Low-temperature automated storage for laboratory samples with automated access

Abstract

An automated low-temperature storage for storing laboratory samples includes a storage zone for storing the samples that is maintainable at a first temperature below 20 C.; a handling zone located above the storage zone that is maintainable at a second temperature above the first temperature and below 0 C.; a chamber laterally adjacent to the storage zone and the handling zone for storing the samples at a third temperature that is higher than the first temperature and below 0 C.; and a vertical wall separating the chamber from the handling zone and storage zone. A first opening arranged in said vertical wall connects the handling zone and the chamber; and an automated transport device is arranged at least partially in the handling zone and is configured to move the samples between the storage zone, the handling zone, and the chamber.

Claims

1. An automated low-temperature storage for storing laboratory samples, the storage comprising: a storage zone structured and arranged for storing the samples that is maintainable at a first temperature below 20 C.; a handling zone located above the storage zone that is maintainable at a second temperature above the first temperature and below 0 C.; a chamber laterally adjacent to the storage zone and to the handling zone for storing the samples at a third temperature that is higher than the first temperature and below 0 C.; a vertical wall separating said chamber from said handling zone and said storage zone, wherein a first opening is arranged in said vertical wall and connects the handling zone and the chamber; and an automated transport device being arranged at least partially in the handling zone and being structured and arranged to move the samples between the storage zone, the handling zone, and the chamber.

2. The storage of claim 1, further comprising a cooling device structured and arranged to maintain the first temperature in the storage zone, the second temperature in the handling zone, and the third temperature in the chamber.

3. The storage of claim 2, wherein at least one of: the first temperature is below 50 C. and the second temperature is below 10 C.

4. The storage of claim 1, further comprising a door which, when opened, provides user access to the chamber.

5. The storage device of claim 4, wherein the door is located in a wall arranged opposite the first opening.

6. The storage device of claim 1, further comprising at least one storage rack arranged in said chamber and structured for at least temporarily storing a plurality of the samples one above the other.

7. The storage of claim 1, further comprising a manipulator structured and arranged to automatically manipulate the samples in the chamber.

8. The storage device of claim 4, wherein said door is located on a side of said chamber opposite to said first opening.

9. The storage device of claim 7, wherein said manipulator comprises a vertical guide bar arranged in said chamber and a tray on a lift carriage displaceable along said vertical guide bar.

10. The storage device of claim 9, wherein said manipulator further comprises a transfer station and a telescopic arm, wherein said transfer station is mounted to said telescopic arm and said telescopic arm is structured to through said first opening into said handling zone.

11. The storage device of claim 9, further comprising, in said chamber, laboratory equipment for handling individual tubes of said samples, wherein said tray can be selectively brought, by vertical displacement along said guide bar, to said storage rack and said laboratory equipment.

12. The storage device of claim 9, further comprising a second opening in a peripheral housing of said chamber, wherein said loading tray is adapted and structured to transfer probes of said samples through said second opening to external hardware.

13. The storage device of claim 1, further comprising a primary housing enclosing said storage zone and said handling zone, wherein said chamber is located outside said primary housing and said first opening is arranged in said primary housing.

14. The storage device of claim 1, further comprising a primary housing enclosing said storage zone and said handling zone, wherein said chamber is located inside said primary housing.

15. The storage device of claim 1, wherein said vertical wall is arranged between said chamber and said handling zone and between said chamber and said storage zone.

16. The storage device of claim 1, wherein said vertical wall is adjacent to said chamber, adjacent to said handling zone and adjacent to said storage zone.

17. The storage of claim 1, wherein the automated transport device is movable at least within the storage zone and the handling zone.

18. An automated low-temperature storage for storing laboratory samples, the storage comprising: a vertical wall with an opening therein, a storage zone adjacent to said vertical wall and structured and arranged for storing the samples, wherein said storage zone is maintainable at a first temperature below 20 C.; a handling zone adjacent to said vertical wall and located above the storage zone, wherein said handling zone is maintainable at a second temperature above the first temperature and below 0 C.; a chamber adjacent to said vertical wall and laterally adjacent to the storage zone and to the handling zone for storing the samples at a third temperature that is higher than the first temperature and below 0 C., wherein said opening connects said handling zone and said chamber; and an automated transport device being arranged at least partially in the handling zone and being structured and arranged to move the samples between the storage zone, the handling zone, and the chamber.

19. The storage of claim 18, wherein the automated transport device is movable at least within the storage zone and the handling zone.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) The invention will be better understood and objects other than those set forth above will become apparent when consideration is given to the following detailed description thereof. Such description makes reference to the annexed drawings, wherein:

(2) FIG. 1 is a view of a complete storage;

(3) FIG. 2 is a view of the components within the primary housing of the storage;

(4) FIG. 3 shows two storage racks mounted to a mounting plate;

(5) FIG. 4 shows a storage rack with its hooks;

(6) FIG. 5 shows the lids of a carrier;

(7) FIG. 6 shows the peripheral chamber in its housing; and

(8) FIG. 7 is a top sectional view of an alternative embodiment of the peripheral chamber.

