AXIAL DISPERSION BIOREACTOR (ADBR) FOR PRODUCTION OF MICROALGAE AND OTHER MICROORGANISMS

Abstract

An Axial Dispersion Bioreactor (ADBR) is designed for the photoautotrophic, mixotrophic or heterotrophic growth and production of microalgae and other microorganisms (bacteria, fungi, etc.) as well as cell cultures of plants, animals, insects and others. The ADBR is equipped with a plate having a plurality of holes that moves longitudinally or axially within the bioreactor to effect superior hydrodynamic and mixing patterns. The ADBR has the advantages of providing a low-shear culture environment, superior liquid mixing, and efficient gas mass transfer.

Claims

1. A bioreactor system comprising: a. a bioreactor vessel (100) for containing a fluid; b. at least one mixer (130) comprising a surface having a plurality of holes (140) disposed through the surface; and c. a mechanism (160) for moving the at least one mixer within the bioreactor vessel, wherein the movement of the at least one mixer enables mixing of the fluid in the bioreactor vessel to generate a hydrodynamic flow pattern.

2. (canceled)

3. The system of claim 1, wherein the bioreactor vessel is an axial dispersion bioreactor tank.

4. (canceled)

5. The system of claim 1, wherein the surface of the at least one mixer comprises a flat or curved surface.

6. (canceled)

7. A bioreactor system for the cultivation of biological cultures, the system comprising: a. a bioreactor vessel (100) for containing a liquid growth medium, comprising a bottom (110) and one or more sidewalls (120); b. one or more mixing plates (130), each comprising a plurality of through holes (140); and c. a mechanism (160) for moving the one or more mixing plates longitudinally or axially within the bioreactor vessel; wherein the movement of the one or more mixing plates enables mixing of the liquid growth medium and generation of a hydrodynamic flow pattern.

8.-11. (canceled)

12. The system of claim 7, wherein the one or more mixing plates each comprise a shape, wherein the shape is a circle, oval, ellipse, square, rectangle, parallelogram, quadrilateral, triangle, pentagon, hexagon, heptagon, octagon, polygon, or irregular shape.

13. The system of claim 7, wherein the one or more mixing plates each comprise a surface which is flat, rippled, textured, curved, concave, convex, zig-zag patterned, or has compound curves.

14. (canceled)

15. The system of claim 7, wherein the plurality of through holes each comprise a shape and a size, and wherein the shapes and sizes of the holes are either uniform or varied, wherein the shapes and sizes of the holes are configured to modify or enhance the hydrodynamic flow pattern or mixing of the liquid growth medium.

16. (canceled)

17. The system of claim 7, wherein the bioreactor comprises a plurality of rigid or flexible ribbons having two ends, wherein the ribbons are attached to the mixing plates at one or both ends, wherein the rigid or flexible ribbons are configured to modify or enhance the hydrodynamic flow pattern or mixing of the liquid growth medium.

18. (canceled)

19. (canceled)

20. The system of claim 7, wherein the movement of the one or more mixing plates is continuous or intermittent back-and-forth axial displacement at a regulated variable rate.

21. The system of claim 7, wherein one or more gasses are sparged or bubbled through the liquid growth medium from the bottom of the bioreactor with a specified bubble size and a gas flow rate for each gas.

22. (canceled)

23. The system of claim 7, wherein the mechanism for the movement of the one or more mixing plates comprises one of the following mechanisms: i. a piston rod and crankshaft which connect the one or more mixing plates with an external motor, and wherein the piston rod moves the one or more mixing plates longitudinally or axially within the vessel; ii. one or more threaded rods which pass through one or more threaded through holes in the one or more mixing plates and are attached to one or more external motors; iii. one or more pulleys which are turned by one or more external motors, wherein one or more belts are fixed to the one or more mixing plates, pass through the one or more pulleys, and move the one or more mixing plates longitudinally or axially within the vessel; or iv. two or more internal magnets attached to the one or more mixing plates and two or more movable exterior magnets on the other face of the one or more sidewalls, wherein movement of the exterior magnets moves the one or more mixing plates longitudinally or axially within the vessel.

