in contact with the sample which is extracted from the test piece and is stored on the bottom of the well, and in a state where one end of the microchannel is directed to the bottom surface of the well.

2. The sample extraction support tool according to claim 1, wherein the opening is provided at a lower end of the test piece holding portion and has a size not allow the test piece to pass through, and at least part of an inner surface of the test piece holding portion is inclined so as to guide the sample extracted from the test piece to the opening.

3. A sample extraction support tool which is used while being placed on an extraction container having at least one well for accommodating a sample extracted from a test piece holding the sample in a microchannel provided therein with both ends open, wherein the sample extraction support tool comprises at least one test piece holding portion, the test piece holding portion has an opening for guiding the sample extracted from the test piece to a bottom of the well, and is configured to hold the test piece at a position where the test piece is not in contact with the sample which is extracted from the test piece and is stored on the bottom of the well, and in a state where one end of the microchannel is directed to the bottom surface of the well, and wherein the extraction container is a well-plate in which a plurality of the wells are provided on a same plane, and the sample extraction support tool is a plate-shaped member having the test piece holding portions corresponding to each of the wells of the extraction container.

4. A sample extraction method for extracting a sample from a test piece, wherein the test piece has a longitudinal direction and includes a microchannel for holding a sample, which is parallel to the longitudinal direction of the test piece, of which both ends open, the sample extraction method comprises: a support tool placing step of placing, on an extraction container having at least one well for accommodating a sample extracted from the test piece, a sample extraction support tool including at least one test piece holding portion, the test piece holding portion having an opening for guiding a sample extracted from the test piece to a bottom of the well and configured to hold the test piece at a position where the test piece is not in contact with a sample which is extracted from the test piece and stored on the bottom of the well, and in a state where one end of the microchannel is directed to the bottom of the well; a piece setting step of causing the test piece holding portion of the sample extraction support tool to hold the test piece; and a sample extracting step of extracting a sample from the test piece to the bottom of the well by swiveling the extraction container so that a centrifugal force acts in a direction from a top side of the well to a bottom side of the well of the extraction container, in this order.

5. The sample extraction method according to claim 4, further comprising: a test piece removing step of removing the test piece from the well of the extraction container by moving the sample extraction support tool from above the extraction container after the sample extracting step.

6. The sample extraction method according to claim 4, wherein the opening of the sample extraction support tool is provided at a lower end of the test piece holding portion and has a size not allow the test piece to pass through, and at least part of an inner surface of the test piece holding portion is inclined so as to guide the sample extracted from the test piece to the opening.

7. A sample extraction method comprising: a support tool placing step of placing, on an extraction container having at least one well for accommodating a sample extracted from a test piece holding the sample in a microchannel provided therein with both ends open, a sample extraction support tool including at least one test piece holding portion, the test piece holding portion having an opening for guiding a sample extracted from the test piece to a bottom of the well and configured to hold the test piece at a position where the test piece is not in contact with a sample which is extracted from the test piece and stored on the bottom of the well, and in a state where one end of the microchannel is directed to the bottom of the well; a piece setting step of causing the test piece holding portion of the sample extraction support tool to hold the test piece; and a sample extracting step of extracting a sample from the test piece to the bottom of the well by swiveling the extraction container so that a centrifugal force acts in a direction from a top side of the well to a bottom side of the well of the extraction container, in this order, wherein the extraction container is a well-plate in which a plurality of the wells are provided on a same plane, and the sample extraction support tool is a plate-shaped member having the test piece holding portions corresponding to each of the wells of the extraction container.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) FIG. 1 is a cross-sectional view showing an embodiment of a sample extraction support tool together with an extraction container.

(2) FIG. 2 is a cross-sectional view showing a sample extraction process using the sample extraction support tool of the embodiment in order.

(3) FIG. 3 is a flowchart showing a sample extraction method using the sample extraction support tool of the embodiment.

EMBODIMENT OF THE INVENTION

(4) Hereinafter, an embodiment of the sample extraction support tool and an embodiment of the sample extraction method using the sample extraction support tool will be described with reference to the drawings.

(5) As shown in FIG. 1, a sample extraction support tool 2 of the present embodiment is used together with an extraction container 10 having a plurality of wells 12 having an open upper part. The sample extraction support tool 2 is a member on a plate having a test piece holding portion 4 corresponding to each of the wells 12 of the extraction container 10. The test piece holding portion 4 has a funnel shape in which the inner diameter decreases toward the bottom, and an opening 6 is provided at the lower end. Note that the shape of the test piece holding portion 4 is not limited to the funnel shape, and may be one in which at least part of the inner surface is inclined so that a sample extracted from a test piece 20 is guided to the opening 6 at the lower end.

(6) As shown in FIG. 2(A), the sample extraction support tool 2 is used in a state of being placed on the extraction container 10. The test piece 20 holding the sample to be extracted is inserted into the test piece holding portion 4 from above. The inner diameter of the opening 6 of the test piece holding portion 4 has size that prevents the test piece 20 from falling to the bottom of the well 12. The test piece 20 inserted into the test piece holding portion 4 is supported by the inner side surface of the test piece holding portion 4 in a state where one end is directed to the bottom of the well 12.

(7) The test piece 20 has a microchannel with both ends open therein, and holds a sample in the microchannel. The test piece 20 may be a piece cut from a micro-sampling device as disclosed in Patent Document 1, or may have a capillary shape such as a capillary.

(8) In a state where the test piece 20 is held in each of the test piece holding portions 4 of the sample extraction support tool 2, an integral body of the sample extraction support tool and the extraction container 10 is swiveled so that a centrifugal force acts in the direction from a top side of the well 12 to a bottom side of the well 12, so that, as shown in FIG. 2(B), a sample flows out from one end of the test piece 20 and the sample is extracted to the bottom of the well 12 through the opening 6 of the test piece holding portion 4. At this time, since the test piece 20 is held by the test piece holding portion 4 at a position where the test piece 20 is not in contact with the sample extracted on the bottom of the well 12, the extracted sample is not sucked again into the test piece 20.

(9) After that, as shown in FIG. 2(C), all the test pieces 20 can be removed from the wells 12 as the sample extraction support tool 2 is moved from above the extraction container 10. For this reason, processing on the sample in the wells 12 can be facilitated.

(10) That is, as shown in FIG. 3, the sample extraction method using the sample extraction support tool 2 of the present embodiment includes four steps described below.

(11) (Step S1) Place the sample extraction support tool 2 on the extraction container 10.

(12) (Step S2) Cause each of the test piece holding portions 4 of the sample extraction support tool 2 to hold the test piece 20.

(13) (Step S3) Swivel the extraction container 10 with the sample extraction support tool 2 placed on to extract the sample using a centrifugal force.

(14) (Step S4) Move the sample extraction support tool 2 from above the extraction container 10.

(15) Note that the sample extraction support tool 2 of the above embodiment is applied to the extraction container 10 having a plurality of the wells 12. However, the present invention is not limited to this, and can be similarly applied to an extraction container having only one well.

DESCRIPTION OF REFERENCE SIGNS

(16) 2: Sample extraction support tool 4: Test piece holding portion 6: Opening 10: Extraction container 12: Well 20: Test piece 22: Microchannel