METHOD FOR SEPARATING GEOMETRICAL ISOMER

Abstract

The present invention provides a method for separating a compound represented by Formula (1) or (2) [in the formula, P.sup.1 is a hydrogen atom or a protective group of a hydroxyl group, R.sup.1 is a linear or branched C.sub.1-6 alkyl group that may be substituted with a phenyl group, A is an alkenylene group, and R.sup.2 is a hydroxyl group, a C.sub.1-3 alkoxy group, a mono(C.sub.1-3 alkyl)amino group, or a di(C.sub.1-3 alkyl)amino group] from a geometrical isomer thereof, in which the geometrical isomer is a geometrical isomer in a double bond included in A, the method including processing a mixture containing the compound and the geometrical isomer thereof by a chromatographic method using an acidic functional group-modified silica gel as a stationary phase.

##STR00001##

Claims

1. A method for separating a compound represented by Formula (1) or (2) from a geometrical isomer thereof, in which the geometrical isomer is a geometrical isomer in a double bond included in A, the method comprising: processing a mixture containing the compound and the geometrical isomer thereof by a chromatographic method using an acidic functional group-modified silica gel as a stationary phase, ##STR00016## in the formulae, P.sup.1 and P.sup.2 are each independently a hydrogen atom or a protective group of a hydroxyl group, R.sup.1 is a linear or branched C.sub.1-6 alkyl group that may be substituted with a phenyl group, A is a C.sub.3-10 alkenylene group, R.sup.2 is a hydroxyl group, a C.sub.1-3 alkoxy group, a mono(C.sub.1-3 alkyl)amino group, or a di(C.sub.1-3 alkyl)amino group, and ##STR00017## is a single bond or a double bond.

2. The method according to claim 1, wherein A is ##STR00018##

3. The method according to claim 1, wherein R.sup.1 is ##STR00019##

4. The method according to claim 1, wherein P.sup.1 is a hydrogen atom or a 2-tetrahydropyranyl group.

5. The method according to claim 1, wherein the acidic functional group-modified silica gel is a silica gel modified with a carboxy group or a sulfo group.

6. The method according to claim 2, wherein R.sup.1 is ##STR00020##

7. The method according to claim 2, wherein P.sup.1 is a hydrogen atom or a 2-tetrahydropyranyl group.

8. The method according to claim 3, wherein P.sup.1 is a hydrogen atom or a 2-tetrahydropyranyl group.

9. The method according to claim 2, wherein the acidic functional group-modified silica gel is a silica gel modified with a carboxy group or a sulfo group.

10. The method according to claim 3, wherein the acidic functional group-modified silica gel is a silica gel modified with a carboxy group or a sulfo group.

11. The method according to claim 4, wherein the acidic functional group-modified silica gel is a silica gel modified with a carboxy group or a sulfo group.

Description

DESCRIPTION OF EMBODIMENTS

[0018] A detailed description will be given below of the present invention.

[0019] One embodiment of the present invention is method for separating a compound represented by Formula (1) or (2) from a geometrical isomer thereof, in which the geometrical isomer is a geometrical isomer in a double bond included in A, the method including processing a mixture containing the compound and the geometrical isomer thereof by a chromatographic method using an acidic functional group-modified silica gel as a stationary phase.

##STR00008##

[0020] [in the formula, P.sup.1 and P.sup.2 are each independently a hydrogen atom or a protective group of a hydroxyl group, R.sup.1 is a linear or branched C.sub.1-6 alkyl group that may be substituted with a phenyl group, A is a C.sub.3-10 alkenylene group, R.sup.2 is a hydroxyl group, a C.sub.1-3 alkoxy group, a mono(C.sub.1-3 alkyl)amino group, or a di(C.sub.1-3 alkyl)amino group, and

##STR00009##

is a single bond or double bond.]

[0021] The combination of two or more geometrical isomers as separation targets in the method according to the present embodiment is a compound represented by Formula (1) or (2), which is in a cis-isomer and trans-isomer relationship in the double bond included in A. In a case where the double bond is also present in the other side chain (the side chain having R.sup.1), it is possible for a total of four types of geometrical isomers to be present.