24.-26. (canceled)

27. A method of promoting the growth and production of a biological culture contained in a bioreactor, the method comprising: a. providing a bioreactor, the bioreactor comprising: i. a bioreactor vessel (100), comprising a bottom (110) and one or more sidewalls (120); ii. one or more mixing plates (130), each comprising a plurality of through holes (140); and iii. a mechanism (160) for moving the one or more mixing plates longitudinally or axially within the bioreactor vessel; b. providing a biological culture, wherein the biological culture is dispersed within a liquid growth medium; c. adding the biological culture and liquid growth medium into the bioreactor; and d. moving the one or more mixing plates longitudinally or axially within the bioreactor vessel via the mechanism; wherein the movement of the one or more mixing plates mixes the liquid growth medium and generates a hydrodynamic flow pattern.

28.-31. (canceled)

32. The method of claim 27, wherein the one or more mixing plates each comprise a shape, wherein the shape is a circle, oval, ellipse, square, rectangle, parallelogram, quadrilateral, triangle, pentagon, hexagon, heptagon, octagon, polygon, or irregular shape.

33. The method of claim 27, wherein the one or more mixing plates each comprise a surface which is flat, rippled, textured, curved, concave, convex, zig-zag patterned, or has compound curves.

34. (canceled)

35. The method of claim 27, wherein the plurality of through holes each comprise a shape and a size, and wherein the shapes and sizes of the holes are either uniform or varied, wherein the shapes and sizes of the holes are configured to modify or enhance the hydrodynamic flow pattern or mixing of the liquid growth medium.

36. (canceled)

37. The method of claim 27, wherein the bioreactor comprises a plurality of rigid or flexible ribbons having two ends, wherein the ribbons are attached to the mixing plates at one or both ends, wherein the rigid or flexible ribbons are configured to modify or enhance the hydrodynamic flow pattern or mixing of the liquid growth medium.

38. (canceled)

39. The method of claim 27, wherein the one or more mixing plates have a spacing which is configured to modify or enhance the hydrodynamic flow pattern or mixing of the liquid growth medium.

40. The method of claim 27, wherein the movement of the one or more mixing plates is continuous or intermittent back-and-forth axial displacement at a regulated variable rate.

41. The method of claim 27, wherein one or more gasses are sparged or bubbled through the liquid growth medium from the bottom of the bioreactor with a specified bubble size and a gas flow rate for each gas.

42. (canceled)

43. The method of claim 27, wherein the mechanism for the movement of the one or more mixing plates comprises one of the following mechanisms: i. a piston rod and crankshaft which connect the one or more mixing plates with an external motor, and wherein the piston rod moves the one or more mixing plates longitudinally or axially within the vessel; ii. one or more threaded rods which pass through one or more threaded through holes in the one or more mixing plates and are attached to one or more external motors; iii. one or more pulleys which are turned by one or more external motors, wherein one or more belts are fixed to the one or more mixing plates, pass through the one or more pulleys, and move the one or more mixing plates longitudinally or axially within the vessel; or iv. two or more internal magnets attached to the one or more mixing plates and two or more movable exterior magnets on the other face of the one or more sidewalls, wherein movement of the exterior magnets moves the one or more mixing plates longitudinally or axially within the vessel.

44.-46. (canceled)

Description

BRIEF DESCRIPTION OF THE SEVERAL VIEWS OF THE DRAWING(S)

[0016] The features and advantages of the present invention will become apparent from a consideration of the following detailed description presented in connection with the accompanying drawings in which:

[0017] FIG. 1 shows a non-limiting schematic of a cylindrical Axial Dispersion Bioreactor (ADBR) with a perforated plate that moves back and forth along the axial or longitudinal axis of the bioreactor, effecting a hydrodynamic flow pattern and mixing as the liquid medium is forced through the plate holes as the plate moves in either direction.

[0018] FIG. 2 shows a non-limiting schematic of an ADBR with a rectangular perforated plate that moves back and forth along the axial or longitudinal axis of the bioreactor.

[0019] FIG. 3 shows a non-limiting schematic of an ADBR with a cylindrical plate, where the threaded rod rotates in either radial direction to move the plate upward or downward along the axial direction of the bioreactor. The guide rods prevent the plate from spinning or rotating as the threaded rod rotates.