DETAILED DESCRIPTION OF THE EMBODIMENTS OF THE INVENTION

(9) The storage of FIG. 1 includes a primary housing 1 that encloses the low-temperature section of the device. It further comprises a peripheral housing 2 enclosing a peripheral chamber, two redundant cooling units 3, a liquid nitrogen container 4 as well as further external sample handling hardware 5, all of which are advantageously arranged outside primary housing 1. In addition, the device is equipped with a control unit, schematically indicated under reference numeral 12, which controls the functions of its parts, and is responsible for maintaining climate and controlling the operations of the automatic transport device as described below. Control unit 12 can e.g., be located in a separate housing or within primary housing 1.

(10) FIG. 2 shows a set-up of the components within primary housing 1. The interior space of primary housing 1 is divided into three sections, which are, in the following, called the storage zone 6, the handling zone 7 and the access zone 8.

(11) These components are described on more detail below.

(12) Storage Zone:

(13) Storage zone 6 forms the bottom most section of the interior of primary housing 1 and it is maintained at a low temperature, which is called the first temperature herein. The first temperature is a temperature below 50 C., in particular at approximately 80 C. Storage zone 6 generally receives and stores the samples for extended periods of time.

(14) Handling Zone:

(15) Handling zone 7 is located above storage zone 6 and is maintained at a second, higher temperature. The second temperature is, as mentioned, still typically below 0 C., in particular below 10 C., typically around 20 C. Handling zone 7 contains at least part of an automated transport device 9, whose purpose is to transport laboratory samples between storage zone 6 and the chamber described below. In particular, handling zone 7 advantageously holds the motors and other parts of transport device 9 that are poorly suited to operate at very low temperatures or that emit heat that might disturb the environment in storage zone 6.

(16) Access Zone:

(17) Access zone 8 forms the top section of the interior of primary housing 1 and is located above handling zone 7. Its purpose is to provide the user of the device with access to handling zone 7 and/or storage zone 6 for maintenance work.

(18) Access zone 8 is advantageously sufficiently large for a user to enter, at least in kneeling or creeping fashion, and should in that case have a height of at least 50 cm.

(19) Access zone 8 includes a door 10 closing a manhole 11. In the embodiment of FIG. 2, access zone 8 is elongate and door 10 is located at one vertical end wall of access zone 8.

(20) The floor of access zone 8 is formed by a separating wall 14, which separates access zone 8 from handling zone 7. It comprises several gas-proof panels 15 located in a frame 16 that are structured to carry the weight of the user.

(21) Each panel 15 can be lifted from frame 16 in order to form an access opening from access zone 8 into handling zone 7.

(22) It must be noted that the panels 15 are only one of various possible embodiments of separating wall 14. For example, separating wall 14 may also comprise trapdoors or any other suitable structure that allows at least partial removal in order to provide access from access zone 8 to handling zone 7 and/or to storage zone 6.

(23) The components within primary housing 1 further include an air dryer 20 arranged and structured to dry air in access zone 8. It can be used to remove humidity from access zone 8, such as humidity introduced when the user opens door 10 or exhales into the access zone 8.

(24) Advantageously, at least one ventilator 18, or another device for enabling and disabling an atmosphere exchange through separating wall 14, such as a closeable flap or door, is arranged in separating wall 14 in order to optionally circulate air between access zone 8 and handling zone 7 and storage zone 6, thereby allowing to use dryer 20 for drying handling zone 7 and storage zone 6, e.g., before cooling storage zone 6 down to its operating temperature.

(25) In normal operation of the device, access zone 8 is, as mentioned, maintained at an access temperature, which is well above the temperature of storage zone 6. It may also be higher than the temperature in handling zone 7.

(26) Sample Storage:

(27) As shown in FIG. 2, several carriers 22 extend from above into storage zone 6. Each carrier 22 is suspended on a carriage located in handling zone 7. Each such carriage is displaceable therein along a longitudinal horizontal direction of the device.

(28) Each carrier 22 comprises two spaced apart, parallel, vertical side walls 24a, 24b, see FIGS. 2 and 5. Further, each carrier 22 comprises a vertical mounting plate 25, extending between the side walls 24a, 24b and being arranged perpendicularly thereto.

(29) Mounting plate 25, which is illustrated in even greater detail in FIG. 3, is a flat sheet metal plate having a plurality of openings 26 formed therein.

(30) To receive the samples, a plurality of storage racks 27 can be hooked to both sides of each mounting plate 25. As can best be seen in FIGS. 3 and 4, each storage rack 27 comprises a plurality of storage locations above each other, with each storage location being formed by two lateral ledges 29a mounted to lateral vertical side walls 29b of the storage rack 27.