##STR00010##

[0022] P.sup.1 and P.sup.2 are each independently a hydrogen atom or a protective group of a hydroxyl group. The protective group of the hydroxyl group is a substituent used for the purpose of protecting the hydroxyl group to prevent reaction with a reactant in an organic synthetic reaction. The protective group of the hydroxyl group is not particularly limited and examples thereof include acetal-based protective groups such as a methoxymethyl group, an ethoxyethyl group, a benzyloxymethyl group, or a tetrahydropyranyl group, ether-based protective groups such as a benzyl group, a p-methoxybenzyl group, or a p-nitrobenzyl group, acyl-based protective groups such as an acetyl group, a pivaloyl group, a benzoyl group, and a p-methoxybenzoyl group, and silyl-based protective groups such as a trimethylsilyl group, a triethylsilyl group, a tert-butyldimethyl group, a triphenylsilyl group, and a phenyldimethylsilyl group.

[0023] R.sup.1 is a linear or branched C.sub.1-6 alkyl group that may be substituted with a phenyl group. A linear or branched C.sub.1-6 alkyl group is an alkyl group having 1 to 6 carbon atoms and specific examples thereof include a methyl group, an ethyl group, a 1-propyl group, a 2-propyl group, a 1-butyl group, a 2-butyl group, a tert-butyl group, a 1-pentyl group, a 2-pentyl group, a 3-pentyl group, a 1,1-dimethyl propyl group, 1-hexyl group, 2-hexyl group, a 3-hexyl group, and the like. R.sup.1 may be the linear or branched C.sub.1-6 alkyl group described above substituted with a phenyl group.

[0024] A is a C.sub.3-10 alkenylene group and specific examples thereof include a propenylene group, a butenylene group, a pentenylene group, a hexenylene group, a heptenylene group, an octenylene group, a nonylene group, or a decenylene group. The position of the double bond in A is not particularly limited. According to the separation method according to the present embodiment, it is possible to separate the geometrical isomers (cis-isomer and trans-isomer) in the double bond.

[0025] R.sup.2 is a hydroxyl group, a C.sub.1-3 alkoxy group, a mono(C.sub.1-3 alkyl)amino group or a di(C.sub.1-3 alkyl)amino group. A C.sub.1-3 alkoxy group is a group in which an alkyl group having 1 to 3 carbon atoms is substituted for an oxygen atom and specific examples thereof include a methoxy group, an ethoxy group, a 1-propoxy group, or a 2-propoxy group. A mono(C.sub.1-3 alkyl)amino group is a group in which one alkyl group having 1 to 3 carbon atoms is substituted for a nitrogen atom and specific examples thereof include a monomethylamino group, a monoethylamino group, a mono(1-propyl)amino group, a mono(2-propyl)amino group, and the like. A di(C.sub.1-3 alkyl)amino group is a group in which two alkyl groups having 1 to 3 carbon atoms are substituted for a nitrogen atom and specific examples thereof include a dimethyl amino group, a diethyl amino group, a di(1-propyl)amino group, a di(2-propyl)amino group, an ethyl(methyl)amino group, and the like.

[0026] The separation method according to the present embodiment includes treating the mixture of geometrical isomers by a chromatographic method using an acidic functional group-modified silica gel as a filling material (stationary phase).

[0027] The acidic functional group-modified silica gel used as the stationary phase in the chromatographic method may be any silica gel modified with acidic functional groups. Examples of acidic functional group-modified silica gels include carboxy group-modified silica gels, sulfo group-modified silica gels, and the like. The chromatographic method described above is preferably normal-phase chromatography.

[0028] The shape of the acidic functional group-modified silica gel may be spherical or crushed, preferably spherical. Spherical silica gel has a constant surface area and is able to be packed uniformly in a column, thus, the degree of separation is further improved when carrying out separation by the chromatographic method.