[0020] FIG. 4 shows a non-limiting schematic of an ADBR with a rectangular plate, where the threaded rod rotates in either radial direction to move the plate upward or downward along the axial direction of the bioreactor. The right-angled corners of the bioreactor prevent the plate from spinning or rotating as the threaded rod rotates.

[0021] FIG. 5 shows a non-limiting schematic of an ADBR with a rectangular plate, where the pulley systems rotate in either direction to move the plate upward or downward along the axial direction of the bioreactor.

[0022] FIG. 6A shows a non-limiting schematic of an ADBR with a rectangular plate equipped with magnetic rods at opposite ends of the plate, where the upward or downward movement of corresponding external magnets move the plate accordingly along the axial direction of the bioreactor.

[0023] FIG. 6B shows a non-limiting schematic of the ADBR shown in FIG. 6A with the addition of a guide rod.

[0024] FIG. 7 shows a non-limiting schematic of single and double/multiple ADBR plates assuming circular and flat-surface geometric configurations.

[0025] FIG. 8 shows a non-limiting schematic of double/multiple ADBR plates assuming rectangular and flat-surface geometric configurations.

[0026] FIG. 9 shows side views of non-limiting embodiments of single and double/multiple ADBR plates assuming curved-surface configurations, and which may be rectangular, square, circular, elliptical, etc., in top-view geometry.

[0027] FIG. 10 shows side views of non-limiting embodiments of single and multiple ADBR plates assuming various surface configurations as shown, and which may be rectangular, square, circular, elliptical, etc., in top-view geometry.

[0028] FIGS. 11A and 11B show non-limiting embodiments of rigid or flexible ribbons attached to the ADBR plate in various patterns to modify or enhance the resulting hydrodynamic patterns or mixing of the liquid medium.

[0029] FIG. 12 shows non-limiting embodiments of the ADBR plate with varying hole shapes, sizes and patterns.

[0030] FIG. 13 shows non-limiting embodiments of the ADBR plate with varying hole shapes, sizes and patterns.

[0031] FIG. 14 shows non-limiting embodiments of the ADBR plate with varying hole shapes, sizes and patterns.

[0032] FIG. 15 shows non-limiting embodiments of the ADBR plate with varying hole shapes, sizes and patterns.

[0033] FIG. 16 shows another non-limiting example of the ABDR system.

DESCRIPTION OF PREFERRED EMBODIMENTS

[0034] Following is a list of elements corresponding to a particular element referred to herein: [0035] 100 Bioreactor [0036] 110 Bottom [0037] 120 Sidewall [0038] 130 Mixing plate [0039] 140 Through hole [0040] 150 Guide rod (or threaded rod) [0041] 160 Mechanism for movement

[0042] As used herein, the terms axial and longitudinal are interchangeable. When used in the context of movement, axial or longitudinal movement refers to linear displacement or translation of the plates about a linear path. For example, the plates may move in an up and down motion about an axis within the bioreactor vessel. As another example, in one of the embodiments with the threaded axis, the mechanism of effecting the axial displacement of the plate is by rotating the threaded axial rod. Since the plate may be constrained in place by the walls of bioreactor, the plate then moves linearly along the axis. Reversing the rotation of the threaded axial rod also reverses the direction of the linear displacement of the plate.

[0043] As used herein, the terms radial and cross-sectional are also synonymous, and are perpendicular to the axial and longitudinal direction.

[0044] Alternatively or in combination, in some embodiments, movements of the ADBR plate may further comprise i) rotating around an axis, ii) moving up and down (vertically) while rotating, and/or iii) moving sideways-horizontally, while optionally rotating and/or moving up and down.

[0045] As used herein, hydrodynamic refers to liquid mixing and hydrodynamic flow pattern refers to the resultant pattern of liquid mixing as caused by the movement of the plates.