(31) For hooking the storage racks 27 onto the mounting plates 25, each storage rack 27 is provided with several hooks 30 at its side facing mounting plate 25, see FIG. 3, 4. In the present embodiment, the hooks 30 are formed as projections of the side walls 27b of the racks, with each hook of the left side wall being located at a height in the middle of the two neighboring hooks of the right side wall and vice versa, such that the hooks of two storage racks 27 arranged directly opposite each other on mounting plate 25, as shown in FIG. 3, do not interfere with each other.

(32) To mount a storage rack 30 to mounting plate 25, its hooks 30 are inserted into a suitable set of the openings 26 and engage the same.

(33) As can be seen in FIG. 3, the openings 26 are tapered towards their bottom ends, such that they laterally guide and finally confine the hooks 30 engaged therein.

(34) In addition, and as can be seen from FIG. 4, each hook comprises at least one guiding surface 32, in the present case formed by the inclined bottom edge thereof, which faces its respective storage rack 27 and is inclined to the vertical direction of the storage rack 27 (with the term inclined expressing the fact that the angle between the vertical direction and guiding surface 32 is clearly neither 0 nor 90 but somewhere in between). When a hook 30 is inserted into one of the openings 26 and then lowered, if guiding surface 32 abuts against the bottom edge of the opening, it pulls the hook into the opening and thereby the storage rack against the mounting plate.

(35) Similarly, the top edge 33 of each hook 30, which faces away from its storage rack 27, is also inclined in respect to the vertical direction of storage rack 27 and serves to guide hook 30 into its respective opening 26 in case it abuts against the top edge of the opening.

(36) As can further be seen from FIG. 5, each carrier 22 comprises a pair of lids 34a, 34b above the racks 27 carried by the carrier (only one of the racks 27 is shown in FIG. 5). The lids 34a, 34b serve to separate storage zone 6 at least partially from handling zone 7.

(37) Each lid is a metal plate extending between the side walls 24a, 24b of its carrier and mounted thereto by hinges 35 defining a horizontal hinge axis 36 extending perpendicular to the side walls 24a, 24b. In a closed position, the lids 34a, 34b are arranged horizontally. To open a lid 34a, 34b, the user seizes a bent-up edge region 37 of the lid 34a, 34b and pivots it about hinge axis 36.

(38) The purpose of the lids 34a, 34b is to insulate storage zone 6 at least partially from the hotter handling zone 7, and in particular from the heat of the motors of the transport device located in handling zone 7.

(39) Transport Device:

(40) The operation and design of transport device 9 substantially correspond to those of the transport device described in EP 1 972 874 and is not repeated in detail here.

(41) In short, transport device 9 comprises a tray 40 mounted to a vertical guide 41, see FIG. 2. Guide 41 is suspended from a carriage running in rails in handling zone 7 and extends, while the carriage of the transport device 9 is being run along its rails, into a lateral gap formed between one set of side walls 24b of the carriers 22 and a neighboring vertical wall of primary housing 1. To access a storage position in the storage racks 27, the carriers 22 are horizontally displaced in such a manner that a gap forms between them at the location of storage position. Then, guide 41 is horizontally displaced into this gap while tray 40 is vertically displaced to the appropriate height in order to access the storage position.

(42) Details of this mechanism are, as mentioned, described in EP 1 972 874.

(43) Peripheral Chamber:

(44) Advantageously, the present device is provided with a peripheral chamber laterally adjacent to storage zone 6 and handling zone 7 and separated from them by a vertical wall. The peripheral chamber serves for temporarily storing the probes, e.g. when probes are to be introduced into the storage, removed from the storage, or handled by processing equipment or analytical equipment. As mentioned, the handling zone 8 is at a third temperature higher than the first (lowest) temperature, e.g. at 20 C. The third temperature will generally be similar or equal to the second temperature, i.e., to the temperature in handling zone 7.

(45) In the embodiment shown in FIG. 1, and depicted in detail in FIG. 6, peripheral chamber 50 is located in peripheral housing 2, i.e., outside primary housing 1. Peripheral housing 2 is mounted with its back side against primary housing 1, and an opening 51 is provided that connects peripheral chamber 50 to handling zone 7. Transport device 9, which can include a first part located in handling zone 7 and a second part located in peripheral chamber 50, is adapted to move the samples from storage zone 6 through handling zone 7 into peripheral chamber 50 and back.

(46) Peripheral housing 2 includes a user-operatable door 52, which, when opened, provides user access to peripheral chamber 50. Door 52 is located at the front side of peripheral housing 2, i.e., in the wall of peripheral housing 2 that is opposite to opening 51.