[0029] The average particle size of the acidic functional group-modified silica gel may be 3 m to 500 m, preferably 5 m to 300 m, and more preferably 30 m to 200 m.

[0030] The length of the column may he 5 cm to 200 cm, preferably 10 cm to 150 cm, and more preferably 15 cm to 100 cm. A column length of 15 cm or longer improves the degree of separation and widens the eluent selection range.

[0031] For the eluent (mobile phase), it is possible to use organic solvents well known to a person skilled in the art. Examples of organic solvents include aliphatic hydrocarbon-based solvents such as pentane, hexane, and heptane; aromatic hydrocarbon-based solvents such as toluene; halogenated hydrocarbon-based solvents such as dichloromethane and chloroform; ester-based solvents such as ethyl acetate and propyl acetate; alcohol-based solvents such as methanol, ethanol, and 2-propanol, and the like. It is possible to select these solvents as appropriate in consideration of the solubility of the crude product for purification which is the separation target and also to mix and use these solvents in any ratio in consideration of mutual compatibility. Examples of mixed solvents include binary mixed solvents such as a combination of hexane and ethanol, hexane and isopropanol, or hexane and ethyl acetate, and ternary mixed solvents such as a combination of hexane, methanol, and isopropanol.

[0032] In the present specification, separating a compound represented by Formula (1) or (2) from the geometrical isomer thereof means that, in a case where the content of the desired compound is 100, the content of the corresponding geometrical isomer is 2 or less, preferably 1 or less, and more preferably 0.5 or less. In addition, the purity of the compound represented by Formula (1) or (2) after separation may be 90% or more, 95% or more is preferable, and 97% or more, 98% or more, or 99% or more is more preferable.

EXAMPLES

[0033] A more detailed description will be given below of the present invention using Examples and Comparative Examples.

[0034] Abbreviations used in the Examples and the like are to be understood with the meanings well-known to a person skilled in the art, unless otherwise noted. For example, the meanings of some abbreviations are given below.

[0035] THP: 2-tetrahydropyanyl

[0036] Ph: phenyl

Example 1

[0037] Purification of (Z)-7-[(1R,2R,3R,5 S)-3,5-dihydroxy-2-[(3R)-3-hydroxy-5-phenylpentyl]cyclopentyl]hepto-5-enoic acid ((Z)-IFL-FA)

##STR00011##

Example 1a

[0038] A crude product for purification (EZ mixture of IFL-FA, 238 mg) was dissolved in dichloromethane and purified by a chromatographic method according to the following separation conditions and the fractions where the E-isomer was not detected under the following analysis conditions were collected to obtain the Z-isomer (yield amount: 206 mg, yield rate: 90%).

<Separation Conditions>

[0039] Eluent: Hexane:Isopropanol=10:1

[0040] Filling material: CROMATOREX COOH MB100-40/75 (trade name, manufactured by Fuji Silysia Chemical Ltd., spherical silica gel (average particle size 40 m to 75 m, pore diameter 10 nm))

[0041] Preparation volume: 1 to 2 mL/fraction

<Analysis Conditions>

[0042] Column: YMC-Pack SIL (trade name, manufactured by YMC Co., Ltd., inner diameter: 4.6 mm, length: 25 cm)

[0043] Mobile phase: Hexane:Ethanol:Acetic acid=91:9:0.05

[0044] Flow rate: 1 mL per minute

[0045] Detection wavelength: 215 nm

[0046] Injection volume: 20 L

[0047] The content ratio of each isomer before and after separation is shown in Table 1. The content ratio of each isomer was calculated based on the area under the curve of the chromatogram obtained under the analysis conditions described above.

[0048] (E)-IFL-FA is (E)-7-[(1R,2R,3R,5S)-3,5-dihydroxy-2-[(3R)-3-hydroxy-5-phenylpentyl]cyclopentyl]hepto-5-enoic acid.