[0046] Referring to FIGS. 1-16, in one embodiment, the present invention features an axial dispersion bioreactor for the cultivation of biological cultures. As a non-limiting example, the bioreactor may comprise: a bioreactor vessel (100) comprising a bottom (110) and one or more sidewalls (120); one or more mixing plates (130), each comprising a plurality of through holes (140); and a mechanism (160) for moving the one or more mixing plates (130) longitudinally or axially within the bioreactor vessel (100). Without wishing to limit the invention to a particular theory or mechanism, the movement of the one or more mixing plates enables the mixing of a liquid growth medium and the generation of a hydrodynamic flow pattern.

[0047] In another embodiment, the present invention features a method of promoting the growth and production of a biological culture contained in a bioreactor. As a non-limiting example, the method may comprise: (a) providing a bioreactor, the bioreactor comprising: (i) a bioreactor vessel (100) comprising a bottom (110) and one or more sidewalls (120); (ii) one or more mixing plates (130), each comprising a plurality of through holes (140), wherein the one or more mixing plates (130) are configured to move longitudinally or axially within the bioreactor vessel (100); and (iii) a mechanism (160) for moving the one or more mixing plates (130) longitudinally or axially within the bioreactor vessel (100); (b) providing a biological culture, wherein the biological culture is dispersed within a liquid growth medium; (c) adding the biological culture and liquid growth medium into the bioreactor vessel (100); and (d) moving the one or more mixing plates (130) longitudinally or axially within the bioreactor vessel (100) via the mechanism (160). Preferably, the movement of the one or more mixing plates mixes the liquid growth medium and generates a hydrodynamic flow pattern.

[0048] In some embodiments, the bioreactor (a non-limiting example shown in FIG. 16) may be used for photoautotrophic, mixotrophic or heterotrophic growth of a biological culture. In further embodiments, the bioreactor may be used for the growth and production of microalgae, bacteria, fungi, or other microorganisms, as well as cell cultures of plants, animals, insects and others. In still further embodiments, the movement of the one or more mixing plates may be configured to provide a low-shear culture environment, superior liquid mixing, and efficient gas mass transfer.

[0049] According to one embodiment, the bottom of the vessel may comprise a shape which gives the vessel a cross-sectional shape, and the one or more mixing plates may comprise a same or different shape. Non-limiting examples of the shapes of the bottom, cross-section, and mixing plates include: a circle, oval, ellipse, square, rectangle, parallelogram, quadrilateral, triangle, pentagon, hexagon, heptagon, octagon, polygon, or irregular shape. In another embodiment, as shown in FIGS. 9-10, the one or more mixing plates may each comprise a surface which is flat, rippled, textured, curved, concaved, convexed, zig-zag patterned, or having compound curves.

[0050] In some other embodiments, the plurality of through holes may each comprise a shape and a size. Referring to FIGS. 12-15, the shapes and sizes of the through holes may be uniform or varied. In yet other embodiments, as shown in FIGS. 11A-11B, the bioreactor may comprise a plurality of rigid or flexible ribbons having two ends, wherein the ribbons are attached to the mixing plates at one or both ends. In still other embodiments, the one or more mixing plates may have a spacing between the mixing plates. In preferred embodiments, any or all of (1) the shapes and sizes of the through holes, (2) the density or number per unit area of the through holes, (3) the rigid or flexible ribbons, and (4) the spacing between the one or more mixing plates, may be configured to modify or enhance the hydrodynamic flow pattern or mixing of the liquid growth medium.

[0051] In one embodiment, the movement of the one or more mixing plates may be continuous or intermittent back-and-forth axial displacement at a regulated variable rate. In some embodiments, the mixing plates may move back-and-forth in a vertical direction. In other embodiments, the mixing plates may move back-and-forth in a horizontal direction. In another embodiment, one or more gasses are sparged or bubbled through the liquid growth medium from the bottom of the bioreactor with a specified bubble size and a gas flow rate for each gas. Non-limiting example gasses include oxygen, nitrogen, carbon dioxide and carbon monoxide. In yet another embodiment, the one or more sidewalls of the bioreactor may be either transparent or non-transparent. Without wishing to limit the present invention to any particular theory or mechanism, the transparent sidewalls are believed to be advantageous for photoautotrophic and mixotrophic production.