(47) Advantageously, at least one storage rack 54 for temporarily storing a plurality of samples above each other is located in peripheral chamber 50. In the embodiment of FIG. 6, a plurality of such storage racks 54 are located on a rotatable carousel table 69.

(48) The transport device 9 includes a manipulator 55 for automatically manipulating the samples in peripheral chamber 50. Manipulator 55 comprises a loading tray 56 on a lift carriage 56a, which can be displaced along a vertical guide bar 57. Tray 56 can further be rotated about a vertical axis. Manipulator 55 further comprises a transfer station 58. Transfer station 58 is mounted to a telescopic arm 59, which can be operated to extend through opening 51 into handling zone 7, where a sample can be loaded/unloaded to/from transfer station 58. On the other hand, arm 59 can be retracted, in which position loading tray 56 can pick up and deposit probes from/onto transfer station 58.

(49) Once it is on loading tray 56, the sample can be displaced vertically, either to bring it to the location of various laboratory equipment, such as the stations 60 for handling individual tubes of the probes as shown in FIG. 6, or it can be brought to the location of the storage racks 54, where the sample can be stored.

(50) As can be seen in FIG. 6, peripheral housing 2 may comprise a further opening 62, e.g., in a lateral side wall thereof, which allows to transfer probes to external sample hardware 5 (see FIG. 1), again by loading tray 56.

(51) An alternative embodiment is shown in FIG. 7, where peripheral chamber 50 is formed within primary housing 1. In this embodiment, two storage racks 54 are located within storage location 50, each of which can receive several samples and can be accessed by transport device 9.

(52) Transport device 9 comprises a vertical guide bar 57 and a tray 56 vertically displaceable along guide bar 57 as well as horizontally pivotal about a vertical axis in order to access the two storage racks 54. Guide bar 57 and tray 56 may be the same parts as guide 41 and tray 40 of FIG. 2, or they may be formed by separate components.

(53) A vertical wall 65 separates peripheral chamber 50 from handling zone 7 and storage zone 6. An opening (not shown) is arranged in wall 65 for providing tray 56 with access to the storage racks 54. A door 52 is located opposite to this opening and is e.g. used for replacing the storage racks 54.

(54) Climate Control:

(55) The low temperature within the storage is primarily maintained by the cooling units 3 under control of control unit 12. In addition, liquid nitrogen container 4 is connected through a first duct 70 and a second duct 71 to the interior primary housing 1, advantageously directly to storage zone 6.

(56) First duct 70 is connected to container 4 in such a way that it collects the evaporated nitrogen, which is produced by the continuously simmering nitrogen in container 4, and transports it to storage zone 6. Advantageously, duct 70 remains always open.

(57) Second duct 71 is connected to container 4 in such a way that it conveys liquid nitrogen into handling zone 6 or any sections of the device thermally connected to handling zone 6. Second duct 71 can be closed by a valve 72. Valve 72 can be controlled manually and/or by control unit 12.

(58) In normal operation, valve 72 is closed. The natural evaporation rate of the nitrogen in nitrogen container 4 provides a sufficient amount of gaseous nitrogen continuously flowing through duct 70 into storage zone 6 in order to maintain an overpressure therein. Since the evaporated nitrogen is dry, this prevents humidity from entering storage zone 6 is forming ice deposits therein.

(59) Instead of a nitrogen container 4, another gas source providing sufficiently dry gas can be used to maintain a dry atmosphere under slight overpressure within storage zone 6.

(60) A further important function of nitrogen container 4 is to provide reliable cooling for storage zone 6 even if the cooling units 3 fail. Since the liquid nitrogen is very cold, while it lasts it prevents the temperature in storage zone 6 to rise even if the cooling units 3 are unable to cool it. Hence, in case of a failure of the cooling units 3, e.g., due to a power loss, valve 72 can be opened manually or automatically and liquid nitrogen can be guided through duct 71 for cooling storage zone 6.

(61) In other words, control unit 12 is adapted and structured to control the flow of the evaporated nitrogen from nitrogen container 4 to storage zone 6 such that In normal operation, the overpressure in storage zone 6 is maintained by the evaporated nitrogen from liquid nitrogen container 4, while the first in storage zone 6 is maintained by the cooling unit(s) 3, and upon a failure of the cooling unit(s) 3, the overpressure and the temperature in storage zone 6 are maintained by the evaporated nitrogen.

(62) This control procedure is programmed into control unit 12 as a method for controlling the storage. Such a method is also part of the present invention.

(63) The term sample as used herein encompasses the substance to be stored and, optionally, a container for one or more such substances. For example, the sample can be a bottle containing a single substance, or it can be a microtiter plate or a tube carrier carrying a plurality of substances.

(64) While there are shown and described presently preferred embodiments of the invention, it is to be distinctly understood that the invention is not limited thereto but may be otherwise variously embodied and practiced within the scope of the following claims.