TABLE-US-00001 TABLE 1 Before separation After separation (Z)-IFL-FA 74.0% 82.0% (E)-IFL-FA 1.1% 0.0%

Example 1b

[0049] A crude product for purification (EZ mixture of IFL-FA, 224 mg) was dissolved in dichloromethane and purified by a chromatographic method according to the following separation conditions and the fractions where the E-isomer was not detected under the following analysis conditions were collected to obtain the Z-isomer (yield amount: 142 mg, yield rate: 68%).

<Separation Conditions>

[0050] Eluent: Hexane:Isopropanol=10:1

[0051] Filling material: CROMATOREX SO3H MB100-40/75 (trade name, manufactured by Fuji Silysia Chemical Ltd., spherical silica gel (average particle size 40 m to 75 m, pore diameter 10 nm))

[0052] Preparation volume: 1 to 2 mL/fraction

<Analysis Conditions>

[0053] Column: YMC-Pack SIL (trade name, manufactured by YMC Co., Ltd., inner diameter: 4.6 mm, length: 25 cm)

[0054] Mobile phase:Hexane:Ethanol:Acetic acid=91:9:0.05

[0055] Flow rate: 1 mL per minute

[0056] Detection wavelength: 215 nm

[0057] Injection volume: 20 L

[0058] The content ratio of each isomer before and after separation is shown in Table 2. The content ratio of each isomer was calculated based on the area under the curve of the chromatogram obtained under the analysis conditions described above. The content ratio of the E-isomer was 0.0% and the Z-isomer was obtained with high purity.

TABLE-US-00002 TABLE 2 Before separation After separation (Z)-IFL-FA 74.0% 99.2% (E)-IFL-FA 1.1% 0.0%

Example 2

[0059] Purification of (Z)-7-[(1R,2R,3R,5S)-5-hydroxy-2-[(3R)-5-phenyl-3-[(tetrahydro-2H-pyran-2-yl)oxy]pentyl]-3-[(tetrahydro-2H-pyran-2-yl)oxy]cyclopentyl]hepto-5-enoic acid ((Z)-IFL-PPF)

##STR00012##

[0060] A crude product for purification (EZ mixture of IFL-PPF, 392 mg) was dissolved in a mixture of hexane and ethyl acetate and purified by a chromatographic method according to the following separation conditions and the fractions having 0.5% or less of the E-isomer were collected under the following analysis conditions to obtain the Z-isomer (yield amount: 99 mg, yield rate: 68%). Analysis was performed after concentrating approximately 0.1 mL of the fraction, dissolving the residue in 1 mL of 2-propanol, adding a catalytic amount of paratoluenesulfonic acid monohydrate and carrying out a reaction at room temperature for approximately 2 hours to deprotect and derivatize to IFL-FA (refer to Example 1).

<Separation Conditions>

[0061] Eluent: Hexane:ethyl acetate=3:1-3:7

[0062] Filling material: CROMATOREX SO3H MB100-40/75 (trade name, manufactured by Fuji Silysia Chemical Ltd., spherical silica gel (average particle size: 40 m to 75 m, pore diameter 10 nm))

[0063] Preparation volume: 1 to 2 mL/fraction

<Analysis Conditions>

[0064] Column: YMC-Pack SIL (trade name, manufactured by YMC Co., Ltd., inner diameter: 4.6 mm, length: 25 cm)

[0065] Mobile phase: Hexane:Ethanol:Acetic acid=91:9:0.05

[0066] Flow rate: 1 mL per minute

[0067] Detection wavelength: 215 nm

[0068] Injection volume: 20 L

[0069] The content ratio of each isomer before and after separation is shown in Table 3. The content ratio of each isomer was calculated based on the area under the curve of the chromatogram. (E)-IFL-PPF is (E)-7-[(1R,2R,3R,5S)-5-hydroxy-2-[(3R)-5-phenyl-3-[(tetrahydro-2H-pyran-2-yl)oxy]pentyl]-3-[(tetrahydro-2H-pyran-2-yl)oxy]cyclopentyl]hepto5-enoic acid. The E-isomer included in the Z-isomer after separation was 0.2% when measured under the above analysis conditions. In addition, it was also possible to separate triphenylphosphine, which was included to a large extent in the crude product for purification.