[0052] In some embodiments, the mechanism for the movement of the one or more mixing plates may comprise a piston rod and crankshaft which connect the one or more mixing plates with an external motor, and wherein the piston rod moves the one or more mixing plates longitudinally or axially within the vessel. As shown in FIGS. 3-4, in other embodiments, the mechanism for the movement of the one or more mixing plates may comprise one or more threaded rods which pass through one or more threaded through holes in the one or more mixing plates and are attached to one or more external motors. In further embodiments, rotation of the one or more mixing plates may be prevented by the shape of the one or more mixing plates and the shape of the cross-section of the vessel, or by use of one or more guide rods (150) configured to fit through one or more holes in the one or more mixing plates. For example, the mechanism of effecting the axial displacement of the plate is by rotating the threaded axial rod. As the plate is constrained in place by the walls of bioreactor, the plate then moves linearly along the axis. Reversing the rotation of the threaded axial rod also reverses the direction of the linear displacement of the plate.

[0053] Referring to FIG. 5, in another embodiment, the mechanism for the movement of the one or more mixing plates may comprise one or more pulleys which are turned by one or more external motors, wherein one or more belts are fixed to the one or more mixing plates, pass through the one or more pulleys, and move the one or more mixing plates longitudinally or axially within the vessel. According to other embodiments, as shown in FIGS. 6A-6B, the mechanism for the movement of the one or more mixing plates comprises two or more internal magnets attached to the one or more mixing plates and two or more movable exterior magnets on the other face of the one or more sidewalls, wherein movement of the exterior magnets moves the one or more mixing plates longitudinally or axially within the vessel. In one embodiment, the mechanisms described herein may cause the mixing plates to move vertically within the bioreactor. Alternatively, the mechanisms described herein may be oriented in some manner such that the mixing plates are configured to move laterally within the bioreactor.

[0054] In some embodiments, a single back-and-forth movement of the mixing plates is referred to herein as stroke. In some embodiments, the mechanism may be capable of moving the mixing plates at a rate of about 1-10 strokes per minute, or about 10-50 strokes per minute, or about 50-100 strokes per minute, or about 100-150 strokes per minute, or about 150-200 strokes per minute.

[0055] In some embodiments, the bioreactor may comprise more than one mixing plate. As a non-limiting example, the bioreactor may comprise about 2-5, 5-10, 10-20 or 20-50 mixing plates. In other embodiments, the spacing between the mixing plates may be uniform or varied. As a non-limiting example, the spacing between the plates may be about 1-5 cm, or about 5-10 cm, or about 10-20 cm, or about 20-50 cm.

[0056] In other embodiments, the size of the through holes may be uniform or varied. As a non-limiting example, the size of the through holes may be about 1-5 cm, or about 5-10 cm, or about 10-20 cm, or about 20-50 cm. In yet other embodiments, the mixing plates may have a uniform or varied percentage of its total area comprised of the through holes. As a non-limiting example, a mixing plate may have about 1-10%, or about 5-20%, or about 15-30%, or about 25-40%, or about 30-50%, or about 40-60%, or about 50-70%, or about 60-80%, or about 75-90% of its total area comprised of the through holes.

[0057] As used herein, the term about refers to plus or minus 10% of the referenced number.

[0058] Various modifications of the invention, in addition to those described herein, will be apparent to those skilled in the art from the foregoing description. Such modifications are also intended to fall within the scope of the appended claims. Each reference cited in the present application is incorporated herein by reference in its entirety.

[0059] Although there has been shown and described the preferred embodiment of the present invention, it will be readily apparent to those skilled in the art that modifications may be made thereto which do not exceed the scope of the appended claims. Therefore, the scope of the invention is only to be limited by the following claims. The reference numbers recited in the below claims are solely for ease of examination of this patent application, and are exemplary, and are not intended in any way to limit the scope of the claims to the particular features having the corresponding reference numbers in the drawings. In some embodiments, the figures presented in this patent application are drawn to scale, including the angles, ratios of dimensions, etc. In some embodiments, the figures are representative only and the claims are not limited by the dimensions of the figures. In some embodiments, descriptions of the inventions described herein using the phrase comprising includes embodiments that could be described as consisting of, and as such the written description requirement for claiming one or more embodiments of the present invention using the phrase consisting of is met.