TABLE-US-00003 TABLE 3 Before separation After separation (Z)-IFL-PPF 17.3% 99.2% (E)-IFL-PPF 2.0% 0.2% Triphenylphosphine 78.0% 0.5% oxide

Example 3

[0070] Purification of propan-2-yl (Z)-7-[(1R,2R,3R,5S)-3,5-dihydroxy-2-[(3R)-3-hydroxy-5-phenylpentyl]cyclopentyl]hepto-5-enoate ((Z)-latanoprost)

##STR00013##

Example 3a

[0071] The crude product for purification (EZ mixture of latanoprost, 200 mg) was dissolved in a mixture of hexane and ethyl acetate and purified by the chromatographic method according to the following separation conditions and the fractions having 0.5% or less of the E-isomer were collected under the following analysis conditions to obtain the Z-isomer (yield amount: 94 mg, yield rate: 47%).

<Separation Conditions>

[0072] Eluent: Hexane:Ethyl acetate=3:1-3:7

[0073] Filling material: CROMATOREX COOH MB100-40/7 (trade name, manufactured by Fuji Silysia Chemical Ltd., spherical silica gel (average particle size: 40 m to 75 m, pore diameter: 10 nm))

[0074] Preparation volume: 1 to 2 mL/fraction

<Analysis Conditions>

[0075] Column: Spherisorb Silica (trade name, manufactured by Waters Corporation, inner diameter: 4.6 mm, length: 25 cm)

[0076] Mobile phase: Hexane:Ethanol:Acetic acid=91:9:0.05

[0077] Flow rate: 1 mL per minute

[0078] Detection wavelength: 215 nm

[0079] Injection volume: 20 m

[0080] The content ratio of each isomer before and after separation is shown in Table 4. The content ratio of each isomer was calculated based on the area under the curve of the chromatogram. (E)-Latanoprost is a propan-2-yl(E)-7-[(1R,2R,3R,5S)-3,5-dihydroxy-2-[(3R)-3-hydroxy-5-phenylpentyl ]cyclopentyl]hepto-5-enoate. The content ratio of the E-isomer was 0.4% under the above analysis conditions and it was possible to obtain the Z-isomer with high purity.

TABLE-US-00004 TABLE 4 Before separation After separation (Z)-Latanoprost 96.3% 98.7% (E)-Latanoprost 1.5% 0.4%

Example 3b

[0081] The crude product for purification (EZ mixture of IFL, 200 mg) was dissolved in a mixture of hexane and ethyl acetate and purified by the chromatographic method according to the following separation conditions and the fractions having 0.5% or less of the E-isomer were collected under the following analysis conditions to obtain the Z-isomer (yield amount: 64 mg, yield rate: 32%).

<Separation Conditions>

[0082] Eluent: Hexane:Ethyl acetate=3:1 to 2:3

[0083] Filling material: CROMATOREX SO3H MB100-40/75 (trade name, manufactured by Fuji Silysia Chemical Ltd., spherical silica gel (average particle size: 40 m to 75 m, pore diameter: 10 nm))

[0084] Preparation volume: 1 to 2 mL/fraction

<Analysis Conditions>

[0085] Column: Spherisorb Silica (trade name, manufactured by Waters Corporation, inner diameter: 4.6 mm, length: 25 cm)

[0086] Mobile phase: Hexane:Ethanol:Acetic acid=91:9:0.05

[0087] Flow rate: 1 mL per minute

[0088] Detection wavelength: 215 nm

[0089] Injection volume: 20 L

[0090] The content ratio of each isomer before and after separation is shown in Table 5. The content ratio of each isomer was calculated based on the area under the curve of the chromatogram. The content ratio of the E-isomer was 0.5% and it was possible to obtain the Z-isomer with high purity.

TABLE-US-00005 TABLE 5 Before separation After separation (Z)-Latanoprost 98.2% 99.5% (E)-Latanoprost 1.6% 0.5%

Example 4

[0091] Purification of (E)-7-((1R,2R,3R)-3-hydroxy-2((3S,5S,E)-3-hydroxy-5-methylnon-1-en-1-yl)-5-oxocyclopentyl-2-enoic acid ((E)-IEL)

##STR00014##

[0092] The crude product for purification (EZ mixture of IEL, 33 mg) was dissolved in dichloromethane and purified by the chromatographic method according to the following separation conditions and the fractions where the E-isomer was not detected under the following analysis conditions were collected to obtain the E-isomer (yield amount: 13 mg, yield rate: 56%).

<Separation Conditions>

[0093] Fluent: Hexane:Ethanol=10:1

[0094] Filling material: CROMATOREX COOH SMB100-10 (trade name, manufactured by Fuji Silysia Chemical Ltd., Spherical silica gel (average particle size: 10 m, pore diameter: 10 nm))

[0095] Preparation volume: 1 to 2 mL/fraction

<Analysis Conditions>

[0096] Column: Develosil ODS-5 (trade name, manufactured by Nomura Chemical Co., Ltd., inner diameter: 4.6 mm, length: 15 cm)

[0097] Mobile phase: 0.02 M potassium dihydrogen phosphate buffer: acetonitrile:2-propanol=9:5:2

[0098] Flow rate: 1 mL per minute

[0099] Detection wavelength: 215 nm

[0100] Injection volume: 20 L

[0101] The content ratios of each isomer before and after separation are shown in Table 6. The content ratio of each compound was calculated based on the area under the curve of the chromatogram. (Z)-IEL is (Z)-7-((1R,2R,3R)-3-hydroxy-2-((3S,5S,E)-3-hydroxy-5-methylnon-1-e n-1-yl)-5-oxocyclopentyl)hepto-2-enoic acid, and AT-IEL is (E)-7((1R,2S)-2((3S,5S,E)-3-hydroxy-5-methylnon-1-en-1-yl)-5-oxoc yclopent-3-en-1-yl)hepto-2-enoic acid. The content ratio of the E-isomer included in the Z-isomer after separation was 0.0% under the above analysis conditions. In addition, it was also possible to separate AT-IEL, which was included to a large extent in the crude product for purification.

TABLE-US-00006 TABLE 6 Before separation After separation (E)-IEL 74.8% 93.6% (Z)-IEL 1.6% 0.0% AT-IEL 10.0% 0.4%

Comparative Example 1

[0102] Purification of (Z)-7-[(1R,2,3,5S)-5-hydroxy-2-[(3R)-5-phenyl-3-[(tetrahydro-2H-pyran-2-yl)oxy]pentyl]-3-[tetrahydro-2H-pyran-2-yl)oxy]cyclopentyl]hepto-5-enoic acid ((Z)-IFL-PPF)

##STR00015##

[0103] The crude product for purification (EZ mixture, 75.8 g) was dissolved in a mixed solvent of hexane and ethyl acetate and purified by the chromatographic method according to the following separation conditions to obtain the (Z) isomer (yield amount: 45.3 g, yield rate: 96%). In all recovered fractions, (E)-latanoprost was detected and was not able to be separated. Analysis was performed by concentrating approximately 0.1 mL of the fractions, dissolving the residue in 1 mL of 2-propanol, adding a catalytic amount of paratoluenesulfonic acid monohydrate, and carrying out a reaction at room temperature for approximately 2 hours to deprotect and derivatize to IFL-FA (refer to Example 1).

<Separation Conditions>

[0104] Eluent: Hexane:ethyl acetate=3:2

[0105] Filling material: BW-300S (trade name, manufactured by Fuji Silysia Chemical Ltd., crushed silica gel (average particle size: 38 m to 75 m, pore diameter: 6 nm))

[0106] Preparation volume: 20-50 mL/fraction

<Analysis Conditions>

[0107] Column: YMC-Pack SIL (trade name, manufactured by YMC Co., Ltd., inner diameter: 4.6 mm, length: 25 cm)

[0108] Mobile phase: Hexane:Ethanol:Acetic acid=91:9:0.05

[0109] Flow rate: 1 mL per minute

[0110] Detection wavelength: 215 nm

[0111] Injection volume: 20 L

[0112] The content ratio of each isomer before and after separation is shown in Table 7. The content ratio of each isomer was calculated based on the area under the curve of the chromatogram.

TABLE-US-00007 TABLE 7 Before separation After separation (Z)-IFL-PPF 63% 97.2% (E)-IFL-PPF 1.9% 1.9%

Comparative Example 2

[0113] Purification of propan-2-yl (Z)-7-[(1R,2R,3R,5S)-3,5-dihydroxy-2-[(3R)-3-hydroxy-5-phenylpentyl]cyclopentyl]hepto-5-enoate ((Z)-latanoprost)

[0114] The crude product for purification (EZ mixture of latanoprost, 17.3 g) was dissolved in a mixture of hexane and ethyl acetate and purified by the chromatographic method according to the following separation conditions to obtain (Z)-latanoprost (yield amount: 14.6 g, yield rate: 88%). In all fractions including (Z)-latanoprost, (E)-latanoprost was detected under the following analysis conditions and was not able to be separated.

<Separation Conditions>

[0115] Fluent: Hexane:Ethyl acetate=3:2

[0116] Filling material: Silica gel 60 high-purity product (trade name, manufactured by Kanto Chemical Co., Inc.)

[0117] Preparation volume: 20-50 ml/fraction

<Analysis Conditions>

[0118] Column: Spherisorb Silica (trade name, manufactured by Waters Corporation, inner diameter: 4.6 mm, length: 25 cm)

[0119] Mobile phase: Hexane:Ethanol:Acetic acid=91:9:0.05

[0120] Flow rate: 1 mL per minute

[0121] Detection wavelength: 215 nm

[0122] Injection volume: 20 L

[0123] The content ratio of each isomer before and after separation is shown in Table 8. The content ratio of each isomer content was calculated based on the area under the curve of the chromatogram.

TABLE-US-00008 TABLE 8 Before separation After separation (Z)-Latanoprost 95.5% 98.1% (E)-Latanoprost 1.9% 1.9%

Reference Example 1

[0124] Purification of (Z)-7(1R,2R,3R)-3-hydroxy-2((S,E)-3-hydroxyocto-1-en-1-yl)-5-oxocyclopentyl)hepto-5-enoic acid (PGE.sub.2, dinoprostone).

[0125] The crude product for purification (mixture of PGE.sub.2 and PGA.sub.2, 35 mg) was dissolved in dichloromethane and purified by the chromatographic method according to the following separation conditions and the fractions not including PGA2 were collected under the following analysis conditions to obtain PGE2 (yield amount: 20 mg, yield rate: 100%).

<Separation Conditions>

[0126] Eluent Hexane:Ethanol=10:1

[0127] Filling material: CROMATOREX COOH SMB100-10 (trade name, manufactured by Fuji Silysia Chemical Ltd., Spherical silica gel (average particle size: 10 m, pore diameter: 10 nm))

[0128] Preparation volume: 1 to 2 mL/fraction.

[0129] The content ratios of each isomer before separation and after separation are shown in Table 9. The content ratio of each isomer was calculated based on the area under the curve of the chromatogram obtained under the following analysis conditions.

<Analysis Conditions>

[0130] Column: L-Column 2 ODS (trade name, manufactured by Chemicals Evaluation and Research Institute, Japan, inner diameter: 4.6 mm, length: 25 cm)

[0131] Mobile phase:Methanol:0.2 volume % acetic acid solution=58:42

[0132] Flow rate: 1 mL per minute

[0133] Detection wavelength: 210 nm

[0134] Injection volume: 20 L

TABLE-US-00009 TABLE 9 Before separation After separation PGE.sub.2 71.2% 93.6% PGA.sub.2 13.5% 1.1%